Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties
Abstract
A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H hirsutum, H maculatum, and H richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3,II8-biapigenin, orientin, 2"-O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by... Fe2+. The most potent were extracts of H acutum and H maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.
Keywords:
Hypericum species / Chemical analysis / LC/MS / HPLC/DAD / Antioxidant activitySource:
Natural Product Communications, 2017, 12, 11, 1805-1811Publisher:
- Sage Publications Inc, Thousand Oaks
Collections
Institution/Community
PharmacyTY - JOUR AU - Zdunić, Gordana AU - Gođevac, Dejan AU - Šavikin, Katarina AU - Petrović, Silvana PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2859 AB - A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H hirsutum, H maculatum, and H richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3,II8-biapigenin, orientin, 2"-O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H acutum and H maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds. PB - Sage Publications Inc, Thousand Oaks T2 - Natural Product Communications T1 - Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties VL - 12 IS - 11 SP - 1805 EP - 1811 UR - https://hdl.handle.net/21.15107/rcub_farfar_2859 ER -
@article{ author = "Zdunić, Gordana and Gođevac, Dejan and Šavikin, Katarina and Petrović, Silvana", year = "2017", abstract = "A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H hirsutum, H maculatum, and H richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3,II8-biapigenin, orientin, 2"-O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H acutum and H maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.", publisher = "Sage Publications Inc, Thousand Oaks", journal = "Natural Product Communications", title = "Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties", volume = "12", number = "11", pages = "1805-1811", url = "https://hdl.handle.net/21.15107/rcub_farfar_2859" }
Zdunić, G., Gođevac, D., Šavikin, K.,& Petrović, S.. (2017). Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. in Natural Product Communications Sage Publications Inc, Thousand Oaks., 12(11), 1805-1811. https://hdl.handle.net/21.15107/rcub_farfar_2859
Zdunić G, Gođevac D, Šavikin K, Petrović S. Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. in Natural Product Communications. 2017;12(11):1805-1811. https://hdl.handle.net/21.15107/rcub_farfar_2859 .
Zdunić, Gordana, Gođevac, Dejan, Šavikin, Katarina, Petrović, Silvana, "Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties" in Natural Product Communications, 12, no. 11 (2017):1805-1811, https://hdl.handle.net/21.15107/rcub_farfar_2859 .