TY  - CONF
AU  - Marčetić, Mirjana
AU  - Ilić, Đorđe
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5141
AB  - Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ...
AB  - The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)
T1  - Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)
SP  - 14
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5141
ER  - 

@conference{
author = "Marčetić, Mirjana and Ilić, Đorđe and Slavkovska, Violeta and Kovačević, Nada",
year = "2023",
abstract = "Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ..., The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae), Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)",
pages = "14-15",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5141"
}

Marčetić, M., Ilić, Đ., Slavkovska, V.,& Kovačević, N.. (2023). Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141

Marčetić M, Ilić Đ, Slavkovska V, Kovačević N. Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141 .

Marčetić, Mirjana, Ilić, Đorđe, Slavkovska, Violeta, Kovačević, Nada, "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):14-15,
https://hdl.handle.net/21.15107/rcub_farfar_5141 .

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Drobac, Milica
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5148
AB  - Satureja montana L. је позната ароматична биљка, чији је садржај и квалитет етарског уља варијабилан и зависи, како од генетичких особина и фазе развића саме биљке, тако и од еколошких услова средине. У овом раду су праћени принос и састав етарског уља изолованог из S.montana која се више година успешно гаји у Башти лековитих и ароматичних биљака Фармацеутског факултета, Универзитета у Београду. ...
AB  - Satureja montana L. is a widely-known aromatic plant, the content and quality of whose essential oil varies and depends on the genetic characteristics and the stage of development of the plant itself and the ecological conditions of the habitat. The present work follows the yield and composition of the essential oil isolated from S. montana sucessfully cultivated for a number of years in the Medical and Aromatic Plant Garden of the Faculty of Pharmacy, University of Belgrade. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Садржај и састав етарског уља врсте Satureja montana L. (Lamiaceae) у различитим фазама развића
T1  - Content and composition of essential oil of Satureja montana L. (Lamiaceae) in different developmental stages
SP  - 15
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5148
ER  - 

@conference{
author = "Slavkovska, Violeta and Drobac, Milica and Arsenijević, Jelena",
year = "2023",
abstract = "Satureja montana L. је позната ароматична биљка, чији је садржај и квалитет етарског уља варијабилан и зависи, како од генетичких особина и фазе развића саме биљке, тако и од еколошких услова средине. У овом раду су праћени принос и састав етарског уља изолованог из S.montana која се више година успешно гаји у Башти лековитих и ароматичних биљака Фармацеутског факултета, Универзитета у Београду. ..., Satureja montana L. is a widely-known aromatic plant, the content and quality of whose essential oil varies and depends on the genetic characteristics and the stage of development of the plant itself and the ecological conditions of the habitat. The present work follows the yield and composition of the essential oil isolated from S. montana sucessfully cultivated for a number of years in the Medical and Aromatic Plant Garden of the Faculty of Pharmacy, University of Belgrade. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Садржај и састав етарског уља врсте Satureja montana L. (Lamiaceae) у различитим фазама развића, Content and composition of essential oil of Satureja montana L. (Lamiaceae) in different developmental stages",
pages = "15-17",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5148"
}

Slavkovska, V., Drobac, M.,& Arsenijević, J.. (2023). Садржај и састав етарског уља врсте Satureja montana L. (Lamiaceae) у различитим фазама развића. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 15-17.
https://hdl.handle.net/21.15107/rcub_farfar_5148

Slavkovska V, Drobac M, Arsenijević J. Садржај и састав етарског уља врсте Satureja montana L. (Lamiaceae) у различитим фазама развића. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:15-17.
https://hdl.handle.net/21.15107/rcub_farfar_5148 .

Slavkovska, Violeta, Drobac, Milica, Arsenijević, Jelena, "Садржај и састав етарског уља врсте Satureja montana L. (Lamiaceae) у различитим фазама развића" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):15-17,
https://hdl.handle.net/21.15107/rcub_farfar_5148 .

TY  - JOUR
AU  - Niketić, Marjan
AU  - Tomović, Gordana
AU  - Anačkov, Goran
AU  - Đorđević, Vladan
AU  - Đurović, Sanja
AU  - Duraki, Šemija
AU  - Kabaš, Eva
AU  - Lakušić, Dmitar
AU  - Petkovski, Gorana
AU  - Petrović, Silvana
AU  - Ranimirović, Milana
AU  - Slavkovska, Violeta
AU  - Ušjak, Ljuboš
AU  - Zbiljić, Miloš
AU  - Zlatković, Bojan
AU  - Stevanović, Vladimir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4958
AB  - This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country.
AB  - У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.
PB  - Natural History Museum, Belgrade, Serbia
T2  - Bulletin of the Natural History Museum
T1  - Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV
T1  - Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV
VL  - 15
SP  - 27
EP  - 96
DO  - 10.5937/bnhmb2215027N
ER  - 

@article{
author = "Niketić, Marjan and Tomović, Gordana and Anačkov, Goran and Đorđević, Vladan and Đurović, Sanja and Duraki, Šemija and Kabaš, Eva and Lakušić, Dmitar and Petkovski, Gorana and Petrović, Silvana and Ranimirović, Milana and Slavkovska, Violeta and Ušjak, Ljuboš and Zbiljić, Miloš and Zlatković, Bojan and Stevanović, Vladimir",
year = "2022",
abstract = "This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country., У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.",
publisher = "Natural History Museum, Belgrade, Serbia",
journal = "Bulletin of the Natural History Museum",
title = "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV, Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV",
volume = "15",
pages = "27-96",
doi = "10.5937/bnhmb2215027N"
}

Niketić, M., Tomović, G., Anačkov, G., Đorđević, V., Đurović, S., Duraki, Š., Kabaš, E., Lakušić, D., Petkovski, G., Petrović, S., Ranimirović, M., Slavkovska, V., Ušjak, L., Zbiljić, M., Zlatković, B.,& Stevanović, V.. (2022). Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum
Natural History Museum, Belgrade, Serbia., 15, 27-96.
https://doi.org/10.5937/bnhmb2215027N

Niketić M, Tomović G, Anačkov G, Đorđević V, Đurović S, Duraki Š, Kabaš E, Lakušić D, Petkovski G, Petrović S, Ranimirović M, Slavkovska V, Ušjak L, Zbiljić M, Zlatković B, Stevanović V. Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum. 2022;15:27-96.
doi:10.5937/bnhmb2215027N .

Niketić, Marjan, Tomović, Gordana, Anačkov, Goran, Đorđević, Vladan, Đurović, Sanja, Duraki, Šemija, Kabaš, Eva, Lakušić, Dmitar, Petkovski, Gorana, Petrović, Silvana, Ranimirović, Milana, Slavkovska, Violeta, Ušjak, Ljuboš, Zbiljić, Miloš, Zlatković, Bojan, Stevanović, Vladimir, "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV" in Bulletin of the Natural History Museum, 15 (2022):27-96,
https://doi.org/10.5937/bnhmb2215027N . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5529
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Univerzitet u Kragujevcu , Agronomski fakultet u Čačku
C3  - XXVII Savetovanje o biotehnologiji 2022, Zbornik radova
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
SP  - 345
EP  - 350
DO  - 10.46793/SBT27.345U
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Univerzitet u Kragujevcu , Agronomski fakultet u Čačku",
journal = "XXVII Savetovanje o biotehnologiji 2022, Zbornik radova",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
pages = "345-350",
doi = "10.46793/SBT27.345U"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova
Univerzitet u Kragujevcu , Agronomski fakultet u Čačku., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Slavkovska, Violeta
AU  - Drobac, Milica
AU  - Stojanović, Danilo
AU  - Zbiljić, Miloš
AU  - Kovačević, Nada
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4621
AB  - Satureja kitaibelii Wierzb. ex Heuff. (Rtanj tea) has distinct morphology and distribution which separate it from highly polymorphic S. montana L. In this work, we investigated phenolic composition of S. kitaibelii and compared it to that of S. montana. Flowering aerial parts of four S. kitaibelii samples were collected in Serbia and Bulgaria and one commercial sample of Rtanj tea was obtained from Serbia. Three samples of S. montana were collected in North Macedonia and Serbia and the fourth one was cultivated sample. Herbs were extracted with 50% ethanol. Dry extract solutions were analyzed by liquid chromatography with UV and MS detection. Rosmarinic acid (RA) and clinopodic acid O (CAO) were quantified using RA as the external standard. All extracts were characterized by presence of phenolic acids, RA (16.5-47.9 mg/g) and CAO (1.6-29.3 mg/g, calculated as RA), and flavonoids. The extracts of S. kitaibelii distinguished by high contents of both RA and CAO, which were present in similar quantities within each extract. On the other hand, extracts of S. montana contained at least 3 times less CAO than RA. The obtained results further confirm distinct properties of S. kitaibelii and give additional arguments to its status as a separate species.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)
SP  - 174
EP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4621
ER  - 

@conference{
author = "Arsenijević, Jelena and Slavkovska, Violeta and Drobac, Milica and Stojanović, Danilo and Zbiljić, Miloš and Kovačević, Nada",
year = "2022",
abstract = "Satureja kitaibelii Wierzb. ex Heuff. (Rtanj tea) has distinct morphology and distribution which separate it from highly polymorphic S. montana L. In this work, we investigated phenolic composition of S. kitaibelii and compared it to that of S. montana. Flowering aerial parts of four S. kitaibelii samples were collected in Serbia and Bulgaria and one commercial sample of Rtanj tea was obtained from Serbia. Three samples of S. montana were collected in North Macedonia and Serbia and the fourth one was cultivated sample. Herbs were extracted with 50% ethanol. Dry extract solutions were analyzed by liquid chromatography with UV and MS detection. Rosmarinic acid (RA) and clinopodic acid O (CAO) were quantified using RA as the external standard. All extracts were characterized by presence of phenolic acids, RA (16.5-47.9 mg/g) and CAO (1.6-29.3 mg/g, calculated as RA), and flavonoids. The extracts of S. kitaibelii distinguished by high contents of both RA and CAO, which were present in similar quantities within each extract. On the other hand, extracts of S. montana contained at least 3 times less CAO than RA. The obtained results further confirm distinct properties of S. kitaibelii and give additional arguments to its status as a separate species.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4621"
}

Arsenijević, J., Slavkovska, V., Drobac, M., Stojanović, D., Zbiljić, M.,& Kovačević, N.. (2022). Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae). in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 174-174.
https://hdl.handle.net/21.15107/rcub_farfar_4621

Arsenijević J, Slavkovska V, Drobac M, Stojanović D, Zbiljić M, Kovačević N. Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae). in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:174-174.
https://hdl.handle.net/21.15107/rcub_farfar_4621 .

Arsenijević, Jelena, Slavkovska, Violeta, Drobac, Milica, Stojanović, Danilo, Zbiljić, Miloš, Kovačević, Nada, "Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):174-174,
https://hdl.handle.net/21.15107/rcub_farfar_4621 .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Dabetić, Nevena
AU  - Marcic, Christophe
AU  - Kovačević, Nada
AU  - Ražić, Slavica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4587
AB  - Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA
oligomers, are important active constituents of Lamiaceae plants. It was found that herb of
Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer
clinopodic acid O (CAO) (1).
In this work, we compared extracting efficiencies of conventional solvents and a deep
eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of
RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication
(room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol
96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C,
120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external
standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and
CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of
CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol
(925 μg/mL). These extracts contained significantly more of RA and CAO than the extract
obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96%
ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10
μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj
tea, for purpose of both quality control and preparation of extracts with potential application
in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and
“green pharmacy” principles.
AB  - Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina
(RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je
ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i
heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona
efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i
limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak
rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60
min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom,
kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1).
Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda.
Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO,
pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je
za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925
μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog
pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane,
ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217
μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke
smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu
ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u
skladu sa principima “zelene hemije” i “zelene farmacije”.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
T1  - Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
VL  - 72
IS  - 4 suplement
SP  - S481
EP  - S482
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4587
ER  - 

@conference{
author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Dabetić, Nevena and Marcic, Christophe and Kovačević, Nada and Ražić, Slavica",
year = "2022",
abstract = "Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA
oligomers, are important active constituents of Lamiaceae plants. It was found that herb of
Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer
clinopodic acid O (CAO) (1).
In this work, we compared extracting efficiencies of conventional solvents and a deep
eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of
RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication
(room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol
96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C,
120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external
standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and
CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of
CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol
(925 μg/mL). These extracts contained significantly more of RA and CAO than the extract
obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96%
ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10
μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj
tea, for purpose of both quality control and preparation of extracts with potential application
in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and
“green pharmacy” principles., Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina
(RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je
ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i
heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona
efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i
limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak
rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60
min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom,
kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1).
Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda.
Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO,
pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je
za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925
μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog
pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane,
ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217
μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke
smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu
ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u
skladu sa principima “zelene hemije” i “zelene farmacije”.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae), Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)",
volume = "72",
number = "4 suplement",
pages = "S481-S482",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4587"
}

Arsenijević, J., Drobac, M., Slavkovska, V., Dabetić, N., Marcic, C., Kovačević, N.,& Ražić, S.. (2022). Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S481-S482.
https://hdl.handle.net/21.15107/rcub_farfar_4587

Arsenijević J, Drobac M, Slavkovska V, Dabetić N, Marcic C, Kovačević N, Ražić S. Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju. 2022;72(4 suplement):S481-S482.
https://hdl.handle.net/21.15107/rcub_farfar_4587 .

Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Dabetić, Nevena, Marcic, Christophe, Kovačević, Nada, Ražić, Slavica, "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S481-S482,
https://hdl.handle.net/21.15107/rcub_farfar_4587 .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4319
AB  - This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far.
AB  - Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)
T1  - Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)
VL  - 46
IS  - 2
SP  - 269
EP  - 284
DO  - 10.2298/BOTSERB2202269S
ER  - 

@article{
author = "Slavkovska, Violeta and Zlatković, Bojan and Marčetić, Mirjana and Kovačević, Nada and Drobac, Milica",
year = "2022",
abstract = "This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far., Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae), Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)",
volume = "46",
number = "2",
pages = "269-284",
doi = "10.2298/BOTSERB2202269S"
}

Slavkovska, V., Zlatković, B., Marčetić, M., Kovačević, N.,& Drobac, M.. (2022). The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 46(2), 269-284.
https://doi.org/10.2298/BOTSERB2202269S

Slavkovska V, Zlatković B, Marčetić M, Kovačević N, Drobac M. The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica. 2022;46(2):269-284.
doi:10.2298/BOTSERB2202269S .

Slavkovska, Violeta, Zlatković, Bojan, Marčetić, Mirjana, Kovačević, Nada, Drobac, Milica, "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)" in Botanica Serbica, 46, no. 2 (2022):269-284,
https://doi.org/10.2298/BOTSERB2202269S . .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Slavkovska, Violeta
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4625
AB  - Introduction
Hyssopus officinalis L. (Lamiaceae) is a perennial aromatic subshrub, distributed mainly in the Mediterranean area. The aerial parts of the plant are used as a culinary and medicinal herb (Hyssopi herba) against various ailments, and its essential oil is used in the perfume industry. It contains essential oil, phenolic acids, and flavonoids. However, in a number of cases, certain differences arose between samples of different origin (Mićović et al., 2019). In this work, we investigated the anatomy and chemical composition of H. officinalis from Mt. Galičica, North Macedonia. Furthermore, based on an a priori knowledge on good antimicrobial activity of essential oils, we investigated its effects against selected microorganisms.
PB  - Macedonian Pharmaceutical Association
PB  - Ss. Cyril and Methodius University in Skopje, Faculty of Pharmacy
C3  - Macedonian Pharmaceutical Bulletin
T1  - Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)
VL  - 68
IS  - 1 suppl
SP  - 421
EP  - 422
DO  - 10.33320/maced.pharm.bull.2022.68.03.203
ER  - 

@conference{
author = "Arsenijević, Jelena and Slavkovska, Violeta and Milenković, Marina",
year = "2022",
abstract = "Introduction
Hyssopus officinalis L. (Lamiaceae) is a perennial aromatic subshrub, distributed mainly in the Mediterranean area. The aerial parts of the plant are used as a culinary and medicinal herb (Hyssopi herba) against various ailments, and its essential oil is used in the perfume industry. It contains essential oil, phenolic acids, and flavonoids. However, in a number of cases, certain differences arose between samples of different origin (Mićović et al., 2019). In this work, we investigated the anatomy and chemical composition of H. officinalis from Mt. Galičica, North Macedonia. Furthermore, based on an a priori knowledge on good antimicrobial activity of essential oils, we investigated its effects against selected microorganisms.",
publisher = "Macedonian Pharmaceutical Association, Ss. Cyril and Methodius University in Skopje, Faculty of Pharmacy",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)",
volume = "68",
number = "1 suppl",
pages = "421-422",
doi = "10.33320/maced.pharm.bull.2022.68.03.203"
}

Arsenijević, J., Slavkovska, V.,& Milenković, M.. (2022). Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae). in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(1 suppl), 421-422.
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.203

Arsenijević J, Slavkovska V, Milenković M. Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae). in Macedonian Pharmaceutical Bulletin. 2022;68(1 suppl):421-422.
doi:10.33320/maced.pharm.bull.2022.68.03.203 .

Arsenijević, Jelena, Slavkovska, Violeta, Milenković, Marina, "Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)" in Macedonian Pharmaceutical Bulletin, 68, no. 1 suppl (2022):421-422,
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.203 . .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Marčetić, Mirjana
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4669
AB  - Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions.
AB  - Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Polyphenolic composition of commercial samples of rtanj tea
T1  - Polifenolni sastav komercijalnih uzoraka rtanjskog čaja
VL  - 71
IS  - 5 suplement
SP  - S54
EP  - S55
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4669
ER  - 

@conference{
author = "Arsenijević, Jelena and Marčetić, Mirjana and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada",
year = "2021",
abstract = "Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions., Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Polyphenolic composition of commercial samples of rtanj tea, Polifenolni sastav komercijalnih uzoraka rtanjskog čaja",
volume = "71",
number = "5 suplement",
pages = "S54-S55",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4669"
}

Arsenijević, J., Marčetić, M., Drobac, M., Slavkovska, V.,& Kovačević, N.. (2021). Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669

Arsenijević J, Marčetić M, Drobac M, Slavkovska V, Kovačević N. Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju. 2021;71(5 suplement):S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669 .

Arsenijević, Jelena, Marčetić, Mirjana, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, "Polyphenolic composition of commercial samples of rtanj tea" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S54-S55,
https://hdl.handle.net/21.15107/rcub_farfar_4669 .

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5293
AB  - This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5293
ER  - 

@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2019",
abstract = "This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5293"
}

Slavkovska, V., Zlatković, B., Lakušić, B., Kovačević, N.,& Drobac, M.. (2019). Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293

Slavkovska V, Zlatković B, Lakušić B, Kovačević N, Drobac M. Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

Slavkovska, Violeta, Zlatković, Bojan, Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):27-27,
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

TY  - JOUR
AU  - Tosić, Svetlana
AU  - Stojicić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3363
AB  - Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer, New York
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
VL  - 249
IS  - 5
SP  - 1365
EP  - 1377
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tosić, Svetlana and Stojicić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer, New York",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
volume = "249",
number = "5",
pages = "1365-1377",
doi = "10.1007/s00425-018-03071-5"
}

Tosić, S., Stojicić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta
Springer, New York., 249(5), 1365-1377.
https://doi.org/10.1007/s00425-018-03071-5

Tosić S, Stojicić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta. 2019;249(5):1365-1377.
doi:10.1007/s00425-018-03071-5 .

Tosić, Svetlana, Stojicić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Planta, 249, no. 5 (2019):1365-1377,
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
AU  - Lakušić, Branislava
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3053
AB  - Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)
VL  - 42
IS  - 2
SP  - 231
EP  - 239
DO  - 10.5281/zenodo.1468339
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada and Lakušić, Branislava",
year = "2018",
abstract = "Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)",
volume = "42",
number = "2",
pages = "231-239",
doi = "10.5281/zenodo.1468339"
}

Arsenijević, J., Drobac, M., Slavkovska, V., Kovačević, N.,& Lakušić, B.. (2018). Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 42(2), 231-239.
https://doi.org/10.5281/zenodo.1468339

Arsenijević J, Drobac M, Slavkovska V, Kovačević N, Lakušić B. Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica. 2018;42(2):231-239.
doi:10.5281/zenodo.1468339 .

Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, Lakušić, Branislava, "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)" in Botanica Serbica, 42, no. 2 (2018):231-239,
https://doi.org/10.5281/zenodo.1468339 . .

TY  - CONF
AU  - Šućur, Jovana
AU  - Malenčić, Đorđe
AU  - Manojlović, Ana
AU  - Prvulović, Dejan
AU  - Slavkovska, Violeta
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5524
AB  - Wild-growing plants are good potential sources of natural substances useful for preventing diseases related to oxidative stress. Th e food industry is becoming increasingly interested
in plants, because of their anti-infl ammatory properties and antioxidant activities. In the present investigation, we described the antioxidant potential of acetone extracts of three aromatic species grown on Kopaonik mountain, Ajuga pyramidalis L., Th ymus praecox Opiz and Vaccinium myrtillus L. by two methods: 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) scavenging and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay and their correlations with total phenolics, tannins, fl avonoids and proanthocyanidins contents. Th e total phenolic and tannins content was determined according to the Folin–Ciocalteu method. Th e total fl avonoids were estimated according to the method described by Markham (1989) and proanthocyanidins according to Sun et al. (1998). For ABTS assay, the procedure followed
the method of Re et al. (1999) with some modifi cations. Th e DPPH assay was done according to the method of Lee et al. (1998). T. praecox extract had the almost twice higher phenolics and tannins content compared to V. myrtillus, which had twice higher total phenolics and tannins content than A. pyramidalis. On the other hand, A. pyramidalis had the highest amount of proanthocyanidins, while the content of total fl avonoids was the highest in V. myrtillus. Th e highest scavenging activity showed T. praecox extract (DPPH: 69,67±0,46 mg trolox/gdw; ABTS: 178,11±0,96 mg trolox/gdw), and the lowest activity showed A. pyramidalis extract. Antioxidant capacity was positively correlated with total phenolics, tannins and flavonoids content. From the obtained results it can be concluded that T. praecox extract had the greatest antioxidant
potential.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Antioxidant potential and polyphenolic content of three medicinal wild-growing species from Kopaonik mountain
VL  - 42
IS  - supplement 1
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5524
ER  - 

@conference{
author = "Šućur, Jovana and Malenčić, Đorđe and Manojlović, Ana and Prvulović, Dejan and Slavkovska, Violeta",
year = "2018",
abstract = "Wild-growing plants are good potential sources of natural substances useful for preventing diseases related to oxidative stress. Th e food industry is becoming increasingly interested
in plants, because of their anti-infl ammatory properties and antioxidant activities. In the present investigation, we described the antioxidant potential of acetone extracts of three aromatic species grown on Kopaonik mountain, Ajuga pyramidalis L., Th ymus praecox Opiz and Vaccinium myrtillus L. by two methods: 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) scavenging and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay and their correlations with total phenolics, tannins, fl avonoids and proanthocyanidins contents. Th e total phenolic and tannins content was determined according to the Folin–Ciocalteu method. Th e total fl avonoids were estimated according to the method described by Markham (1989) and proanthocyanidins according to Sun et al. (1998). For ABTS assay, the procedure followed
the method of Re et al. (1999) with some modifi cations. Th e DPPH assay was done according to the method of Lee et al. (1998). T. praecox extract had the almost twice higher phenolics and tannins content compared to V. myrtillus, which had twice higher total phenolics and tannins content than A. pyramidalis. On the other hand, A. pyramidalis had the highest amount of proanthocyanidins, while the content of total fl avonoids was the highest in V. myrtillus. Th e highest scavenging activity showed T. praecox extract (DPPH: 69,67±0,46 mg trolox/gdw; ABTS: 178,11±0,96 mg trolox/gdw), and the lowest activity showed A. pyramidalis extract. Antioxidant capacity was positively correlated with total phenolics, tannins and flavonoids content. From the obtained results it can be concluded that T. praecox extract had the greatest antioxidant
potential.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Antioxidant potential and polyphenolic content of three medicinal wild-growing species from Kopaonik mountain",
volume = "42",
number = "supplement 1",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5524"
}

Šućur, J., Malenčić, Đ., Manojlović, A., Prvulović, D.,& Slavkovska, V.. (2018). Antioxidant potential and polyphenolic content of three medicinal wild-growing species from Kopaonik mountain. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(supplement 1), 146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5524

Šućur J, Malenčić Đ, Manojlović A, Prvulović D, Slavkovska V. Antioxidant potential and polyphenolic content of three medicinal wild-growing species from Kopaonik mountain. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(supplement 1):146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5524 .

Šućur, Jovana, Malenčić, Đorđe, Manojlović, Ana, Prvulović, Dejan, Slavkovska, Violeta, "Antioxidant potential and polyphenolic content of three medicinal wild-growing species from Kopaonik mountain" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. supplement 1 (2018):146-146,
https://hdl.handle.net/21.15107/rcub_farfar_5524 .

TY  - CONF
AU  - Drobac, Milica
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
AU  - Slavkovska, Violeta
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5285
AB  - Koren vrste Pimpinella saxifraga L. se tradicionalno koristi kao ekspektorans,
bronhosekretolitik i antiflogistik. Cilj ovog rada je ispitivanje anatomske građe, sadržaja
i sastava etarskog ulja vegetativnih organa i ploda P. saxifraga. Biljni materijal je
sakupljan u jugoistočnoj Srbiji: Bojanine vode (Suva planina) i Ostrovica (Sićevačka
klisura). Anatomska analiza izvršena je na trajnim preparatima dobijenim standardnom
metodom pripreme za posmatranje pod svetlosnim mikroskopom. Etarska ulja su
izolovana iz herbe u cvetu, ploda, korena u fazi cvetanja i plodonošenja, destilacijom
vodenom parom i analizirana GC‐FID i GC‐MS metodama.
Rezultati anatomske analize su pokazali da je koren sekundarne građe a stablo
primarne sa zatvorenim kolateralnim sprovodnim snopićima. Listovi su dorziventralni,
hipoamfistomatski, lisna drška je sa lučno raspređenim sprovodnim snopićima, a
plodovi (merikarpi) su polukružnog oblika sa slabo izraženim rebrima, bez trihoma.
Retke, nežlezdane, višećelijske, uniserijatne trihome sa oštrim vrhom su uočene na
stablu, listovima i lisnoj dršci. Sekretorni kanali su brojni u svim organima.
Najviši sadržaj etarskog ulja određen je u plodu (1,48‐1,52%), dok su herba
(0,13‐0,21%) i koren (0,50‐0,53%) sadržali manju količinu ulja (v/m). Etarska ulja istih
organa sa različitih lokaliteta su sličnog kvalitativnog sastava sa manjim kvantitativnim
razlikama. U uljima herbe i ploda dominantna jedinjenja su β‐bisabolen (28,8‐76,0%) i
epoksi‐pseudoizoeugenil‐2‐metilbutirat (7,7‐21,8 %). Najzastupljenija jedinjenja u
uljima korena, u obe faze, su azuleni (31,4‐35,7%) i pregeijeren (13,7‐18,8%). U
ispitivanim etarskim uljima različitih organa P. saxifraga utvrđeno je prisustvo
trinorseskviterpena (azulena i geijerena) i fenilpropanoidnih jedinjenja
pseudoizoeugenil tipa, koja su karakteristični hemijski markeri za etarska ulja biljaka
ovog roda.
AB  - Root of Pimpinella saxifraga L. is traditionally used as expectorant,
bronchosecretolytic and antiphlogistic. The aim was investigation of anatomy, content
and composition of the essential oils of vegetative organs and fruits. Plant material was
collected in South‐Eastern Serbia: Bojanine vode (Mt. Suva planina) and Ostrovica
(Sićevo gorge). The anatomical analysis was conducted on permanent slides obtained
by standard method of preparation. The essential oils obtained by hydrodistillation
from flowering aerial parts, fruits and roots (from flowering and fruiting period) were
analysed by GC‐FID and GC‐MS.
The anatomical analysis revealed a secondary structure of root and primary
structure of stem with closed collateral vascular bundles. The leaves are dorsiventral,
hypoamphistomatic and petiole is with arched vascular bundles. The fruit (mericarp) is
semi‐circular and lightly ribbed in cross section without trichomes. Rare, nonglandular,
multicellular, uniseriate, cuneate trihomes were noted on the stem, leaves
and petiole. Secretory channels are numerous in all organs.
The highest content of essential oil was determined in the fruits (1.48‐1.52%),
while aerial parts (0.13‐0.21%) and roots (0.50‐0.53%) contained a lower amount of oil
(v/w). The oils from the same organs originated from different localities were similar in
qualitative composition with some quantitative differences. The most dominant
constituents in the oils from aerial parts and fruits were β‐bisabolene (28.8‐76.0%) and
epoxy‐pseudoisoeugenyl‐2‐methylbutyrate (7.7‐21.8 %), whereas those of the oils from
roots, in both stages, were azulenes (31.4‐35,7%) and pregeijerene (13.7‐18.8%).
Trinorsesquiterpenes (azulenes and geijerenes) and phenylpropanoid compounds of
the pseudoisoegenyl type, which are characteristic chemical markers of the essential
oils of the plants of this genus, were present in the all analyzed oils.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae)
T1  - Anatomy and chemical analysis of essential oil of Pimpinella saxifraga l. (Apiaceae)
VL  - 68
IS  - 3
SP  - 688
EP  - 689
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5285
ER  - 

@conference{
author = "Drobac, Milica and Zlatković, Bojan and Kovačević, Nada and Slavkovska, Violeta",
year = "2018",
abstract = "Koren vrste Pimpinella saxifraga L. se tradicionalno koristi kao ekspektorans,
bronhosekretolitik i antiflogistik. Cilj ovog rada je ispitivanje anatomske građe, sadržaja
i sastava etarskog ulja vegetativnih organa i ploda P. saxifraga. Biljni materijal je
sakupljan u jugoistočnoj Srbiji: Bojanine vode (Suva planina) i Ostrovica (Sićevačka
klisura). Anatomska analiza izvršena je na trajnim preparatima dobijenim standardnom
metodom pripreme za posmatranje pod svetlosnim mikroskopom. Etarska ulja su
izolovana iz herbe u cvetu, ploda, korena u fazi cvetanja i plodonošenja, destilacijom
vodenom parom i analizirana GC‐FID i GC‐MS metodama.
Rezultati anatomske analize su pokazali da je koren sekundarne građe a stablo
primarne sa zatvorenim kolateralnim sprovodnim snopićima. Listovi su dorziventralni,
hipoamfistomatski, lisna drška je sa lučno raspređenim sprovodnim snopićima, a
plodovi (merikarpi) su polukružnog oblika sa slabo izraženim rebrima, bez trihoma.
Retke, nežlezdane, višećelijske, uniserijatne trihome sa oštrim vrhom su uočene na
stablu, listovima i lisnoj dršci. Sekretorni kanali su brojni u svim organima.
Najviši sadržaj etarskog ulja određen je u plodu (1,48‐1,52%), dok su herba
(0,13‐0,21%) i koren (0,50‐0,53%) sadržali manju količinu ulja (v/m). Etarska ulja istih
organa sa različitih lokaliteta su sličnog kvalitativnog sastava sa manjim kvantitativnim
razlikama. U uljima herbe i ploda dominantna jedinjenja su β‐bisabolen (28,8‐76,0%) i
epoksi‐pseudoizoeugenil‐2‐metilbutirat (7,7‐21,8 %). Najzastupljenija jedinjenja u
uljima korena, u obe faze, su azuleni (31,4‐35,7%) i pregeijeren (13,7‐18,8%). U
ispitivanim etarskim uljima različitih organa P. saxifraga utvrđeno je prisustvo
trinorseskviterpena (azulena i geijerena) i fenilpropanoidnih jedinjenja
pseudoizoeugenil tipa, koja su karakteristični hemijski markeri za etarska ulja biljaka
ovog roda., Root of Pimpinella saxifraga L. is traditionally used as expectorant,
bronchosecretolytic and antiphlogistic. The aim was investigation of anatomy, content
and composition of the essential oils of vegetative organs and fruits. Plant material was
collected in South‐Eastern Serbia: Bojanine vode (Mt. Suva planina) and Ostrovica
(Sićevo gorge). The anatomical analysis was conducted on permanent slides obtained
by standard method of preparation. The essential oils obtained by hydrodistillation
from flowering aerial parts, fruits and roots (from flowering and fruiting period) were
analysed by GC‐FID and GC‐MS.
The anatomical analysis revealed a secondary structure of root and primary
structure of stem with closed collateral vascular bundles. The leaves are dorsiventral,
hypoamphistomatic and petiole is with arched vascular bundles. The fruit (mericarp) is
semi‐circular and lightly ribbed in cross section without trichomes. Rare, nonglandular,
multicellular, uniseriate, cuneate trihomes were noted on the stem, leaves
and petiole. Secretory channels are numerous in all organs.
The highest content of essential oil was determined in the fruits (1.48‐1.52%),
while aerial parts (0.13‐0.21%) and roots (0.50‐0.53%) contained a lower amount of oil
(v/w). The oils from the same organs originated from different localities were similar in
qualitative composition with some quantitative differences. The most dominant
constituents in the oils from aerial parts and fruits were β‐bisabolene (28.8‐76.0%) and
epoxy‐pseudoisoeugenyl‐2‐methylbutyrate (7.7‐21.8 %), whereas those of the oils from
roots, in both stages, were azulenes (31.4‐35,7%) and pregeijerene (13.7‐18.8%).
Trinorsesquiterpenes (azulenes and geijerenes) and phenylpropanoid compounds of
the pseudoisoegenyl type, which are characteristic chemical markers of the essential
oils of the plants of this genus, were present in the all analyzed oils.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae), Anatomy and chemical analysis of essential oil of Pimpinella saxifraga l. (Apiaceae)",
volume = "68",
number = "3",
pages = "688-689",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5285"
}

Drobac, M., Zlatković, B., Kovačević, N.,& Slavkovska, V.. (2018). Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 688-689.
https://hdl.handle.net/21.15107/rcub_farfar_5285

Drobac M, Zlatković B, Kovačević N, Slavkovska V. Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae). in Arhiv za farmaciju. 2018;68(3):688-689.
https://hdl.handle.net/21.15107/rcub_farfar_5285 .

Drobac, Milica, Zlatković, Bojan, Kovačević, Nada, Slavkovska, Violeta, "Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae)" in Arhiv za farmaciju, 68, no. 3 (2018):688-689,
https://hdl.handle.net/21.15107/rcub_farfar_5285 .

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5253
AB  - Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting
dry grasslands, limestone rocky areas and screes
of wide sense Mediterranean area, east European lowlands
and Caucasus. This study explores the anatomical structure,
content and composition of the essential oil of the vegetative
organs and fruit of this species. The plant material was collected
on two localities: the village Izvor (Bosilegrad, Serbia)
and Mt. Galičica (FYR Macedonia). The anatomical studies
were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope.
The essential oils obtained by hydrodistillation from roots
(collected during flowering and fruiting period), aerial parts
with inflorescences, and fruits, were analysed by GC-FID and
GC-MS. The anatomical analysis revealed a secondary structure
of root and primary structure of stem with closed collateral
vascular bundles. Leaves are isobilateral, amphistomatic;
petiole is with arched vascular bundles and the fruit (mericarp)
is semi-circular and lightly ribbed in cross section.
Non-glandular unicellular and bicellular cuneate trichomes
are sparsely distributed on the stem, leaves and petiole, but
very dense on the fruit. Secretory channels are present in all
organs: in the parenchyma of the root and stem cortex, stem
pith, in the phloem of root and vascular bundles of stem and
petiole, by the leaf vascular bundles and in the fruit pericarp.
Essential oil yields from different parts of P. tragium ranged
from 0.2-1.1% (v/w). The main compounds in the oils from
roots (in both stages, from both localities), as well as from
aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes
(trinorsesquiterpenes): pregeijerene (29.0-56.2%)
and gejerene (14.1-22.9%), whereas those from aerial parts
and fruits from village Izvor are β-bisabolene (19.1-57.2%)
and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate
(17.4-22.2%). Trinorsesquiterpenes, which were also
found to be characteristic constituents in some other Pimpinella
species oils, are the most dominant constituents in all
investigated oils, except in the oil of fruit from village Izvor.
In addition, phenylpropanoids of pseudoisoeugenol type are
present in all oils confi rming them as chemical markers of
Pimpinella species analysed so far.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition
VL  - 42
IS  - 1 supplement
SP  - 130
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5253
ER  - 

@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Kovačević, Nada and Drobac, Milica",
year = "2018",
abstract = "Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting
dry grasslands, limestone rocky areas and screes
of wide sense Mediterranean area, east European lowlands
and Caucasus. This study explores the anatomical structure,
content and composition of the essential oil of the vegetative
organs and fruit of this species. The plant material was collected
on two localities: the village Izvor (Bosilegrad, Serbia)
and Mt. Galičica (FYR Macedonia). The anatomical studies
were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope.
The essential oils obtained by hydrodistillation from roots
(collected during flowering and fruiting period), aerial parts
with inflorescences, and fruits, were analysed by GC-FID and
GC-MS. The anatomical analysis revealed a secondary structure
of root and primary structure of stem with closed collateral
vascular bundles. Leaves are isobilateral, amphistomatic;
petiole is with arched vascular bundles and the fruit (mericarp)
is semi-circular and lightly ribbed in cross section.
Non-glandular unicellular and bicellular cuneate trichomes
are sparsely distributed on the stem, leaves and petiole, but
very dense on the fruit. Secretory channels are present in all
organs: in the parenchyma of the root and stem cortex, stem
pith, in the phloem of root and vascular bundles of stem and
petiole, by the leaf vascular bundles and in the fruit pericarp.
Essential oil yields from different parts of P. tragium ranged
from 0.2-1.1% (v/w). The main compounds in the oils from
roots (in both stages, from both localities), as well as from
aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes
(trinorsesquiterpenes): pregeijerene (29.0-56.2%)
and gejerene (14.1-22.9%), whereas those from aerial parts
and fruits from village Izvor are β-bisabolene (19.1-57.2%)
and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate
(17.4-22.2%). Trinorsesquiterpenes, which were also
found to be characteristic constituents in some other Pimpinella
species oils, are the most dominant constituents in all
investigated oils, except in the oil of fruit from village Izvor.
In addition, phenylpropanoids of pseudoisoeugenol type are
present in all oils confi rming them as chemical markers of
Pimpinella species analysed so far.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition",
volume = "42",
number = "1 supplement",
pages = "130-130",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5253"
}

Slavkovska, V., Zlatković, B., Kovačević, N.,& Drobac, M.. (2018). Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 130-130.
https://hdl.handle.net/21.15107/rcub_farfar_5253

Slavkovska V, Zlatković B, Kovačević N, Drobac M. Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):130-130.
https://hdl.handle.net/21.15107/rcub_farfar_5253 .

Slavkovska, Violeta, Zlatković, Bojan, Kovačević, Nada, Drobac, Milica, "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):130-130,
https://hdl.handle.net/21.15107/rcub_farfar_5253 .

TY  - JOUR
AU  - Milenković, Marina
AU  - Stošović, Jelena
AU  - Slavkovska, Violeta
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3146
AB  - The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone (C. sylvatica), pulegone and menthone (C. vardarensis), pulegone and piperitenone (C. nepeta), pulegone, piperitenone, menthone and piperitone (C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 mu g/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI  lt = 0.5) to antagonistic (FICI >= 2) depending on the bacterial strain tested.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics
VL  - 13
IS  - 3
SP  - 371
EP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3146
ER  - 

@article{
author = "Milenković, Marina and Stošović, Jelena and Slavkovska, Violeta",
year = "2018",
abstract = "The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone (C. sylvatica), pulegone and menthone (C. vardarensis), pulegone and piperitenone (C. nepeta), pulegone, piperitenone, menthone and piperitone (C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 mu g/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI  lt = 0.5) to antagonistic (FICI >= 2) depending on the bacterial strain tested.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics",
volume = "13",
number = "3",
pages = "371-374",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3146"
}

Milenković, M., Stošović, J.,& Slavkovska, V.. (2018). Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics. in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 13(3), 371-374.
https://hdl.handle.net/21.15107/rcub_farfar_3146

Milenković M, Stošović J, Slavkovska V. Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics. in Natural Product Communications. 2018;13(3):371-374.
https://hdl.handle.net/21.15107/rcub_farfar_3146 .

Milenković, Marina, Stošović, Jelena, Slavkovska, Violeta, "Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics" in Natural Product Communications, 13, no. 3 (2018):371-374,
https://hdl.handle.net/21.15107/rcub_farfar_3146 .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Jančić, Radiša
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3007
AB  - The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Biologia
T1  - Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula
VL  - 72
IS  - 3
SP  - 277
EP  - 291
DO  - 10.1515/biolog-2017-0029
ER  - 

@article{
author = "Slavkovska, Violeta and Lakušić, Branislava and Lakušić, Dmitar and Jančić, Radiša",
year = "2017",
abstract = "The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Biologia",
title = "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula",
volume = "72",
number = "3",
pages = "277-291",
doi = "10.1515/biolog-2017-0029"
}

Slavkovska, V., Lakušić, B., Lakušić, D.,& Jančić, R.. (2017). Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia
Walter de Gruyter Gmbh, Berlin., 72(3), 277-291.
https://doi.org/10.1515/biolog-2017-0029

Slavkovska V, Lakušić B, Lakušić D, Jančić R. Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia. 2017;72(3):277-291.
doi:10.1515/biolog-2017-0029 .

Slavkovska, Violeta, Lakušić, Branislava, Lakušić, Dmitar, Jančić, Radiša, "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula" in Biologia, 72, no. 3 (2017):277-291,
https://doi.org/10.1515/biolog-2017-0029 . .

TY  - JOUR
AU  - Stojicić, Dragana
AU  - Tosić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2642
AB  - In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N-6-benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 mu M BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.
PB  - Springer, New York
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
VL  - 244
IS  - 2
SP  - 393
EP  - 404
DO  - 10.1007/s00425-016-2513-7
ER  - 

@article{
author = "Stojicić, Dragana and Tosić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N-6-benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 mu M BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.",
publisher = "Springer, New York",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
volume = "244",
number = "2",
pages = "393-404",
doi = "10.1007/s00425-016-2513-7"
}

Stojicić, D., Tosić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Springer, New York., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojicić D, Tosić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojicić, Dragana, Tosić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Milenković, Marina
AU  - Božić, Dragana
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2357
AB  - Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) due to the acquisition of resistance to current antimicrobials pose a serious challenge for therapy, and new measures to treat and prevent this infectious pathogen are of crucial importance. Plant essential oils (EOs) and their constituents are promising agents with antimicrobial properties. The aim of this study was to evaluate the antistaphylococcal effect of essential oils from Salvia officinalis using the broth-microdilution method. Essential oils of S. officinalis were isolated from the same individual, but at different life stages - young and old leaves. The effects of combinations of sub-inhibitory concentrations of oil and different antibiotics were evaluated by the checkerboard method. The results, expressed as the fractional inhibitory concentration (FIC) and index (FICI), indicate that the essential oil isolated from young leaves potentiated the inhibitory effect of antibiotics against tested MRSA strains.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus
VL  - 67
IS  - 3
SP  - 949
EP  - 956
DO  - 10.2298/ABS141119057M
ER  - 

@article{
author = "Milenković, Marina and Božić, Dragana and Slavkovska, Violeta and Lakušić, Branislava",
year = "2015",
abstract = "Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) due to the acquisition of resistance to current antimicrobials pose a serious challenge for therapy, and new measures to treat and prevent this infectious pathogen are of crucial importance. Plant essential oils (EOs) and their constituents are promising agents with antimicrobial properties. The aim of this study was to evaluate the antistaphylococcal effect of essential oils from Salvia officinalis using the broth-microdilution method. Essential oils of S. officinalis were isolated from the same individual, but at different life stages - young and old leaves. The effects of combinations of sub-inhibitory concentrations of oil and different antibiotics were evaluated by the checkerboard method. The results, expressed as the fractional inhibitory concentration (FIC) and index (FICI), indicate that the essential oil isolated from young leaves potentiated the inhibitory effect of antibiotics against tested MRSA strains.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus",
volume = "67",
number = "3",
pages = "949-956",
doi = "10.2298/ABS141119057M"
}

Milenković, M., Božić, D., Slavkovska, V.,& Lakušić, B.. (2015). Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 949-956.
https://doi.org/10.2298/ABS141119057M

Milenković M, Božić D, Slavkovska V, Lakušić B. Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus. in Archives of Biological Sciences. 2015;67(3):949-956.
doi:10.2298/ABS141119057M .

Milenković, Marina, Božić, Dragana, Slavkovska, Violeta, Lakušić, Branislava, "Synergistic effects of Salvia officinalis L. essential oils and antibiotics against methicillin-resistant Staphylococcus aureus" in Archives of Biological Sciences, 67, no. 3 (2015):949-956,
https://doi.org/10.2298/ABS141119057M . .

TY  - CONF
AU  - Milenković, Marina
AU  - Ristić, Mihailo
AU  - Lakušić, Dmitar
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5528
AB  - In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.
PB  - Marmara University Faculty  of Pharmacy
C3  - Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
T1  - Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5528
ER  - 

@conference{
author = "Milenković, Marina and Ristić, Mihailo and Lakušić, Dmitar and Slavkovska, Violeta and Lakušić, Branislava",
year = "2014",
abstract = "In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.",
publisher = "Marmara University Faculty  of Pharmacy",
journal = "Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book",
title = "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5528"
}

Milenković, M., Ristić, M., Lakušić, D., Slavkovska, V.,& Lakušić, B.. (2014). Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
Marmara University Faculty  of Pharmacy., 70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528

Milenković M, Ristić M, Lakušić D, Slavkovska V, Lakušić B. Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book. 2014;:70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

Milenković, Marina, Ristić, Mihailo, Lakušić, Dmitar, Slavkovska, Violeta, Lakušić, Branislava, "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)" in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book (2014):70-70,
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Marčetić, Mirjana
AU  - Slavkovska, Violeta
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2208
AB  - Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)
VL  - 9
IS  - 6
SP  - 859
EP  - 862
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2208
ER  - 

@article{
author = "Lakušić, Branislava and Lakušić, Dmitar and Ristić, Mihailo and Marčetić, Mirjana and Slavkovska, Violeta",
year = "2014",
abstract = "Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)",
volume = "9",
number = "6",
pages = "859-862",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2208"
}

Lakušić, B., Lakušić, D., Ristić, M., Marčetić, M.,& Slavkovska, V.. (2014). Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 9(6), 859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208

Lakušić B, Lakušić D, Ristić M, Marčetić M, Slavkovska V. Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications. 2014;9(6):859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

Lakušić, Branislava, Lakušić, Dmitar, Ristić, Mihailo, Marčetić, Mirjana, Slavkovska, Violeta, "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)" in Natural Product Communications, 9, no. 6 (2014):859-862,
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

TY  - JOUR
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1937
AB  - Seasonal variations in the composition of the essential oils obtained from rosemary plants of the same genotype cultivated in Belgrade were determined by GC and GC/MS. The main constituents were camphor (18.2 - 28.1%), 1,8-cineole (6.4-18.0%), alpha-pinene (9.7-13.5%), borneol (4.4-9.5%), camphene (5.1-8.7%), beta-pinene (2.1-8.1%), beta-phellandrene (4.6-6.5%), myrcene (3.4-5.9%) and bornyl acetate (0.2-7.9%). Cluster analysis showed that 16 samples that had been collected each month during the vegetative cycle can be separated into three main clades with different compositions of essential oils. In the shoots with fruits ('fruits' - Clade I) and shoots with developed leaves ('old shoots' - Clade III) camphor is dominant. In shoots with young and incompletely developed leaves ('young shoot' - Clade II) camphor and 1,8-cineole had almost the same concentration. The fact that the same genotype during the growing seasons can synthesize oils that are so different that they can be classified as different chemotypes confirms the opinion that the chemical composition of essential oils sometimes critically depends on the time of collection. Also, for the definition of chemotypes it is not enough to base this on a chemical analysis of an oil from one phenophase only.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)
VL  - 8
IS  - 1
SP  - 131
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1937
ER  - 

@article{
author = "Lakušić, Dmitar and Ristić, Mihailo and Slavkovska, Violeta and Lakušić, Branislava",
year = "2013",
abstract = "Seasonal variations in the composition of the essential oils obtained from rosemary plants of the same genotype cultivated in Belgrade were determined by GC and GC/MS. The main constituents were camphor (18.2 - 28.1%), 1,8-cineole (6.4-18.0%), alpha-pinene (9.7-13.5%), borneol (4.4-9.5%), camphene (5.1-8.7%), beta-pinene (2.1-8.1%), beta-phellandrene (4.6-6.5%), myrcene (3.4-5.9%) and bornyl acetate (0.2-7.9%). Cluster analysis showed that 16 samples that had been collected each month during the vegetative cycle can be separated into three main clades with different compositions of essential oils. In the shoots with fruits ('fruits' - Clade I) and shoots with developed leaves ('old shoots' - Clade III) camphor is dominant. In shoots with young and incompletely developed leaves ('young shoot' - Clade II) camphor and 1,8-cineole had almost the same concentration. The fact that the same genotype during the growing seasons can synthesize oils that are so different that they can be classified as different chemotypes confirms the opinion that the chemical composition of essential oils sometimes critically depends on the time of collection. Also, for the definition of chemotypes it is not enough to base this on a chemical analysis of an oil from one phenophase only.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)",
volume = "8",
number = "1",
pages = "131-134",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1937"
}

Lakušić, D., Ristić, M., Slavkovska, V.,& Lakušić, B.. (2013). Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae). in Natural Product Communications
Natural Products Inc, Westerville., 8(1), 131-134.
https://hdl.handle.net/21.15107/rcub_farfar_1937

Lakušić D, Ristić M, Slavkovska V, Lakušić B. Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae). in Natural Product Communications. 2013;8(1):131-134.
https://hdl.handle.net/21.15107/rcub_farfar_1937 .

Lakušić, Dmitar, Ristić, Mihailo, Slavkovska, Violeta, Lakušić, Branislava, "Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)" in Natural Product Communications, 8, no. 1 (2013):131-134,
https://hdl.handle.net/21.15107/rcub_farfar_1937 .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1880
AB  - Variations in the yield and composition of essential oil of Dalmatian sage (Salvia officinalis L., Lamiaceae) in various stages of development were analyzed in two individuals (= genotypes) of different geographic origin. Both plants have been successfully grown ten years under identical environmental conditions in a private garden in Belgrade. The amount and composition of the oils were analyzed during one growing season starting from the young to old overwintered leaves. The results of the cluster analysis showed that leaf age and origin of the plants has a significant impact on the composition of the essential oils. All oil samples formed two main clades. The first clade are oils collected from young leaves from April to June and belong to α-humulene type. The second clade includes oil obtained mainly of old leaves, which were collected from August to June, and belonging to the camphor and thujone type. In the second clade samples originating from Učka are completely separated from samples originating from Belgrade. Also, based on these analyzes showed that the same individual (the same genotype) during one growing season significantly change the chemical composition of the essential oils. Thus, it is possible to distinguish three different 'phenological types of essential oils': young leaves ('yl-oils'), early old leaves ('early-ol-oils') and a late old leaves type ('late-ol-oils'). Analysis of the seasonal changes in the composition of the essential oils of Dalmatian sage is shown that different components have different dynamics and different directions of change in the concentration during the season.
AB  - Variranje prinosa i sastava etarskog ulja dalmatinske žalfije (Salvia officinalis L., Lamiaceae) u različitim fazama razvoja analizirano je na dve individue (= genotipa) različitog geografskog porekla. Obe biljke su uspešno gajene deset godina pod identičnom ekološkim uslovima u privatnoj bašti u Beogradu. Količina i sastav ulja su analizirani tokom jedne vegetacione sezone počevši od mladih pa sve do starih prezimelih listova. Rezultati klaster analize su pokazali da starost listova kao i poreklo biljke ima veoma značajan uticaj na sastav etarskog ulja. Svi uzorci ulja su formirali dve osnovne klade. Prvu kladu čine ulja mladih listova sakupljanih od aprila do juna i pripadaju α-humulen tipu. Druga klada obuhvata ulja dobijena uglavnom od starih listova, koji su sakupljani od avgusta do juna, i koji pripadaju kamfor ili tujon tipu. U drugoj kladi uzorci poreklom sa Učke su potpuno odvojeni od uzoraka poreklom iz Beograda. Takođe, na osnovu ovih analiza je utvrđeno da ista individua (isti genotip) u toku jedne vegetacione sezone značajno menja hemijski sastav etarskog ulja. Tako je moguće razlikovati tri različita 'fenološka tipa etarskih ulja': ulja mladih listova, ulja rane faze starih listova i ulja kasne faze starih listova. Analiza sezonskih promena u sastavu etarskih ulja Dalmatinske žalfije je pokazala da različite komponente imaju različitu dinamiku i različite pravce promena u koncentraciji tokom sezone.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages
T1  - Variranje prinosa i sastava etarskih ulja dalmatinske žalfije (Salvia officinalis Lamiaceae) u različitim fazama razvoja
VL  - 37
IS  - 2
SP  - 127
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1880
ER  - 

@article{
author = "Lakušić, Branislava and Ristić, Mihailo and Slavkovska, Violeta and Stojanović, Danilo and Lakušić, Dmitar",
year = "2013",
abstract = "Variations in the yield and composition of essential oil of Dalmatian sage (Salvia officinalis L., Lamiaceae) in various stages of development were analyzed in two individuals (= genotypes) of different geographic origin. Both plants have been successfully grown ten years under identical environmental conditions in a private garden in Belgrade. The amount and composition of the oils were analyzed during one growing season starting from the young to old overwintered leaves. The results of the cluster analysis showed that leaf age and origin of the plants has a significant impact on the composition of the essential oils. All oil samples formed two main clades. The first clade are oils collected from young leaves from April to June and belong to α-humulene type. The second clade includes oil obtained mainly of old leaves, which were collected from August to June, and belonging to the camphor and thujone type. In the second clade samples originating from Učka are completely separated from samples originating from Belgrade. Also, based on these analyzes showed that the same individual (the same genotype) during one growing season significantly change the chemical composition of the essential oils. Thus, it is possible to distinguish three different 'phenological types of essential oils': young leaves ('yl-oils'), early old leaves ('early-ol-oils') and a late old leaves type ('late-ol-oils'). Analysis of the seasonal changes in the composition of the essential oils of Dalmatian sage is shown that different components have different dynamics and different directions of change in the concentration during the season., Variranje prinosa i sastava etarskog ulja dalmatinske žalfije (Salvia officinalis L., Lamiaceae) u različitim fazama razvoja analizirano je na dve individue (= genotipa) različitog geografskog porekla. Obe biljke su uspešno gajene deset godina pod identičnom ekološkim uslovima u privatnoj bašti u Beogradu. Količina i sastav ulja su analizirani tokom jedne vegetacione sezone počevši od mladih pa sve do starih prezimelih listova. Rezultati klaster analize su pokazali da starost listova kao i poreklo biljke ima veoma značajan uticaj na sastav etarskog ulja. Svi uzorci ulja su formirali dve osnovne klade. Prvu kladu čine ulja mladih listova sakupljanih od aprila do juna i pripadaju α-humulen tipu. Druga klada obuhvata ulja dobijena uglavnom od starih listova, koji su sakupljani od avgusta do juna, i koji pripadaju kamfor ili tujon tipu. U drugoj kladi uzorci poreklom sa Učke su potpuno odvojeni od uzoraka poreklom iz Beograda. Takođe, na osnovu ovih analiza je utvrđeno da ista individua (isti genotip) u toku jedne vegetacione sezone značajno menja hemijski sastav etarskog ulja. Tako je moguće razlikovati tri različita 'fenološka tipa etarskih ulja': ulja mladih listova, ulja rane faze starih listova i ulja kasne faze starih listova. Analiza sezonskih promena u sastavu etarskih ulja Dalmatinske žalfije je pokazala da različite komponente imaju različitu dinamiku i različite pravce promena u koncentraciji tokom sezone.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages, Variranje prinosa i sastava etarskih ulja dalmatinske žalfije (Salvia officinalis Lamiaceae) u različitim fazama razvoja",
volume = "37",
number = "2",
pages = "127-139",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1880"
}

Lakušić, B., Ristić, M., Slavkovska, V., Stojanović, D.,& Lakušić, D.. (2013). Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 37(2), 127-139.
https://hdl.handle.net/21.15107/rcub_farfar_1880

Lakušić B, Ristić M, Slavkovska V, Stojanović D, Lakušić D. Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. in Botanica Serbica. 2013;37(2):127-139.
https://hdl.handle.net/21.15107/rcub_farfar_1880 .

Lakušić, Branislava, Ristić, Mihailo, Slavkovska, Violeta, Stojanović, Danilo, Lakušić, Dmitar, "Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages" in Botanica Serbica, 37, no. 2 (2013):127-139,
https://hdl.handle.net/21.15107/rcub_farfar_1880 .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Bräuchler, Christian
AU  - Stojanović, Danilo
AU  - Tzakou, Olga
AU  - Couladis, Maria
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1879
AB  - The variability of essential oil characteristics in different phenological stages of Clinopodium pulegium from its natural habitat (Svrljiški Timok gorge, Serbia) and from cultivated plants (Niš, Serbia) was determined. The essential oils were obtained from aerial parts of the plants by hydrodistillation and analyzed by GC-MS. These are the first data on the essential oil characteristics of plants of the population from the Svrljiški Timok gorge. Samples originating from both natural and cultivated populations were characterized by high amounts of essential oils in all stages of development (0.8% - 1.4%). Twenty-one compounds were identified, representing 95.3-99.6% of the total oils. Differences in the essential oil composition were more quantitative than qualitative. The quality of the essential oil was stable and did not vary with differences in environmental conditions. Dominance of the main components was modified by phenological stage. Pulegone was dominant in the vegetative (76.1% wild population, 62.7% cultivated population) and the flowering (49.5%, 64.6%) stages, while menthone (48.5%, 65.3%) displaced pulegone (34.7%, 18.4%) at the fruiting stage. Cultivated plants in the vegetative and flowering stages can be considered a significant source of pulegone, and in the fruiting stage a significant source of menthone. Careful selection of the developmental stage of the plant is a potential tool which could be employed to obtain the preferred chemical composition of C. pulegium for commercial use.
AB  - U radu je praćena promenljivost količine i sastava etarkog ulja Clinopodium pulegium kroz različite stadijume razvoja biljke (vegetativni, stadijum cvetanja i plodonošenja). Materijal je sakupljan iz samonikle (klisura Svrljiškog Timoka) i gajene (Niš) populacije. Etarsko ulje je izolovano iz nadzemnih delova biljaka destilacijom vodenom parom i analizirano GC-MS metodom. Ovo su prvi podaci o osobinama etarskog ulja biljaka populacije iz klisure Svrljiškog Timoka. Svi uzorci kako iz samonikle tako i iz gajene populacije su se odlikovali velikim sadržajem ulja (0.8% - 1.4%) u svim stadijumima razvoja. Dvadeset jedna komponenta je identifikovana što predstavlja 95.3-99.6% ukupnog etarskog ulja. Razlike u sastavu ulja su bile više kvantitativne nego kvalitativne. Kvalitet etarskog ulja je bio stabilan a variranja uslovljena promenom sredine relativno mala. Dominacija glavnih komponenata je bila uslovljena promenama fenoloških faza. Pulegon je bio dominantan u vegetativnoj (76.1% u ulju biljaka samonikle populacije, 62.7% u ulju biljaka gajene populacije) i fazi cvetanja (49.5%, 64.6%), dok je menton bio dominantan (48.5%, 65.3%) u fazi plodonošenja. Rezultati su pokazali da se biljke u vegetativnoj i fazi cvetanja mogu smatrati značajnim izvorom pulegona, a u fazi plodonošenja značajnim izvorom mentona. Pažljivim odabirom razvojnog stadijuma biljke moguće je dobiti etarsko ulje željenog sastava koje bi se moglo upotrebiti u komercijalne svrhe.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage
T1  - Promenljivost osobina etarskog ulja vrste Clinopodium pulegium (Lamiaceae) u zavisnosti od fenološke faze
VL  - 37
IS  - 2
SP  - 97
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1879
ER  - 

@article{
author = "Slavkovska, Violeta and Zlatković, Bojan and Bräuchler, Christian and Stojanović, Danilo and Tzakou, Olga and Couladis, Maria",
year = "2013",
abstract = "The variability of essential oil characteristics in different phenological stages of Clinopodium pulegium from its natural habitat (Svrljiški Timok gorge, Serbia) and from cultivated plants (Niš, Serbia) was determined. The essential oils were obtained from aerial parts of the plants by hydrodistillation and analyzed by GC-MS. These are the first data on the essential oil characteristics of plants of the population from the Svrljiški Timok gorge. Samples originating from both natural and cultivated populations were characterized by high amounts of essential oils in all stages of development (0.8% - 1.4%). Twenty-one compounds were identified, representing 95.3-99.6% of the total oils. Differences in the essential oil composition were more quantitative than qualitative. The quality of the essential oil was stable and did not vary with differences in environmental conditions. Dominance of the main components was modified by phenological stage. Pulegone was dominant in the vegetative (76.1% wild population, 62.7% cultivated population) and the flowering (49.5%, 64.6%) stages, while menthone (48.5%, 65.3%) displaced pulegone (34.7%, 18.4%) at the fruiting stage. Cultivated plants in the vegetative and flowering stages can be considered a significant source of pulegone, and in the fruiting stage a significant source of menthone. Careful selection of the developmental stage of the plant is a potential tool which could be employed to obtain the preferred chemical composition of C. pulegium for commercial use., U radu je praćena promenljivost količine i sastava etarkog ulja Clinopodium pulegium kroz različite stadijume razvoja biljke (vegetativni, stadijum cvetanja i plodonošenja). Materijal je sakupljan iz samonikle (klisura Svrljiškog Timoka) i gajene (Niš) populacije. Etarsko ulje je izolovano iz nadzemnih delova biljaka destilacijom vodenom parom i analizirano GC-MS metodom. Ovo su prvi podaci o osobinama etarskog ulja biljaka populacije iz klisure Svrljiškog Timoka. Svi uzorci kako iz samonikle tako i iz gajene populacije su se odlikovali velikim sadržajem ulja (0.8% - 1.4%) u svim stadijumima razvoja. Dvadeset jedna komponenta je identifikovana što predstavlja 95.3-99.6% ukupnog etarskog ulja. Razlike u sastavu ulja su bile više kvantitativne nego kvalitativne. Kvalitet etarskog ulja je bio stabilan a variranja uslovljena promenom sredine relativno mala. Dominacija glavnih komponenata je bila uslovljena promenama fenoloških faza. Pulegon je bio dominantan u vegetativnoj (76.1% u ulju biljaka samonikle populacije, 62.7% u ulju biljaka gajene populacije) i fazi cvetanja (49.5%, 64.6%), dok je menton bio dominantan (48.5%, 65.3%) u fazi plodonošenja. Rezultati su pokazali da se biljke u vegetativnoj i fazi cvetanja mogu smatrati značajnim izvorom pulegona, a u fazi plodonošenja značajnim izvorom mentona. Pažljivim odabirom razvojnog stadijuma biljke moguće je dobiti etarsko ulje željenog sastava koje bi se moglo upotrebiti u komercijalne svrhe.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage, Promenljivost osobina etarskog ulja vrste Clinopodium pulegium (Lamiaceae) u zavisnosti od fenološke faze",
volume = "37",
number = "2",
pages = "97-104",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1879"
}

Slavkovska, V., Zlatković, B., Bräuchler, C., Stojanović, D., Tzakou, O.,& Couladis, M.. (2013). Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 37(2), 97-104.
https://hdl.handle.net/21.15107/rcub_farfar_1879

Slavkovska V, Zlatković B, Bräuchler C, Stojanović D, Tzakou O, Couladis M. Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage. in Botanica Serbica. 2013;37(2):97-104.
https://hdl.handle.net/21.15107/rcub_farfar_1879 .

Slavkovska, Violeta, Zlatković, Bojan, Bräuchler, Christian, Stojanović, Danilo, Tzakou, Olga, Couladis, Maria, "Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage" in Botanica Serbica, 37, no. 2 (2013):97-104,
https://hdl.handle.net/21.15107/rcub_farfar_1879 .

TY  - JOUR
AU  - Radovanović, Ana M.
AU  - Cupara, Snežana
AU  - Popović, Suzana L. J.
AU  - Tomović, Marina T.
AU  - Slavkovska, Violeta
AU  - Janković, Slobodan
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1869
AB  - Potentilla reptans L. belongs to the least studied of the plants from Rosaceae family, Potentilla genus. There are no data on cytotoxicity of P. reptans extracts, though traditionaly it was used as anti-inflammatory and antiinfective. The aim of these studies was to investigate potential antitumor activity of aqueous extracts (rhizome and aerial parts) of P. reptans on 4T1 mouse breast cancer cell line._Aqueous extracts of rhizome and aerial parts of P. reptans were tested for cytotoxicity by the MTT colorimetric assay on 4T1 cancer cell line in concentration range 100-800 mu g/mL. Aqueous extracts of P. reptans rhizome and aerial parts show concentration dependent cytotoxic effect in the range of tested concentrations. IC50 value of P. reptans rhizome extract was 280.51 +/- 1.16 mu g/mL. IC50 value of P. reptans aerial parts extract was 310.79 +/- 1.22 mu g/mL. The significant difference in cytotoxicity among tested concentrations was observed. Aqueous extracts of P. mptans rhizome and aerial parts demonstrated weak cytotoxic activity on 4T1 mouse breast cancer cell line, which is in correlation with current cytotoxicity data for aqueous herbal extracts. Rhizome extract of P. reptans has slightly higher antitumor activity than aerial parts extract. The results represent the first report on cytotoxicity for this plant and further research on human cell lines is indicated.
PB  - Polskie Towarzystwo Farmaceutyczne, Warsaw
T2  - Acta Poloniae Pharmaceutica - Drug Research
T1  - Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts
VL  - 70
IS  - 5
SP  - 851
EP  - 854
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1869
ER  - 

@article{
author = "Radovanović, Ana M. and Cupara, Snežana and Popović, Suzana L. J. and Tomović, Marina T. and Slavkovska, Violeta and Janković, Slobodan",
year = "2013",
abstract = "Potentilla reptans L. belongs to the least studied of the plants from Rosaceae family, Potentilla genus. There are no data on cytotoxicity of P. reptans extracts, though traditionaly it was used as anti-inflammatory and antiinfective. The aim of these studies was to investigate potential antitumor activity of aqueous extracts (rhizome and aerial parts) of P. reptans on 4T1 mouse breast cancer cell line._Aqueous extracts of rhizome and aerial parts of P. reptans were tested for cytotoxicity by the MTT colorimetric assay on 4T1 cancer cell line in concentration range 100-800 mu g/mL. Aqueous extracts of P. reptans rhizome and aerial parts show concentration dependent cytotoxic effect in the range of tested concentrations. IC50 value of P. reptans rhizome extract was 280.51 +/- 1.16 mu g/mL. IC50 value of P. reptans aerial parts extract was 310.79 +/- 1.22 mu g/mL. The significant difference in cytotoxicity among tested concentrations was observed. Aqueous extracts of P. mptans rhizome and aerial parts demonstrated weak cytotoxic activity on 4T1 mouse breast cancer cell line, which is in correlation with current cytotoxicity data for aqueous herbal extracts. Rhizome extract of P. reptans has slightly higher antitumor activity than aerial parts extract. The results represent the first report on cytotoxicity for this plant and further research on human cell lines is indicated.",
publisher = "Polskie Towarzystwo Farmaceutyczne, Warsaw",
journal = "Acta Poloniae Pharmaceutica - Drug Research",
title = "Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts",
volume = "70",
number = "5",
pages = "851-854",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1869"
}

Radovanović, A. M., Cupara, S., Popović, S. L. J., Tomović, M. T., Slavkovska, V.,& Janković, S.. (2013). Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts. in Acta Poloniae Pharmaceutica - Drug Research
Polskie Towarzystwo Farmaceutyczne, Warsaw., 70(5), 851-854.
https://hdl.handle.net/21.15107/rcub_farfar_1869

Radovanović AM, Cupara S, Popović SLJ, Tomović MT, Slavkovska V, Janković S. Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts. in Acta Poloniae Pharmaceutica - Drug Research. 2013;70(5):851-854.
https://hdl.handle.net/21.15107/rcub_farfar_1869 .

Radovanović, Ana M., Cupara, Snežana, Popović, Suzana L. J., Tomović, Marina T., Slavkovska, Violeta, Janković, Slobodan, "Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts" in Acta Poloniae Pharmaceutica - Drug Research, 70, no. 5 (2013):851-854,
https://hdl.handle.net/21.15107/rcub_farfar_1869 .