TY  - CONF
AU  - Drobac, Milica
AU  - Omar, Eihab
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5245
AB  - The plants of genus Cymbopogon (family Poaceae) are well known for their use in traditional medicine and for the high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the inflorescences of Cymbopogon nervatus (Hochst.) Chiov. and C. schoenanthus (L.) Spreng. from Sudan were studied. GC-FID/MS analysis showed that C. nervatus essential oils is characterized by the high content of p-menthadienols (64.0-76.2%), whereas oils of C. schoenanthus are rich in monoterpene piperitone (47.7-58.7%). The oils were tested for spasmolytic activity on isolated rat ileum, using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The essential oils of both species exhibited strong, significant and dose-dependent spasmolytic activity. In addition, essential oils were investigated for antimicrobial activity by the microdilution method. Both oils were active against the strains of Staphylococcus aureus (MICs 0.85-1.3 mg/ml), Staphylococcus epidermidis (MICs 0.78-1.3 mg/ml) and Candida albicans (MICs 0.63-1.3 mg/ml), whereas Gram (-) bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) were resistant. The observed spasmolytic effect of the inflorescence essential oils of C. nervatus and C. schoenanthus emphasizes its beneficial properties and is in agreement with their traditional use, especially for gastrointestinal ailments.
C3  - International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual
T1  - Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5245
ER  - 

@conference{
author = "Drobac, Milica and Omar, Eihab and Kovačević, Nada",
year = "2021",
abstract = "The plants of genus Cymbopogon (family Poaceae) are well known for their use in traditional medicine and for the high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the inflorescences of Cymbopogon nervatus (Hochst.) Chiov. and C. schoenanthus (L.) Spreng. from Sudan were studied. GC-FID/MS analysis showed that C. nervatus essential oils is characterized by the high content of p-menthadienols (64.0-76.2%), whereas oils of C. schoenanthus are rich in monoterpene piperitone (47.7-58.7%). The oils were tested for spasmolytic activity on isolated rat ileum, using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The essential oils of both species exhibited strong, significant and dose-dependent spasmolytic activity. In addition, essential oils were investigated for antimicrobial activity by the microdilution method. Both oils were active against the strains of Staphylococcus aureus (MICs 0.85-1.3 mg/ml), Staphylococcus epidermidis (MICs 0.78-1.3 mg/ml) and Candida albicans (MICs 0.63-1.3 mg/ml), whereas Gram (-) bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) were resistant. The observed spasmolytic effect of the inflorescence essential oils of C. nervatus and C. schoenanthus emphasizes its beneficial properties and is in agreement with their traditional use, especially for gastrointestinal ailments.",
journal = "International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual",
title = "Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5245"
}

Drobac, M., Omar, E.,& Kovačević, N.. (2021). Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents. in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual.
https://hdl.handle.net/21.15107/rcub_farfar_5245

Drobac M, Omar E, Kovačević N. Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents. in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5245 .

Drobac, Milica, Omar, Eihab, Kovačević, Nada, "Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents" in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5245 .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Omar, Eihab
AU  - Drobac, Milica
AU  - Radenković, Mirjana
AU  - Branković, Suzana
AU  - Kovačević, Nada
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2831
AB  - The plants of genus Cymbopogon are well known for their use in traditional medicine and for their high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the dried stems and inflorescence of cultivated Cymbopogon schoenanthus (L.) Spreng. (Poaceae) collected from Khartoum state (Sudan) in two periods were studied. The results of chemical analysis of C. schoenanthus revealed that this plant is rich in essential oil which is a valuable source of the commercially important monoterpene, piperitone (47.7-71.5%). Other abundant constituents of the oils were intermedeol (6.1-17.3%), delta-2-carene (4.5-10.0%) and elemol (2.7-9.0%). The essential oil from the inflorescence was tested for spasmolytic activity using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The oil exhibited strong, significant and dose-dependent spasmolytic activity, indicating the possibility for further investigations of this essential oil for its medicinal purposes or application in food industry.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan
VL  - 69
IS  - 3
SP  - 409
EP  - 415
DO  - 10.2298/ABS160506113P
ER  - 

@article{
author = "Pavlović, Ivan and Omar, Eihab and Drobac, Milica and Radenković, Mirjana and Branković, Suzana and Kovačević, Nada",
year = "2017",
abstract = "The plants of genus Cymbopogon are well known for their use in traditional medicine and for their high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the dried stems and inflorescence of cultivated Cymbopogon schoenanthus (L.) Spreng. (Poaceae) collected from Khartoum state (Sudan) in two periods were studied. The results of chemical analysis of C. schoenanthus revealed that this plant is rich in essential oil which is a valuable source of the commercially important monoterpene, piperitone (47.7-71.5%). Other abundant constituents of the oils were intermedeol (6.1-17.3%), delta-2-carene (4.5-10.0%) and elemol (2.7-9.0%). The essential oil from the inflorescence was tested for spasmolytic activity using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The oil exhibited strong, significant and dose-dependent spasmolytic activity, indicating the possibility for further investigations of this essential oil for its medicinal purposes or application in food industry.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan",
volume = "69",
number = "3",
pages = "409-415",
doi = "10.2298/ABS160506113P"
}

Pavlović, I., Omar, E., Drobac, M., Radenković, M., Branković, S.,& Kovačević, N.. (2017). Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(3), 409-415.
https://doi.org/10.2298/ABS160506113P

Pavlović I, Omar E, Drobac M, Radenković M, Branković S, Kovačević N. Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan. in Archives of Biological Sciences. 2017;69(3):409-415.
doi:10.2298/ABS160506113P .

Pavlović, Ivan, Omar, Eihab, Drobac, Milica, Radenković, Mirjana, Branković, Suzana, Kovačević, Nada, "Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan" in Archives of Biological Sciences, 69, no. 3 (2017):409-415,
https://doi.org/10.2298/ABS160506113P . .

TY  - JOUR
AU  - Omar, Eihab
AU  - Pavlović, Ivan
AU  - Drobac, Milica
AU  - Radenković, Mirjana
AU  - Branković, Suzana
AU  - Kovačević, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2741
AB  - The composition of essential oils obtained by hydrodistillation from dried stems and inflorescences of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) growing wild in Western and Eastern Sudan and collected at two different periods, was analyzed by GC and GC MS. The most dominant constituents in the oils were p-menthadienols, trans-p-mentha-1(7),8-dien-2-ol (19.9-32.6%), cis-p-mentha-1(7),8-dien-2-ol (18.9-23.3%), trans-p-mentha-2,8-dien-l-ol (10.6-21.0%) and cis-p-mentha-2,8-dien-1-ol (8.1-10.4%). The essential oil of inflorescence from the Western Sudan was tested for spasmolytic activity using three different experimental models: against spontaneous contractions, and contractions induced with ACh and KCI. The oil exhibited strong, significant and concentration-dependent spasmolytic activity. Applied in concentration of 200 mu g/ml, the oil showed 88.44 +/- 16.78% of maximal spasmolytic effect of atropine (6.4 p,,M) against spontaneous contraction. Essential oil (90 mu g/ml) inhibited ACh-induced contractions as well, and reduced the effect of the highest applied concentration of ACh to 37.29 +/- 16.16%. Additionally, the oil exhibited strong activity against contractions induced with KCI (80 mM) and in concentration of 200 mu g/ml, completely abolished the contractile effect of KCI. The essential oil of inflorescence from the Western Sudan was also tested for antioxidant activity by DPPH assay and showed moderate anti-DPPH activity (SC50 23.8 mu l/ml).
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil
VL  - 91
SP  - 249
EP  - 254
DO  - 10.1016/j.indcrop.2016.07.013
ER  - 

@article{
author = "Omar, Eihab and Pavlović, Ivan and Drobac, Milica and Radenković, Mirjana and Branković, Suzana and Kovačević, Nada",
year = "2016",
abstract = "The composition of essential oils obtained by hydrodistillation from dried stems and inflorescences of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) growing wild in Western and Eastern Sudan and collected at two different periods, was analyzed by GC and GC MS. The most dominant constituents in the oils were p-menthadienols, trans-p-mentha-1(7),8-dien-2-ol (19.9-32.6%), cis-p-mentha-1(7),8-dien-2-ol (18.9-23.3%), trans-p-mentha-2,8-dien-l-ol (10.6-21.0%) and cis-p-mentha-2,8-dien-1-ol (8.1-10.4%). The essential oil of inflorescence from the Western Sudan was tested for spasmolytic activity using three different experimental models: against spontaneous contractions, and contractions induced with ACh and KCI. The oil exhibited strong, significant and concentration-dependent spasmolytic activity. Applied in concentration of 200 mu g/ml, the oil showed 88.44 +/- 16.78% of maximal spasmolytic effect of atropine (6.4 p,,M) against spontaneous contraction. Essential oil (90 mu g/ml) inhibited ACh-induced contractions as well, and reduced the effect of the highest applied concentration of ACh to 37.29 +/- 16.16%. Additionally, the oil exhibited strong activity against contractions induced with KCI (80 mM) and in concentration of 200 mu g/ml, completely abolished the contractile effect of KCI. The essential oil of inflorescence from the Western Sudan was also tested for antioxidant activity by DPPH assay and showed moderate anti-DPPH activity (SC50 23.8 mu l/ml).",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil",
volume = "91",
pages = "249-254",
doi = "10.1016/j.indcrop.2016.07.013"
}

Omar, E., Pavlović, I., Drobac, M., Radenković, M., Branković, S.,& Kovačević, N.. (2016). Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 91, 249-254.
https://doi.org/10.1016/j.indcrop.2016.07.013

Omar E, Pavlović I, Drobac M, Radenković M, Branković S, Kovačević N. Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil. in Industrial Crops and Products. 2016;91:249-254.
doi:10.1016/j.indcrop.2016.07.013 .

Omar, Eihab, Pavlović, Ivan, Drobac, Milica, Radenković, Mirjana, Branković, Suzana, Kovačević, Nada, "Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil" in Industrial Crops and Products, 91 (2016):249-254,
https://doi.org/10.1016/j.indcrop.2016.07.013 . .

TY  - JOUR
AU  - Đukić, Nikola
AU  - Radonjić, Anđa
AU  - Andrić, Goran
AU  - Kljajić, Petar
AU  - Drobac, Milica
AU  - Omar, Eihab
AU  - Kovačević, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2549
AB  - Behavior bioassays were conducted in the laboratory (23 ± 1°C and 50 ± 5% r.h.) using the olfactometer to determine the effects of essential oils of three plant species in the Cymbopogon genus (Lemongrass), Cymbopogon nervatus, C. proximus and C. schoenanthus, on adults of Tribolium castaneum. The effect of essential oils was compared to a commercial biopesticide based on azadirachtin at three concentrations (0.0001, 0.001 and 0.01%). The results showed that all essential oils and the azadirachtin-based biopesticide had significant (p  lt 0.05) repellent effects on T. castaneum adults at all tested concentrations, except C. proximus essential oil which showed a neutral effect at the lowest concentration. The highest concentrations of the essential oils of C. nervatus and C. proximus had significantly stronger repellent effects (p  lt 0.05) than the lowest concentration. At the concentrations of 0.0001 and 0.001% all tested essential oils and azadirachtin showed a similar repellent effect without statistically significant difference, whereas the oil of C. nervatus had the highest repellent effect on adults of T. castaneum at the 0.01% concentration. Considering all tested variations, the essential oils of plants of the genus Cymbopogon showed similar or stronger repellent effects on T. castaneum adults than the biopesticide based on azadirachtin.
AB  - U laboratorijskim uslovima (24±1°C i 50±5% r.v.v.) je pomoću olfaktometra ispitivan uticaj etarskih ulja iz tri biljne vrste roda limunovih trava (Cymbopogon nervatus, Cymbopogon proximus i Cymbopogon schoenanthus) na ponašanje (atraktivnost/repelentnost) adulta T. castaneum. Uticaj etarskih ulja na ponašanje kestenjastog brašnara, poređen je sa uticajem biopesticida na bazi azadirahtina u tri koncentracije: 0.0001, 0.001 i 0.01%. Rezultati testiranja pokazali su da sva etarska ulja i biopesticid na bazi azadirahtina u svim ispitivanim koncentracijama ispoljavaju statistički značajan odbijajući uticaj - repelentni efekat na adulte kestenjastog brašnara, osim najniže koncentracije ulja iz biljke C. proximus koja je ispoljila neutralan efekat na brašnara. Najviša koncentracija ulja iz biljaka C. nervatus i C. proximus je ispoljila statistički značajno jači repelentni efekat u odnosu na najnižu koncentraciju. Sva ispitivana etarska ulja i azadirahtin su u koncentracijama 0.0001 i 0.001% ispoljila sličan repelentni efekat bez statistički značajne razlike, dok je ulje iz biljke C. nervatus u koncentraciji 0.01% prouzrokovalo statistički značajno jači repelentni efekat na adulte kestenjastog brašnara u odnosu na ostale ispitivane supstance. Na osnovu rezultata istraživanja sa adultima kestenjastog brašnara može se zaključiti da su etarska ulja iz tri vrste biljaka iz roda Cymbopogon ispoljila sličan i/ili jači repelentni efekat od biopesticida na bazi azadirahtina. Takođe, dobijeni rezultati pokazuju značajan potencijal upotrebe ulja ovih biljaka kao prirodnog sredstva za zaštitu uskladištenih biljnih proizvoda.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Attractiveness of essential oils of three Cymbopogon species to Tribolium castaneum (Herbst) adults
T1  - Uticaj etarskih uija iz tri vrste biljaka roda Cymbopogon na ponašanje kestenjastog brašnara (Tribolium castaneum Herbst)
VL  - 31
IS  - 3-4
SP  - 129
EP  - 137
DO  - 10.2298/PIF1604129D
ER  - 

@article{
author = "Đukić, Nikola and Radonjić, Anđa and Andrić, Goran and Kljajić, Petar and Drobac, Milica and Omar, Eihab and Kovačević, Nada",
year = "2016",
abstract = "Behavior bioassays were conducted in the laboratory (23 ± 1°C and 50 ± 5% r.h.) using the olfactometer to determine the effects of essential oils of three plant species in the Cymbopogon genus (Lemongrass), Cymbopogon nervatus, C. proximus and C. schoenanthus, on adults of Tribolium castaneum. The effect of essential oils was compared to a commercial biopesticide based on azadirachtin at three concentrations (0.0001, 0.001 and 0.01%). The results showed that all essential oils and the azadirachtin-based biopesticide had significant (p  lt 0.05) repellent effects on T. castaneum adults at all tested concentrations, except C. proximus essential oil which showed a neutral effect at the lowest concentration. The highest concentrations of the essential oils of C. nervatus and C. proximus had significantly stronger repellent effects (p  lt 0.05) than the lowest concentration. At the concentrations of 0.0001 and 0.001% all tested essential oils and azadirachtin showed a similar repellent effect without statistically significant difference, whereas the oil of C. nervatus had the highest repellent effect on adults of T. castaneum at the 0.01% concentration. Considering all tested variations, the essential oils of plants of the genus Cymbopogon showed similar or stronger repellent effects on T. castaneum adults than the biopesticide based on azadirachtin., U laboratorijskim uslovima (24±1°C i 50±5% r.v.v.) je pomoću olfaktometra ispitivan uticaj etarskih ulja iz tri biljne vrste roda limunovih trava (Cymbopogon nervatus, Cymbopogon proximus i Cymbopogon schoenanthus) na ponašanje (atraktivnost/repelentnost) adulta T. castaneum. Uticaj etarskih ulja na ponašanje kestenjastog brašnara, poređen je sa uticajem biopesticida na bazi azadirahtina u tri koncentracije: 0.0001, 0.001 i 0.01%. Rezultati testiranja pokazali su da sva etarska ulja i biopesticid na bazi azadirahtina u svim ispitivanim koncentracijama ispoljavaju statistički značajan odbijajući uticaj - repelentni efekat na adulte kestenjastog brašnara, osim najniže koncentracije ulja iz biljke C. proximus koja je ispoljila neutralan efekat na brašnara. Najviša koncentracija ulja iz biljaka C. nervatus i C. proximus je ispoljila statistički značajno jači repelentni efekat u odnosu na najnižu koncentraciju. Sva ispitivana etarska ulja i azadirahtin su u koncentracijama 0.0001 i 0.001% ispoljila sličan repelentni efekat bez statistički značajne razlike, dok je ulje iz biljke C. nervatus u koncentraciji 0.01% prouzrokovalo statistički značajno jači repelentni efekat na adulte kestenjastog brašnara u odnosu na ostale ispitivane supstance. Na osnovu rezultata istraživanja sa adultima kestenjastog brašnara može se zaključiti da su etarska ulja iz tri vrste biljaka iz roda Cymbopogon ispoljila sličan i/ili jači repelentni efekat od biopesticida na bazi azadirahtina. Takođe, dobijeni rezultati pokazuju značajan potencijal upotrebe ulja ovih biljaka kao prirodnog sredstva za zaštitu uskladištenih biljnih proizvoda.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Attractiveness of essential oils of three Cymbopogon species to Tribolium castaneum (Herbst) adults, Uticaj etarskih uija iz tri vrste biljaka roda Cymbopogon na ponašanje kestenjastog brašnara (Tribolium castaneum Herbst)",
volume = "31",
number = "3-4",
pages = "129-137",
doi = "10.2298/PIF1604129D"
}

Đukić, N., Radonjić, A., Andrić, G., Kljajić, P., Drobac, M., Omar, E.,& Kovačević, N.. (2016). Attractiveness of essential oils of three Cymbopogon species to Tribolium castaneum (Herbst) adults. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 31(3-4), 129-137.
https://doi.org/10.2298/PIF1604129D

Đukić N, Radonjić A, Andrić G, Kljajić P, Drobac M, Omar E, Kovačević N. Attractiveness of essential oils of three Cymbopogon species to Tribolium castaneum (Herbst) adults. in Pesticidi i fitomedicina. 2016;31(3-4):129-137.
doi:10.2298/PIF1604129D .

Đukić, Nikola, Radonjić, Anđa, Andrić, Goran, Kljajić, Petar, Drobac, Milica, Omar, Eihab, Kovačević, Nada, "Attractiveness of essential oils of three Cymbopogon species to Tribolium castaneum (Herbst) adults" in Pesticidi i fitomedicina, 31, no. 3-4 (2016):129-137,
https://doi.org/10.2298/PIF1604129D . .