Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography
Апстракт
A thin-layer chromatographic method for simultaneous determination of moxonidine and its four impurities was developed and validated. Separation of the examined compounds was performed on chromatographic plates precoated with silica gel 60F(254) and using methanol-toluene-dichloroethane-ammonia 2:3:3:0.1 (v/v/v/v) as mobile phase. Ascending development mode was performed in the twin-trough chromatographic chamber, which was presaturated with mobile phase vapors for 15min. The developed chromatographic plates were dried in air and densitometrically scanned at the wavelengths of 260 and 280nm. Regression coefficients (r >= 0.998), recovery (90.10-107.63%), limit of quantification of impurities (20ng band(-1) equivalent to the 0.12% impurity level), and robustness were validated and found satisfactory. The developed method is well suited for quantitative analysis and purity control of moxonidine in its dosage forms.
Кључне речи:
pharmaceuticals / moxonidine / method validation / quantitation / impurities / TLCИзвор:
Journal of Liquid Chromatography & Related Technologies, 2015, 38, 11, 1121-1125Издавач:
- Taylor & Francis Inc, Philadelphia
Финансирање / пројекти:
- Синтеза, квантитативни однос између структуре и дејства, физичко-хемијска карактеризација и анализа фармаколошки активних супстанци (RS-MESTD-Basic Research (BR or ON)-172033)
Напомена:
- Peer-reviewed manuscript: http://farfar.pharmacy.bg.ac.rs/handle/123456789/3439
DOI: 10.1080/10826076.2015.1028291
ISSN: 1082-6076
WoS: 000354045900006
Scopus: 2-s2.0-84929231814
Институција/група
PharmacyTY - JOUR AU - Filipić, Slavica AU - Shenger, Musbah Salem Mohamed AU - Nikolić, Katarina AU - Agbaba, Danica PY - 2015 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2391 AB - A thin-layer chromatographic method for simultaneous determination of moxonidine and its four impurities was developed and validated. Separation of the examined compounds was performed on chromatographic plates precoated with silica gel 60F(254) and using methanol-toluene-dichloroethane-ammonia 2:3:3:0.1 (v/v/v/v) as mobile phase. Ascending development mode was performed in the twin-trough chromatographic chamber, which was presaturated with mobile phase vapors for 15min. The developed chromatographic plates were dried in air and densitometrically scanned at the wavelengths of 260 and 280nm. Regression coefficients (r >= 0.998), recovery (90.10-107.63%), limit of quantification of impurities (20ng band(-1) equivalent to the 0.12% impurity level), and robustness were validated and found satisfactory. The developed method is well suited for quantitative analysis and purity control of moxonidine in its dosage forms. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography & Related Technologies T1 - Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography VL - 38 IS - 11 SP - 1121 EP - 1125 DO - 10.1080/10826076.2015.1028291 ER -
@article{ author = "Filipić, Slavica and Shenger, Musbah Salem Mohamed and Nikolić, Katarina and Agbaba, Danica", year = "2015", abstract = "A thin-layer chromatographic method for simultaneous determination of moxonidine and its four impurities was developed and validated. Separation of the examined compounds was performed on chromatographic plates precoated with silica gel 60F(254) and using methanol-toluene-dichloroethane-ammonia 2:3:3:0.1 (v/v/v/v) as mobile phase. Ascending development mode was performed in the twin-trough chromatographic chamber, which was presaturated with mobile phase vapors for 15min. The developed chromatographic plates were dried in air and densitometrically scanned at the wavelengths of 260 and 280nm. Regression coefficients (r >= 0.998), recovery (90.10-107.63%), limit of quantification of impurities (20ng band(-1) equivalent to the 0.12% impurity level), and robustness were validated and found satisfactory. The developed method is well suited for quantitative analysis and purity control of moxonidine in its dosage forms.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography & Related Technologies", title = "Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography", volume = "38", number = "11", pages = "1121-1125", doi = "10.1080/10826076.2015.1028291" }
Filipić, S., Shenger, M. S. M., Nikolić, K.,& Agbaba, D.. (2015). Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies Taylor & Francis Inc, Philadelphia., 38(11), 1121-1125. https://doi.org/10.1080/10826076.2015.1028291
Filipić S, Shenger MSM, Nikolić K, Agbaba D. Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies. 2015;38(11):1121-1125. doi:10.1080/10826076.2015.1028291 .
Filipić, Slavica, Shenger, Musbah Salem Mohamed, Nikolić, Katarina, Agbaba, Danica, "Determination of Moxonidine and Its Impurities by Thin-Layer Chromatography" in Journal of Liquid Chromatography & Related Technologies, 38, no. 11 (2015):1121-1125, https://doi.org/10.1080/10826076.2015.1028291 . .