TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Kuzmanović, Nevena
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5125
AB  - In this work, the overall morpho-anatomical variability of Teucrium montanum sensu lato on the Balkan Peninsula was described. A total of 60 populations were collected all over the Balkans. For each analysed individual, the morphological characters of the stem, leaves, and flowers, as well as the anatomical characters of leaves were measured. The habitats of each population were characterized by altitude zone, type of geological substrate, vegetation, and climate type. Basic descriptive statistics were calculated for each character state, and multivariate analyses were used to compare all assessed characters and groups. The results showed that the analysed populations exhibit high morphological variability and broad ecological preferences. Five of the seven morphologically defined groups fit the already described taxa (T. montanum subsp. montanum, T. montanum subsp. helianthemoides, T. pannonicum, T. skorpili, T. montanum var. parnassicum), whereas two groups are morphologically specific but did not fit the existing taxonomic concepts. The patterns of observed morphological variability only partially match the patterns of ecological differentiation of the habitats of the analysed populations. The occurrence of different morphological groups inhabiting similar habitats in the same biogeographic region suggests that the morpho-anatomical differentiation of T. montanum s.l. may be due to genetic or evolutionary factors.
PB  - Taylor and Francis
T2  - Plant Biosystems
T1  - Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula
VL  - 157
IS  - 3
SP  - 670
EP  - 687
DO  - 10.1080/11263504.2023.2186509
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Branislava and Kuzmanović, Nevena and Stojanović, Danilo and Lakušić, Dmitar",
year = "2023",
abstract = "In this work, the overall morpho-anatomical variability of Teucrium montanum sensu lato on the Balkan Peninsula was described. A total of 60 populations were collected all over the Balkans. For each analysed individual, the morphological characters of the stem, leaves, and flowers, as well as the anatomical characters of leaves were measured. The habitats of each population were characterized by altitude zone, type of geological substrate, vegetation, and climate type. Basic descriptive statistics were calculated for each character state, and multivariate analyses were used to compare all assessed characters and groups. The results showed that the analysed populations exhibit high morphological variability and broad ecological preferences. Five of the seven morphologically defined groups fit the already described taxa (T. montanum subsp. montanum, T. montanum subsp. helianthemoides, T. pannonicum, T. skorpili, T. montanum var. parnassicum), whereas two groups are morphologically specific but did not fit the existing taxonomic concepts. The patterns of observed morphological variability only partially match the patterns of ecological differentiation of the habitats of the analysed populations. The occurrence of different morphological groups inhabiting similar habitats in the same biogeographic region suggests that the morpho-anatomical differentiation of T. montanum s.l. may be due to genetic or evolutionary factors.",
publisher = "Taylor and Francis",
journal = "Plant Biosystems",
title = "Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula",
volume = "157",
number = "3",
pages = "670-687",
doi = "10.1080/11263504.2023.2186509"
}

Zbiljić, M., Lakušić, B., Kuzmanović, N., Stojanović, D.,& Lakušić, D.. (2023). Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula. in Plant Biosystems
Taylor and Francis., 157(3), 670-687.
https://doi.org/10.1080/11263504.2023.2186509

Zbiljić M, Lakušić B, Kuzmanović N, Stojanović D, Lakušić D. Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula. in Plant Biosystems. 2023;157(3):670-687.
doi:10.1080/11263504.2023.2186509 .

Zbiljić, Miloš, Lakušić, Branislava, Kuzmanović, Nevena, Stojanović, Danilo, Lakušić, Dmitar, "Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula" in Plant Biosystems, 157, no. 3 (2023):670-687,
https://doi.org/10.1080/11263504.2023.2186509 . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Kuzmanović, Nevena
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5147
AB  - Teucrium montanum L. is a semi-woody evergreen small shrub distributed in southern and central Europe, Asia Minor, and Algeria in North Africa. The species has a very wide ecological valence occurring in various Mediterranean, sub-Mediterranean, continental as well as oro-Mediterranean and Alpine habitats. Due to its wide ecological valence and broad distribution, T. montanum displays high morphological variability, which is reflected in a large number of described taxa associated with it. In Europe, about thirty taxa have been described at specific and infraspecific levels, of which only T. montanum subsp. montanum and T. montanum subsp. helianthemoides (Adamović) Baden are accepted, while all the others are considered synonyms of T. montanum. The observed morphological diversity is reflected in the presence of six taxa described from the Balkan Peninsula: T. montanum subsp. helianthemoides, T. montanum var. hirsutum Boiss., T. montanum var. modestum Heldr., T. montanum var. parnassicum Čelak., T. pannonicum A. Kern., and T. skorpili Velen. During field research throughout the Balkans, seven morphologically more or less well-defined groups with relatively solid geographical differentiation were observed. Five of the seven groups fit the already described infraspecific taxa, while two groups are morphologically specific but do not correspond to the existing taxa. The aim of this study was to describe the overall morpho-anatomical variability of T. montanum sensu lato in the Balkans and to determine whether the seven identified groups can be distinguished based on morpho-anatomical characters. The most typical populations of these groups could be separated quite well based on the quantitative morphological traits. Multivariate analysis of all samples showed a solid separation of three groups and a significant number of individuals with intermediate positions. Other groups did not show statistical diversification but possess a specific combination of morphological characters by which they can be identified.
PB  - Hrvatsko botaničko društvo (Croatian Botanical Society)
C3  - 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts
T1  - Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula
SP  - 33
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5147
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Kuzmanović, Nevena and Stojanović, Danilo and Lakušić, Dmitar",
year = "2022",
abstract = "Teucrium montanum L. is a semi-woody evergreen small shrub distributed in southern and central Europe, Asia Minor, and Algeria in North Africa. The species has a very wide ecological valence occurring in various Mediterranean, sub-Mediterranean, continental as well as oro-Mediterranean and Alpine habitats. Due to its wide ecological valence and broad distribution, T. montanum displays high morphological variability, which is reflected in a large number of described taxa associated with it. In Europe, about thirty taxa have been described at specific and infraspecific levels, of which only T. montanum subsp. montanum and T. montanum subsp. helianthemoides (Adamović) Baden are accepted, while all the others are considered synonyms of T. montanum. The observed morphological diversity is reflected in the presence of six taxa described from the Balkan Peninsula: T. montanum subsp. helianthemoides, T. montanum var. hirsutum Boiss., T. montanum var. modestum Heldr., T. montanum var. parnassicum Čelak., T. pannonicum A. Kern., and T. skorpili Velen. During field research throughout the Balkans, seven morphologically more or less well-defined groups with relatively solid geographical differentiation were observed. Five of the seven groups fit the already described infraspecific taxa, while two groups are morphologically specific but do not correspond to the existing taxa. The aim of this study was to describe the overall morpho-anatomical variability of T. montanum sensu lato in the Balkans and to determine whether the seven identified groups can be distinguished based on morpho-anatomical characters. The most typical populations of these groups could be separated quite well based on the quantitative morphological traits. Multivariate analysis of all samples showed a solid separation of three groups and a significant number of individuals with intermediate positions. Other groups did not show statistical diversification but possess a specific combination of morphological characters by which they can be identified.",
publisher = "Hrvatsko botaničko društvo (Croatian Botanical Society)",
journal = "7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts",
title = "Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5147"
}

Zbiljić, M., Lakušić, B., Kuzmanović, N., Stojanović, D.,& Lakušić, D.. (2022). Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula. in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts
Hrvatsko botaničko društvo (Croatian Botanical Society)., 33-33.
https://hdl.handle.net/21.15107/rcub_farfar_5147

Zbiljić M, Lakušić B, Kuzmanović N, Stojanović D, Lakušić D. Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula. in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts. 2022;:33-33.
https://hdl.handle.net/21.15107/rcub_farfar_5147 .

Zbiljić, Miloš, Lakušić, Branislava, Kuzmanović, Nevena, Stojanović, Danilo, Lakušić, Dmitar, "Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula" in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts (2022):33-33,
https://hdl.handle.net/21.15107/rcub_farfar_5147 .

TY  - JOUR
AU  - Jovanović, Maja
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
AU  - Zlatković, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3999
AB  - Several related yellow-flowered houseleek species which occur on the Balkan Peninsula are divided into two complexes: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, and S. galicicum) and the S. ruthenicum complex (S. ruthenicum, S. leucanthum, S. kindingeri, and S. zeleborii). Due to strong phenotypic plasticity and a limited number of studies, it is difficult to assert at this point whether all the above species are well defined in the taxonomic sense. Detailed studies of the epidermal structures have not been conducted for any of the species in either complex. The aim of this study was to investigate the degree of variability of the epidermal structures together with their potential usefulness for the taxonomic characterization of the species studied. A total of 18 quantitative characters of the epidermal structures of the adaxial and abaxial surfaces of the rosette leaves were analysed within 16 populations. In all species, the epidermal cells are polygonal or irregularly shaped, with straight or sinuous anticlinal walls, while the rosette leaves are amphistomatic with anisocytic stomata. Simple biseriate multicellular glandular trichomes were found on the adaxial and abaxial surfaces and the margins of the rosette leaves. The results of the descriptive statistics, univariate (ANOVA) and multivariate statistical analysis (CDA, AHC) showed low to high variability in the epidermal cells, guard cells and trichomes. The multivariate analysis showed diversification among the complexes and species. The length of the marginal and apical trichomes of the rosette leaves contributed most to diversification.
AB  - Balkansko poluostrvo nastanjuje nekoliko sličnih, žutocvetnih vrsta čuvarkuća svrstanih u dva kompleksa: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, S. galicicum) i S. ruthenicum (S. ruthenicum, S. leucanthum, S. kindingeri, S. zeleborii). Usled izrazite fenotipske plastičnosti trenutno je teško utvrditi da li su sve vrste pomenutih kompleksa dobro definisane u taksonomskom smislu. Detaljna istraživanja epidermalnih struktura do sada nisu sprovedena ni za jednu od navedenih vrsta. Cilj ove studije bio je da se ispita stepen varijabilnosti epidermalnih struktura i mogućnost njihove primene u taksonomskoj karakterizaciji navedenih vrsta. Ukupno 18 kvantitativnih osobina epidermalnih struktura adaksijalne i abaksijalne površine listova rozete je analizirano na nivou 16 populacija. Kod svih vrsta, epidermalne ćelije su poligonalnog ili nepravilnog oblika, sa ravnim ili vijugavim antiklinalnim zidovima, dok su listovi amfistomatični sa anizocitnim tipom stoma. Prosti biserijatni višećelijski žlezdani trihomi pronađeni su na adaksijalnoj i abaksijalnoj površini, kao i na marginama listova rozete. Rezultati deskriptivne statistike, univarijantnih (ANOVA) i multivarijantnih statističkih analiza (CDA, AHC) ukazuju na nizak do visok stepen varijabilnosti epidermalnih ćelija, ćelija zatvaračica i trihoma. Multivarijantne analize ukazuju na raznolikost kompleksa i vrsta, dok dužine marginalnih i apikalnih trihoma listova u najvećoj meri doprinose njihovoj različitosti.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves
T1  - Različitost žutocvetnih vrsta roda Sempervivum (Crassulaceae) Balkanskog poluostrva: rezultati morfometrijskog istraživanja epidermalnih struktura listova rozete.
VL  - 45
IS  - 2
SP  - 163
EP  - 176
DO  - 10.2298/BOTSERB2102163J
ER  - 

@article{
author = "Jovanović, Maja and Lakušić, Dmitar and Lakušić, Branislava and Zlatković, Bojan",
year = "2021",
abstract = "Several related yellow-flowered houseleek species which occur on the Balkan Peninsula are divided into two complexes: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, and S. galicicum) and the S. ruthenicum complex (S. ruthenicum, S. leucanthum, S. kindingeri, and S. zeleborii). Due to strong phenotypic plasticity and a limited number of studies, it is difficult to assert at this point whether all the above species are well defined in the taxonomic sense. Detailed studies of the epidermal structures have not been conducted for any of the species in either complex. The aim of this study was to investigate the degree of variability of the epidermal structures together with their potential usefulness for the taxonomic characterization of the species studied. A total of 18 quantitative characters of the epidermal structures of the adaxial and abaxial surfaces of the rosette leaves were analysed within 16 populations. In all species, the epidermal cells are polygonal or irregularly shaped, with straight or sinuous anticlinal walls, while the rosette leaves are amphistomatic with anisocytic stomata. Simple biseriate multicellular glandular trichomes were found on the adaxial and abaxial surfaces and the margins of the rosette leaves. The results of the descriptive statistics, univariate (ANOVA) and multivariate statistical analysis (CDA, AHC) showed low to high variability in the epidermal cells, guard cells and trichomes. The multivariate analysis showed diversification among the complexes and species. The length of the marginal and apical trichomes of the rosette leaves contributed most to diversification., Balkansko poluostrvo nastanjuje nekoliko sličnih, žutocvetnih vrsta čuvarkuća svrstanih u dva kompleksa: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, S. galicicum) i S. ruthenicum (S. ruthenicum, S. leucanthum, S. kindingeri, S. zeleborii). Usled izrazite fenotipske plastičnosti trenutno je teško utvrditi da li su sve vrste pomenutih kompleksa dobro definisane u taksonomskom smislu. Detaljna istraživanja epidermalnih struktura do sada nisu sprovedena ni za jednu od navedenih vrsta. Cilj ove studije bio je da se ispita stepen varijabilnosti epidermalnih struktura i mogućnost njihove primene u taksonomskoj karakterizaciji navedenih vrsta. Ukupno 18 kvantitativnih osobina epidermalnih struktura adaksijalne i abaksijalne površine listova rozete je analizirano na nivou 16 populacija. Kod svih vrsta, epidermalne ćelije su poligonalnog ili nepravilnog oblika, sa ravnim ili vijugavim antiklinalnim zidovima, dok su listovi amfistomatični sa anizocitnim tipom stoma. Prosti biserijatni višećelijski žlezdani trihomi pronađeni su na adaksijalnoj i abaksijalnoj površini, kao i na marginama listova rozete. Rezultati deskriptivne statistike, univarijantnih (ANOVA) i multivarijantnih statističkih analiza (CDA, AHC) ukazuju na nizak do visok stepen varijabilnosti epidermalnih ćelija, ćelija zatvaračica i trihoma. Multivarijantne analize ukazuju na raznolikost kompleksa i vrsta, dok dužine marginalnih i apikalnih trihoma listova u najvećoj meri doprinose njihovoj različitosti.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves, Različitost žutocvetnih vrsta roda Sempervivum (Crassulaceae) Balkanskog poluostrva: rezultati morfometrijskog istraživanja epidermalnih struktura listova rozete.",
volume = "45",
number = "2",
pages = "163-176",
doi = "10.2298/BOTSERB2102163J"
}

Jovanović, M., Lakušić, D., Lakušić, B.,& Zlatković, B.. (2021). Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves. in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 45(2), 163-176.
https://doi.org/10.2298/BOTSERB2102163J

Jovanović M, Lakušić D, Lakušić B, Zlatković B. Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves. in Botanica Serbica. 2021;45(2):163-176.
doi:10.2298/BOTSERB2102163J .

Jovanović, Maja, Lakušić, Dmitar, Lakušić, Branislava, Zlatković, Bojan, "Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves" in Botanica Serbica, 45, no. 2 (2021):163-176,
https://doi.org/10.2298/BOTSERB2102163J . .

TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3941
AB  - Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.
PB  - Department of Biology, Faculty of Science, University of Zagreb
T2  - Acta Botanica Croatica
T1  - Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia
VL  - 80
IS  - 1
SP  - 48
EP  - 55
DO  - 10.37427/botcro-2020-033
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2021",
abstract = "Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.",
publisher = "Department of Biology, Faculty of Science, University of Zagreb",
journal = "Acta Botanica Croatica",
title = "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia",
volume = "80",
number = "1",
pages = "48-55",
doi = "10.37427/botcro-2020-033"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2021). Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica
Department of Biology, Faculty of Science, University of Zagreb., 80(1), 48-55.
https://doi.org/10.37427/botcro-2020-033

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica. 2021;80(1):48-55.
doi:10.37427/botcro-2020-033 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia" in Acta Botanica Croatica, 80, no. 1 (2021):48-55,
https://doi.org/10.37427/botcro-2020-033 . .

TY  - JOUR
AU  - Ilić, Milan D.
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan K.
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3533
AB  - Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.
PB  - Wiley-VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)
VL  - 17
IS  - 2
DO  - 10.1002/cbdv.201900544
ER  - 

@article{
author = "Ilić, Milan D. and Marčetić, Mirjana and Zlatković, Bojan K. and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2020",
abstract = "Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.",
publisher = "Wiley-VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)",
volume = "17",
number = "2",
doi = "10.1002/cbdv.201900544"
}

Ilić, M. D., Marčetić, M., Zlatković, B. K., Lakušić, B., Kovačević, N.,& Drobac, M.. (2020). Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity
Wiley-VHCA AG., 17(2).
https://doi.org/10.1002/cbdv.201900544

Ilić MD, Marčetić M, Zlatković BK, Lakušić B, Kovačević N, Drobac M. Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity. 2020;17(2).
doi:10.1002/cbdv.201900544 .

Ilić, Milan D., Marčetić, Mirjana, Zlatković, Bojan K., Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)" in Chemistry and Biodiversity, 17, no. 2 (2020),
https://doi.org/10.1002/cbdv.201900544 . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5304
AB  - Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence
SP  - 16
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5304
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2019",
abstract = "Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence",
pages = "16-16",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5304"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2019). Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):16-16,
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Obradović, Marijana
AU  - Tomić, Emilija
AU  - Teovanović, Anđelka
AU  - Lakušić, Branislava
AU  - Božić, Dragana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5303
AB  - Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria
SP  - 151
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5303
ER  - 

@conference{
author = "Marčetić, Mirjana and Obradović, Marijana and Tomić, Emilija and Teovanović, Anđelka and Lakušić, Branislava and Božić, Dragana",
year = "2019",
abstract = "Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5303"
}

Marčetić, M., Obradović, M., Tomić, E., Teovanović, A., Lakušić, B.,& Božić, D.. (2019). Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303

Marčetić M, Obradović M, Tomić E, Teovanović A, Lakušić B, Božić D. Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

Marčetić, Mirjana, Obradović, Marijana, Tomić, Emilija, Teovanović, Anđelka, Lakušić, Branislava, Božić, Dragana, "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):151-151,
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5293
AB  - This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5293
ER  - 

@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2019",
abstract = "This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5293"
}

Slavkovska, V., Zlatković, B., Lakušić, B., Kovačević, N.,& Drobac, M.. (2019). Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293

Slavkovska V, Zlatković B, Lakušić B, Kovačević N, Drobac M. Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

Slavkovska, Violeta, Zlatković, Bojan, Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):27-27,
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

TY  - CONF
AU  - Ilić, Milan
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Drobac, Milica
AU  - Kovačević, Nada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5256
AB  - The Vlasina plateau shows significant floristic diversity counting 956 species of which 91 may be categorized as endangered
in the flora of Serbia. The genus Geranium is present in the
flora of this region with few species and they are the subject
of our research. The chemical composition of volatile compounds of aerial and underground parts of eight Geranium L.
species (G. macrorrhizum L., G. phaeum L., G. sanguineum L.,
G. robertianum L., G. palustre L., G. pyrenaicum Burm. f., G.
columbinum L. and G. lucidum L.) collected in flowering stage
from Vlasina plateau (Serbia) was examined. The samples of
volatile fraction were obtained by steam distillation and analyzed using GC-FID and GC-MS. The cluster analysis revealed
the separation of volatile compounds of Geranium species to
two clusters. The aerial and underground parts volatiles of G.
macrorrhizum were separated from the other species, based
on the high sequiterpenes content (92.3% and 94.6%), mostly
germacrone (73.3%) in the aerial and δ-guaiene (76.8%) in
the underground parts. The volatile fractions of other species
were mainly composed of sesquiterpenes (10.8-61.8%), diterpenes (12.9-43.0%) and fatty acids and their derivatives (6.6-
21.6%). The exception was the fraction of the underground
parts of G. phaeum which was predominantly made by fatty
acids and their derivatives (76.6%). In the volatile fraction of
G. palustre aerial parts β-selinene (18.6%) and (E)-caryophyllene (15.7%) prevailed. Hexadecanoic acid (15.4%), germacrene D (15.4%), (E)-caryophyllene (10.6%), phytol (10.4%)
and caryophyllene oxide (10.2%) were the most abundant
compounds in volatile fraction of G. pyrenaicum aerial parts,
while phytol (13.2%) and (E)-caryophyllene (9.5%) in the volatile fraction of G. lucidum aerial parts. Hexadecanoic acid
and phytol were dominant compounds in the volatile fraction
of aerial parts of G. sanguineum (21.1% and 17.8%), G. robertianum (12.5% and 19.3%), G. phaeum (7.3% and 41.4%) and
G. columbinum (14.5% and 29.5%). Volatiles of aerial parts of
G. robertianum were also characterized by (E)-caryophylene
(8.0%) and caryophyllene oxide (7.4%), while the volatiles of
G. sanguineum contained β-bisabolene (5.2%). The volatiles
of G. palustre aerial parts have been studied for the first time.
The chemical characterization of volatile compounds of Geranium species may possibly have chemotaxonomic importance
and is significant for further research of Vlasina plateau native
flora.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau
VL  - 42
IS  - 1 supplement
SP  - 141
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5256
ER  - 

@conference{
author = "Ilić, Milan and Marčetić, Mirjana and Zlatković, Bojan and Lakušić, Branislava and Drobac, Milica and Kovačević, Nada",
year = "2018",
abstract = "The Vlasina plateau shows significant floristic diversity counting 956 species of which 91 may be categorized as endangered
in the flora of Serbia. The genus Geranium is present in the
flora of this region with few species and they are the subject
of our research. The chemical composition of volatile compounds of aerial and underground parts of eight Geranium L.
species (G. macrorrhizum L., G. phaeum L., G. sanguineum L.,
G. robertianum L., G. palustre L., G. pyrenaicum Burm. f., G.
columbinum L. and G. lucidum L.) collected in flowering stage
from Vlasina plateau (Serbia) was examined. The samples of
volatile fraction were obtained by steam distillation and analyzed using GC-FID and GC-MS. The cluster analysis revealed
the separation of volatile compounds of Geranium species to
two clusters. The aerial and underground parts volatiles of G.
macrorrhizum were separated from the other species, based
on the high sequiterpenes content (92.3% and 94.6%), mostly
germacrone (73.3%) in the aerial and δ-guaiene (76.8%) in
the underground parts. The volatile fractions of other species
were mainly composed of sesquiterpenes (10.8-61.8%), diterpenes (12.9-43.0%) and fatty acids and their derivatives (6.6-
21.6%). The exception was the fraction of the underground
parts of G. phaeum which was predominantly made by fatty
acids and their derivatives (76.6%). In the volatile fraction of
G. palustre aerial parts β-selinene (18.6%) and (E)-caryophyllene (15.7%) prevailed. Hexadecanoic acid (15.4%), germacrene D (15.4%), (E)-caryophyllene (10.6%), phytol (10.4%)
and caryophyllene oxide (10.2%) were the most abundant
compounds in volatile fraction of G. pyrenaicum aerial parts,
while phytol (13.2%) and (E)-caryophyllene (9.5%) in the volatile fraction of G. lucidum aerial parts. Hexadecanoic acid
and phytol were dominant compounds in the volatile fraction
of aerial parts of G. sanguineum (21.1% and 17.8%), G. robertianum (12.5% and 19.3%), G. phaeum (7.3% and 41.4%) and
G. columbinum (14.5% and 29.5%). Volatiles of aerial parts of
G. robertianum were also characterized by (E)-caryophylene
(8.0%) and caryophyllene oxide (7.4%), while the volatiles of
G. sanguineum contained β-bisabolene (5.2%). The volatiles
of G. palustre aerial parts have been studied for the first time.
The chemical characterization of volatile compounds of Geranium species may possibly have chemotaxonomic importance
and is significant for further research of Vlasina plateau native
flora.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau",
volume = "42",
number = "1 supplement",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5256"
}

Ilić, M., Marčetić, M., Zlatković, B., Lakušić, B., Drobac, M.,& Kovačević, N.. (2018). Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 141-141.
https://hdl.handle.net/21.15107/rcub_farfar_5256

Ilić M, Marčetić M, Zlatković B, Lakušić B, Drobac M, Kovačević N. Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):141-141.
https://hdl.handle.net/21.15107/rcub_farfar_5256 .

Ilić, Milan, Marčetić, Mirjana, Zlatković, Bojan, Lakušić, Branislava, Drobac, Milica, Kovačević, Nada, "Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):141-141,
https://hdl.handle.net/21.15107/rcub_farfar_5256 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Zbiljić, Miloš
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5257
AB  - Teucrium montanum L. is widely distributed in the Balkan
Peninsula, extending from the sea coast to altitudes of over
2100 m a. s. l. with the highest number of occurrences in the
zone between 500 and 1000 m. The aim of the study was to
investigate the chemical composition of essential oil of aerial
parts of T. montanum from 14 different populations from Balkan Peninsula, ten from Serbia (SR-Trešnjica canyon, SR-Brdjanska gorge, SR-Goč, SR-Gornjak gorge, SR-Grza canyon,
SR-Jelašnica, SR-Kopaonik, SR-Maglič, SR-Rtanj, SR-Sićevačka gorge), two from Greece (GR-Olimp, GR-Ossa) and two
from Albania (AL-Deja, AL-Skadar). The essential oils were
obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The
aerial parts of T. montanum contained only small amounts
of oil (traces-0.7%), predominantly composed by sesquiterpene compounds (58.0-99.0%). One population from Albania
(Deja), two from Greece (Olimp, Ossa) and two from Serbia
(Sićevačka gorge, Trešnjica canyon) contained more than 90%
of sesquiterpenes. The composition of essential oils was quite
variable and the main compounds in almost all oils were germacrene D (trace-45.5%), sabinene (trace-23.1%), α-pinene
(trace-20.7%), limonene (trace-20.4%), (E)-caryophyllene (2.9-14.5%), γ-cadinene (trace-13.8%) and δ-cadinene
(trace-12.0%). The cluster analysis revealed the separation
of investigated essential oils to five clusters. The first cluster
(AL-Skadar, SR-Brdjanska gorge, SR-Gornjak gorge, SR-Grza canyon, SR-Goč) is characterized by high germacrene D
content (17.6-45.5%), the second (SR-Maglič, SR-Kopaonik,
SR-Sićevačka gorge) by high sesquiterpene alcohol shyobunol content (14.6-55.2%), the third (AL-Deja, SR-Jelašnica)
by high α-bisabolol content (31.2%-43.7%) and shyobunol
(3.2%) in population AL-Deja, the fourth (SR-Rtanj) by high
cis-sesquisabinene hydrate content (42.4%) and the fifth (GR-Olimp, GR-Ossa, SR-Trešnjica canyon) by high germecrene
D-4-ol (5.96%-61.9%), and β-eudesmol (14.9%) and epi-α-cadinol (10.2%) content in GR-Olimp population. Such a high
variability needs more research to define relationships among
populations.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula
VL  - 42
IS  - 1 supplement
SP  - 133
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5257
ER  - 

@conference{
author = "Marčetić, Mirjana and Zbiljić, Miloš and Lakušić, Dmitar and Lakušić, Branislava",
year = "2018",
abstract = "Teucrium montanum L. is widely distributed in the Balkan
Peninsula, extending from the sea coast to altitudes of over
2100 m a. s. l. with the highest number of occurrences in the
zone between 500 and 1000 m. The aim of the study was to
investigate the chemical composition of essential oil of aerial
parts of T. montanum from 14 different populations from Balkan Peninsula, ten from Serbia (SR-Trešnjica canyon, SR-Brdjanska gorge, SR-Goč, SR-Gornjak gorge, SR-Grza canyon,
SR-Jelašnica, SR-Kopaonik, SR-Maglič, SR-Rtanj, SR-Sićevačka gorge), two from Greece (GR-Olimp, GR-Ossa) and two
from Albania (AL-Deja, AL-Skadar). The essential oils were
obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The
aerial parts of T. montanum contained only small amounts
of oil (traces-0.7%), predominantly composed by sesquiterpene compounds (58.0-99.0%). One population from Albania
(Deja), two from Greece (Olimp, Ossa) and two from Serbia
(Sićevačka gorge, Trešnjica canyon) contained more than 90%
of sesquiterpenes. The composition of essential oils was quite
variable and the main compounds in almost all oils were germacrene D (trace-45.5%), sabinene (trace-23.1%), α-pinene
(trace-20.7%), limonene (trace-20.4%), (E)-caryophyllene (2.9-14.5%), γ-cadinene (trace-13.8%) and δ-cadinene
(trace-12.0%). The cluster analysis revealed the separation
of investigated essential oils to five clusters. The first cluster
(AL-Skadar, SR-Brdjanska gorge, SR-Gornjak gorge, SR-Grza canyon, SR-Goč) is characterized by high germacrene D
content (17.6-45.5%), the second (SR-Maglič, SR-Kopaonik,
SR-Sićevačka gorge) by high sesquiterpene alcohol shyobunol content (14.6-55.2%), the third (AL-Deja, SR-Jelašnica)
by high α-bisabolol content (31.2%-43.7%) and shyobunol
(3.2%) in population AL-Deja, the fourth (SR-Rtanj) by high
cis-sesquisabinene hydrate content (42.4%) and the fifth (GR-Olimp, GR-Ossa, SR-Trešnjica canyon) by high germecrene
D-4-ol (5.96%-61.9%), and β-eudesmol (14.9%) and epi-α-cadinol (10.2%) content in GR-Olimp population. Such a high
variability needs more research to define relationships among
populations.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula",
volume = "42",
number = "1 supplement",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5257"
}

Marčetić, M., Zbiljić, M., Lakušić, D.,& Lakušić, B.. (2018). Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 133-133.
https://hdl.handle.net/21.15107/rcub_farfar_5257

Marčetić M, Zbiljić M, Lakušić D, Lakušić B. Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):133-133.
https://hdl.handle.net/21.15107/rcub_farfar_5257 .

Marčetić, Mirjana, Zbiljić, Miloš, Lakušić, Dmitar, Lakušić, Branislava, "Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):133-133,
https://hdl.handle.net/21.15107/rcub_farfar_5257 .

TY  - CONF
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5254
AB  - Larix decidua L. is a deciduous coniferous native to the mountains of central Europe, in the Alps and Carpathians. The aim
was to investigate anatomy and the chemical composition of
the essential oils of needles, stems and strobilus of L. decidua.
The samples were collected from larch trees from cultivated
conditions (Belgrade, Serbia.) and from natural habitat (Alps,
Austria). Needles of L. decidua, on cross section are more or
less elliptically shaped. Two small secretory canals are in the
mesophyll of the needles, on their lateral sides, just below the
epidermis. The young stem (primary structures) have canals in cortex, some are just below the epidermis and many, that
are in forming phase above the phloem. The older stem (perennial stem) has many secretory canals in the parenchyma of
cortex and in the xylem of secondary wood. The essential oils
were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. Small
amounts of essential oils were extracted from the needles
(0.06-0.41%), stems (0.31-0.54%) and strobilus (0.16 % and
0.19 %) of L. decidua. The monoterpene (28.70-50.40%) and
sesquiterpene compounds (41.80-67.20%) dominated in the
essential oil of needles, monoterpenes (80.50-91.70%) in the
oil of stems, diterpenes (46.50% and 40.50%) and monoterpenes (24.20% and 22.20%) in the oil of strobilus. Main com-
pounds in the essential oil of needles represented germacrene
D (15.2-46.9%), δ-3-carene (4.6-24.6%) and α-pinene (6.3-
14.1%), in the oil of stems α-pinene (12.8-21.8%), δ-3-carene
(8.6-14.3%) and sabinene (0.5-10.3%), while in the oil of strobilus diterpene of abietane type (4.1-10.2%) and nor-abietatetraene (3.9-9.2%) were the most abundant. The larch needles
and stem essential oils from cultivated and natural habitats
were quite similar in qualitative composition. However, the
essential oil of needles from natural habitat contained higher
content of germacrene D and α-pinene and the oil of stem
α-pinene and trans-verbenol. The oil of larch strobilus from
natural habitat was characterized by manool and 3-α-acetoxy-manool, diterpenes that were not present in the oil of
cultivated sample.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)
VL  - 42
IS  - 1 supplement
SP  - 129
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5254
ER  - 

@conference{
author = "Lakušić, Branislava and Marčetić, Mirjana",
year = "2018",
abstract = "Larix decidua L. is a deciduous coniferous native to the mountains of central Europe, in the Alps and Carpathians. The aim
was to investigate anatomy and the chemical composition of
the essential oils of needles, stems and strobilus of L. decidua.
The samples were collected from larch trees from cultivated
conditions (Belgrade, Serbia.) and from natural habitat (Alps,
Austria). Needles of L. decidua, on cross section are more or
less elliptically shaped. Two small secretory canals are in the
mesophyll of the needles, on their lateral sides, just below the
epidermis. The young stem (primary structures) have canals in cortex, some are just below the epidermis and many, that
are in forming phase above the phloem. The older stem (perennial stem) has many secretory canals in the parenchyma of
cortex and in the xylem of secondary wood. The essential oils
were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. Small
amounts of essential oils were extracted from the needles
(0.06-0.41%), stems (0.31-0.54%) and strobilus (0.16 % and
0.19 %) of L. decidua. The monoterpene (28.70-50.40%) and
sesquiterpene compounds (41.80-67.20%) dominated in the
essential oil of needles, monoterpenes (80.50-91.70%) in the
oil of stems, diterpenes (46.50% and 40.50%) and monoterpenes (24.20% and 22.20%) in the oil of strobilus. Main com-
pounds in the essential oil of needles represented germacrene
D (15.2-46.9%), δ-3-carene (4.6-24.6%) and α-pinene (6.3-
14.1%), in the oil of stems α-pinene (12.8-21.8%), δ-3-carene
(8.6-14.3%) and sabinene (0.5-10.3%), while in the oil of strobilus diterpene of abietane type (4.1-10.2%) and nor-abietatetraene (3.9-9.2%) were the most abundant. The larch needles
and stem essential oils from cultivated and natural habitats
were quite similar in qualitative composition. However, the
essential oil of needles from natural habitat contained higher
content of germacrene D and α-pinene and the oil of stem
α-pinene and trans-verbenol. The oil of larch strobilus from
natural habitat was characterized by manool and 3-α-acetoxy-manool, diterpenes that were not present in the oil of
cultivated sample.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)",
volume = "42",
number = "1 supplement",
pages = "129-130",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5254"
}

Lakušić, B.,& Marčetić, M.. (2018). Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae). in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 129-130.
https://hdl.handle.net/21.15107/rcub_farfar_5254

Lakušić B, Marčetić M. Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae). in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):129-130.
https://hdl.handle.net/21.15107/rcub_farfar_5254 .

Lakušić, Branislava, Marčetić, Mirjana, "Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):129-130,
https://hdl.handle.net/21.15107/rcub_farfar_5254 .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
AU  - Lakušić, Branislava
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3053
AB  - Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)
VL  - 42
IS  - 2
SP  - 231
EP  - 239
DO  - 10.5281/zenodo.1468339
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada and Lakušić, Branislava",
year = "2018",
abstract = "Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)",
volume = "42",
number = "2",
pages = "231-239",
doi = "10.5281/zenodo.1468339"
}

Arsenijević, J., Drobac, M., Slavkovska, V., Kovačević, N.,& Lakušić, B.. (2018). Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 42(2), 231-239.
https://doi.org/10.5281/zenodo.1468339

Arsenijević J, Drobac M, Slavkovska V, Kovačević N, Lakušić B. Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica. 2018;42(2):231-239.
doi:10.5281/zenodo.1468339 .

Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, Lakušić, Branislava, "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)" in Botanica Serbica, 42, no. 2 (2018):231-239,
https://doi.org/10.5281/zenodo.1468339 . .

TY  - JOUR
AU  - Jokanović, Dušan
AU  - Vilotić, Dragica
AU  - Nikolić, Vesna D.
AU  - Šijačić-Nikolić, Mirjana
AU  - Lakušić, Branislava
AU  - Jović, Đorđe
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3038
AB  - The paper shows the differences in growth rings width between bald cypress stems grown on two alluvial sites in Serbia - Veliko ratno ostrvo and Backa Palanka. Performing this anatomical research included falling of overall six trunks (3 from one and 3 from another location). After that, appropriate wood cross-sections were made and they served for making permanent anatomical preparations. Obtained results proved a big dependence between radial increment from one side and available water content together with nutrients content on the other side. It caused a very significant correlation between growth rings width and qualitative site features. Bearing on mind this fact, it was established that Veliko ratno ostrvo is more suitable for bald cypress growing. As for behavior of radial increment with ages, there are some differences between these locations, first of all because stems from Veliko ratno ostrvo still did not reach its culmination.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - Growth rings width of bald cypress stems from two alluvial sites in Serbia
VL  - 27
IS  - 1
SP  - 306
EP  - 312
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3038
ER  - 

@article{
author = "Jokanović, Dušan and Vilotić, Dragica and Nikolić, Vesna D. and Šijačić-Nikolić, Mirjana and Lakušić, Branislava and Jović, Đorđe",
year = "2018",
abstract = "The paper shows the differences in growth rings width between bald cypress stems grown on two alluvial sites in Serbia - Veliko ratno ostrvo and Backa Palanka. Performing this anatomical research included falling of overall six trunks (3 from one and 3 from another location). After that, appropriate wood cross-sections were made and they served for making permanent anatomical preparations. Obtained results proved a big dependence between radial increment from one side and available water content together with nutrients content on the other side. It caused a very significant correlation between growth rings width and qualitative site features. Bearing on mind this fact, it was established that Veliko ratno ostrvo is more suitable for bald cypress growing. As for behavior of radial increment with ages, there are some differences between these locations, first of all because stems from Veliko ratno ostrvo still did not reach its culmination.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "Growth rings width of bald cypress stems from two alluvial sites in Serbia",
volume = "27",
number = "1",
pages = "306-312",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3038"
}

Jokanović, D., Vilotić, D., Nikolić, V. D., Šijačić-Nikolić, M., Lakušić, B.,& Jović, Đ.. (2018). Growth rings width of bald cypress stems from two alluvial sites in Serbia. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 27(1), 306-312.
https://hdl.handle.net/21.15107/rcub_farfar_3038

Jokanović D, Vilotić D, Nikolić VD, Šijačić-Nikolić M, Lakušić B, Jović Đ. Growth rings width of bald cypress stems from two alluvial sites in Serbia. in Fresenius Environmental Bulletin. 2018;27(1):306-312.
https://hdl.handle.net/21.15107/rcub_farfar_3038 .

Jokanović, Dušan, Vilotić, Dragica, Nikolić, Vesna D., Šijačić-Nikolić, Mirjana, Lakušić, Branislava, Jović, Đorđe, "Growth rings width of bald cypress stems from two alluvial sites in Serbia" in Fresenius Environmental Bulletin, 27, no. 1 (2018):306-312,
https://hdl.handle.net/21.15107/rcub_farfar_3038 .

TY  - JOUR
AU  - Suručić, Relja V.
AU  - Kundaković, Tatjana
AU  - Lakušić, Branislava
AU  - Drakul, Dragana
AU  - Milovanović, Slobodan R.
AU  - Kovačević, Nada
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3027
AB  - The present paper describes environmental and seasonal-related chemical composition variations, vasorelaxant and angiotensin I-converting enzyme (ACE) activities of essential oil from aerial parts of Seseli pallasii BESSER. The composition was analyzed by GC and GC/MS. Monoterpenes were found to be the most abundant chemical class with alpha-pinene (42.7 - 48.2%) as the most prevalent component. Seseli pallasi essential oil relaxed isolated endothelium-intact mesenteric arteries rings precontracted with phenylephrine with IC50 = 3.10 nl/ml (IC50 = 2.70 mu g/ml). Also, S. pallasii essential oil was found to exhibit a dose-dependent ACE inhibitory activity with an IC50 value of 0.33 mg/ml. In silico evaluation of ACE inhibitory activity of the individual components showed that spathulenol exhibited the best binding affinity with ACE, and the lowest binding energy of -7.5 kcal/mol. The results suggested that combination of vasorelaxing and ACE inhibitory effects of the analyzed S. pallasii essential oil might have the potential therapeutic significance in hypertension.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae)
VL  - 14
IS  - 5
DO  - 10.1002/cbdv.201600407
ER  - 

@article{
author = "Suručić, Relja V. and Kundaković, Tatjana and Lakušić, Branislava and Drakul, Dragana and Milovanović, Slobodan R. and Kovačević, Nada",
year = "2017",
abstract = "The present paper describes environmental and seasonal-related chemical composition variations, vasorelaxant and angiotensin I-converting enzyme (ACE) activities of essential oil from aerial parts of Seseli pallasii BESSER. The composition was analyzed by GC and GC/MS. Monoterpenes were found to be the most abundant chemical class with alpha-pinene (42.7 - 48.2%) as the most prevalent component. Seseli pallasi essential oil relaxed isolated endothelium-intact mesenteric arteries rings precontracted with phenylephrine with IC50 = 3.10 nl/ml (IC50 = 2.70 mu g/ml). Also, S. pallasii essential oil was found to exhibit a dose-dependent ACE inhibitory activity with an IC50 value of 0.33 mg/ml. In silico evaluation of ACE inhibitory activity of the individual components showed that spathulenol exhibited the best binding affinity with ACE, and the lowest binding energy of -7.5 kcal/mol. The results suggested that combination of vasorelaxing and ACE inhibitory effects of the analyzed S. pallasii essential oil might have the potential therapeutic significance in hypertension.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae)",
volume = "14",
number = "5",
doi = "10.1002/cbdv.201600407"
}

Suručić, R. V., Kundaković, T., Lakušić, B., Drakul, D., Milovanović, S. R.,& Kovačević, N.. (2017). Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae). in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 14(5).
https://doi.org/10.1002/cbdv.201600407

Suručić RV, Kundaković T, Lakušić B, Drakul D, Milovanović SR, Kovačević N. Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae). in Chemistry & Biodiversity. 2017;14(5).
doi:10.1002/cbdv.201600407 .

Suručić, Relja V., Kundaković, Tatjana, Lakušić, Branislava, Drakul, Dragana, Milovanović, Slobodan R., Kovačević, Nada, "Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae)" in Chemistry & Biodiversity, 14, no. 5 (2017),
https://doi.org/10.1002/cbdv.201600407 . .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Jančić, Radiša
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3007
AB  - The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Biologia
T1  - Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula
VL  - 72
IS  - 3
SP  - 277
EP  - 291
DO  - 10.1515/biolog-2017-0029
ER  - 

@article{
author = "Slavkovska, Violeta and Lakušić, Branislava and Lakušić, Dmitar and Jančić, Radiša",
year = "2017",
abstract = "The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Biologia",
title = "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula",
volume = "72",
number = "3",
pages = "277-291",
doi = "10.1515/biolog-2017-0029"
}

Slavkovska, V., Lakušić, B., Lakušić, D.,& Jančić, R.. (2017). Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia
Walter de Gruyter Gmbh, Berlin., 72(3), 277-291.
https://doi.org/10.1515/biolog-2017-0029

Slavkovska V, Lakušić B, Lakušić D, Jančić R. Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia. 2017;72(3):277-291.
doi:10.1515/biolog-2017-0029 .

Slavkovska, Violeta, Lakušić, Branislava, Lakušić, Dmitar, Jančić, Radiša, "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula" in Biologia, 72, no. 3 (2017):277-291,
https://doi.org/10.1515/biolog-2017-0029 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2953
AB  - Plant specialised metabolites like essential oils are highly variable depending on genetic and various ecological factors. The aim of the present work was to characterise essential oils of the species Seseli rigidum Waldst. & Kit. (Apiaceae) in various organs on the individual and populational levels. Geographical variability and the impact of climate and soil type on essential oil composition were also investigated. Individually sampled essential oils of roots, aerial parts and fruits of plants from seven populations were analysed by GC-FID and GC-MS. The investigated populations showed high inter-populational and especially intrapopulational variability of essential oil composition. In regard to the variability of essential oils, different chemotypes were defined. The essential oils of S. rigidum roots represented a falcarinol chemotype, oils of aerial parts constituted an alpha-pinene or alpha-pineneisabinene chemotype and fruit essential oils can be characterised as belonging to a complex sabinene/alpha-pinene/beta-phellandrene/falcarinoligermacrene B chemotype. At the species level, analysis of variance (ANOVA), principal component analysis (PCA) and canonical discriminant analysis (CDA) showed that the plant part exerted the strongest influence on the composition of essential oils. Climate had a high impact on composition of the essential oils of roots, aerial parts and fruits, while influence of the substrate was less pronounced. The variations in main compounds of essential oils based on climate or substrate were complex and specific to the plant part.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)
VL  - 135
SP  - 80
EP  - 92
DO  - 10.1016/j.phytochem.2016.12.004
ER  - 

@article{
author = "Marčetić, Mirjana and Kovačević, Nada and Lakušić, Dmitar and Lakušić, Branislava",
year = "2017",
abstract = "Plant specialised metabolites like essential oils are highly variable depending on genetic and various ecological factors. The aim of the present work was to characterise essential oils of the species Seseli rigidum Waldst. & Kit. (Apiaceae) in various organs on the individual and populational levels. Geographical variability and the impact of climate and soil type on essential oil composition were also investigated. Individually sampled essential oils of roots, aerial parts and fruits of plants from seven populations were analysed by GC-FID and GC-MS. The investigated populations showed high inter-populational and especially intrapopulational variability of essential oil composition. In regard to the variability of essential oils, different chemotypes were defined. The essential oils of S. rigidum roots represented a falcarinol chemotype, oils of aerial parts constituted an alpha-pinene or alpha-pineneisabinene chemotype and fruit essential oils can be characterised as belonging to a complex sabinene/alpha-pinene/beta-phellandrene/falcarinoligermacrene B chemotype. At the species level, analysis of variance (ANOVA), principal component analysis (PCA) and canonical discriminant analysis (CDA) showed that the plant part exerted the strongest influence on the composition of essential oils. Climate had a high impact on composition of the essential oils of roots, aerial parts and fruits, while influence of the substrate was less pronounced. The variations in main compounds of essential oils based on climate or substrate were complex and specific to the plant part.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)",
volume = "135",
pages = "80-92",
doi = "10.1016/j.phytochem.2016.12.004"
}

Marčetić, M., Kovačević, N., Lakušić, D.,& Lakušić, B.. (2017). Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae). in Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford., 135, 80-92.
https://doi.org/10.1016/j.phytochem.2016.12.004

Marčetić M, Kovačević N, Lakušić D, Lakušić B. Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae). in Phytochemistry. 2017;135:80-92.
doi:10.1016/j.phytochem.2016.12.004 .

Marčetić, Mirjana, Kovačević, Nada, Lakušić, Dmitar, Lakušić, Branislava, "Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)" in Phytochemistry, 135 (2017):80-92,
https://doi.org/10.1016/j.phytochem.2016.12.004 . .

TY  - JOUR
AU  - Zlatković, Bojan
AU  - Mitić, Zorica S.
AU  - Jovanović, S.
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
AU  - Rajković, J.
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2850
AB  - Sedum album is a succulent plant, with phenotypic variability resulting in delimitation of a number of taxa with diverse taxonomic recognition. We analyzed the taxa from Sedum album in broad sense (S. album, S. micranthum, S. athoum and S. serpentini) from Balkan Peninsula, to check whether the variability of their epidermal structures and epicuticular wax composition corresponds to their taxonomic reliability. Epidermal structures and epicuticular waxes were analyzed using light and scanning electron microscopy, gas chromatography-mass spectrometry and gas chromatography-flame ionization detector chromatography and multivariate statistical techniques. Analyses of studied taxa have shown that significant systematic characters include adaxial epidermal and guard cell length, stomatal frequency and subsidiary cells area on abaxial epidermis. The content of n-alkanes C-29, C-30, C-27, C-32 and C-33 was shown to be valuable criterion for their phytochemical delimitation. The comparison of epicuticular wax microstructures has shown no significant differences. Results indicate presence of two (micromorphology) or three (phytochemistry) well-defined groups of populations, with S. serpentini manifesting a considerable level of separation toward the others. Sedum athoum have shown weak to moderate degree of differentiation, while S. micranthum has shown general resemblance to S. album.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Plant Biosystems
T1  - Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula
VL  - 151
IS  - 6
SP  - 974
EP  - 984
DO  - 10.1080/11263504.2016.1218971
ER  - 

@article{
author = "Zlatković, Bojan and Mitić, Zorica S. and Jovanović, S. and Lakušić, Dmitar and Lakušić, Branislava and Rajković, J. and Stojanović, Gordana",
year = "2017",
abstract = "Sedum album is a succulent plant, with phenotypic variability resulting in delimitation of a number of taxa with diverse taxonomic recognition. We analyzed the taxa from Sedum album in broad sense (S. album, S. micranthum, S. athoum and S. serpentini) from Balkan Peninsula, to check whether the variability of their epidermal structures and epicuticular wax composition corresponds to their taxonomic reliability. Epidermal structures and epicuticular waxes were analyzed using light and scanning electron microscopy, gas chromatography-mass spectrometry and gas chromatography-flame ionization detector chromatography and multivariate statistical techniques. Analyses of studied taxa have shown that significant systematic characters include adaxial epidermal and guard cell length, stomatal frequency and subsidiary cells area on abaxial epidermis. The content of n-alkanes C-29, C-30, C-27, C-32 and C-33 was shown to be valuable criterion for their phytochemical delimitation. The comparison of epicuticular wax microstructures has shown no significant differences. Results indicate presence of two (micromorphology) or three (phytochemistry) well-defined groups of populations, with S. serpentini manifesting a considerable level of separation toward the others. Sedum athoum have shown weak to moderate degree of differentiation, while S. micranthum has shown general resemblance to S. album.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Plant Biosystems",
title = "Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula",
volume = "151",
number = "6",
pages = "974-984",
doi = "10.1080/11263504.2016.1218971"
}

Zlatković, B., Mitić, Z. S., Jovanović, S., Lakušić, D., Lakušić, B., Rajković, J.,& Stojanović, G.. (2017). Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula. in Plant Biosystems
Taylor & Francis Ltd, Abingdon., 151(6), 974-984.
https://doi.org/10.1080/11263504.2016.1218971

Zlatković B, Mitić ZS, Jovanović S, Lakušić D, Lakušić B, Rajković J, Stojanović G. Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula. in Plant Biosystems. 2017;151(6):974-984.
doi:10.1080/11263504.2016.1218971 .

Zlatković, Bojan, Mitić, Zorica S., Jovanović, S., Lakušić, Dmitar, Lakušić, Branislava, Rajković, J., Stojanović, Gordana, "Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula" in Plant Biosystems, 151, no. 6 (2017):974-984,
https://doi.org/10.1080/11263504.2016.1218971 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Suručić, Relja V.
AU  - Kovačević, Nada
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2788
AB  - The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Derdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2-93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para-cymene and lower content of a-pinene.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions
VL  - 82
IS  - 7-8
SP  - 815
EP  - 824
DO  - 10.2298/JSC170320059M
ER  - 

@article{
author = "Marčetić, Mirjana and Suručić, Relja V. and Kovačević, Nada and Lakušić, Dmitar and Lakušić, Branislava",
year = "2017",
abstract = "The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Derdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2-93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para-cymene and lower content of a-pinene.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions",
volume = "82",
number = "7-8",
pages = "815-824",
doi = "10.2298/JSC170320059M"
}

Marčetić, M., Suručić, R. V., Kovačević, N., Lakušić, D.,& Lakušić, B.. (2017). Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(7-8), 815-824.
https://doi.org/10.2298/JSC170320059M

Marčetić M, Suručić RV, Kovačević N, Lakušić D, Lakušić B. Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions. in Journal of the Serbian Chemical Society. 2017;82(7-8):815-824.
doi:10.2298/JSC170320059M .

Marčetić, Mirjana, Suručić, Relja V., Kovačević, Nada, Lakušić, Dmitar, Lakušić, Branislava, "Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions" in Journal of the Serbian Chemical Society, 82, no. 7-8 (2017):815-824,
https://doi.org/10.2298/JSC170320059M . .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Marčetić, Mirjana
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2612
AB  - The chemical composition of the essential oils of different parts of Seseli annuum from Serbia was investigated. beta-Selinene (0-21.4 %), germacrene D (3.4-19.1 %), caryophyllene oxide (1.2-18.1 %), germacrene A (0-14.6%), alpha-selinene (0-12.4 %), beta-elemene (0-11.4 %), E-caryophyllene (3.2-10.3 %), alpha-pinene (0.6-7.5 %), vetiselinenol (0-6.3 %) and isodaucene (0-5.4 %) were dominant compounds in the analysed essential oils. The cluster analysis revealed separation of two groups of essential oils: the first group of oils from fruit, inflorescence and aerial parts in flowering phase with high beta-selinene and alpha-selinene content and the second group of oils from leaf, stem and rosette with higher caryophyllene oxide amounts. The essential oil of rosette was separated from the oils of leaf or stem and contained the highest caryophyllene oxide content while alpha-selinene and beta-selinene were completely absent.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)
VL  - 19
IS  - 3
SP  - 671
EP  - 677
DO  - 10.1080/0972060X.2014.901604
ER  - 

@article{
author = "Kovačević, Nada and Marčetić, Mirjana and Lakušić, Dmitar and Lakušić, Branislava",
year = "2016",
abstract = "The chemical composition of the essential oils of different parts of Seseli annuum from Serbia was investigated. beta-Selinene (0-21.4 %), germacrene D (3.4-19.1 %), caryophyllene oxide (1.2-18.1 %), germacrene A (0-14.6%), alpha-selinene (0-12.4 %), beta-elemene (0-11.4 %), E-caryophyllene (3.2-10.3 %), alpha-pinene (0.6-7.5 %), vetiselinenol (0-6.3 %) and isodaucene (0-5.4 %) were dominant compounds in the analysed essential oils. The cluster analysis revealed separation of two groups of essential oils: the first group of oils from fruit, inflorescence and aerial parts in flowering phase with high beta-selinene and alpha-selinene content and the second group of oils from leaf, stem and rosette with higher caryophyllene oxide amounts. The essential oil of rosette was separated from the oils of leaf or stem and contained the highest caryophyllene oxide content while alpha-selinene and beta-selinene were completely absent.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)",
volume = "19",
number = "3",
pages = "671-677",
doi = "10.1080/0972060X.2014.901604"
}

Kovačević, N., Marčetić, M., Lakušić, D.,& Lakušić, B.. (2016). Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae). in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 19(3), 671-677.
https://doi.org/10.1080/0972060X.2014.901604

Kovačević N, Marčetić M, Lakušić D, Lakušić B. Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae). in Journal of Essential Oil Bearing Plants. 2016;19(3):671-677.
doi:10.1080/0972060X.2014.901604 .

Kovačević, Nada, Marčetić, Mirjana, Lakušić, Dmitar, Lakušić, Branislava, "Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)" in Journal of Essential Oil Bearing Plants, 19, no. 3 (2016):671-677,
https://doi.org/10.1080/0972060X.2014.901604 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2533
AB  - Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson
VL  - 13
IS  - 4
SP  - 427
EP  - 436
DO  - 10.1002/cbdv.201500119
ER  - 

@article{
author = "Marčetić, Mirjana and Milenković, Marina and Lakušić, Dmitar and Lakušić, Branislava",
year = "2016",
abstract = "Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson",
volume = "13",
number = "4",
pages = "427-436",
doi = "10.1002/cbdv.201500119"
}

Marčetić, M., Milenković, M., Lakušić, D.,& Lakušić, B.. (2016). Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 13(4), 427-436.
https://doi.org/10.1002/cbdv.201500119

Marčetić M, Milenković M, Lakušić D, Lakušić B. Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity. 2016;13(4):427-436.
doi:10.1002/cbdv.201500119 .

Marčetić, Mirjana, Milenković, Marina, Lakušić, Dmitar, Lakušić, Branislava, "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson" in Chemistry & Biodiversity, 13, no. 4 (2016):427-436,
https://doi.org/10.1002/cbdv.201500119 . .

TY  - JOUR
AU  - Milenković, Marina
AU  - Božić, Dragana
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2357
AB  - Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) due to the acquisition of resistance to current antimicrobials pose a serious challenge for therapy, and new measures to treat and prevent this infectious pathogen are of crucial importance. Plant essential oils (EOs) and their constituents are promising agents with antimicrobial properties. The aim of this study was to evaluate the antistaphylococcal effect of essential oils from Salvia officinalis using the broth-microdilution method. Essential oils of S. officinalis were isolated from the same individual, but at different life stages - young and old leaves. The effects of combinations of sub-inhibitory concentrations of oil and different antibiotics were evaluated by the checkerboard method. The results, expressed as the fractional inhibitory concentration (FIC) and index (FICI), indicate that the essential oil isolated from young leaves potentiated the inhibitory effect of antibiotics against tested MRSA strains.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus
VL  - 67
IS  - 3
SP  - 949
EP  - 956
DO  - 10.2298/ABS141119057M
ER  - 

@article{
author = "Milenković, Marina and Božić, Dragana and Slavkovska, Violeta and Lakušić, Branislava",
year = "2015",
abstract = "Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) due to the acquisition of resistance to current antimicrobials pose a serious challenge for therapy, and new measures to treat and prevent this infectious pathogen are of crucial importance. Plant essential oils (EOs) and their constituents are promising agents with antimicrobial properties. The aim of this study was to evaluate the antistaphylococcal effect of essential oils from Salvia officinalis using the broth-microdilution method. Essential oils of S. officinalis were isolated from the same individual, but at different life stages - young and old leaves. The effects of combinations of sub-inhibitory concentrations of oil and different antibiotics were evaluated by the checkerboard method. The results, expressed as the fractional inhibitory concentration (FIC) and index (FICI), indicate that the essential oil isolated from young leaves potentiated the inhibitory effect of antibiotics against tested MRSA strains.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus",
volume = "67",
number = "3",
pages = "949-956",
doi = "10.2298/ABS141119057M"
}

Milenković, M., Božić, D., Slavkovska, V.,& Lakušić, B.. (2015). Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 949-956.
https://doi.org/10.2298/ABS141119057M

Milenković M, Božić D, Slavkovska V, Lakušić B. Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus. in Archives of Biological Sciences. 2015;67(3):949-956.
doi:10.2298/ABS141119057M .

Milenković, Marina, Božić, Dragana, Slavkovska, Violeta, Lakušić, Branislava, "Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus" in Archives of Biological Sciences, 67, no. 3 (2015):949-956,
https://doi.org/10.2298/ABS141119057M . .

TY  - CONF
AU  - Milenković, Marina
AU  - Ristić, Mihailo
AU  - Lakušić, Dmitar
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5528
AB  - In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.
PB  - Marmara University Faculty  of Pharmacy
C3  - Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
T1  - Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5528
ER  - 

@conference{
author = "Milenković, Marina and Ristić, Mihailo and Lakušić, Dmitar and Slavkovska, Violeta and Lakušić, Branislava",
year = "2014",
abstract = "In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.",
publisher = "Marmara University Faculty  of Pharmacy",
journal = "Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book",
title = "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5528"
}

Milenković, M., Ristić, M., Lakušić, D., Slavkovska, V.,& Lakušić, B.. (2014). Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
Marmara University Faculty  of Pharmacy., 70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528

Milenković M, Ristić M, Lakušić D, Slavkovska V, Lakušić B. Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book. 2014;:70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

Milenković, Marina, Ristić, Mihailo, Lakušić, Dmitar, Slavkovska, Violeta, Lakušić, Branislava, "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)" in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book (2014):70-70,
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Marčetić, Mirjana
AU  - Slavkovska, Violeta
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2208
AB  - Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)
VL  - 9
IS  - 6
SP  - 859
EP  - 862
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2208
ER  - 

@article{
author = "Lakušić, Branislava and Lakušić, Dmitar and Ristić, Mihailo and Marčetić, Mirjana and Slavkovska, Violeta",
year = "2014",
abstract = "Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)",
volume = "9",
number = "6",
pages = "859-862",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2208"
}

Lakušić, B., Lakušić, D., Ristić, M., Marčetić, M.,& Slavkovska, V.. (2014). Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 9(6), 859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208

Lakušić B, Lakušić D, Ristić M, Marčetić M, Slavkovska V. Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications. 2014;9(6):859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

Lakušić, Branislava, Lakušić, Dmitar, Ristić, Mihailo, Marčetić, Mirjana, Slavkovska, Violeta, "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)" in Natural Product Communications, 9, no. 6 (2014):859-862,
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

TY  - JOUR
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2120
AB  - Inter-population differentiation of the species Teucrium montanum was analyzed on the basis of morpho-anatomical variability of its thirteen populations distributed between 60 and 1750 m above sea level in the Mediterranean and Continental region of the central Balkan Peninsula. Descriptive statistics were performed for each of 28 continuous quantitative characters related to leaf and stem morphology and anatomy. The hypothesis of morphological separation of populations was tested using canonical discriminant analysis (CDA), and discriminant function analysis was used to estimate the contribution of individual characters to overall discrimination. The discrimination between groups in CDA was predominantly based on characteristics of the external structure on the leaf surface, while the internal structure of the leaf and stem did not show such significant differences between the 13 populations. The morpho-anatomical analysis of plants from these populations has confirmed that the species T. montanum belongs to evergreen microphyllic xeromorphic semi-bushes. Statistical analysis revealed four group of populations with significant morphological differentiation. One part of the observed anatomical variability could be explained as an adaptive response to different geographical and recent environmental factors, and another part seemed to be caused by genetic or evolutionary factors.
AB  - Interpopulaciona diferencijacija vrste Teucrium montanum je analizirana na osnovu varijabilnosti morfo- anatomskih karakteristika lista i stabla u okviru 13 populacija koje su rasprostranjene između 60 i 1750 m nadmorske visine, u mediteranskom i kontinentalnom delu Centralnog Balkana (Crna Gora i Srbija). Statističkom analizom je obuhvaćeno 28 kvantitativnih karaktera anatomije i morfologije lista i stabla. Hipoteza o morfo-anatomskoj diferencijaciji populacija testirana je kanonskom diskriminantnom analizom (CDA), dok je diskriminantnom funkcijskom analizom procenjen značaj pojedinačnih karaktera u diferencijaciji populacija. Razdvajanje grupa je pretežno zasnovano na karakterima površinske strukture listova, dok unutrašnja građa lista i stabla nije pokazala tako značajne uticaj na ustanovljene razlike između analiziranih populacija. Na osnovu morfo-anatomskih analiza, utvrđeno je da vrsta T. montanum pripada grupi zimzelenih, mikrofilnih kseromorfnih polužbunova. Statistička analiza je pokazala postojanje četiri grupe populacija čija se varijabilnost, s jedne strane, može tumačiti kao adaptivni odgovor na različite ekološke uslove staništa kao i geografski položaj istraživanih populacija. S druge strane, deo ustanovljene varijabilnosti se izgleda može objasniti i odredjenim genetičkim i evolutivnim faktorima.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula
T1  - Morfo-anatomska diferencijacija vrste teucrium montanum (Lamiaceae) na prostoru Centralnog Balkana
VL  - 38
IS  - 1
SP  - 109
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2120
ER  - 

@article{
author = "Lakušić, Dmitar and Lakušić, Branislava",
year = "2014",
abstract = "Inter-population differentiation of the species Teucrium montanum was analyzed on the basis of morpho-anatomical variability of its thirteen populations distributed between 60 and 1750 m above sea level in the Mediterranean and Continental region of the central Balkan Peninsula. Descriptive statistics were performed for each of 28 continuous quantitative characters related to leaf and stem morphology and anatomy. The hypothesis of morphological separation of populations was tested using canonical discriminant analysis (CDA), and discriminant function analysis was used to estimate the contribution of individual characters to overall discrimination. The discrimination between groups in CDA was predominantly based on characteristics of the external structure on the leaf surface, while the internal structure of the leaf and stem did not show such significant differences between the 13 populations. The morpho-anatomical analysis of plants from these populations has confirmed that the species T. montanum belongs to evergreen microphyllic xeromorphic semi-bushes. Statistical analysis revealed four group of populations with significant morphological differentiation. One part of the observed anatomical variability could be explained as an adaptive response to different geographical and recent environmental factors, and another part seemed to be caused by genetic or evolutionary factors., Interpopulaciona diferencijacija vrste Teucrium montanum je analizirana na osnovu varijabilnosti morfo- anatomskih karakteristika lista i stabla u okviru 13 populacija koje su rasprostranjene između 60 i 1750 m nadmorske visine, u mediteranskom i kontinentalnom delu Centralnog Balkana (Crna Gora i Srbija). Statističkom analizom je obuhvaćeno 28 kvantitativnih karaktera anatomije i morfologije lista i stabla. Hipoteza o morfo-anatomskoj diferencijaciji populacija testirana je kanonskom diskriminantnom analizom (CDA), dok je diskriminantnom funkcijskom analizom procenjen značaj pojedinačnih karaktera u diferencijaciji populacija. Razdvajanje grupa je pretežno zasnovano na karakterima površinske strukture listova, dok unutrašnja građa lista i stabla nije pokazala tako značajne uticaj na ustanovljene razlike između analiziranih populacija. Na osnovu morfo-anatomskih analiza, utvrđeno je da vrsta T. montanum pripada grupi zimzelenih, mikrofilnih kseromorfnih polužbunova. Statistička analiza je pokazala postojanje četiri grupe populacija čija se varijabilnost, s jedne strane, može tumačiti kao adaptivni odgovor na različite ekološke uslove staništa kao i geografski položaj istraživanih populacija. S druge strane, deo ustanovljene varijabilnosti se izgleda može objasniti i odredjenim genetičkim i evolutivnim faktorima.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula, Morfo-anatomska diferencijacija vrste teucrium montanum (Lamiaceae) na prostoru Centralnog Balkana",
volume = "38",
number = "1",
pages = "109-120",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2120"
}

Lakušić, D.,& Lakušić, B.. (2014). Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 38(1), 109-120.
https://hdl.handle.net/21.15107/rcub_farfar_2120

Lakušić D, Lakušić B. Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula. in Botanica Serbica. 2014;38(1):109-120.
https://hdl.handle.net/21.15107/rcub_farfar_2120 .

Lakušić, Dmitar, Lakušić, Branislava, "Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula" in Botanica Serbica, 38, no. 1 (2014):109-120,
https://hdl.handle.net/21.15107/rcub_farfar_2120 .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Lakušić, Branislava
AU  - Kitić, Dušanka
AU  - Milutinović, Milica
AU  - Kostić, Milica
AU  - Miladinović, Bojana
AU  - Kovačević, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2239
AB  - Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium).
AB  - Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)
T1  - Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)
VL  - 31
IS  - 1
SP  - 81
EP  - 85
DO  - 10.2478/afmnai-2014-0009
ER  - 

@article{
author = "Pavlović, Dragana R. and Lakušić, Branislava and Kitić, Dušanka and Milutinović, Milica and Kostić, Milica and Miladinović, Bojana and Kovačević, Nada",
year = "2014",
abstract = "Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium)., Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae), Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)",
volume = "31",
number = "1",
pages = "81-85",
doi = "10.2478/afmnai-2014-0009"
}

Pavlović, D. R., Lakušić, B., Kitić, D., Milutinović, M., Kostić, M., Miladinović, B.,& Kovačević, N.. (2014). Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 31(1), 81-85.
https://doi.org/10.2478/afmnai-2014-0009

Pavlović DR, Lakušić B, Kitić D, Milutinović M, Kostić M, Miladinović B, Kovačević N. Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis. 2014;31(1):81-85.
doi:10.2478/afmnai-2014-0009 .

Pavlović, Dragana R., Lakušić, Branislava, Kitić, Dušanka, Milutinović, Milica, Kostić, Milica, Miladinović, Bojana, Kovačević, Nada, "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)" in Acta Facultatis Medicae Naissensis, 31, no. 1 (2014):81-85,
https://doi.org/10.2478/afmnai-2014-0009 . .