TY  - JOUR
AU  - Radulović, Valentina
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
AU  - Rajić, K.K
AU  - Jovanović, M
AU  - Marjanović, I
AU  - Stojković, M
AU  - Agbaba, Danica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3286
AB  - A novel voltammetric method was developed for brimonidine (BRIM) determination in deproteinized aqueous humor, simplifying preparation of biological samples for analysis for stability studies. The differential pulse voltammetric (DPV) method using boron doped diamond electrode (BDDE), based on characteristic oxidation peaks, was proposed and successfully applied. The linearity range was within 5.0 × 10−6 to 5.0 × 10−5 M of brimonidine, and limit of detection and limit of quantitation were 1.94 × 10−6 M and 6.46 × 10−6 M, respectively. Intra-day and inter-day precision and accuracy were evaluated and all results were in accordance with validation ICH guidelines. The best short-term stability study results were obtained for a concentration level of 3.0 × 10−5 M expressed by deviation of + 1.86% between initial and post storage concentrations. A long-term stability study was performed for two concentrations of 3.0 × 10−5 M and 5.0 × 10−5 M and resulted in deviations of + 1.63% and + 3.56%, respectively. A freeze and thaw stability study indicated that samples might be frozen only once. The enhancement of DPV/BDDE method sensitivity gained by modification, for the analysis of immeasurable BRIM quantities in native, untreated aqueous humor, was reached for quantities of 6 or 12 nmol/0.1 mL aqueous humor with acceptable accuracy (up to + 7.5%). The nature of the process—the irreversible one electron oxidation voltammetric peak of BRIM—limited the sensitivity. Only electrochemical pre-treatment of the BDD electrode before each measurement significantly speeded up the whole procedure. The advantages of the proposed method are simplicity, short-time performance, and good specificity/selectivity, as well as satisfactory accuracy, and no chemical modification of BDDE was necessary.
PB  - Springer Verlag
T2  - Analytical and Bioanalytical Chemistry
T1  - The evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samples
DO  - 10.1007/s00216-019-01955-3
ER  - 

@article{
author = "Radulović, Valentina and Aleksić, Mara and Kapetanović, Vera and Rajić, K.K and Jovanović, M and Marjanović, I and Stojković, M and Agbaba, Danica",
year = "2019",
abstract = "A novel voltammetric method was developed for brimonidine (BRIM) determination in deproteinized aqueous humor, simplifying preparation of biological samples for analysis for stability studies. The differential pulse voltammetric (DPV) method using boron doped diamond electrode (BDDE), based on characteristic oxidation peaks, was proposed and successfully applied. The linearity range was within 5.0 × 10−6 to 5.0 × 10−5 M of brimonidine, and limit of detection and limit of quantitation were 1.94 × 10−6 M and 6.46 × 10−6 M, respectively. Intra-day and inter-day precision and accuracy were evaluated and all results were in accordance with validation ICH guidelines. The best short-term stability study results were obtained for a concentration level of 3.0 × 10−5 M expressed by deviation of + 1.86% between initial and post storage concentrations. A long-term stability study was performed for two concentrations of 3.0 × 10−5 M and 5.0 × 10−5 M and resulted in deviations of + 1.63% and + 3.56%, respectively. A freeze and thaw stability study indicated that samples might be frozen only once. The enhancement of DPV/BDDE method sensitivity gained by modification, for the analysis of immeasurable BRIM quantities in native, untreated aqueous humor, was reached for quantities of 6 or 12 nmol/0.1 mL aqueous humor with acceptable accuracy (up to + 7.5%). The nature of the process—the irreversible one electron oxidation voltammetric peak of BRIM—limited the sensitivity. Only electrochemical pre-treatment of the BDD electrode before each measurement significantly speeded up the whole procedure. The advantages of the proposed method are simplicity, short-time performance, and good specificity/selectivity, as well as satisfactory accuracy, and no chemical modification of BDDE was necessary.",
publisher = "Springer Verlag",
journal = "Analytical and Bioanalytical Chemistry",
title = "The evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samples",
doi = "10.1007/s00216-019-01955-3"
}

Radulović, V., Aleksić, M., Kapetanović, V., Rajić, K.K, Jovanović, M., Marjanović, I., Stojković, M.,& Agbaba, D.. (2019). The evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samples. in Analytical and Bioanalytical Chemistry
Springer Verlag..
https://doi.org/10.1007/s00216-019-01955-3

Radulović V, Aleksić M, Kapetanović V, Rajić K, Jovanović M, Marjanović I, Stojković M, Agbaba D. The evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samples. in Analytical and Bioanalytical Chemistry. 2019;.
doi:10.1007/s00216-019-01955-3 .

Radulović, Valentina, Aleksić, Mara, Kapetanović, Vera, Rajić, K.K, Jovanović, M, Marjanović, I, Stojković, M, Agbaba, Danica, "The evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samples" in Analytical and Bioanalytical Chemistry (2019),
https://doi.org/10.1007/s00216-019-01955-3 . .

TY  - CONF
AU  - Pantić, Jelena
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
AU  - Ružić, Dušan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4923
AB  - Electrochemical behavior of sulfaquinoxaline (SQN) was investigated using cyclic (CV) and differential pulse voltammetry (DPV) at glassy carbon electrode (GCE). CV was applied to investigate the effect of the supporting electrolyte pH on the SQN electrochemical behavior. In the pH range 2.0 – 10.0, the best current response was obtained at pH 7.0 in phosphate buffer. Results indicated that SQN is oxidized in irreversible process. DPV method was optimized, and the linear dependence of peak current vs. SQN concentration was obtained in the range 3×10-6 ‒ 5×10-5 molL-1, with limits of detection and quantification of 1.53×10-6 molL-1 and 5.10×10-6 molL-1, respectively. DPV was successively applied for SQN determination in veterinary medicine NEOCOCCYN WSP, which contains amprolium hydrochloride, as active component too.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia
T1  - Electrochemical behavior and determination od sulfaquinoxaline at glassy carbon electrode
VL  - II
SP  - 829
EP  - 832
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4923
ER  - 

@conference{
author = "Pantić, Jelena and Aleksić, Mara and Kapetanović, Vera and Ružić, Dušan",
year = "2016",
abstract = "Electrochemical behavior of sulfaquinoxaline (SQN) was investigated using cyclic (CV) and differential pulse voltammetry (DPV) at glassy carbon electrode (GCE). CV was applied to investigate the effect of the supporting electrolyte pH on the SQN electrochemical behavior. In the pH range 2.0 – 10.0, the best current response was obtained at pH 7.0 in phosphate buffer. Results indicated that SQN is oxidized in irreversible process. DPV method was optimized, and the linear dependence of peak current vs. SQN concentration was obtained in the range 3×10-6 ‒ 5×10-5 molL-1, with limits of detection and quantification of 1.53×10-6 molL-1 and 5.10×10-6 molL-1, respectively. DPV was successively applied for SQN determination in veterinary medicine NEOCOCCYN WSP, which contains amprolium hydrochloride, as active component too.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia",
title = "Electrochemical behavior and determination od sulfaquinoxaline at glassy carbon electrode",
volume = "II",
pages = "829-832",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4923"
}

Pantić, J., Aleksić, M., Kapetanović, V.,& Ružić, D.. (2016). Electrochemical behavior and determination od sulfaquinoxaline at glassy carbon electrode. in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia
Society of Physical Chemists of Serbia., II, 829-832.
https://hdl.handle.net/21.15107/rcub_farfar_4923

Pantić J, Aleksić M, Kapetanović V, Ružić D. Electrochemical behavior and determination od sulfaquinoxaline at glassy carbon electrode. in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia. 2016;II:829-832.
https://hdl.handle.net/21.15107/rcub_farfar_4923 .

Pantić, Jelena, Aleksić, Mara, Kapetanović, Vera, Ružić, Dušan, "Electrochemical behavior and determination od sulfaquinoxaline at glassy carbon electrode" in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia, II (2016):829-832,
https://hdl.handle.net/21.15107/rcub_farfar_4923 .

TY  - JOUR
AU  - Nikolić, Katarina
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
AU  - Agbaba, Danica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2300
AB  - The adsorption and electroreduction behavior of cefpodoxime proxetil, cefotaxime, desacetylcefotaxime, cefetamet, ceftriaxone, ceftazidime, and cefuroxime axetil at a mercury electrode surface were studied using cyclic (CV), differential pulse (DPV) and adsorptive stripping differential pulse (AdSDPV) voltammetry. The quantitative structure property relationship (QSPR) study of the seven cephalosporins adsorption at the mercury electrode was based on density functional theory DFT-B3LYP/6-31G(d,p) calculations of molecular orbitals, partial charges and electron densities of the analytes. The DFT-parameters and QSPR model explain well the process of adsorption of the examined cephalosporins. The QSPR study defined that cephalosporins with lower electron density on the nitrogen atom of the N-O bond, higher number of hydrogen bond-accepting groups, and higher principal moment of inertia should express high adsorption on the mercury electrode.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Voltammetric and theoretical studies of the electrochemical behavior of cephalosporins at a mercury electrode
VL  - 80
IS  - 8
SP  - 1035
EP  - 1049
DO  - 10.2298/JSC150129019N
ER  - 

@article{
author = "Nikolić, Katarina and Aleksić, Mara and Kapetanović, Vera and Agbaba, Danica",
year = "2015",
abstract = "The adsorption and electroreduction behavior of cefpodoxime proxetil, cefotaxime, desacetylcefotaxime, cefetamet, ceftriaxone, ceftazidime, and cefuroxime axetil at a mercury electrode surface were studied using cyclic (CV), differential pulse (DPV) and adsorptive stripping differential pulse (AdSDPV) voltammetry. The quantitative structure property relationship (QSPR) study of the seven cephalosporins adsorption at the mercury electrode was based on density functional theory DFT-B3LYP/6-31G(d,p) calculations of molecular orbitals, partial charges and electron densities of the analytes. The DFT-parameters and QSPR model explain well the process of adsorption of the examined cephalosporins. The QSPR study defined that cephalosporins with lower electron density on the nitrogen atom of the N-O bond, higher number of hydrogen bond-accepting groups, and higher principal moment of inertia should express high adsorption on the mercury electrode.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Voltammetric and theoretical studies of the electrochemical behavior of cephalosporins at a mercury electrode",
volume = "80",
number = "8",
pages = "1035-1049",
doi = "10.2298/JSC150129019N"
}

Nikolić, K., Aleksić, M., Kapetanović, V.,& Agbaba, D.. (2015). Voltammetric and theoretical studies of the electrochemical behavior of cephalosporins at a mercury electrode. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 80(8), 1035-1049.
https://doi.org/10.2298/JSC150129019N

Nikolić K, Aleksić M, Kapetanović V, Agbaba D. Voltammetric and theoretical studies of the electrochemical behavior of cephalosporins at a mercury electrode. in Journal of the Serbian Chemical Society. 2015;80(8):1035-1049.
doi:10.2298/JSC150129019N .

Nikolić, Katarina, Aleksić, Mara, Kapetanović, Vera, Agbaba, Danica, "Voltammetric and theoretical studies of the electrochemical behavior of cephalosporins at a mercury electrode" in Journal of the Serbian Chemical Society, 80, no. 8 (2015):1035-1049,
https://doi.org/10.2298/JSC150129019N . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Pantić, Jelena
AU  - Kapetanović, Vera
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2295
AB  - The electrochemical behavior of a biologically important heterocyclic compound quinoxaline (QUI) was investigated by cyclic voltammetry (CV) in solutions of differing pH, using a glassy carbon electrode (GCE). The reduction of QUI occurs as a quasi-reversible reaction in acid medium, reaching reversibility in alkaline solutions. The kinetic parameters of the electrode process such as αnα, diffusion coefficient (D) and heterogeneous rate constant (ks), were evaluated and discussed. Redox mechanism of QUI was proposed on the basis of experimental results. Reduction process involves a transfer of two electrons and two protons at the pyrazine ring of QUI forming a dihydro-derivative. In acid solutions, the product of QUI reduction undergoes irreversible oxidation in a one-electron process. The electrode processes was found to be diffusion controlled.
AB  - Elektrohemijsko ponašanje biološki značajnog heterocikličnog jedinjenja hinoksalina (QUI) ispitivano je cikličnom voltametrijom (CV) u rastvorima različitih pH vrednosti, korišćenjem electrode odstaklastog ugljenika. Redukcija QUI u kiseloj sredini se odigrava kao kvazi-reverzibilna reakcija, koja u baznoj sredini postaje reverzibilna. Određeni su i razmatrani kinetički parametric elektrodnog procesa kao što su koeficijent αnα, difuzioni koeficijent (D) i konstanta brzine (ks). Na osnovu eksperimentalnih rezultata predložen je mehanizam elektrodne reakcije. U procesu redukcije adiraju se dva elektrona i dva protona na pirazinski prsten hinoksalina i nastaje njegov dihidro derivat. Proizvod redukcije hinoksalina u kiseloj sredini se dalje oksiduje. Ovaj process oksidacije je difuziono kontrolisan, ireverzibilan i odigrava se uz učešće jednog elektrona i jednog protona.
PB  - Univerzitet u Nišu, Niš
T2  - Facta universitatis - series: Physics, Chemistry and Technology
T1  - Evaluation of kinetic parameters and redox mechanism of quinoxaline at glassy carbon electrode
T1  - Određivanje kinetičkih parametara i ispitivanje redoks mehanizma hinoksalina na elektrodi od staklastog ugljenika
VL  - 12
IS  - 1
SP  - 55
EP  - 63
DO  - 10.2298/FUPCT1401055A
ER  - 

@article{
author = "Aleksić, Mara and Pantić, Jelena and Kapetanović, Vera",
year = "2014",
abstract = "The electrochemical behavior of a biologically important heterocyclic compound quinoxaline (QUI) was investigated by cyclic voltammetry (CV) in solutions of differing pH, using a glassy carbon electrode (GCE). The reduction of QUI occurs as a quasi-reversible reaction in acid medium, reaching reversibility in alkaline solutions. The kinetic parameters of the electrode process such as αnα, diffusion coefficient (D) and heterogeneous rate constant (ks), were evaluated and discussed. Redox mechanism of QUI was proposed on the basis of experimental results. Reduction process involves a transfer of two electrons and two protons at the pyrazine ring of QUI forming a dihydro-derivative. In acid solutions, the product of QUI reduction undergoes irreversible oxidation in a one-electron process. The electrode processes was found to be diffusion controlled., Elektrohemijsko ponašanje biološki značajnog heterocikličnog jedinjenja hinoksalina (QUI) ispitivano je cikličnom voltametrijom (CV) u rastvorima različitih pH vrednosti, korišćenjem electrode odstaklastog ugljenika. Redukcija QUI u kiseloj sredini se odigrava kao kvazi-reverzibilna reakcija, koja u baznoj sredini postaje reverzibilna. Određeni su i razmatrani kinetički parametric elektrodnog procesa kao što su koeficijent αnα, difuzioni koeficijent (D) i konstanta brzine (ks). Na osnovu eksperimentalnih rezultata predložen je mehanizam elektrodne reakcije. U procesu redukcije adiraju se dva elektrona i dva protona na pirazinski prsten hinoksalina i nastaje njegov dihidro derivat. Proizvod redukcije hinoksalina u kiseloj sredini se dalje oksiduje. Ovaj process oksidacije je difuziono kontrolisan, ireverzibilan i odigrava se uz učešće jednog elektrona i jednog protona.",
publisher = "Univerzitet u Nišu, Niš",
journal = "Facta universitatis - series: Physics, Chemistry and Technology",
title = "Evaluation of kinetic parameters and redox mechanism of quinoxaline at glassy carbon electrode, Određivanje kinetičkih parametara i ispitivanje redoks mehanizma hinoksalina na elektrodi od staklastog ugljenika",
volume = "12",
number = "1",
pages = "55-63",
doi = "10.2298/FUPCT1401055A"
}

Aleksić, M., Pantić, J.,& Kapetanović, V.. (2014). Evaluation of kinetic parameters and redox mechanism of quinoxaline at glassy carbon electrode. in Facta universitatis - series: Physics, Chemistry and Technology
Univerzitet u Nišu, Niš., 12(1), 55-63.
https://doi.org/10.2298/FUPCT1401055A

Aleksić M, Pantić J, Kapetanović V. Evaluation of kinetic parameters and redox mechanism of quinoxaline at glassy carbon electrode. in Facta universitatis - series: Physics, Chemistry and Technology. 2014;12(1):55-63.
doi:10.2298/FUPCT1401055A .

Aleksić, Mara, Pantić, Jelena, Kapetanović, Vera, "Evaluation of kinetic parameters and redox mechanism of quinoxaline at glassy carbon electrode" in Facta universitatis - series: Physics, Chemistry and Technology, 12, no. 1 (2014):55-63,
https://doi.org/10.2298/FUPCT1401055A . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2138
AB  - A large number of inorganic and organic compounds is able to bind to DNA and form complexes. Among them, drugs are very important, especially chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and non-covalent) between DNA molecule and drugs. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, non-covalent binding is reversible and based on the principle of molecular recognition. Special attention is given to elucidation of the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react non-covalently with DNA are mainly intercalators, but also minor and major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the study of the interactions between DNA and drugs with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, fluorescence, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, and fluorescence anisotropy or resonance is used. The development of the electrochemical DNA biosensors has opened a wide perspective using particularly sensitive and selective electrochemical methods for the detection of specific DNA interactions. The presented results summarize literature data obtained by the mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions
VL  - 61
IS  - 3
SP  - 555
EP  - 573
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2138
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2014",
abstract = "A large number of inorganic and organic compounds is able to bind to DNA and form complexes. Among them, drugs are very important, especially chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and non-covalent) between DNA molecule and drugs. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, non-covalent binding is reversible and based on the principle of molecular recognition. Special attention is given to elucidation of the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react non-covalently with DNA are mainly intercalators, but also minor and major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the study of the interactions between DNA and drugs with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, fluorescence, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, and fluorescence anisotropy or resonance is used. The development of the electrochemical DNA biosensors has opened a wide perspective using particularly sensitive and selective electrochemical methods for the detection of specific DNA interactions. The presented results summarize literature data obtained by the mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions",
volume = "61",
number = "3",
pages = "555-573",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2138"
}

Aleksić, M.,& Kapetanović, V.. (2014). An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 61(3), 555-573.
https://hdl.handle.net/21.15107/rcub_farfar_2138

Aleksić M, Kapetanović V. An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions. in Acta Chimica Slovenica. 2014;61(3):555-573.
https://hdl.handle.net/21.15107/rcub_farfar_2138 .

Aleksić, Mara, Kapetanović, Vera, "An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions" in Acta Chimica Slovenica, 61, no. 3 (2014):555-573,
https://hdl.handle.net/21.15107/rcub_farfar_2138 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2017
AB  - Large number of inorganic and organic compounds is able to bind to DNA and form the complex. Among them, very important drugs are chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and noncovalent) between drugs and DNA molecule. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, noncovalent binding is reversible and based on the principle of molecular recognition. Special attention is paid to explain the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react noncovalently with DNA are intercalators, minor or major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the investigation of the interactions between drug and DNA with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, fluorescence anisotropy or resonance, or very sensitive DNA-biosensors are used. The presented results summarize literature data obtained by the use of mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.
AB  - Komplekse sa dezoksiribonukleinskom kiselinom (DNK) gradi veliki broj neorganskih i organskih jedinjenja, među kojima su od posebnog značaja lekovi iz grupe hemioterapeutika. U radu je dat pregled strukturnih karakteristika DNK molekula i tipova interakcije (kovalentne i nekovalentne) koje se javljaju između molekula leka i DNK. Kovalentno vezivanje leka za DNK je ireverzibilno i vodi ka kompletnoj inhibiciji funkcija DNK što dovodi do smrti ćelije, dok je nekovalentno vezivanje reverzibilno i zasniva se na principu molekularnog prepoznavanja. Posebna pažnja je posvećena objašnjenju specifičnih mesta u molekulu DNK Na kojima dolazi do vezivanja leka, u zavisnosti od strukturnih karakteristika molekula leka. Najveći broj lekova koji reaguju nekovalentno su interkalatni agensi, a pored njih postoje i lekovi koji se vezuju za mali ili veliki žljeb molekula DNK. Prilikom građenja ovih kompleksa nastaju promene kako na molekulu DNK tako i na molekulu leka. U radu je dat pregled metoda koje se koriste u ispitivanju interakcija između leka i DNK sa ciljem detekcije i objašnjenja nastalih promena. U ovu svrhu koriste se spektroskopske metode, kao što su UV/VIS, infracrvena, ramanska i NMR spektroskopija, zatim spektroskopije polarizovane svetlosti: metode linearnog i cirkularnog dihroizma, fluorescentne anizotropije ili rezonancije, a u novije vreme i osetljivi DNK-biosenzori. Predstavljeni su literaturni rezultati dobijeni primenom navedenih metoda koji se koriste za utvrđivanje oštećenja na DNK molekulu, određivanje mesta specifičnog vezivanja leka, redosleda vezivanja, kao i za detekciju konformacionih promena nastalih usled lek-DNK interakcije.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Drug - DNA interactions: Properties and detection
T1  - Interakcije lekova i DNK- osobine i detekcija
VL  - 63
IS  - 3
SP  - 279
EP  - 292
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2017
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2013",
abstract = "Large number of inorganic and organic compounds is able to bind to DNA and form the complex. Among them, very important drugs are chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and noncovalent) between drugs and DNA molecule. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, noncovalent binding is reversible and based on the principle of molecular recognition. Special attention is paid to explain the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react noncovalently with DNA are intercalators, minor or major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the investigation of the interactions between drug and DNA with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, fluorescence anisotropy or resonance, or very sensitive DNA-biosensors are used. The presented results summarize literature data obtained by the use of mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction., Komplekse sa dezoksiribonukleinskom kiselinom (DNK) gradi veliki broj neorganskih i organskih jedinjenja, među kojima su od posebnog značaja lekovi iz grupe hemioterapeutika. U radu je dat pregled strukturnih karakteristika DNK molekula i tipova interakcije (kovalentne i nekovalentne) koje se javljaju između molekula leka i DNK. Kovalentno vezivanje leka za DNK je ireverzibilno i vodi ka kompletnoj inhibiciji funkcija DNK što dovodi do smrti ćelije, dok je nekovalentno vezivanje reverzibilno i zasniva se na principu molekularnog prepoznavanja. Posebna pažnja je posvećena objašnjenju specifičnih mesta u molekulu DNK Na kojima dolazi do vezivanja leka, u zavisnosti od strukturnih karakteristika molekula leka. Najveći broj lekova koji reaguju nekovalentno su interkalatni agensi, a pored njih postoje i lekovi koji se vezuju za mali ili veliki žljeb molekula DNK. Prilikom građenja ovih kompleksa nastaju promene kako na molekulu DNK tako i na molekulu leka. U radu je dat pregled metoda koje se koriste u ispitivanju interakcija između leka i DNK sa ciljem detekcije i objašnjenja nastalih promena. U ovu svrhu koriste se spektroskopske metode, kao što su UV/VIS, infracrvena, ramanska i NMR spektroskopija, zatim spektroskopije polarizovane svetlosti: metode linearnog i cirkularnog dihroizma, fluorescentne anizotropije ili rezonancije, a u novije vreme i osetljivi DNK-biosenzori. Predstavljeni su literaturni rezultati dobijeni primenom navedenih metoda koji se koriste za utvrđivanje oštećenja na DNK molekulu, određivanje mesta specifičnog vezivanja leka, redosleda vezivanja, kao i za detekciju konformacionih promena nastalih usled lek-DNK interakcije.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Drug - DNA interactions: Properties and detection, Interakcije lekova i DNK- osobine i detekcija",
volume = "63",
number = "3",
pages = "279-292",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2017"
}

Aleksić, M.,& Kapetanović, V.. (2013). Drug - DNA interactions: Properties and detection. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 63(3), 279-292.
https://hdl.handle.net/21.15107/rcub_farfar_2017

Aleksić M, Kapetanović V. Drug - DNA interactions: Properties and detection. in Arhiv za farmaciju. 2013;63(3):279-292.
https://hdl.handle.net/21.15107/rcub_farfar_2017 .

Aleksić, Mara, Kapetanović, Vera, "Drug - DNA interactions: Properties and detection" in Arhiv za farmaciju, 63, no. 3 (2013):279-292,
https://hdl.handle.net/21.15107/rcub_farfar_2017 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Lijeskić, Nikola
AU  - Pantić, Jelena
AU  - Kapetanović, Vera
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2009
AB  - The voltammetric behavior of three cephalosporins: ceftazidime, cefuroxime-axetil and ceftriaxone has been examined in pH range 2.0-8.0 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV), using a hanging mercury drop electrode (HMDE). The effect of pH of the electrolyte solution and scan rate on the peak currents and peak potentials was examined. The nature of the electrode reduction process in acid solution was found to be diffusion controlled for ceftazidime and cefuroxime-axetil, but strongly influenced by adsorption in the case of ceftriaxone reduction. The adsorption and reorientation of the ceftriaxone molecule at the electrode surface caused instability of the voltammetric signal and disabled its determination in the acid medium. Ceftriaxone adsorption decreased with the increase of pH, and at pH>7 the reduction process became diffusion controlled. Based on this study, DPV method was developed, validated and suggested for determination of ceftazidime at pH 2.0, cefuroxime-axetil at pH 3.5 and for ceftriaxone at pH 8.0. Linear concentration ranges, limits of detection (LOD) and quantification (LOQ) were determined. The method was applied for determination of cephalosporins in pharmaceutical dosage forms: Ceftazidime powder, Ceroxim tablets and Longaceph powder for injection solution.
AB  - Voltametrijsko ponašanje tri cephalosporina: ceftazidima, cefuroksim-aksetila i ceftriaksona ispitivano je cikličnom (CV) i diferencijalno pulsnom (DPV) voltametrijom u pH oblasti od 2,0 do 8,0 na visećoj živnoj kapi. Razmatrani su uticaji pH osnovnog elektrolita i brzine promene potencijala na vrednost stuje i potencijala voltametrijskih pikova. Rezultati dobijeni u kiseloj sredini su pokazali da je redukcija ceftazidima i cefuroksim-aksetila difuziono kontrolisan proces, a da je u slučaju ceftriaksona priroda redukcije na elektrodi jako zavisna od adsorpcije. Posledica adsorpcije i reorijentacije molekula ceftriaksona na površini elektrode je nestabilnost i nereproduktivnost voltametrijskog signala što onemogućava određivanje cefriaksona u kiseloj sredini. Intenzitet adsorpcije ceftriaksona opada sa porastom pH i pri pH>7 njegova redukcija postaje difuzijom kontrolisan proces. Na osnovu ovih rezultata predložena je i validirana DPV metoda za određvanje ceftazidima na pH 2,0, cefuroksim-aksetila na pH 3,5 i ceftriaksona na pH 8,0. Određene su vrednosti opsega linearnosti, granice detekcije i određivanja. Metoda je uspešno primenjena za određivanje ovih cefalosporina u farmaceutskim doziranim oblicima i to u Ceroxim tabletama i Ceftazidim i Longaceph prašku za injekcione rastvore.
PB  - Univerzitet u Nišu, Niš
T2  - Facta universitatis - series: Physics, Chemistry and Technology
T1  - Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone
T1  - Elektrohemijsko ponašanje i primena diferencijalno pulsne voltametrije za određivanje ceftazidima, cefuroksim-aksetila i ceftriaksona
VL  - 11
IS  - 1
SP  - 55
EP  - 66
DO  - 10.2298/FUPCT1301055A
ER  - 

@article{
author = "Aleksić, Mara and Lijeskić, Nikola and Pantić, Jelena and Kapetanović, Vera",
year = "2013",
abstract = "The voltammetric behavior of three cephalosporins: ceftazidime, cefuroxime-axetil and ceftriaxone has been examined in pH range 2.0-8.0 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV), using a hanging mercury drop electrode (HMDE). The effect of pH of the electrolyte solution and scan rate on the peak currents and peak potentials was examined. The nature of the electrode reduction process in acid solution was found to be diffusion controlled for ceftazidime and cefuroxime-axetil, but strongly influenced by adsorption in the case of ceftriaxone reduction. The adsorption and reorientation of the ceftriaxone molecule at the electrode surface caused instability of the voltammetric signal and disabled its determination in the acid medium. Ceftriaxone adsorption decreased with the increase of pH, and at pH>7 the reduction process became diffusion controlled. Based on this study, DPV method was developed, validated and suggested for determination of ceftazidime at pH 2.0, cefuroxime-axetil at pH 3.5 and for ceftriaxone at pH 8.0. Linear concentration ranges, limits of detection (LOD) and quantification (LOQ) were determined. The method was applied for determination of cephalosporins in pharmaceutical dosage forms: Ceftazidime powder, Ceroxim tablets and Longaceph powder for injection solution., Voltametrijsko ponašanje tri cephalosporina: ceftazidima, cefuroksim-aksetila i ceftriaksona ispitivano je cikličnom (CV) i diferencijalno pulsnom (DPV) voltametrijom u pH oblasti od 2,0 do 8,0 na visećoj živnoj kapi. Razmatrani su uticaji pH osnovnog elektrolita i brzine promene potencijala na vrednost stuje i potencijala voltametrijskih pikova. Rezultati dobijeni u kiseloj sredini su pokazali da je redukcija ceftazidima i cefuroksim-aksetila difuziono kontrolisan proces, a da je u slučaju ceftriaksona priroda redukcije na elektrodi jako zavisna od adsorpcije. Posledica adsorpcije i reorijentacije molekula ceftriaksona na površini elektrode je nestabilnost i nereproduktivnost voltametrijskog signala što onemogućava određivanje cefriaksona u kiseloj sredini. Intenzitet adsorpcije ceftriaksona opada sa porastom pH i pri pH>7 njegova redukcija postaje difuzijom kontrolisan proces. Na osnovu ovih rezultata predložena je i validirana DPV metoda za određvanje ceftazidima na pH 2,0, cefuroksim-aksetila na pH 3,5 i ceftriaksona na pH 8,0. Određene su vrednosti opsega linearnosti, granice detekcije i određivanja. Metoda je uspešno primenjena za određivanje ovih cefalosporina u farmaceutskim doziranim oblicima i to u Ceroxim tabletama i Ceftazidim i Longaceph prašku za injekcione rastvore.",
publisher = "Univerzitet u Nišu, Niš",
journal = "Facta universitatis - series: Physics, Chemistry and Technology",
title = "Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone, Elektrohemijsko ponašanje i primena diferencijalno pulsne voltametrije za određivanje ceftazidima, cefuroksim-aksetila i ceftriaksona",
volume = "11",
number = "1",
pages = "55-66",
doi = "10.2298/FUPCT1301055A"
}

Aleksić, M., Lijeskić, N., Pantić, J.,& Kapetanović, V.. (2013). Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone. in Facta universitatis - series: Physics, Chemistry and Technology
Univerzitet u Nišu, Niš., 11(1), 55-66.
https://doi.org/10.2298/FUPCT1301055A

Aleksić M, Lijeskić N, Pantić J, Kapetanović V. Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone. in Facta universitatis - series: Physics, Chemistry and Technology. 2013;11(1):55-66.
doi:10.2298/FUPCT1301055A .

Aleksić, Mara, Lijeskić, Nikola, Pantić, Jelena, Kapetanović, Vera, "Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone" in Facta universitatis - series: Physics, Chemistry and Technology, 11, no. 1 (2013):55-66,
https://doi.org/10.2298/FUPCT1301055A . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2007
AB  - In recent years, a great progress was achieved in the development of electrochemical sensors for DNA sequences, hybridization and damage. Nowadays, electrochemical methods are able to detect DNA at nanomolar concentration. In addition, these methods are suitable for studding both covalent and non-covalent binding interactions between DNA and different small molecules, e.g. drugs or potentially mutagenic agents. This suggests that electrochemical biosensors might become important tools in medical research. The aim of this review is to draw attention to the applicability of different electrochemical techniques for studying interactions between DNA with other molecules, and in the design of new sensitive and selective biosensors.
AB  - Poslednjih godina je postignut veliki napredak u razvoju elektrohemijskih biosenzora za ispitivanja sekvenci, hibridizacije i oštećenja na molekulu DNK. Primenom elektrohemijskih metoda se u današnje vreme mogu detektovati i određivati nanomolarne koncentracije DNK. Pored toga, ove metode su pogodne za ispitivanje kako kovalentnih, tako i nekovalentnih interakcija između DNK i različitih malih molekula, kakvi su lekovi i drugi potencijalno mutageni agensi. Ovo sugeriše da bi elektrohemijski biosenzori mogli biti od značaja i u oblast medicinskih istraživanja. Cilj ovoga preglednog rada je da skrene pažnju na primenljivost različitih elektrohemijskih metoda za proučavanje interakcija između DNK i drugih molekula, kao i u izradu novih osetljivih i selektivnih biosenzora.
PB  - Univerzitet u Nišu, Niš
T2  - Facta universitatis - series: Physics, Chemistry and Technology
T1  - Electrochemical biosensors as a tool for the investigation of DNA structure, damage and interaction with other molecules
T1  - Primena elektrohemijskih biosenzora za ispitivanje strukture, oštećenja i interakcija DNK sa drugim molekulima
VL  - 11
IS  - 1
SP  - 27
EP  - 43
DO  - 10.2298/FUPCT1301027A
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2013",
abstract = "In recent years, a great progress was achieved in the development of electrochemical sensors for DNA sequences, hybridization and damage. Nowadays, electrochemical methods are able to detect DNA at nanomolar concentration. In addition, these methods are suitable for studding both covalent and non-covalent binding interactions between DNA and different small molecules, e.g. drugs or potentially mutagenic agents. This suggests that electrochemical biosensors might become important tools in medical research. The aim of this review is to draw attention to the applicability of different electrochemical techniques for studying interactions between DNA with other molecules, and in the design of new sensitive and selective biosensors., Poslednjih godina je postignut veliki napredak u razvoju elektrohemijskih biosenzora za ispitivanja sekvenci, hibridizacije i oštećenja na molekulu DNK. Primenom elektrohemijskih metoda se u današnje vreme mogu detektovati i određivati nanomolarne koncentracije DNK. Pored toga, ove metode su pogodne za ispitivanje kako kovalentnih, tako i nekovalentnih interakcija između DNK i različitih malih molekula, kakvi su lekovi i drugi potencijalno mutageni agensi. Ovo sugeriše da bi elektrohemijski biosenzori mogli biti od značaja i u oblast medicinskih istraživanja. Cilj ovoga preglednog rada je da skrene pažnju na primenljivost različitih elektrohemijskih metoda za proučavanje interakcija između DNK i drugih molekula, kao i u izradu novih osetljivih i selektivnih biosenzora.",
publisher = "Univerzitet u Nišu, Niš",
journal = "Facta universitatis - series: Physics, Chemistry and Technology",
title = "Electrochemical biosensors as a tool for the investigation of DNA structure, damage and interaction with other molecules, Primena elektrohemijskih biosenzora za ispitivanje strukture, oštećenja i interakcija DNK sa drugim molekulima",
volume = "11",
number = "1",
pages = "27-43",
doi = "10.2298/FUPCT1301027A"
}

Aleksić, M.,& Kapetanović, V.. (2013). Electrochemical biosensors as a tool for the investigation of DNA structure, damage and interaction with other molecules. in Facta universitatis - series: Physics, Chemistry and Technology
Univerzitet u Nišu, Niš., 11(1), 27-43.
https://doi.org/10.2298/FUPCT1301027A

Aleksić M, Kapetanović V. Electrochemical biosensors as a tool for the investigation of DNA structure, damage and interaction with other molecules. in Facta universitatis - series: Physics, Chemistry and Technology. 2013;11(1):27-43.
doi:10.2298/FUPCT1301027A .

Aleksić, Mara, Kapetanović, Vera, "Electrochemical biosensors as a tool for the investigation of DNA structure, damage and interaction with other molecules" in Facta universitatis - series: Physics, Chemistry and Technology, 11, no. 1 (2013):27-43,
https://doi.org/10.2298/FUPCT1301027A . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Radulović, Valentina
AU  - Agbaba, Danica
AU  - Kapetanović, Vera
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1979
AB  - The electrochemical behavior of brimonidine (BRIM), an antiglaucoma agent applied in therapy for lowering high intraocular pressure, was investigated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry using a glassy carbon electrode (GCE). The reduction of BRIM occurs as one-step quasi-reversible reaction in acid and neutral medium, reaching the full reversibility in alkaline solutions. Reduction process involves the transfer of two electrons and two protons at the pyrazine ring of quinoxaline moiety, forming a dihydro-derivative. In acid and neutral solutions, brimonidine reduction product is partly oxidized to its hydroxy-derivative. BRIM is also oxidized irreversibly with the transfer of one electron and one proton at secondary amine moiety. The effects of pH of the electrolyte solution, scan rate and BRIM concentration were monitored. The nature of the electrode process was found to be controlled by the adsorption at pH > 6 and the total surface concentration of brimonidine adsorbed onto the GCE surface at pH 7, Gamma(BRIM) = 1.35 x 10(-10) mol cm(-2) was obtained. Based on this study, differential pulse voltammetric method was developed, validated and suggested for rapid electroanalytical determination of the low concentration of brimonidine. The linearity was achieved within the concentration range from 5 x 10(-7) to 5 x 10(-6) M with LOD = 1.6 x 10(-7) M and LOQ = 5.3 x 10(-7) M. The method was applied for brimonidine determination in pharmaceutical dosage form, eye drops.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode
VL  - 106
SP  - 75
EP  - 81
DO  - 10.1016/j.electacta.2013.05.053
ER  - 

@article{
author = "Aleksić, Mara and Radulović, Valentina and Agbaba, Danica and Kapetanović, Vera",
year = "2013",
abstract = "The electrochemical behavior of brimonidine (BRIM), an antiglaucoma agent applied in therapy for lowering high intraocular pressure, was investigated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry using a glassy carbon electrode (GCE). The reduction of BRIM occurs as one-step quasi-reversible reaction in acid and neutral medium, reaching the full reversibility in alkaline solutions. Reduction process involves the transfer of two electrons and two protons at the pyrazine ring of quinoxaline moiety, forming a dihydro-derivative. In acid and neutral solutions, brimonidine reduction product is partly oxidized to its hydroxy-derivative. BRIM is also oxidized irreversibly with the transfer of one electron and one proton at secondary amine moiety. The effects of pH of the electrolyte solution, scan rate and BRIM concentration were monitored. The nature of the electrode process was found to be controlled by the adsorption at pH > 6 and the total surface concentration of brimonidine adsorbed onto the GCE surface at pH 7, Gamma(BRIM) = 1.35 x 10(-10) mol cm(-2) was obtained. Based on this study, differential pulse voltammetric method was developed, validated and suggested for rapid electroanalytical determination of the low concentration of brimonidine. The linearity was achieved within the concentration range from 5 x 10(-7) to 5 x 10(-6) M with LOD = 1.6 x 10(-7) M and LOQ = 5.3 x 10(-7) M. The method was applied for brimonidine determination in pharmaceutical dosage form, eye drops.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode",
volume = "106",
pages = "75-81",
doi = "10.1016/j.electacta.2013.05.053"
}

Aleksić, M., Radulović, V., Agbaba, D.,& Kapetanović, V.. (2013). An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford., 106, 75-81.
https://doi.org/10.1016/j.electacta.2013.05.053

Aleksić M, Radulović V, Agbaba D, Kapetanović V. An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode. in Electrochimica Acta. 2013;106:75-81.
doi:10.1016/j.electacta.2013.05.053 .

Aleksić, Mara, Radulović, Valentina, Agbaba, Danica, Kapetanović, Vera, "An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode" in Electrochimica Acta, 106 (2013):75-81,
https://doi.org/10.1016/j.electacta.2013.05.053 . .

TY  - JOUR
AU  - Radulović, Valentina
AU  - Aleksić, Mara
AU  - Agbaba, Danica
AU  - Kapetanović, Vera
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1951
AB  - An extensive electrochemical study of brimonidine at boron doped diamond electrode (BDDE) was done by applying cyclic voltammetry (CV) and square-wave voltammetry (SWV) in sulfuric acid of different concentrations (pH ranged from 0.6 to 1.6), and in BR buffer (pH ranged from 2.0 to 9.0). It was found that the reduction of brimonidine occurred in a one-step quasi-reversible mechanism, involving the transfer of two electrons and two protons. The reduction process took place at the quinoxaline ring and the corresponding mechanism of reduction was confirmed to be the same as for the other quinoxaline derivatives. The nature of the electrode process was found to be diffusion controlled in acid medium, while in a more alkaline medium a certain degree of adsorption was noticed. Based on this study, two sensitive voltammetric methods, differential pulse (DPV) and square wave (SWV) were developed, fully validated and suggested for rapid electroanalytical determination of low concentrations of brimonidine. The linearity was achieved within the concentration range from 2x10-6 M to 3x10-5 M for DPV (LOD=6.31x10-7 M, LOQ=2.1x10-6 M) and from 5x10-7 M to 1.5x10-5 M for SWV (LOD=1.28x10-7 M, LOQ=4.28x10-7 M). The methods were applied for brimonidine determination in pharmaceutical dosage form, eye drops. The obtained good recoveries suggested these simple and accurate methods for quality control of brimonidine in dosage form. Due to its high sensitivity, the SWV method could be a good alternative for determination of low concentrations of brimonidine, even in biological samples.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Electroanalysis
T1  - An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study
VL  - 25
IS  - 1
SP  - 230
EP  - 236
DO  - 10.1002/elan.201200400
ER  - 

@article{
author = "Radulović, Valentina and Aleksić, Mara and Agbaba, Danica and Kapetanović, Vera",
year = "2013",
abstract = "An extensive electrochemical study of brimonidine at boron doped diamond electrode (BDDE) was done by applying cyclic voltammetry (CV) and square-wave voltammetry (SWV) in sulfuric acid of different concentrations (pH ranged from 0.6 to 1.6), and in BR buffer (pH ranged from 2.0 to 9.0). It was found that the reduction of brimonidine occurred in a one-step quasi-reversible mechanism, involving the transfer of two electrons and two protons. The reduction process took place at the quinoxaline ring and the corresponding mechanism of reduction was confirmed to be the same as for the other quinoxaline derivatives. The nature of the electrode process was found to be diffusion controlled in acid medium, while in a more alkaline medium a certain degree of adsorption was noticed. Based on this study, two sensitive voltammetric methods, differential pulse (DPV) and square wave (SWV) were developed, fully validated and suggested for rapid electroanalytical determination of low concentrations of brimonidine. The linearity was achieved within the concentration range from 2x10-6 M to 3x10-5 M for DPV (LOD=6.31x10-7 M, LOQ=2.1x10-6 M) and from 5x10-7 M to 1.5x10-5 M for SWV (LOD=1.28x10-7 M, LOQ=4.28x10-7 M). The methods were applied for brimonidine determination in pharmaceutical dosage form, eye drops. The obtained good recoveries suggested these simple and accurate methods for quality control of brimonidine in dosage form. Due to its high sensitivity, the SWV method could be a good alternative for determination of low concentrations of brimonidine, even in biological samples.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Electroanalysis",
title = "An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study",
volume = "25",
number = "1",
pages = "230-236",
doi = "10.1002/elan.201200400"
}

Radulović, V., Aleksić, M., Agbaba, D.,& Kapetanović, V.. (2013). An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study. in Electroanalysis
Wiley-VCH Verlag GMBH, Weinheim., 25(1), 230-236.
https://doi.org/10.1002/elan.201200400

Radulović V, Aleksić M, Agbaba D, Kapetanović V. An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study. in Electroanalysis. 2013;25(1):230-236.
doi:10.1002/elan.201200400 .

Radulović, Valentina, Aleksić, Mara, Agbaba, Danica, Kapetanović, Vera, "An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study" in Electroanalysis, 25, no. 1 (2013):230-236,
https://doi.org/10.1002/elan.201200400 . .

TY  - JOUR
AU  - Radulović, Valentina
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1817
AB  - The electrochemical behaviour of a novel nicotinic α4β2 subtype receptor partial agonist varenicline (VAR), which is used for smoking cessation, was investigated in Britton-Robinson buffers (pH 2.0-12.0) by cyclic, differential pulse and square wave voltammetry at a hanging mercury drop electrode (HMDE). The influence of pH, scan rate, concentration, accumulation potential and time on the peak current and potential suggested that the redox process was adsorption controlled in alkaline media. In addition, the experimental value of the surface coverage, G = 1.03×10-10 mol cm-2, was used to determine the conditions when VAR was fully adsorbed at the electrode surface. Bearing in mind the potential high toxicity of VAR due to the presence of a quinoxaline structure, its interaction with double stranded-DNA (ds-DNA) was postulated and studied when both compounds were in the adsorbed state at a modified HMDE. Using the adsorptive transfer technique, changes in potential and decreases in the normalized peak currents were observed. The estimated value of the ratio of surface-binding constants indicated that the reduced form of VAR interacted with ds-DNA more strongly than the oxidized form. Subtle DNA damage under conditions of direct DNA-VAR interaction at room temperature was observed. The proposed type of interaction was intercalation. This study employed a simple electroanalytical methodology and showed the potential of a DNA/ /HMDE biosensor for investigation of genotoxic effects.
AB  - Elektrohemijsko ponašanje vareniklina, novog parcijalnog agoniste α4β2 nikotinskog receptora, koji se koristi za odvikavanje od pušenja, ispitano je cikličnom, diferencijalno pulsnom i voltametrijom pravougaonih talasa u Briton-Robinsonovom puferu (pH 2,0-12,0). Na osnovu uticaja pH, brzine promene potencijala, koncentracije, potencijala i vremena akumulacije na visinu i položaj pika, zaključeno je da je proces redukcije u alkalnoj sredini kontrolisan adsorpcijom vareniklina. Korišćenjem eksperimentalno dobijene vrednosti površinske zaposednutosti, G = 1,03×10-10 mol cm-2, određeni su uslovi pod kojima je vareniklin potpuno adsorbovan na površini elektrode. Imajući u vidu moguću visoku toksičnost vareniklina, s obzirom na prisustvo hinoksalinskog prstena u strukturi, pretpostavljeno je da vareniklin interaguje sa DNK kada su oba molekula adsorbovana na modifikovanoj živinoj elektrodi. Ova interakcija je ispitana korišćenjem 'adsorptivne transfer tehnike' i primećene su promene potencijala i smanjenje normalizovanih struja voltametrijskih pikova. Na osnovu izračunate vrednosti odnosa konstanti vezivanja zaključeno je da se redukovani oblik vareniklina jače vezuje za DNK od oksidovanog. Pretpostavljeno je da direktna vareniklin-DNK interakcija na sobnoj temperaturi dovodi do izvesnog oštećenja DNK i da je tip interakcije - interkalacija. Ova jednostavna elektroanalitička metodologija mogla bi naći primenu u vidu potencijalnog biosenzora za ispitivanje genotoksičnih efekata.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - An electrochemical study of the adsorptive behaviour of varenicline and its interaction with DNA
T1  - Elektrohemijsko ispitivanje adsorpcije i interakcije vareniklina i DNK
VL  - 77
IS  - 10
SP  - 1409
EP  - 1422
DO  - 10.2298/JSC120420073R
ER  - 

@article{
author = "Radulović, Valentina and Aleksić, Mara and Kapetanović, Vera",
year = "2012",
abstract = "The electrochemical behaviour of a novel nicotinic α4β2 subtype receptor partial agonist varenicline (VAR), which is used for smoking cessation, was investigated in Britton-Robinson buffers (pH 2.0-12.0) by cyclic, differential pulse and square wave voltammetry at a hanging mercury drop electrode (HMDE). The influence of pH, scan rate, concentration, accumulation potential and time on the peak current and potential suggested that the redox process was adsorption controlled in alkaline media. In addition, the experimental value of the surface coverage, G = 1.03×10-10 mol cm-2, was used to determine the conditions when VAR was fully adsorbed at the electrode surface. Bearing in mind the potential high toxicity of VAR due to the presence of a quinoxaline structure, its interaction with double stranded-DNA (ds-DNA) was postulated and studied when both compounds were in the adsorbed state at a modified HMDE. Using the adsorptive transfer technique, changes in potential and decreases in the normalized peak currents were observed. The estimated value of the ratio of surface-binding constants indicated that the reduced form of VAR interacted with ds-DNA more strongly than the oxidized form. Subtle DNA damage under conditions of direct DNA-VAR interaction at room temperature was observed. The proposed type of interaction was intercalation. This study employed a simple electroanalytical methodology and showed the potential of a DNA/ /HMDE biosensor for investigation of genotoxic effects., Elektrohemijsko ponašanje vareniklina, novog parcijalnog agoniste α4β2 nikotinskog receptora, koji se koristi za odvikavanje od pušenja, ispitano je cikličnom, diferencijalno pulsnom i voltametrijom pravougaonih talasa u Briton-Robinsonovom puferu (pH 2,0-12,0). Na osnovu uticaja pH, brzine promene potencijala, koncentracije, potencijala i vremena akumulacije na visinu i položaj pika, zaključeno je da je proces redukcije u alkalnoj sredini kontrolisan adsorpcijom vareniklina. Korišćenjem eksperimentalno dobijene vrednosti površinske zaposednutosti, G = 1,03×10-10 mol cm-2, određeni su uslovi pod kojima je vareniklin potpuno adsorbovan na površini elektrode. Imajući u vidu moguću visoku toksičnost vareniklina, s obzirom na prisustvo hinoksalinskog prstena u strukturi, pretpostavljeno je da vareniklin interaguje sa DNK kada su oba molekula adsorbovana na modifikovanoj živinoj elektrodi. Ova interakcija je ispitana korišćenjem 'adsorptivne transfer tehnike' i primećene su promene potencijala i smanjenje normalizovanih struja voltametrijskih pikova. Na osnovu izračunate vrednosti odnosa konstanti vezivanja zaključeno je da se redukovani oblik vareniklina jače vezuje za DNK od oksidovanog. Pretpostavljeno je da direktna vareniklin-DNK interakcija na sobnoj temperaturi dovodi do izvesnog oštećenja DNK i da je tip interakcije - interkalacija. Ova jednostavna elektroanalitička metodologija mogla bi naći primenu u vidu potencijalnog biosenzora za ispitivanje genotoksičnih efekata.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "An electrochemical study of the adsorptive behaviour of varenicline and its interaction with DNA, Elektrohemijsko ispitivanje adsorpcije i interakcije vareniklina i DNK",
volume = "77",
number = "10",
pages = "1409-1422",
doi = "10.2298/JSC120420073R"
}

Radulović, V., Aleksić, M.,& Kapetanović, V.. (2012). An electrochemical study of the adsorptive behaviour of varenicline and its interaction with DNA. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(10), 1409-1422.
https://doi.org/10.2298/JSC120420073R

Radulović V, Aleksić M, Kapetanović V. An electrochemical study of the adsorptive behaviour of varenicline and its interaction with DNA. in Journal of the Serbian Chemical Society. 2012;77(10):1409-1422.
doi:10.2298/JSC120420073R .

Radulović, Valentina, Aleksić, Mara, Kapetanović, Vera, "An electrochemical study of the adsorptive behaviour of varenicline and its interaction with DNA" in Journal of the Serbian Chemical Society, 77, no. 10 (2012):1409-1422,
https://doi.org/10.2298/JSC120420073R . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Radulović, Valentina
AU  - Lijeskić, Nikola
AU  - Kapetanović, Vera
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1739
AB  - The electrochemical study of varenicline (VAR) was done in a wide pH range (2-12), at boron doped diamond electrode (BDDE), glassy carbon electrode (GCE) and hanging mercury electrode (HMDE), using cyclic (CV), square wave (SW) and adsorptive stripping square wave (AdSSW) voltammetric techniques. Depending on the pH and the type of the working electrode the characteristic electrochemical behavior of varenicline was established. The mechanism of the reduction process was suggested. Based on the obtained results, the new electroanalytical method was developed for its determination in the buffer solutions and plasma samples. By applying a square wave voltammetry (SWV) on BDDE and GCE, at pH 3.5 and 4.0, the linear dependence in plasma samples was achieved within the concentration range from 2 10(-6) -1 x 10(-5) M and 4 x 10(-6) -1 x 10(-5) M, respectively. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 7.1 x 10(-7) M and 2.4 x 10(-6) M on BDDE, and 1.0 x 10(-6) M and 3.5 x 10(-6) M on GCE, respectively. The recovery and RSD values obtained for VAR in plasma suggested BDDE electrode to be preferable in comparison with GCE. The accuracy of the voltammetric method was confirmed by the determination of VAR in plasma spiked with Champix (R) tablets and the results were statistically compared with those obtained with Ultra Performance Liquid Chromatography (UPLC) method, as a reference one.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Analytical Chemistry
T1  - Electrochemical Response and Determination of Varenicline at Boron Doped Diamond, Glassy Carbon and Hanging Mercury Electrodes
VL  - 8
IS  - 1
SP  - 133
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1739
ER  - 

@article{
author = "Aleksić, Mara and Radulović, Valentina and Lijeskić, Nikola and Kapetanović, Vera",
year = "2012",
abstract = "The electrochemical study of varenicline (VAR) was done in a wide pH range (2-12), at boron doped diamond electrode (BDDE), glassy carbon electrode (GCE) and hanging mercury electrode (HMDE), using cyclic (CV), square wave (SW) and adsorptive stripping square wave (AdSSW) voltammetric techniques. Depending on the pH and the type of the working electrode the characteristic electrochemical behavior of varenicline was established. The mechanism of the reduction process was suggested. Based on the obtained results, the new electroanalytical method was developed for its determination in the buffer solutions and plasma samples. By applying a square wave voltammetry (SWV) on BDDE and GCE, at pH 3.5 and 4.0, the linear dependence in plasma samples was achieved within the concentration range from 2 10(-6) -1 x 10(-5) M and 4 x 10(-6) -1 x 10(-5) M, respectively. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 7.1 x 10(-7) M and 2.4 x 10(-6) M on BDDE, and 1.0 x 10(-6) M and 3.5 x 10(-6) M on GCE, respectively. The recovery and RSD values obtained for VAR in plasma suggested BDDE electrode to be preferable in comparison with GCE. The accuracy of the voltammetric method was confirmed by the determination of VAR in plasma spiked with Champix (R) tablets and the results were statistically compared with those obtained with Ultra Performance Liquid Chromatography (UPLC) method, as a reference one.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Analytical Chemistry",
title = "Electrochemical Response and Determination of Varenicline at Boron Doped Diamond, Glassy Carbon and Hanging Mercury Electrodes",
volume = "8",
number = "1",
pages = "133-142",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1739"
}

Aleksić, M., Radulović, V., Lijeskić, N.,& Kapetanović, V.. (2012). Electrochemical Response and Determination of Varenicline at Boron Doped Diamond, Glassy Carbon and Hanging Mercury Electrodes. in Current Analytical Chemistry
Bentham Science Publ Ltd, Sharjah., 8(1), 133-142.
https://hdl.handle.net/21.15107/rcub_farfar_1739

Aleksić M, Radulović V, Lijeskić N, Kapetanović V. Electrochemical Response and Determination of Varenicline at Boron Doped Diamond, Glassy Carbon and Hanging Mercury Electrodes. in Current Analytical Chemistry. 2012;8(1):133-142.
https://hdl.handle.net/21.15107/rcub_farfar_1739 .

Aleksić, Mara, Radulović, Valentina, Lijeskić, Nikola, Kapetanović, Vera, "Electrochemical Response and Determination of Varenicline at Boron Doped Diamond, Glassy Carbon and Hanging Mercury Electrodes" in Current Analytical Chemistry, 8, no. 1 (2012):133-142,
https://hdl.handle.net/21.15107/rcub_farfar_1739 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1345
AB  - In the last two decades different electroanalytical methods were used for sensitive and selective determination of cephalosporins. The paper was focused on the electrochemical behavior of methoxyimino cephalosporins, reduction mechanism and nature of the process at the mercury electrode surface. Special attention was paid to the cephalosporins adsorption at the mercury surface. Based on this phenomenon, the adsorptive stripping methods were established for determination of the low concentration of these drugs in urine samples, both in vitro, and in vivo conditions. The application of Adsorptive Stripping Differential Pulse Voltammetry (AdSDPV) for the determination of cefpodoxime proxetil (CP), cefotaxime (CF), desacetylcefotaxime (DCF) and cefetamet (CEF) was summarized. The best sensitivity of in vitro determination in urine was achieved for CP, in acid solutions (LOD 7.4.10(-9) M and LOQ 2.4.10(-8) M), followed by CF, CEF and DCF. This is in accordance with the strength of their adsorption. Determination of CF and DCF by AdSDPV in vivo was also presented. Compared to other analytical methods, AdSDPV showed advantages in the simplicity of sample preparation, and over the other voltamperometric methods, higher sensitivity and selectivity.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Combinatorial Chemistry & High Throughput Screening
T1  - Application of Adsorptive Stripping Voltammetry for the Determination of Selected Methoxyimino Cephalosporins in Urine Samples
VL  - 13
IS  - 8
SP  - 758
EP  - 763
DO  - 10.2174/138620710791920310
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1345
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2010",
abstract = "In the last two decades different electroanalytical methods were used for sensitive and selective determination of cephalosporins. The paper was focused on the electrochemical behavior of methoxyimino cephalosporins, reduction mechanism and nature of the process at the mercury electrode surface. Special attention was paid to the cephalosporins adsorption at the mercury surface. Based on this phenomenon, the adsorptive stripping methods were established for determination of the low concentration of these drugs in urine samples, both in vitro, and in vivo conditions. The application of Adsorptive Stripping Differential Pulse Voltammetry (AdSDPV) for the determination of cefpodoxime proxetil (CP), cefotaxime (CF), desacetylcefotaxime (DCF) and cefetamet (CEF) was summarized. The best sensitivity of in vitro determination in urine was achieved for CP, in acid solutions (LOD 7.4.10(-9) M and LOQ 2.4.10(-8) M), followed by CF, CEF and DCF. This is in accordance with the strength of their adsorption. Determination of CF and DCF by AdSDPV in vivo was also presented. Compared to other analytical methods, AdSDPV showed advantages in the simplicity of sample preparation, and over the other voltamperometric methods, higher sensitivity and selectivity.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Combinatorial Chemistry & High Throughput Screening",
title = "Application of Adsorptive Stripping Voltammetry for the Determination of Selected Methoxyimino Cephalosporins in Urine Samples",
volume = "13",
number = "8",
pages = "758-763",
doi = "10.2174/138620710791920310",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1345"
}

Aleksić, M.,& Kapetanović, V.. (2010). Application of Adsorptive Stripping Voltammetry for the Determination of Selected Methoxyimino Cephalosporins in Urine Samples. in Combinatorial Chemistry & High Throughput Screening
Bentham Science Publ Ltd, Sharjah., 13(8), 758-763.
https://doi.org/10.2174/138620710791920310
https://hdl.handle.net/21.15107/rcub_farfar_1345

Aleksić M, Kapetanović V. Application of Adsorptive Stripping Voltammetry for the Determination of Selected Methoxyimino Cephalosporins in Urine Samples. in Combinatorial Chemistry & High Throughput Screening. 2010;13(8):758-763.
doi:10.2174/138620710791920310
https://hdl.handle.net/21.15107/rcub_farfar_1345 .

Aleksić, Mara, Kapetanović, Vera, "Application of Adsorptive Stripping Voltammetry for the Determination of Selected Methoxyimino Cephalosporins in Urine Samples" in Combinatorial Chemistry & High Throughput Screening, 13, no. 8 (2010):758-763,
https://doi.org/10.2174/138620710791920310 .,
https://hdl.handle.net/21.15107/rcub_farfar_1345 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Radulović, Valentina
AU  - Kapetanović, Vera
AU  - Savić, Vladimir
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1359
AB  - The electrochemical behaviour of desloratadine (DLOR) and its derivative 3-hydroxydesloratadine (3OH-DLOR) was investigated by direct current (DCP) polarography, cyclic (CV), differential pulse (DPV) and square-wave (SWV) voltammetry in Britton-Robinson (BR) buffer solutions (pH 4-11). Both compounds are reduced at mercury electrode in irreversible two electron reduction of the C=N bond of the pyridine ring in their molecules. The difference in their electrochemical behaviour was investigated, and the most pronounced distinction is observed at pH > 9, as a consequence of the deprotonation of the phenolic moiety in 3OH-DLOR molecule, yielding significant change in their reduction potentials (E (p DLOR) = -1.48 V, and E(p 3OH-DLOR) = -1.6 V). The observed results correlate with calculated LUMO energy levels and Hammet substituent constants (sigma). Based on the difference in the reduction potential for DLOR and 3OH-DLOR, conditions for simultaneous determination these two molecules in alkaline medium were established. The best selectivity was achieved using SWV method at pH 10. The linearity of the calibration graphs were achieved in the concentration range from 1.5 x 10(-6) M-1 x 10(-5) M for DLOR and 7.5 x 10(-6) M(-5) x 10(-5) M for 3OH-DLOR with detection limits of 2.29 x 10(-7) M and 2.08 x 10(-6) M, and determination limits of 7.64 x 10(-7) M and 6.94 x 10(-6) M, for DLOR and 3OH-DLOR, respectively. The method was checked in human plasma sample. Good response was obtained with LOD and LOQ values of 4.63 x 10(-7) M and 1.54 x 10(-6) M, for DLOR and 2.39 x 10(-6) M and 7.97 x 10(-6) M, 3OH-DLOR, respectively.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - The Possibility of Simultaneous Voltammetric Determination of Desloratadine and 3-Hydroxydesloratadine
VL  - 57
IS  - 3
SP  - 686
EP  - 692
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1359
ER  - 

@article{
author = "Aleksić, Mara and Radulović, Valentina and Kapetanović, Vera and Savić, Vladimir",
year = "2010",
abstract = "The electrochemical behaviour of desloratadine (DLOR) and its derivative 3-hydroxydesloratadine (3OH-DLOR) was investigated by direct current (DCP) polarography, cyclic (CV), differential pulse (DPV) and square-wave (SWV) voltammetry in Britton-Robinson (BR) buffer solutions (pH 4-11). Both compounds are reduced at mercury electrode in irreversible two electron reduction of the C=N bond of the pyridine ring in their molecules. The difference in their electrochemical behaviour was investigated, and the most pronounced distinction is observed at pH > 9, as a consequence of the deprotonation of the phenolic moiety in 3OH-DLOR molecule, yielding significant change in their reduction potentials (E (p DLOR) = -1.48 V, and E(p 3OH-DLOR) = -1.6 V). The observed results correlate with calculated LUMO energy levels and Hammet substituent constants (sigma). Based on the difference in the reduction potential for DLOR and 3OH-DLOR, conditions for simultaneous determination these two molecules in alkaline medium were established. The best selectivity was achieved using SWV method at pH 10. The linearity of the calibration graphs were achieved in the concentration range from 1.5 x 10(-6) M-1 x 10(-5) M for DLOR and 7.5 x 10(-6) M(-5) x 10(-5) M for 3OH-DLOR with detection limits of 2.29 x 10(-7) M and 2.08 x 10(-6) M, and determination limits of 7.64 x 10(-7) M and 6.94 x 10(-6) M, for DLOR and 3OH-DLOR, respectively. The method was checked in human plasma sample. Good response was obtained with LOD and LOQ values of 4.63 x 10(-7) M and 1.54 x 10(-6) M, for DLOR and 2.39 x 10(-6) M and 7.97 x 10(-6) M, 3OH-DLOR, respectively.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "The Possibility of Simultaneous Voltammetric Determination of Desloratadine and 3-Hydroxydesloratadine",
volume = "57",
number = "3",
pages = "686-692",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1359"
}

Aleksić, M., Radulović, V., Kapetanović, V.,& Savić, V.. (2010). The Possibility of Simultaneous Voltammetric Determination of Desloratadine and 3-Hydroxydesloratadine. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 57(3), 686-692.
https://hdl.handle.net/21.15107/rcub_farfar_1359

Aleksić M, Radulović V, Kapetanović V, Savić V. The Possibility of Simultaneous Voltammetric Determination of Desloratadine and 3-Hydroxydesloratadine. in Acta Chimica Slovenica. 2010;57(3):686-692.
https://hdl.handle.net/21.15107/rcub_farfar_1359 .

Aleksić, Mara, Radulović, Valentina, Kapetanović, Vera, Savić, Vladimir, "The Possibility of Simultaneous Voltammetric Determination of Desloratadine and 3-Hydroxydesloratadine" in Acta Chimica Slovenica, 57, no. 3 (2010):686-692,
https://hdl.handle.net/21.15107/rcub_farfar_1359 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1310
AB  - In last two decades different electroanalytical methods are used for sensitive and selective determination of cephalosporins. In this paper the electrochemical behavior of methoxyimino cephalosporins, reduction mechanism and nature of the process at the mercury electrode surface is presented. Special attention is paid to the cephalosporin ability to adsorb on the electrode surface. Based on the methoxyimino cephalosporin specific adsorption on the mercury surface, the adsorptive stripping methods are established for determination of low concentration of these drugs in urine samples, both in-vitro, and in-vivo. Application of the adsorptive stripping differential pulse voltammetry (AdSDPV) for determination of cefpodoxime proksetile (CP), cefotaxime (CF), desacetylcefotaxime (DCF) and cefetamet (CEF) is summarized. The best sensitivity of in-vitro determination in urine was achieved for CP in acid solutions (LOD 7.4×10-9M and LOQ 2.4×10-8M), what is in accordance with its most pronounced adsorption properties, and followed by CF, DCF and CEF. In-vivo AdSDPV determination of CF and DCF is also presented. AdSDPV showed advantages over other analytical methods in simplicity of the sample preparation, and is even more sensitive and selective compared to other voltamperometric methods.
AB  - U poslednje dve dekade elektroanalitičke metode su primenjivane za osetljiva i selektivna određivanja velikog broja cefalosporina. U ovom radu detaljno je prikazano elektrohemijsko ponašanje metoksiimino cefalosporina, mehanizam i priroda procesa njihove redukcije na površini živine elektrode, a posebna pažnja posvećena je sposobnosti molekula cefalosporina da se adsorbuju na površini elektrode. Voltametrijske metode sa akumulacijom zasnivaju se na procesu specifične adsorpcije cefalosporina na površini živine elektrode i primenjuju se za određivanje veoma niskih koncentracija cefalosporina kako in-vitro, tako i in-vivo u biološkom materijalu urinu. Sumirani su rezultati primene metode adsorptivne 'striping' diferencijalno pulsne voltametrije (AdSDPV) za određivanje cefpodoksim proksetila (CP), cefotaksima (CF), dezacetilcefotaksima (DCF) i cefetameta (CEF). Najveća osetljivost in-vitro određivanja u uzorcima urina, ostvarena je za CP u kiseloj sredini (granica detekcije: 7,4×10-9M i granica određivanja: 2,4×10-8M), što je u saglasnosti sa najizraženijom adsorpcijom ovog antibiotika, a zatim slede CF, DCF i na kraju CEF. Prikazana je i primena AdSDPV metode za in-vivo određivanja CF i DCF. Prednost AdSDPV nad drugim analitičkim metodama je u jednostavnosti pripreme uzorka, a prednost nad ostalim voltamperometrijskim metodama leži u većoj osetljivosti i selektivnosti.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Electrochemical behavior of methoxyimino cephalosporins and their in-vitro and in-vivo determination in urine by adsorptive stripping voltammetry
T1  - Elektrohemijsko ponašanje metoksiimino cefalosporina i njihovo in-vitro i in-vivo određivanje u urinu primenom adsorptivne 'striping' voltametrije
VL  - 59
IS  - 6
SP  - 509
EP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1310
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2009",
abstract = "In last two decades different electroanalytical methods are used for sensitive and selective determination of cephalosporins. In this paper the electrochemical behavior of methoxyimino cephalosporins, reduction mechanism and nature of the process at the mercury electrode surface is presented. Special attention is paid to the cephalosporin ability to adsorb on the electrode surface. Based on the methoxyimino cephalosporin specific adsorption on the mercury surface, the adsorptive stripping methods are established for determination of low concentration of these drugs in urine samples, both in-vitro, and in-vivo. Application of the adsorptive stripping differential pulse voltammetry (AdSDPV) for determination of cefpodoxime proksetile (CP), cefotaxime (CF), desacetylcefotaxime (DCF) and cefetamet (CEF) is summarized. The best sensitivity of in-vitro determination in urine was achieved for CP in acid solutions (LOD 7.4×10-9M and LOQ 2.4×10-8M), what is in accordance with its most pronounced adsorption properties, and followed by CF, DCF and CEF. In-vivo AdSDPV determination of CF and DCF is also presented. AdSDPV showed advantages over other analytical methods in simplicity of the sample preparation, and is even more sensitive and selective compared to other voltamperometric methods., U poslednje dve dekade elektroanalitičke metode su primenjivane za osetljiva i selektivna određivanja velikog broja cefalosporina. U ovom radu detaljno je prikazano elektrohemijsko ponašanje metoksiimino cefalosporina, mehanizam i priroda procesa njihove redukcije na površini živine elektrode, a posebna pažnja posvećena je sposobnosti molekula cefalosporina da se adsorbuju na površini elektrode. Voltametrijske metode sa akumulacijom zasnivaju se na procesu specifične adsorpcije cefalosporina na površini živine elektrode i primenjuju se za određivanje veoma niskih koncentracija cefalosporina kako in-vitro, tako i in-vivo u biološkom materijalu urinu. Sumirani su rezultati primene metode adsorptivne 'striping' diferencijalno pulsne voltametrije (AdSDPV) za određivanje cefpodoksim proksetila (CP), cefotaksima (CF), dezacetilcefotaksima (DCF) i cefetameta (CEF). Najveća osetljivost in-vitro određivanja u uzorcima urina, ostvarena je za CP u kiseloj sredini (granica detekcije: 7,4×10-9M i granica određivanja: 2,4×10-8M), što je u saglasnosti sa najizraženijom adsorpcijom ovog antibiotika, a zatim slede CF, DCF i na kraju CEF. Prikazana je i primena AdSDPV metode za in-vivo određivanja CF i DCF. Prednost AdSDPV nad drugim analitičkim metodama je u jednostavnosti pripreme uzorka, a prednost nad ostalim voltamperometrijskim metodama leži u većoj osetljivosti i selektivnosti.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Electrochemical behavior of methoxyimino cephalosporins and their in-vitro and in-vivo determination in urine by adsorptive stripping voltammetry, Elektrohemijsko ponašanje metoksiimino cefalosporina i njihovo in-vitro i in-vivo određivanje u urinu primenom adsorptivne 'striping' voltametrije",
volume = "59",
number = "6",
pages = "509-523",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1310"
}

Aleksić, M.,& Kapetanović, V.. (2009). Electrochemical behavior of methoxyimino cephalosporins and their in-vitro and in-vivo determination in urine by adsorptive stripping voltammetry. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 59(6), 509-523.
https://hdl.handle.net/21.15107/rcub_farfar_1310

Aleksić M, Kapetanović V. Electrochemical behavior of methoxyimino cephalosporins and their in-vitro and in-vivo determination in urine by adsorptive stripping voltammetry. in Arhiv za farmaciju. 2009;59(6):509-523.
https://hdl.handle.net/21.15107/rcub_farfar_1310 .

Aleksić, Mara, Kapetanović, Vera, "Electrochemical behavior of methoxyimino cephalosporins and their in-vitro and in-vivo determination in urine by adsorptive stripping voltammetry" in Arhiv za farmaciju, 59, no. 6 (2009):509-523,
https://hdl.handle.net/21.15107/rcub_farfar_1310 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
AU  - Atanacković, Jasmina
AU  - Jocić, Biljana
AU  - Zečević, Mira
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1032
AB  - Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.
PB  - Elsevier Science BV, Amsterdam
T2  - Talanta
T1  - Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods
VL  - 77
IS  - 1
SP  - 131
EP  - 137
DO  - 10.1016/j.talanta.2008.05.047
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera and Atanacković, Jasmina and Jocić, Biljana and Zečević, Mira",
year = "2008",
abstract = "Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods",
volume = "77",
number = "1",
pages = "131-137",
doi = "10.1016/j.talanta.2008.05.047"
}

Aleksić, M., Kapetanović, V., Atanacković, J., Jocić, B.,& Zečević, M.. (2008). Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta
Elsevier Science BV, Amsterdam., 77(1), 131-137.
https://doi.org/10.1016/j.talanta.2008.05.047

Aleksić M, Kapetanović V, Atanacković J, Jocić B, Zečević M. Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta. 2008;77(1):131-137.
doi:10.1016/j.talanta.2008.05.047 .

Aleksić, Mara, Kapetanović, Vera, Atanacković, Jasmina, Jocić, Biljana, Zečević, Mira, "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods" in Talanta, 77, no. 1 (2008):131-137,
https://doi.org/10.1016/j.talanta.2008.05.047 . .

TY  - JOUR
AU  - Ilić, Miloš
AU  - Atanacković, Jasmina
AU  - Kapetanović, Vera
AU  - Aleksić, Mara
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/911
AB  - There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC.
AB  - Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Analytical methods for determination of cephalosporins in cerebrospinal fluid
T1  - Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti
VL  - 57
IS  - 3
SP  - 164
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_farfar_911
ER  - 

@article{
author = "Ilić, Miloš and Atanacković, Jasmina and Kapetanović, Vera and Aleksić, Mara",
year = "2007",
abstract = "There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC., Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Analytical methods for determination of cephalosporins in cerebrospinal fluid, Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti",
volume = "57",
number = "3",
pages = "164-177",
url = "https://hdl.handle.net/21.15107/rcub_farfar_911"
}

Ilić, M., Atanacković, J., Kapetanović, V.,& Aleksić, M.. (2007). Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 57(3), 164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911

Ilić M, Atanacković J, Kapetanović V, Aleksić M. Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju. 2007;57(3):164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911 .

Ilić, Miloš, Atanacković, Jasmina, Kapetanović, Vera, Aleksić, Mara, "Analytical methods for determination of cephalosporins in cerebrospinal fluid" in Arhiv za farmaciju, 57, no. 3 (2007):164-177,
https://hdl.handle.net/21.15107/rcub_farfar_911 .

TY  - JOUR
AU  - Aleksić, Mara M.
AU  - Kapetanović, Vera
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5468
AB  - Abstract
The voltammetric behavior of cefotaxime (CFX) is investigated by cyclic and square-wave voltammetry in BR buffers (pH 1.80–12.0).
Based on the cathodic reduction peak at approximately 0.5 V in BR buffer (pH 2.8 and 9.25), a robust, highly reliable square-wave
voltammetric method (SW) was developed for determination of CFX. The linearity was achieved by SW voltammetry in 7.5 · 109 M
(3.52 ng ml1
) to 1.0 · 107 M (47 ng ml1
) and from 1.2 · 107 M (56.4 ng ml1
) to 1.2 · 106 M (568.8 ng ml1
) range at pH 2.8 with
detection and quantification limits of 0.813 ng ml1 and 2.71 ng ml1
, and 12.6 ng ml1 and 42.2 ng ml1
, respectively. At pH 9.25 linear
range was obtained from 4 · 108 M (18.8 ng ml1
) to 1.6 · 107 M (75.2 ng ml1
) with detection and quantification limits of
2.947 ng ml1 and 9.82 ng ml1
. The method was applied for CFX determination in spiked humane urine sample. A detection and quantification limits of 7.2 ng ml1 and 23.9 ng ml1 were achieved for determination of CFX in urine at pH 2.8 and 13.6 ng ml1 and
45.1 ng ml1 at pH 9.25. The comparative reference method was DPV. The proposed method was checked for determination of CFX
in real human urine, and selectivity of the method over the metabolites was found to be quite satisfactory.
PB  - Elsevier
T2  - Journal of Electroanalytical Chemistry
T1  - Voltammetric behavior and square-wave voltammetric determination of cefotaxime in urine
VL  - 593
IS  - 1-2
SP  - 258
EP  - 266
DO  - 10.1016/j.jelechem.2006.06.011
ER  - 

@article{
author = "Aleksić, Mara M. and Kapetanović, Vera",
year = "2006",
abstract = "Abstract
The voltammetric behavior of cefotaxime (CFX) is investigated by cyclic and square-wave voltammetry in BR buffers (pH 1.80–12.0).
Based on the cathodic reduction peak at approximately 0.5 V in BR buffer (pH 2.8 and 9.25), a robust, highly reliable square-wave
voltammetric method (SW) was developed for determination of CFX. The linearity was achieved by SW voltammetry in 7.5 · 109 M
(3.52 ng ml1
) to 1.0 · 107 M (47 ng ml1
) and from 1.2 · 107 M (56.4 ng ml1
) to 1.2 · 106 M (568.8 ng ml1
) range at pH 2.8 with
detection and quantification limits of 0.813 ng ml1 and 2.71 ng ml1
, and 12.6 ng ml1 and 42.2 ng ml1
, respectively. At pH 9.25 linear
range was obtained from 4 · 108 M (18.8 ng ml1
) to 1.6 · 107 M (75.2 ng ml1
) with detection and quantification limits of
2.947 ng ml1 and 9.82 ng ml1
. The method was applied for CFX determination in spiked humane urine sample. A detection and quantification limits of 7.2 ng ml1 and 23.9 ng ml1 were achieved for determination of CFX in urine at pH 2.8 and 13.6 ng ml1 and
45.1 ng ml1 at pH 9.25. The comparative reference method was DPV. The proposed method was checked for determination of CFX
in real human urine, and selectivity of the method over the metabolites was found to be quite satisfactory.",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Voltammetric behavior and square-wave voltammetric determination of cefotaxime in urine",
volume = "593",
number = "1-2",
pages = "258-266",
doi = "10.1016/j.jelechem.2006.06.011"
}

Aleksić, M. M.,& Kapetanović, V.. (2006). Voltammetric behavior and square-wave voltammetric determination of cefotaxime in urine. in Journal of Electroanalytical Chemistry
Elsevier., 593(1-2), 258-266.
https://doi.org/10.1016/j.jelechem.2006.06.011

Aleksić MM, Kapetanović V. Voltammetric behavior and square-wave voltammetric determination of cefotaxime in urine. in Journal of Electroanalytical Chemistry. 2006;593(1-2):258-266.
doi:10.1016/j.jelechem.2006.06.011 .

Aleksić, Mara M., Kapetanović, Vera, "Voltammetric behavior and square-wave voltammetric determination of cefotaxime in urine" in Journal of Electroanalytical Chemistry, 593, no. 1-2 (2006):258-266,
https://doi.org/10.1016/j.jelechem.2006.06.011 . .

TY  - JOUR
AU  - Brborić, Jasmina
AU  - Jovanović, Mirjana S.
AU  - Popović, Gordana
AU  - Kapetanović, Vera
AU  - Vladimirov, Sote
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/875
AB  - The acid-base equilibria of a novel hepatobiliary imaging agent, 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid (DIIODIDA) were studied. The potentiometrically determined acidity constants of the second carboxylic group, amino and amide groups were pK2 = 2.52 ± 0.02; pK3 = 5.86 ± 0.06 and pK4 = 10.9 ± 0.1. The determinations were performed at 25 ºC and an ionic strength of 0.1 mol/dm3 (NaCl). The acidity constants (pK1 = 1.3 ± 0.4) corresponding to the first carboxylic group was determined indirectly, on the basis of equilibrium constants obtained in a heterogeneous system, at 25 ºC and an ionic strength 1 mol/dm3 (HCl, NaCl). DIIODIDA was labeled with technetium-99m, and the influence of pH on the yield of labeling was investigated. It was found that labeling within the pH range from 5.5 to 6.5 provided a radiopharmaceutical of high radiochemical purity (>98 %).
AB  - Ispitane su kiselinsko-bazne ravnoteže novog hepatobilijarnog agensa, 2,4-dijod- 6-metilfenilkarbamoilmetil iminodisirćetne kiseline. Kiselinske konstante koje odgovaraju drugoj karboksilnoj grupi, amino i amidnoj grupi određene su potenciometrijski: pK2 = 2.52 ± 0.02; pK3 = 5.86 ± 0.06 I pK4 = 10.9 ± 0.1. Određivanja su izvedena na 25 ºC i pri konstantnoj jonskoj sili 0,1 mol/dm3 (NaCl). Kiselinska konstanta prve karboksilne grupe (pK1 = 1,3_0,4) određena je indirektno na osnovu ravnotežnih konstanti određenih u heterogenom sistemu, na 25 ºC i jonskoj sili 1 mol/dm3 (HCl, NaCl). DIIODIDA je obeležena tehnecijumom-99m i ispitan je uticaj pH na prinos obeležavanja. Utvrđeno je da se obeležavanjem u pH opsegu 5,5-6,5 dobija radiofarmaceutik visoke radiohemijske čistoće (> 98 %).
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Acid-base equilibria of 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid and its labeling with technetium-99m
T1  - Kiselinsko-bazne ravnoteže 2,4-dijod-6-metilfenilkarbamoilmetil iminodisirćetne kiseline i obeležavanje tehnecijumom-99m
VL  - 71
IS  - 1
SP  - 55
EP  - 65
DO  - 10.2298/JSC0601055B
ER  - 

@article{
author = "Brborić, Jasmina and Jovanović, Mirjana S. and Popović, Gordana and Kapetanović, Vera and Vladimirov, Sote",
year = "2006",
abstract = "The acid-base equilibria of a novel hepatobiliary imaging agent, 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid (DIIODIDA) were studied. The potentiometrically determined acidity constants of the second carboxylic group, amino and amide groups were pK2 = 2.52 ± 0.02; pK3 = 5.86 ± 0.06 and pK4 = 10.9 ± 0.1. The determinations were performed at 25 ºC and an ionic strength of 0.1 mol/dm3 (NaCl). The acidity constants (pK1 = 1.3 ± 0.4) corresponding to the first carboxylic group was determined indirectly, on the basis of equilibrium constants obtained in a heterogeneous system, at 25 ºC and an ionic strength 1 mol/dm3 (HCl, NaCl). DIIODIDA was labeled with technetium-99m, and the influence of pH on the yield of labeling was investigated. It was found that labeling within the pH range from 5.5 to 6.5 provided a radiopharmaceutical of high radiochemical purity (>98 %)., Ispitane su kiselinsko-bazne ravnoteže novog hepatobilijarnog agensa, 2,4-dijod- 6-metilfenilkarbamoilmetil iminodisirćetne kiseline. Kiselinske konstante koje odgovaraju drugoj karboksilnoj grupi, amino i amidnoj grupi određene su potenciometrijski: pK2 = 2.52 ± 0.02; pK3 = 5.86 ± 0.06 I pK4 = 10.9 ± 0.1. Određivanja su izvedena na 25 ºC i pri konstantnoj jonskoj sili 0,1 mol/dm3 (NaCl). Kiselinska konstanta prve karboksilne grupe (pK1 = 1,3_0,4) određena je indirektno na osnovu ravnotežnih konstanti određenih u heterogenom sistemu, na 25 ºC i jonskoj sili 1 mol/dm3 (HCl, NaCl). DIIODIDA je obeležena tehnecijumom-99m i ispitan je uticaj pH na prinos obeležavanja. Utvrđeno je da se obeležavanjem u pH opsegu 5,5-6,5 dobija radiofarmaceutik visoke radiohemijske čistoće (> 98 %).",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Acid-base equilibria of 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid and its labeling with technetium-99m, Kiselinsko-bazne ravnoteže 2,4-dijod-6-metilfenilkarbamoilmetil iminodisirćetne kiseline i obeležavanje tehnecijumom-99m",
volume = "71",
number = "1",
pages = "55-65",
doi = "10.2298/JSC0601055B"
}

Brborić, J., Jovanović, M. S., Popović, G., Kapetanović, V.,& Vladimirov, S.. (2006). Acid-base equilibria of 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid and its labeling with technetium-99m. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(1), 55-65.
https://doi.org/10.2298/JSC0601055B

Brborić J, Jovanović MS, Popović G, Kapetanović V, Vladimirov S. Acid-base equilibria of 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid and its labeling with technetium-99m. in Journal of the Serbian Chemical Society. 2006;71(1):55-65.
doi:10.2298/JSC0601055B .

Brborić, Jasmina, Jovanović, Mirjana S., Popović, Gordana, Kapetanović, Vera, Vladimirov, Sote, "Acid-base equilibria of 2,4-diiodo-6-methylphenylcarbamoylmethyl iminodiacetic acid and its labeling with technetium-99m" in Journal of the Serbian Chemical Society, 71, no. 1 (2006):55-65,
https://doi.org/10.2298/JSC0601055B . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Savić, Vladimir
AU  - Popović, Gordana
AU  - Burić, Nikola
AU  - Kapetanović, Vera
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/616
AB  - Ionization constants of three cephalosporin antibiotics, cefetamet (CEF), cefotaxime (CFX) and ceftriaxone (CFTR) are determined using pH-potentiometric titrations at I= 0.1 M (NaCl) and t = 25 degrees C. Cefetarnet and cefotaxime have three ionization groups: carboxylic, amide and aminothiazole. Besides those three, ceftriaxone possesses an hydroxytriazi none group as new and additional ionization center. In acid medium two overlapping acid-base processes are occuring with acidity constants being: pK(1) 2.93 (COOH) and PK2 3.07 (amin nothiazole) for cefetamet, and pK(1) 2.21 (COOH) and pK(2) 3.15 (aminothiazole) for cefotaxime. In the case of ceftriaxone the situation is even more complicated, three overlapping processes coexist with pK(1) 2.37 (COOH), pK(2) 3.03 (aminothiazole) and pK(3) 4.21 (hydroxytriazinone). Protolysis of amide group is happening in the alkaline medium as completely separated process from those in acid medium. The acidity constants which correspond to amide group are pK(3) 10.65 (CEF), pK(3) 10.87 (CFX) and pK(4) 10.74 (CFTR). The influence of the C3 substituent on the dissociation process of the neighboring ionization group, particularly carboxylic group, was considered. The differences in acidity of CEF, CFX and CFFR pK(1): 2.93, 2.21 and 2.37, respectively) are likely to be caused by the stereoelectronic properties of substituents in the P-position to the carboxylic group due to the combined inductive, hyperconjugative and resonance effects.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Acidity constants of cefetamet, cefotaxime and ceftriaxone; the effect of the substituent at C3 position
VL  - 39
IS  - 3-4
SP  - 752
EP  - 756
DO  - 10.1016/j.jpba.2005.04.033
ER  - 

@article{
author = "Aleksić, Mara and Savić, Vladimir and Popović, Gordana and Burić, Nikola and Kapetanović, Vera",
year = "2005",
abstract = "Ionization constants of three cephalosporin antibiotics, cefetamet (CEF), cefotaxime (CFX) and ceftriaxone (CFTR) are determined using pH-potentiometric titrations at I= 0.1 M (NaCl) and t = 25 degrees C. Cefetarnet and cefotaxime have three ionization groups: carboxylic, amide and aminothiazole. Besides those three, ceftriaxone possesses an hydroxytriazi none group as new and additional ionization center. In acid medium two overlapping acid-base processes are occuring with acidity constants being: pK(1) 2.93 (COOH) and PK2 3.07 (amin nothiazole) for cefetamet, and pK(1) 2.21 (COOH) and pK(2) 3.15 (aminothiazole) for cefotaxime. In the case of ceftriaxone the situation is even more complicated, three overlapping processes coexist with pK(1) 2.37 (COOH), pK(2) 3.03 (aminothiazole) and pK(3) 4.21 (hydroxytriazinone). Protolysis of amide group is happening in the alkaline medium as completely separated process from those in acid medium. The acidity constants which correspond to amide group are pK(3) 10.65 (CEF), pK(3) 10.87 (CFX) and pK(4) 10.74 (CFTR). The influence of the C3 substituent on the dissociation process of the neighboring ionization group, particularly carboxylic group, was considered. The differences in acidity of CEF, CFX and CFFR pK(1): 2.93, 2.21 and 2.37, respectively) are likely to be caused by the stereoelectronic properties of substituents in the P-position to the carboxylic group due to the combined inductive, hyperconjugative and resonance effects.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Acidity constants of cefetamet, cefotaxime and ceftriaxone; the effect of the substituent at C3 position",
volume = "39",
number = "3-4",
pages = "752-756",
doi = "10.1016/j.jpba.2005.04.033"
}

Aleksić, M., Savić, V., Popović, G., Burić, N.,& Kapetanović, V.. (2005). Acidity constants of cefetamet, cefotaxime and ceftriaxone; the effect of the substituent at C3 position. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 39(3-4), 752-756.
https://doi.org/10.1016/j.jpba.2005.04.033

Aleksić M, Savić V, Popović G, Burić N, Kapetanović V. Acidity constants of cefetamet, cefotaxime and ceftriaxone; the effect of the substituent at C3 position. in Journal of Pharmaceutical and Biomedical Analysis. 2005;39(3-4):752-756.
doi:10.1016/j.jpba.2005.04.033 .

Aleksić, Mara, Savić, Vladimir, Popović, Gordana, Burić, Nikola, Kapetanović, Vera, "Acidity constants of cefetamet, cefotaxime and ceftriaxone; the effect of the substituent at C3 position" in Journal of Pharmaceutical and Biomedical Analysis, 39, no. 3-4 (2005):752-756,
https://doi.org/10.1016/j.jpba.2005.04.033 . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Ilić, Miloš
AU  - Kapetanović, Vera
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5467
AB  - Abstract
It was found that the reduction of the cefpodoxime proxetil (CP) molecule is strongly influenced by the adsorption. The adsorptive
properties of CP were investigated in order to achieve an increase sensitivity of its determination. Validated adsorptive stripping differential
pulse voltammetry is applied for the determination of low concentration of CP at pH 3.5 and 9.0 where the best pronounced adsorption effects
were observed. The linearity of the calibration curves were achieved from 1 × 10−8 to 1 × 10−7 M with limit of detection (LOD) of 6.3×10−9
and 7.1 × 10−9 M, and limit of quantification (LOQ) of 2.1 × 10−8 and 2.3 × 10−8 M, at pH 3.5 and 9.0, respectively. The proposed method
was tested for CP determination in spiked urine samples, enabling determination of low concentrations of CP.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical  Analysis
T1  - Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine
VL  - 36
IS  - 4
SP  - 899
EP  - 993
DO  - 10.1016/j.jpba.2004.08.035
ER  - 

@article{
author = "Aleksić, Mara and Ilić, Miloš and Kapetanović, Vera",
year = "2004",
abstract = "Abstract
It was found that the reduction of the cefpodoxime proxetil (CP) molecule is strongly influenced by the adsorption. The adsorptive
properties of CP were investigated in order to achieve an increase sensitivity of its determination. Validated adsorptive stripping differential
pulse voltammetry is applied for the determination of low concentration of CP at pH 3.5 and 9.0 where the best pronounced adsorption effects
were observed. The linearity of the calibration curves were achieved from 1 × 10−8 to 1 × 10−7 M with limit of detection (LOD) of 6.3×10−9
and 7.1 × 10−9 M, and limit of quantification (LOQ) of 2.1 × 10−8 and 2.3 × 10−8 M, at pH 3.5 and 9.0, respectively. The proposed method
was tested for CP determination in spiked urine samples, enabling determination of low concentrations of CP.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical  Analysis",
title = "Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine",
volume = "36",
number = "4",
pages = "899-993",
doi = "10.1016/j.jpba.2004.08.035"
}

Aleksić, M., Ilić, M.,& Kapetanović, V.. (2004). Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine. in Journal of Pharmaceutical and Biomedical  Analysis
Elsevier B.V.., 36(4), 899-993.
https://doi.org/10.1016/j.jpba.2004.08.035

Aleksić M, Ilić M, Kapetanović V. Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine. in Journal of Pharmaceutical and Biomedical  Analysis. 2004;36(4):899-993.
doi:10.1016/j.jpba.2004.08.035 .

Aleksić, Mara, Ilić, Miloš, Kapetanović, Vera, "Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine" in Journal of Pharmaceutical and Biomedical  Analysis, 36, no. 4 (2004):899-993,
https://doi.org/10.1016/j.jpba.2004.08.035 . .

TY  - JOUR
AU  - Jovanović, MS
AU  - Popović, Gordana
AU  - Kapetanović, Vera
AU  - Orlić, M
AU  - Vladimirov, S
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/516
AB  - In order to develop a radiopharmaceutical for hepatobiliary scintigraphy with better hepatobiliary properties new ligand for complexation of Tc-99m, 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid (METHYLIODIDA), was synthesized. Acid-base equilibria of METHYLIODIDA were studied potentiometrically, because these data are important for determination of complex formation conditions. It was established that METHYLIODIDA undergoes a complex acid-base equilibrium due to its zwitterionic nature and four proton-binding sites. The stoichiometric ionization constants were determined at 25 degreesC and constant ionic strength 0.1 M (NaClO4): pK(1) = 1.7 +/- 0.1; pK(2) = 2.44 +/- 0.07; pK(3) = 6.29 +/- 0.02 and pK(4) = 10.91 +/- 0.06, respectively.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Determination of the ionization constants of 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid
VL  - 35
IS  - 5
SP  - 1257
EP  - 1261
DO  - 10.1016/j.jpba.2004.03.009
ER  - 

@article{
author = "Jovanović, MS and Popović, Gordana and Kapetanović, Vera and Orlić, M and Vladimirov, S",
year = "2004",
abstract = "In order to develop a radiopharmaceutical for hepatobiliary scintigraphy with better hepatobiliary properties new ligand for complexation of Tc-99m, 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid (METHYLIODIDA), was synthesized. Acid-base equilibria of METHYLIODIDA were studied potentiometrically, because these data are important for determination of complex formation conditions. It was established that METHYLIODIDA undergoes a complex acid-base equilibrium due to its zwitterionic nature and four proton-binding sites. The stoichiometric ionization constants were determined at 25 degreesC and constant ionic strength 0.1 M (NaClO4): pK(1) = 1.7 +/- 0.1; pK(2) = 2.44 +/- 0.07; pK(3) = 6.29 +/- 0.02 and pK(4) = 10.91 +/- 0.06, respectively.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Determination of the ionization constants of 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid",
volume = "35",
number = "5",
pages = "1257-1261",
doi = "10.1016/j.jpba.2004.03.009"
}

Jovanović, M., Popović, G., Kapetanović, V., Orlić, M.,& Vladimirov, S.. (2004). Determination of the ionization constants of 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 35(5), 1257-1261.
https://doi.org/10.1016/j.jpba.2004.03.009

Jovanović M, Popović G, Kapetanović V, Orlić M, Vladimirov S. Determination of the ionization constants of 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid. in Journal of Pharmaceutical and Biomedical Analysis. 2004;35(5):1257-1261.
doi:10.1016/j.jpba.2004.03.009 .

Jovanović, MS, Popović, Gordana, Kapetanović, Vera, Orlić, M, Vladimirov, S, "Determination of the ionization constants of 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid" in Journal of Pharmaceutical and Biomedical Analysis, 35, no. 5 (2004):1257-1261,
https://doi.org/10.1016/j.jpba.2004.03.009 . .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Milovanović, Ljiljana
AU  - Kapetanović, Vera
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5466
AB  - Abstract
On the basis of previously established mechanism of cefetamet (CEF) reduction, two methods were suggested for
CEF determination*/differential pulse polarographic and differential pulse adsorptive stripping voltammetric method.
Two pH values were chosen, 2.0 and 8.4, where the electrochemical process was defined as one four-electron and two
two-electron processes, respectively. The methods were performed in Britton/Robinson (BR) buffer and the
corresponding calibration graphs were constructed and statistical parameters were evaluated. Applying the AdSV
method at pH 2.0 linearity was achieved from 210 8 to 210 7 M with limit detection and limit determination of
410 9 and 1.410 8 M, respectively. At pH 8.4, the linearity was obtained between 610 8 and 610 7 M,
with limit detection and limit determination of 1.510 8 and 510 8 M, respectively. Since the AdSV method
enabled lower concentrations of CEF to be determined, this method was tested for CEF determination in spiked urine
samples, and DPP method was used as a comparative one.
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical  Analysis
T1  - Adsorptive stripping voltammetric determination of cefetamet in human urine
VL  - 32
IS  - 4-5
SP  - 957
EP  - 966
DO  - 10.1016/S0731-7085(03)00197-3
ER  - 

@article{
author = "Aleksić, Mara and Milovanović, Ljiljana and Kapetanović, Vera",
year = "2003",
abstract = "Abstract
On the basis of previously established mechanism of cefetamet (CEF) reduction, two methods were suggested for
CEF determination*/differential pulse polarographic and differential pulse adsorptive stripping voltammetric method.
Two pH values were chosen, 2.0 and 8.4, where the electrochemical process was defined as one four-electron and two
two-electron processes, respectively. The methods were performed in Britton/Robinson (BR) buffer and the
corresponding calibration graphs were constructed and statistical parameters were evaluated. Applying the AdSV
method at pH 2.0 linearity was achieved from 210 8 to 210 7 M with limit detection and limit determination of
410 9 and 1.410 8 M, respectively. At pH 8.4, the linearity was obtained between 610 8 and 610 7 M,
with limit detection and limit determination of 1.510 8 and 510 8 M, respectively. Since the AdSV method
enabled lower concentrations of CEF to be determined, this method was tested for CEF determination in spiked urine
samples, and DPP method was used as a comparative one.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical  Analysis",
title = "Adsorptive stripping voltammetric determination of cefetamet in human urine",
volume = "32",
number = "4-5",
pages = "957-966",
doi = "10.1016/S0731-7085(03)00197-3"
}

Aleksić, M., Milovanović, L.,& Kapetanović, V.. (2003). Adsorptive stripping voltammetric determination of cefetamet in human urine. in Journal of Pharmaceutical and Biomedical  Analysis
Elsevier., 32(4-5), 957-966.
https://doi.org/10.1016/S0731-7085(03)00197-3

Aleksić M, Milovanović L, Kapetanović V. Adsorptive stripping voltammetric determination of cefetamet in human urine. in Journal of Pharmaceutical and Biomedical  Analysis. 2003;32(4-5):957-966.
doi:10.1016/S0731-7085(03)00197-3 .

Aleksić, Mara, Milovanović, Ljiljana, Kapetanović, Vera, "Adsorptive stripping voltammetric determination of cefetamet in human urine" in Journal of Pharmaceutical and Biomedical  Analysis, 32, no. 4-5 (2003):957-966,
https://doi.org/10.1016/S0731-7085(03)00197-3 . .

TY  - CONF
AU  - Kapetanović, Vera
AU  - Aleksić, Mara
AU  - Zuman, P.
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/389
AB  - All aspects of electrochemically studies of cephalosporins are presented: the reduction and oxidation processes, adsorption phenomenon, catalytic processes, complex formation, degradation, analytical determination stripping analysis, applications of different methods proposed in biological materials and drugs. In addition, the recent developments in electrochemistry of cefetamet, the cephalosporin with metoxyimino group in the side chain of C-7 are given.
AB  - U radu su prikazani svi aspekti elektrohemijskog proučavanja cefalosporinskih antibiotika: polarografska i voltametrijska ispitivanja procesa redukcije i oksidacije, fenomen adsorpcije na živinoj elektrodi, "stripping" analiza kataliti č ki talasi, formiranje kompleksa, indirektnog i direktnog određivanja u doziranim farmaceutskim oblicima i u biološkom materijalu. U drugom delu rada, prikazani su noviji rezultati dobijeni u proučavanju mehanizma redukcije cefetameta - cefalosporina sa alkoksi grupom u bočnom lancu na položaju C-7.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Electrochemistry of cephalosporins: Recent developments in mechanism study of cefetamet - metoxyimino cephalosporin
T1  - Elektrohemija cefalosporina: noviji rezultati u mehanizmu redukcije metoksiimino cefalosporina-cefetameta
VL  - 52
IS  - 3
SP  - 157
EP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_farfar_389
ER  - 

@conference{
author = "Kapetanović, Vera and Aleksić, Mara and Zuman, P.",
year = "2002",
abstract = "All aspects of electrochemically studies of cephalosporins are presented: the reduction and oxidation processes, adsorption phenomenon, catalytic processes, complex formation, degradation, analytical determination stripping analysis, applications of different methods proposed in biological materials and drugs. In addition, the recent developments in electrochemistry of cefetamet, the cephalosporin with metoxyimino group in the side chain of C-7 are given., U radu su prikazani svi aspekti elektrohemijskog proučavanja cefalosporinskih antibiotika: polarografska i voltametrijska ispitivanja procesa redukcije i oksidacije, fenomen adsorpcije na živinoj elektrodi, "stripping" analiza kataliti č ki talasi, formiranje kompleksa, indirektnog i direktnog određivanja u doziranim farmaceutskim oblicima i u biološkom materijalu. U drugom delu rada, prikazani su noviji rezultati dobijeni u proučavanju mehanizma redukcije cefetameta - cefalosporina sa alkoksi grupom u bočnom lancu na položaju C-7.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Electrochemistry of cephalosporins: Recent developments in mechanism study of cefetamet - metoxyimino cephalosporin, Elektrohemija cefalosporina: noviji rezultati u mehanizmu redukcije metoksiimino cefalosporina-cefetameta",
volume = "52",
number = "3",
pages = "157-166",
url = "https://hdl.handle.net/21.15107/rcub_farfar_389"
}

Kapetanović, V., Aleksić, M.,& Zuman, P.. (2002). Electrochemistry of cephalosporins: Recent developments in mechanism study of cefetamet - metoxyimino cephalosporin. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 52(3), 157-166.
https://hdl.handle.net/21.15107/rcub_farfar_389

Kapetanović V, Aleksić M, Zuman P. Electrochemistry of cephalosporins: Recent developments in mechanism study of cefetamet - metoxyimino cephalosporin. in Arhiv za farmaciju. 2002;52(3):157-166.
https://hdl.handle.net/21.15107/rcub_farfar_389 .

Kapetanović, Vera, Aleksić, Mara, Zuman, P., "Electrochemistry of cephalosporins: Recent developments in mechanism study of cefetamet - metoxyimino cephalosporin" in Arhiv za farmaciju, 52, no. 3 (2002):157-166,
https://hdl.handle.net/21.15107/rcub_farfar_389 .

TY  - JOUR
AU  - Kapetanović, Vera
AU  - Aleksić, Mara
AU  - Zuman, P.
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5447
AB  - Abstract
Most oximes as well as their N-alkyl and O-alkyl derivatives are electrochemically reduced in a single four-electron step to the amine. Some exceptions have been reported, where the reduction occurs in two two-electron steps. The reduction of the O-methyloxime grouping in the antibiotic cefetamet occurs at pH 5-9 in two steps, corresponding to a reduction to the imine and amine, respectively. It has been shown that the separation of the two two-electron processes is caused by differences in position and rate of establishment of acid-base equilibria resulting in protonations of the oxime and imino grouping. © 2001 Elsevier Science B.V.
PB  - Elsevier
T2  - Journal of Electroanalytical Chemistry
T1  - Two-step reduction of the O-methyloxime group in the antibiotic cefetamet
VL  - 507
SP  - 263
EP  - 267
DO  - 10.1016/S0022-0728(01)00507-1
ER  - 

@article{
author = "Kapetanović, Vera and Aleksić, Mara and Zuman, P.",
year = "2001",
abstract = "Abstract
Most oximes as well as their N-alkyl and O-alkyl derivatives are electrochemically reduced in a single four-electron step to the amine. Some exceptions have been reported, where the reduction occurs in two two-electron steps. The reduction of the O-methyloxime grouping in the antibiotic cefetamet occurs at pH 5-9 in two steps, corresponding to a reduction to the imine and amine, respectively. It has been shown that the separation of the two two-electron processes is caused by differences in position and rate of establishment of acid-base equilibria resulting in protonations of the oxime and imino grouping. © 2001 Elsevier Science B.V.",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Two-step reduction of the O-methyloxime group in the antibiotic cefetamet",
volume = "507",
pages = "263-267",
doi = "10.1016/S0022-0728(01)00507-1"
}

Kapetanović, V., Aleksić, M.,& Zuman, P.. (2001). Two-step reduction of the O-methyloxime group in the antibiotic cefetamet. in Journal of Electroanalytical Chemistry
Elsevier., 507, 263-267.
https://doi.org/10.1016/S0022-0728(01)00507-1

Kapetanović V, Aleksić M, Zuman P. Two-step reduction of the O-methyloxime group in the antibiotic cefetamet. in Journal of Electroanalytical Chemistry. 2001;507:263-267.
doi:10.1016/S0022-0728(01)00507-1 .

Kapetanović, Vera, Aleksić, Mara, Zuman, P., "Two-step reduction of the O-methyloxime group in the antibiotic cefetamet" in Journal of Electroanalytical Chemistry, 507 (2001):263-267,
https://doi.org/10.1016/S0022-0728(01)00507-1 . .