TY  - JOUR
AU  - Srecković, Ivana
AU  - Birner-Gruenberger, Ruth
AU  - Besenboeck, Carolin
AU  - Miljković, Milica
AU  - Stojaković, Tatjana
AU  - Scharnagl, Hubert
AU  - Marsche, Gunther
AU  - Lang, Uwe
AU  - Kotur-Stevuljević, Jelena
AU  - Jelić-Ivanović, Zorana
AU  - Desoye, Gernot
AU  - Wadsack, Christian
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2129
AB  - Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p  lt  0.05) and SAA1 (increased, p  lt  0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated by lower activity (p  lt  0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p  lt  0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism.
PB  - Elsevier Science BV, Amsterdam
T2  - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
T1  - Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity
VL  - 1841
IS  - 11
SP  - 1619
EP  - 1627
DO  - 10.1016/j.bbalip.2014.07.021
ER  - 

@article{
author = "Srecković, Ivana and Birner-Gruenberger, Ruth and Besenboeck, Carolin and Miljković, Milica and Stojaković, Tatjana and Scharnagl, Hubert and Marsche, Gunther and Lang, Uwe and Kotur-Stevuljević, Jelena and Jelić-Ivanović, Zorana and Desoye, Gernot and Wadsack, Christian",
year = "2014",
abstract = "Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p  lt  0.05) and SAA1 (increased, p  lt  0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated by lower activity (p  lt  0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p  lt  0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
title = "Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity",
volume = "1841",
number = "11",
pages = "1619-1627",
doi = "10.1016/j.bbalip.2014.07.021"
}

Srecković, I., Birner-Gruenberger, R., Besenboeck, C., Miljković, M., Stojaković, T., Scharnagl, H., Marsche, G., Lang, U., Kotur-Stevuljević, J., Jelić-Ivanović, Z., Desoye, G.,& Wadsack, C.. (2014). Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity. in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Elsevier Science BV, Amsterdam., 1841(11), 1619-1627.
https://doi.org/10.1016/j.bbalip.2014.07.021

Srecković I, Birner-Gruenberger R, Besenboeck C, Miljković M, Stojaković T, Scharnagl H, Marsche G, Lang U, Kotur-Stevuljević J, Jelić-Ivanović Z, Desoye G, Wadsack C. Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity. in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2014;1841(11):1619-1627.
doi:10.1016/j.bbalip.2014.07.021 .

Srecković, Ivana, Birner-Gruenberger, Ruth, Besenboeck, Carolin, Miljković, Milica, Stojaković, Tatjana, Scharnagl, Hubert, Marsche, Gunther, Lang, Uwe, Kotur-Stevuljević, Jelena, Jelić-Ivanović, Zorana, Desoye, Gernot, Wadsack, Christian, "Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity" in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1841, no. 11 (2014):1619-1627,
https://doi.org/10.1016/j.bbalip.2014.07.021 . .