TY  - CONF
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Rajab Elferjane, Rajab Elferjane
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5261
AB  - Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.
PB  - University of East Sarajevo, Faculty of technology
C3  - VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
T1  - Maceration and heat-assisted extraction of polyphenols from Aloe vera
SP  - 136
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5261
ER  - 

@conference{
author = "Čutović, Natalija and Jovanović, Aleksandra and Rajab Elferjane, Rajab Elferjane and Milutinović, Violeta and Petrović, Predrag and Marinković, Aleksandar and Bugarski, Branko",
year = "2023",
abstract = "Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.",
publisher = "University of East Sarajevo, Faculty of technology",
journal = "VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings",
title = "Maceration and heat-assisted extraction of polyphenols from Aloe vera",
pages = "136-143",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5261"
}

Čutović, N., Jovanović, A., Rajab Elferjane, R. E., Milutinović, V., Petrović, P., Marinković, A.,& Bugarski, B.. (2023). Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
University of East Sarajevo, Faculty of technology., 136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261

Čutović N, Jovanović A, Rajab Elferjane RE, Milutinović V, Petrović P, Marinković A, Bugarski B. Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings. 2023;:136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

Čutović, Natalija, Jovanović, Aleksandra, Rajab Elferjane, Rajab Elferjane, Milutinović, Violeta, Petrović, Predrag, Marinković, Aleksandar, Bugarski, Branko, "Maceration and heat-assisted extraction of polyphenols from Aloe vera" in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings (2023):136-143,
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Petković, Miloš
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5180
AB  - The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites
VL  - 20
IS  - 11
SP  - e202301044
DO  - 10.1002/cbdv.202301044
ER  - 

@article{
author = "Milutinović, Violeta and Petrović, Predrag and Petković, Miloš and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites",
volume = "20",
number = "11",
pages = "e202301044",
doi = "10.1002/cbdv.202301044"
}

Milutinović, V., Petrović, P., Petković, M., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity
John Wiley and Sons Inc., 20(11), e202301044.
https://doi.org/10.1002/cbdv.202301044

Milutinović V, Petrović P, Petković M, Klaus A, Ušjak L, Niketić M, Petrović S. Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity. 2023;20(11):e202301044.
doi:10.1002/cbdv.202301044 .

Milutinović, Violeta, Petrović, Predrag, Petković, Miloš, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites" in Chemistry and Biodiversity, 20, no. 11 (2023):e202301044,
https://doi.org/10.1002/cbdv.202301044 . .

TY  - CONF
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Bigović, Dubravka
AU  - Petrović, Predrag
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5120
AB  - Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
T1  - Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5120
ER  - 

@conference{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Bigović, Dubravka and Petrović, Predrag and Pljevljakušić, Dejan and Šavikin, Katarina and Filipić, Brankica",
year = "2022",
abstract = "Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts",
title = "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5120"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Bigović, D., Petrović, P., Pljevljakušić, D., Šavikin, K.,& Filipić, B.. (2022). Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
Belgrade : Institute of Technical Sciences of SASA., 14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120

Kuzmanović Nedeljković S, Ćujić Nikolić N, Bigović D, Petrović P, Pljevljakušić D, Šavikin K, Filipić B. Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts. 2022;:14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Bigović, Dubravka, Petrović, Predrag, Pljevljakušić, Dejan, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin" in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts (2022):14-14,
https://hdl.handle.net/21.15107/rcub_farfar_5120 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5110
AB  - The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5110
ER  - 

@conference{
author = "Milutinović, Violeta and Petrović, Predrag and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5110"
}

Milutinović, V., Petrović, P., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2019). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110

Milutinović V, Petrović P, Klaus A, Ušjak L, Niketić M, Petrović S. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

Milutinović, Violeta, Petrović, Predrag, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, J.
AU  - Klaus, A.
AU  - Carević, Momir
AU  - Petković, Miloš
AU  - Vuković, N.
AU  - Cvetković, A.
AU  - Žižak, Željko
AU  - Bugarski, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3357
AB  - Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.
PB  - Elsevier Science BV, Amsterdam
T2  - Southern Medical Journal
T1  - From mycelium to spores: A whole circle of biological potency of mosaic puffball
VL  - 123
SP  - 152
EP  - 160
DO  - 10.1016/j.sajb.2019.03.016
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, J. and Klaus, A. and Carević, Momir and Petković, Miloš and Vuković, N. and Cvetković, A. and Žižak, Željko and Bugarski, Branko",
year = "2019",
abstract = "Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Southern Medical Journal",
title = "From mycelium to spores: A whole circle of biological potency of mosaic puffball",
volume = "123",
pages = "152-160",
doi = "10.1016/j.sajb.2019.03.016"
}

Petrović, P., Vunduk, J., Klaus, A., Carević, M., Petković, M., Vuković, N., Cvetković, A., Žižak, Ž.,& Bugarski, B.. (2019). From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal
Elsevier Science BV, Amsterdam., 123, 152-160.
https://doi.org/10.1016/j.sajb.2019.03.016

Petrović P, Vunduk J, Klaus A, Carević M, Petković M, Vuković N, Cvetković A, Žižak Ž, Bugarski B. From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal. 2019;123:152-160.
doi:10.1016/j.sajb.2019.03.016 .

Petrović, Predrag, Vunduk, J., Klaus, A., Carević, Momir, Petković, Miloš, Vuković, N., Cvetković, A., Žižak, Željko, Bugarski, Branko, "From mycelium to spores: A whole circle of biological potency of mosaic puffball" in Southern Medical Journal, 123 (2019):152-160,
https://doi.org/10.1016/j.sajb.2019.03.016 . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Jančić, Ivan
AU  - Janković-Castvan, Ivona
AU  - Petrović, Predrag
AU  - Milenković, Marina
AU  - Obradović, Bojana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3308
AB  - Composites based on Zn-alginate hydrogels in the form of beads were produced by extrusion of a suspension containing 0.5 % w/w of alginate and 20 % w/w of activated charcoal (AC) with the intent to simultaneously release two active agents, Zn2+ and AC particles, in a physiological-like environment. The obtained composite beads were analyzed by FE-SEM and characterized regarding textural parameters, as well as Zn2+ and AC release kinetics in the physiological saline solution. Zn(2+)ions were quickly released reaching the equilibrium concentration within the first hour in the contrary to the release of AC particles, which was described by internal diffusion with the apparent diffusion coefficient of approximately 10(-13) m(2) s(-1). Potential functionality of the obtained beads was evaluated regarding antibacterial activity in suspensions of the standard bacterial strain Escherichia coli 25922. The observed strong bactericidal effects were related to the quick release of Zn2+ that was not affected by AC. Thus, taking into account results of this study, as well as high sorption capacity of alginate hydrogel, efficiency of AC to adsorb malodor and tissue degradation products and positive effects of Zn2+ on wound healing, the obtained composites have shown promising potentials for applications as multifunctional wound dressings.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings
VL  - 73
IS  - 1
SP  - 37
EP  - 46
DO  - 10.2298/HEMIND180629003O
ER  - 

@article{
author = "Osmokrović, Andrea and Jančić, Ivan and Janković-Castvan, Ivona and Petrović, Predrag and Milenković, Marina and Obradović, Bojana",
year = "2019",
abstract = "Composites based on Zn-alginate hydrogels in the form of beads were produced by extrusion of a suspension containing 0.5 % w/w of alginate and 20 % w/w of activated charcoal (AC) with the intent to simultaneously release two active agents, Zn2+ and AC particles, in a physiological-like environment. The obtained composite beads were analyzed by FE-SEM and characterized regarding textural parameters, as well as Zn2+ and AC release kinetics in the physiological saline solution. Zn(2+)ions were quickly released reaching the equilibrium concentration within the first hour in the contrary to the release of AC particles, which was described by internal diffusion with the apparent diffusion coefficient of approximately 10(-13) m(2) s(-1). Potential functionality of the obtained beads was evaluated regarding antibacterial activity in suspensions of the standard bacterial strain Escherichia coli 25922. The observed strong bactericidal effects were related to the quick release of Zn2+ that was not affected by AC. Thus, taking into account results of this study, as well as high sorption capacity of alginate hydrogel, efficiency of AC to adsorb malodor and tissue degradation products and positive effects of Zn2+ on wound healing, the obtained composites have shown promising potentials for applications as multifunctional wound dressings.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings",
volume = "73",
number = "1",
pages = "37-46",
doi = "10.2298/HEMIND180629003O"
}

Osmokrović, A., Jančić, I., Janković-Castvan, I., Petrović, P., Milenković, M.,& Obradović, B.. (2019). Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 73(1), 37-46.
https://doi.org/10.2298/HEMIND180629003O

Osmokrović A, Jančić I, Janković-Castvan I, Petrović P, Milenković M, Obradović B. Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings. in Hemijska industrija. 2019;73(1):37-46.
doi:10.2298/HEMIND180629003O .

Osmokrović, Andrea, Jančić, Ivan, Janković-Castvan, Ivona, Petrović, Predrag, Milenković, Marina, Obradović, Bojana, "Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings" in Hemijska industrija, 73, no. 1 (2019):37-46,
https://doi.org/10.2298/HEMIND180629003O . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Jančić, Ivan
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Milenković, Marina
AU  - Obradović, Bojana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3240
AB  - New composites based on Ca-alginate hydrogels were produced that release activated charcoal (AC) particles with adsorbed povidone iodine (PVP-I) as a model antimicrobial substance in a physiological-like environment. Composite beads with different alginate (0.5-1.5% w/w) and AC (1-20% w/w) concentrations were analyzed by FE-SEM and characterized regarding textural parameters, swelling, and AC release kinetics. PVP-I was easily adsorbed onto AC particles within the optimized beads (0.5% w/w alginate, 20% w/w AC) as indicated by UV-vis spectroscopy, EDX and FT-IR analyses. The obtained beads have shown strong bactericidal effects against two standard bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa) and clinical multi-resistant wound isolates (MRSA, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Proteus mirabilis) and, at the same time, exhibited negligible PVP-I desorption in physiological saline solution. Thus, the obtained composites could provide utilization of potent antiseptics such as iodine, in wound dressings, without the concern of systemic absorption.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent
VL  - 196
SP  - 279
EP  - 288
DO  - 10.1016/j.carbpol.2018.05.045
ER  - 

@article{
author = "Osmokrović, Andrea and Jančić, Ivan and Vunduk, Jovana and Petrović, Predrag and Milenković, Marina and Obradović, Bojana",
year = "2018",
abstract = "New composites based on Ca-alginate hydrogels were produced that release activated charcoal (AC) particles with adsorbed povidone iodine (PVP-I) as a model antimicrobial substance in a physiological-like environment. Composite beads with different alginate (0.5-1.5% w/w) and AC (1-20% w/w) concentrations were analyzed by FE-SEM and characterized regarding textural parameters, swelling, and AC release kinetics. PVP-I was easily adsorbed onto AC particles within the optimized beads (0.5% w/w alginate, 20% w/w AC) as indicated by UV-vis spectroscopy, EDX and FT-IR analyses. The obtained beads have shown strong bactericidal effects against two standard bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa) and clinical multi-resistant wound isolates (MRSA, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Proteus mirabilis) and, at the same time, exhibited negligible PVP-I desorption in physiological saline solution. Thus, the obtained composites could provide utilization of potent antiseptics such as iodine, in wound dressings, without the concern of systemic absorption.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent",
volume = "196",
pages = "279-288",
doi = "10.1016/j.carbpol.2018.05.045"
}

Osmokrović, A., Jančić, I., Vunduk, J., Petrović, P., Milenković, M.,& Obradović, B.. (2018). Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 196, 279-288.
https://doi.org/10.1016/j.carbpol.2018.05.045

Osmokrović A, Jančić I, Vunduk J, Petrović P, Milenković M, Obradović B. Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent. in Carbohydrate Polymers. 2018;196:279-288.
doi:10.1016/j.carbpol.2018.05.045 .

Osmokrović, Andrea, Jančić, Ivan, Vunduk, Jovana, Petrović, Predrag, Milenković, Marina, Obradović, Bojana, "Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent" in Carbohydrate Polymers, 196 (2018):279-288,
https://doi.org/10.1016/j.carbpol.2018.05.045 . .