TY  - CONF
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Rajab Elferjane, Rajab Elferjane
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5261
AB  - Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.
PB  - University of East Sarajevo, Faculty of technology
C3  - VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
T1  - Maceration and heat-assisted extraction of polyphenols from Aloe vera
SP  - 136
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5261
ER  - 

@conference{
author = "Čutović, Natalija and Jovanović, Aleksandra and Rajab Elferjane, Rajab Elferjane and Milutinović, Violeta and Petrović, Predrag and Marinković, Aleksandar and Bugarski, Branko",
year = "2023",
abstract = "Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.",
publisher = "University of East Sarajevo, Faculty of technology",
journal = "VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings",
title = "Maceration and heat-assisted extraction of polyphenols from Aloe vera",
pages = "136-143",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5261"
}

Čutović, N., Jovanović, A., Rajab Elferjane, R. E., Milutinović, V., Petrović, P., Marinković, A.,& Bugarski, B.. (2023). Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
University of East Sarajevo, Faculty of technology., 136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261

Čutović N, Jovanović A, Rajab Elferjane RE, Milutinović V, Petrović P, Marinković A, Bugarski B. Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings. 2023;:136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

Čutović, Natalija, Jovanović, Aleksandra, Rajab Elferjane, Rajab Elferjane, Milutinović, Violeta, Petrović, Predrag, Marinković, Aleksandar, Bugarski, Branko, "Maceration and heat-assisted extraction of polyphenols from Aloe vera" in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings (2023):136-143,
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, J.
AU  - Klaus, A.
AU  - Carević, Momir
AU  - Petković, Miloš
AU  - Vuković, N.
AU  - Cvetković, A.
AU  - Žižak, Željko
AU  - Bugarski, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3357
AB  - Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.
PB  - Elsevier Science BV, Amsterdam
T2  - Southern Medical Journal
T1  - From mycelium to spores: A whole circle of biological potency of mosaic puffball
VL  - 123
SP  - 152
EP  - 160
DO  - 10.1016/j.sajb.2019.03.016
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, J. and Klaus, A. and Carević, Momir and Petković, Miloš and Vuković, N. and Cvetković, A. and Žižak, Željko and Bugarski, Branko",
year = "2019",
abstract = "Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Southern Medical Journal",
title = "From mycelium to spores: A whole circle of biological potency of mosaic puffball",
volume = "123",
pages = "152-160",
doi = "10.1016/j.sajb.2019.03.016"
}

Petrović, P., Vunduk, J., Klaus, A., Carević, M., Petković, M., Vuković, N., Cvetković, A., Žižak, Ž.,& Bugarski, B.. (2019). From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal
Elsevier Science BV, Amsterdam., 123, 152-160.
https://doi.org/10.1016/j.sajb.2019.03.016

Petrović P, Vunduk J, Klaus A, Carević M, Petković M, Vuković N, Cvetković A, Žižak Ž, Bugarski B. From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal. 2019;123:152-160.
doi:10.1016/j.sajb.2019.03.016 .

Petrović, Predrag, Vunduk, J., Klaus, A., Carević, Momir, Petković, Miloš, Vuković, N., Cvetković, A., Žižak, Željko, Bugarski, Branko, "From mycelium to spores: A whole circle of biological potency of mosaic puffball" in Southern Medical Journal, 123 (2019):152-160,
https://doi.org/10.1016/j.sajb.2019.03.016 . .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Pantić, Milena
AU  - Belović, Miona
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Milić, Jela
AU  - Žilić, Slađana
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3427
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Pantić, Milena and Belović, Miona and Pavlović, Vladimir and Bugarski, Branko and Milić, Jela and Žilić, Slađana and Nedović, Viktor",
year = "2017",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}

Kalusević, A., Lević, S., Čalija, B., Pantić, M., Belović, M., Pavlović, V., Bugarski, B., Milić, J., Žilić, S.,& Nedović, V.. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939

Kalusević A, Lević S, Čalija B, Pantić M, Belović M, Pavlović V, Bugarski B, Milić J, Žilić S, Nedović V. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation. 2017;34(5):475-487.
doi:10.1080/02652048.2017.1354939 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Pantić, Milena, Belović, Miona, Pavlović, Vladimir, Bugarski, Branko, Milić, Jela, Žilić, Slađana, Nedović, Viktor, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" in Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 . .

TY  - JOUR
AU  - Mirković, Dušica
AU  - Ibrić, Svetlana
AU  - Balanc, Bojana
AU  - Knez, Željko
AU  - Bugarski, Branko
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2996
AB  - The aim of this study was to develop, characterize and evaluate concentrated nanoemulsions (20%) for parenteral nutrition. Those systems were developed by the high-pressure homogenization method. Optimal conditions for the nanoemulsion production were identified using 2(4-1) fractional factorial design. The characterization of physicochemical parameters was carried out immediately after the nanoemulsion production, and after 10 and 30 days. The biological control was conducted 30 days after their preparation as well. The oil phase contained the combination of the soybean (SO) and fish oil (FO) as well as the fish oil and medium-chain triglycerides (MCT), while the aqueous phase was composed of water for injections. The egg yolk phospholipids (EP) were used as surfactants alone, or in combination with Poloxamer 188 (PI). The obtained results were in accordance with the literature date e.i. quality requirements for parenteral emulsions (the droplet diameter  lt = 500 nm, PDI  lt = 0.25, absolute value of zeta-potential >= 25 mV, pH-value in the range of 6-9). It was shown that the combination of two surfactants (the egg yolk phospholipids that provides the electrostatic stabilization and Poloxamer 188-steric stabilizer) used as emulsifiers ensures the optimal quality of the obtained nanoemulsions for parenteral nutrition.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Drug Delivery Science and Technology
T1  - Evaluation of the impact of critical quality attributes and critical process parameters on quality and stability of parenteral nutrition nanoemulsions
VL  - 39
SP  - 341
EP  - 347
DO  - 10.1016/j.jddst.2017.04.004
ER  - 

@article{
author = "Mirković, Dušica and Ibrić, Svetlana and Balanc, Bojana and Knez, Željko and Bugarski, Branko",
year = "2017",
abstract = "The aim of this study was to develop, characterize and evaluate concentrated nanoemulsions (20%) for parenteral nutrition. Those systems were developed by the high-pressure homogenization method. Optimal conditions for the nanoemulsion production were identified using 2(4-1) fractional factorial design. The characterization of physicochemical parameters was carried out immediately after the nanoemulsion production, and after 10 and 30 days. The biological control was conducted 30 days after their preparation as well. The oil phase contained the combination of the soybean (SO) and fish oil (FO) as well as the fish oil and medium-chain triglycerides (MCT), while the aqueous phase was composed of water for injections. The egg yolk phospholipids (EP) were used as surfactants alone, or in combination with Poloxamer 188 (PI). The obtained results were in accordance with the literature date e.i. quality requirements for parenteral emulsions (the droplet diameter  lt = 500 nm, PDI  lt = 0.25, absolute value of zeta-potential >= 25 mV, pH-value in the range of 6-9). It was shown that the combination of two surfactants (the egg yolk phospholipids that provides the electrostatic stabilization and Poloxamer 188-steric stabilizer) used as emulsifiers ensures the optimal quality of the obtained nanoemulsions for parenteral nutrition.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Drug Delivery Science and Technology",
title = "Evaluation of the impact of critical quality attributes and critical process parameters on quality and stability of parenteral nutrition nanoemulsions",
volume = "39",
pages = "341-347",
doi = "10.1016/j.jddst.2017.04.004"
}

Mirković, D., Ibrić, S., Balanc, B., Knez, Ž.,& Bugarski, B.. (2017). Evaluation of the impact of critical quality attributes and critical process parameters on quality and stability of parenteral nutrition nanoemulsions. in Journal of Drug Delivery Science and Technology
Elsevier Science BV, Amsterdam., 39, 341-347.
https://doi.org/10.1016/j.jddst.2017.04.004

Mirković D, Ibrić S, Balanc B, Knez Ž, Bugarski B. Evaluation of the impact of critical quality attributes and critical process parameters on quality and stability of parenteral nutrition nanoemulsions. in Journal of Drug Delivery Science and Technology. 2017;39:341-347.
doi:10.1016/j.jddst.2017.04.004 .

Mirković, Dušica, Ibrić, Svetlana, Balanc, Bojana, Knez, Željko, Bugarski, Branko, "Evaluation of the impact of critical quality attributes and critical process parameters on quality and stability of parenteral nutrition nanoemulsions" in Journal of Drug Delivery Science and Technology, 39 (2017):341-347,
https://doi.org/10.1016/j.jddst.2017.04.004 . .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2876
AB  - The goal of this study was to investigate the characteristics of grape skin extract (GSE) spray dried with different carriers: maltodextrin (MD), gum Arabic (GA) and skim milk powder (SMP). The grape skin extract was obtained from winery by-product of red grape variety Prokupac (Vitis vinifera L.). The morphology of the powders, their thermal, chemical and physical properties (water activity, bulk and tapped densities, solubility), as well as release studies in different pH conditions were analyzed. Total anthocyanin content and total phenolic content were determined by spectrophotometric methods. MD and GA-based microparticles were non-porous and spherical, while SMP-based ones were irregularly shaped. The process of spray drying Prokupac GSE using these three carriers produced powders with low water activity (0.24-0.28), good powder characteristics, high yields, and solubility higher than 90%. The obtained dissolution/release profiles indicated prolonged release of anthocyanins and phenolic compounds in different mediums, especially from GSE/GA microparticles. These results have shown that grape skin as the main by-product of wine production could be used as a source of natural colorants and bioactive compounds, and microencapsulation as a promising technique for the protection of these compounds, their stabilization in longer periods and prolonged release.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract
VL  - 54
IS  - 11
SP  - 3411
EP  - 3420
DO  - 10.1007/s13197-017-2790-6
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Milić, Jela and Pavlović, Vladimir and Bugarski, Branko and Nedović, Viktor",
year = "2017",
abstract = "The goal of this study was to investigate the characteristics of grape skin extract (GSE) spray dried with different carriers: maltodextrin (MD), gum Arabic (GA) and skim milk powder (SMP). The grape skin extract was obtained from winery by-product of red grape variety Prokupac (Vitis vinifera L.). The morphology of the powders, their thermal, chemical and physical properties (water activity, bulk and tapped densities, solubility), as well as release studies in different pH conditions were analyzed. Total anthocyanin content and total phenolic content were determined by spectrophotometric methods. MD and GA-based microparticles were non-porous and spherical, while SMP-based ones were irregularly shaped. The process of spray drying Prokupac GSE using these three carriers produced powders with low water activity (0.24-0.28), good powder characteristics, high yields, and solubility higher than 90%. The obtained dissolution/release profiles indicated prolonged release of anthocyanins and phenolic compounds in different mediums, especially from GSE/GA microparticles. These results have shown that grape skin as the main by-product of wine production could be used as a source of natural colorants and bioactive compounds, and microencapsulation as a promising technique for the protection of these compounds, their stabilization in longer periods and prolonged release.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract",
volume = "54",
number = "11",
pages = "3411-3420",
doi = "10.1007/s13197-017-2790-6"
}

Kalusević, A., Lević, S., Čalija, B., Milić, J., Pavlović, V., Bugarski, B.,& Nedović, V.. (2017). Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 54(11), 3411-3420.
https://doi.org/10.1007/s13197-017-2790-6

Kalusević A, Lević S, Čalija B, Milić J, Pavlović V, Bugarski B, Nedović V. Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract. in Journal of Food Science and Technology-Mysore. 2017;54(11):3411-3420.
doi:10.1007/s13197-017-2790-6 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Milić, Jela, Pavlović, Vladimir, Bugarski, Branko, Nedović, Viktor, "Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract" in Journal of Food Science and Technology-Mysore, 54, no. 11 (2017):3411-3420,
https://doi.org/10.1007/s13197-017-2790-6 . .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Pantić, Milena
AU  - Belović, Miona
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Milić, Jela
AU  - Žilić, Slađana
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2797
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Pantić, Milena and Belović, Miona and Pavlović, Vladimir and Bugarski, Branko and Milić, Jela and Žilić, Slađana and Nedović, Viktor",
year = "2017",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}

Kalusević, A., Lević, S., Čalija, B., Pantić, M., Belović, M., Pavlović, V., Bugarski, B., Milić, J., Žilić, S.,& Nedović, V.. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939

Kalusević A, Lević S, Čalija B, Pantić M, Belović M, Pavlović V, Bugarski B, Milić J, Žilić S, Nedović V. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation. 2017;34(5):475-487.
doi:10.1080/02652048.2017.1354939 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Pantić, Milena, Belović, Miona, Pavlović, Vladimir, Bugarski, Branko, Milić, Jela, Žilić, Slađana, Nedović, Viktor, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" in Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 . .

TY  - JOUR
AU  - Cujić, Nada
AU  - Trifković, Kata
AU  - Bugarski, Branko
AU  - Ibrić, Svetlana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2544
AB  - Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system
VL  - 86
SP  - 120
EP  - 131
DO  - 10.1016/j.indcrop.2016.03.045
ER  - 

@article{
author = "Cujić, Nada and Trifković, Kata and Bugarski, Branko and Ibrić, Svetlana and Pljevljakusić, Dejan and Šavikin, Katarina",
year = "2016",
abstract = "Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system",
volume = "86",
pages = "120-131",
doi = "10.1016/j.indcrop.2016.03.045"
}

Cujić, N., Trifković, K., Bugarski, B., Ibrić, S., Pljevljakusić, D.,& Šavikin, K.. (2016). Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 86, 120-131.
https://doi.org/10.1016/j.indcrop.2016.03.045

Cujić N, Trifković K, Bugarski B, Ibrić S, Pljevljakusić D, Šavikin K. Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products. 2016;86:120-131.
doi:10.1016/j.indcrop.2016.03.045 .

Cujić, Nada, Trifković, Kata, Bugarski, Branko, Ibrić, Svetlana, Pljevljakusić, Dejan, Šavikin, Katarina, "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system" in Industrial Crops and Products, 86 (2016):120-131,
https://doi.org/10.1016/j.indcrop.2016.03.045 . .

TY  - JOUR
AU  - Sajc, L
AU  - Vunjak-Novaković, G
AU  - Grubišić, Dragoljub
AU  - Kovačević, Nada
AU  - Vuković, D
AU  - Bugarski, Branko
PY  - 1995
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/126
AB  - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
T2  - Applied Microbiology and Biotechnology
T1  - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
VL  - 43
IS  - 3
SP  - 416
EP  - 423
DO  - 10.1007/BF00218443
ER  - 

@article{
author = "Sajc, L and Vunjak-Novaković, G and Grubišić, Dragoljub and Kovačević, Nada and Vuković, D and Bugarski, Branko",
year = "1995",
abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.",
journal = "Applied Microbiology and Biotechnology",
title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor",
volume = "43",
number = "3",
pages = "416-423",
doi = "10.1007/BF00218443"
}

Sajc, L., Vunjak-Novaković, G., Grubišić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology, 43(3), 416-423.
https://doi.org/10.1007/BF00218443

Sajc L, Vunjak-Novaković G, Grubišić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423.
doi:10.1007/BF00218443 .

Sajc, L, Vunjak-Novaković, G, Grubišić, Dragoljub, Kovačević, Nada, Vuković, D, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423,
https://doi.org/10.1007/BF00218443 . .