TY  - JOUR
AU  - Grozdanić, Nađa
AU  - Đuričić, Ivana
AU  - Kosanić, Marijana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Etahiri, Samira
AU  - Assobhei, Omar
AU  - Benba, Jamila
AU  - Petović, Slavica
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3605
AB  - Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
PB  - Zerbinis Publications
T2  - Journal of B.U.ON.
T1  - Fucus spiralis extract and fractions: Anticancer and pharmacological potentials
VL  - 25
IS  - 2
SP  - 1219
EP  - 1229
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3605
ER  - 

@article{
author = "Grozdanić, Nađa and Đuričić, Ivana and Kosanić, Marijana and Zdunić, Gordana and Šavikin, Katarina and Etahiri, Samira and Assobhei, Omar and Benba, Jamila and Petović, Slavica and Matić, Ivana Z. and Stanojković, Tatjana P.",
year = "2020",
abstract = "Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.",
publisher = "Zerbinis Publications",
journal = "Journal of B.U.ON.",
title = "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials",
volume = "25",
number = "2",
pages = "1219-1229",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3605"
}

Grozdanić, N., Đuričić, I., Kosanić, M., Zdunić, G., Šavikin, K., Etahiri, S., Assobhei, O., Benba, J., Petović, S., Matić, I. Z.,& Stanojković, T. P.. (2020). Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.
Zerbinis Publications., 25(2), 1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605

Grozdanić N, Đuričić I, Kosanić M, Zdunić G, Šavikin K, Etahiri S, Assobhei O, Benba J, Petović S, Matić IZ, Stanojković TP. Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.. 2020;25(2):1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

Grozdanić, Nađa, Đuričić, Ivana, Kosanić, Marijana, Zdunić, Gordana, Šavikin, Katarina, Etahiri, Samira, Assobhei, Omar, Benba, Jamila, Petović, Slavica, Matić, Ivana Z., Stanojković, Tatjana P., "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials" in Journal of B.U.ON., 25, no. 2 (2020):1219-1229,
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

TY  - CONF
AU  - Pavlović, Dragana
AU  - Vukelić-Nikolić, Marija
AU  - Marčetić, Mirjana
AU  - Kitić, Dušanka
AU  - Kostić, Milica
AU  - Milutinović, Milica
AU  - Miladinović, Bojana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5305
AB  - Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf
SP  - 178
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5305
ER  - 

@conference{
author = "Pavlović, Dragana and Vukelić-Nikolić, Marija and Marčetić, Mirjana and Kitić, Dušanka and Kostić, Milica and Milutinović, Milica and Miladinović, Bojana",
year = "2019",
abstract = "Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf",
pages = "178-178",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5305"
}

Pavlović, D., Vukelić-Nikolić, M., Marčetić, M., Kitić, D., Kostić, M., Milutinović, M.,& Miladinović, B.. (2019). Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305

Pavlović D, Vukelić-Nikolić M, Marčetić M, Kitić D, Kostić M, Milutinović M, Miladinović B. Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305 .

Pavlović, Dragana, Vukelić-Nikolić, Marija, Marčetić, Mirjana, Kitić, Dušanka, Kostić, Milica, Milutinović, Milica, Miladinović, Bojana, "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):178-178,
https://hdl.handle.net/21.15107/rcub_farfar_5305 .

TY  - JOUR
AU  - Cujić, Nada
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Pljevljakusić, Dejan
AU  - Zdunić, Gordana
AU  - Ibrić, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2582
AB  - Traditional maceration method was used for the extraction of polyphenols from chokeberry (Aronia melanocarpa) dried fruit, and the effects of several extraction parameters on the total phenolics and anthocyanins contents were studied. Various solvents, particle size, solid solvent ratio and extraction time have been investigated as independent variables in two level factorial design. Among examined variables, time was not statistically important factor for the extraction of polyphenols. The optimal extraction conditions were maceration of 0.75 mm size berries by 50% ethanol, with solid solvent ratio of 1:20, and predicted values were 27.7 mg GAE/g for total phenolics and 0.27% for total anthocyanins. Under selected conditions, the experimental total phenolics were 27.8 mg GAE/g, and total anthocyanins were 0.27%, which is in agreement with the predicted values. In addition, a complementary quantitative analysis of individual phenolic compounds was performed using HPLC method. The study indicated that maceration was effective and simple technique for the extraction of bioactive compounds from chokeberry fruit.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique
VL  - 194
SP  - 135
EP  - 142
DO  - 10.1016/j.foodchem.2015.08.008
ER  - 

@article{
author = "Cujić, Nada and Šavikin, Katarina and Janković, Teodora and Pljevljakusić, Dejan and Zdunić, Gordana and Ibrić, Svetlana",
year = "2016",
abstract = "Traditional maceration method was used for the extraction of polyphenols from chokeberry (Aronia melanocarpa) dried fruit, and the effects of several extraction parameters on the total phenolics and anthocyanins contents were studied. Various solvents, particle size, solid solvent ratio and extraction time have been investigated as independent variables in two level factorial design. Among examined variables, time was not statistically important factor for the extraction of polyphenols. The optimal extraction conditions were maceration of 0.75 mm size berries by 50% ethanol, with solid solvent ratio of 1:20, and predicted values were 27.7 mg GAE/g for total phenolics and 0.27% for total anthocyanins. Under selected conditions, the experimental total phenolics were 27.8 mg GAE/g, and total anthocyanins were 0.27%, which is in agreement with the predicted values. In addition, a complementary quantitative analysis of individual phenolic compounds was performed using HPLC method. The study indicated that maceration was effective and simple technique for the extraction of bioactive compounds from chokeberry fruit.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique",
volume = "194",
pages = "135-142",
doi = "10.1016/j.foodchem.2015.08.008"
}

Cujić, N., Šavikin, K., Janković, T., Pljevljakusić, D., Zdunić, G.,& Ibrić, S.. (2016). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. in Food Chemistry
Elsevier Sci Ltd, Oxford., 194, 135-142.
https://doi.org/10.1016/j.foodchem.2015.08.008

Cujić N, Šavikin K, Janković T, Pljevljakusić D, Zdunić G, Ibrić S. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. in Food Chemistry. 2016;194:135-142.
doi:10.1016/j.foodchem.2015.08.008 .

Cujić, Nada, Šavikin, Katarina, Janković, Teodora, Pljevljakusić, Dejan, Zdunić, Gordana, Ibrić, Svetlana, "Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique" in Food Chemistry, 194 (2016):135-142,
https://doi.org/10.1016/j.foodchem.2015.08.008 . .

TY  - JOUR
AU  - Cujić, Nada
AU  - Trifković, Kata
AU  - Bugarski, Branko
AU  - Ibrić, Svetlana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2544
AB  - Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system
VL  - 86
SP  - 120
EP  - 131
DO  - 10.1016/j.indcrop.2016.03.045
ER  - 

@article{
author = "Cujić, Nada and Trifković, Kata and Bugarski, Branko and Ibrić, Svetlana and Pljevljakusić, Dejan and Šavikin, Katarina",
year = "2016",
abstract = "Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system",
volume = "86",
pages = "120-131",
doi = "10.1016/j.indcrop.2016.03.045"
}

Cujić, N., Trifković, K., Bugarski, B., Ibrić, S., Pljevljakusić, D.,& Šavikin, K.. (2016). Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 86, 120-131.
https://doi.org/10.1016/j.indcrop.2016.03.045

Cujić N, Trifković K, Bugarski B, Ibrić S, Pljevljakusić D, Šavikin K. Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products. 2016;86:120-131.
doi:10.1016/j.indcrop.2016.03.045 .

Cujić, Nada, Trifković, Kata, Bugarski, Branko, Ibrić, Svetlana, Pljevljakusić, Dejan, Šavikin, Katarina, "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system" in Industrial Crops and Products, 86 (2016):120-131,
https://doi.org/10.1016/j.indcrop.2016.03.045 . .

TY  - JOUR
AU  - Beara, Ivana
AU  - Živković, Jelena
AU  - Lesjak, Marija
AU  - Ristić, Jelena
AU  - Šavikin, Katarina
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2377
AB  - Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profile and anti-inflammatory activity of three Veronica species
VL  - 63
SP  - 276
EP  - 280
DO  - 10.1016/j.indcrop.2014.09.034
ER  - 

@article{
author = "Beara, Ivana and Živković, Jelena and Lesjak, Marija and Ristić, Jelena and Šavikin, Katarina and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profile and anti-inflammatory activity of three Veronica species",
volume = "63",
pages = "276-280",
doi = "10.1016/j.indcrop.2014.09.034"
}

Beara, I., Živković, J., Lesjak, M., Ristić, J., Šavikin, K., Maksimović, Z.,& Janković, T.. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 63, 276-280.
https://doi.org/10.1016/j.indcrop.2014.09.034

Beara I, Živković J, Lesjak M, Ristić J, Šavikin K, Maksimović Z, Janković T. Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products. 2015;63:276-280.
doi:10.1016/j.indcrop.2014.09.034 .

Beara, Ivana, Živković, Jelena, Lesjak, Marija, Ristić, Jelena, Šavikin, Katarina, Maksimović, Zoran, Janković, Teodora, "Phenolic profile and anti-inflammatory activity of three Veronica species" in Industrial Crops and Products, 63 (2015):276-280,
https://doi.org/10.1016/j.indcrop.2014.09.034 . .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Lakušić, Branislava
AU  - Kitić, Dušanka
AU  - Milutinović, Milica
AU  - Kostić, Milica
AU  - Miladinović, Bojana
AU  - Kovačević, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2239
AB  - Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium).
AB  - Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)
T1  - Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)
VL  - 31
IS  - 1
SP  - 81
EP  - 85
DO  - 10.2478/afmnai-2014-0009
ER  - 

@article{
author = "Pavlović, Dragana R. and Lakušić, Branislava and Kitić, Dušanka and Milutinović, Milica and Kostić, Milica and Miladinović, Bojana and Kovačević, Nada",
year = "2014",
abstract = "Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium)., Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae), Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)",
volume = "31",
number = "1",
pages = "81-85",
doi = "10.2478/afmnai-2014-0009"
}

Pavlović, D. R., Lakušić, B., Kitić, D., Milutinović, M., Kostić, M., Miladinović, B.,& Kovačević, N.. (2014). Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 31(1), 81-85.
https://doi.org/10.2478/afmnai-2014-0009

Pavlović DR, Lakušić B, Kitić D, Milutinović M, Kostić M, Miladinović B, Kovačević N. Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis. 2014;31(1):81-85.
doi:10.2478/afmnai-2014-0009 .

Pavlović, Dragana R., Lakušić, Branislava, Kitić, Dušanka, Milutinović, Milica, Kostić, Milica, Miladinović, Bojana, Kovačević, Nada, "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)" in Acta Facultatis Medicae Naissensis, 31, no. 1 (2014):81-85,
https://doi.org/10.2478/afmnai-2014-0009 . .

TY  - JOUR
AU  - Kardum, Nevena
AU  - Konić-Ristić, Aleksandra
AU  - Šavikin, Katarina
AU  - Spasić, Slavica
AU  - Stefanović, Aleksandra
AU  - Ivanišević, Jasmina
AU  - Miljković, Milica
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2100
AB  - Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P  lt  .001) and pro-oxidant-antioxidant balance (PAB; P  lt  .05), as well as increase in paroxonase-1 activity toward diazoxon (P  lt  .01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro-oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects
VL  - 17
IS  - 8
SP  - 869
EP  - 874
DO  - 10.1089/jmf.2013.0135
ER  - 

@article{
author = "Kardum, Nevena and Konić-Ristić, Aleksandra and Šavikin, Katarina and Spasić, Slavica and Stefanović, Aleksandra and Ivanišević, Jasmina and Miljković, Milica",
year = "2014",
abstract = "Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P  lt  .001) and pro-oxidant-antioxidant balance (PAB; P  lt  .05), as well as increase in paroxonase-1 activity toward diazoxon (P  lt  .01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro-oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects",
volume = "17",
number = "8",
pages = "869-874",
doi = "10.1089/jmf.2013.0135"
}

Kardum, N., Konić-Ristić, A., Šavikin, K., Spasić, S., Stefanović, A., Ivanišević, J.,& Miljković, M.. (2014). Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 17(8), 869-874.
https://doi.org/10.1089/jmf.2013.0135

Kardum N, Konić-Ristić A, Šavikin K, Spasić S, Stefanović A, Ivanišević J, Miljković M. Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects. in Journal of Medicinal Food. 2014;17(8):869-874.
doi:10.1089/jmf.2013.0135 .

Kardum, Nevena, Konić-Ristić, Aleksandra, Šavikin, Katarina, Spasić, Slavica, Stefanović, Aleksandra, Ivanišević, Jasmina, Miljković, Milica, "Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects" in Journal of Medicinal Food, 17, no. 8 (2014):869-874,
https://doi.org/10.1089/jmf.2013.0135 . .

TY  - JOUR
AU  - Konić-Ristić, Aleksandra
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Juranić, Zorica
AU  - Menković, Nebojša
AU  - Stanković, Ivan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1497
AB  - Total phenolics total anthocyanins mineral content radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries Total phenolic content ranged from 133 0 to 260 3 mg of gallic acid equivalents/100 g of fresh weight for red currant and black currant respectively Bilberry Juice contained the highest amount of total anthocyanins (0 18%) Significant correlation between total phenolics content and radical scavenging activity was observed (r = -0980 p  lt  0 01) All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10 2 to 70 5 mu l/ml Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa Fem X LS 174 MCF-7 and PC-3) Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells Fem X cells and MCF-7 cells were noticed Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Biological activity and chemical composition of different berry juices
VL  - 125
IS  - 4
SP  - 1412
EP  - 1417
DO  - 10.1016/j.foodchem.2010.10.018
ER  - 

@article{
author = "Konić-Ristić, Aleksandra and Šavikin, Katarina and Zdunić, Gordana and Janković, Teodora and Juranić, Zorica and Menković, Nebojša and Stanković, Ivan",
year = "2011",
abstract = "Total phenolics total anthocyanins mineral content radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries Total phenolic content ranged from 133 0 to 260 3 mg of gallic acid equivalents/100 g of fresh weight for red currant and black currant respectively Bilberry Juice contained the highest amount of total anthocyanins (0 18%) Significant correlation between total phenolics content and radical scavenging activity was observed (r = -0980 p  lt  0 01) All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10 2 to 70 5 mu l/ml Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa Fem X LS 174 MCF-7 and PC-3) Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells Fem X cells and MCF-7 cells were noticed Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Biological activity and chemical composition of different berry juices",
volume = "125",
number = "4",
pages = "1412-1417",
doi = "10.1016/j.foodchem.2010.10.018"
}

Konić-Ristić, A., Šavikin, K., Zdunić, G., Janković, T., Juranić, Z., Menković, N.,& Stanković, I.. (2011). Biological activity and chemical composition of different berry juices. in Food Chemistry
Elsevier Sci Ltd, Oxford., 125(4), 1412-1417.
https://doi.org/10.1016/j.foodchem.2010.10.018

Konić-Ristić A, Šavikin K, Zdunić G, Janković T, Juranić Z, Menković N, Stanković I. Biological activity and chemical composition of different berry juices. in Food Chemistry. 2011;125(4):1412-1417.
doi:10.1016/j.foodchem.2010.10.018 .

Konić-Ristić, Aleksandra, Šavikin, Katarina, Zdunić, Gordana, Janković, Teodora, Juranić, Zorica, Menković, Nebojša, Stanković, Ivan, "Biological activity and chemical composition of different berry juices" in Food Chemistry, 125, no. 4 (2011):1412-1417,
https://doi.org/10.1016/j.foodchem.2010.10.018 . .