TY  - JOUR
AU  - Baralić, Katarina
AU  - Božović, Predrag
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5680
AB  - Even at low levels, exposure to ionising radiation can lead to eye damage. However, the underlying molecular mechanisms are not yet fully
understood. We aimed to address this gap with a comprehensive in silico approach to the issue. For this purpose we relied on the Comparative
Toxicogenomics Database (CTD), ToppGene Suite, Cytoscape, GeneMANIA, and Metascape to identify six key regulator genes associated
with radiation-induced eye damage (ATM, CRYAB, SIRT1, TGFB1, TREX1, and YAP1), all of which have physical interactions. Some
of the identified molecular functions revolve around DNA repair mechanisms, while others are involved in protein binding, enzymatic
activities, metabolic processes, and post-translational protein modifications. The biological processes are mostly centred on response to
DNA damage, the p53 signalling pathway in particular. We identified a significant role of several miRNAs, such as hsa-miR-183 and hsamiR-589, in the mechanisms behind ionising radiation-induced eye injuries. Our study offers a valuable method for gaining deeper insights
into the adverse effects of radiation exposure.
PB  - Institute for Medical Research and Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining
VL  - 75
IS  - 2
SP  - 91
EP  - 101
DO  - 10.2478/aiht-2024-75-3817
ER  - 

@article{
author = "Baralić, Katarina and Božović, Predrag and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Even at low levels, exposure to ionising radiation can lead to eye damage. However, the underlying molecular mechanisms are not yet fully
understood. We aimed to address this gap with a comprehensive in silico approach to the issue. For this purpose we relied on the Comparative
Toxicogenomics Database (CTD), ToppGene Suite, Cytoscape, GeneMANIA, and Metascape to identify six key regulator genes associated
with radiation-induced eye damage (ATM, CRYAB, SIRT1, TGFB1, TREX1, and YAP1), all of which have physical interactions. Some
of the identified molecular functions revolve around DNA repair mechanisms, while others are involved in protein binding, enzymatic
activities, metabolic processes, and post-translational protein modifications. The biological processes are mostly centred on response to
DNA damage, the p53 signalling pathway in particular. We identified a significant role of several miRNAs, such as hsa-miR-183 and hsamiR-589, in the mechanisms behind ionising radiation-induced eye injuries. Our study offers a valuable method for gaining deeper insights
into the adverse effects of radiation exposure.",
publisher = "Institute for Medical Research and Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining",
volume = "75",
number = "2",
pages = "91-101",
doi = "10.2478/aiht-2024-75-3817"
}

Baralić, K., Božović, P.,& Đukić-Ćosić, D.. (2024). Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Institute for Medical Research and Occupational Health, Zagreb., 75(2), 91-101.
https://doi.org/10.2478/aiht-2024-75-3817

Baralić K, Božović P, Đukić-Ćosić D. Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(2):91-101.
doi:10.2478/aiht-2024-75-3817 .

Baralić, Katarina, Božović, Predrag, Đukić-Ćosić, Danijela, "Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 2 (2024):91-101,
https://doi.org/10.2478/aiht-2024-75-3817 . .

TY  - JOUR
AU  - Umićević, Nina
AU  - Kotur-Stevuljević, Jelena
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5592
AB  - This study aimed to assess the redox status and trace metal levels in 49 shoe industry workers (11 men and 38 women) occupationally exposed to a mixture of volatile organic compounds (VOCs), which includes aliphatic hydrocarbons, aromatic hydrocarbons, ketones, esters, ethers, and carboxylic acids. All measured VOCs were below the permitted occupational exposure limits. The control group included 50 unexposed participants (25 men and 25 women). The following plasma parameters were analysed: superoxide anion (O2•-), advanced oxidation protein products (AOPP), total oxidative status (TOS), prooxidant-antioxidant balance (PAB), oxidative stress index (OSI), superoxide dismutase (SOD) and paraoxonase-1 (PON1) enzyme activity, total SH group content (SHG), and total antioxidant status (TAS). Trace metal levels (copper, zinc, iron, magnesium, and manganese) were analysed in whole blood. All oxidative stress and antioxidative defence parameters were higher in the exposed workers than controls, except for PON1 activity. Higher Fe, Mg, and Zn, and lower Cu were observed in the exposed vs control men, while the exposed women had higher Fe and lower Mg, Zn, and Cu than their controls. Our findings confirm that combined exposure to a mixture of VOCs, even at permitted levels, may result in additive or synergistic adverse health effects and related disorders. This raises concern about current risk assessments, which mainly rely on the effects of individual chemicals, and calls for risk assessment approaches that can explain combined exposure to multiple chemicals.
AB  - Cilj istraživanja bio je procijeniti redoks status i koncentracije metala u tragovima kod 49 radnika (11 muškaraca i 38 žena) zaposlenih u
industriji obuće, izloženih mješavini hlapljivih organskih spojeva (engl. volatile organic compounds, krat. VOC) koju su činili alifatski i aromatični
ugljikovodici, ketoni, esteri, eteri i karboksilne kiseline. Sve izmjerene razine VOC bile su ispod dozvoljenih granica profesionalne izloženosti.
U kontrolnoj je skupini bilo 50 neizloženih ispitanika (25 muškaraca i 25 žena). U plazmi obućarskih radnika analizirani su sljedeći parametri:
superoksid anion radikal (O2
•-
), uznapredovali produkti oksidacije proteina (AOPP), totalni oksidacijski status (TOS), prooksidacijsko-
antioksidacijski balans (PAB), indeks oksidacijskoga stresa (OSI), aktivnost superoksid-dismutaze (SOD), aktivnost enzima paraoksonaze-1
(PON1), ukupni sadržaj sulfhidrilnih grupa (SHG) i totalni antioksidacijski status (TAS). Koncentracije metala u tragovima (bakar, cink,
željezo, magnezij i mangan) analizirane su u punoj krvi. U usporedbi s kontrolnom skupinom, svi parametri oksidacijskoga stresa i
antioksidacijske obrane u izloženih radnika bili su povišeni, osim aktivnosti PON1. Povećana koncentracija Fe, Mg i Zn te smanjena
koncentracija Cu uočene su u izloženih muškaraca u usporedbi s kontrolnima, a u žena je primijećena povišena koncentracija Fe, a niža
koncentracija Mg, Zn i Cu u odnosu na kontrolnu skupinu. Naši nalazi potvrđuju da kombinirano izlaganje mješavini VOC, čak i pri
dozvoljenim koncentracijama, može dovesti do aditivnih ili sinergističkih štetnih učinaka na zdravlje i popratnih poremećaja. Trenutna
procjena rizika, koja se uglavnom oslanja na učinke pojedinačnih kemikalija, izaziva zabrinutost i upućuje na potrebu za novim pristupima
koji će objasniti kombiniranu izloženost većem broju kemikalija.
PB  - Sciendo
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds
T1  - Povećani oksidacijski stres u obućarskih radnika izloženih mješavini niskih razina hlapljivih organskih spojeva
VL  - 75
IS  - 1
SP  - 51
EP  - 60
DO  - 10.2478/aiht-2024-75-3804
ER  - 

@article{
author = "Umićević, Nina and Kotur-Stevuljević, Jelena and Baralić, Katarina and Đukić-Ćosić, Danijela and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana",
year = "2024",
abstract = "This study aimed to assess the redox status and trace metal levels in 49 shoe industry workers (11 men and 38 women) occupationally exposed to a mixture of volatile organic compounds (VOCs), which includes aliphatic hydrocarbons, aromatic hydrocarbons, ketones, esters, ethers, and carboxylic acids. All measured VOCs were below the permitted occupational exposure limits. The control group included 50 unexposed participants (25 men and 25 women). The following plasma parameters were analysed: superoxide anion (O2•-), advanced oxidation protein products (AOPP), total oxidative status (TOS), prooxidant-antioxidant balance (PAB), oxidative stress index (OSI), superoxide dismutase (SOD) and paraoxonase-1 (PON1) enzyme activity, total SH group content (SHG), and total antioxidant status (TAS). Trace metal levels (copper, zinc, iron, magnesium, and manganese) were analysed in whole blood. All oxidative stress and antioxidative defence parameters were higher in the exposed workers than controls, except for PON1 activity. Higher Fe, Mg, and Zn, and lower Cu were observed in the exposed vs control men, while the exposed women had higher Fe and lower Mg, Zn, and Cu than their controls. Our findings confirm that combined exposure to a mixture of VOCs, even at permitted levels, may result in additive or synergistic adverse health effects and related disorders. This raises concern about current risk assessments, which mainly rely on the effects of individual chemicals, and calls for risk assessment approaches that can explain combined exposure to multiple chemicals., Cilj istraživanja bio je procijeniti redoks status i koncentracije metala u tragovima kod 49 radnika (11 muškaraca i 38 žena) zaposlenih u
industriji obuće, izloženih mješavini hlapljivih organskih spojeva (engl. volatile organic compounds, krat. VOC) koju su činili alifatski i aromatični
ugljikovodici, ketoni, esteri, eteri i karboksilne kiseline. Sve izmjerene razine VOC bile su ispod dozvoljenih granica profesionalne izloženosti.
U kontrolnoj je skupini bilo 50 neizloženih ispitanika (25 muškaraca i 25 žena). U plazmi obućarskih radnika analizirani su sljedeći parametri:
superoksid anion radikal (O2
•-
), uznapredovali produkti oksidacije proteina (AOPP), totalni oksidacijski status (TOS), prooksidacijsko-
antioksidacijski balans (PAB), indeks oksidacijskoga stresa (OSI), aktivnost superoksid-dismutaze (SOD), aktivnost enzima paraoksonaze-1
(PON1), ukupni sadržaj sulfhidrilnih grupa (SHG) i totalni antioksidacijski status (TAS). Koncentracije metala u tragovima (bakar, cink,
željezo, magnezij i mangan) analizirane su u punoj krvi. U usporedbi s kontrolnom skupinom, svi parametri oksidacijskoga stresa i
antioksidacijske obrane u izloženih radnika bili su povišeni, osim aktivnosti PON1. Povećana koncentracija Fe, Mg i Zn te smanjena
koncentracija Cu uočene su u izloženih muškaraca u usporedbi s kontrolnima, a u žena je primijećena povišena koncentracija Fe, a niža
koncentracija Mg, Zn i Cu u odnosu na kontrolnu skupinu. Naši nalazi potvrđuju da kombinirano izlaganje mješavini VOC, čak i pri
dozvoljenim koncentracijama, može dovesti do aditivnih ili sinergističkih štetnih učinaka na zdravlje i popratnih poremećaja. Trenutna
procjena rizika, koja se uglavnom oslanja na učinke pojedinačnih kemikalija, izaziva zabrinutost i upućuje na potrebu za novim pristupima
koji će objasniti kombiniranu izloženost većem broju kemikalija.",
publisher = "Sciendo",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds, Povećani oksidacijski stres u obućarskih radnika izloženih mješavini niskih razina hlapljivih organskih spojeva",
volume = "75",
number = "1",
pages = "51-60",
doi = "10.2478/aiht-2024-75-3804"
}

Umićević, N., Kotur-Stevuljević, J., Baralić, K., Đukić-Ćosić, D., Antonijević-Miljaković, E., Buha-Đorđević, A., Ćurčić, M., Bulat, Z.,& Antonijević, B.. (2024). Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Sciendo., 75(1), 51-60.
https://doi.org/10.2478/aiht-2024-75-3804

Umićević N, Kotur-Stevuljević J, Baralić K, Đukić-Ćosić D, Antonijević-Miljaković E, Buha-Đorđević A, Ćurčić M, Bulat Z, Antonijević B. Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(1):51-60.
doi:10.2478/aiht-2024-75-3804 .

Umićević, Nina, Kotur-Stevuljević, Jelena, Baralić, Katarina, Đukić-Ćosić, Danijela, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, "Increased oxidative stress in shoe industry workers with low-level exposure to a mixture of volatile organic compounds" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 1 (2024):51-60,
https://doi.org/10.2478/aiht-2024-75-3804 . .

TY  - JOUR
AU  - Živanović, Jovana
AU  - Baralić, Katarina
AU  - Živančević, Katarina
AU  - Božić, Dragica
AU  - Marić, Đurđica
AU  - Antonijević Miljaković, Evica
AU  - Buha Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5681
AB  - COVID-19 can cause a range of complications, including cardiovascular, renal, and/or respiratory insufficiencies, yet little is known of
its potential effects in persons exposed to toxic metals. The aim of this study was to answer this question with in silico toxicogenomic
methods that can provide molecular insights into COVID-19 complications owed to exposure to arsenic, cadmium, lead, mercury, nickel,
and chromium. For this purpose we relied on the Comparative Toxicogenomic Database (CTD), GeneMANIA, and ToppGene Suite
portal and identified a set of five common genes (IL1B, CXCL8, IL6, IL10, TNF) for the six metals and COVID-19, all of which code
for pro-inflammatory and anti-inflammatory cytokines. The list was expanded with additional 20 related genes. Physical interactions are
the most common between the genes affected by the six metals (77.64 %), while the dominant interaction between the genes affected by
each metal separately is co-expression (As 56.35 %, Cd 64.07 %, Pb 71.5 %, Hg 81.91 %, Ni 64.28 %, Cr 88.51 %). Biological processes,
molecular functions, and pathways in which these 25 genes participate are closely related to cytokines and cytokine storm implicated in
the development of COVID-19 complications. In other words, our findings confirm that exposure to toxic metals, alone or in combinations,
might escalate COVID-19 severity.
AB  - COVID-19 može izazvati niz komplikacija, uključujući kardiovaskularnu, bubrežnu i/ili respiratornu insuficijenciju, ali se malo zna o
njegovim potencijalnim učincima u osoba koje su izložene toksičnim metalima. Cilj ovog istraživanja bio je odgovoriti na to pitanje pomoću
in silico toksikogenomske metode, koja može pružiti molekularni uvid u komplikacije bolesti COVID-19 uslijed izloženosti arsenu, kadmiju,
olovu, živi, niklu i kromu. U tu su svrhu korišteni Komparativna toksikogenomska baza podataka (CTD), GeneMANIA i ToppGene Suite
portal te je identificirana skupina od pet zajedničkih gena (IL1B, CXCL8, IL6, IL10, TNF) za šest metala i COVID-19, koji svi kodiraju
proinflamatorne i antiinflamatorne citokine. Lista je proširena s dodatnih 20 srodnih gena. Fizičke interakcije dominirale su između gena
na koje utječe kombinacija ispitivanih metala (77,64 %), a koekspresija je dominantna interakcija između gena na koje djeluju pojedinačni
metali (As 56,35 %, Cd 64,07 %, Pb 71,5 %, Hg 81,91 %, Ni 64,28 %, Cr 88,51 %). Biološki procesi, molekulske funkcije i putovi u kojima
sudjeluje tih 25 gena blisko su povezani s citokinima i citokinskom olujom, koja je uključena u razvoj komplikacija bolesti COVID-19.
Drugim riječima, ovi rezultati potvrđuju da izloženost toksičnim metalima, bilo pojedinačno ili u kombinaciji, može dovesti do razvoja
težih oblika bolesti COVID-19.
PB  - Institute for Medical Research and Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights
T1  - In silico analiza utjecaja toksičnih metala na komplikacije bolesti COVID-19: molekularni uvidi
VL  - 75
IS  - 2
SP  - 102
EP  - 109
DO  - 10.2478/aiht-2024-75-3819
ER  - 

@article{
author = "Živanović, Jovana and Baralić, Katarina and Živančević, Katarina and Božić, Dragica and Marić, Đurđica and Antonijević Miljaković, Evica and Buha Đorđević, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "COVID-19 can cause a range of complications, including cardiovascular, renal, and/or respiratory insufficiencies, yet little is known of
its potential effects in persons exposed to toxic metals. The aim of this study was to answer this question with in silico toxicogenomic
methods that can provide molecular insights into COVID-19 complications owed to exposure to arsenic, cadmium, lead, mercury, nickel,
and chromium. For this purpose we relied on the Comparative Toxicogenomic Database (CTD), GeneMANIA, and ToppGene Suite
portal and identified a set of five common genes (IL1B, CXCL8, IL6, IL10, TNF) for the six metals and COVID-19, all of which code
for pro-inflammatory and anti-inflammatory cytokines. The list was expanded with additional 20 related genes. Physical interactions are
the most common between the genes affected by the six metals (77.64 %), while the dominant interaction between the genes affected by
each metal separately is co-expression (As 56.35 %, Cd 64.07 %, Pb 71.5 %, Hg 81.91 %, Ni 64.28 %, Cr 88.51 %). Biological processes,
molecular functions, and pathways in which these 25 genes participate are closely related to cytokines and cytokine storm implicated in
the development of COVID-19 complications. In other words, our findings confirm that exposure to toxic metals, alone or in combinations,
might escalate COVID-19 severity., COVID-19 može izazvati niz komplikacija, uključujući kardiovaskularnu, bubrežnu i/ili respiratornu insuficijenciju, ali se malo zna o
njegovim potencijalnim učincima u osoba koje su izložene toksičnim metalima. Cilj ovog istraživanja bio je odgovoriti na to pitanje pomoću
in silico toksikogenomske metode, koja može pružiti molekularni uvid u komplikacije bolesti COVID-19 uslijed izloženosti arsenu, kadmiju,
olovu, živi, niklu i kromu. U tu su svrhu korišteni Komparativna toksikogenomska baza podataka (CTD), GeneMANIA i ToppGene Suite
portal te je identificirana skupina od pet zajedničkih gena (IL1B, CXCL8, IL6, IL10, TNF) za šest metala i COVID-19, koji svi kodiraju
proinflamatorne i antiinflamatorne citokine. Lista je proširena s dodatnih 20 srodnih gena. Fizičke interakcije dominirale su između gena
na koje utječe kombinacija ispitivanih metala (77,64 %), a koekspresija je dominantna interakcija između gena na koje djeluju pojedinačni
metali (As 56,35 %, Cd 64,07 %, Pb 71,5 %, Hg 81,91 %, Ni 64,28 %, Cr 88,51 %). Biološki procesi, molekulske funkcije i putovi u kojima
sudjeluje tih 25 gena blisko su povezani s citokinima i citokinskom olujom, koja je uključena u razvoj komplikacija bolesti COVID-19.
Drugim riječima, ovi rezultati potvrđuju da izloženost toksičnim metalima, bilo pojedinačno ili u kombinaciji, može dovesti do razvoja
težih oblika bolesti COVID-19.",
publisher = "Institute for Medical Research and Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights, In silico analiza utjecaja toksičnih metala na komplikacije bolesti COVID-19: molekularni uvidi",
volume = "75",
number = "2",
pages = "102-109",
doi = "10.2478/aiht-2024-75-3819"
}

Živanović, J., Baralić, K., Živančević, K., Božić, D., Marić, Đ., Antonijević Miljaković, E., Buha Đorđević, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Institute for Medical Research and Occupational Health, Zagreb., 75(2), 102-109.
https://doi.org/10.2478/aiht-2024-75-3819

Živanović J, Baralić K, Živančević K, Božić D, Marić Đ, Antonijević Miljaković E, Buha Đorđević A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(2):102-109.
doi:10.2478/aiht-2024-75-3819 .

Živanović, Jovana, Baralić, Katarina, Živančević, Katarina, Božić, Dragica, Marić, Đurđica, Antonijević Miljaković, Evica, Buha Đorđević, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 2 (2024):102-109,
https://doi.org/10.2478/aiht-2024-75-3819 . .

TY  - JOUR
AU  - Mulat Simachew, Yonas
AU  - Mihajlović, Marija
AU  - Antonić, Tamara
AU  - Miloševski Lomić, Gordana
AU  - Peco Antić, Amira
AU  - Jovanović, Dragana
AU  - Paripović, Dušan
AU  - Stefanović, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5679
AB  - Background: The pathophysiological mechanisms crucial in
the development of nephrotic syndrome (NS) in the pediatric population are still not fully understood. This study
aimed to investigate the relationship between hypertension, oxidative stress, and inflammation in pediatric
patients during the acute phase of the disease.
Methods: The study included 33 children, aged 2 to 9
years, with nephrotic syndrome. Blood samples were collected during the acute phase and remission. Parameters of
oxidative status were determined, including total oxidative
status (TOS), advanced oxidation protein products (AOPP),
prooxidant-antioxidant balance (PAB), sulfhydryl groups (-
SH), paraoxonase 1 (PON1), and total antioxidant status
(TAS) in serum, measured spectrophotometrically. Inflam-
matory parameters such as pentraxin 3 (PTX3), leptin,
programmed cell death ligand 1 (PD-L1), and E-cadherin
were determined using enzyme-linked immunosorbent
assay (ELISA).
Results: Patients with nephrotic syndrome and hypertension had significantly higher levels of advanced oxidation
protein products and total antioxidant status (p=0.029 and
p=0.003, respectively). During the acute phase of the dis-
ease, lower activity of sulfhydryl groups and paraoxonase 1
was observed compared to remission (p<0.001, for both).
AB  - Uvod: Patofiziološki mehanizmi ključni u razvoju nefrotskog sindroma (NS) u pedijatrijskoj populaciji još uvek nisu u potpunosti razjašnjeni. Ova studija ima za cilj proučavanje sinergističkog delovanja oksidativnog stresa i inflamacije u patogenezi NS. Takođe, jedan od ciljeva ove studije je i ispitivanje veze hipertenzije sa stepenom oksidativnog stresa i inflama - cije kod pacijenata u akutnoj fazi bolesti. Metode: U studiju je uključeno 33 dece sa NS uzrasta od 2 do 9 godina. Uzorci krvi su prikupljeni tokom akutne faze i remisije. Od parametara oksidativnog statusa određivani su: totalni oksidativni status (TOS), uznapredovali proizvodi oksidacije proteina (AOPP), balans prooksidans-antioksidans (PAB), sulfhidrilne grupe (-SH), paraoksonaza 1 (PON1) i ukupan antioksidativni status (TAS) u serumu su mereni spektrofometrijski, a od parametara inflamacije su pentraksin 3 (PTX3), leptin, ligand programirane smrti ćelije 1 (PD-L1) i E-kadherin određivani metodom enzimskog imunosorbentnog testa (ELISA). Rezultati: Pacijenti sa NS i hipertenzijom imali su značajno više nivoe AOPP i TOS (p=0.029 i p=0.003, respektivno). U akutnoj fazi bolesti su uočene nižu aktivnost -SH i PON1 u poređenju sa remisijom (p<0.001, za oba). Nivoi PTX 3 su bili viši, dok su nivoi leptina bili niži tokom akutne faze (p<0.001, za oba). PTX 3 je korelirao sa AOPP i TAS u akutnoj fazi, ali ne i u remisiji (rs=0.42, p=0.027 i rs=0.43, p=0.025,respektivno). U akutnooj fazi utvrđena je negativna korelacija između AOPP i leptina, koja je nestala u remisiji (rs=-0.42, p=0.028). Zaključak: Rezultati ove studije ukazuju da hipertenzija utiče na markere oksidativnog stresa, a smanjeni antioksidativni kapacitet može doprineti razvoju NS. PTX3 se pojavljuje kao potencijalni marker aktivnosti bolesti, što ukazuje na dinamičku vezu između inflamacije i oksidativnog stresa. Leptin može igrati ulogu u oksidativnom stresu u NS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd
T2  - Journal of Medical Biochemistry
T1  - Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation
T1  - Pedijatrijski nefrotski sindrom: međusobna interakcija oksidativnog stresa i inflamacije
VL  - 43
IS  - 3
SP  - 424
EP  - 435
DO  - 10.5937/jomb0-46526
ER  - 

@article{
author = "Mulat Simachew, Yonas and Mihajlović, Marija and Antonić, Tamara and Miloševski Lomić, Gordana and Peco Antić, Amira and Jovanović, Dragana and Paripović, Dušan and Stefanović, Aleksandra",
year = "2024",
abstract = "Background: The pathophysiological mechanisms crucial in
the development of nephrotic syndrome (NS) in the pediatric population are still not fully understood. This study
aimed to investigate the relationship between hypertension, oxidative stress, and inflammation in pediatric
patients during the acute phase of the disease.
Methods: The study included 33 children, aged 2 to 9
years, with nephrotic syndrome. Blood samples were collected during the acute phase and remission. Parameters of
oxidative status were determined, including total oxidative
status (TOS), advanced oxidation protein products (AOPP),
prooxidant-antioxidant balance (PAB), sulfhydryl groups (-
SH), paraoxonase 1 (PON1), and total antioxidant status
(TAS) in serum, measured spectrophotometrically. Inflam-
matory parameters such as pentraxin 3 (PTX3), leptin,
programmed cell death ligand 1 (PD-L1), and E-cadherin
were determined using enzyme-linked immunosorbent
assay (ELISA).
Results: Patients with nephrotic syndrome and hypertension had significantly higher levels of advanced oxidation
protein products and total antioxidant status (p=0.029 and
p=0.003, respectively). During the acute phase of the dis-
ease, lower activity of sulfhydryl groups and paraoxonase 1
was observed compared to remission (p<0.001, for both)., Uvod: Patofiziološki mehanizmi ključni u razvoju nefrotskog sindroma (NS) u pedijatrijskoj populaciji još uvek nisu u potpunosti razjašnjeni. Ova studija ima za cilj proučavanje sinergističkog delovanja oksidativnog stresa i inflamacije u patogenezi NS. Takođe, jedan od ciljeva ove studije je i ispitivanje veze hipertenzije sa stepenom oksidativnog stresa i inflama - cije kod pacijenata u akutnoj fazi bolesti. Metode: U studiju je uključeno 33 dece sa NS uzrasta od 2 do 9 godina. Uzorci krvi su prikupljeni tokom akutne faze i remisije. Od parametara oksidativnog statusa određivani su: totalni oksidativni status (TOS), uznapredovali proizvodi oksidacije proteina (AOPP), balans prooksidans-antioksidans (PAB), sulfhidrilne grupe (-SH), paraoksonaza 1 (PON1) i ukupan antioksidativni status (TAS) u serumu su mereni spektrofometrijski, a od parametara inflamacije su pentraksin 3 (PTX3), leptin, ligand programirane smrti ćelije 1 (PD-L1) i E-kadherin određivani metodom enzimskog imunosorbentnog testa (ELISA). Rezultati: Pacijenti sa NS i hipertenzijom imali su značajno više nivoe AOPP i TOS (p=0.029 i p=0.003, respektivno). U akutnoj fazi bolesti su uočene nižu aktivnost -SH i PON1 u poređenju sa remisijom (p<0.001, za oba). Nivoi PTX 3 su bili viši, dok su nivoi leptina bili niži tokom akutne faze (p<0.001, za oba). PTX 3 je korelirao sa AOPP i TAS u akutnoj fazi, ali ne i u remisiji (rs=0.42, p=0.027 i rs=0.43, p=0.025,respektivno). U akutnooj fazi utvrđena je negativna korelacija između AOPP i leptina, koja je nestala u remisiji (rs=-0.42, p=0.028). Zaključak: Rezultati ove studije ukazuju da hipertenzija utiče na markere oksidativnog stresa, a smanjeni antioksidativni kapacitet može doprineti razvoju NS. PTX3 se pojavljuje kao potencijalni marker aktivnosti bolesti, što ukazuje na dinamičku vezu između inflamacije i oksidativnog stresa. Leptin može igrati ulogu u oksidativnom stresu u NS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd",
journal = "Journal of Medical Biochemistry",
title = "Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation, Pedijatrijski nefrotski sindrom: međusobna interakcija oksidativnog stresa i inflamacije",
volume = "43",
number = "3",
pages = "424-435",
doi = "10.5937/jomb0-46526"
}

Mulat Simachew, Y., Mihajlović, M., Antonić, T., Miloševski Lomić, G., Peco Antić, A., Jovanović, D., Paripović, D.,& Stefanović, A.. (2024). Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd., 43(3), 424-435.
https://doi.org/10.5937/jomb0-46526

Mulat Simachew Y, Mihajlović M, Antonić T, Miloševski Lomić G, Peco Antić A, Jovanović D, Paripović D, Stefanović A. Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation. in Journal of Medical Biochemistry. 2024;43(3):424-435.
doi:10.5937/jomb0-46526 .

Mulat Simachew, Yonas, Mihajlović, Marija, Antonić, Tamara, Miloševski Lomić, Gordana, Peco Antić, Amira, Jovanović, Dragana, Paripović, Dušan, Stefanović, Aleksandra, "Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation" in Journal of Medical Biochemistry, 43, no. 3 (2024):424-435,
https://doi.org/10.5937/jomb0-46526 . .

TY  - JOUR
AU  - Milinčić, Danijel
AU  - Vidović, Bojana
AU  - Gašić, Uroš
AU  - Milenković, Milan
AU  - Kostić, Aleksandar
AU  - Stanojević, Slađana
AU  - Ilić, Tijana
AU  - Pešić, Mirjana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5677
AB  - The aim of this study was to identify and characterise different classes of bioactive compounds from freeze-dried red goji berries (RGB) grown in Serbia, using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC Q-ToF MS). In addition, this study aims to demonstrate the importance of applying the advanced UHPLC Q-ToF MS technique in the identification of various biocompounds. The analysis showed the presence of 28 phenolic compounds, 3 organic acids, and 26 phenylamides. The 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG) was identified by UHPLC Q-ToF MS and quantified by standardised UHPLC-DAD method. Most of the compounds detected were derivatives of caffeic acid and ferulic acid, followed by quercetin derivatives. Among the phenylamides, several glucosylated caffeoyl and/or dihydrocaffeoyl derivatives of spermidine and spermine were characterized, confirming their recent characterization. Some glycosylated/non-glycosylated putrescine derivatives and caffeoyl-dihydrocaffeoyl-feruloyl spermidines were identified in goji berriesfor the first time. Their tentative structures and fragmentations were proposed.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides
VL  - 456
DO  - 10.1016/j.foodchem.2024.140044
ER  - 

@article{
author = "Milinčić, Danijel and Vidović, Bojana and Gašić, Uroš and Milenković, Milan and Kostić, Aleksandar and Stanojević, Slađana and Ilić, Tijana and Pešić, Mirjana",
year = "2024",
abstract = "The aim of this study was to identify and characterise different classes of bioactive compounds from freeze-dried red goji berries (RGB) grown in Serbia, using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC Q-ToF MS). In addition, this study aims to demonstrate the importance of applying the advanced UHPLC Q-ToF MS technique in the identification of various biocompounds. The analysis showed the presence of 28 phenolic compounds, 3 organic acids, and 26 phenylamides. The 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG) was identified by UHPLC Q-ToF MS and quantified by standardised UHPLC-DAD method. Most of the compounds detected were derivatives of caffeic acid and ferulic acid, followed by quercetin derivatives. Among the phenylamides, several glucosylated caffeoyl and/or dihydrocaffeoyl derivatives of spermidine and spermine were characterized, confirming their recent characterization. Some glycosylated/non-glycosylated putrescine derivatives and caffeoyl-dihydrocaffeoyl-feruloyl spermidines were identified in goji berriesfor the first time. Their tentative structures and fragmentations were proposed.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides",
volume = "456",
doi = "10.1016/j.foodchem.2024.140044"
}

Milinčić, D., Vidović, B., Gašić, U., Milenković, M., Kostić, A., Stanojević, S., Ilić, T.,& Pešić, M.. (2024). A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides. in Food Chemistry
Elsevier Ltd., 456.
https://doi.org/10.1016/j.foodchem.2024.140044

Milinčić D, Vidović B, Gašić U, Milenković M, Kostić A, Stanojević S, Ilić T, Pešić M. A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides. in Food Chemistry. 2024;456.
doi:10.1016/j.foodchem.2024.140044 .

Milinčić, Danijel, Vidović, Bojana, Gašić, Uroš, Milenković, Milan, Kostić, Aleksandar, Stanojević, Slađana, Ilić, Tijana, Pešić, Mirjana, "A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides" in Food Chemistry, 456 (2024),
https://doi.org/10.1016/j.foodchem.2024.140044 . .

TY  - JOUR
AU  - Senćanski, Dejan
AU  - Tadić, Ivana
AU  - Jocić, Dragana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5678
AB  - Background: Emotional intelligence (EI) is a critical set of skills that impacts clinical pharmacists’ well-being and positively influences high-level patient-centred care. Describing pharmacists’ perceptions may support the integration of EI development approaches into their professional development continuum. Aim: The aim of this study was to analyse pharmacists’ perceptions of the characteristics of emotionally intelligent clinical pharmacists, the importance of EI in clinical practice, and educational models and approaches to enhancing EI. Method: A qualitative study with a focus group methodology was conducted with pharmacy practitioners using a semi-structured guide grounded in the EI competency framework and existing qualitative research methodology practices. Purposive sampling was conducted until information and meaning saturation occurred. The focus group recordings were transcribed and independently coded by two researchers. The conventional content analysis of qualitative data was applied with the inductive thematic approach at its core. Results: According to the 17 focus group participants, emotionally intelligent clinical pharmacists are perceived as self-confident communicators who control and manage emotions, work well under pressure, and handle every situation effectively. Emotional self-control, self-awareness, awareness of others, tolerance, understanding, and empathy have emerged as key EI competencies required for challenges in clinical practice. EI lectures with reflections from clinical applications, behaviour modelling, and behaviour-changing methods were perceived to be of particular importance for pharmacist education and development programmes. Conclusion: Postgraduate pharmacy practitioners perceived EI competencies as necessary for their professional success and high-quality patient-centred care. They suggested that EI competencies be a focal point in pharmacy professional development programmes.
PB  - Springer Nature
T2  - International Journal of Clinical Pharmacy
T1  - Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists
DO  - 10.1007/s11096-024-01748-4
ER  - 

@article{
author = "Senćanski, Dejan and Tadić, Ivana and Jocić, Dragana and Marinković, Valentina",
year = "2024",
abstract = "Background: Emotional intelligence (EI) is a critical set of skills that impacts clinical pharmacists’ well-being and positively influences high-level patient-centred care. Describing pharmacists’ perceptions may support the integration of EI development approaches into their professional development continuum. Aim: The aim of this study was to analyse pharmacists’ perceptions of the characteristics of emotionally intelligent clinical pharmacists, the importance of EI in clinical practice, and educational models and approaches to enhancing EI. Method: A qualitative study with a focus group methodology was conducted with pharmacy practitioners using a semi-structured guide grounded in the EI competency framework and existing qualitative research methodology practices. Purposive sampling was conducted until information and meaning saturation occurred. The focus group recordings were transcribed and independently coded by two researchers. The conventional content analysis of qualitative data was applied with the inductive thematic approach at its core. Results: According to the 17 focus group participants, emotionally intelligent clinical pharmacists are perceived as self-confident communicators who control and manage emotions, work well under pressure, and handle every situation effectively. Emotional self-control, self-awareness, awareness of others, tolerance, understanding, and empathy have emerged as key EI competencies required for challenges in clinical practice. EI lectures with reflections from clinical applications, behaviour modelling, and behaviour-changing methods were perceived to be of particular importance for pharmacist education and development programmes. Conclusion: Postgraduate pharmacy practitioners perceived EI competencies as necessary for their professional success and high-quality patient-centred care. They suggested that EI competencies be a focal point in pharmacy professional development programmes.",
publisher = "Springer Nature",
journal = "International Journal of Clinical Pharmacy",
title = "Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists",
doi = "10.1007/s11096-024-01748-4"
}

Senćanski, D., Tadić, I., Jocić, D.,& Marinković, V.. (2024). Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists. in International Journal of Clinical Pharmacy
Springer Nature..
https://doi.org/10.1007/s11096-024-01748-4

Senćanski D, Tadić I, Jocić D, Marinković V. Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists. in International Journal of Clinical Pharmacy. 2024;.
doi:10.1007/s11096-024-01748-4 .

Senćanski, Dejan, Tadić, Ivana, Jocić, Dragana, Marinković, Valentina, "Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists" in International Journal of Clinical Pharmacy (2024),
https://doi.org/10.1007/s11096-024-01748-4 . .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Demisli, Sortiria
AU  - Savić, Sanela M.
AU  - Savić, Saša R.
AU  - Randjelović, Danijela V.
AU  - Marković, Bojan
AU  - Pantelić, Ivana
AU  - Mitrović, Jelena
AU  - Stanković, Tijana
AU  - Papadimitrou, Vassiliki
AU  - Xenakis, Aristotelis
AU  - Savić, Snežana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5651
AB  - Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties
VL  - 404
DO  - 10.1016/j.molliq.2024.124888
ER  - 

@article{
author = "Đoković, Jelena and Demisli, Sortiria and Savić, Sanela M. and Savić, Saša R. and Randjelović, Danijela V. and Marković, Bojan and Pantelić, Ivana and Mitrović, Jelena and Stanković, Tijana and Papadimitrou, Vassiliki and Xenakis, Aristotelis and Savić, Snežana",
year = "2024",
abstract = "Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties",
volume = "404",
doi = "10.1016/j.molliq.2024.124888"
}

Đoković, J., Demisli, S., Savić, S. M., Savić, S. R., Randjelović, D. V., Marković, B., Pantelić, I., Mitrović, J., Stanković, T., Papadimitrou, V., Xenakis, A.,& Savić, S.. (2024). Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids
Elsevier B.V.., 404.
https://doi.org/10.1016/j.molliq.2024.124888

Đoković J, Demisli S, Savić SM, Savić SR, Randjelović DV, Marković B, Pantelić I, Mitrović J, Stanković T, Papadimitrou V, Xenakis A, Savić S. Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids. 2024;404.
doi:10.1016/j.molliq.2024.124888 .

Đoković, Jelena, Demisli, Sortiria, Savić, Sanela M., Savić, Saša R., Randjelović, Danijela V., Marković, Bojan, Pantelić, Ivana, Mitrović, Jelena, Stanković, Tijana, Papadimitrou, Vassiliki, Xenakis, Aristotelis, Savić, Snežana, "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties" in Journal of Molecular Liquids, 404 (2024),
https://doi.org/10.1016/j.molliq.2024.124888 . .

TY  - CONF
AU  - Vasiljević, Ivana
AU  - Turković, Erna
AU  - Parojčić, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5675
AB  - Selective laser sintering (SLS) represents a 3D printing technology recently introduced as the drug preparation method (1). It was implemented in the fabrication of different dosage forms, including multiparticulate unit (MPU) systems (2–3).
Up to date, maximal drug content incorporated in the SLS printed dosage forms was 92%, in the case of metronidazole-loaded SLS printed tablets (4). However, in the case of MPUs, the drug load was up to 10% (2–3). Due to the difficulties of incorporating high drug loads into 3D printed dosage forms, the aim of this research was to investigate the possibility of printing multiparticulate unit systems containing higher drug loading and evaluate their characteristics (model drug content, particle size and size distribution, flowability, and drug dissolution).
PB  - International Association for Pharmaceutical Technology (APV), Mainz, Germany
PB  - International Society of Drug Delivery Sciences and Technology (APGI), Lille, France
PB  - Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy
C3  - 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
T1  - How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5675
ER  - 

@conference{
author = "Vasiljević, Ivana and Turković, Erna and Parojčić, Jelena",
year = "2024",
abstract = "Selective laser sintering (SLS) represents a 3D printing technology recently introduced as the drug preparation method (1). It was implemented in the fabrication of different dosage forms, including multiparticulate unit (MPU) systems (2–3).
Up to date, maximal drug content incorporated in the SLS printed dosage forms was 92%, in the case of metronidazole-loaded SLS printed tablets (4). However, in the case of MPUs, the drug load was up to 10% (2–3). Due to the difficulties of incorporating high drug loads into 3D printed dosage forms, the aim of this research was to investigate the possibility of printing multiparticulate unit systems containing higher drug loading and evaluate their characteristics (model drug content, particle size and size distribution, flowability, and drug dissolution).",
publisher = "International Association for Pharmaceutical Technology (APV), Mainz, Germany, International Society of Drug Delivery Sciences and Technology (APGI), Lille, France, Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy",
journal = "14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria",
title = "How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5675"
}

Vasiljević, I., Turković, E.,& Parojčić, J.. (2024). How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
International Association for Pharmaceutical Technology (APV), Mainz, Germany..
https://hdl.handle.net/21.15107/rcub_farfar_5675

Vasiljević I, Turković E, Parojčić J. How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria. 2024;.
https://hdl.handle.net/21.15107/rcub_farfar_5675 .

Vasiljević, Ivana, Turković, Erna, Parojčić, Jelena, "How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?" in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria (2024),
https://hdl.handle.net/21.15107/rcub_farfar_5675 .

TY  - CONF
AU  - Stanković, Milan
AU  - Đurđević, Mladen
AU  - Ražić, Slavica
AU  - Đogo-Mračević, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5673
AB  - Za zemljišta urbanih gradskih područja se očekuje da budu zagađena potencijalno toksičnim elementima (PTE), zbog saobraćaja, urbanizacije i industralizacij. Budući da deca provode dosta vremena na igralištima, igrajući se zemljom, njihova izloženost ovim elementima i potencijalni štetni efekti na zdravlje su poslednjih godina postala vrlo značajna tema. [1-2] Cilj ovog rada je bio određivanje sadržaja arsena (As), kadmijuma (Cd), olova (Pb), nikla (Ni), hroma (Cr), bakra (Cu) i cinka (Zn) u 17 uzoraka zemljišta sa različitih dečjih igrališta u urbanoj zoni Beograda. Uzorci su pripremani kiselom mikrotalasnom digestijom, a sadržaj elemenata je određivan metodom induktivno spregnute plazme optičke emisione spektroskopije (ISP-OES). Tačnost metode je procenjena upotrebom sertifikovanog referentnog materijala NIST 2711a, i dobijene su vrednosti  u opsegu  80-120%. Prosečna koncentracija ispitivanih PTE sledi navedeni niz: Zn (68,24 mg/kg) > Ni (60,99 mg/kg) > Cr (51,685 mg/kg)  > Pb (21,615 mg/kg) > Cu (19,785 mg/kg) > As (8,1125 mg/kg) > Cd (0,1566 mg/kg). Koncentracije svih ispitivanih PTE su u saglasnosti sa  legislativma Republike Srbije, Evropske unije (EU) i Svetske zdravstvene organizacije (SZO). S obzirom na potencijal PTE za bioakumulaciju i uključivanje u lanac ishran, vrlo je važno njihovo konstantno praćenje, a u tom smislu ovaj rad može imati doprinos u očuvanju urbane sredine.
AB  - Soils in the urban area are suspected to be contaminated with potentially toxic elements (PTEs), due to traffic, urbanisation, and industrialization. The possibility of adverse health effects and the exposure of children who spend a lot of time in playgrounds and playing with soil, has attracted  a lot of attention in recent years, especially with regard to the potential risk to children's health. [1-2] The aim of this study was to determine the levels of arsenic (As), cadmium (Cd), lead (Pb), nickel (Ni), chromium (Cr), copper (Cu), and zinc (Zn) in 17 soil samples from different children's playgrounds in the city of Belgrade. The samples were prepared by acid assisted microwave digestion and the concentrations of  elements were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Accuracy was assessed using the certified reference material NIST 2711a, and recoveries obtained were in the range of 80-120%. The average concentration of PTEs followed the following order: Zn (68.24 mg/kg) > Ni (60.99 mg/kg) > Cr (51.685 mg/kg)  > Pb (21.615 mg/kg) > Cu (19.785 mg/kg) > As (8.1125 mg/kg) > Cd (0.1566 mg/kg). The average concentration of all metals in soil did not exceed the permissible limits according to the guidelines of Serbian legislation, the European Union (EU), and the World Health Organization (WHO).  Since these PTEs can bioaccumulate and biomagnify in the food chain, their concentration in soil should be continuously monitored so this study should contribute to rising awareness for the protection of the urban environment.

[1] M. Guney, G. J. Zagury, N. Dogan, T. T. Onay. Exposure assessment and risk characterization from trace elements following soil ingestion by children exposed to playgrounds, parks and picnic areas, J Hazard Mater 2010;182(1-3):656-64. 
[2] E. Schachter, A. Gailey, S. Perl Egendorf, H. W. Mielke, Mechanisms of children's soil exposure, Current Problems in Pediatric and Adolescent Health Care 2020, 50(1), 100742.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija
T1  - Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda
T1  - Toxic Elements in Soil of Children's Playgrounds in the Belgrade Urban Area
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5673
ER  - 

@conference{
author = "Stanković, Milan and Đurđević, Mladen and Ražić, Slavica and Đogo-Mračević, Svetlana",
year = "2024",
abstract = "Za zemljišta urbanih gradskih područja se očekuje da budu zagađena potencijalno toksičnim elementima (PTE), zbog saobraćaja, urbanizacije i industralizacij. Budući da deca provode dosta vremena na igralištima, igrajući se zemljom, njihova izloženost ovim elementima i potencijalni štetni efekti na zdravlje su poslednjih godina postala vrlo značajna tema. [1-2] Cilj ovog rada je bio određivanje sadržaja arsena (As), kadmijuma (Cd), olova (Pb), nikla (Ni), hroma (Cr), bakra (Cu) i cinka (Zn) u 17 uzoraka zemljišta sa različitih dečjih igrališta u urbanoj zoni Beograda. Uzorci su pripremani kiselom mikrotalasnom digestijom, a sadržaj elemenata je određivan metodom induktivno spregnute plazme optičke emisione spektroskopije (ISP-OES). Tačnost metode je procenjena upotrebom sertifikovanog referentnog materijala NIST 2711a, i dobijene su vrednosti  u opsegu  80-120%. Prosečna koncentracija ispitivanih PTE sledi navedeni niz: Zn (68,24 mg/kg) > Ni (60,99 mg/kg) > Cr (51,685 mg/kg)  > Pb (21,615 mg/kg) > Cu (19,785 mg/kg) > As (8,1125 mg/kg) > Cd (0,1566 mg/kg). Koncentracije svih ispitivanih PTE su u saglasnosti sa  legislativma Republike Srbije, Evropske unije (EU) i Svetske zdravstvene organizacije (SZO). S obzirom na potencijal PTE za bioakumulaciju i uključivanje u lanac ishran, vrlo je važno njihovo konstantno praćenje, a u tom smislu ovaj rad može imati doprinos u očuvanju urbane sredine., Soils in the urban area are suspected to be contaminated with potentially toxic elements (PTEs), due to traffic, urbanisation, and industrialization. The possibility of adverse health effects and the exposure of children who spend a lot of time in playgrounds and playing with soil, has attracted  a lot of attention in recent years, especially with regard to the potential risk to children's health. [1-2] The aim of this study was to determine the levels of arsenic (As), cadmium (Cd), lead (Pb), nickel (Ni), chromium (Cr), copper (Cu), and zinc (Zn) in 17 soil samples from different children's playgrounds in the city of Belgrade. The samples were prepared by acid assisted microwave digestion and the concentrations of  elements were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Accuracy was assessed using the certified reference material NIST 2711a, and recoveries obtained were in the range of 80-120%. The average concentration of PTEs followed the following order: Zn (68.24 mg/kg) > Ni (60.99 mg/kg) > Cr (51.685 mg/kg)  > Pb (21.615 mg/kg) > Cu (19.785 mg/kg) > As (8.1125 mg/kg) > Cd (0.1566 mg/kg). The average concentration of all metals in soil did not exceed the permissible limits according to the guidelines of Serbian legislation, the European Union (EU), and the World Health Organization (WHO).  Since these PTEs can bioaccumulate and biomagnify in the food chain, their concentration in soil should be continuously monitored so this study should contribute to rising awareness for the protection of the urban environment.

[1] M. Guney, G. J. Zagury, N. Dogan, T. T. Onay. Exposure assessment and risk characterization from trace elements following soil ingestion by children exposed to playgrounds, parks and picnic areas, J Hazard Mater 2010;182(1-3):656-64. 
[2] E. Schachter, A. Gailey, S. Perl Egendorf, H. W. Mielke, Mechanisms of children's soil exposure, Current Problems in Pediatric and Adolescent Health Care 2020, 50(1), 100742.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija",
title = "Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda, Toxic Elements in Soil of Children's Playgrounds in the Belgrade Urban Area",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5673"
}

Stanković, M., Đurđević, M., Ražić, S.,& Đogo-Mračević, S.. (2024). Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda. in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija
Beograd : Srpsko hemijsko društvo., 81-81.
https://hdl.handle.net/21.15107/rcub_farfar_5673

Stanković M, Đurđević M, Ražić S, Đogo-Mračević S. Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda. in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija. 2024;:81-81.
https://hdl.handle.net/21.15107/rcub_farfar_5673 .

Stanković, Milan, Đurđević, Mladen, Ražić, Slavica, Đogo-Mračević, Svetlana, "Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda" in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija (2024):81-81,
https://hdl.handle.net/21.15107/rcub_farfar_5673 .

TY  - THES
AU  - Gajić Bojić, Milica
PY  - 2024
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9640
UR  - https://uvidok.rcub.bg.ac.rs/doccall/bitstream/handle/123456789/5575/Referat.pdf
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:33769/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/138481673
UR  - https://nardus.mpn.gov.rs/handle/123456789/22506
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5672
AB  - U posljednje vrijeme uloga GABA (eng. gamma-aminobutyric acid, GABA) i GABAA receptora (GABAAR) na periferiji postaje sve važnija. Iako su dokazi još uvijek ograničeni, sugerisano je da se hipotenzivni efekti benzodiazepina (BZD) i drugih liganada GABAAR, pored poznatih mehanizama neuromodulacije u centralnom i perifernom nervnom sistemu, ostvaruju i modulacijom "vaskularnih" GABAAR u perifernim krvnim sudovima. Pretpostavili smo da različiti pozitivni alosterni modulatori (PAM) GABAAR ispoljavaju direktne vazodilatatorne efekte preko vaskularnih GABAAR koji su eksprimirani na aorti pacova.Ispitivanja predstavljena u ovoj disertaciji sprovedena su u dva pravca. U jednom pravcu vršeno je dokazivanje prisustva α1-6 i γ2 podjedinica GABAAR u aorti pacova, primjenom RT-PCR (eng. real–time–polymerase chain reaction) i imunohistohemijske analize. U drugom pravcu vršeno je izometrijsko mjerenje kontrakcije izolovanih prstenova pacovske aorte u prisustvu različitih selektivnih i neselektivnih PAM-ova GABAAR: zolpidema (α1–selektivan), XHe–III–074 (α4–selektivan), MP–III–022, MP-III-058, GL-II-74, GL-II-73 (α5–selektivni), DK-I-56-1 (α6–selektivan), SH-I-048A, midazolama i diazepama (neselektivni). Rezultati RT-PCR analize dokazali su ekspresiju iRNK α1–5 podjedinica u homogenatu tkiva torakalne aorte pacova, dok za α6 podjedinicu nije utvrđena ekspresija iRNK. Imunohistohemijskim bojenjem histoloških presjeka pacovske aorte potvrđena je ekspresija proteina α1-5 podjedinice, kao i ekspresija proteina γ2 podjedinice, te je utvrđeno da su identifikovani proteini lokalizovani na vaskularnom glatko-mišićnom sloju aorte pacova. Testovi u kupatilu za rad sa izolovanim prstenovima aorte otkrili su značajne vazodilatacijske efekte svih ispitivanih PAM-ova (preko 50% ostvarene relaksacije prekontrahovanih preparata), pri čemu je diazepam bio najefikasniji ligand, dok je zolpidem pokazao najslabije vaskularne efekte. Flumazenil, kao antagonista BZD mjesta na GABAAR, pokazao je slabu vazoaktivnost per se, ali je značajno smanjio vazodilatacijske efekte testiranih PAM-ova, što upućuje da se njihova vazoaktivnost ostvaruje modulacijom BZD veznog mjesta na vaskularnim GABAAR. Primjenjen u koncentraciji od 10-4 M, flumazenil je značajno smanjio relaksaciju izazvanu midazolamom (P < 0,01), kao i relaksaciju izazvanu MP-III-058 (P < 0,001), i uzrokovao tako pomijeranje njihovih krivih odnosa koncentracija-odgovor udesno i naniže. Vazodilatacijski efekti diazepama nisu smanjeni u prisustvu antagonista (bikukulina, odnosno PK11195), osim onih ostvarenih pri nižim koncentracijama diazepama (10-7 M i 3x10-7 M). TSPO antagonista PK11195 ispoljio je vazodilatacijske efekte na fenilefrin (FE)- prekontrahovanim preparatima, uporedive sa efektima diazepama, što nije bio slučaj sa antagonistima bikukulinom i flumazenilom. Maksimalna efikasnost testiranih PAM-ova postignuta je pri koncentraciji (10-4 M) koju je teško postići u in vivo sistemu, osim u uslovima predoziranja i zloupotrebe lijekova. Primjenjeni u ovako visokoj koncentraciji, vazodilatacijski efekti testiranih PAM-ova bili su uporedivi sa efektima prazosina, kao referentnog antagoniste adrenergičke kontrakciije. Međutim, njihov relaksacijski potencijal značajno je niži od prazosinskog, na šta ukazuju uočene razlike u vrijednostima pEC50. Za sve ispitivane PAM-ove vazodilatacijski efekti bili su izraženiji kod FE- prekontrahovanih u odnosu na KCl- prekontrahovane preparate...
AB  - Recently, the role of GABA (gamma-aminobutyric acid) and GABAA receptors (GABAAR) in the periphery has become increasingly important. Although the evidence is still limited, it has been suggested that the hypotensive effects of benzodiazepines (BZD) and other GABAAR ligands, in addition to the known mechanisms of neuromodulation in the central and peripheral nervous system, are also achieved by the modulation of "vascular" GABAAR in peripheral blood vessels. We hypothesized that various positive allosteric modulators (PAM) of GABAAR exert direct vasodilator effects via vascular GABAAR expressed on rat aorta.The investigations presented in this dissertation were carried out in two directions. In one direction, the presence of α and γ2 subunits of the GABAAR in the rat aorta was demonstrated, using RT-PCR (real–time–polymerase chain reaction) and immunohistochemical analysis. In the second direction, isomeric measurement of contraction of isolated rat aortic rings in the presence of various selective and non-selective PAMs of GABAAR was performed: zolpidem (α1–selective), XHe-III-074 (α4–selective), MP-III-022, MP-III-058, GL-II-74, GL-II-73 (α5–selective), DK-I-56-1 (α6–selective), SH-I-048A, midazolam and diazepam (non-selective).The results of RT-PCR analysis demonstrated the expression of mRNA α1–5 subunits in the tissue homogenate of the rat thoracic aorta, while no mRNA expression was found for the α6 subunit. Immunohistochemical staining of histological sections of the rat aorta confirmed the expression of the α1-5 subunit protein, as well as the expression of the γ2 subunit protein, and it was determined that the identified proteins are localized on the vascular smooth-muscle layer of the rat aorta. Assays with isolated aortic rings revealed significant vasodilatory effects of all tested PAMs (over 50% relaxation achieved in precontracted preparation), where diazepam was the most effective ligand, while zolpidem showed the weakest vascular effects. Flumazenil exhibited weak vasoactivity per se, but significantly prevented the relaxant effects of tested PAMs. Applied at a concentration of 10-4 M, flumazenil significantly reduced midazolam-induced relaxation (P < 0.01), as well as MP-III-058-induced relaxation (P < 0.001), shifting their concentration-response curves to the right and down. The vasodilating effects of diazepam were not reduced in the presence of the antagonist (bicuculline, ie PK11195), except for those achieved at lower concentrations of diazepam (10-7 M and 3x10-7 M). The TSPO antagonist PK11195 exhibited vasodilatory effects on phenylephrine (FE)-precontracted preparations, comparable to the effects of diazepam, which was not the case with the antagonists bicuculline and flumazenil. The maximum efficiency of the tested PAMs was achieved at a concentration (10-4 M) that is difficult to achieve in the in vivo system, except in conditions of overdose and drug abuse. Applied in such a high concentration, the vasodilatory effects of the tested PAMs were comparable to the effects of prazosin, as a reference antagonist of adrenergic contraction. However, their relaxation potential is significantly lower than that of prazosin, as indicated by the observed differences in pEC50 values. For all tested PAMs, the vasodilation effects were more pronounced in FE-precontracted compared to KCl-precontracted preparations...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Vaskularna aktivnost pozitivnih alosternih modulatora GABAA receptora kod pacova
UR  - https://hdl.handle.net/21.15107/rcub_nardus_22506
ER  - 

@phdthesis{
author = "Gajić Bojić, Milica",
year = "2024",
abstract = "U posljednje vrijeme uloga GABA (eng. gamma-aminobutyric acid, GABA) i GABAA receptora (GABAAR) na periferiji postaje sve važnija. Iako su dokazi još uvijek ograničeni, sugerisano je da se hipotenzivni efekti benzodiazepina (BZD) i drugih liganada GABAAR, pored poznatih mehanizama neuromodulacije u centralnom i perifernom nervnom sistemu, ostvaruju i modulacijom "vaskularnih" GABAAR u perifernim krvnim sudovima. Pretpostavili smo da različiti pozitivni alosterni modulatori (PAM) GABAAR ispoljavaju direktne vazodilatatorne efekte preko vaskularnih GABAAR koji su eksprimirani na aorti pacova.Ispitivanja predstavljena u ovoj disertaciji sprovedena su u dva pravca. U jednom pravcu vršeno je dokazivanje prisustva α1-6 i γ2 podjedinica GABAAR u aorti pacova, primjenom RT-PCR (eng. real–time–polymerase chain reaction) i imunohistohemijske analize. U drugom pravcu vršeno je izometrijsko mjerenje kontrakcije izolovanih prstenova pacovske aorte u prisustvu različitih selektivnih i neselektivnih PAM-ova GABAAR: zolpidema (α1–selektivan), XHe–III–074 (α4–selektivan), MP–III–022, MP-III-058, GL-II-74, GL-II-73 (α5–selektivni), DK-I-56-1 (α6–selektivan), SH-I-048A, midazolama i diazepama (neselektivni). Rezultati RT-PCR analize dokazali su ekspresiju iRNK α1–5 podjedinica u homogenatu tkiva torakalne aorte pacova, dok za α6 podjedinicu nije utvrđena ekspresija iRNK. Imunohistohemijskim bojenjem histoloških presjeka pacovske aorte potvrđena je ekspresija proteina α1-5 podjedinice, kao i ekspresija proteina γ2 podjedinice, te je utvrđeno da su identifikovani proteini lokalizovani na vaskularnom glatko-mišićnom sloju aorte pacova. Testovi u kupatilu za rad sa izolovanim prstenovima aorte otkrili su značajne vazodilatacijske efekte svih ispitivanih PAM-ova (preko 50% ostvarene relaksacije prekontrahovanih preparata), pri čemu je diazepam bio najefikasniji ligand, dok je zolpidem pokazao najslabije vaskularne efekte. Flumazenil, kao antagonista BZD mjesta na GABAAR, pokazao je slabu vazoaktivnost per se, ali je značajno smanjio vazodilatacijske efekte testiranih PAM-ova, što upućuje da se njihova vazoaktivnost ostvaruje modulacijom BZD veznog mjesta na vaskularnim GABAAR. Primjenjen u koncentraciji od 10-4 M, flumazenil je značajno smanjio relaksaciju izazvanu midazolamom (P < 0,01), kao i relaksaciju izazvanu MP-III-058 (P < 0,001), i uzrokovao tako pomijeranje njihovih krivih odnosa koncentracija-odgovor udesno i naniže. Vazodilatacijski efekti diazepama nisu smanjeni u prisustvu antagonista (bikukulina, odnosno PK11195), osim onih ostvarenih pri nižim koncentracijama diazepama (10-7 M i 3x10-7 M). TSPO antagonista PK11195 ispoljio je vazodilatacijske efekte na fenilefrin (FE)- prekontrahovanim preparatima, uporedive sa efektima diazepama, što nije bio slučaj sa antagonistima bikukulinom i flumazenilom. Maksimalna efikasnost testiranih PAM-ova postignuta je pri koncentraciji (10-4 M) koju je teško postići u in vivo sistemu, osim u uslovima predoziranja i zloupotrebe lijekova. Primjenjeni u ovako visokoj koncentraciji, vazodilatacijski efekti testiranih PAM-ova bili su uporedivi sa efektima prazosina, kao referentnog antagoniste adrenergičke kontrakciije. Međutim, njihov relaksacijski potencijal značajno je niži od prazosinskog, na šta ukazuju uočene razlike u vrijednostima pEC50. Za sve ispitivane PAM-ove vazodilatacijski efekti bili su izraženiji kod FE- prekontrahovanih u odnosu na KCl- prekontrahovane preparate..., Recently, the role of GABA (gamma-aminobutyric acid) and GABAA receptors (GABAAR) in the periphery has become increasingly important. Although the evidence is still limited, it has been suggested that the hypotensive effects of benzodiazepines (BZD) and other GABAAR ligands, in addition to the known mechanisms of neuromodulation in the central and peripheral nervous system, are also achieved by the modulation of "vascular" GABAAR in peripheral blood vessels. We hypothesized that various positive allosteric modulators (PAM) of GABAAR exert direct vasodilator effects via vascular GABAAR expressed on rat aorta.The investigations presented in this dissertation were carried out in two directions. In one direction, the presence of α and γ2 subunits of the GABAAR in the rat aorta was demonstrated, using RT-PCR (real–time–polymerase chain reaction) and immunohistochemical analysis. In the second direction, isomeric measurement of contraction of isolated rat aortic rings in the presence of various selective and non-selective PAMs of GABAAR was performed: zolpidem (α1–selective), XHe-III-074 (α4–selective), MP-III-022, MP-III-058, GL-II-74, GL-II-73 (α5–selective), DK-I-56-1 (α6–selective), SH-I-048A, midazolam and diazepam (non-selective).The results of RT-PCR analysis demonstrated the expression of mRNA α1–5 subunits in the tissue homogenate of the rat thoracic aorta, while no mRNA expression was found for the α6 subunit. Immunohistochemical staining of histological sections of the rat aorta confirmed the expression of the α1-5 subunit protein, as well as the expression of the γ2 subunit protein, and it was determined that the identified proteins are localized on the vascular smooth-muscle layer of the rat aorta. Assays with isolated aortic rings revealed significant vasodilatory effects of all tested PAMs (over 50% relaxation achieved in precontracted preparation), where diazepam was the most effective ligand, while zolpidem showed the weakest vascular effects. Flumazenil exhibited weak vasoactivity per se, but significantly prevented the relaxant effects of tested PAMs. Applied at a concentration of 10-4 M, flumazenil significantly reduced midazolam-induced relaxation (P < 0.01), as well as MP-III-058-induced relaxation (P < 0.001), shifting their concentration-response curves to the right and down. The vasodilating effects of diazepam were not reduced in the presence of the antagonist (bicuculline, ie PK11195), except for those achieved at lower concentrations of diazepam (10-7 M and 3x10-7 M). The TSPO antagonist PK11195 exhibited vasodilatory effects on phenylephrine (FE)-precontracted preparations, comparable to the effects of diazepam, which was not the case with the antagonists bicuculline and flumazenil. The maximum efficiency of the tested PAMs was achieved at a concentration (10-4 M) that is difficult to achieve in the in vivo system, except in conditions of overdose and drug abuse. Applied in such a high concentration, the vasodilatory effects of the tested PAMs were comparable to the effects of prazosin, as a reference antagonist of adrenergic contraction. However, their relaxation potential is significantly lower than that of prazosin, as indicated by the observed differences in pEC50 values. For all tested PAMs, the vasodilation effects were more pronounced in FE-precontracted compared to KCl-precontracted preparations...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Vaskularna aktivnost pozitivnih alosternih modulatora GABAA receptora kod pacova",
url = "https://hdl.handle.net/21.15107/rcub_nardus_22506"
}

Gajić Bojić, M.. (2024). Vaskularna aktivnost pozitivnih alosternih modulatora GABAA receptora kod pacova. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_22506

Gajić Bojić M. Vaskularna aktivnost pozitivnih alosternih modulatora GABAA receptora kod pacova. in Универзитет у Београду. 2024;.
https://hdl.handle.net/21.15107/rcub_nardus_22506 .

Gajić Bojić, Milica, "Vaskularna aktivnost pozitivnih alosternih modulatora GABAA receptora kod pacova" in Универзитет у Београду (2024),
https://hdl.handle.net/21.15107/rcub_nardus_22506 .

TY  - THES
AU  - Vasić, Marija
PY  - 2024
UR  - https://uvidok.rcub.bg.ac.rs/doccall/bitstream/handle/123456789/5573/Referat.pdf
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9641
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:33770/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/138489353
UR  - https://nardus.mpn.gov.rs/handle/123456789/22507
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5671
AB  - Multipla skleroza (MS) je autoimunska bolest, nepredvidivog toka, sa zonama inflamacije i demijelinizacije u centralnom nervnom sistemu. Cilj istraživanja je bio da se utvrdi klinički značaj oksidativnog stresa (OS) i kinureninskog puta (KP) metabolizma triptofana u nastanku i razvoju relapsno-remitentne multiple skleroze (RRMS) u populaciji Srbije, kao i efekti terapije koja menja prirodni tok bolesti (DMT). Dodatno, cilj je bio da se ispita uticaj sredinskih faktora (duvanski dim, izlaganje suncu i konzumiranje kravljeg mleka) na nastanak i razvoj MS.Istraživanje je obuhvatilo 175 RRMS pacijenata (76 muškaraca i 99 žena) i 254 zdravih osoba (81 muškarac i 173 žene). Za procenu progresije bolesti korišćen je Multiple Sclerosis Severity Score (MSSS). OS je procenjen na osnovu ukupnog antioksidativnog i oksidativnog statusa i oksidativnog indeksa u serumu (TAS, TOS, OSI) i 8-okso-7,8-dihidro-2´-deoksiguanozina (8-oksodG/kreatinin) u urinu. Analiziran je polimorfizam gena za enzim 8-oksoguanin-glikozilazu 1, OGG1 rs1052133 (Ser326Cys). U serumu pacijenata su praćeni laki lanci neurofilamenata (sNfL), sfingozin-1-fosfat (S1P), kinureninska (KA) i hinolinska (QA) kiselina.Rezultati ovog istraživanja su pokazali da OS utiče na nastanak i razvoj MS u populaciji Srbije. Porast OSI, porast 8-oksodG/kreatinina i pasivno pušenje su identifikovani kao nezavisni prediktori za nastanak MS. Dodatno, kod pacijenata sa težim oblikom MS, nivo OS povezanog sa pušenjem bio je veći kod žena, što ukazuje na veće potrebe uključivanja antioksidativne terapije kod žena koje su već obolele od MS, kako bi se prevenirao dalji nepovoljan tok bolesti. Primena TAS, TOS, OSI i 8-oksodG/kreatinina ima potencijalni značaj u proceni rizika od razvoja OS i praćenju efikasnosti antioksidativne terapije. Viši 8-oksodG/kreatinin kod nosilaca C alela rs1052133 u odnosu na GG homozigote bio je povezan sa pojavom MS, što ukazuje na povećanu izloženost sistemskim efektima OS kod nosioca određenog alela genetičke varijante OGG1 rs1052133, ali i na efikasnost sistema reparacije oksidativno modifikovane dezoksiribonukleinske kiseline (DNK). S druge strane, prisustvo C alela kod pacijenata-pušača je bilo povezano sa višim 8-oksodG/kreatinin indeksom u odnosu na nepušače, što ukazuje na moguću genotip-fenotip interakciju u razvoju OS. Dalje, rezultati ove studije ukazuju na zaštitni efekat DMT terapije od pojave OS, međutim, kod žena efekti terapije na prevenciju OS i pojavu teškog oblika MS su bili ograničeni, što ukazuje na neophodnost uvođenja antioksidativne terapije kod žena. Osim toga što su žene, generalno imale viši nivo sNfL, ova studija je pokazala da žene-nosioci G alela rs1052133 imaju viši sNfL u odnosu na žene-homozigote CC, kao i u odnosu na muškarce-nosioce G alela. Dodatno, žene su nakon šestomesečne DMT terapije imale značajno veći porast sNfL u odnosu na muškarce, što može ukazati da njihova veća osetljivost na oštećenje aksona zahteva duži vremenski period za postizanje pozitivnih efekata terapije. Povećana produkcija hinolinske kiseline bila je povezana sa rizikom od progresije MS kod žena, što ukazuje da hronična aktivacija KP povećava rizik od neurodegeneracije. Nakon šestomesečne DMT terapije, kod oba pola uočen je značajan pad indeksa QA/KA, što ukazuje na njen zaštitni efekat od nakupljanja neurotoksičnih metabolita i ovaj indeks bi mogao da bude koristan za procenu rizika od progresije MS...
AB  - Multiple sclerosis (MS) is an autoimmune disease with an unpredictable course, with zones of inflammation and demyelination in the central nervous system. The aim of the research was to determine the clinical significance of oxidative stress (OS) and the kynurenine pathway (KP) of tryptophan metabolism in the onset and development of relapsing-remitting multiple sclerosis (RRMS) in the population of Serbia, as well as the effects of therapy that changes the natural course of the disease (DMT). Additionally, the aim was to examine the influence of environmental factors (tobacco smoke, sun exposure and consumption of cow's milk) on the onset and development of MS.The research included 175 RRMS patients (76 men and 99 women) and 254 healthy individuals (81 men and 173 women). The Multiple Sclerosis Severity Score (MSSS) was used to assess the severity of the disease. OS was evaluated based on total antioxidant and oxidative status and oxidative index in serum (TAS, TOS, OSI), and 8-oxo-7,8-dihydro-2´-deoxyguanosine (8-oxodG/creatinine) in urine. The gene polymorphism of the enzyme 8-oxoguanine-glycosylase 1, OGG1 rs1052133 (Ser326Cys) was analyzed. Neurofilament light chains (sNfL), sphingosine-1-phosphate (S1P), kynurenic (KA) and quinolinic (QA) acid were monitored in the patients' serum.The results of this research showed that OS affects the onset and development of MS in the population of Serbia. Increase in OSI, increase in 8-oxodG/creatinine and passive smoking were identified as independent predictors for the development of MS. Additionally, in patients with a more severe form of MS, the level of OS-related to smoking was higher in women, which indicates a greater need to include antioxidant therapy in women already suffering from MS, in order to prevent further adverse course of the disease. The application of TAS, TOS, OSI and 8-oxodG/creatinine has potential importance in assessing the risk of developing OS and monitoring the effectiveness of antioxidant therapy. Higher 8-oxodG/creatinine in rs1052133 C allele carriers compared to GG homozygotes was associated with the occurrence of MS, which indicates increased exposure to systemic effects of OS related to certain allele of genetic variant OGG1 rs1052133, but also the efficiency of the repair system of oxidatively modified DNA. On the other hand, the presence of the rs1052133 C allele in smoking patients was associated with higher 8-oxodG/creatinine compared to non-smokers, indicating a possible genotype-phenotype interaction in the development of OS. Furthermore, the results of this study indicate a protective effect of DMT therapy against the occurrence of OS, however, in women, the effects of the therapy on the prevention of OS and the occurrence of severe MS were limited, which indicates the necessity of introducing antioxidant therapy in women. In addition to women generally having higher sNfL, this study showed that female carriers of the rs1052133 G allele have higher sNfL compared to homozygous CC women, as well as compared to male carriers of the G allele. After six months of DMT therapy, women had a significantly greater increase in sNfL compared to men, which may indicate that their greater sensitivity to axonal damage requires a longer period of time to achieve positive effects of therapy. Increased production of quinolinic acid was associated with the risk of MS progression in women, indicating that chronic KPactivation increases the risk of neurodegeneration. After six months of DMT therapy, a significant decrease in the QA/KA index was observed in both sexes, indicating its protective effect against the accumulation of neurotoxic metabolites, and this index could be useful for assessing the risk of MS progression...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Značaj oksidativnog stresa i kinureninskog puta metabolizma triptofana u nastanku i razvoju multiple skleroze u populaciji Srbije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_22507
ER  - 

@phdthesis{
author = "Vasić, Marija",
year = "2024",
abstract = "Multipla skleroza (MS) je autoimunska bolest, nepredvidivog toka, sa zonama inflamacije i demijelinizacije u centralnom nervnom sistemu. Cilj istraživanja je bio da se utvrdi klinički značaj oksidativnog stresa (OS) i kinureninskog puta (KP) metabolizma triptofana u nastanku i razvoju relapsno-remitentne multiple skleroze (RRMS) u populaciji Srbije, kao i efekti terapije koja menja prirodni tok bolesti (DMT). Dodatno, cilj je bio da se ispita uticaj sredinskih faktora (duvanski dim, izlaganje suncu i konzumiranje kravljeg mleka) na nastanak i razvoj MS.Istraživanje je obuhvatilo 175 RRMS pacijenata (76 muškaraca i 99 žena) i 254 zdravih osoba (81 muškarac i 173 žene). Za procenu progresije bolesti korišćen je Multiple Sclerosis Severity Score (MSSS). OS je procenjen na osnovu ukupnog antioksidativnog i oksidativnog statusa i oksidativnog indeksa u serumu (TAS, TOS, OSI) i 8-okso-7,8-dihidro-2´-deoksiguanozina (8-oksodG/kreatinin) u urinu. Analiziran je polimorfizam gena za enzim 8-oksoguanin-glikozilazu 1, OGG1 rs1052133 (Ser326Cys). U serumu pacijenata su praćeni laki lanci neurofilamenata (sNfL), sfingozin-1-fosfat (S1P), kinureninska (KA) i hinolinska (QA) kiselina.Rezultati ovog istraživanja su pokazali da OS utiče na nastanak i razvoj MS u populaciji Srbije. Porast OSI, porast 8-oksodG/kreatinina i pasivno pušenje su identifikovani kao nezavisni prediktori za nastanak MS. Dodatno, kod pacijenata sa težim oblikom MS, nivo OS povezanog sa pušenjem bio je veći kod žena, što ukazuje na veće potrebe uključivanja antioksidativne terapije kod žena koje su već obolele od MS, kako bi se prevenirao dalji nepovoljan tok bolesti. Primena TAS, TOS, OSI i 8-oksodG/kreatinina ima potencijalni značaj u proceni rizika od razvoja OS i praćenju efikasnosti antioksidativne terapije. Viši 8-oksodG/kreatinin kod nosilaca C alela rs1052133 u odnosu na GG homozigote bio je povezan sa pojavom MS, što ukazuje na povećanu izloženost sistemskim efektima OS kod nosioca određenog alela genetičke varijante OGG1 rs1052133, ali i na efikasnost sistema reparacije oksidativno modifikovane dezoksiribonukleinske kiseline (DNK). S druge strane, prisustvo C alela kod pacijenata-pušača je bilo povezano sa višim 8-oksodG/kreatinin indeksom u odnosu na nepušače, što ukazuje na moguću genotip-fenotip interakciju u razvoju OS. Dalje, rezultati ove studije ukazuju na zaštitni efekat DMT terapije od pojave OS, međutim, kod žena efekti terapije na prevenciju OS i pojavu teškog oblika MS su bili ograničeni, što ukazuje na neophodnost uvođenja antioksidativne terapije kod žena. Osim toga što su žene, generalno imale viši nivo sNfL, ova studija je pokazala da žene-nosioci G alela rs1052133 imaju viši sNfL u odnosu na žene-homozigote CC, kao i u odnosu na muškarce-nosioce G alela. Dodatno, žene su nakon šestomesečne DMT terapije imale značajno veći porast sNfL u odnosu na muškarce, što može ukazati da njihova veća osetljivost na oštećenje aksona zahteva duži vremenski period za postizanje pozitivnih efekata terapije. Povećana produkcija hinolinske kiseline bila je povezana sa rizikom od progresije MS kod žena, što ukazuje da hronična aktivacija KP povećava rizik od neurodegeneracije. Nakon šestomesečne DMT terapije, kod oba pola uočen je značajan pad indeksa QA/KA, što ukazuje na njen zaštitni efekat od nakupljanja neurotoksičnih metabolita i ovaj indeks bi mogao da bude koristan za procenu rizika od progresije MS..., Multiple sclerosis (MS) is an autoimmune disease with an unpredictable course, with zones of inflammation and demyelination in the central nervous system. The aim of the research was to determine the clinical significance of oxidative stress (OS) and the kynurenine pathway (KP) of tryptophan metabolism in the onset and development of relapsing-remitting multiple sclerosis (RRMS) in the population of Serbia, as well as the effects of therapy that changes the natural course of the disease (DMT). Additionally, the aim was to examine the influence of environmental factors (tobacco smoke, sun exposure and consumption of cow's milk) on the onset and development of MS.The research included 175 RRMS patients (76 men and 99 women) and 254 healthy individuals (81 men and 173 women). The Multiple Sclerosis Severity Score (MSSS) was used to assess the severity of the disease. OS was evaluated based on total antioxidant and oxidative status and oxidative index in serum (TAS, TOS, OSI), and 8-oxo-7,8-dihydro-2´-deoxyguanosine (8-oxodG/creatinine) in urine. The gene polymorphism of the enzyme 8-oxoguanine-glycosylase 1, OGG1 rs1052133 (Ser326Cys) was analyzed. Neurofilament light chains (sNfL), sphingosine-1-phosphate (S1P), kynurenic (KA) and quinolinic (QA) acid were monitored in the patients' serum.The results of this research showed that OS affects the onset and development of MS in the population of Serbia. Increase in OSI, increase in 8-oxodG/creatinine and passive smoking were identified as independent predictors for the development of MS. Additionally, in patients with a more severe form of MS, the level of OS-related to smoking was higher in women, which indicates a greater need to include antioxidant therapy in women already suffering from MS, in order to prevent further adverse course of the disease. The application of TAS, TOS, OSI and 8-oxodG/creatinine has potential importance in assessing the risk of developing OS and monitoring the effectiveness of antioxidant therapy. Higher 8-oxodG/creatinine in rs1052133 C allele carriers compared to GG homozygotes was associated with the occurrence of MS, which indicates increased exposure to systemic effects of OS related to certain allele of genetic variant OGG1 rs1052133, but also the efficiency of the repair system of oxidatively modified DNA. On the other hand, the presence of the rs1052133 C allele in smoking patients was associated with higher 8-oxodG/creatinine compared to non-smokers, indicating a possible genotype-phenotype interaction in the development of OS. Furthermore, the results of this study indicate a protective effect of DMT therapy against the occurrence of OS, however, in women, the effects of the therapy on the prevention of OS and the occurrence of severe MS were limited, which indicates the necessity of introducing antioxidant therapy in women. In addition to women generally having higher sNfL, this study showed that female carriers of the rs1052133 G allele have higher sNfL compared to homozygous CC women, as well as compared to male carriers of the G allele. After six months of DMT therapy, women had a significantly greater increase in sNfL compared to men, which may indicate that their greater sensitivity to axonal damage requires a longer period of time to achieve positive effects of therapy. Increased production of quinolinic acid was associated with the risk of MS progression in women, indicating that chronic KPactivation increases the risk of neurodegeneration. After six months of DMT therapy, a significant decrease in the QA/KA index was observed in both sexes, indicating its protective effect against the accumulation of neurotoxic metabolites, and this index could be useful for assessing the risk of MS progression...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Značaj oksidativnog stresa i kinureninskog puta metabolizma triptofana u nastanku i razvoju multiple skleroze u populaciji Srbije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_22507"
}

Vasić, M.. (2024). Značaj oksidativnog stresa i kinureninskog puta metabolizma triptofana u nastanku i razvoju multiple skleroze u populaciji Srbije. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_22507

Vasić M. Značaj oksidativnog stresa i kinureninskog puta metabolizma triptofana u nastanku i razvoju multiple skleroze u populaciji Srbije. in Универзитет у Београду. 2024;.
https://hdl.handle.net/21.15107/rcub_nardus_22507 .

Vasić, Marija, "Značaj oksidativnog stresa i kinureninskog puta metabolizma triptofana u nastanku i razvoju multiple skleroze u populaciji Srbije" in Универзитет у Београду (2024),
https://hdl.handle.net/21.15107/rcub_nardus_22507 .

TY  - JOUR
AU  - Kovačević, Peđa
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Barišić, Vedrana
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
AU  - Rizwan, Zeeshan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5631
AB  - Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Naunyn-Schmiedeberg's Archives of Pharmacology
T1  - Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study
DO  - 10.1007/s00210-024-03136-1
ER  - 

@article{
author = "Kovačević, Peđa and Milaković, Dragana and Kovačević, Tijana and Barišić, Vedrana and Dragić, Saša and Zlojutro, Biljana and Miljković, Branislava and Vučićević, Katarina and Rizwan, Zeeshan",
year = "2024",
abstract = "Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Naunyn-Schmiedeberg's Archives of Pharmacology",
title = "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study",
doi = "10.1007/s00210-024-03136-1"
}

Kovačević, P., Milaković, D., Kovačević, T., Barišić, V., Dragić, S., Zlojutro, B., Miljković, B., Vučićević, K.,& Rizwan, Z.. (2024). Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00210-024-03136-1

Kovačević P, Milaković D, Kovačević T, Barišić V, Dragić S, Zlojutro B, Miljković B, Vučićević K, Rizwan Z. Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology. 2024;.
doi:10.1007/s00210-024-03136-1 .

Kovačević, Peđa, Milaković, Dragana, Kovačević, Tijana, Barišić, Vedrana, Dragić, Saša, Zlojutro, Biljana, Miljković, Branislava, Vučićević, Katarina, Rizwan, Zeeshan, "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study" in Naunyn-Schmiedeberg's Archives of Pharmacology (2024),
https://doi.org/10.1007/s00210-024-03136-1 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
year = "2024",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I.. (2024). Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines. 2024;12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4 (2024),
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Adamov, Ivana
AU  - Stanojević, Gordana
AU  - Pavlović, Stefan
AU  - Medarević, Đorđe
AU  - Ivković, Branka
AU  - Kočović, David
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5630
AB  - Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets
VL  - 657
DO  - 10.1016/j.ijpharm.2024.124161
ER  - 

@article{
author = "Adamov, Ivana and Stanojević, Gordana and Pavlović, Stefan and Medarević, Đorđe and Ivković, Branka and Kočović, David and Ibrić, Svetlana",
year = "2024",
abstract = "Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets",
volume = "657",
doi = "10.1016/j.ijpharm.2024.124161"
}

Adamov, I., Stanojević, G., Pavlović, S., Medarević, Đ., Ivković, B., Kočović, D.,& Ibrić, S.. (2024). Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics
Elsevier B.V.., 657.
https://doi.org/10.1016/j.ijpharm.2024.124161

Adamov I, Stanojević G, Pavlović S, Medarević Đ, Ivković B, Kočović D, Ibrić S. Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics. 2024;657.
doi:10.1016/j.ijpharm.2024.124161 .

Adamov, Ivana, Stanojević, Gordana, Pavlović, Stefan, Medarević, Đorđe, Ivković, Branka, Kočović, David, Ibrić, Svetlana, "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets" in International Journal of Pharmaceutics, 657 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124161 . .

TY  - CONF
AU  - Zečević, Anđelka
AU  - Ćulafić, Milica
AU  - Stojković, Stefan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5660
AB  - The simplified information page (SIP) is a simplified discharge summary created to mitigate health risks caused by low medical comprehension. One of the most critical aspects of medical comprehension concerns interpreting medication instructions such as proper dosing, frequency, and duration. In our work, we examine the capacities of mainstream Large Language Models (LLMs) such as ChatGPT and Gemini to generate SIP-like medication-oriented pages based on the provided discharge summaries. We are sharing the initial qualitative assessments of our study based on a small collection of discharge summaries in Serbian, pointing to noticed inaccuracies, unfaithful content, and language quality. Hopefully, these findings might be helpful in addressing the multilingual perspective of patient-oriented language.
PB  - European Language Resources Association (ELRA)
C3  - Proceedings of the First Workshop on Patient-Oriented Language Processing @LREC-COLING-2024 (CL4Health), 20 May, 2024 Torino, Italia
T1  - On Simplification of Discharge Summaries in Serbian: Facing the Challenges
SP  - 104
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5660
ER  - 

@conference{
author = "Zečević, Anđelka and Ćulafić, Milica and Stojković, Stefan",
year = "2024",
abstract = "The simplified information page (SIP) is a simplified discharge summary created to mitigate health risks caused by low medical comprehension. One of the most critical aspects of medical comprehension concerns interpreting medication instructions such as proper dosing, frequency, and duration. In our work, we examine the capacities of mainstream Large Language Models (LLMs) such as ChatGPT and Gemini to generate SIP-like medication-oriented pages based on the provided discharge summaries. We are sharing the initial qualitative assessments of our study based on a small collection of discharge summaries in Serbian, pointing to noticed inaccuracies, unfaithful content, and language quality. Hopefully, these findings might be helpful in addressing the multilingual perspective of patient-oriented language.",
publisher = "European Language Resources Association (ELRA)",
journal = "Proceedings of the First Workshop on Patient-Oriented Language Processing @LREC-COLING-2024 (CL4Health), 20 May, 2024 Torino, Italia",
title = "On Simplification of Discharge Summaries in Serbian: Facing the Challenges",
pages = "104-108",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5660"
}

Zečević, A., Ćulafić, M.,& Stojković, S.. (2024). On Simplification of Discharge Summaries in Serbian: Facing the Challenges. in Proceedings of the First Workshop on Patient-Oriented Language Processing @LREC-COLING-2024 (CL4Health), 20 May, 2024 Torino, Italia
European Language Resources Association (ELRA)., 104-108.
https://hdl.handle.net/21.15107/rcub_farfar_5660

Zečević A, Ćulafić M, Stojković S. On Simplification of Discharge Summaries in Serbian: Facing the Challenges. in Proceedings of the First Workshop on Patient-Oriented Language Processing @LREC-COLING-2024 (CL4Health), 20 May, 2024 Torino, Italia. 2024;:104-108.
https://hdl.handle.net/21.15107/rcub_farfar_5660 .

Zečević, Anđelka, Ćulafić, Milica, Stojković, Stefan, "On Simplification of Discharge Summaries in Serbian: Facing the Challenges" in Proceedings of the First Workshop on Patient-Oriented Language Processing @LREC-COLING-2024 (CL4Health), 20 May, 2024 Torino, Italia (2024):104-108,
https://hdl.handle.net/21.15107/rcub_farfar_5660 .

TY  - JOUR
AU  - Marić, Đurđica
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Milošević, Ivan
AU  - Nikolić, Anja
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Aschner, Michael
AU  - Buha-Đorđević, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5659
AB  - This study explored the effect of a toxic metal(oid) mixture (cadmium, lead, arsenic, mercury, chromium, and nickel) on thyroid function in Wistar rats exposed for 28 or 90 days. Dose levels were determined based on prior human-biomonitoring investigation. The experiment included control (male/female rats, 28 and 90 days) and treated groups, reflecting the lower confidence limit of the Benchmark Dose (BMDL) for hormone levels (M1/F1, 28 and 90 days), median concentrations (M2/F2, 28 and 90 days), 95th percentile concentrations (M3/F3, 28 and 90 days) measured in a human study, and reference values for individual metals extracted from the literature (M4/F4, 28 days only). Blood and thyroid gland samples were collected at the experimental termination. Serum TSH, fT3, fT4, T3, and T4 levels were measured, and SPINA-GT and SPINA-GD parameters were calculated. In silico analysis, employing the Comparative Toxicogenomic Database and ToppGene Suite portal, aimed to reveal molecular mechanisms underlying the observed effects. Results showed greater sensitivity in the female rats, with significant effects observed at lower doses. Subacute exposure increased TSH, fT3, and T3 levels in females, while subchronic exposure in males decreased TSH and fT3 levels and increased fT4. Subacute exposure induced changes even at allegedly safe doses, emphasizing potential health risks. Histological abnormalities were observed in all the treated groups. In silico findings suggested that toxic metal exposure contributes to thyroid disorders via oxidative stress, disruption of micronutrients, interference with hormone synthesis, and gene expression dysregulation. These results indicate that seemingly safe doses in single-substance research can adversely affect thyroid structure and function when administered as a mixture. These findings highlight the complex impact of toxic metal exposure on thyroid health, emphasizing that adhering to accepted safety limits for single-substance research fails to account for adverse effects on thyroid structure and function upon exposures to metal mixtures.
PB  - Elsevier Ltd
T2  - Chemosphere
T1  - Thyroid under siege: Unravelling the toxic impact of real-life metal mixture exposures in Wistar rats
VL  - 360
DO  - 10.1016/j.chemosphere.2024.142441
ER  - 

@article{
author = "Marić, Đurđica and Baralić, Katarina and Vukelić, Dragana and Milošević, Ivan and Nikolić, Anja and Antonijević, Biljana and Đukić-Ćosić, Danijela and Bulat, Zorica and Aschner, Michael and Buha-Đorđević, Aleksandra",
year = "2024",
abstract = "This study explored the effect of a toxic metal(oid) mixture (cadmium, lead, arsenic, mercury, chromium, and nickel) on thyroid function in Wistar rats exposed for 28 or 90 days. Dose levels were determined based on prior human-biomonitoring investigation. The experiment included control (male/female rats, 28 and 90 days) and treated groups, reflecting the lower confidence limit of the Benchmark Dose (BMDL) for hormone levels (M1/F1, 28 and 90 days), median concentrations (M2/F2, 28 and 90 days), 95th percentile concentrations (M3/F3, 28 and 90 days) measured in a human study, and reference values for individual metals extracted from the literature (M4/F4, 28 days only). Blood and thyroid gland samples were collected at the experimental termination. Serum TSH, fT3, fT4, T3, and T4 levels were measured, and SPINA-GT and SPINA-GD parameters were calculated. In silico analysis, employing the Comparative Toxicogenomic Database and ToppGene Suite portal, aimed to reveal molecular mechanisms underlying the observed effects. Results showed greater sensitivity in the female rats, with significant effects observed at lower doses. Subacute exposure increased TSH, fT3, and T3 levels in females, while subchronic exposure in males decreased TSH and fT3 levels and increased fT4. Subacute exposure induced changes even at allegedly safe doses, emphasizing potential health risks. Histological abnormalities were observed in all the treated groups. In silico findings suggested that toxic metal exposure contributes to thyroid disorders via oxidative stress, disruption of micronutrients, interference with hormone synthesis, and gene expression dysregulation. These results indicate that seemingly safe doses in single-substance research can adversely affect thyroid structure and function when administered as a mixture. These findings highlight the complex impact of toxic metal exposure on thyroid health, emphasizing that adhering to accepted safety limits for single-substance research fails to account for adverse effects on thyroid structure and function upon exposures to metal mixtures.",
publisher = "Elsevier Ltd",
journal = "Chemosphere",
title = "Thyroid under siege: Unravelling the toxic impact of real-life metal mixture exposures in Wistar rats",
volume = "360",
doi = "10.1016/j.chemosphere.2024.142441"
}

Marić, Đ., Baralić, K., Vukelić, D., Milošević, I., Nikolić, A., Antonijević, B., Đukić-Ćosić, D., Bulat, Z., Aschner, M.,& Buha-Đorđević, A.. (2024). Thyroid under siege: Unravelling the toxic impact of real-life metal mixture exposures in Wistar rats. in Chemosphere
Elsevier Ltd., 360.
https://doi.org/10.1016/j.chemosphere.2024.142441

Marić Đ, Baralić K, Vukelić D, Milošević I, Nikolić A, Antonijević B, Đukić-Ćosić D, Bulat Z, Aschner M, Buha-Đorđević A. Thyroid under siege: Unravelling the toxic impact of real-life metal mixture exposures in Wistar rats. in Chemosphere. 2024;360.
doi:10.1016/j.chemosphere.2024.142441 .

Marić, Đurđica, Baralić, Katarina, Vukelić, Dragana, Milošević, Ivan, Nikolić, Anja, Antonijević, Biljana, Đukić-Ćosić, Danijela, Bulat, Zorica, Aschner, Michael, Buha-Đorđević, Aleksandra, "Thyroid under siege: Unravelling the toxic impact of real-life metal mixture exposures in Wistar rats" in Chemosphere, 360 (2024),
https://doi.org/10.1016/j.chemosphere.2024.142441 . .

TY  - JOUR
AU  - Jovanović, Aleksandar
AU  - Drobac, Milica
AU  - Vidović, Bojana
AU  - Pavlović, Dragana
AU  - Krajnović, Dušanka
AU  - Tadić, Ivana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5658
AB  - ntroduction: Urinary tract infections (UTIs) are one of the most common bacterial infections accompanied by inflammation in any part of the urinary system. Antibiotics are the gold standard for UTI treatment; however, herbal products are also administered. The study aimed to assess patients' attitudes and opinions regarding the use and effectiveness of antibiotics and herbal products for the treatment of UTIs. Methods: A quantitative, non-experimental study was conducted using an online survey in the Republic of Serbia in 2021 and 2022. Results: The study comprised 393 respondents, with average age of 34.66 years and most respondents being women (91.9%). According to the personal assessment, slightly less than half of the respondents (46.1%) classified UTI symptoms as severe. Respondents predominantly treated UTI symptoms with herbal products combined with antibiotics (52.2%) or herbal products alone (26.6%). The most frequently used antibiotics were ciprofloxacin (20.9%), cephalexin (16.9%), and sulfamethoxazole/trimethoprim (15.4%). As many as 74.3% of respondents used herbal teas. The most used herbal products contained bearberry leaf (Uvae ursi folium) (52.1%), parsley leaf/root (Petroselini foium/radix) (15.1%), and cranberry fruit (Vaccinii macrocarpi fructus) (12.6%). Self-assessment of the effectiveness of UTI treatment with antibiotics and herbal products was high. Conclusion: The antibiotics used to treat UTIs did not align with the guidelines of the European Association of Urologists. Further, the use of herbal products was frequently observed. Therefore, community pharmacists, who serve as the most accessible healthcare professionals with competent knowledge about herbal products, should adequately inform patients about potential therapeutic options for UTI.
PB  - Elsevier B.V.
T2  - Journal of Herbal Medicine
T1  - Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes
VL  - 46
DO  - 10.1016/j.hermed.2024.100892
ER  - 

@article{
author = "Jovanović, Aleksandar and Drobac, Milica and Vidović, Bojana and Pavlović, Dragana and Krajnović, Dušanka and Tadić, Ivana",
year = "2024",
abstract = "ntroduction: Urinary tract infections (UTIs) are one of the most common bacterial infections accompanied by inflammation in any part of the urinary system. Antibiotics are the gold standard for UTI treatment; however, herbal products are also administered. The study aimed to assess patients' attitudes and opinions regarding the use and effectiveness of antibiotics and herbal products for the treatment of UTIs. Methods: A quantitative, non-experimental study was conducted using an online survey in the Republic of Serbia in 2021 and 2022. Results: The study comprised 393 respondents, with average age of 34.66 years and most respondents being women (91.9%). According to the personal assessment, slightly less than half of the respondents (46.1%) classified UTI symptoms as severe. Respondents predominantly treated UTI symptoms with herbal products combined with antibiotics (52.2%) or herbal products alone (26.6%). The most frequently used antibiotics were ciprofloxacin (20.9%), cephalexin (16.9%), and sulfamethoxazole/trimethoprim (15.4%). As many as 74.3% of respondents used herbal teas. The most used herbal products contained bearberry leaf (Uvae ursi folium) (52.1%), parsley leaf/root (Petroselini foium/radix) (15.1%), and cranberry fruit (Vaccinii macrocarpi fructus) (12.6%). Self-assessment of the effectiveness of UTI treatment with antibiotics and herbal products was high. Conclusion: The antibiotics used to treat UTIs did not align with the guidelines of the European Association of Urologists. Further, the use of herbal products was frequently observed. Therefore, community pharmacists, who serve as the most accessible healthcare professionals with competent knowledge about herbal products, should adequately inform patients about potential therapeutic options for UTI.",
publisher = "Elsevier B.V.",
journal = "Journal of Herbal Medicine",
title = "Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes",
volume = "46",
doi = "10.1016/j.hermed.2024.100892"
}

Jovanović, A., Drobac, M., Vidović, B., Pavlović, D., Krajnović, D.,& Tadić, I.. (2024). Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes. in Journal of Herbal Medicine
Elsevier B.V.., 46.
https://doi.org/10.1016/j.hermed.2024.100892

Jovanović A, Drobac M, Vidović B, Pavlović D, Krajnović D, Tadić I. Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes. in Journal of Herbal Medicine. 2024;46.
doi:10.1016/j.hermed.2024.100892 .

Jovanović, Aleksandar, Drobac, Milica, Vidović, Bojana, Pavlović, Dragana, Krajnović, Dušanka, Tadić, Ivana, "Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes" in Journal of Herbal Medicine, 46 (2024),
https://doi.org/10.1016/j.hermed.2024.100892 . .

TY  - JOUR
AU  - Ćurčić, Vladimir
AU  - Olszewski, Mateusz
AU  - Maciejewska, Natalia
AU  - Višnjevac, Aleksandar
AU  - Srdić-Rajić, Tatjana
AU  - Dobričić, Vladimir
AU  - García-Sosa, Alfonso T.
AU  - Kokanov, Sanja B.
AU  - Araškov, Jovana B.
AU  - Silvestri, Romano
AU  - Schüle, Roland
AU  - Jung, Manfred
AU  - Nikolić, Milan
AU  - Filipović, Nenad R.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5298
AB  - Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.
PB  - John Wiley and Sons Inc
T2  - Archiv der Pharmazie
T1  - Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition
VL  - 357
IS  - 2
SP  - 2300426
DO  - 10.1002/ardp.202300426
ER  - 

@article{
author = "Ćurčić, Vladimir and Olszewski, Mateusz and Maciejewska, Natalia and Višnjevac, Aleksandar and Srdić-Rajić, Tatjana and Dobričić, Vladimir and García-Sosa, Alfonso T. and Kokanov, Sanja B. and Araškov, Jovana B. and Silvestri, Romano and Schüle, Roland and Jung, Manfred and Nikolić, Milan and Filipović, Nenad R.",
year = "2024",
abstract = "Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.",
publisher = "John Wiley and Sons Inc",
journal = "Archiv der Pharmazie",
title = "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition",
volume = "357",
number = "2",
pages = "2300426",
doi = "10.1002/ardp.202300426"
}

Ćurčić, V., Olszewski, M., Maciejewska, N., Višnjevac, A., Srdić-Rajić, T., Dobričić, V., García-Sosa, A. T., Kokanov, S. B., Araškov, J. B., Silvestri, R., Schüle, R., Jung, M., Nikolić, M.,& Filipović, N. R.. (2024). Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie
John Wiley and Sons Inc., 357(2), 2300426.
https://doi.org/10.1002/ardp.202300426

Ćurčić V, Olszewski M, Maciejewska N, Višnjevac A, Srdić-Rajić T, Dobričić V, García-Sosa AT, Kokanov SB, Araškov JB, Silvestri R, Schüle R, Jung M, Nikolić M, Filipović NR. Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie. 2024;357(2):2300426.
doi:10.1002/ardp.202300426 .

Ćurčić, Vladimir, Olszewski, Mateusz, Maciejewska, Natalia, Višnjevac, Aleksandar, Srdić-Rajić, Tatjana, Dobričić, Vladimir, García-Sosa, Alfonso T., Kokanov, Sanja B., Araškov, Jovana B., Silvestri, Romano, Schüle, Roland, Jung, Manfred, Nikolić, Milan, Filipović, Nenad R., "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition" in Archiv der Pharmazie, 357, no. 2 (2024):2300426,
https://doi.org/10.1002/ardp.202300426 . .

TY  - JOUR
AU  - Skalny, Anatoly V.
AU  - Aschner, Michael
AU  - Zhang, Feng
AU  - Guo, Xiong
AU  - Buha-Đorđević, Aleksandra
AU  - Sotnikova, Tatiana I.
AU  - Korobeinikova, Tatiana V.
AU  - Domingo, Jose L.
AU  - Farsky, Sandra H. P.
AU  - Tinkov, Alexey A.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5645
AB  - The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Archives of Toxicology
T1  - Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis
DO  - 10.1007/s00204-024-03772-9
ER  - 

@article{
author = "Skalny, Anatoly V. and Aschner, Michael and Zhang, Feng and Guo, Xiong and Buha-Đorđević, Aleksandra and Sotnikova, Tatiana I. and Korobeinikova, Tatiana V. and Domingo, Jose L. and Farsky, Sandra H. P. and Tinkov, Alexey A.",
year = "2024",
abstract = "The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Archives of Toxicology",
title = "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis",
doi = "10.1007/s00204-024-03772-9"
}

Skalny, A. V., Aschner, M., Zhang, F., Guo, X., Buha-Đorđević, A., Sotnikova, T. I., Korobeinikova, T. V., Domingo, J. L., Farsky, S. H. P.,& Tinkov, A. A.. (2024). Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00204-024-03772-9

Skalny AV, Aschner M, Zhang F, Guo X, Buha-Đorđević A, Sotnikova TI, Korobeinikova TV, Domingo JL, Farsky SHP, Tinkov AA. Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology. 2024;.
doi:10.1007/s00204-024-03772-9 .

Skalny, Anatoly V., Aschner, Michael, Zhang, Feng, Guo, Xiong, Buha-Đorđević, Aleksandra, Sotnikova, Tatiana I., Korobeinikova, Tatiana V., Domingo, Jose L., Farsky, Sandra H. P., Tinkov, Alexey A., "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis" in Archives of Toxicology (2024),
https://doi.org/10.1007/s00204-024-03772-9 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Milinković Budinčić, Jelena
AU  - Stanić, Dušanka
AU  - Fraj, Jadranka
AU  - Petrović, Lidija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5647
AB  - The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.
PB  - MDPI
T2  - Pharmaceutics
T1  - Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050628
ER  - 

@article{
author = "Đekić, Ljiljana and Milinković Budinčić, Jelena and Stanić, Dušanka and Fraj, Jadranka and Petrović, Lidija",
year = "2024",
abstract = "The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050628"
}

Đekić, L., Milinković Budinčić, J., Stanić, D., Fraj, J.,& Petrović, L.. (2024). Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050628

Đekić L, Milinković Budinčić J, Stanić D, Fraj J, Petrović L. Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050628 .

Đekić, Ljiljana, Milinković Budinčić, Jelena, Stanić, Dušanka, Fraj, Jadranka, Petrović, Lidija, "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050628 . .

TY  - JOUR
AU  - Simić, Ivana
AU  - Guzonjić, Azra
AU  - Kotur-Stevuljević, Jelena
AU  - Ćeriman Krstić, Vesna
AU  - Samardžić, Natalija
AU  - Savić Vujović, Katarina
AU  - Jovanović, Dragana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5648
AB  - Background and objectives: The objective of this research was to analyze the correlation of the neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), soluble programmed cell death ligand 1 (sPD-L1), and Schlafen 11 (SLFN11) with the response to first-line chemotherapy in a cohort of small cell lung cancer (SCLC) patients, and to determine their potential as predictive serum biomarkers. Materials and Methods: A total of 60 SCLC patients were included. Blood samples were taken to determine CRP, sPD-L1, and SLFN11 levels. The first sampling was performed before the start of chemotherapy, the second after two cycles, and the third after four cycles of chemotherapy. Results: The patients who died earlier during the study had NLR and SLFN11 concentrations significantly higher compared to the survivor group. In the group of survivors, after two cycles of chemotherapy, the NLR ratio decreased significantly (p < 0.01), but after four cycles, the NLR ratio increased (p < 0.05). Their serum SLFN11 concentration increased significantly (p < 0.001) after two cycles of chemotherapy, but after four cycles, the level of SLFN11 fell significantly (p < 0.01). CRP, NLR, and SLFN11 were significant predictors of patient survival according to Kaplan–Meier analysis. The combination of inflammatory parameters and SLFN11 with a cutoff value above the 75th percentile of the predicted probability was associated with significantly lower overall survival in SCLC patients (average survival of 3.6 months vs. 4.8 months). Conclusion: The combination of inflammatory markers and the levels of two specific proteins (sPD-L1, SLFN11) could potentially serve as a non-invasive biomarker for predicting responses to DNA-damaging therapeutic agents in SCLC.
PB  - MDPI
T2  - Biomedicines
T1  - Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study
VL  - 12
IS  - 5
DO  - 10.3390/biomedicines12050976
ER  - 

@article{
author = "Simić, Ivana and Guzonjić, Azra and Kotur-Stevuljević, Jelena and Ćeriman Krstić, Vesna and Samardžić, Natalija and Savić Vujović, Katarina and Jovanović, Dragana",
year = "2024",
abstract = "Background and objectives: The objective of this research was to analyze the correlation of the neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), soluble programmed cell death ligand 1 (sPD-L1), and Schlafen 11 (SLFN11) with the response to first-line chemotherapy in a cohort of small cell lung cancer (SCLC) patients, and to determine their potential as predictive serum biomarkers. Materials and Methods: A total of 60 SCLC patients were included. Blood samples were taken to determine CRP, sPD-L1, and SLFN11 levels. The first sampling was performed before the start of chemotherapy, the second after two cycles, and the third after four cycles of chemotherapy. Results: The patients who died earlier during the study had NLR and SLFN11 concentrations significantly higher compared to the survivor group. In the group of survivors, after two cycles of chemotherapy, the NLR ratio decreased significantly (p < 0.01), but after four cycles, the NLR ratio increased (p < 0.05). Their serum SLFN11 concentration increased significantly (p < 0.001) after two cycles of chemotherapy, but after four cycles, the level of SLFN11 fell significantly (p < 0.01). CRP, NLR, and SLFN11 were significant predictors of patient survival according to Kaplan–Meier analysis. The combination of inflammatory parameters and SLFN11 with a cutoff value above the 75th percentile of the predicted probability was associated with significantly lower overall survival in SCLC patients (average survival of 3.6 months vs. 4.8 months). Conclusion: The combination of inflammatory markers and the levels of two specific proteins (sPD-L1, SLFN11) could potentially serve as a non-invasive biomarker for predicting responses to DNA-damaging therapeutic agents in SCLC.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study",
volume = "12",
number = "5",
doi = "10.3390/biomedicines12050976"
}

Simić, I., Guzonjić, A., Kotur-Stevuljević, J., Ćeriman Krstić, V., Samardžić, N., Savić Vujović, K.,& Jovanović, D.. (2024). Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines
MDPI., 12(5).
https://doi.org/10.3390/biomedicines12050976

Simić I, Guzonjić A, Kotur-Stevuljević J, Ćeriman Krstić V, Samardžić N, Savić Vujović K, Jovanović D. Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines. 2024;12(5).
doi:10.3390/biomedicines12050976 .

Simić, Ivana, Guzonjić, Azra, Kotur-Stevuljević, Jelena, Ćeriman Krstić, Vesna, Samardžić, Natalija, Savić Vujović, Katarina, Jovanović, Dragana, "Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study" in Biomedicines, 12, no. 5 (2024),
https://doi.org/10.3390/biomedicines12050976 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Stefanović, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5622
AB  - Childhood obesity with its growing prevalence worldwide presents one of the most important health challenges nowadays. Multiple mechanisms are involved in the development of this condition, as well as in its associations with various cardiometabolic complications, such as insulin resistance, diabetes, metabolic dysfunction-associated steatotic liver disease and cardiovascular diseases. Recent findings suggest that childhood obesity and associated dyslipidemia at least partly originate from epigenetic modifications that take place in the earliest periods of life, namely prenatal and perinatal periods. Hence, alterations of maternal metabolism could be fundamentally responsible for fetal and neonatal metabolic programming and consequently, for metabolic health of offspring in later life. In this paper, we will review recent findings on the associations among intrauterine and early postnatal exposure to undesirable modulators of metabolism, development of childhood obesity and later cardiometabolic complications. Special attention will be given to maternal dyslipidemia as a driven force for undesirable epigenetic modulations in offspring. In addition, newly proposed lipid biomarkers of increased cardiometabolic risk in obese children and adolescents will be analyzed, with respect to their predictive potential and clinical applicability.
PB  - W.B. Saunders
T2  - Metabolism: Clinical and Experimental
T1  - Obesity and dyslipidemia in early life: Impact on cardiometabolic risk
VL  - 156
DO  - 10.1016/j.metabol.2024.155919
ER  - 

@article{
author = "Zeljković, Aleksandra and Vekić, Jelena and Stefanović, Aleksandra",
year = "2024",
abstract = "Childhood obesity with its growing prevalence worldwide presents one of the most important health challenges nowadays. Multiple mechanisms are involved in the development of this condition, as well as in its associations with various cardiometabolic complications, such as insulin resistance, diabetes, metabolic dysfunction-associated steatotic liver disease and cardiovascular diseases. Recent findings suggest that childhood obesity and associated dyslipidemia at least partly originate from epigenetic modifications that take place in the earliest periods of life, namely prenatal and perinatal periods. Hence, alterations of maternal metabolism could be fundamentally responsible for fetal and neonatal metabolic programming and consequently, for metabolic health of offspring in later life. In this paper, we will review recent findings on the associations among intrauterine and early postnatal exposure to undesirable modulators of metabolism, development of childhood obesity and later cardiometabolic complications. Special attention will be given to maternal dyslipidemia as a driven force for undesirable epigenetic modulations in offspring. In addition, newly proposed lipid biomarkers of increased cardiometabolic risk in obese children and adolescents will be analyzed, with respect to their predictive potential and clinical applicability.",
publisher = "W.B. Saunders",
journal = "Metabolism: Clinical and Experimental",
title = "Obesity and dyslipidemia in early life: Impact on cardiometabolic risk",
volume = "156",
doi = "10.1016/j.metabol.2024.155919"
}

Zeljković, A., Vekić, J.,& Stefanović, A.. (2024). Obesity and dyslipidemia in early life: Impact on cardiometabolic risk. in Metabolism: Clinical and Experimental
W.B. Saunders., 156.
https://doi.org/10.1016/j.metabol.2024.155919

Zeljković A, Vekić J, Stefanović A. Obesity and dyslipidemia in early life: Impact on cardiometabolic risk. in Metabolism: Clinical and Experimental. 2024;156.
doi:10.1016/j.metabol.2024.155919 .

Zeljković, Aleksandra, Vekić, Jelena, Stefanović, Aleksandra, "Obesity and dyslipidemia in early life: Impact on cardiometabolic risk" in Metabolism: Clinical and Experimental, 156 (2024),
https://doi.org/10.1016/j.metabol.2024.155919 . .

TY  - JOUR
AU  - Pejčić, Zorica
AU  - Topić Vučenović, Valentina
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5649
AB  - Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.
PB  - MDPI
T2  - Pharmaceutics
T1  - Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050685
ER  - 

@article{
author = "Pejčić, Zorica and Topić Vučenović, Valentina and Miljković, Branislava and Vučićević, Katarina",
year = "2024",
abstract = "Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050685"
}

Pejčić, Z., Topić Vučenović, V., Miljković, B.,& Vučićević, K.. (2024). Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050685

Pejčić Z, Topić Vučenović V, Miljković B, Vučićević K. Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050685 .

Pejčić, Zorica, Topić Vučenović, Valentina, Miljković, Branislava, Vučićević, Katarina, "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050685 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Munjas, Jelena
AU  - Kovačević, Smiljka
AU  - Guzonjić, Azra
AU  - Mitrović, Jadranka
AU  - Saso, Luciano
AU  - Đuričić, Ivana
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5654
AB  - Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Childhood obesity accelerates biological ageing: is oxidative stress a link?
DO  - 10.1017/S0007114524000898
ER  - 

@article{
author = "Subošić, Branko and Zdravković, Vera and Ješić, Maja and Munjas, Jelena and Kovačević, Smiljka and Guzonjić, Azra and Mitrović, Jadranka and Saso, Luciano and Đuričić, Ivana and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Childhood obesity accelerates biological ageing: is oxidative stress a link?",
doi = "10.1017/S0007114524000898"
}

Subošić, B., Zdravković, V., Ješić, M., Munjas, J., Kovačević, S., Guzonjić, A., Mitrović, J., Saso, L., Đuričić, I.,& Kotur-Stevuljević, J.. (2024). Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition
Cambridge University Press..
https://doi.org/10.1017/S0007114524000898

Subošić B, Zdravković V, Ješić M, Munjas J, Kovačević S, Guzonjić A, Mitrović J, Saso L, Đuričić I, Kotur-Stevuljević J. Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition. 2024;.
doi:10.1017/S0007114524000898 .

Subošić, Branko, Zdravković, Vera, Ješić, Maja, Munjas, Jelena, Kovačević, Smiljka, Guzonjić, Azra, Mitrović, Jadranka, Saso, Luciano, Đuričić, Ivana, Kotur-Stevuljević, Jelena, "Childhood obesity accelerates biological ageing: is oxidative stress a link?" in British Journal of Nutrition (2024),
https://doi.org/10.1017/S0007114524000898 . .

TY  - JOUR
AU  - Tomić, Tanja
AU  - Henman, Martin
AU  - Tadić, Ivana
AU  - Antić-Stanković, Jelena
AU  - Santric Milicevic, Milena
AU  - Bukumirić, Zoran
AU  - Lakić, Dragana
AU  - Odalović, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5653
AB  - Introduction: The coronavirus disease 2019 (COVID-19) pandemic affected antibiotic usage worldwide. However, there is limited data from Serbia. Dispensing of oral antibiotics in Serbian pharmacies was analyzed to calculate monthly and yearly changes between 2018-2021, and to explore immediate and long-term effects of COVID-19 on antibiotic dispensing during this period. Methodology: The number of antibiotic packages dispensed from pharmacies during the study period was analyzed with a Chi-square test to assess the average change in annual dispensing, and an interrupted time-series analysis was used to evaluate the impact of the pandemic on antibiotic dispensing. The data from 2018-2021 were retrieved from the database of a large community pharmacy chain in Serbia. Results: The average number of antibiotic packages dispensed per day and per pharmacy was higher in 2021 compared to 2018 by one package. However, the dispensing of macrolides increased significantly; 17.7% (2018) vs. 22.5% (2021) (p < 0.05). In general, an increase in antibiotic dispensing was detected during COVID-19 for total antibiotics (16.4%), Watch antibiotics (44.8%), third-generation cephalosporins (80.4%), macrolides (45.5%) and azithromycin (83.7%). However, the immediate effect of COVID-19 was a decrease in the dispensing of Watch antibiotics, penicillin, and third-generation cephalosporins (p < 0.05); and a notable long-term COVID-19 effect was an increase in the dispensing of azithromycin (p < 0.05). Conclusions: In spite of a relatively stable trend of total antibiotic dispensing before and during COVID-19 pandemic, the use of Watch antibiotics, third-generation cephalosporins, and macrolides (particularly azithromycin) showed an increasing trend in dispensing that should be optimized.
PB  - Journal of Infection in Developing Countries
T2  - Journal of Infection in Developing Countries
T1  - Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics
VL  - 18
IS  - 4
SP  - 504
EP  - 512
DO  - 10.3855/jidc.19255
ER  - 

@article{
author = "Tomić, Tanja and Henman, Martin and Tadić, Ivana and Antić-Stanković, Jelena and Santric Milicevic, Milena and Bukumirić, Zoran and Lakić, Dragana and Odalović, Marina",
year = "2024",
abstract = "Introduction: The coronavirus disease 2019 (COVID-19) pandemic affected antibiotic usage worldwide. However, there is limited data from Serbia. Dispensing of oral antibiotics in Serbian pharmacies was analyzed to calculate monthly and yearly changes between 2018-2021, and to explore immediate and long-term effects of COVID-19 on antibiotic dispensing during this period. Methodology: The number of antibiotic packages dispensed from pharmacies during the study period was analyzed with a Chi-square test to assess the average change in annual dispensing, and an interrupted time-series analysis was used to evaluate the impact of the pandemic on antibiotic dispensing. The data from 2018-2021 were retrieved from the database of a large community pharmacy chain in Serbia. Results: The average number of antibiotic packages dispensed per day and per pharmacy was higher in 2021 compared to 2018 by one package. However, the dispensing of macrolides increased significantly; 17.7% (2018) vs. 22.5% (2021) (p < 0.05). In general, an increase in antibiotic dispensing was detected during COVID-19 for total antibiotics (16.4%), Watch antibiotics (44.8%), third-generation cephalosporins (80.4%), macrolides (45.5%) and azithromycin (83.7%). However, the immediate effect of COVID-19 was a decrease in the dispensing of Watch antibiotics, penicillin, and third-generation cephalosporins (p < 0.05); and a notable long-term COVID-19 effect was an increase in the dispensing of azithromycin (p < 0.05). Conclusions: In spite of a relatively stable trend of total antibiotic dispensing before and during COVID-19 pandemic, the use of Watch antibiotics, third-generation cephalosporins, and macrolides (particularly azithromycin) showed an increasing trend in dispensing that should be optimized.",
publisher = "Journal of Infection in Developing Countries",
journal = "Journal of Infection in Developing Countries",
title = "Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics",
volume = "18",
number = "4",
pages = "504-512",
doi = "10.3855/jidc.19255"
}

Tomić, T., Henman, M., Tadić, I., Antić-Stanković, J., Santric Milicevic, M., Bukumirić, Z., Lakić, D.,& Odalović, M.. (2024). Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics. in Journal of Infection in Developing Countries
Journal of Infection in Developing Countries., 18(4), 504-512.
https://doi.org/10.3855/jidc.19255

Tomić T, Henman M, Tadić I, Antić-Stanković J, Santric Milicevic M, Bukumirić Z, Lakić D, Odalović M. Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics. in Journal of Infection in Developing Countries. 2024;18(4):504-512.
doi:10.3855/jidc.19255 .

Tomić, Tanja, Henman, Martin, Tadić, Ivana, Antić-Stanković, Jelena, Santric Milicevic, Milena, Bukumirić, Zoran, Lakić, Dragana, Odalović, Marina, "Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics" in Journal of Infection in Developing Countries, 18, no. 4 (2024):504-512,
https://doi.org/10.3855/jidc.19255 . .