TY  - JOUR
AU  - Lunić, Tanja
AU  - Petković, Miloš
AU  - Rakić, Marija
AU  - Lađarević, Jelena
AU  - Repac, Jelena
AU  - Nedeljković, B.B.
AU  - Božić-Nedeljković, Biljana
PY  - 2025
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5750
AB  - This study explores the anti-neuroinflammatory and antioxidant potential of 42 hydroxybenzoic acid esters and three parent acids. Molecular docking simulations targeted key proteins involved in the lipopolysaccharide (LPS) signaling pathway to identify lead compounds for synthesis and in vitro evaluation. Among the screened esters, the most promising candidates demonstrated antioxidant activity comparable to vitamin C in ABTS and DPPH assays. Additionally, these esters significantly reduced the production of reactive oxygen species (ROS) and nitric oxide (NO) in H2O2- and LPS-stimulated BV2 microglial cells, indicating their ability to attenuate neuroinflammation. Further testing in SH-SY5Y neuronal cells exposed to microglia-derived supernatants confirmed the neuroprotective effects of these esters, reducing microglia-mediated neurotoxicity. These results suggest that hydroxybenzoic acid ester derivatives are promising candidates for mitigating microglia-driven neuroinflammation and protecting neurons, offering potential therapeutic applications for neurodegenerative diseases.
PB  - Elsevier
T2  - Journal of Molecular Structure
VL  - 1321
DO  - 10.1016/j.molstruc.2024.139804
ER  - 

@article{
author = "Lunić, Tanja and Petković, Miloš and Rakić, Marija and Lađarević, Jelena and Repac, Jelena and Nedeljković, B.B. and Božić-Nedeljković, Biljana",
year = "2025",
abstract = "This study explores the anti-neuroinflammatory and antioxidant potential of 42 hydroxybenzoic acid esters and three parent acids. Molecular docking simulations targeted key proteins involved in the lipopolysaccharide (LPS) signaling pathway to identify lead compounds for synthesis and in vitro evaluation. Among the screened esters, the most promising candidates demonstrated antioxidant activity comparable to vitamin C in ABTS and DPPH assays. Additionally, these esters significantly reduced the production of reactive oxygen species (ROS) and nitric oxide (NO) in H2O2- and LPS-stimulated BV2 microglial cells, indicating their ability to attenuate neuroinflammation. Further testing in SH-SY5Y neuronal cells exposed to microglia-derived supernatants confirmed the neuroprotective effects of these esters, reducing microglia-mediated neurotoxicity. These results suggest that hydroxybenzoic acid ester derivatives are promising candidates for mitigating microglia-driven neuroinflammation and protecting neurons, offering potential therapeutic applications for neurodegenerative diseases.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
volume = "1321",
doi = "10.1016/j.molstruc.2024.139804"
}

Lunić, T., Petković, M., Rakić, M., Lađarević, J., Repac, J., Nedeljković, B.B.,& Božić-Nedeljković, B.. (2025). in Journal of Molecular Structure
Elsevier., 1321.
https://doi.org/10.1016/j.molstruc.2024.139804

Lunić T, Petković M, Rakić M, Lađarević J, Repac J, Nedeljković B, Božić-Nedeljković B. in Journal of Molecular Structure. 2025;1321.
doi:10.1016/j.molstruc.2024.139804 .

Lunić, Tanja, Petković, Miloš, Rakić, Marija, Lađarević, Jelena, Repac, Jelena, Nedeljković, B.B., Božić-Nedeljković, Biljana, in Journal of Molecular Structure, 1321 (2025),
https://doi.org/10.1016/j.molstruc.2024.139804 . .

TY  - JOUR
AU  - Lukić, Milica
AU  - Ćirić, Ana
AU  - Božić, Dragana
AU  - Antić-Stanković, Jelena
AU  - Medarević, Đorđe
AU  - Maksimović, Zoran
PY  - 2024-09-07
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5749
AB  - Agricultural waste is underutilized, and sometimes burning them has a negative impact on the environment and human health. This research investigates the untapped potential of extracts from maize, wheat and sunflower waste as natural materials for cutaneous, specifically, cosmetic application. The possibility of incorporating lipid and ethanol extracts from wheat, maize, and sunflower into creams was investigated together with their potential contribution to the structural and functional properties of the topical formulations. Results of the physicochemical characterization show that investigated extracts can be successfully incorporated into creams with satisfactory stability. All extracts showed a desirable safety profile and good antimicrobial activity against various microorganisms. Lipid extracts have proven to be promising structural ingredients of the oil phase, contributing to the spreadability, occlusivity, and emollient effect. Ethanol extracts influenced washability and stickiness of the formulation and could be considered as prospective ingredients in self-preserving formulations. The extracts affected the sensory properties of the creams, mainly the smell and color. These results suggest that the extracts from wheat, maize, and sunflower waste could be used as multifunctional natural ingredients for cosmetic formulations which can replace less sustainable raw materials. This also represents a valorization of waste and is in line with broader sustainability goals.
PB  - MDPI
T2  - Pharmaceutics
T1  - Extracts from Wheat, Maize, and Sunflower Waste as Natural Raw Materials for Cosmetics: Value-Added Products Reaching Sustainability Goals
VL  - 16
IS  - 9
SP  - 1182
DO  - 10.3390/pharmaceutics16091182
ER  - 

@article{
author = "Lukić, Milica and Ćirić, Ana and Božić, Dragana and Antić-Stanković, Jelena and Medarević, Đorđe and Maksimović, Zoran",
year = "2024-09-07",
abstract = "Agricultural waste is underutilized, and sometimes burning them has a negative impact on the environment and human health. This research investigates the untapped potential of extracts from maize, wheat and sunflower waste as natural materials for cutaneous, specifically, cosmetic application. The possibility of incorporating lipid and ethanol extracts from wheat, maize, and sunflower into creams was investigated together with their potential contribution to the structural and functional properties of the topical formulations. Results of the physicochemical characterization show that investigated extracts can be successfully incorporated into creams with satisfactory stability. All extracts showed a desirable safety profile and good antimicrobial activity against various microorganisms. Lipid extracts have proven to be promising structural ingredients of the oil phase, contributing to the spreadability, occlusivity, and emollient effect. Ethanol extracts influenced washability and stickiness of the formulation and could be considered as prospective ingredients in self-preserving formulations. The extracts affected the sensory properties of the creams, mainly the smell and color. These results suggest that the extracts from wheat, maize, and sunflower waste could be used as multifunctional natural ingredients for cosmetic formulations which can replace less sustainable raw materials. This also represents a valorization of waste and is in line with broader sustainability goals.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Extracts from Wheat, Maize, and Sunflower Waste as Natural Raw Materials for Cosmetics: Value-Added Products Reaching Sustainability Goals",
volume = "16",
number = "9",
pages = "1182",
doi = "10.3390/pharmaceutics16091182"
}

Lukić, M., Ćirić, A., Božić, D., Antić-Stanković, J., Medarević, Đ.,& Maksimović, Z.. (2024-09-07). Extracts from Wheat, Maize, and Sunflower Waste as Natural Raw Materials for Cosmetics: Value-Added Products Reaching Sustainability Goals. in Pharmaceutics
MDPI., 16(9), 1182.
https://doi.org/10.3390/pharmaceutics16091182

Lukić M, Ćirić A, Božić D, Antić-Stanković J, Medarević Đ, Maksimović Z. Extracts from Wheat, Maize, and Sunflower Waste as Natural Raw Materials for Cosmetics: Value-Added Products Reaching Sustainability Goals. in Pharmaceutics. 2024;16(9):1182.
doi:10.3390/pharmaceutics16091182 .

Lukić, Milica, Ćirić, Ana, Božić, Dragana, Antić-Stanković, Jelena, Medarević, Đorđe, Maksimović, Zoran, "Extracts from Wheat, Maize, and Sunflower Waste as Natural Raw Materials for Cosmetics: Value-Added Products Reaching Sustainability Goals" in Pharmaceutics, 16, no. 9 (2024-09-07):1182,
https://doi.org/10.3390/pharmaceutics16091182 . .

TY  - JOUR
AU  - Lužaić, Tanja
AU  - Nedić Grujić, Katarina
AU  - Pezo, Lato
AU  - Nikolovski, Branislava
AU  - Maksimović, Zoran
AU  - Romanić, Ranko
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5764
AB  - In the production of refined sunflower oil, waxes are removed during the winterization stage, and wax crystals are separated through filtration assisted by filtration aids. Commonly used filtration aids in oil refining include perlite and diatomaceous earth. After winterization, a significant amount of filter cake remains as a by-product and is treated as waste. Today, natural cellulose fibers are being promoted as filtration aids. Their advantages are numerous, both in the production process and from an environmental perspective. However, their only disadvantage is their higher cost. Therefore, in this study, 57 filtration cycles during the industrial sunflower oil winterization step using cellulose-based filtration aids were monitored. Different process parameters, including the pressure differential on the filter, the flow rate of filtered oil, constant pressure period, the quantity of filtered oil, filtration time, the quantity of pre-coating and dosing filtration aids, the volume of filtered oil, the concentration of dosing filtration aid, as well as the mass of separated waxes, were observed. Additionally, artificial neural networks were applied to predict process parameters, optimize the process, and, above all, determine the dosage of filtration aids, which will make the process more economical. The optimal filtration process is performed at a pressure differential of 3.3 bar, lasting a total of 39 h, with 32 h at constant pressure, resulting in 322,503 kg of filtered oil and 90.41 kg of waxes. The optimal quantity of cellulose-based filtration aids employed for pre-coat was 80 kg, and for dosing, 375 kg, with an optimal concentration of 0.12% w/w.
PB  - MDPI
T2  - MDPI
T1  - Implementation of Cellulose-Based Filtration Aids in Industrial Sunflower Oil Dewaxing (Winterization): Process Monitoring, Prediction, and Optimization
VL  - 13
IS  - 18
SP  - 2960
DO  - 10.3390/foods13182960
ER  - 

@article{
author = "Lužaić, Tanja and Nedić Grujić, Katarina and Pezo, Lato and Nikolovski, Branislava and Maksimović, Zoran and Romanić, Ranko",
year = "2024",
abstract = "In the production of refined sunflower oil, waxes are removed during the winterization stage, and wax crystals are separated through filtration assisted by filtration aids. Commonly used filtration aids in oil refining include perlite and diatomaceous earth. After winterization, a significant amount of filter cake remains as a by-product and is treated as waste. Today, natural cellulose fibers are being promoted as filtration aids. Their advantages are numerous, both in the production process and from an environmental perspective. However, their only disadvantage is their higher cost. Therefore, in this study, 57 filtration cycles during the industrial sunflower oil winterization step using cellulose-based filtration aids were monitored. Different process parameters, including the pressure differential on the filter, the flow rate of filtered oil, constant pressure period, the quantity of filtered oil, filtration time, the quantity of pre-coating and dosing filtration aids, the volume of filtered oil, the concentration of dosing filtration aid, as well as the mass of separated waxes, were observed. Additionally, artificial neural networks were applied to predict process parameters, optimize the process, and, above all, determine the dosage of filtration aids, which will make the process more economical. The optimal filtration process is performed at a pressure differential of 3.3 bar, lasting a total of 39 h, with 32 h at constant pressure, resulting in 322,503 kg of filtered oil and 90.41 kg of waxes. The optimal quantity of cellulose-based filtration aids employed for pre-coat was 80 kg, and for dosing, 375 kg, with an optimal concentration of 0.12% w/w.",
publisher = "MDPI",
journal = "MDPI",
title = "Implementation of Cellulose-Based Filtration Aids in Industrial Sunflower Oil Dewaxing (Winterization): Process Monitoring, Prediction, and Optimization",
volume = "13",
number = "18",
pages = "2960",
doi = "10.3390/foods13182960"
}

Lužaić, T., Nedić Grujić, K., Pezo, L., Nikolovski, B., Maksimović, Z.,& Romanić, R.. (2024). Implementation of Cellulose-Based Filtration Aids in Industrial Sunflower Oil Dewaxing (Winterization): Process Monitoring, Prediction, and Optimization. in MDPI
MDPI., 13(18), 2960.
https://doi.org/10.3390/foods13182960

Lužaić T, Nedić Grujić K, Pezo L, Nikolovski B, Maksimović Z, Romanić R. Implementation of Cellulose-Based Filtration Aids in Industrial Sunflower Oil Dewaxing (Winterization): Process Monitoring, Prediction, and Optimization. in MDPI. 2024;13(18):2960.
doi:10.3390/foods13182960 .

Lužaić, Tanja, Nedić Grujić, Katarina, Pezo, Lato, Nikolovski, Branislava, Maksimović, Zoran, Romanić, Ranko, "Implementation of Cellulose-Based Filtration Aids in Industrial Sunflower Oil Dewaxing (Winterization): Process Monitoring, Prediction, and Optimization" in MDPI, 13, no. 18 (2024):2960,
https://doi.org/10.3390/foods13182960 . .

TY  - JOUR
AU  - Petković, Miloš
AU  - Kotur-Stevuljević, Jelena
AU  - Jovanović, Predrag
AU  - Jovanović, Miloš
AU  - Mitrović, Nikola
AU  - Simić, Milena
AU  - Tasić, Gordana
AU  - Savić, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5763
AB  - The  redox  balance  plays  a  crucial  role  in  maintaining  biological processes  under  normal  conditions.  Antioxidants  inhibit  and  reduce  harmful oxidation  processes,  while  pro-oxidants  can  act  as  anti-cancer  agents  by promoting  ROS-mediated  cell  death.  The  aim  of  this  study  is  to  compare  the redox  properties  of  seven  newly  synthesised  tribromopyrrole  derivatives  with three novel and four previously synthesized non-halogenated analogues in an in vitro  model  (in  human  serum)  and  with  exogenously  induced oxidative  stress. The  obtained  values  of  their  oxy  scores  (OS)  were  compared  and  the  result showed  that  four  non-halogenated  pyrrole  derivatives  with  secondary  amide group M2, M10,  M11  and M12  have  lower  OS  values  than  Trolox,  a  water-soluble  analogue  of  vitamin  E  with  proven  antioxidant  properties.  All  four compounds show strong resistance to oxidative stress, which is reflected in the maintenance of negative OS values when exposed to exogenous oxidative stress using TBH in the reaction mixture. This capability to resist invading ROS should be  expected  also  in  an  endogenous  environment,  where  constant  prooxidant production  takes  place  at  a  low,  homeostatic  level,  but  even  more  so  in pathological  conditions.  The  tribrominated  derivative M15 showed prooxidant activity  with  a  significantly  higher  OS  value  than  all  other  compounds  tested. The comparison of the dose-response of Trolox and the five compounds with the lowest OS also shows that compounds M2,  M7 and M10 have better antioxidant activity than Trolox.
AB  - Редоксравнотежа игра кључну улогу у одржавању биолошких процеса у нормалним условима.  Антиоксиданси  инхибирају  и  смањују  штетне  процесе  оксидације,  док прооксиданси  могу  деловати  као  антиканцерски  агенси  промовишући  ћелијску  смрт посредовану реактивним кисеоничним врстама. Циљ овог рада је да се упореде редокс својства седам  новосинтетисаних деривата трибромопирола и нехалогенованих аналога(три  новосинтетисанаи  четири  претходно  синтетисана) у in  vitroмоделу  (у  хуманом серуму)  и  са  егзогеноиндукованим  оксидативним  стресом.  Упоређене  су  добијене вредности њихових окси скорова (ОС) и резултат је показао да четири нехалогенована деривата  пирола  са  секундарном  амидном  групом М2,  М10,  М11и М12имају  ниже вредности  ОС  од  Тролокса,  аналога  витамина  Е  растворљивог  у  води  са  доказаним антиоксидативним  својствима.  Сва  четири  једињења  показују  јаку  отпорност  на оксидативни стрес, што се огледа у одржавању негативних вредности ОС када су изложене егзогеном оксидативном стресу коришћењем ТБХ у реакционој смеши. Ову способност да се одупируРОС треба очекивати и у ендогеном окружењу, где се константна производња прооксиданата  одвија  на  ниском,  хомеостатском  нивоу,  али  још  више  у  патолошким стањима.Трибромовани дериват М15је показао прооксидативну активност са значајно вишом ОС вредношћу од свих осталих тестираних једињења. Такође, упоредне анализе доза-одговор тестираних пет једињења са најнижим ОС и Тролокса показују да Тролокс има слабијуантиоксидативну активност од једињења М2,  М7и М10.
PB  - Serbian Chemical Society.
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and in vitro study of redox properties of pyrrole and halogenated pyrrole derivatives
T1  - Синтеза и in vitro студија редокс особина халогенованих и нехалогенованих деривата пирола
DO  - 10.2298/JSC240515082P
ER  - 

@article{
author = "Petković, Miloš and Kotur-Stevuljević, Jelena and Jovanović, Predrag and Jovanović, Miloš and Mitrović, Nikola and Simić, Milena and Tasić, Gordana and Savić, Vladimir",
year = "2024",
abstract = "The  redox  balance  plays  a  crucial  role  in  maintaining  biological processes  under  normal  conditions.  Antioxidants  inhibit  and  reduce  harmful oxidation  processes,  while  pro-oxidants  can  act  as  anti-cancer  agents  by promoting  ROS-mediated  cell  death.  The  aim  of  this  study  is  to  compare  the redox  properties  of  seven  newly  synthesised  tribromopyrrole  derivatives  with three novel and four previously synthesized non-halogenated analogues in an in vitro  model  (in  human  serum)  and  with  exogenously  induced oxidative  stress. The  obtained  values  of  their  oxy  scores  (OS)  were  compared  and  the  result showed  that  four  non-halogenated  pyrrole  derivatives  with  secondary  amide group M2, M10,  M11  and M12  have  lower  OS  values  than  Trolox,  a  water-soluble  analogue  of  vitamin  E  with  proven  antioxidant  properties.  All  four compounds show strong resistance to oxidative stress, which is reflected in the maintenance of negative OS values when exposed to exogenous oxidative stress using TBH in the reaction mixture. This capability to resist invading ROS should be  expected  also  in  an  endogenous  environment,  where  constant  prooxidant production  takes  place  at  a  low,  homeostatic  level,  but  even  more  so  in pathological  conditions.  The  tribrominated  derivative M15 showed prooxidant activity  with  a  significantly  higher  OS  value  than  all  other  compounds  tested. The comparison of the dose-response of Trolox and the five compounds with the lowest OS also shows that compounds M2,  M7 and M10 have better antioxidant activity than Trolox., Редоксравнотежа игра кључну улогу у одржавању биолошких процеса у нормалним условима.  Антиоксиданси  инхибирају  и  смањују  штетне  процесе  оксидације,  док прооксиданси  могу  деловати  као  антиканцерски  агенси  промовишући  ћелијску  смрт посредовану реактивним кисеоничним врстама. Циљ овог рада је да се упореде редокс својства седам  новосинтетисаних деривата трибромопирола и нехалогенованих аналога(три  новосинтетисанаи  четири  претходно  синтетисана) у in  vitroмоделу  (у  хуманом серуму)  и  са  егзогеноиндукованим  оксидативним  стресом.  Упоређене  су  добијене вредности њихових окси скорова (ОС) и резултат је показао да четири нехалогенована деривата  пирола  са  секундарном  амидном  групом М2,  М10,  М11и М12имају  ниже вредности  ОС  од  Тролокса,  аналога  витамина  Е  растворљивог  у  води  са  доказаним антиоксидативним  својствима.  Сва  четири  једињења  показују  јаку  отпорност  на оксидативни стрес, што се огледа у одржавању негативних вредности ОС када су изложене егзогеном оксидативном стресу коришћењем ТБХ у реакционој смеши. Ову способност да се одупируРОС треба очекивати и у ендогеном окружењу, где се константна производња прооксиданата  одвија  на  ниском,  хомеостатском  нивоу,  али  још  више  у  патолошким стањима.Трибромовани дериват М15је показао прооксидативну активност са значајно вишом ОС вредношћу од свих осталих тестираних једињења. Такође, упоредне анализе доза-одговор тестираних пет једињења са најнижим ОС и Тролокса показују да Тролокс има слабијуантиоксидативну активност од једињења М2,  М7и М10.",
publisher = "Serbian Chemical Society.",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and in vitro study of redox properties of pyrrole and halogenated pyrrole derivatives, Синтеза и in vitro студија редокс особина халогенованих и нехалогенованих деривата пирола",
doi = "10.2298/JSC240515082P"
}

Petković, M., Kotur-Stevuljević, J., Jovanović, P., Jovanović, M., Mitrović, N., Simić, M., Tasić, G.,& Savić, V.. (2024). Synthesis and in vitro study of redox properties of pyrrole and halogenated pyrrole derivatives. in Journal of the Serbian Chemical Society
Serbian Chemical Society...
https://doi.org/10.2298/JSC240515082P

Petković M, Kotur-Stevuljević J, Jovanović P, Jovanović M, Mitrović N, Simić M, Tasić G, Savić V. Synthesis and in vitro study of redox properties of pyrrole and halogenated pyrrole derivatives. in Journal of the Serbian Chemical Society. 2024;.
doi:10.2298/JSC240515082P .

Petković, Miloš, Kotur-Stevuljević, Jelena, Jovanović, Predrag, Jovanović, Miloš, Mitrović, Nikola, Simić, Milena, Tasić, Gordana, Savić, Vladimir, "Synthesis and in vitro study of redox properties of pyrrole and halogenated pyrrole derivatives" in Journal of the Serbian Chemical Society (2024),
https://doi.org/10.2298/JSC240515082P . .

TY  - JOUR
AU  - Živančević, Katarina
AU  - Aru, Başak
AU  - Demir, Abdullah
AU  - Radenović, Lidija
AU  - Anđus, Pavle
AU  - Yanıkkaya Demirel, Demirel
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5761
AB  - Background: Zinc (Zn), an essential micronutrient, regulates and maintains neurological functions. However, both Zn deficiency and excess can cause oxidative stress and neurodegenerative diseases. As previously reported, immunoglobulin G (IgG) can modulate oxidative stress in various disorders. Aims: To investigate whether IgG treatment can alleviate oxidative stress caused by Zn0 on microglia in vitro. Study Design: In vitro study. Methods: The feasibility of Zn0 treatment was evaluated using the MTS assay. Oxidative stress following treatment with Zn0, either alone or with IgG supplementation, was determined with dihydrorhodamine 123 staining. Flow cytometry was employed to ascertain the intracellular protein levels of TRIM21, PINK, PARKIN, MFN2, Beclin-1, and active LC3B. Methods: The feasibility of Zn0 treatment was evaluated using the MTS assay. Oxidative stress following treatment with Zn0, either alone or with IgG supplementation, was determined with dihydrorhodamine 123 staining. Flow cytometry was employed to ascertain the intracellular protein levels of TRIM21, PINK, PARKIN, MFN2, Beclin-1, and active LC3B. Results: In silico screening confirmed the association between Zn0 cytotoxicity and apoptosis. Furthermore, oxidative stress was identified as a critical mechanism that underlies Zn0 neurotoxicity. The in silico analysis revealed that Zn can interact with the constant region of the Ig heavy chain, suggesting a potential role for IgG in alleviating Zn0-induced cytotoxicity. Experimental findings supported this hypothesis, as IgG administration significantly reduced Zn0-induced mitochondrial stress in a dose-dependent manner. The upregulation of PINK1 levels by Zn0 exposure suggests that mitochondrial injury promotes mitophagy. Interestingly, Zn0 decreased TRIM21 levels, which is reversed by IgG administration. Conclusion: These findings elucidate the cellular responses to Zn0 and highlight the potential use of intravenous immunoglobulin in mitigating the adverse effects of acute Zn0 exposure.
PB  - Trakya University School of Medicine
T2  - Balkan medical journal
T1  - Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro
VL  - 41
IS  - 5
SP  - 348
EP  - 356
DO  - 10.4274/balkanmedj.galenos.2024.2024-4-119
ER  - 

@article{
author = "Živančević, Katarina and Aru, Başak and Demir, Abdullah and Radenović, Lidija and Anđus, Pavle and Yanıkkaya Demirel, Demirel",
year = "2024",
abstract = "Background: Zinc (Zn), an essential micronutrient, regulates and maintains neurological functions. However, both Zn deficiency and excess can cause oxidative stress and neurodegenerative diseases. As previously reported, immunoglobulin G (IgG) can modulate oxidative stress in various disorders. Aims: To investigate whether IgG treatment can alleviate oxidative stress caused by Zn0 on microglia in vitro. Study Design: In vitro study. Methods: The feasibility of Zn0 treatment was evaluated using the MTS assay. Oxidative stress following treatment with Zn0, either alone or with IgG supplementation, was determined with dihydrorhodamine 123 staining. Flow cytometry was employed to ascertain the intracellular protein levels of TRIM21, PINK, PARKIN, MFN2, Beclin-1, and active LC3B. Methods: The feasibility of Zn0 treatment was evaluated using the MTS assay. Oxidative stress following treatment with Zn0, either alone or with IgG supplementation, was determined with dihydrorhodamine 123 staining. Flow cytometry was employed to ascertain the intracellular protein levels of TRIM21, PINK, PARKIN, MFN2, Beclin-1, and active LC3B. Results: In silico screening confirmed the association between Zn0 cytotoxicity and apoptosis. Furthermore, oxidative stress was identified as a critical mechanism that underlies Zn0 neurotoxicity. The in silico analysis revealed that Zn can interact with the constant region of the Ig heavy chain, suggesting a potential role for IgG in alleviating Zn0-induced cytotoxicity. Experimental findings supported this hypothesis, as IgG administration significantly reduced Zn0-induced mitochondrial stress in a dose-dependent manner. The upregulation of PINK1 levels by Zn0 exposure suggests that mitochondrial injury promotes mitophagy. Interestingly, Zn0 decreased TRIM21 levels, which is reversed by IgG administration. Conclusion: These findings elucidate the cellular responses to Zn0 and highlight the potential use of intravenous immunoglobulin in mitigating the adverse effects of acute Zn0 exposure.",
publisher = "Trakya University School of Medicine",
journal = "Balkan medical journal",
title = "Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro",
volume = "41",
number = "5",
pages = "348-356",
doi = "10.4274/balkanmedj.galenos.2024.2024-4-119"
}

Živančević, K., Aru, B., Demir, A., Radenović, L., Anđus, P.,& Yanıkkaya Demirel, D.. (2024). Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro. in Balkan medical journal
Trakya University School of Medicine., 41(5), 348-356.
https://doi.org/10.4274/balkanmedj.galenos.2024.2024-4-119

Živančević K, Aru B, Demir A, Radenović L, Anđus P, Yanıkkaya Demirel D. Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro. in Balkan medical journal. 2024;41(5):348-356.
doi:10.4274/balkanmedj.galenos.2024.2024-4-119 .

Živančević, Katarina, Aru, Başak, Demir, Abdullah, Radenović, Lidija, Anđus, Pavle, Yanıkkaya Demirel, Demirel, "Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro" in Balkan medical journal, 41, no. 5 (2024):348-356,
https://doi.org/10.4274/balkanmedj.galenos.2024.2024-4-119 . .

TY  - JOUR
AU  - Jelić, Ratomir
AU  - Mrkalić, Emina
AU  - Ćendić-Serafinović, Marina
AU  - Odović, Jadranka
AU  - Sovrlić, Miroslav
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5760
AB  - The interaction between linamarin (LIN), a cyanogenic glycoside, and human serum albumin (HSA) was studied by multiple spectroscopic techniques and molecular docking simulation. All measurements were performed under physiological conditions. The obtained results (including the binding constants, effective quenching constant and a number of binding sites) showed that the complex of HSA-LIN is formed. The values of Stern-Volmer constants (6.70×103, 5.53×103 and 1.95×103) indicate that fluorescence quenching of HSA was static. Results of site marker experiments showed that the binding site of LIN is mainly located in site I (subdomain IIA) of HSA. The thermodynamic parameters showed that binding process occurs spontaneously through hydrophobic interactions. Molecular docking results are in good agreement with experimental data. Furthermore, computational results revealed LIN binds in the cavity of TRP 214, that is, subdomain IIA (site I) of HSA. This comprehensive study provides a deeper insight into ligand binding in HSA which may be useful in drug design and pharmacology.
PB  - John Wiley and Sons Inc
T2  - ChemistrySelect
VL  - 9
IS  - 35
DO  - 10.1002/slct.202401946
ER  - 

@article{
author = "Jelić, Ratomir and Mrkalić, Emina and Ćendić-Serafinović, Marina and Odović, Jadranka and Sovrlić, Miroslav",
year = "2024",
abstract = "The interaction between linamarin (LIN), a cyanogenic glycoside, and human serum albumin (HSA) was studied by multiple spectroscopic techniques and molecular docking simulation. All measurements were performed under physiological conditions. The obtained results (including the binding constants, effective quenching constant and a number of binding sites) showed that the complex of HSA-LIN is formed. The values of Stern-Volmer constants (6.70×103, 5.53×103 and 1.95×103) indicate that fluorescence quenching of HSA was static. Results of site marker experiments showed that the binding site of LIN is mainly located in site I (subdomain IIA) of HSA. The thermodynamic parameters showed that binding process occurs spontaneously through hydrophobic interactions. Molecular docking results are in good agreement with experimental data. Furthermore, computational results revealed LIN binds in the cavity of TRP 214, that is, subdomain IIA (site I) of HSA. This comprehensive study provides a deeper insight into ligand binding in HSA which may be useful in drug design and pharmacology.",
publisher = "John Wiley and Sons Inc",
journal = "ChemistrySelect",
volume = "9",
number = "35",
doi = "10.1002/slct.202401946"
}

Jelić, R., Mrkalić, E., Ćendić-Serafinović, M., Odović, J.,& Sovrlić, M.. (2024). in ChemistrySelect
John Wiley and Sons Inc., 9(35).
https://doi.org/10.1002/slct.202401946

Jelić R, Mrkalić E, Ćendić-Serafinović M, Odović J, Sovrlić M. in ChemistrySelect. 2024;9(35).
doi:10.1002/slct.202401946 .

Jelić, Ratomir, Mrkalić, Emina, Ćendić-Serafinović, Marina, Odović, Jadranka, Sovrlić, Miroslav, in ChemistrySelect, 9, no. 35 (2024),
https://doi.org/10.1002/slct.202401946 . .

TY  - JOUR
AU  - Kocić, Jovana
AU  - Zečević, Nebojša
AU  - Jagodić, Jovana
AU  - Ardalić, Daniela
AU  - Kotur-Stevuljević, Jelena
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5759
AB  - Background: Cervical intraepithelial neoplasia (CIN) represents a premalignant state presumably related to perturbations in circulating levels of trace elements. Materials and methods: Employing inductively coupled plasma mass spectrometry (ICP-MS), we quantified essential and toxic trace elements in the sera of 60 women diagnosed with CIN and 60 age-matched healthy counterparts. Results: Our investigation revealed a noteworthy higher levels in serum of Mn, Zn, and Pb, as well as lower levels in Ni, Se, Rb, and Mo levels within the CIN cohort. Levels of Mn, Zn, and Pb were higher by approximately 5.5-fold, 3.0-fold, and 7.5-fold, respectively, while Mo levels exhibited an approximate 4.5-fold reduction in CIN sera compared to the control group. While the study provided valuable insights into trace element variations, it's important to note that the adult Serbian population is considered Zn-deficient, so the Zn data should be interpreted with caution. Age stratification (30–40 vs. 40–50 vs. 50–60 years), smoking status (smokers vs. nonsmokers), and CIN severity (CIN 2 vs. CIN 3) yielded no significant disparities in elemental profiles. Among the 10 proposed ratios, 5 demonstrated a significant surge in CIN sera relative to controls: Mn/Se, Mn/Mo, Zn/Se, Zn/Mo, and Se/Mo. Correlation analysis of trace element levels revealed a predominantly consistent pattern between CIN cases and healthy subjects, except for Zn and its negative correlations (antagonistic interactions) with other analyzed trace elements. Conclusion: Our findings underscore differences in serum levels of specific trace elements in CIN cases versus controls, implicating their potential involvement in the underlying pathophysiological cascades culminating in cervical neoplasms.
PB  - Elsevier GmbH
T2  - Journal of Trace Elements in Medicine and Biology
VL  - 86
DO  - 10.1016/j.jtemb.2024.127531
ER  - 

@article{
author = "Kocić, Jovana and Zečević, Nebojša and Jagodić, Jovana and Ardalić, Daniela and Kotur-Stevuljević, Jelena and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2024",
abstract = "Background: Cervical intraepithelial neoplasia (CIN) represents a premalignant state presumably related to perturbations in circulating levels of trace elements. Materials and methods: Employing inductively coupled plasma mass spectrometry (ICP-MS), we quantified essential and toxic trace elements in the sera of 60 women diagnosed with CIN and 60 age-matched healthy counterparts. Results: Our investigation revealed a noteworthy higher levels in serum of Mn, Zn, and Pb, as well as lower levels in Ni, Se, Rb, and Mo levels within the CIN cohort. Levels of Mn, Zn, and Pb were higher by approximately 5.5-fold, 3.0-fold, and 7.5-fold, respectively, while Mo levels exhibited an approximate 4.5-fold reduction in CIN sera compared to the control group. While the study provided valuable insights into trace element variations, it's important to note that the adult Serbian population is considered Zn-deficient, so the Zn data should be interpreted with caution. Age stratification (30–40 vs. 40–50 vs. 50–60 years), smoking status (smokers vs. nonsmokers), and CIN severity (CIN 2 vs. CIN 3) yielded no significant disparities in elemental profiles. Among the 10 proposed ratios, 5 demonstrated a significant surge in CIN sera relative to controls: Mn/Se, Mn/Mo, Zn/Se, Zn/Mo, and Se/Mo. Correlation analysis of trace element levels revealed a predominantly consistent pattern between CIN cases and healthy subjects, except for Zn and its negative correlations (antagonistic interactions) with other analyzed trace elements. Conclusion: Our findings underscore differences in serum levels of specific trace elements in CIN cases versus controls, implicating their potential involvement in the underlying pathophysiological cascades culminating in cervical neoplasms.",
publisher = "Elsevier GmbH",
journal = "Journal of Trace Elements in Medicine and Biology",
volume = "86",
doi = "10.1016/j.jtemb.2024.127531"
}

Kocić, J., Zečević, N., Jagodić, J., Ardalić, D., Kotur-Stevuljević, J., Manojlović, D.,& Stojsavljević, A.. (2024). in Journal of Trace Elements in Medicine and Biology
Elsevier GmbH., 86.
https://doi.org/10.1016/j.jtemb.2024.127531

Kocić J, Zečević N, Jagodić J, Ardalić D, Kotur-Stevuljević J, Manojlović D, Stojsavljević A. in Journal of Trace Elements in Medicine and Biology. 2024;86.
doi:10.1016/j.jtemb.2024.127531 .

Kocić, Jovana, Zečević, Nebojša, Jagodić, Jovana, Ardalić, Daniela, Kotur-Stevuljević, Jelena, Manojlović, Dragan, Stojsavljević, Aleksandar, in Journal of Trace Elements in Medicine and Biology, 86 (2024),
https://doi.org/10.1016/j.jtemb.2024.127531 . .

TY  - JOUR
AU  - Marinko, Marija
AU  - Stojanović, Ivan
AU  - Milojević, Predrag
AU  - Nenezić, Dragoslav
AU  - Kanjuh, Vladimir
AU  - Yang, Qin
AU  - He, Guo-Wei
AU  - Novaković, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5762
AB  - Background: Changes in K+ channel expression/function are associated with disruption of vascular reactivity in several pathological conditions, including hypertension, diabetes, and atherosclerosis. Gasotransmitters achieve part of their effects in the organism by regulating ion channels, especially K+ channels. Their involvement in hydrogen sulfide (H2S)-mediated vasorelaxation is still unclear, and data about human vessels are limited. Objective: To determine the role of K+ channel subtypes in the vasorelaxant mechanism of H2S donor, sodium-hydrosulfide (NaHS), on isolated human internal mammary artery (HIMA). Results: NaHS (1 × 10−6–3 × 10−3 mol/L) induced a concentration-dependent relaxation of HIMA pre-contracted by phenylephrine and high K+. Among K+ channel blockers, iberiotoxin, glibenclamide, 4-aminopyridine (4-AP), and margatoxin significantly inhibited NaHS-induced relaxation of phenylephrine-contracted HIMA (P < 0.01), whereas in the presence of apamin/1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34) combination, the HIMA relaxation was partially reduced (P < 0.05). The effect of NaHS was antagonized by NO pathway inhibitors, L-NAME and KT5823, and by cyclo-oxygenase inhibitor, indomethacin (P < 0.01). Under conditions of blocked NO/prostacyclin synthesis and release, apamin/TRAM-34 and glibenclamide caused further decrease in NaHS-induced vasorelaxation (P < 0.01), while iberiotoxin, 4-AP, and margatoxin were without additional effect (P > 0.05). In the presence of nifedipine, NaHS induced partial relaxation of HIMA (P < 0.01). Conclusion: Our results demonstrated that H2S donor, NaHS, induced concentration-dependent relaxation of isolated HIMA. Vasorelaxant mechanisms of H2S included direct or indirect opening of different K+ channel subtypes, KATP, BKCa, SKCa/IKCa, and KV (subtype KV1.3), in addition to NO pathway activation and interference with extracellular Ca2+ influx.
PB  - John Wiley and Sons Inc
T2  - Fundamental and Clinical Pharmacology
T1  - Involvement of different K+ channel subtypes in hydrogen sulfide-induced vasorelaxation of human internal mammary artery
DO  - 10.1111/fcp.13036
ER  - 

@article{
author = "Marinko, Marija and Stojanović, Ivan and Milojević, Predrag and Nenezić, Dragoslav and Kanjuh, Vladimir and Yang, Qin and He, Guo-Wei and Novaković, Aleksandra",
year = "2024",
abstract = "Background: Changes in K+ channel expression/function are associated with disruption of vascular reactivity in several pathological conditions, including hypertension, diabetes, and atherosclerosis. Gasotransmitters achieve part of their effects in the organism by regulating ion channels, especially K+ channels. Their involvement in hydrogen sulfide (H2S)-mediated vasorelaxation is still unclear, and data about human vessels are limited. Objective: To determine the role of K+ channel subtypes in the vasorelaxant mechanism of H2S donor, sodium-hydrosulfide (NaHS), on isolated human internal mammary artery (HIMA). Results: NaHS (1 × 10−6–3 × 10−3 mol/L) induced a concentration-dependent relaxation of HIMA pre-contracted by phenylephrine and high K+. Among K+ channel blockers, iberiotoxin, glibenclamide, 4-aminopyridine (4-AP), and margatoxin significantly inhibited NaHS-induced relaxation of phenylephrine-contracted HIMA (P < 0.01), whereas in the presence of apamin/1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34) combination, the HIMA relaxation was partially reduced (P < 0.05). The effect of NaHS was antagonized by NO pathway inhibitors, L-NAME and KT5823, and by cyclo-oxygenase inhibitor, indomethacin (P < 0.01). Under conditions of blocked NO/prostacyclin synthesis and release, apamin/TRAM-34 and glibenclamide caused further decrease in NaHS-induced vasorelaxation (P < 0.01), while iberiotoxin, 4-AP, and margatoxin were without additional effect (P > 0.05). In the presence of nifedipine, NaHS induced partial relaxation of HIMA (P < 0.01). Conclusion: Our results demonstrated that H2S donor, NaHS, induced concentration-dependent relaxation of isolated HIMA. Vasorelaxant mechanisms of H2S included direct or indirect opening of different K+ channel subtypes, KATP, BKCa, SKCa/IKCa, and KV (subtype KV1.3), in addition to NO pathway activation and interference with extracellular Ca2+ influx.",
publisher = "John Wiley and Sons Inc",
journal = "Fundamental and Clinical Pharmacology",
title = "Involvement of different K+ channel subtypes in hydrogen sulfide-induced vasorelaxation of human internal mammary artery",
doi = "10.1111/fcp.13036"
}

Marinko, M., Stojanović, I., Milojević, P., Nenezić, D., Kanjuh, V., Yang, Q., He, G.,& Novaković, A.. (2024). Involvement of different K+ channel subtypes in hydrogen sulfide-induced vasorelaxation of human internal mammary artery. in Fundamental and Clinical Pharmacology
John Wiley and Sons Inc..
https://doi.org/10.1111/fcp.13036

Marinko M, Stojanović I, Milojević P, Nenezić D, Kanjuh V, Yang Q, He G, Novaković A. Involvement of different K+ channel subtypes in hydrogen sulfide-induced vasorelaxation of human internal mammary artery. in Fundamental and Clinical Pharmacology. 2024;.
doi:10.1111/fcp.13036 .

Marinko, Marija, Stojanović, Ivan, Milojević, Predrag, Nenezić, Dragoslav, Kanjuh, Vladimir, Yang, Qin, He, Guo-Wei, Novaković, Aleksandra, "Involvement of different K+ channel subtypes in hydrogen sulfide-induced vasorelaxation of human internal mammary artery" in Fundamental and Clinical Pharmacology (2024),
https://doi.org/10.1111/fcp.13036 . .

TY  - JOUR
AU  - Vekić, Jelena
AU  - Klisić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Milinković, Neda
AU  - Gluščević, Sanja
AU  - Ciftel, Serpi
AU  - Mercantepe, Filiz
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5751
AB  - Background: Subacute thyroiditis (SAT) is characterized by profound inflammation and fluctuations in thyroid hormones which may affect the hemostasis balance. This study investigates sex-specific associations between thyroid status, inflammation and hemostasis biomarkers in SAT. Methods: We included 52 patients (40 women and 12 men) treated with non-steroidal anti-inflammatory drugs (NSAID) or methylprednisolone (MPS). Free thyroxine (fT4), thyroid stimulating hormone, C-reactive protein, complete blood count and routine hemostasis parameters were assessed. Results: Both men and women were in hyperthyroid state and had comparable levels of inflammatory biomarkers. A shortened activated partial thromboplastin time (aPTT) was observed in 16.7% of the men and 10% of the women (p = 0.562), and a shortened prothrombin time (PT) was observed in 33% of the men and 12.5% of the women (p = 0.094). In men, aPTT positively correlated with fT4 (r = 0.627; p < 0.05), while PT positively correlated with leukocyte-based inflammatory indices in women (p < 0.05). NSAID-treated patients had lower aPTTs and platelet counts than those treated with MPS (p < 0.05). Principal component analysis extracted “proinflammatory”, “prothrombotic” and “antithrombotic” factors, but the “proinflammatory” factor was the independent predictor of elevated fT4 in women (OR = 2.705; p = 0.036). Conclusions: Our data demonstrated sex-specific associations of thyroid status and inflammatory biomarkers with hemostasis parameters in SAT. Routine hemostasis screening tests may help in monitoring the changes in the hemostasis system over the course of SAT.
PB  - MDPI
T2  - Biomedicines
T1  - Sex-Specific Associations between Thyroid Status, Inflammation and Hemostasis Biomarkers in Patients with Subacute Thyroiditis
VL  - 12
IS  - 8
DO  - 10.3390/biomedicines12081862
ER  - 

@article{
author = "Vekić, Jelena and Klisić, Aleksandra and Kotur-Stevuljević, Jelena and Milinković, Neda and Gluščević, Sanja and Ciftel, Serpi and Mercantepe, Filiz",
year = "2024",
abstract = "Background: Subacute thyroiditis (SAT) is characterized by profound inflammation and fluctuations in thyroid hormones which may affect the hemostasis balance. This study investigates sex-specific associations between thyroid status, inflammation and hemostasis biomarkers in SAT. Methods: We included 52 patients (40 women and 12 men) treated with non-steroidal anti-inflammatory drugs (NSAID) or methylprednisolone (MPS). Free thyroxine (fT4), thyroid stimulating hormone, C-reactive protein, complete blood count and routine hemostasis parameters were assessed. Results: Both men and women were in hyperthyroid state and had comparable levels of inflammatory biomarkers. A shortened activated partial thromboplastin time (aPTT) was observed in 16.7% of the men and 10% of the women (p = 0.562), and a shortened prothrombin time (PT) was observed in 33% of the men and 12.5% of the women (p = 0.094). In men, aPTT positively correlated with fT4 (r = 0.627; p < 0.05), while PT positively correlated with leukocyte-based inflammatory indices in women (p < 0.05). NSAID-treated patients had lower aPTTs and platelet counts than those treated with MPS (p < 0.05). Principal component analysis extracted “proinflammatory”, “prothrombotic” and “antithrombotic” factors, but the “proinflammatory” factor was the independent predictor of elevated fT4 in women (OR = 2.705; p = 0.036). Conclusions: Our data demonstrated sex-specific associations of thyroid status and inflammatory biomarkers with hemostasis parameters in SAT. Routine hemostasis screening tests may help in monitoring the changes in the hemostasis system over the course of SAT.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Sex-Specific Associations between Thyroid Status, Inflammation and Hemostasis Biomarkers in Patients with Subacute Thyroiditis",
volume = "12",
number = "8",
doi = "10.3390/biomedicines12081862"
}

Vekić, J., Klisić, A., Kotur-Stevuljević, J., Milinković, N., Gluščević, S., Ciftel, S.,& Mercantepe, F.. (2024). Sex-Specific Associations between Thyroid Status, Inflammation and Hemostasis Biomarkers in Patients with Subacute Thyroiditis. in Biomedicines
MDPI., 12(8).
https://doi.org/10.3390/biomedicines12081862

Vekić J, Klisić A, Kotur-Stevuljević J, Milinković N, Gluščević S, Ciftel S, Mercantepe F. Sex-Specific Associations between Thyroid Status, Inflammation and Hemostasis Biomarkers in Patients with Subacute Thyroiditis. in Biomedicines. 2024;12(8).
doi:10.3390/biomedicines12081862 .

Vekić, Jelena, Klisić, Aleksandra, Kotur-Stevuljević, Jelena, Milinković, Neda, Gluščević, Sanja, Ciftel, Serpi, Mercantepe, Filiz, "Sex-Specific Associations between Thyroid Status, Inflammation and Hemostasis Biomarkers in Patients with Subacute Thyroiditis" in Biomedicines, 12, no. 8 (2024),
https://doi.org/10.3390/biomedicines12081862 . .

TY  - JOUR
AU  - Milošević, Tamara
AU  - Naumović, Radomir
AU  - Sopić, Miron
AU  - Vekić, Jelena
AU  - Guzonjić, Azra
AU  - Pešić, Snežana
AU  - Miljković-Trailović, Milica
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5741
AB  - Background and objective: An increased risk of mortality and hospitalization was consistently demonstrated in hemodialysis (HD) patients affected by pandemic coronavirus infection (COVID-19). In this study, we analyzed parameters that may impact mortality in COVID-19 HD patients, including neutrophil-to-lymphocyte ratio (NLR), lactate dehydrogenase (LDH), C-reactive protein (CRP), COVID-19 disease status and telomere length in peripheral blood cells (TL). Materials and methods: A total of 130 chronic hemodialysis patients were enrolled and followed up for 18 months. Patients were categorized into groups based on their COVID-19 disease history and subsequent data about their survival status at the end of the study. Routine laboratory parameters were assessed using standard automated methods and TL was determined using the modified Cawthon method. Survival predictors were analyzed using Kaplan-Meier analysis. Results: Deceased patients (30%) were older with higher body mass index (BMI), higher levels of LDH, NLR index, CRP and lower TL and lymphocytes count compared to survivors. Kaplan-Meier survival analysis showed six parameters were significant mortality predictors in the following order of significance: COVID-19 history, 2-years cardiovascular mortality risk score, NLR, TL, CRP, LDH. Using binary logistic regression analysis Summary risk score, a combination of these six parameters revealed as the best predictor of patient’s survival in this group of parameters (log rank 25.4, p < 0.001). Conclusion: Compared to the general population, the mortality rate among HD patients persists at a higher level despite advancements in HD technology and patient care. The situation has been exacerbated by COVID-19, by significant increase in mortality rate among these patients.
PB  - Springer Nature
T2  - Molecular Biology Reports
T1  - COVID-19 increases mortality in hemodialysis patients: exploring links with inflammation and telomere attrition
VL  - 51
IS  - 1
DO  - 10.1007/s11033-024-09879-7
ER  - 

@article{
author = "Milošević, Tamara and Naumović, Radomir and Sopić, Miron and Vekić, Jelena and Guzonjić, Azra and Pešić, Snežana and Miljković-Trailović, Milica and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Background and objective: An increased risk of mortality and hospitalization was consistently demonstrated in hemodialysis (HD) patients affected by pandemic coronavirus infection (COVID-19). In this study, we analyzed parameters that may impact mortality in COVID-19 HD patients, including neutrophil-to-lymphocyte ratio (NLR), lactate dehydrogenase (LDH), C-reactive protein (CRP), COVID-19 disease status and telomere length in peripheral blood cells (TL). Materials and methods: A total of 130 chronic hemodialysis patients were enrolled and followed up for 18 months. Patients were categorized into groups based on their COVID-19 disease history and subsequent data about their survival status at the end of the study. Routine laboratory parameters were assessed using standard automated methods and TL was determined using the modified Cawthon method. Survival predictors were analyzed using Kaplan-Meier analysis. Results: Deceased patients (30%) were older with higher body mass index (BMI), higher levels of LDH, NLR index, CRP and lower TL and lymphocytes count compared to survivors. Kaplan-Meier survival analysis showed six parameters were significant mortality predictors in the following order of significance: COVID-19 history, 2-years cardiovascular mortality risk score, NLR, TL, CRP, LDH. Using binary logistic regression analysis Summary risk score, a combination of these six parameters revealed as the best predictor of patient’s survival in this group of parameters (log rank 25.4, p < 0.001). Conclusion: Compared to the general population, the mortality rate among HD patients persists at a higher level despite advancements in HD technology and patient care. The situation has been exacerbated by COVID-19, by significant increase in mortality rate among these patients.",
publisher = "Springer Nature",
journal = "Molecular Biology Reports",
title = "COVID-19 increases mortality in hemodialysis patients: exploring links with inflammation and telomere attrition",
volume = "51",
number = "1",
doi = "10.1007/s11033-024-09879-7"
}

Milošević, T., Naumović, R., Sopić, M., Vekić, J., Guzonjić, A., Pešić, S., Miljković-Trailović, M.,& Kotur-Stevuljević, J.. (2024). COVID-19 increases mortality in hemodialysis patients: exploring links with inflammation and telomere attrition. in Molecular Biology Reports
Springer Nature., 51(1).
https://doi.org/10.1007/s11033-024-09879-7

Milošević T, Naumović R, Sopić M, Vekić J, Guzonjić A, Pešić S, Miljković-Trailović M, Kotur-Stevuljević J. COVID-19 increases mortality in hemodialysis patients: exploring links with inflammation and telomere attrition. in Molecular Biology Reports. 2024;51(1).
doi:10.1007/s11033-024-09879-7 .

Milošević, Tamara, Naumović, Radomir, Sopić, Miron, Vekić, Jelena, Guzonjić, Azra, Pešić, Snežana, Miljković-Trailović, Milica, Kotur-Stevuljević, Jelena, "COVID-19 increases mortality in hemodialysis patients: exploring links with inflammation and telomere attrition" in Molecular Biology Reports, 51, no. 1 (2024),
https://doi.org/10.1007/s11033-024-09879-7 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Arsenović-Ranin, Nevena
AU  - Živković, Irena
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Dragačević, Luka
AU  - Kovačević, Ana
AU  - Kotur-Stevuljević, Jelena
AU  - Leposavić, Gordana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5721
AB  - Notwithstanding prevalence gaps in micronutrients supporting immune functions, the significance of their deficits/supplementation for the efficacy of vaccines is underinvestigated. Thus, the influence of supplementation combining vitamins C and D, zinc, selenium, manganese, and N-acetyl cysteine on immune correlates/surrogates of protection conferred by a quadrivalent influenza vaccine (QIV) in mice was investigated. The supplementation starting 5 days before the first of two QIV injections given 28 days apart increased the serum titres of total and neutralizing IgG against each of four influenza strains from QIV. Accordingly, the frequencies of germinal center B cells, follicular CD4+ T helper (Th) cells, and IL-21-producing Th cells increased in secondary lymphoid organs (SLOs). Additionally, the supplementation improved already increased IgG response to the second QIV injection by augmenting not only neutralizing antibody production, but also IgG2a response, which is important for virus clearance, through favoring Th1 differentiation as indicated by Th1 (IFN-γ)/Th2 (IL-4) signature cytokine level ratio upon QIV restimulation in SLO cell cultures. This most likely partly reflected antioxidant action of the supplement as indicated by splenic redox status analyses. Thus, the study provides a solid scientific background for further research aimed at repurposing the use of this safe and inexpensive micronutrient combination to improve response to the influenza vaccine.
PB  - MDPI
T2  - Vaccines
T1  - Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation
VL  - 12
IS  - 7
DO  - 10.3390/vaccines12070743
ER  - 

@article{
author = "Bufan, Biljana and Arsenović-Ranin, Nevena and Živković, Irena and Ćuruvija, Ivana and Blagojević, Veljko and Dragačević, Luka and Kovačević, Ana and Kotur-Stevuljević, Jelena and Leposavić, Gordana",
year = "2024",
abstract = "Notwithstanding prevalence gaps in micronutrients supporting immune functions, the significance of their deficits/supplementation for the efficacy of vaccines is underinvestigated. Thus, the influence of supplementation combining vitamins C and D, zinc, selenium, manganese, and N-acetyl cysteine on immune correlates/surrogates of protection conferred by a quadrivalent influenza vaccine (QIV) in mice was investigated. The supplementation starting 5 days before the first of two QIV injections given 28 days apart increased the serum titres of total and neutralizing IgG against each of four influenza strains from QIV. Accordingly, the frequencies of germinal center B cells, follicular CD4+ T helper (Th) cells, and IL-21-producing Th cells increased in secondary lymphoid organs (SLOs). Additionally, the supplementation improved already increased IgG response to the second QIV injection by augmenting not only neutralizing antibody production, but also IgG2a response, which is important for virus clearance, through favoring Th1 differentiation as indicated by Th1 (IFN-γ)/Th2 (IL-4) signature cytokine level ratio upon QIV restimulation in SLO cell cultures. This most likely partly reflected antioxidant action of the supplement as indicated by splenic redox status analyses. Thus, the study provides a solid scientific background for further research aimed at repurposing the use of this safe and inexpensive micronutrient combination to improve response to the influenza vaccine.",
publisher = "MDPI",
journal = "Vaccines",
title = "Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation",
volume = "12",
number = "7",
doi = "10.3390/vaccines12070743"
}

Bufan, B., Arsenović-Ranin, N., Živković, I., Ćuruvija, I., Blagojević, V., Dragačević, L., Kovačević, A., Kotur-Stevuljević, J.,& Leposavić, G.. (2024). Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation. in Vaccines
MDPI., 12(7).
https://doi.org/10.3390/vaccines12070743

Bufan B, Arsenović-Ranin N, Živković I, Ćuruvija I, Blagojević V, Dragačević L, Kovačević A, Kotur-Stevuljević J, Leposavić G. Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation. in Vaccines. 2024;12(7).
doi:10.3390/vaccines12070743 .

Bufan, Biljana, Arsenović-Ranin, Nevena, Živković, Irena, Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Kovačević, Ana, Kotur-Stevuljević, Jelena, Leposavić, Gordana, "Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation" in Vaccines, 12, no. 7 (2024),
https://doi.org/10.3390/vaccines12070743 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Bogavac-Stanojević, Nataša
AU  - Vekić, Jelena
AU  - Jovičić, Snežana
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5714
AB  - Until now, a lot of research has been carried out which significantly helped in
understanding and solving the problem of obesity. Despite this, there has been an upward in
obesity trend at the global level. The role of laboratory diagnostics in the field of obesity is of
great importance to doctors for establishing a diagnosis and monitoring the effects of therapy.
Determining biochemical parameters also contributes to practical usefulness in the prevention of
this disease, and prevention of consequent complications. Routinely available biochemical
analyses are usually used to diagnose and monitor the effects of obesity therapy. The initial
association related to laboratory analyses refers to parameters of lipid status, but there are a variety
of routine laboratory parameters that can help in understanding and monitoring obesity from
different angles. Timely and adequate management of obesity is also of interest from an economic
aspect. For this reason, the topic of this research is to summarize the latest aspects of the concept
of obesity, specifically from the point of view of biochemistry and laboratory diagnostics. Taking
into account the complexity of this disease, it is important to point out the future perspectives and
challenges that inevitably arise before both laboratory professionals and healthcare providers in
general.
AB  - Do sada je sprovedeno dosta istraživanja koja su značajno pomogla u sagledavanju i rešavanju problema gojaznosti. I pored toga, gojaznost ima trend porasta na globalnom nivou. Uloga laboratorijske dijagnostike u oblasti gojaznosti je od velikog značaja lekarima za postavljanje dijagnoze i praćenje efekata terapije. Određivanje biohemijskih parametara takođe doprinosi praktičnoj korisnosti u prevenciji ovog oboljenja, kao i predupređenju posledičnih komplikacija. Obično se za postavljanje dijagnoze i praćenje efekata terapije gojaznosti određuju rutinski dostupne biohemijske analize. Prvobitna asocijacija vezana za laboratorijske analize odnosi se na parametre lipidnog statusa, ali jako je veliki broj rutinskih analiza koje mogu pomoći u sagledavanju i praćenju gojaznosti iz različitih uglova. Dodatno, pravovremeno i adekvatno upravljanje gojaznošću je od interesa i sa ekonomskog aspekta. Iz tog razloga, tema ovog istraživanja je sumiranje najnovijih aspekata koncepta gojaznosti, posebno sa stanovišta biohemije i laboratorijske dijagnostike. Uzimajući u obzir složenost ove bolesti, važno je ukazati na buduće perspektive i izazove koji se neizbežno javljaju kako pred laboratorijskim stručnjacima, tako i pred zdravstvenim radnicima uopšte.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet
T2  - Arhiv za farmaciju
T1  - Biochemistry and laboratory diagnosis of obesity
T1  - Biohemija i laboratorijska dijagnostika gojaznosti
VL  - 74
IS  - 3
SP  - 348
EP  - 374
DO  - 10.5937/arhfarm74-50458
ER  - 

@article{
author = "Milinković, Neda and Bogavac-Stanojević, Nataša and Vekić, Jelena and Jovičić, Snežana and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Until now, a lot of research has been carried out which significantly helped in
understanding and solving the problem of obesity. Despite this, there has been an upward in
obesity trend at the global level. The role of laboratory diagnostics in the field of obesity is of
great importance to doctors for establishing a diagnosis and monitoring the effects of therapy.
Determining biochemical parameters also contributes to practical usefulness in the prevention of
this disease, and prevention of consequent complications. Routinely available biochemical
analyses are usually used to diagnose and monitor the effects of obesity therapy. The initial
association related to laboratory analyses refers to parameters of lipid status, but there are a variety
of routine laboratory parameters that can help in understanding and monitoring obesity from
different angles. Timely and adequate management of obesity is also of interest from an economic
aspect. For this reason, the topic of this research is to summarize the latest aspects of the concept
of obesity, specifically from the point of view of biochemistry and laboratory diagnostics. Taking
into account the complexity of this disease, it is important to point out the future perspectives and
challenges that inevitably arise before both laboratory professionals and healthcare providers in
general., Do sada je sprovedeno dosta istraživanja koja su značajno pomogla u sagledavanju i rešavanju problema gojaznosti. I pored toga, gojaznost ima trend porasta na globalnom nivou. Uloga laboratorijske dijagnostike u oblasti gojaznosti je od velikog značaja lekarima za postavljanje dijagnoze i praćenje efekata terapije. Određivanje biohemijskih parametara takođe doprinosi praktičnoj korisnosti u prevenciji ovog oboljenja, kao i predupređenju posledičnih komplikacija. Obično se za postavljanje dijagnoze i praćenje efekata terapije gojaznosti određuju rutinski dostupne biohemijske analize. Prvobitna asocijacija vezana za laboratorijske analize odnosi se na parametre lipidnog statusa, ali jako je veliki broj rutinskih analiza koje mogu pomoći u sagledavanju i praćenju gojaznosti iz različitih uglova. Dodatno, pravovremeno i adekvatno upravljanje gojaznošću je od interesa i sa ekonomskog aspekta. Iz tog razloga, tema ovog istraživanja je sumiranje najnovijih aspekata koncepta gojaznosti, posebno sa stanovišta biohemije i laboratorijske dijagnostike. Uzimajući u obzir složenost ove bolesti, važno je ukazati na buduće perspektive i izazove koji se neizbežno javljaju kako pred laboratorijskim stručnjacima, tako i pred zdravstvenim radnicima uopšte.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet",
journal = "Arhiv za farmaciju",
title = "Biochemistry and laboratory diagnosis of obesity, Biohemija i laboratorijska dijagnostika gojaznosti",
volume = "74",
number = "3",
pages = "348-374",
doi = "10.5937/arhfarm74-50458"
}

Milinković, N., Bogavac-Stanojević, N., Vekić, J., Jovičić, S.,& Kotur-Stevuljević, J.. (2024). Biochemistry and laboratory diagnosis of obesity. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(3), 348-374.
https://doi.org/10.5937/arhfarm74-50458

Milinković N, Bogavac-Stanojević N, Vekić J, Jovičić S, Kotur-Stevuljević J. Biochemistry and laboratory diagnosis of obesity. in Arhiv za farmaciju. 2024;74(3):348-374.
doi:10.5937/arhfarm74-50458 .

Milinković, Neda, Bogavac-Stanojević, Nataša, Vekić, Jelena, Jovičić, Snežana, Kotur-Stevuljević, Jelena, "Biochemistry and laboratory diagnosis of obesity" in Arhiv za farmaciju, 74, no. 3 (2024):348-374,
https://doi.org/10.5937/arhfarm74-50458 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Munjas, Jelena
AU  - Kovačević, Smiljka
AU  - Guzonjić, Azra
AU  - Mitrović, Jadranka
AU  - Saso, Luciano
AU  - Đuričić, Ivana
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5654
AB  - Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Childhood obesity accelerates biological ageing: is oxidative stress a link?
DO  - 10.1017/S0007114524000898
ER  - 

@article{
author = "Subošić, Branko and Zdravković, Vera and Ješić, Maja and Munjas, Jelena and Kovačević, Smiljka and Guzonjić, Azra and Mitrović, Jadranka and Saso, Luciano and Đuričić, Ivana and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Childhood obesity accelerates biological ageing: is oxidative stress a link?",
doi = "10.1017/S0007114524000898"
}

Subošić, B., Zdravković, V., Ješić, M., Munjas, J., Kovačević, S., Guzonjić, A., Mitrović, J., Saso, L., Đuričić, I.,& Kotur-Stevuljević, J.. (2024). Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition
Cambridge University Press..
https://doi.org/10.1017/S0007114524000898

Subošić B, Zdravković V, Ješić M, Munjas J, Kovačević S, Guzonjić A, Mitrović J, Saso L, Đuričić I, Kotur-Stevuljević J. Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition. 2024;.
doi:10.1017/S0007114524000898 .

Subošić, Branko, Zdravković, Vera, Ješić, Maja, Munjas, Jelena, Kovačević, Smiljka, Guzonjić, Azra, Mitrović, Jadranka, Saso, Luciano, Đuričić, Ivana, Kotur-Stevuljević, Jelena, "Childhood obesity accelerates biological ageing: is oxidative stress a link?" in British Journal of Nutrition (2024),
https://doi.org/10.1017/S0007114524000898 . .

TY  - JOUR
AU  - Simić, Ivana
AU  - Guzonjić, Azra
AU  - Kotur-Stevuljević, Jelena
AU  - Ćeriman Krstić, Vesna
AU  - Samardžić, Natalija
AU  - Savić Vujović, Katarina
AU  - Jovanović, Dragana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5648
AB  - Background and objectives: The objective of this research was to analyze the correlation of the neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), soluble programmed cell death ligand 1 (sPD-L1), and Schlafen 11 (SLFN11) with the response to first-line chemotherapy in a cohort of small cell lung cancer (SCLC) patients, and to determine their potential as predictive serum biomarkers. Materials and Methods: A total of 60 SCLC patients were included. Blood samples were taken to determine CRP, sPD-L1, and SLFN11 levels. The first sampling was performed before the start of chemotherapy, the second after two cycles, and the third after four cycles of chemotherapy. Results: The patients who died earlier during the study had NLR and SLFN11 concentrations significantly higher compared to the survivor group. In the group of survivors, after two cycles of chemotherapy, the NLR ratio decreased significantly (p < 0.01), but after four cycles, the NLR ratio increased (p < 0.05). Their serum SLFN11 concentration increased significantly (p < 0.001) after two cycles of chemotherapy, but after four cycles, the level of SLFN11 fell significantly (p < 0.01). CRP, NLR, and SLFN11 were significant predictors of patient survival according to Kaplan–Meier analysis. The combination of inflammatory parameters and SLFN11 with a cutoff value above the 75th percentile of the predicted probability was associated with significantly lower overall survival in SCLC patients (average survival of 3.6 months vs. 4.8 months). Conclusion: The combination of inflammatory markers and the levels of two specific proteins (sPD-L1, SLFN11) could potentially serve as a non-invasive biomarker for predicting responses to DNA-damaging therapeutic agents in SCLC.
PB  - MDPI
T2  - Biomedicines
T1  - Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study
VL  - 12
IS  - 5
DO  - 10.3390/biomedicines12050976
ER  - 

@article{
author = "Simić, Ivana and Guzonjić, Azra and Kotur-Stevuljević, Jelena and Ćeriman Krstić, Vesna and Samardžić, Natalija and Savić Vujović, Katarina and Jovanović, Dragana",
year = "2024",
abstract = "Background and objectives: The objective of this research was to analyze the correlation of the neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), soluble programmed cell death ligand 1 (sPD-L1), and Schlafen 11 (SLFN11) with the response to first-line chemotherapy in a cohort of small cell lung cancer (SCLC) patients, and to determine their potential as predictive serum biomarkers. Materials and Methods: A total of 60 SCLC patients were included. Blood samples were taken to determine CRP, sPD-L1, and SLFN11 levels. The first sampling was performed before the start of chemotherapy, the second after two cycles, and the third after four cycles of chemotherapy. Results: The patients who died earlier during the study had NLR and SLFN11 concentrations significantly higher compared to the survivor group. In the group of survivors, after two cycles of chemotherapy, the NLR ratio decreased significantly (p < 0.01), but after four cycles, the NLR ratio increased (p < 0.05). Their serum SLFN11 concentration increased significantly (p < 0.001) after two cycles of chemotherapy, but after four cycles, the level of SLFN11 fell significantly (p < 0.01). CRP, NLR, and SLFN11 were significant predictors of patient survival according to Kaplan–Meier analysis. The combination of inflammatory parameters and SLFN11 with a cutoff value above the 75th percentile of the predicted probability was associated with significantly lower overall survival in SCLC patients (average survival of 3.6 months vs. 4.8 months). Conclusion: The combination of inflammatory markers and the levels of two specific proteins (sPD-L1, SLFN11) could potentially serve as a non-invasive biomarker for predicting responses to DNA-damaging therapeutic agents in SCLC.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study",
volume = "12",
number = "5",
doi = "10.3390/biomedicines12050976"
}

Simić, I., Guzonjić, A., Kotur-Stevuljević, J., Ćeriman Krstić, V., Samardžić, N., Savić Vujović, K.,& Jovanović, D.. (2024). Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines
MDPI., 12(5).
https://doi.org/10.3390/biomedicines12050976

Simić I, Guzonjić A, Kotur-Stevuljević J, Ćeriman Krstić V, Samardžić N, Savić Vujović K, Jovanović D. Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines. 2024;12(5).
doi:10.3390/biomedicines12050976 .

Simić, Ivana, Guzonjić, Azra, Kotur-Stevuljević, Jelena, Ćeriman Krstić, Vesna, Samardžić, Natalija, Savić Vujović, Katarina, Jovanović, Dragana, "Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study" in Biomedicines, 12, no. 5 (2024),
https://doi.org/10.3390/biomedicines12050976 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
year = "2024",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I.. (2024). Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines. 2024;12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4 (2024),
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Živančević, Katarina
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Marić, Đurđica
AU  - Kotur-Stevuljević, Jelena
AU  - Ivanišević, Jasmina
AU  - Savić, Miroslav
AU  - Batinić, Bojan
AU  - Janković, Radmila
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5614
AB  - Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.
PB  - Elsevier Inc.
T2  - Environmental Research
T1  - Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach
VL  - 252
IS  - 1
SP  - 118680
DO  - 10.1016/j.envres.2024.118680
ER  - 

@article{
author = "Živančević, Katarina and Baralić, Katarina and Vukelić, Dragana and Marić, Đurđica and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Savić, Miroslav and Batinić, Bojan and Janković, Radmila and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.",
publisher = "Elsevier Inc.",
journal = "Environmental Research",
title = "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach",
volume = "252",
number = "1",
pages = "118680",
doi = "10.1016/j.envres.2024.118680"
}

Živančević, K., Baralić, K., Vukelić, D., Marić, Đ., Kotur-Stevuljević, J., Ivanišević, J., Savić, M., Batinić, B., Janković, R., Buha-Đorđević, A., Antonijević-Miljaković, E., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research
Elsevier Inc.., 252(1), 118680.
https://doi.org/10.1016/j.envres.2024.118680

Živančević K, Baralić K, Vukelić D, Marić Đ, Kotur-Stevuljević J, Ivanišević J, Savić M, Batinić B, Janković R, Buha-Đorđević A, Antonijević-Miljaković E, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research. 2024;252(1):118680.
doi:10.1016/j.envres.2024.118680 .

Živančević, Katarina, Baralić, Katarina, Vukelić, Dragana, Marić, Đurđica, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Savić, Miroslav, Batinić, Bojan, Janković, Radmila, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach" in Environmental Research, 252, no. 1 (2024):118680,
https://doi.org/10.1016/j.envres.2024.118680 . .

TY  - JOUR
AU  - Umićević, Nina
AU  - Kotur-Stevuljević, Jelena
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5592
AB  - This study aimed to assess the redox status and trace metal levels in 49 shoe industry workers (11 men and 38 women) occupationally exposed to a mixture of volatile organic compounds (VOCs), which includes aliphatic hydrocarbons, aromatic hydrocarbons, ketones, esters, ethers, and carboxylic acids. All measured VOCs were below the permitted occupational exposure limits. The control group included 50 unexposed participants (25 men and 25 women). The following plasma parameters were analysed: superoxide anion (O2•-), advanced oxidation protein products (AOPP), total oxidative status (TOS), prooxidant-antioxidant balance (PAB), oxidative stress index (OSI), superoxide dismutase (SOD) and paraoxonase-1 (PON1) enzyme activity, total SH group content (SHG), and total antioxidant status (TAS). Trace metal levels (copper, zinc, iron, magnesium, and manganese) were analysed in whole blood. All oxidative stress and antioxidative defence parameters were higher in the exposed workers than controls, except for PON1 activity. Higher Fe, Mg, and Zn, and lower Cu were observed in the exposed vs control men, while the exposed women had higher Fe and lower Mg, Zn, and Cu than their controls. Our findings confirm that combined exposure to a mixture of VOCs, even at permitted levels, may result in additive or synergistic adverse health effects and related disorders. This raises concern about current risk assessments, which mainly rely on the effects of individual chemicals, and calls for risk assessment approaches that can explain combined exposure to multiple chemicals.
AB  - Cilj istraživanja bio je procijeniti redoks status i koncentracije metala u tragovima kod 49 radnika (11 muškaraca i 38 žena) zaposlenih u
industriji obuće, izloženih mješavini hlapljivih organskih spojeva (engl. volatile organic compounds, krat. VOC) koju su činili alifatski i aromatični
ugljikovodici, ketoni, esteri, eteri i karboksilne kiseline. Sve izmjerene razine VOC bile su ispod dozvoljenih granica profesionalne izloženosti.
U kontrolnoj je skupini bilo 50 neizloženih ispitanika (25 muškaraca i 25 žena). U plazmi obućarskih radnika analizirani su sljedeći parametri:
superoksid anion radikal (O2
•-
), uznapredovali produkti oksidacije proteina (AOPP), totalni oksidacijski status (TOS), prooksidacijsko-
antioksidacijski balans (PAB), indeks oksidacijskoga stresa (OSI), aktivnost superoksid-dismutaze (SOD), aktivnost enzima paraoksonaze-1
(PON1), ukupni sadržaj sulfhidrilnih grupa (SHG) i totalni antioksidacijski status (TAS). Koncentracije metala u tragovima (bakar, cink,
željezo, magnezij i mangan) analizirane su u punoj krvi. U usporedbi s kontrolnom skupinom, svi parametri oksidacijskoga stresa i
antioksidacijske obrane u izloženih radnika bili su povišeni, osim aktivnosti PON1. Povećana koncentracija Fe, Mg i Zn te smanjena
koncentracija Cu uočene su u izloženih muškaraca u usporedbi s kontrolnima, a u žena je primijećena povišena koncentracija Fe, a niža
koncentracija Mg, Zn i Cu u odnosu na kontrolnu skupinu. Naši nalazi potvrđuju da kombinirano izlaganje mješavini VOC, čak i pri
dozvoljenim koncentracijama, može dovesti do aditivnih ili sinergističkih štetnih učinaka na zdravlje i popratnih poremećaja. Trenutna
procjena rizika, koja se uglavnom oslanja na učinke pojedinačnih kemikalija, izaziva zabrinutost i upućuje na potrebu za novim pristupima
koji će objasniti kombiniranu izloženost većem broju kemikalija.
PB  - Sciendo
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds
T1  - Povećani oksidacijski stres u obućarskih radnika izloženih mješavini niskih razina hlapljivih organskih spojeva
VL  - 75
IS  - 1
SP  - 51
EP  - 60
DO  - 10.2478/aiht-2024-75-3804
ER  - 

@article{
author = "Umićević, Nina and Kotur-Stevuljević, Jelena and Baralić, Katarina and Đukić-Ćosić, Danijela and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana",
year = "2024",
abstract = "This study aimed to assess the redox status and trace metal levels in 49 shoe industry workers (11 men and 38 women) occupationally exposed to a mixture of volatile organic compounds (VOCs), which includes aliphatic hydrocarbons, aromatic hydrocarbons, ketones, esters, ethers, and carboxylic acids. All measured VOCs were below the permitted occupational exposure limits. The control group included 50 unexposed participants (25 men and 25 women). The following plasma parameters were analysed: superoxide anion (O2•-), advanced oxidation protein products (AOPP), total oxidative status (TOS), prooxidant-antioxidant balance (PAB), oxidative stress index (OSI), superoxide dismutase (SOD) and paraoxonase-1 (PON1) enzyme activity, total SH group content (SHG), and total antioxidant status (TAS). Trace metal levels (copper, zinc, iron, magnesium, and manganese) were analysed in whole blood. All oxidative stress and antioxidative defence parameters were higher in the exposed workers than controls, except for PON1 activity. Higher Fe, Mg, and Zn, and lower Cu were observed in the exposed vs control men, while the exposed women had higher Fe and lower Mg, Zn, and Cu than their controls. Our findings confirm that combined exposure to a mixture of VOCs, even at permitted levels, may result in additive or synergistic adverse health effects and related disorders. This raises concern about current risk assessments, which mainly rely on the effects of individual chemicals, and calls for risk assessment approaches that can explain combined exposure to multiple chemicals., Cilj istraživanja bio je procijeniti redoks status i koncentracije metala u tragovima kod 49 radnika (11 muškaraca i 38 žena) zaposlenih u
industriji obuće, izloženih mješavini hlapljivih organskih spojeva (engl. volatile organic compounds, krat. VOC) koju su činili alifatski i aromatični
ugljikovodici, ketoni, esteri, eteri i karboksilne kiseline. Sve izmjerene razine VOC bile su ispod dozvoljenih granica profesionalne izloženosti.
U kontrolnoj je skupini bilo 50 neizloženih ispitanika (25 muškaraca i 25 žena). U plazmi obućarskih radnika analizirani su sljedeći parametri:
superoksid anion radikal (O2
•-
), uznapredovali produkti oksidacije proteina (AOPP), totalni oksidacijski status (TOS), prooksidacijsko-
antioksidacijski balans (PAB), indeks oksidacijskoga stresa (OSI), aktivnost superoksid-dismutaze (SOD), aktivnost enzima paraoksonaze-1
(PON1), ukupni sadržaj sulfhidrilnih grupa (SHG) i totalni antioksidacijski status (TAS). Koncentracije metala u tragovima (bakar, cink,
željezo, magnezij i mangan) analizirane su u punoj krvi. U usporedbi s kontrolnom skupinom, svi parametri oksidacijskoga stresa i
antioksidacijske obrane u izloženih radnika bili su povišeni, osim aktivnosti PON1. Povećana koncentracija Fe, Mg i Zn te smanjena
koncentracija Cu uočene su u izloženih muškaraca u usporedbi s kontrolnima, a u žena je primijećena povišena koncentracija Fe, a niža
koncentracija Mg, Zn i Cu u odnosu na kontrolnu skupinu. Naši nalazi potvrđuju da kombinirano izlaganje mješavini VOC, čak i pri
dozvoljenim koncentracijama, može dovesti do aditivnih ili sinergističkih štetnih učinaka na zdravlje i popratnih poremećaja. Trenutna
procjena rizika, koja se uglavnom oslanja na učinke pojedinačnih kemikalija, izaziva zabrinutost i upućuje na potrebu za novim pristupima
koji će objasniti kombiniranu izloženost većem broju kemikalija.",
publisher = "Sciendo",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds, Povećani oksidacijski stres u obućarskih radnika izloženih mješavini niskih razina hlapljivih organskih spojeva",
volume = "75",
number = "1",
pages = "51-60",
doi = "10.2478/aiht-2024-75-3804"
}

Umićević, N., Kotur-Stevuljević, J., Baralić, K., Đukić-Ćosić, D., Antonijević-Miljaković, E., Buha-Đorđević, A., Ćurčić, M., Bulat, Z.,& Antonijević, B.. (2024). Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Sciendo., 75(1), 51-60.
https://doi.org/10.2478/aiht-2024-75-3804

Umićević N, Kotur-Stevuljević J, Baralić K, Đukić-Ćosić D, Antonijević-Miljaković E, Buha-Đorđević A, Ćurčić M, Bulat Z, Antonijević B. Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(1):51-60.
doi:10.2478/aiht-2024-75-3804 .

Umićević, Nina, Kotur-Stevuljević, Jelena, Baralić, Katarina, Đukić-Ćosić, Danijela, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, "Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 1 (2024):51-60,
https://doi.org/10.2478/aiht-2024-75-3804 . .

TY  - JOUR
AU  - Mirjanić-Azarić, Bosa
AU  - Stanković, Siniša
AU  - Radić Savić, Žana
AU  - Malčić-Žanić, Dragana
AU  - Ninić, Ana
AU  - Vuković, Marija
AU  - Nežić, Lana
AU  - Skrbić, Ranko
AU  - Bogavac-Stanojević, Nataša
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5758
AB  - Background: Recent findings point to the key role of
cathepsin S (CTSS) in the survival of malignant cells, as
well as the significance of the anti-apoptotic properties of
high-density lipoprotein (HDL) that contribute to enhanced
cell survival. The purpose of this study is to analyse CTSS
as a potential biomarker in lymphoma. Also, in order to
better understand the role of CTSS in the origin and devel-
opment of lymphoma, its association with cystatin C (Cys
C), lipids, and inflammatory markers was analysed.
Methods: The study included 90 subjects: 11 Hodgkin (HL)
and 44 B-cell non-Hodgkin lymphoma (NHL) patients, as
well as 35 healthy subjects. CTSS was determined using
the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc.,
Waltham, MA, USA). The non-denaturing 3%–31% poly-
acrylamide gradient gel electrophoresis method was used
to separate plasma HDL particles.
AB  - Uvod: Novija otkri}a ukazuju na klju~nu ulogu katepsina S
(CTSS) u pre`ivljavanju malignih }elija, kao i na zna~aj anti-
apoptoti~kih osobina lipoproteina visoke gusto}e (HDL)
koje doprinose ve}em pre`ivljavanja }elija. Cilj ove studije
je analizirati CTSS kao potencijalni biomarker kod limfomu.
Tako|e, kako bi se bolje razumela uloga CTSS-a u nastan-
ku i razvoju limfoma, analizirana je njegova povezanost sa
cistatinom C (Cys C), lipidima i upalnim markerima.
Metode: U istra`ivanje je bilo uklju~eno 90 ispitanika: 11
bolesnika sa Hodgkinovim (HL) i 44 bolesnika sa B-}elij-
skim ne-Hodgkinovim limfomom (NHL), te 35 zdravih ispi-
tanika. Za merenje serumskog CTSS je kori{}en Invitrogen
ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA,
SAD). HDL subfrakcije su razdvojene metodom vertikalne
elektroforeze na gradijentu poliakrilamida.
PB  - Društvo medicinskih biohemičara Srbije, Beograd
T2  - Journal of Medical Biochemistry
T1  - Assessment of the diagnostic value of serum cathepsin s and its correlation with hdl  ubclasses in patients with non-hodgkin’s lymphoma
T1  - Procena dijagnostičke vrednosti serumskog katepsina S i njegove korelacije sa HDL podklasama kod pacijenata sa ne-Hodgkinovim limfomom
VL  - 43
IS  - 5
SP  - 711
EP  - 719
DO  - 10.5937/jomb0-48959
ER  - 

@article{
author = "Mirjanić-Azarić, Bosa and Stanković, Siniša and Radić Savić, Žana and Malčić-Žanić, Dragana and Ninić, Ana and Vuković, Marija and Nežić, Lana and Skrbić, Ranko and Bogavac-Stanojević, Nataša",
year = "2024",
abstract = "Background: Recent findings point to the key role of
cathepsin S (CTSS) in the survival of malignant cells, as
well as the significance of the anti-apoptotic properties of
high-density lipoprotein (HDL) that contribute to enhanced
cell survival. The purpose of this study is to analyse CTSS
as a potential biomarker in lymphoma. Also, in order to
better understand the role of CTSS in the origin and devel-
opment of lymphoma, its association with cystatin C (Cys
C), lipids, and inflammatory markers was analysed.
Methods: The study included 90 subjects: 11 Hodgkin (HL)
and 44 B-cell non-Hodgkin lymphoma (NHL) patients, as
well as 35 healthy subjects. CTSS was determined using
the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc.,
Waltham, MA, USA). The non-denaturing 3%–31% poly-
acrylamide gradient gel electrophoresis method was used
to separate plasma HDL particles., Uvod: Novija otkri}a ukazuju na klju~nu ulogu katepsina S
(CTSS) u pre`ivljavanju malignih }elija, kao i na zna~aj anti-
apoptoti~kih osobina lipoproteina visoke gusto}e (HDL)
koje doprinose ve}em pre`ivljavanja }elija. Cilj ove studije
je analizirati CTSS kao potencijalni biomarker kod limfomu.
Tako|e, kako bi se bolje razumela uloga CTSS-a u nastan-
ku i razvoju limfoma, analizirana je njegova povezanost sa
cistatinom C (Cys C), lipidima i upalnim markerima.
Metode: U istra`ivanje je bilo uklju~eno 90 ispitanika: 11
bolesnika sa Hodgkinovim (HL) i 44 bolesnika sa B-}elij-
skim ne-Hodgkinovim limfomom (NHL), te 35 zdravih ispi-
tanika. Za merenje serumskog CTSS je kori{}en Invitrogen
ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA,
SAD). HDL subfrakcije su razdvojene metodom vertikalne
elektroforeze na gradijentu poliakrilamida.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd",
journal = "Journal of Medical Biochemistry",
title = "Assessment of the diagnostic value of serum cathepsin s and its correlation with hdl  ubclasses in patients with non-hodgkin’s lymphoma, Procena dijagnostičke vrednosti serumskog katepsina S i njegove korelacije sa HDL podklasama kod pacijenata sa ne-Hodgkinovim limfomom",
volume = "43",
number = "5",
pages = "711-719",
doi = "10.5937/jomb0-48959"
}

Mirjanić-Azarić, B., Stanković, S., Radić Savić, Ž., Malčić-Žanić, D., Ninić, A., Vuković, M., Nežić, L., Skrbić, R.,& Bogavac-Stanojević, N.. (2024). Assessment of the diagnostic value of serum cathepsin s and its correlation with hdl  ubclasses in patients with non-hodgkin’s lymphoma. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd., 43(5), 711-719.
https://doi.org/10.5937/jomb0-48959

Mirjanić-Azarić B, Stanković S, Radić Savić Ž, Malčić-Žanić D, Ninić A, Vuković M, Nežić L, Skrbić R, Bogavac-Stanojević N. Assessment of the diagnostic value of serum cathepsin s and its correlation with hdl  ubclasses in patients with non-hodgkin’s lymphoma. in Journal of Medical Biochemistry. 2024;43(5):711-719.
doi:10.5937/jomb0-48959 .

Mirjanić-Azarić, Bosa, Stanković, Siniša, Radić Savić, Žana, Malčić-Žanić, Dragana, Ninić, Ana, Vuković, Marija, Nežić, Lana, Skrbić, Ranko, Bogavac-Stanojević, Nataša, "Assessment of the diagnostic value of serum cathepsin s and its correlation with hdl  ubclasses in patients with non-hodgkin’s lymphoma" in Journal of Medical Biochemistry, 43, no. 5 (2024):711-719,
https://doi.org/10.5937/jomb0-48959 . .

TY  - JOUR
AU  - Skrede, Silje
AU  - Bogavac-Stanojević, Nataša
AU  - Dreesen, Erwin
AU  - Nielsen, Elisabet
AU  - Zaninotto, Martina
AU  - Mulleman, Denis
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5532
AB  - The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.
PB  - John Wiley and Sons Inc
T2  - Basic and Clinical Pharmacology and Toxicology
T1  - Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe
DO  - 10.1111/bcpt.13983
ER  - 

@article{
author = "Skrede, Silje and Bogavac-Stanojević, Nataša and Dreesen, Erwin and Nielsen, Elisabet and Zaninotto, Martina and Mulleman, Denis",
year = "2024",
abstract = "The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.",
publisher = "John Wiley and Sons Inc",
journal = "Basic and Clinical Pharmacology and Toxicology",
title = "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe",
doi = "10.1111/bcpt.13983"
}

Skrede, S., Bogavac-Stanojević, N., Dreesen, E., Nielsen, E., Zaninotto, M.,& Mulleman, D.. (2024). Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology
John Wiley and Sons Inc..
https://doi.org/10.1111/bcpt.13983

Skrede S, Bogavac-Stanojević N, Dreesen E, Nielsen E, Zaninotto M, Mulleman D. Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology. 2024;.
doi:10.1111/bcpt.13983 .

Skrede, Silje, Bogavac-Stanojević, Nataša, Dreesen, Erwin, Nielsen, Elisabet, Zaninotto, Martina, Mulleman, Denis, "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe" in Basic and Clinical Pharmacology and Toxicology (2024),
https://doi.org/10.1111/bcpt.13983 . .

Rupar, J., Marković, M., Božić, D., Antić-Stanković, J., Mijailović, N., Gavrilov, N., Bajuk-Bogdanović, D., Milojević-Rakić, M.,& Janošević-Ležaić, A.. (2024). Facile and Low-Cost Electrochemical Synthesis of Zinc
Alginate Hydrogel Films for Biomedical Applications. in Applied Organometallic Chemistry
John Wiley and Sons Ltd..
https://doi.org/10.1002/aoc.7732

Rupar J, Marković M, Božić D, Antić-Stanković J, Mijailović N, Gavrilov N, Bajuk-Bogdanović D, Milojević-Rakić M, Janošević-Ležaić A. Facile and Low-Cost Electrochemical Synthesis of Zinc
Alginate Hydrogel Films for Biomedical Applications. in Applied Organometallic Chemistry. 2024;.
doi:10.1002/aoc.7732 .

Rupar, Jelena, Marković, Mihajlo, Božić, Dragana, Antić-Stanković, Jelena, Mijailović, Nataša, Gavrilov, Nemanja, Bajuk-Bogdanović, Danica, Milojević-Rakić, Maja, Janošević-Ležaić, Aleksandra, "Facile and Low-Cost Electrochemical Synthesis of Zinc
Alginate Hydrogel Films for Biomedical Applications" in Applied Organometallic Chemistry (2024),
https://doi.org/10.1002/aoc.7732 . .

TY  - JOUR
AU  - Medarević, Đorđe
AU  - Čežek, Maša
AU  - Knežević, Aleksandar
AU  - Turković, Erna
AU  - Barudžija, Tanja
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5733
AB  - A lack of strategies for the utilization of harvest residues (HRs) has led to serious environmental problems due to an accumulation of these residues or their burning in the field. In this study, wheat and corn HRs were used as feedstock for the production of microcrystalline cellulose (MCC) by treatment with 2–8% sodium hydroxide, 10% hydrogen peroxide and further hydrolysis with 1–2 M hydrochloric acid. The changes in the FT-IR spectra and PXRD diffractograms after chemical treatment confirmed the removal of most of the lignin, hemicellulose and amorphous fraction of cellulose. A higher degree of crystallinity was observed for MCC obtained from corn HRs, which was attributed to a more efficient removal of lignin and hemicellulose by a higher sodium hydroxide concentration, which facilitates the dissolution of amorphous cellulose during acid hydrolysis. MCC obtained from HRs exhibited lower bulk density and poorer flow properties but similar or better tableting properties compared to commercial MCC (CeolusTM PH101). The lower ejection and detachment stress suggests that MCC isolated from HRs requires less lubricant compared to commercial MCC. This study showed that MCC isolated from wheat and corn HRs exhibits comparable tableting behaviour like commercial sample, further supporting this type of agricultural waste utilization.
PB  - MDPI
T2  - Pharmaceutics
T1  - From Field to Pharmacy: Isolation, Characterization and Tableting Behaviour of Microcrystalline Cellulose from Wheat and Corn Harvest Residues
VL  - 16
IS  - 8
SP  - 1090
DO  - 10.3390/pharmaceutics16081090
ER  - 

@article{
author = "Medarević, Đorđe and Čežek, Maša and Knežević, Aleksandar and Turković, Erna and Barudžija, Tanja and Samardžić, Stevan and Maksimović, Zoran",
year = "2024",
abstract = "A lack of strategies for the utilization of harvest residues (HRs) has led to serious environmental problems due to an accumulation of these residues or their burning in the field. In this study, wheat and corn HRs were used as feedstock for the production of microcrystalline cellulose (MCC) by treatment with 2–8% sodium hydroxide, 10% hydrogen peroxide and further hydrolysis with 1–2 M hydrochloric acid. The changes in the FT-IR spectra and PXRD diffractograms after chemical treatment confirmed the removal of most of the lignin, hemicellulose and amorphous fraction of cellulose. A higher degree of crystallinity was observed for MCC obtained from corn HRs, which was attributed to a more efficient removal of lignin and hemicellulose by a higher sodium hydroxide concentration, which facilitates the dissolution of amorphous cellulose during acid hydrolysis. MCC obtained from HRs exhibited lower bulk density and poorer flow properties but similar or better tableting properties compared to commercial MCC (CeolusTM PH101). The lower ejection and detachment stress suggests that MCC isolated from HRs requires less lubricant compared to commercial MCC. This study showed that MCC isolated from wheat and corn HRs exhibits comparable tableting behaviour like commercial sample, further supporting this type of agricultural waste utilization.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "From Field to Pharmacy: Isolation, Characterization and Tableting Behaviour of Microcrystalline Cellulose from Wheat and Corn Harvest Residues",
volume = "16",
number = "8",
pages = "1090",
doi = "10.3390/pharmaceutics16081090"
}

Medarević, Đ., Čežek, M., Knežević, A., Turković, E., Barudžija, T., Samardžić, S.,& Maksimović, Z.. (2024). From Field to Pharmacy: Isolation, Characterization and Tableting Behaviour of Microcrystalline Cellulose from Wheat and Corn Harvest Residues. in Pharmaceutics
MDPI., 16(8), 1090.
https://doi.org/10.3390/pharmaceutics16081090

Medarević Đ, Čežek M, Knežević A, Turković E, Barudžija T, Samardžić S, Maksimović Z. From Field to Pharmacy: Isolation, Characterization and Tableting Behaviour of Microcrystalline Cellulose from Wheat and Corn Harvest Residues. in Pharmaceutics. 2024;16(8):1090.
doi:10.3390/pharmaceutics16081090 .

Medarević, Đorđe, Čežek, Maša, Knežević, Aleksandar, Turković, Erna, Barudžija, Tanja, Samardžić, Stevan, Maksimović, Zoran, "From Field to Pharmacy: Isolation, Characterization and Tableting Behaviour of Microcrystalline Cellulose from Wheat and Corn Harvest Residues" in Pharmaceutics, 16, no. 8 (2024):1090,
https://doi.org/10.3390/pharmaceutics16081090 . .

TY  - JOUR
AU  - Toljić, Mina
AU  - Nikolić, Nađa
AU  - Joksić, Ivana
AU  - Čarkić, Jelena
AU  - Munjas, Jelena
AU  - Karadžov Orlić, Nataša
AU  - Milašin, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5615
AB  - Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.
PB  - Elsevier B.V.
T2  - Journal of Reproductive Immunology
T1  - Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus
VL  - 162
SP  - 104211
DO  - 10.1016/j.jri.2024.104211
ER  - 

@article{
author = "Toljić, Mina and Nikolić, Nađa and Joksić, Ivana and Čarkić, Jelena and Munjas, Jelena and Karadžov Orlić, Nataša and Milašin, Jelena",
year = "2024",
abstract = "Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.",
publisher = "Elsevier B.V.",
journal = "Journal of Reproductive Immunology",
title = "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus",
volume = "162",
pages = "104211",
doi = "10.1016/j.jri.2024.104211"
}

Toljić, M., Nikolić, N., Joksić, I., Čarkić, J., Munjas, J., Karadžov Orlić, N.,& Milašin, J.. (2024). Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology
Elsevier B.V.., 162, 104211.
https://doi.org/10.1016/j.jri.2024.104211

Toljić M, Nikolić N, Joksić I, Čarkić J, Munjas J, Karadžov Orlić N, Milašin J. Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology. 2024;162:104211.
doi:10.1016/j.jri.2024.104211 .

Toljić, Mina, Nikolić, Nađa, Joksić, Ivana, Čarkić, Jelena, Munjas, Jelena, Karadžov Orlić, Nataša, Milašin, Jelena, "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus" in Journal of Reproductive Immunology, 162 (2024):104211,
https://doi.org/10.1016/j.jri.2024.104211 . .

TY  - JOUR
AU  - Rafaqat, Saira
AU  - Sharif, Saima
AU  - Naz, Shagufta
AU  - Gluščević, Sanja
AU  - Mercantepe, Mercantepe
AU  - Ninić, Ana
AU  - Klisić, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5756
AB  - Background and Objectives: This study aimed to examine the relationship between cardiometabolic risk factors and atrial fibrillation (AF) and the simultaneous presence of AF and metabolic syndrome (MetS) in the Pakistani population. Materials and Methods: A total of 690 subjects were enrolled (n = 230 patients with AF, n = 230 patients with AF and MetS, and n = 230 controls). The associations between cardiometabolic parameters and AF with and without MetS were analyzed by univariable and multivariable binary regression analyses. Results: Body mass index (BMI), fasting blood glucose (FBG), and triglycerides (TG) were independently positively correlated, but the glomerular filtration rate (GFR) and sodium were independently negatively correlated with AF. An increase in BMI, FBG, and TG levels by one unit measure increased the probability by 55.1%, 20.6%, and 1.3%, respectively, for the AF occurrence. A decrease in GFR and sodium levels increased the probability by 4.3% and 33.6%, respectively, for the AF occurrence. On the other hand, uric acid was independently negatively correlated, whereas sodium was independently positively correlated, with MetS and AF. A decrease in uric acid levels and an increase in sodium levels by 1 unit measure increased the probability for MetS and AF by 23.2% and 7.5%, respectively. Conclusions: Cost-effective and routinely measured parameters, i.e., BMI, FBG TG, GFR, and sodium levels, can be reliable indicators of AF, whereas serum uric acid and sodium levels are independently associated with AF and MetS in the Pakistani population. Timely recognition and the control of modifiable cardiometabolic risk factors are of great significance in the prevention of AF development.
PB  - MDPI
T2  - Medicina (Lithuania)
T1  - Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population
VL  - 60
IS  - 8
DO  - 10.3390/medicina60081190
ER  - 

@article{
author = "Rafaqat, Saira and Sharif, Saima and Naz, Shagufta and Gluščević, Sanja and Mercantepe, Mercantepe and Ninić, Ana and Klisić, Aleksandra",
year = "2024",
abstract = "Background and Objectives: This study aimed to examine the relationship between cardiometabolic risk factors and atrial fibrillation (AF) and the simultaneous presence of AF and metabolic syndrome (MetS) in the Pakistani population. Materials and Methods: A total of 690 subjects were enrolled (n = 230 patients with AF, n = 230 patients with AF and MetS, and n = 230 controls). The associations between cardiometabolic parameters and AF with and without MetS were analyzed by univariable and multivariable binary regression analyses. Results: Body mass index (BMI), fasting blood glucose (FBG), and triglycerides (TG) were independently positively correlated, but the glomerular filtration rate (GFR) and sodium were independently negatively correlated with AF. An increase in BMI, FBG, and TG levels by one unit measure increased the probability by 55.1%, 20.6%, and 1.3%, respectively, for the AF occurrence. A decrease in GFR and sodium levels increased the probability by 4.3% and 33.6%, respectively, for the AF occurrence. On the other hand, uric acid was independently negatively correlated, whereas sodium was independently positively correlated, with MetS and AF. A decrease in uric acid levels and an increase in sodium levels by 1 unit measure increased the probability for MetS and AF by 23.2% and 7.5%, respectively. Conclusions: Cost-effective and routinely measured parameters, i.e., BMI, FBG TG, GFR, and sodium levels, can be reliable indicators of AF, whereas serum uric acid and sodium levels are independently associated with AF and MetS in the Pakistani population. Timely recognition and the control of modifiable cardiometabolic risk factors are of great significance in the prevention of AF development.",
publisher = "MDPI",
journal = "Medicina (Lithuania)",
title = "Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population",
volume = "60",
number = "8",
doi = "10.3390/medicina60081190"
}

Rafaqat, S., Sharif, S., Naz, S., Gluščević, S., Mercantepe, M., Ninić, A.,& Klisić, A.. (2024). Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population. in Medicina (Lithuania)
MDPI., 60(8).
https://doi.org/10.3390/medicina60081190

Rafaqat S, Sharif S, Naz S, Gluščević S, Mercantepe M, Ninić A, Klisić A. Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population. in Medicina (Lithuania). 2024;60(8).
doi:10.3390/medicina60081190 .

Rafaqat, Saira, Sharif, Saima, Naz, Shagufta, Gluščević, Sanja, Mercantepe, Mercantepe, Ninić, Ana, Klisić, Aleksandra, "Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population" in Medicina (Lithuania), 60, no. 8 (2024),
https://doi.org/10.3390/medicina60081190 . .

TY  - JOUR
AU  - Franković, Irena
AU  - Đuričić, Ivana
AU  - Ninić, Ana
AU  - Vekić, Jelena
AU  - Vorkapić, Tara
AU  - Erceg, Sanja
AU  - Gojković, Tamara
AU  - Tomašević, Ratko
AU  - Mamić, Milica
AU  - Mitrović, Miloš
AU  - Zeljković, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5755
AB  - The increasing prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) underscores the need for better understanding of its complex pathogenesis. Lipid accumulation in hepatocytes is among principal mechanisms contributing to MASLD development. While routine lipid parameters are well studied, the profile of circulating fatty acids in MASLD patients remains less explored. This study aimed to assess relative proportions of individual fatty acids in plasma of MASLD patients and to explore their associations with other biochemical markers of MASLD. Ninety-one patients and 48 healthy individuals were enrolled. The relative proportions of fatty acids in plasma were determined using gas chromatography with FID detection. Proportions of total n-6 polyunsaturated fatty acids (PUFAs) and linoleic acid (LA) in plasma were lower in MASLD patients (p = 0.001 and p = 0.004, respectively), with no differences observed in n-3 PUFAs. Total plasma n-6 PUFAs correlated negatively with body mass index, hepatic steatosis indices, triglyceride concentration and coronary risk index. Decreased prevalence of n-6 PUFAs in plasma was independently associated with higher odds of MASLD (OR = 0.769; CI: 0.611–0.968; p = 0.025). Our findings indicate an altered circulatory fatty acid distribution in MASLD, characterized by a reduced amount of n-6 PUFAs, particularly LA, which may have significant implications for the prevention and treatment of MASLD.
PB  - MDPI
T2  - Biomolecules
T1  - Increased Odds of Metabolic Dysfunction-Associated Steatotic
Liver Disease Are Linked to Reduced n-6, but Not n-3
Polyunsaturated Fatty Acids in Plasma
VL  - 14
IS  - 8
DO  - 10.3390/biom14080902
ER  - 

@article{
author = "Franković, Irena and Đuričić, Ivana and Ninić, Ana and Vekić, Jelena and Vorkapić, Tara and Erceg, Sanja and Gojković, Tamara and Tomašević, Ratko and Mamić, Milica and Mitrović, Miloš and Zeljković, Aleksandra",
year = "2024",
abstract = "The increasing prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) underscores the need for better understanding of its complex pathogenesis. Lipid accumulation in hepatocytes is among principal mechanisms contributing to MASLD development. While routine lipid parameters are well studied, the profile of circulating fatty acids in MASLD patients remains less explored. This study aimed to assess relative proportions of individual fatty acids in plasma of MASLD patients and to explore their associations with other biochemical markers of MASLD. Ninety-one patients and 48 healthy individuals were enrolled. The relative proportions of fatty acids in plasma were determined using gas chromatography with FID detection. Proportions of total n-6 polyunsaturated fatty acids (PUFAs) and linoleic acid (LA) in plasma were lower in MASLD patients (p = 0.001 and p = 0.004, respectively), with no differences observed in n-3 PUFAs. Total plasma n-6 PUFAs correlated negatively with body mass index, hepatic steatosis indices, triglyceride concentration and coronary risk index. Decreased prevalence of n-6 PUFAs in plasma was independently associated with higher odds of MASLD (OR = 0.769; CI: 0.611–0.968; p = 0.025). Our findings indicate an altered circulatory fatty acid distribution in MASLD, characterized by a reduced amount of n-6 PUFAs, particularly LA, which may have significant implications for the prevention and treatment of MASLD.",
publisher = "MDPI",
journal = "Biomolecules",
title = "Increased Odds of Metabolic Dysfunction-Associated Steatotic
Liver Disease Are Linked to Reduced n-6, but Not n-3
Polyunsaturated Fatty Acids in Plasma",
volume = "14",
number = "8",
doi = "10.3390/biom14080902"
}

Franković, I., Đuričić, I., Ninić, A., Vekić, J., Vorkapić, T., Erceg, S., Gojković, T., Tomašević, R., Mamić, M., Mitrović, M.,& Zeljković, A.. (2024). Increased Odds of Metabolic Dysfunction-Associated Steatotic
Liver Disease Are Linked to Reduced n-6, but Not n-3
Polyunsaturated Fatty Acids in Plasma. in Biomolecules
MDPI., 14(8).
https://doi.org/10.3390/biom14080902

Franković I, Đuričić I, Ninić A, Vekić J, Vorkapić T, Erceg S, Gojković T, Tomašević R, Mamić M, Mitrović M, Zeljković A. Increased Odds of Metabolic Dysfunction-Associated Steatotic
Liver Disease Are Linked to Reduced n-6, but Not n-3
Polyunsaturated Fatty Acids in Plasma. in Biomolecules. 2024;14(8).
doi:10.3390/biom14080902 .

Franković, Irena, Đuričić, Ivana, Ninić, Ana, Vekić, Jelena, Vorkapić, Tara, Erceg, Sanja, Gojković, Tamara, Tomašević, Ratko, Mamić, Milica, Mitrović, Miloš, Zeljković, Aleksandra, "Increased Odds of Metabolic Dysfunction-Associated Steatotic
Liver Disease Are Linked to Reduced n-6, but Not n-3
Polyunsaturated Fatty Acids in Plasma" in Biomolecules, 14, no. 8 (2024),
https://doi.org/10.3390/biom14080902 . .

TY  - JOUR
AU  - Anić-Marković, Bojana
AU  - Marinković, Aleksandar
AU  - Antić-Stanković, Jelena
AU  - Mijatović, Stefan
AU  - Cvijetić, Ilija
AU  - Simić, Milena
AU  - Aranđelović, Irena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5754
AB  - Antioxidants are promising compounds with antimicrobial activity against drug-resistant pathogens, especially when combined with conventional antimicrobials. Our study aimed to characterize the structure of nicotinamides synthesized from nicotinic acid and thiocarbohydrazones and to evaluate their antibacterial and antifungal activity. Seven nicotinic acid hydrazides (NC 1–7) were synthesized using mono-thiocarbohydrazones with hydroxyl group substituents, along with quinolone, phenolic, and pyridine rings known for their antimicrobial activity. The in vitro antimicrobial activity of NC 1–7, at concentrations ranging from 0.001 to 1 mM, was tested against Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (NCIMB 9111), and Candida albicans (ATCC 24433) using the broth microdilution method per EUCAST 2024 guidelines. Microorganism survival percentages were calculated based on optical density, and target fishing using the PharmMapper database identified potential molecular targets. The results showed that P. aeruginosa was most susceptible to the compounds, while C. albicans was the least susceptible. NC 3 significantly inhibited P. aeruginosa and K. pneumoniae growth at 0.016 mM, while higher concentrations were required for S. aureus, E. faecalis, and C. albicans. NC 5 was most effective against gram-positive bacteria at 0.03 mM. Only NC 4 completely inhibited C. albicans below 1 mM. NC 3, with the lowest concentration for 50% growth inhibition (0.016–0.064 mM), showed promising antibacterial potential against specific AMR-related proteins (bleomycin resistance protein, HTH-type transcriptional regulator QacR, and streptogramin A acetyltransferase), suggesting that this class of compounds could enhance or restore the activity of established antibiotics.
PB  - MDPI
T2  - Pharmaceutics
T1  - Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides
VL  - 16
IS  - 8
DO  - 10.3390/pharmaceutics16081084
ER  - 

@article{
author = "Anić-Marković, Bojana and Marinković, Aleksandar and Antić-Stanković, Jelena and Mijatović, Stefan and Cvijetić, Ilija and Simić, Milena and Aranđelović, Irena",
year = "2024",
abstract = "Antioxidants are promising compounds with antimicrobial activity against drug-resistant pathogens, especially when combined with conventional antimicrobials. Our study aimed to characterize the structure of nicotinamides synthesized from nicotinic acid and thiocarbohydrazones and to evaluate their antibacterial and antifungal activity. Seven nicotinic acid hydrazides (NC 1–7) were synthesized using mono-thiocarbohydrazones with hydroxyl group substituents, along with quinolone, phenolic, and pyridine rings known for their antimicrobial activity. The in vitro antimicrobial activity of NC 1–7, at concentrations ranging from 0.001 to 1 mM, was tested against Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (NCIMB 9111), and Candida albicans (ATCC 24433) using the broth microdilution method per EUCAST 2024 guidelines. Microorganism survival percentages were calculated based on optical density, and target fishing using the PharmMapper database identified potential molecular targets. The results showed that P. aeruginosa was most susceptible to the compounds, while C. albicans was the least susceptible. NC 3 significantly inhibited P. aeruginosa and K. pneumoniae growth at 0.016 mM, while higher concentrations were required for S. aureus, E. faecalis, and C. albicans. NC 5 was most effective against gram-positive bacteria at 0.03 mM. Only NC 4 completely inhibited C. albicans below 1 mM. NC 3, with the lowest concentration for 50% growth inhibition (0.016–0.064 mM), showed promising antibacterial potential against specific AMR-related proteins (bleomycin resistance protein, HTH-type transcriptional regulator QacR, and streptogramin A acetyltransferase), suggesting that this class of compounds could enhance or restore the activity of established antibiotics.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides",
volume = "16",
number = "8",
doi = "10.3390/pharmaceutics16081084"
}

Anić-Marković, B., Marinković, A., Antić-Stanković, J., Mijatović, S., Cvijetić, I., Simić, M.,& Aranđelović, I.. (2024). Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides. in Pharmaceutics
MDPI., 16(8).
https://doi.org/10.3390/pharmaceutics16081084

Anić-Marković B, Marinković A, Antić-Stanković J, Mijatović S, Cvijetić I, Simić M, Aranđelović I. Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides. in Pharmaceutics. 2024;16(8).
doi:10.3390/pharmaceutics16081084 .

Anić-Marković, Bojana, Marinković, Aleksandar, Antić-Stanković, Jelena, Mijatović, Stefan, Cvijetić, Ilija, Simić, Milena, Aranđelović, Irena, "Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides" in Pharmaceutics, 16, no. 8 (2024),
https://doi.org/10.3390/pharmaceutics16081084 . .