Kostić, Milica

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  • Kostić, Milica (2)
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Author's Bibliography

Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf

Pavlović, Dragana; Vukelić-Nikolić, Marija; Marčetić, Mirjana; Kitić, Dušanka; Kostić, Milica; Milutinović, Milica; Miladinović, Bojana

(Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 2019)

TY  - CONF
AU  - Pavlović, Dragana
AU  - Vukelić-Nikolić, Marija
AU  - Marčetić, Mirjana
AU  - Kitić, Dušanka
AU  - Kostić, Milica
AU  - Milutinović, Milica
AU  - Miladinović, Bojana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5305
AB  - Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf
SP  - 178
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5305
ER  - 
@conference{
author = "Pavlović, Dragana and Vukelić-Nikolić, Marija and Marčetić, Mirjana and Kitić, Dušanka and Kostić, Milica and Milutinović, Milica and Miladinović, Bojana",
year = "2019",
abstract = "Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf",
pages = "178-178",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5305"
}
Pavlović, D., Vukelić-Nikolić, M., Marčetić, M., Kitić, D., Kostić, M., Milutinović, M.,& Miladinović, B.. (2019). Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305
Pavlović D, Vukelić-Nikolić M, Marčetić M, Kitić D, Kostić M, Milutinović M, Miladinović B. Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305 .
Pavlović, Dragana, Vukelić-Nikolić, Marija, Marčetić, Mirjana, Kitić, Dušanka, Kostić, Milica, Milutinović, Milica, Miladinović, Bojana, "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):178-178,
https://hdl.handle.net/21.15107/rcub_farfar_5305 .

Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)

Pavlović, Dragana R.; Lakušić, Branislava; Kitić, Dušanka; Milutinović, Milica; Kostić, Milica; Miladinović, Bojana; Kovačević, Nada

(Univerzitet u Nišu - Medicinski fakultet, Niš, 2014)

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Lakušić, Branislava
AU  - Kitić, Dušanka
AU  - Milutinović, Milica
AU  - Kostić, Milica
AU  - Miladinović, Bojana
AU  - Kovačević, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2239
AB  - Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium).
AB  - Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)
T1  - Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)
VL  - 31
IS  - 1
SP  - 81
EP  - 85
DO  - 10.2478/afmnai-2014-0009
ER  - 
@article{
author = "Pavlović, Dragana R. and Lakušić, Branislava and Kitić, Dušanka and Milutinović, Milica and Kostić, Milica and Miladinović, Bojana and Kovačević, Nada",
year = "2014",
abstract = "Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium)., Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae), Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)",
volume = "31",
number = "1",
pages = "81-85",
doi = "10.2478/afmnai-2014-0009"
}
Pavlović, D. R., Lakušić, B., Kitić, D., Milutinović, M., Kostić, M., Miladinović, B.,& Kovačević, N.. (2014). Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 31(1), 81-85.
https://doi.org/10.2478/afmnai-2014-0009
Pavlović DR, Lakušić B, Kitić D, Milutinović M, Kostić M, Miladinović B, Kovačević N. Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis. 2014;31(1):81-85.
doi:10.2478/afmnai-2014-0009 .
Pavlović, Dragana R., Lakušić, Branislava, Kitić, Dušanka, Milutinović, Milica, Kostić, Milica, Miladinović, Bojana, Kovačević, Nada, "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)" in Acta Facultatis Medicae Naissensis, 31, no. 1 (2014):81-85,
https://doi.org/10.2478/afmnai-2014-0009 . .
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