TY  - JOUR
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Antić-Stanković, Jelena
AU  - Arsenović-Ranin, Nevena
AU  - Bufan, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5582
AB  - The normal human microbiota, formerly called the "microbial flora," consists of bacteria,
fungi, viruses, and parasites that colonise the skin and mucous membranes of the respiratory,
gastrointestinal, and genitourinary tracts. The number and diversity of microorganisms varies
between different body niches and is greatest in the intestinal tract. The microbiota contributes to
the homeostasis of the human organism by preventing colonisation by pathogenic
microorganisms, participating in digestive processes and metabolism, and regulating immune
functions.
Various environmental and genetic factors can lead to an imbalance in the human
microbiota, called dysbiosis, which can affect human health. Dysbiosis is usually the result of
decreased microbial diversity and a lower number of saprophytic microorganisms, followed by
an overgrowth of opportunistic species. The most common diseases directly related to intestinal
dysbiosis are antibiotic-associated diarrhoea and pseudomembranous colitis, both of which are
associated with the excessive growth of harmful bacteria and Clostridioides difficile following
broad-spectrum antibiotic therapy.
Dysbiosis is associated with various health conditions or diseases such as acne, psoriasis,
eczema, chronic obstructive pulmonary disease, inflammatory bowel disease, obesity, metabolic
syndrome, type 2 diabetes, autoimmune diseases and allergies, neurological diseases such as
Parkinson's disease, Alzheimer's disease, epilepsy and stroke, depression, anxiety, infertility,
preterm birth, and malignancies.
AB  - Normalna ljudska mikrobiota, koja se ranije nazivala „mikroflora“, sastoji se od bakterija,
gljivica, virusa i parazita koji kolonizuju kožu i sluzokožu respiratornog, gastrointestinalnog i
genitourinarnog trakta. Broj i raznovrsnost mikroorganizama variraju između različitih telesnih
niša i najveći su u crevnom traktu. Mikrobiota doprinosi homeostazi ljudskog organizma tako što
sprečava kolonizaciju patogenim mikroorganizmima, učestvuje u procesima varenja i
metabolizma i reguliše imunološke funkcije.
Disbioza je stanje u kome dolazi do neravnoteže sastava mikrobiote usled uticaja različitih
egzogenih ili endogenih faktora, što može uticati na ljudsko zdravlje. Ona je najčešće rezultat
smanjene raznovrsnosti mikroorganizama i manjeg broja saprofitnih bakterija, što je praćeno
prekomernim rastom potencijalno štetnih vrsta. Najčešće bolesti koje su direktno povezane sa
crevnom disbiozom su dijareja povezana sa primenom antibiotika i pseudomembranozni kolitis,
a obe nastaju kao posledica prekomernog rasta štetnih bakterija i Clostridioides difficile nakon
terapije antibioticima širokog spektra.
Disbioza je povezana sa različitim zdravstvenim stanjima ili bolestima kao što su akne,
psorijaza, ekcem, hronična opstruktivna bolest pluća, inflamatorna bolest creva, gojaznost,
metabolički sindrom, dijabetes tipa 2, autoimunske bolesti i alergije, neurološke bolesti kao što
su Parkinsonova bolest, Alchajmerova demencija, epilepsija i moždani udar, depresija,
anksioznost, neplodnost, prevremeni porođaj i maligni tumori.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
PB  - Beograd : Univerzitet u Beogradu - Farmaceutski fakultet
T2  - Arhiv za farmaciju
T1  - Normal human microbiota and dysbiosis - implications for health and disease
T1  - Normalna ljudska mikrobiota i disbioza - implikacije po zdravlje i bolest
VL  - 74
IS  - 1
SP  - 1
EP  - 22
DO  - 10.5937/arhfarm74-46612
ER  - 

@article{
author = "Božić, Dragana and Milenković, Marina and Antić-Stanković, Jelena and Arsenović-Ranin, Nevena and Bufan, Biljana",
year = "2024",
abstract = "The normal human microbiota, formerly called the "microbial flora," consists of bacteria,
fungi, viruses, and parasites that colonise the skin and mucous membranes of the respiratory,
gastrointestinal, and genitourinary tracts. The number and diversity of microorganisms varies
between different body niches and is greatest in the intestinal tract. The microbiota contributes to
the homeostasis of the human organism by preventing colonisation by pathogenic
microorganisms, participating in digestive processes and metabolism, and regulating immune
functions.
Various environmental and genetic factors can lead to an imbalance in the human
microbiota, called dysbiosis, which can affect human health. Dysbiosis is usually the result of
decreased microbial diversity and a lower number of saprophytic microorganisms, followed by
an overgrowth of opportunistic species. The most common diseases directly related to intestinal
dysbiosis are antibiotic-associated diarrhoea and pseudomembranous colitis, both of which are
associated with the excessive growth of harmful bacteria and Clostridioides difficile following
broad-spectrum antibiotic therapy.
Dysbiosis is associated with various health conditions or diseases such as acne, psoriasis,
eczema, chronic obstructive pulmonary disease, inflammatory bowel disease, obesity, metabolic
syndrome, type 2 diabetes, autoimmune diseases and allergies, neurological diseases such as
Parkinson's disease, Alzheimer's disease, epilepsy and stroke, depression, anxiety, infertility,
preterm birth, and malignancies., Normalna ljudska mikrobiota, koja se ranije nazivala „mikroflora“, sastoji se od bakterija,
gljivica, virusa i parazita koji kolonizuju kožu i sluzokožu respiratornog, gastrointestinalnog i
genitourinarnog trakta. Broj i raznovrsnost mikroorganizama variraju između različitih telesnih
niša i najveći su u crevnom traktu. Mikrobiota doprinosi homeostazi ljudskog organizma tako što
sprečava kolonizaciju patogenim mikroorganizmima, učestvuje u procesima varenja i
metabolizma i reguliše imunološke funkcije.
Disbioza je stanje u kome dolazi do neravnoteže sastava mikrobiote usled uticaja različitih
egzogenih ili endogenih faktora, što može uticati na ljudsko zdravlje. Ona je najčešće rezultat
smanjene raznovrsnosti mikroorganizama i manjeg broja saprofitnih bakterija, što je praćeno
prekomernim rastom potencijalno štetnih vrsta. Najčešće bolesti koje su direktno povezane sa
crevnom disbiozom su dijareja povezana sa primenom antibiotika i pseudomembranozni kolitis,
a obe nastaju kao posledica prekomernog rasta štetnih bakterija i Clostridioides difficile nakon
terapije antibioticima širokog spektra.
Disbioza je povezana sa različitim zdravstvenim stanjima ili bolestima kao što su akne,
psorijaza, ekcem, hronična opstruktivna bolest pluća, inflamatorna bolest creva, gojaznost,
metabolički sindrom, dijabetes tipa 2, autoimunske bolesti i alergije, neurološke bolesti kao što
su Parkinsonova bolest, Alchajmerova demencija, epilepsija i moždani udar, depresija,
anksioznost, neplodnost, prevremeni porođaj i maligni tumori.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije, Beograd : Univerzitet u Beogradu - Farmaceutski fakultet",
journal = "Arhiv za farmaciju",
title = "Normal human microbiota and dysbiosis - implications for health and disease, Normalna ljudska mikrobiota i disbioza - implikacije po zdravlje i bolest",
volume = "74",
number = "1",
pages = "1-22",
doi = "10.5937/arhfarm74-46612"
}

Božić, D., Milenković, M., Antić-Stanković, J., Arsenović-Ranin, N.,& Bufan, B.. (2024). Normal human microbiota and dysbiosis - implications for health and disease. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 74(1), 1-22.
https://doi.org/10.5937/arhfarm74-46612

Božić D, Milenković M, Antić-Stanković J, Arsenović-Ranin N, Bufan B. Normal human microbiota and dysbiosis - implications for health and disease. in Arhiv za farmaciju. 2024;74(1):1-22.
doi:10.5937/arhfarm74-46612 .

Božić, Dragana, Milenković, Marina, Antić-Stanković, Jelena, Arsenović-Ranin, Nevena, Bufan, Biljana, "Normal human microbiota and dysbiosis - implications for health and disease" in Arhiv za farmaciju, 74, no. 1 (2024):1-22,
https://doi.org/10.5937/arhfarm74-46612 . .

TY  - JOUR
AU  - Vukašinović, Mila
AU  - Pantelić, Ivana
AU  - Savić, Sanela
AU  - Cekić, Nebojša
AU  - Vukašinović Sekulić, Maja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Tošić, Anđela
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5311
AB  - Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.
PB  - MDPI
T2  - Cosmetics
T1  - Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety
VL  - 10
IS  - 6
DO  - 10.3390/cosmetics10060162
ER  - 

@article{
author = "Vukašinović, Mila and Pantelić, Ivana and Savić, Sanela and Cekić, Nebojša and Vukašinović Sekulić, Maja and Antić-Stanković, Jelena and Božić, Dragana and Tošić, Anđela and Tamburić, Slobodanka and Savić, Snežana",
year = "2023",
abstract = "Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.",
publisher = "MDPI",
journal = "Cosmetics",
title = "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety",
volume = "10",
number = "6",
doi = "10.3390/cosmetics10060162"
}

Vukašinović, M., Pantelić, I., Savić, S., Cekić, N., Vukašinović Sekulić, M., Antić-Stanković, J., Božić, D., Tošić, A., Tamburić, S.,& Savić, S.. (2023). Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics
MDPI., 10(6).
https://doi.org/10.3390/cosmetics10060162

Vukašinović M, Pantelić I, Savić S, Cekić N, Vukašinović Sekulić M, Antić-Stanković J, Božić D, Tošić A, Tamburić S, Savić S. Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics. 2023;10(6).
doi:10.3390/cosmetics10060162 .

Vukašinović, Mila, Pantelić, Ivana, Savić, Sanela, Cekić, Nebojša, Vukašinović Sekulić, Maja, Antić-Stanković, Jelena, Božić, Dragana, Tošić, Anđela, Tamburić, Slobodanka, Savić, Snežana, "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety" in Cosmetics, 10, no. 6 (2023),
https://doi.org/10.3390/cosmetics10060162 . .

TY  - CONF
AU  - Dabetić, Nevena
AU  - Todorović, Vanja
AU  - Božić, Dragana
AU  - Antić-Stanković, Jelena
AU  - Šobajić, Slađana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5140
AB  - Semenke predstavljaju najvredniji deo ploda vinove loze. Polifenolna jedinjenja se primarno
koncentrišu tamo, zbog čega semenke poseduju jaku antioksidativnu aktivnost. Zahvaljujući tome,
ekstrakti semenki mogu delovati kao antimikrobni agensi. Ekstrakcija bioaktivnih jedinjenja se
obično vrši pomoću isparljivih, organskih rastvarača. Poslednjih godina, zajedno sa povećanjem
svesti o značaju zaštite životne sredine, različiti „zeleni“ rastvarači su se pojavili kao alternativa
konvencionalnim.
Ova studija je imala za cilj ispitivanje razlika između semenki različitih sorti grožđa u pogledu
njihove antimikrobne aktivnosti prema Staphylococcus aureus. Takođe je procenjen afinitet
eutektičkog rastvarača za ekstrakciju polifenola iz semenki grožđa. Kao referentni rastvarač
korišćen je etanol.
Semenke su izolovane iz pet različitih sorti crvenog grožđa koje se široko gaje u Srbiji.
Ultrazvučna ekstrakcija izvedena je korišćenjem eutektičkog rastvarača (holin hlorid:limunska
kiselina, 2:1, 30 % vode; ChCit) i 70 % etanola. Ukupan sadržaj polifenola (eng. Total Phenolic
Content; TPC) je određen Folin-Ciocalteu metodom. Antimikrobna aktivnost uzoraka je ispitivana
je na standardnom soju Staphylococcus aureus ATCC 6538 bujon-mikrodilucionim testom.
TPC vrednosti su se kretale od 91,75 do 116,71 i od 84,79 do 135,5 mg GAE/g suve mase (za
ChCit i etanolne ekstrakte, respektivno). Semenke sorte Prokupac imale su najveći sadržaj ukupnih
polifenola, bez obzira na upotrebljeni rastvarač. Vrednosti minimalnih inhibitornih koncentracija
(MIK) su se kretale između 0,04 i 0,15 mg/ml. ChCit ekstrakti su bili slični ili čak efikasniji protiv
ove Gram-pozitivne bakterije u poređenju sa etanolnim ekstraktima. Ovo bi moglo biti delimično
objašnjeno antimikrobnom aktivnošću samog rastvarača, pošto je primećeno da ChCit inhibira rast
Staphylococcus aureus (MIK=0,3 mg/ml).
Imajući u vidu da su ekstrakti pokazali znatno jaču aktivnost u odnosu na rastvarač i da su
rezultati ispitivanja antimikrobne aktivnosti bili u značajnoj korelaciji sa sadržajem polifenola,
može se zaključiti da je sinergizam između prisutnih polifenola i ChCit rezultirao snažnom
antimikrobnom aktivnošću protiv Staphylococcus aureus u ekološki prihvatljivijim ekstraktima.
PB  - Srpsko udruženje za kliničku mikrobiologiju (SUKM)
C3  - SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata
T1  - Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa
SP  - 56
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5140
ER  - 

@conference{
author = "Dabetić, Nevena and Todorović, Vanja and Božić, Dragana and Antić-Stanković, Jelena and Šobajić, Slađana",
year = "2023",
abstract = "Semenke predstavljaju najvredniji deo ploda vinove loze. Polifenolna jedinjenja se primarno
koncentrišu tamo, zbog čega semenke poseduju jaku antioksidativnu aktivnost. Zahvaljujući tome,
ekstrakti semenki mogu delovati kao antimikrobni agensi. Ekstrakcija bioaktivnih jedinjenja se
obično vrši pomoću isparljivih, organskih rastvarača. Poslednjih godina, zajedno sa povećanjem
svesti o značaju zaštite životne sredine, različiti „zeleni“ rastvarači su se pojavili kao alternativa
konvencionalnim.
Ova studija je imala za cilj ispitivanje razlika između semenki različitih sorti grožđa u pogledu
njihove antimikrobne aktivnosti prema Staphylococcus aureus. Takođe je procenjen afinitet
eutektičkog rastvarača za ekstrakciju polifenola iz semenki grožđa. Kao referentni rastvarač
korišćen je etanol.
Semenke su izolovane iz pet različitih sorti crvenog grožđa koje se široko gaje u Srbiji.
Ultrazvučna ekstrakcija izvedena je korišćenjem eutektičkog rastvarača (holin hlorid:limunska
kiselina, 2:1, 30 % vode; ChCit) i 70 % etanola. Ukupan sadržaj polifenola (eng. Total Phenolic
Content; TPC) je određen Folin-Ciocalteu metodom. Antimikrobna aktivnost uzoraka je ispitivana
je na standardnom soju Staphylococcus aureus ATCC 6538 bujon-mikrodilucionim testom.
TPC vrednosti su se kretale od 91,75 do 116,71 i od 84,79 do 135,5 mg GAE/g suve mase (za
ChCit i etanolne ekstrakte, respektivno). Semenke sorte Prokupac imale su najveći sadržaj ukupnih
polifenola, bez obzira na upotrebljeni rastvarač. Vrednosti minimalnih inhibitornih koncentracija
(MIK) su se kretale između 0,04 i 0,15 mg/ml. ChCit ekstrakti su bili slični ili čak efikasniji protiv
ove Gram-pozitivne bakterije u poređenju sa etanolnim ekstraktima. Ovo bi moglo biti delimično
objašnjeno antimikrobnom aktivnošću samog rastvarača, pošto je primećeno da ChCit inhibira rast
Staphylococcus aureus (MIK=0,3 mg/ml).
Imajući u vidu da su ekstrakti pokazali znatno jaču aktivnost u odnosu na rastvarač i da su
rezultati ispitivanja antimikrobne aktivnosti bili u značajnoj korelaciji sa sadržajem polifenola,
može se zaključiti da je sinergizam između prisutnih polifenola i ChCit rezultirao snažnom
antimikrobnom aktivnošću protiv Staphylococcus aureus u ekološki prihvatljivijim ekstraktima.",
publisher = "Srpsko udruženje za kliničku mikrobiologiju (SUKM)",
journal = "SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata",
title = "Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa",
pages = "56-56",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5140"
}

Dabetić, N., Todorović, V., Božić, D., Antić-Stanković, J.,& Šobajić, S.. (2023). Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa. in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata
Srpsko udruženje za kliničku mikrobiologiju (SUKM)., 56-56.
https://hdl.handle.net/21.15107/rcub_farfar_5140

Dabetić N, Todorović V, Božić D, Antić-Stanković J, Šobajić S. Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa. in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata. 2023;:56-56.
https://hdl.handle.net/21.15107/rcub_farfar_5140 .

Dabetić, Nevena, Todorović, Vanja, Božić, Dragana, Antić-Stanković, Jelena, Šobajić, Slađana, "Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa" in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata (2023):56-56,
https://hdl.handle.net/21.15107/rcub_farfar_5140 .

TY  - JOUR
AU  - Tomić, Tanja
AU  - Henman, Martin
AU  - Tadić, Ivana
AU  - Antić-Stanković, Jelena
AU  - Santric Milicević, Milena
AU  - Maksimović, Nataša
AU  - Odalović, Marina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4832
AB  - Background In Europe, Serbia occupies a high position in antibiotic utilization and antimicrobial resistance (AMR). Aim The aim was to analyse utilization trends of meropenem, ceftazidime, aminoglycosides, piperacillin/tazobactam and fluoroquinolones (2006–2020), and the reported AMR in Pseudomonas aeruginosa (2013–2020) in Serbia and to compare with data from eight European countries (2015–2020). Method Joinpoint regression was used to analyse antibiotic utilization data (2006–2020) and the reported AMR in Pseu- domonas aeruginosa (2013–2020). Data sources were relevant national and international institutions. Antibiotic utilization and AMR in Pseudomonas aeruginosa data in Serbia were compared with eight European countries. Results There was a significantly increased trend for ceftazidime utilization and reported resistance in Pseudomonas aer- uginosa, Serbia (p < 0.05) (2018–2020). For ceftazidime, piperacillin/tazobactam, and fluoroquinolones resistances in Pseu- domonas aeruginosa an increased trend was observed, Serbia (2013–2020). A decrease in both the utilization of aminogly- cosides, Serbia (p < 0.05) (2006–2018) and contemporaneous Pseudomonas aeruginosa resistance (p > 0.05) was detected. Fluoroquinolone utilization (2015–2020) was highest in Serbia compared to Netherlands and Finland, 310 and 305% higher, similar compared to Romania, and 2% less compared to Montenegro. Aminoglycosides (2015–2020) were 2550 and 783% more used in Serbia compared to Finland and Netherlands, and 38% less regarding Montenegro. The highest percentage of Pseudomonas aeruginosa resistance was in Romania and Serbia (2015–2020). Conclusion The use of piperacillin/tazobactam, ceftazidime and fluoroquinolones should be carefully monitored in clinical practice due to increased Pseudomonas aeruginosa resistance. The level of utilization and AMR in Pseudomonas aeruginosa is still high in Serbia compared to other European countries.
PB  - Springer
T2  - International Journal of Clinical Pharmacy
T1  - Antimicrobial utilization and resistance in Pseudomonas aeruginosa using segmented regression analysis: a comparative study between Serbia and eight European Countries
DO  - 10.1007/s11096-023-01603-y
ER  - 

@article{
author = "Tomić, Tanja and Henman, Martin and Tadić, Ivana and Antić-Stanković, Jelena and Santric Milicević, Milena and Maksimović, Nataša and Odalović, Marina",
year = "2023",
abstract = "Background In Europe, Serbia occupies a high position in antibiotic utilization and antimicrobial resistance (AMR). Aim The aim was to analyse utilization trends of meropenem, ceftazidime, aminoglycosides, piperacillin/tazobactam and fluoroquinolones (2006–2020), and the reported AMR in Pseudomonas aeruginosa (2013–2020) in Serbia and to compare with data from eight European countries (2015–2020). Method Joinpoint regression was used to analyse antibiotic utilization data (2006–2020) and the reported AMR in Pseu- domonas aeruginosa (2013–2020). Data sources were relevant national and international institutions. Antibiotic utilization and AMR in Pseudomonas aeruginosa data in Serbia were compared with eight European countries. Results There was a significantly increased trend for ceftazidime utilization and reported resistance in Pseudomonas aer- uginosa, Serbia (p < 0.05) (2018–2020). For ceftazidime, piperacillin/tazobactam, and fluoroquinolones resistances in Pseu- domonas aeruginosa an increased trend was observed, Serbia (2013–2020). A decrease in both the utilization of aminogly- cosides, Serbia (p < 0.05) (2006–2018) and contemporaneous Pseudomonas aeruginosa resistance (p > 0.05) was detected. Fluoroquinolone utilization (2015–2020) was highest in Serbia compared to Netherlands and Finland, 310 and 305% higher, similar compared to Romania, and 2% less compared to Montenegro. Aminoglycosides (2015–2020) were 2550 and 783% more used in Serbia compared to Finland and Netherlands, and 38% less regarding Montenegro. The highest percentage of Pseudomonas aeruginosa resistance was in Romania and Serbia (2015–2020). Conclusion The use of piperacillin/tazobactam, ceftazidime and fluoroquinolones should be carefully monitored in clinical practice due to increased Pseudomonas aeruginosa resistance. The level of utilization and AMR in Pseudomonas aeruginosa is still high in Serbia compared to other European countries.",
publisher = "Springer",
journal = "International Journal of Clinical Pharmacy",
title = "Antimicrobial utilization and resistance in Pseudomonas aeruginosa using segmented regression analysis: a comparative study between Serbia and eight European Countries",
doi = "10.1007/s11096-023-01603-y"
}

Tomić, T., Henman, M., Tadić, I., Antić-Stanković, J., Santric Milicević, M., Maksimović, N.,& Odalović, M.. (2023). Antimicrobial utilization and resistance in Pseudomonas aeruginosa using segmented regression analysis: a comparative study between Serbia and eight European Countries. in International Journal of Clinical Pharmacy
Springer..
https://doi.org/10.1007/s11096-023-01603-y

Tomić T, Henman M, Tadić I, Antić-Stanković J, Santric Milicević M, Maksimović N, Odalović M. Antimicrobial utilization and resistance in Pseudomonas aeruginosa using segmented regression analysis: a comparative study between Serbia and eight European Countries. in International Journal of Clinical Pharmacy. 2023;.
doi:10.1007/s11096-023-01603-y .

Tomić, Tanja, Henman, Martin, Tadić, Ivana, Antić-Stanković, Jelena, Santric Milicević, Milena, Maksimović, Nataša, Odalović, Marina, "Antimicrobial utilization and resistance in Pseudomonas aeruginosa using segmented regression analysis: a comparative study between Serbia and eight European Countries" in International Journal of Clinical Pharmacy (2023),
https://doi.org/10.1007/s11096-023-01603-y . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4919
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 

@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}

Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J. M.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis
Oxford University Press., 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013

Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković JM. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .

Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan M., "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .

TY  - JOUR
AU  - Krstić, Sanja
AU  - Miljić, Milorad
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5034
AB  - Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.
PB  - Elsevier Ltd
T2  - Food Chemistry Advances
T1  - Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity
VL  - 3
DO  - 10.1016/j.focha.2023.100395
ER  - 

@article{
author = "Krstić, Sanja and Miljić, Milorad and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Pirković, Andrea",
year = "2023",
abstract = "Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry Advances",
title = "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity",
volume = "3",
doi = "10.1016/j.focha.2023.100395"
}

Krstić, S., Miljić, M., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Pirković, A.. (2023). Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances
Elsevier Ltd., 3.
https://doi.org/10.1016/j.focha.2023.100395

Krstić S, Miljić M, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Pirković A. Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances. 2023;3.
doi:10.1016/j.focha.2023.100395 .

Krstić, Sanja, Miljić, Milorad, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Pirković, Andrea, "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity" in Food Chemistry Advances, 3 (2023),
https://doi.org/10.1016/j.focha.2023.100395 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Simić, Mitar
AU  - Pantelić, Ivana
AU  - Stojanović, Goran
AU  - Antić-Stanković, Jelena
AU  - Marković, Bojan
AU  - Savić, Snežana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4328
AB  - So far, various approaches have been proposed to improve dermal drug delivery. The
use of chemical penetration enhancers has a long history of application, while methods based on
the electrical current (such as iontophoresis) stand out as promising “active” techniques. Aiming to
evaluate the contribution of different approaches to dermal delivery, in this work curcumin-loaded
nanoemulsions with and without monoterpenes (eucalyptol or pinene) as chemical penetration
enhancers, and a custom-made adhesive dermal delivery system based on iontophoresis were
designed and assessed. In an in vivo study applying skin bioengineering techniques, their safety
profile was proven. Three examined iontophoresis protocols, with total skin exposure time of 15 min
(continuous flow for 15 min (15-0); 3 min of continuous flow and 2 min pause (3-2; 5 cycles) and
5 min of continuous flow and 1 min pause (5-1; 3 cycles) were equally efficient in terms of the
total amount of curcumin that penetrated through the superficial skin layers ( in vivo tape stripping)
(Q3-2 = 7.04 ± 3.21 μg/cm2; Q5-1 = 6.66 ± 2.11 μg/cm2; Q15-0 = 6.96 ± 3.21 μg/cm2), significantly
more efficient compared to the referent nanoemulsion and monoterpene-containing nanoemulsions.
Further improvement of an efficient mobile adhesive system for iontophoresis would be a practical
contribution in the field of dermal drug application.
PB  - MDPI
T2  - Pharmaceutics
T1  - Chemical vs. Physical Methods to Improve Dermal Drug Delivery: A Case Study with Nanoemulsions and Iontophoresis
VL  - 14
IS  - 6
DO  - 10.3390/pharmaceutics14061144
ER  - 

@article{
author = "Nikolić, Ines and Simić, Mitar and Pantelić, Ivana and Stojanović, Goran and Antić-Stanković, Jelena and Marković, Bojan and Savić, Snežana",
year = "2022",
abstract = "So far, various approaches have been proposed to improve dermal drug delivery. The
use of chemical penetration enhancers has a long history of application, while methods based on
the electrical current (such as iontophoresis) stand out as promising “active” techniques. Aiming to
evaluate the contribution of different approaches to dermal delivery, in this work curcumin-loaded
nanoemulsions with and without monoterpenes (eucalyptol or pinene) as chemical penetration
enhancers, and a custom-made adhesive dermal delivery system based on iontophoresis were
designed and assessed. In an in vivo study applying skin bioengineering techniques, their safety
profile was proven. Three examined iontophoresis protocols, with total skin exposure time of 15 min
(continuous flow for 15 min (15-0); 3 min of continuous flow and 2 min pause (3-2; 5 cycles) and
5 min of continuous flow and 1 min pause (5-1; 3 cycles) were equally efficient in terms of the
total amount of curcumin that penetrated through the superficial skin layers ( in vivo tape stripping)
(Q3-2 = 7.04 ± 3.21 μg/cm2; Q5-1 = 6.66 ± 2.11 μg/cm2; Q15-0 = 6.96 ± 3.21 μg/cm2), significantly
more efficient compared to the referent nanoemulsion and monoterpene-containing nanoemulsions.
Further improvement of an efficient mobile adhesive system for iontophoresis would be a practical
contribution in the field of dermal drug application.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Chemical vs. Physical Methods to Improve Dermal Drug Delivery: A Case Study with Nanoemulsions and Iontophoresis",
volume = "14",
number = "6",
doi = "10.3390/pharmaceutics14061144"
}

Nikolić, I., Simić, M., Pantelić, I., Stojanović, G., Antić-Stanković, J., Marković, B.,& Savić, S.. (2022). Chemical vs. Physical Methods to Improve Dermal Drug Delivery: A Case Study with Nanoemulsions and Iontophoresis. in Pharmaceutics
MDPI., 14(6).
https://doi.org/10.3390/pharmaceutics14061144

Nikolić I, Simić M, Pantelić I, Stojanović G, Antić-Stanković J, Marković B, Savić S. Chemical vs. Physical Methods to Improve Dermal Drug Delivery: A Case Study with Nanoemulsions and Iontophoresis. in Pharmaceutics. 2022;14(6).
doi:10.3390/pharmaceutics14061144 .

Nikolić, Ines, Simić, Mitar, Pantelić, Ivana, Stojanović, Goran, Antić-Stanković, Jelena, Marković, Bojan, Savić, Snežana, "Chemical vs. Physical Methods to Improve Dermal Drug Delivery: A Case Study with Nanoemulsions and Iontophoresis" in Pharmaceutics, 14, no. 6 (2022),
https://doi.org/10.3390/pharmaceutics14061144 . .

TY  - JOUR
AU  - Janošević-Ležaić, Aleksandra
AU  - Pašti, Igor
AU  - Gledović, Ana
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Uskoković-Marković, Snežana
AU  - Ćirić-Marjanović, Gordana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4067
AB  - Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.
PB  - Elsevier Ltd
T2  - Synthetic Metals
T1  - Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities
VL  - 286
DO  - 10.1016/j.synthmet.2022.117048
ER  - 

@article{
author = "Janošević-Ležaić, Aleksandra and Pašti, Igor and Gledović, Ana and Antić-Stanković, Jelena and Božić, Dragana and Uskoković-Marković, Snežana and Ćirić-Marjanović, Gordana",
year = "2022",
abstract = "Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.",
publisher = "Elsevier Ltd",
journal = "Synthetic Metals",
title = "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities",
volume = "286",
doi = "10.1016/j.synthmet.2022.117048"
}

Janošević-Ležaić, A., Pašti, I., Gledović, A., Antić-Stanković, J., Božić, D., Uskoković-Marković, S.,& Ćirić-Marjanović, G.. (2022). Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals
Elsevier Ltd., 286.
https://doi.org/10.1016/j.synthmet.2022.117048

Janošević-Ležaić A, Pašti I, Gledović A, Antić-Stanković J, Božić D, Uskoković-Marković S, Ćirić-Marjanović G. Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals. 2022;286.
doi:10.1016/j.synthmet.2022.117048 .

Janošević-Ležaić, Aleksandra, Pašti, Igor, Gledović, Ana, Antić-Stanković, Jelena, Božić, Dragana, Uskoković-Marković, Snežana, Ćirić-Marjanović, Gordana, "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities" in Synthetic Metals, 286 (2022),
https://doi.org/10.1016/j.synthmet.2022.117048 . .

TY  - JOUR
AU  - Mijatović, Stefan
AU  - Antić-Stanković, Jelena
AU  - Čolović Čalovski, Ivana, Ivana
AU  - Dubljanin, Eleonora
AU  - Pljevljakušić, Dejan
AU  - Bigović, Dubravka
AU  - Džamić, Aleksandar
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4292
AB  - The aim of our study was to determine the susceptibility of 15 Candida albicans sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of Lavandula angustifolia essential oil (LAEO). The commercial LAEO was analyzed using gas chromatography-mass spectrometry. The antifungal activity was evaluated using EUCAST protocol. A killing assay was performed to evaluate kinetics of 2% LAEO within 30 min treatment. LAEO with major constituents’ linalool (33.4%) and linalyl acetate (30.5%) effective inhibited grows of C. albicans in concentration range 0.5–2%. Fluconazole activity was noted in 67% of the isolates with MICs in range 0.06–1 µg/mL. Surprisingly, 40% of isolates were non-wild-type (non-WT), while MICs for WT ranged between 0.125–0.25 µg/mL. There were no significant differences in the LAEO MICs among fluconazole-resistant and fluconazole-susceptible sputum strains (p = 0.31) and neither among caspofungin non-WT and WT isolates (p = 0.79). The 2% LAEO rapidly achieved 50% growth reduction in all tested strains between 0.2 and 3.5 min. Within 30 min, the same LAEO concentration exhibited a 99.9% reduction in 27% isolates. This study demonstrated that 2% solution of LAEO showed a significant antifungal activity which is equally effective against fluconazole and caspofungin susceptible and less-susceptible strains.
PB  - MDPI
T2  - Molecules
T1  - Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates
VL  - 27
IS  - 19
DO  - 10.3390/molecules27196300
ER  - 

@article{
author = "Mijatović, Stefan and Antić-Stanković, Jelena and Čolović Čalovski, Ivana, Ivana and Dubljanin, Eleonora and Pljevljakušić, Dejan and Bigović, Dubravka and Džamić, Aleksandar",
year = "2022",
abstract = "The aim of our study was to determine the susceptibility of 15 Candida albicans sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of Lavandula angustifolia essential oil (LAEO). The commercial LAEO was analyzed using gas chromatography-mass spectrometry. The antifungal activity was evaluated using EUCAST protocol. A killing assay was performed to evaluate kinetics of 2% LAEO within 30 min treatment. LAEO with major constituents’ linalool (33.4%) and linalyl acetate (30.5%) effective inhibited grows of C. albicans in concentration range 0.5–2%. Fluconazole activity was noted in 67% of the isolates with MICs in range 0.06–1 µg/mL. Surprisingly, 40% of isolates were non-wild-type (non-WT), while MICs for WT ranged between 0.125–0.25 µg/mL. There were no significant differences in the LAEO MICs among fluconazole-resistant and fluconazole-susceptible sputum strains (p = 0.31) and neither among caspofungin non-WT and WT isolates (p = 0.79). The 2% LAEO rapidly achieved 50% growth reduction in all tested strains between 0.2 and 3.5 min. Within 30 min, the same LAEO concentration exhibited a 99.9% reduction in 27% isolates. This study demonstrated that 2% solution of LAEO showed a significant antifungal activity which is equally effective against fluconazole and caspofungin susceptible and less-susceptible strains.",
publisher = "MDPI",
journal = "Molecules",
title = "Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates",
volume = "27",
number = "19",
doi = "10.3390/molecules27196300"
}

Mijatović, S., Antić-Stanković, J., Čolović Čalovski, I. I., Dubljanin, E., Pljevljakušić, D., Bigović, D.,& Džamić, A.. (2022). Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates. in Molecules
MDPI., 27(19).
https://doi.org/10.3390/molecules27196300

Mijatović S, Antić-Stanković J, Čolović Čalovski II, Dubljanin E, Pljevljakušić D, Bigović D, Džamić A. Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates. in Molecules. 2022;27(19).
doi:10.3390/molecules27196300 .

Mijatović, Stefan, Antić-Stanković, Jelena, Čolović Čalovski, Ivana, Ivana, Dubljanin, Eleonora, Pljevljakušić, Dejan, Bigović, Dubravka, Džamić, Aleksandar, "Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196300 . .

TY  - CONF
AU  - Antić-Stanković, Jelena
AU  - Catić Đorđević, Aleksandra
AU  - Stojanović, Dušica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5137
AB  - The beneficial effects of probiotics have been demonstrated in wide range of pathological conditions. One of 
the major mechanisms of action is through the regulation of host immune response. Likewise, the probiotics 
that colonize the human host are most numerous in the intestines. The commensal intestinal microbiome 
contributes to increased resistance against infections, host immune system differentiation, and synthesis of 
nutrients. 
Probiotics such as Lactobacillus and Bifidobacterium can restore host-health by eliminating pathogens and 
regulating immune responses in intestinal epithelial cells. Also, circumstantial evidence has supported the 
presumption that probiotic supplementation decreases the severity of COVID-19 responses. Literature data 
show that the immunomodulating activity of vitamin D, which absorption could improve by probiotics, plays 
a role in viral infections. An analytical retrospective approach to the data of 34 pharmacies of Association 
Apoteka plus from all country region showed a significant increase in sales of probiotics (2019/20 by 29% 
and 2020/21 by 47%) and vitamin D (2020/21 registered drug 53.6 %, supplements 19.9%). In addition, sales 
of Over the counter (OTC) cold and flu medicines remained flat. In conclusion, self-care and prevention have 
been at a high position during the last three years, but still, due to serious health problems such as COVID 19, 
patients are seeking professional health services and prescription drugs.
PB  - Public Health Institute Niš
PB  - Faculty of Medicine Niš, University of Niš
PB  - Serbian Medical Society
C3  - 54th Days of Preventive Medicine International Congress - Book of Abstracts
T1  - Dietary supplementation of probiotic and vitamin d during pandemic of COVID-19
SP  - 32
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5137
ER  - 

@conference{
author = "Antić-Stanković, Jelena and Catić Đorđević, Aleksandra and Stojanović, Dušica",
year = "2022",
abstract = "The beneficial effects of probiotics have been demonstrated in wide range of pathological conditions. One of 
the major mechanisms of action is through the regulation of host immune response. Likewise, the probiotics 
that colonize the human host are most numerous in the intestines. The commensal intestinal microbiome 
contributes to increased resistance against infections, host immune system differentiation, and synthesis of 
nutrients. 
Probiotics such as Lactobacillus and Bifidobacterium can restore host-health by eliminating pathogens and 
regulating immune responses in intestinal epithelial cells. Also, circumstantial evidence has supported the 
presumption that probiotic supplementation decreases the severity of COVID-19 responses. Literature data 
show that the immunomodulating activity of vitamin D, which absorption could improve by probiotics, plays 
a role in viral infections. An analytical retrospective approach to the data of 34 pharmacies of Association 
Apoteka plus from all country region showed a significant increase in sales of probiotics (2019/20 by 29% 
and 2020/21 by 47%) and vitamin D (2020/21 registered drug 53.6 %, supplements 19.9%). In addition, sales 
of Over the counter (OTC) cold and flu medicines remained flat. In conclusion, self-care and prevention have 
been at a high position during the last three years, but still, due to serious health problems such as COVID 19, 
patients are seeking professional health services and prescription drugs.",
publisher = "Public Health Institute Niš, Faculty of Medicine Niš, University of Niš, Serbian Medical Society",
journal = "54th Days of Preventive Medicine International Congress - Book of Abstracts",
title = "Dietary supplementation of probiotic and vitamin d during pandemic of COVID-19",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5137"
}

Antić-Stanković, J., Catić Đorđević, A.,& Stojanović, D.. (2022). Dietary supplementation of probiotic and vitamin d during pandemic of COVID-19. in 54th Days of Preventive Medicine International Congress - Book of Abstracts
Public Health Institute Niš., 32-32.
https://hdl.handle.net/21.15107/rcub_farfar_5137

Antić-Stanković J, Catić Đorđević A, Stojanović D. Dietary supplementation of probiotic and vitamin d during pandemic of COVID-19. in 54th Days of Preventive Medicine International Congress - Book of Abstracts. 2022;:32-32.
https://hdl.handle.net/21.15107/rcub_farfar_5137 .

Antić-Stanković, Jelena, Catić Đorđević, Aleksandra, Stojanović, Dušica, "Dietary supplementation of probiotic and vitamin d during pandemic of COVID-19" in 54th Days of Preventive Medicine International Congress - Book of Abstracts (2022):32-32,
https://hdl.handle.net/21.15107/rcub_farfar_5137 .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Savić, Sanela
AU  - Mitrović, Jelena
AU  - Nikolić, Ines
AU  - Marković, Bojan
AU  - Ranđelović, Danijela
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Cekić, Nebojša
AU  - Stevanović, Vladimir
AU  - Batinić, Bojan
AU  - Aranđelović, Jovana
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5541
AB  - The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats
VL  - 22
IS  - 15
DO  - 10.3390/ijms22157991
ER  - 

@article{
author = "Đoković, Jelena and Savić, Sanela and Mitrović, Jelena and Nikolić, Ines and Marković, Bojan and Ranđelović, Danijela and Antić-Stanković, Jelena and Božić, Dragana and Cekić, Nebojša and Stevanović, Vladimir and Batinić, Bojan and Aranđelović, Jovana and Savić, Miroslav and Savić, Snežana",
year = "2021",
abstract = "The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats",
volume = "22",
number = "15",
doi = "10.3390/ijms22157991"
}

Đoković, J., Savić, S., Mitrović, J., Nikolić, I., Marković, B., Ranđelović, D., Antić-Stanković, J., Božić, D., Cekić, N., Stevanović, V., Batinić, B., Aranđelović, J., Savić, M.,& Savić, S.. (2021). Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences
MDPI., 22(15).
https://doi.org/10.3390/ijms22157991

Đoković J, Savić S, Mitrović J, Nikolić I, Marković B, Ranđelović D, Antić-Stanković J, Božić D, Cekić N, Stevanović V, Batinić B, Aranđelović J, Savić M, Savić S. Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences. 2021;22(15).
doi:10.3390/ijms22157991 .

Đoković, Jelena, Savić, Sanela, Mitrović, Jelena, Nikolić, Ines, Marković, Bojan, Ranđelović, Danijela, Antić-Stanković, Jelena, Božić, Dragana, Cekić, Nebojša, Stevanović, Vladimir, Batinić, Bojan, Aranđelović, Jovana, Savić, Miroslav, Savić, Snežana, "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats" in International Journal of Molecular Sciences, 22, no. 15 (2021),
https://doi.org/10.3390/ijms22157991 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Nikolić, Ines
AU  - Tasić-Kostov, Marija
AU  - Antić-Stanković, Jelena
AU  - Krstonošić, Veljko
AU  - Ranđelović, Danijela
AU  - Božić, Dragana
AU  - Ilić, Dušan
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3777
AB  - This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.
PB  - MDPI AG
T2  - Nanomaterials
T1  - Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance
VL  - 11
IS  - 1
SP  - 1
EP  - 21
DO  - 10.3390/nano11010217
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Nikolić, Ines and Tasić-Kostov, Marija and Antić-Stanković, Jelena and Krstonošić, Veljko and Ranđelović, Danijela and Božić, Dragana and Ilić, Dušan and Tamburić, Slobodanka and Savić, Snežana",
year = "2021",
abstract = "This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.",
publisher = "MDPI AG",
journal = "Nanomaterials",
title = "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance",
volume = "11",
number = "1",
pages = "1-21",
doi = "10.3390/nano11010217"
}

Gledović, A., Janošević-Ležaić, A., Nikolić, I., Tasić-Kostov, M., Antić-Stanković, J., Krstonošić, V., Ranđelović, D., Božić, D., Ilić, D., Tamburić, S.,& Savić, S.. (2021). Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials
MDPI AG., 11(1), 1-21.
https://doi.org/10.3390/nano11010217

Gledović A, Janošević-Ležaić A, Nikolić I, Tasić-Kostov M, Antić-Stanković J, Krstonošić V, Ranđelović D, Božić D, Ilić D, Tamburić S, Savić S. Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials. 2021;11(1):1-21.
doi:10.3390/nano11010217 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Nikolić, Ines, Tasić-Kostov, Marija, Antić-Stanković, Jelena, Krstonošić, Veljko, Ranđelović, Danijela, Božić, Dragana, Ilić, Dušan, Tamburić, Slobodanka, Savić, Snežana, "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance" in Nanomaterials, 11, no. 1 (2021):1-21,
https://doi.org/10.3390/nano11010217 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Krstonošić, Veljko
AU  - Đoković, Jelena
AU  - Nikolić, Ines
AU  - Bajuk-Bogdanović, Danica
AU  - Antić-Stanković, Jelena
AU  - Ranđelović, Danijela
AU  - Savić, Sanela M.
AU  - Filipović, Mila
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3581
AB  - Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity
VL  - 15
IS  - 4
DO  - 10.1371/journal.pone.0230993
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Krstonošić, Veljko and Đoković, Jelena and Nikolić, Ines and Bajuk-Bogdanović, Danica and Antić-Stanković, Jelena and Ranđelović, Danijela and Savić, Sanela M. and Filipović, Mila and Tamburić, Slobodanka and Savić, Snežana",
year = "2020",
abstract = "Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity",
volume = "15",
number = "4",
doi = "10.1371/journal.pone.0230993"
}

Gledović, A., Janošević-Ležaić, A., Krstonošić, V., Đoković, J., Nikolić, I., Bajuk-Bogdanović, D., Antić-Stanković, J., Ranđelović, D., Savić, S. M., Filipović, M., Tamburić, S.,& Savić, S.. (2020). Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE
Public Library of Science., 15(4).
https://doi.org/10.1371/journal.pone.0230993

Gledović A, Janošević-Ležaić A, Krstonošić V, Đoković J, Nikolić I, Bajuk-Bogdanović D, Antić-Stanković J, Ranđelović D, Savić SM, Filipović M, Tamburić S, Savić S. Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE. 2020;15(4).
doi:10.1371/journal.pone.0230993 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Krstonošić, Veljko, Đoković, Jelena, Nikolić, Ines, Bajuk-Bogdanović, Danica, Antić-Stanković, Jelena, Ranđelović, Danijela, Savić, Sanela M., Filipović, Mila, Tamburić, Slobodanka, Savić, Snežana, "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity" in PLoS ONE, 15, no. 4 (2020),
https://doi.org/10.1371/journal.pone.0230993 . .

TY  - JOUR
AU  - Dabetić, Nevena
AU  - Todorović, Vanja
AU  - Đuričić, Ivana
AU  - Antić-Stanković, Jelena
AU  - Bašić, Zorica N.
AU  - Vujović, Dragan S.
AU  - Šobajić, Slađana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3608
AB  - The amount of organic pomace, left behind agricultural processes, is continuously rising in accordance with industrial progress. Grape pomace, generated in the wine industry all over the world, represents a raw material for obtaining valuable products. Grape seeds are especially rich in oil containing bioactive compounds that can have various health-related effects. The aim of the study is to compare the quality of seed oils obtained from six white grapes, including two Serbian autochthonous varieties. Linoleic acid, associated with numerous health benefits, is the major fatty acid in all samples (≈66% of total); α-tocopherol is the main tocopherol homologue. Total polyphenol content ranges from 73.4 to 104.3 mg of gallic acid equivalents per 100 g. In order to provide comprehensive information about antioxidant capacity of grape seed oil (GSO), three tests are performed (ferric ion reducing antioxidant power; 2,2’-diphenyl-1-picrylhydrazyl, and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical scavenging). Antimicrobial activity is investigated against different strains; however, GSO inhibits the growth of Staphylococcus aureus and Candida albicans. Obtained results are used to develop a novel approach for oil quality assessment. Calculated oil quality scores (OQS) reveal no significant difference between international and autochthonous varieties, although Smederevka stands out as the most potent one. Practical applications: Considering the progressive waste increase in the wine industry and keeping in mind all health-promoting effects of grape seed oil (GSO), it is clearly observed that oil production represents a profitable and sustainable utilization of grape seeds. The results of the present study show that GSO is a rich source of compounds with antioxidant and antimicrobial properties. In addition, this work provides deeper insight into a quality of Serbian autochthonous grape varieties that are still insufficiently explored. Furthermore, the proposed oil quality score could be used as a comprehensive and unbiased method that enables quality assessment of oils. This tool can find practical application in comparing different plant oils regarding their compositional and functional properties. Finally, it would contribute to making some general oil intake recommendations.
PB  - Wiley-VCH Verlag
T2  - European Journal of Lipid Science and Technology
T1  - Grape Seed Oil Characterization: A Novel Approach for Oil Quality Assessment
VL  - 122
IS  - 6
DO  - DOI: 10.1002/ejlt.201900447
ER  - 

@article{
author = "Dabetić, Nevena and Todorović, Vanja and Đuričić, Ivana and Antić-Stanković, Jelena and Bašić, Zorica N. and Vujović, Dragan S. and Šobajić, Slađana",
year = "2020",
abstract = "The amount of organic pomace, left behind agricultural processes, is continuously rising in accordance with industrial progress. Grape pomace, generated in the wine industry all over the world, represents a raw material for obtaining valuable products. Grape seeds are especially rich in oil containing bioactive compounds that can have various health-related effects. The aim of the study is to compare the quality of seed oils obtained from six white grapes, including two Serbian autochthonous varieties. Linoleic acid, associated with numerous health benefits, is the major fatty acid in all samples (≈66% of total); α-tocopherol is the main tocopherol homologue. Total polyphenol content ranges from 73.4 to 104.3 mg of gallic acid equivalents per 100 g. In order to provide comprehensive information about antioxidant capacity of grape seed oil (GSO), three tests are performed (ferric ion reducing antioxidant power; 2,2’-diphenyl-1-picrylhydrazyl, and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical scavenging). Antimicrobial activity is investigated against different strains; however, GSO inhibits the growth of Staphylococcus aureus and Candida albicans. Obtained results are used to develop a novel approach for oil quality assessment. Calculated oil quality scores (OQS) reveal no significant difference between international and autochthonous varieties, although Smederevka stands out as the most potent one. Practical applications: Considering the progressive waste increase in the wine industry and keeping in mind all health-promoting effects of grape seed oil (GSO), it is clearly observed that oil production represents a profitable and sustainable utilization of grape seeds. The results of the present study show that GSO is a rich source of compounds with antioxidant and antimicrobial properties. In addition, this work provides deeper insight into a quality of Serbian autochthonous grape varieties that are still insufficiently explored. Furthermore, the proposed oil quality score could be used as a comprehensive and unbiased method that enables quality assessment of oils. This tool can find practical application in comparing different plant oils regarding their compositional and functional properties. Finally, it would contribute to making some general oil intake recommendations.",
publisher = "Wiley-VCH Verlag",
journal = "European Journal of Lipid Science and Technology",
title = "Grape Seed Oil Characterization: A Novel Approach for Oil Quality Assessment",
volume = "122",
number = "6",
doi = "DOI: 10.1002/ejlt.201900447"
}

Dabetić, N., Todorović, V., Đuričić, I., Antić-Stanković, J., Bašić, Z. N., Vujović, D. S.,& Šobajić, S.. (2020). Grape Seed Oil Characterization: A Novel Approach for Oil Quality Assessment. in European Journal of Lipid Science and Technology
Wiley-VCH Verlag., 122(6).
https://doi.org/DOI: 10.1002/ejlt.201900447

Dabetić N, Todorović V, Đuričić I, Antić-Stanković J, Bašić ZN, Vujović DS, Šobajić S. Grape Seed Oil Characterization: A Novel Approach for Oil Quality Assessment. in European Journal of Lipid Science and Technology. 2020;122(6).
doi:DOI: 10.1002/ejlt.201900447 .

Dabetić, Nevena, Todorović, Vanja, Đuričić, Ivana, Antić-Stanković, Jelena, Bašić, Zorica N., Vujović, Dragan S., Šobajić, Slađana, "Grape Seed Oil Characterization: A Novel Approach for Oil Quality Assessment" in European Journal of Lipid Science and Technology, 122, no. 6 (2020),
https://doi.org/DOI: 10.1002/ejlt.201900447 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Mitsou, Evgenia
AU  - Damjanović, Ana
AU  - Papadimitriou, Vassiliki
AU  - Antić-Stanković, Jelena
AU  - Stanojević, Boban
AU  - Xenakis, Aristotelis
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3528
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI < 0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity
VL  - 301
DO  - 10.1016/j.molliq.2020.112479
ER  - 

@article{
author = "Nikolić, Ines and Mitsou, Evgenia and Damjanović, Ana and Papadimitriou, Vassiliki and Antić-Stanković, Jelena and Stanojević, Boban and Xenakis, Aristotelis and Savić, Snežana",
year = "2020",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI < 0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity",
volume = "301",
doi = "10.1016/j.molliq.2020.112479"
}

Nikolić, I., Mitsou, E., Damjanović, A., Papadimitriou, V., Antić-Stanković, J., Stanojević, B., Xenakis, A.,& Savić, S.. (2020). Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity. in Journal of Molecular Liquids
Elsevier B.V.., 301.
https://doi.org/10.1016/j.molliq.2020.112479

Nikolić I, Mitsou E, Damjanović A, Papadimitriou V, Antić-Stanković J, Stanojević B, Xenakis A, Savić S. Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity. in Journal of Molecular Liquids. 2020;301.
doi:10.1016/j.molliq.2020.112479 .

Nikolić, Ines, Mitsou, Evgenia, Damjanović, Ana, Papadimitriou, Vassiliki, Antić-Stanković, Jelena, Stanojević, Boban, Xenakis, Aristotelis, Savić, Snežana, "Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity" in Journal of Molecular Liquids, 301 (2020),
https://doi.org/10.1016/j.molliq.2020.112479 . .

TY  - JOUR
AU  - Damjanović, Ana
AU  - Kolundžija, Branka
AU  - Matić, Ivana
AU  - Krivokuća, Ana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Janković, Radmila
AU  - Antić-Stanković, Jelena
AU  - Stanojković, Tatjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3738
AB  - Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.
PB  - MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro
VL  - 25
IS  - 22
DO  - 10.3390/molecules25225233
ER  - 

@article{
author = "Damjanović, Ana and Kolundžija, Branka and Matić, Ivana and Krivokuća, Ana and Zdunić, Gordana and Šavikin, Katarina and Janković, Radmila and Antić-Stanković, Jelena and Stanojković, Tatjana",
year = "2020",
abstract = "Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.",
publisher = "MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro",
volume = "25",
number = "22",
doi = "10.3390/molecules25225233"
}

Damjanović, A., Kolundžija, B., Matić, I., Krivokuća, A., Zdunić, G., Šavikin, K., Janković, R., Antić-Stanković, J.,& Stanojković, T.. (2020). Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland)
MDPI., 25(22).
https://doi.org/10.3390/molecules25225233

Damjanović A, Kolundžija B, Matić I, Krivokuća A, Zdunić G, Šavikin K, Janković R, Antić-Stanković J, Stanojković T. Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland). 2020;25(22).
doi:10.3390/molecules25225233 .

Damjanović, Ana, Kolundžija, Branka, Matić, Ivana, Krivokuća, Ana, Zdunić, Gordana, Šavikin, Katarina, Janković, Radmila, Antić-Stanković, Jelena, Stanojković, Tatjana, "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro" in Molecules (Basel, Switzerland), 25, no. 22 (2020),
https://doi.org/10.3390/molecules25225233 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Ranđelovic, Danijela
AU  - Marković, Bojan
AU  - Lunter, Dominique Jasmin
AU  - Kremenović, Aleksandar
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3711
AB  - As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.
PB  - Walter de Gruyter GmbH
T2  - Reviews on Advanced Materials Science
T1  - Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals
VL  - 59
IS  - 1
SP  - 406
EP  - 424
DO  - 10.1515/rams-2020-0043
DO  - 2-s2.0-85092928784
ER  - 

@article{
author = "Nikolić, Ines and Antić-Stanković, Jelena and Božić, Dragana and Ranđelovic, Danijela and Marković, Bojan and Lunter, Dominique Jasmin and Kremenović, Aleksandar and Savić, Miroslav and Savić, Snežana",
year = "2020",
abstract = "As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.",
publisher = "Walter de Gruyter GmbH",
journal = "Reviews on Advanced Materials Science",
title = "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals",
volume = "59",
number = "1",
pages = "406-424",
doi = "10.1515/rams-2020-0043, 2-s2.0-85092928784"
}

Nikolić, I., Antić-Stanković, J., Božić, D., Ranđelovic, D., Marković, B., Lunter, D. J., Kremenović, A., Savić, M.,& Savić, S.. (2020). Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science
Walter de Gruyter GmbH., 59(1), 406-424.
https://doi.org/10.1515/rams-2020-0043

Nikolić I, Antić-Stanković J, Božić D, Ranđelovic D, Marković B, Lunter DJ, Kremenović A, Savić M, Savić S. Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science. 2020;59(1):406-424.
doi:10.1515/rams-2020-0043 .

Nikolić, Ines, Antić-Stanković, Jelena, Božić, Dragana, Ranđelovic, Danijela, Marković, Bojan, Lunter, Dominique Jasmin, Kremenović, Aleksandar, Savić, Miroslav, Savić, Snežana, "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals" in Reviews on Advanced Materials Science, 59, no. 1 (2020):406-424,
https://doi.org/10.1515/rams-2020-0043 . .

TY  - JOUR
AU  - Antonijević-Nikolić, Mirjana
AU  - Antić-Stanković, Jelena
AU  - Dražić, Branka
AU  - Tanasković, Slađana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3336
AB  - New cationic mixed-ligand tetranuclear complex [Cu-4(tpht) (tpmc)(2)](ClO4)(6)center dot 5H(2)O (tpmc = N, N', N '', N"'-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane, H(2)tpht - terephthalic acid (benzene-1,4-dicarboxylic acid)) was prepared and characterized by elemental analysis (C, H, N, Cu), UV/Vis, IR, EPR (X-band) spectra, conductivity, cyclic voltammetry and magnetic measurements (SQUID). Five coordinated geometry for new Cu(II) complex is proposed. The central metal ion is coordinated with two pyridyl and two cyclam nitrogens and bridged with -N-(CH2)(3)-N- fragments of the cyclam ring and OCO- of the terephthalate anion. Copper (II) ions are coordinated out of cyclam rings and one OCO- group from tpht bridges two Cu(II) inside tpmc unit and two Cu(2)tpmc units are bridged by dicarboxylate tpht dianion engaging all four oxygen atoms. Weak ferromagnetic interaction between copper (II) ions in new tetranuclear complex is observed, Weiss constant theta = 0.21 K and C = 0.398 cm(3) Kmol(-1). The new complex, related Cu(II) complexes with bridged benzene carboxylate, [Cu-2(C6H5COO)tpmc](ClO4)(3)center dot CH3OH, [Cu-2(Hpht)tpmc](ClO4)(3)center dot 3H(2)O (H(2)pht = phthalic acid), [Cu-4(ipht) (tpmc)(2)](ClO4)(6)center dot NaClO4 center dot 2CH(3)CN (H(2)ipht = isophthalic acid), solvents, starting salts and ligands were in vitro evaluated for antiproliferative and antibacterial activity. Antiproliferative evaluation was carried out using tumour cell lines: HeLa (human adenocarcinoma) and K562 (chronic myelogenous leukaemia). Among all tested compounds, the ipht complex revealed the highest antiproliferative activity. According to antibacterial screening results, most active compound was the benzoate complex against Micrococcus lysodeikticus.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Molecular Structure
T1  - New macrocyclic Cu(II) complex with bridge terephthalate: Synthesis, spectral properties, in vitro cytotoxic and antimicrobial activity. Comparison with related complexes
VL  - 1184
SP  - 41
EP  - 48
DO  - 10.1016/j.molstruc.2018.10.027
ER  - 

@article{
author = "Antonijević-Nikolić, Mirjana and Antić-Stanković, Jelena and Dražić, Branka and Tanasković, Slađana",
year = "2019",
abstract = "New cationic mixed-ligand tetranuclear complex [Cu-4(tpht) (tpmc)(2)](ClO4)(6)center dot 5H(2)O (tpmc = N, N', N '', N"'-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane, H(2)tpht - terephthalic acid (benzene-1,4-dicarboxylic acid)) was prepared and characterized by elemental analysis (C, H, N, Cu), UV/Vis, IR, EPR (X-band) spectra, conductivity, cyclic voltammetry and magnetic measurements (SQUID). Five coordinated geometry for new Cu(II) complex is proposed. The central metal ion is coordinated with two pyridyl and two cyclam nitrogens and bridged with -N-(CH2)(3)-N- fragments of the cyclam ring and OCO- of the terephthalate anion. Copper (II) ions are coordinated out of cyclam rings and one OCO- group from tpht bridges two Cu(II) inside tpmc unit and two Cu(2)tpmc units are bridged by dicarboxylate tpht dianion engaging all four oxygen atoms. Weak ferromagnetic interaction between copper (II) ions in new tetranuclear complex is observed, Weiss constant theta = 0.21 K and C = 0.398 cm(3) Kmol(-1). The new complex, related Cu(II) complexes with bridged benzene carboxylate, [Cu-2(C6H5COO)tpmc](ClO4)(3)center dot CH3OH, [Cu-2(Hpht)tpmc](ClO4)(3)center dot 3H(2)O (H(2)pht = phthalic acid), [Cu-4(ipht) (tpmc)(2)](ClO4)(6)center dot NaClO4 center dot 2CH(3)CN (H(2)ipht = isophthalic acid), solvents, starting salts and ligands were in vitro evaluated for antiproliferative and antibacterial activity. Antiproliferative evaluation was carried out using tumour cell lines: HeLa (human adenocarcinoma) and K562 (chronic myelogenous leukaemia). Among all tested compounds, the ipht complex revealed the highest antiproliferative activity. According to antibacterial screening results, most active compound was the benzoate complex against Micrococcus lysodeikticus.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Molecular Structure",
title = "New macrocyclic Cu(II) complex with bridge terephthalate: Synthesis, spectral properties, in vitro cytotoxic and antimicrobial activity. Comparison with related complexes",
volume = "1184",
pages = "41-48",
doi = "10.1016/j.molstruc.2018.10.027"
}

Antonijević-Nikolić, M., Antić-Stanković, J., Dražić, B.,& Tanasković, S.. (2019). New macrocyclic Cu(II) complex with bridge terephthalate: Synthesis, spectral properties, in vitro cytotoxic and antimicrobial activity. Comparison with related complexes. in Journal of Molecular Structure
Elsevier Science BV, Amsterdam., 1184, 41-48.
https://doi.org/10.1016/j.molstruc.2018.10.027

Antonijević-Nikolić M, Antić-Stanković J, Dražić B, Tanasković S. New macrocyclic Cu(II) complex with bridge terephthalate: Synthesis, spectral properties, in vitro cytotoxic and antimicrobial activity. Comparison with related complexes. in Journal of Molecular Structure. 2019;1184:41-48.
doi:10.1016/j.molstruc.2018.10.027 .

Antonijević-Nikolić, Mirjana, Antić-Stanković, Jelena, Dražić, Branka, Tanasković, Slađana, "New macrocyclic Cu(II) complex with bridge terephthalate: Synthesis, spectral properties, in vitro cytotoxic and antimicrobial activity. Comparison with related complexes" in Journal of Molecular Structure, 1184 (2019):41-48,
https://doi.org/10.1016/j.molstruc.2018.10.027 . .

TY  - JOUR
AU  - Antonijević Nikolić, Mirjana
AU  - Dražić, Branka
AU  - Antić-Stanković, Jelena
AU  - Tanasković, Slađana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4379
AB  - New carboxylate complexes of the tetraazamacrocyclic ligand
N,N',N'',N'''-tetrakis(2-pyridilmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc)
with Ni(II) and Zn(II) as central ions were prepared. In mixed-ligand com-
plexes (endo,endo)-bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylate dianion (C9H8O42-
)
is also coordinated to metal ions. The complexes were characterized by ele-
mental analysis (C, H, N), FTIR and UV–Vis spectroscopy, molar conductivity
determination and magnetic susceptibility measurement at room temperature.
The analytical data of the complexes show the formation of binuclear
[Ni2(C9H8O4)tpmc](ClO4)2·4H2O and tetranuclear [Zn4(C9H8O4)(tpmc)2](ClO4)6·
·CH3CN·KClO 4·4H2O complexes. In tetranuclear Zn(II) complex bicyclic
dicarboxylate ligand is most likely to be bridge coordinated, and in binuclear
Ni(II) complex it is coordinated in a combined bridged manner with chelate
rings formation. In both complexes macrocyclic ligand was exo coordinated,
out of cyclam ring and adopts a boat conformation. The Zn(II) complex is one
of the rare tetranuclear Zn(II)-tpmc complexes with carboxylate ion bridging
two Zn2tpmc units. The complexes were tested for antibacterial activity against
Gram-positive bacteria Staphylococcus aureus (ATCC 25923) and Bacillus
subtilis (ATCC 6633), Gram-negative bacterium Escherichia coli (ATCC
25922), and yeast Candida albicans (ATCC 10231), and were screened for
antiproliferative activity against human cervix adenocarcinoma (HeLa) and
human myelogenous leukemia (K562) cell lines.
AB  - Синтетисани су нови карбоксилато комплекси са тетраазамакроцикличним ли-
гандом N,N',N'',N'''-тетракис(2-пиридилметил)-1,4,8,11-тетраазациклотетрадеканом
(tpmc) и Ni(II) и Zn(II) као централним јонима. У мешовитолигандним комплексима
(endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилато дианјон (C9H8O4
2-
) је такође
везан за метални јон. Комплекси су окарактерисани елементалном анализом (C, H, N),
FTIR и UV/Vis спектроскопијом, као и мерењем моларне проводљивости и магнетне
сусцептибилности на собној температури. На основу добијених резултата претпос-
тављена је бинуклеарна структура [Ni2(C9H8O4)tpmc](ClO4) 2·4H2O комплекса, односно,
тетрануклеарна [Zn4(C9H8O4 )(tpmc) 2](ClO4)6·CH3CN·KClO4 ·4H2O комплекса. У тетра-
нуклеарном Zn(II) комплексу, бициклични дикарбоксилато лиганд је највероватније
мостно везан, док је у бинуклеарном Ni(II) комплексу мешовити мостно-хелатни начин
координације. Други начини мостног везивања дикарбоксилата не могу бити у потпуности искључени. У оба комплекса макроциклични лиганд је егзо координован, изван
цикламовог прстена и заузима конформацију лађе. Добијени Zn(II) кoмплекс је један од
ретких тетрануклеарних Zn(II)–tpmc комплекса са карбоксилато лигандом у мосту
између две Zn2tpmc јединице. Добијени комплекси су тестирани на антибактеријску
активност према Грам-позитивним бактеријама Staphylococcus aureus (ATCC 25923) и
Bacillus subtilis (ATCC 6633), Грам-негативној бактерији Escherichia coli (ATCC 25922) и
квасцу Candida albicans (ATCC 10231). Испитивана је њихова антипролиферативна
активност на хуманим малигним ћелијским линијама: цервикалног аденокарцинома
(HeLa) и мијелогене леукемије (K562).
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity
T1  - Nови мешовитолигандни макроциклични комплекси Ni(II) и Zn(II) са мостовним (endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилатом: синтеза, карактеризација, антимикробна и цитотоксична активност
VL  - 84
IS  - 9
SP  - 961
EP  - 973
DO  - https://doi.org/10.2298/JSC181216028A
ER  - 

@article{
author = "Antonijević Nikolić, Mirjana and Dražić, Branka and Antić-Stanković, Jelena and Tanasković, Slađana",
year = "2019",
abstract = "New carboxylate complexes of the tetraazamacrocyclic ligand
N,N',N'',N'''-tetrakis(2-pyridilmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc)
with Ni(II) and Zn(II) as central ions were prepared. In mixed-ligand com-
plexes (endo,endo)-bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylate dianion (C9H8O42-
)
is also coordinated to metal ions. The complexes were characterized by ele-
mental analysis (C, H, N), FTIR and UV–Vis spectroscopy, molar conductivity
determination and magnetic susceptibility measurement at room temperature.
The analytical data of the complexes show the formation of binuclear
[Ni2(C9H8O4)tpmc](ClO4)2·4H2O and tetranuclear [Zn4(C9H8O4)(tpmc)2](ClO4)6·
·CH3CN·KClO 4·4H2O complexes. In tetranuclear Zn(II) complex bicyclic
dicarboxylate ligand is most likely to be bridge coordinated, and in binuclear
Ni(II) complex it is coordinated in a combined bridged manner with chelate
rings formation. In both complexes macrocyclic ligand was exo coordinated,
out of cyclam ring and adopts a boat conformation. The Zn(II) complex is one
of the rare tetranuclear Zn(II)-tpmc complexes with carboxylate ion bridging
two Zn2tpmc units. The complexes were tested for antibacterial activity against
Gram-positive bacteria Staphylococcus aureus (ATCC 25923) and Bacillus
subtilis (ATCC 6633), Gram-negative bacterium Escherichia coli (ATCC
25922), and yeast Candida albicans (ATCC 10231), and were screened for
antiproliferative activity against human cervix adenocarcinoma (HeLa) and
human myelogenous leukemia (K562) cell lines., Синтетисани су нови карбоксилато комплекси са тетраазамакроцикличним ли-
гандом N,N',N'',N'''-тетракис(2-пиридилметил)-1,4,8,11-тетраазациклотетрадеканом
(tpmc) и Ni(II) и Zn(II) као централним јонима. У мешовитолигандним комплексима
(endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилато дианјон (C9H8O4
2-
) је такође
везан за метални јон. Комплекси су окарактерисани елементалном анализом (C, H, N),
FTIR и UV/Vis спектроскопијом, као и мерењем моларне проводљивости и магнетне
сусцептибилности на собној температури. На основу добијених резултата претпос-
тављена је бинуклеарна структура [Ni2(C9H8O4)tpmc](ClO4) 2·4H2O комплекса, односно,
тетрануклеарна [Zn4(C9H8O4 )(tpmc) 2](ClO4)6·CH3CN·KClO4 ·4H2O комплекса. У тетра-
нуклеарном Zn(II) комплексу, бициклични дикарбоксилато лиганд је највероватније
мостно везан, док је у бинуклеарном Ni(II) комплексу мешовити мостно-хелатни начин
координације. Други начини мостног везивања дикарбоксилата не могу бити у потпуности искључени. У оба комплекса макроциклични лиганд је егзо координован, изван
цикламовог прстена и заузима конформацију лађе. Добијени Zn(II) кoмплекс је један од
ретких тетрануклеарних Zn(II)–tpmc комплекса са карбоксилато лигандом у мосту
између две Zn2tpmc јединице. Добијени комплекси су тестирани на антибактеријску
активност према Грам-позитивним бактеријама Staphylococcus aureus (ATCC 25923) и
Bacillus subtilis (ATCC 6633), Грам-негативној бактерији Escherichia coli (ATCC 25922) и
квасцу Candida albicans (ATCC 10231). Испитивана је њихова антипролиферативна
активност на хуманим малигним ћелијским линијама: цервикалног аденокарцинома
(HeLa) и мијелогене леукемије (K562).",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity, Nови мешовитолигандни макроциклични комплекси Ni(II) и Zn(II) са мостовним (endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилатом: синтеза, карактеризација, антимикробна и цитотоксична активност",
volume = "84",
number = "9",
pages = "961-973",
doi = "https://doi.org/10.2298/JSC181216028A"
}

Antonijević Nikolić, M., Dražić, B., Antić-Stanković, J.,& Tanasković, S.. (2019). New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 84(9), 961-973.
https://doi.org/https://doi.org/10.2298/JSC181216028A

Antonijević Nikolić M, Dražić B, Antić-Stanković J, Tanasković S. New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity. in Journal of the Serbian Chemical Society. 2019;84(9):961-973.
doi:https://doi.org/10.2298/JSC181216028A .

Antonijević Nikolić, Mirjana, Dražić, Branka, Antić-Stanković, Jelena, Tanasković, Slađana, "New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity" in Journal of the Serbian Chemical Society, 84, no. 9 (2019):961-973,
https://doi.org/https://doi.org/10.2298/JSC181216028A . .

TY  - JOUR
AU  - Antonijević-Nikolić, Mirjana
AU  - Antić-Stanković, Jelena
AU  - Tanasković, Slađana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3083
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3426
AB  - AbstrcatNew cationic tetranuclear Co(II) and neutral binuclear Cu(II) complexes with tpmc (N,N,N,N-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane) and bridging pyromellitate ligand pma (tetraanion 1,2,4,5-benzenetetracarboxylic acid) were isolated. The composition of the compounds is proposed based on elemental analyses (C, H, N, M=Cu, Co), molar conductivity determination, UV-Vis, FTIR, EPR, LC-MS and reflectance spectroscopy, magnetic measurements, cyclic voltammetry, as well as TG and DTA. It is proposed that in [Co-4(pma)(tpmc)(2)](ClO4)(4)6H(2)O (1), cobalt(II) is six-coordinate out of cyclam rings and one OCO- from pma participates in coordination with one Co(II). In the case of [Cu-2(pma)tpmc].8H(2)O (2), one OCO- from pma bridges two Cu(II). The cytotoxic activity of 1 and 2 was tested against tumor cell lines human cervix adenocarcinoma (HeLa), estrogen-receptor-positive human breast cancer (MCF-7), human myelogenous leukemia (K562), and the human Caucasian Burkitt's lymphoma (Ramos). The IC50 values for 1 and 2 were within the range 44.66 +/- 2.39 to 152.40 +/- 2.28M, and from 140.88 +/- 3.51 to 192.05 +/- 2.09M, respectively. Both 1 and 2 were tested for antimicrobial activity. We determined that minimal inhibitory concentration for 1 against Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae was 25mM. Complex 2 did not express activity against tested microbial strains.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Coordination Chemistry
T1  - Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid
VL  - 71
IS  - 10
SP  - 1542
EP  - 1559
DO  - 10.1080/00958972.2018.1459581
ER  - 

@article{
author = "Antonijević-Nikolić, Mirjana and Antić-Stanković, Jelena and Tanasković, Slađana",
year = "2018",
abstract = "AbstrcatNew cationic tetranuclear Co(II) and neutral binuclear Cu(II) complexes with tpmc (N,N,N,N-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane) and bridging pyromellitate ligand pma (tetraanion 1,2,4,5-benzenetetracarboxylic acid) were isolated. The composition of the compounds is proposed based on elemental analyses (C, H, N, M=Cu, Co), molar conductivity determination, UV-Vis, FTIR, EPR, LC-MS and reflectance spectroscopy, magnetic measurements, cyclic voltammetry, as well as TG and DTA. It is proposed that in [Co-4(pma)(tpmc)(2)](ClO4)(4)6H(2)O (1), cobalt(II) is six-coordinate out of cyclam rings and one OCO- from pma participates in coordination with one Co(II). In the case of [Cu-2(pma)tpmc].8H(2)O (2), one OCO- from pma bridges two Cu(II). The cytotoxic activity of 1 and 2 was tested against tumor cell lines human cervix adenocarcinoma (HeLa), estrogen-receptor-positive human breast cancer (MCF-7), human myelogenous leukemia (K562), and the human Caucasian Burkitt's lymphoma (Ramos). The IC50 values for 1 and 2 were within the range 44.66 +/- 2.39 to 152.40 +/- 2.28M, and from 140.88 +/- 3.51 to 192.05 +/- 2.09M, respectively. Both 1 and 2 were tested for antimicrobial activity. We determined that minimal inhibitory concentration for 1 against Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae was 25mM. Complex 2 did not express activity against tested microbial strains.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Coordination Chemistry",
title = "Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid",
volume = "71",
number = "10",
pages = "1542-1559",
doi = "10.1080/00958972.2018.1459581"
}

Antonijević-Nikolić, M., Antić-Stanković, J.,& Tanasković, S.. (2018). Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid. in Journal of Coordination Chemistry
Taylor & Francis Ltd, Abingdon., 71(10), 1542-1559.
https://doi.org/10.1080/00958972.2018.1459581

Antonijević-Nikolić M, Antić-Stanković J, Tanasković S. Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid. in Journal of Coordination Chemistry. 2018;71(10):1542-1559.
doi:10.1080/00958972.2018.1459581 .

Antonijević-Nikolić, Mirjana, Antić-Stanković, Jelena, Tanasković, Slađana, "Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid" in Journal of Coordination Chemistry, 71, no. 10 (2018):1542-1559,
https://doi.org/10.1080/00958972.2018.1459581 . .

TY  - JOUR
AU  - Antonijević-Nikolić, Mirjana
AU  - Antić-Stanković, Jelena
AU  - Tanasković, Slađana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3083
AB  - AbstrcatNew cationic tetranuclear Co(II) and neutral binuclear Cu(II) complexes with tpmc (N,N,N,N-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane) and bridging pyromellitate ligand pma (tetraanion 1,2,4,5-benzenetetracarboxylic acid) were isolated. The composition of the compounds is proposed based on elemental analyses (C, H, N, M=Cu, Co), molar conductivity determination, UV-Vis, FTIR, EPR, LC-MS and reflectance spectroscopy, magnetic measurements, cyclic voltammetry, as well as TG and DTA. It is proposed that in [Co-4(pma)(tpmc)(2)](ClO4)(4)6H(2)O (1), cobalt(II) is six-coordinate out of cyclam rings and one OCO- from pma participates in coordination with one Co(II). In the case of [Cu-2(pma)tpmc].8H(2)O (2), one OCO- from pma bridges two Cu(II). The cytotoxic activity of 1 and 2 was tested against tumor cell lines human cervix adenocarcinoma (HeLa), estrogen-receptor-positive human breast cancer (MCF-7), human myelogenous leukemia (K562), and the human Caucasian Burkitt's lymphoma (Ramos). The IC50 values for 1 and 2 were within the range 44.66 +/- 2.39 to 152.40 +/- 2.28M, and from 140.88 +/- 3.51 to 192.05 +/- 2.09M, respectively. Both 1 and 2 were tested for antimicrobial activity. We determined that minimal inhibitory concentration for 1 against Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae was 25mM. Complex 2 did not express activity against tested microbial strains.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Coordination Chemistry
T1  - Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid
VL  - 71
IS  - 10
SP  - 1542
EP  - 1559
DO  - 10.1080/00958972.2018.1459581
ER  - 

@article{
author = "Antonijević-Nikolić, Mirjana and Antić-Stanković, Jelena and Tanasković, Slađana",
year = "2018",
abstract = "AbstrcatNew cationic tetranuclear Co(II) and neutral binuclear Cu(II) complexes with tpmc (N,N,N,N-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane) and bridging pyromellitate ligand pma (tetraanion 1,2,4,5-benzenetetracarboxylic acid) were isolated. The composition of the compounds is proposed based on elemental analyses (C, H, N, M=Cu, Co), molar conductivity determination, UV-Vis, FTIR, EPR, LC-MS and reflectance spectroscopy, magnetic measurements, cyclic voltammetry, as well as TG and DTA. It is proposed that in [Co-4(pma)(tpmc)(2)](ClO4)(4)6H(2)O (1), cobalt(II) is six-coordinate out of cyclam rings and one OCO- from pma participates in coordination with one Co(II). In the case of [Cu-2(pma)tpmc].8H(2)O (2), one OCO- from pma bridges two Cu(II). The cytotoxic activity of 1 and 2 was tested against tumor cell lines human cervix adenocarcinoma (HeLa), estrogen-receptor-positive human breast cancer (MCF-7), human myelogenous leukemia (K562), and the human Caucasian Burkitt's lymphoma (Ramos). The IC50 values for 1 and 2 were within the range 44.66 +/- 2.39 to 152.40 +/- 2.28M, and from 140.88 +/- 3.51 to 192.05 +/- 2.09M, respectively. Both 1 and 2 were tested for antimicrobial activity. We determined that minimal inhibitory concentration for 1 against Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae was 25mM. Complex 2 did not express activity against tested microbial strains.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Coordination Chemistry",
title = "Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid",
volume = "71",
number = "10",
pages = "1542-1559",
doi = "10.1080/00958972.2018.1459581"
}

Antonijević-Nikolić, M., Antić-Stanković, J.,& Tanasković, S.. (2018). Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid. in Journal of Coordination Chemistry
Taylor & Francis Ltd, Abingdon., 71(10), 1542-1559.
https://doi.org/10.1080/00958972.2018.1459581

Antonijević-Nikolić M, Antić-Stanković J, Tanasković S. Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid. in Journal of Coordination Chemistry. 2018;71(10):1542-1559.
doi:10.1080/00958972.2018.1459581 .

Antonijević-Nikolić, Mirjana, Antić-Stanković, Jelena, Tanasković, Slađana, "Synthesis, characterization, and in vitro antiproliferative and antibacterial studies of tetraazamacrocyclic complexes of Co(II) and Cu(II) with pyromellitic acid" in Journal of Coordination Chemistry, 71, no. 10 (2018):1542-1559,
https://doi.org/10.1080/00958972.2018.1459581 . .

TY  - JOUR
AU  - Milović, Sanja
AU  - Kundaković, Tatjana
AU  - Macić, Vesna
AU  - Antić-Stanković, Jelena
AU  - Grozdanić, Nada
AU  - Đuričić, Ivana
AU  - Stanković, Ivan
AU  - Stanojković, Tatjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2866
AB  - The aim of the present study was to reveal the potential biological activities of the dichlorometane: methanol (1:1) dry extract (DME) and the nutritional value of selected seaweeds: Cymodocea nodosa, Halimeda tuna, Cystoseira barbata and Codium bursa, collected from the Adriatic coast, Montenegro. We assessed the chemical composition and several biological activities such as: anti-a-glucosidase, antitumour, antimicrobial and antioxidative activity. H. tuna had the best cytotoxic activity against human colon carcinoma cell line, LS174 (IC50 = 17.92 +/- 1.54 mu g/mL). C. nodosa demonstrated strong cytotoxicity against human adenocarcinoma cell line, HeLa (IC50 = 13.28 +/- 0.39 mu g/mL) and human chronic myelogenous leukaemia cell line, K562 (IC50 = 19.64 +/- 1.55 mu g/mL). C. barbata had the best anti a-glucosidase (IC50 = 9.98 +/- 3.34 mu g/mL) and antimicrobial activity (minimal inhibitory concentration of 100 mu g/mL) for Staphylococcus aureus and Bacillus subtilis. C. bursa showed the highest nutritional value (490.4 kcal).
PB  - Soc Stinte Farmaceutice Romania, Bucuresti
T2  - Farmacia
T1  - Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro
VL  - 65
IS  - 5
SP  - 731
EP  - 740
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2866
ER  - 

@article{
author = "Milović, Sanja and Kundaković, Tatjana and Macić, Vesna and Antić-Stanković, Jelena and Grozdanić, Nada and Đuričić, Ivana and Stanković, Ivan and Stanojković, Tatjana",
year = "2017",
abstract = "The aim of the present study was to reveal the potential biological activities of the dichlorometane: methanol (1:1) dry extract (DME) and the nutritional value of selected seaweeds: Cymodocea nodosa, Halimeda tuna, Cystoseira barbata and Codium bursa, collected from the Adriatic coast, Montenegro. We assessed the chemical composition and several biological activities such as: anti-a-glucosidase, antitumour, antimicrobial and antioxidative activity. H. tuna had the best cytotoxic activity against human colon carcinoma cell line, LS174 (IC50 = 17.92 +/- 1.54 mu g/mL). C. nodosa demonstrated strong cytotoxicity against human adenocarcinoma cell line, HeLa (IC50 = 13.28 +/- 0.39 mu g/mL) and human chronic myelogenous leukaemia cell line, K562 (IC50 = 19.64 +/- 1.55 mu g/mL). C. barbata had the best anti a-glucosidase (IC50 = 9.98 +/- 3.34 mu g/mL) and antimicrobial activity (minimal inhibitory concentration of 100 mu g/mL) for Staphylococcus aureus and Bacillus subtilis. C. bursa showed the highest nutritional value (490.4 kcal).",
publisher = "Soc Stinte Farmaceutice Romania, Bucuresti",
journal = "Farmacia",
title = "Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro",
volume = "65",
number = "5",
pages = "731-740",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2866"
}

Milović, S., Kundaković, T., Macić, V., Antić-Stanković, J., Grozdanić, N., Đuričić, I., Stanković, I.,& Stanojković, T.. (2017). Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro. in Farmacia
Soc Stinte Farmaceutice Romania, Bucuresti., 65(5), 731-740.
https://hdl.handle.net/21.15107/rcub_farfar_2866

Milović S, Kundaković T, Macić V, Antić-Stanković J, Grozdanić N, Đuričić I, Stanković I, Stanojković T. Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro. in Farmacia. 2017;65(5):731-740.
https://hdl.handle.net/21.15107/rcub_farfar_2866 .

Milović, Sanja, Kundaković, Tatjana, Macić, Vesna, Antić-Stanković, Jelena, Grozdanić, Nada, Đuričić, Ivana, Stanković, Ivan, Stanojković, Tatjana, "Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro" in Farmacia, 65, no. 5 (2017):731-740,
https://hdl.handle.net/21.15107/rcub_farfar_2866 .

TY  - JOUR
AU  - Antić-Stanković, Jelena
AU  - Stanković, Sanja
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2855
AB  - The complement system plays an important role in host defense. It is composed of series of plasma proteins which, when activated in a sequential reaction, unleashes a powerful destructive activity towards invading pathogens. Three major pathways exist in the activation of complement: the classical pathway, which is initiated by antibody-antigen binding, the alternative pathway, which is activated by a susceptible foreign surface, and the lectin pathways, which can be triggered by manann-binding lectins. The complement system consists of serum and cell surface proteins that interact with one another and with other molecules of the immune system in a highly regulated manner. Complement proteins are plasma proteins that are normally inactive and they are activated only under particular conditions to generate products that mediate various effector functions of the complement.
AB  - Sistem komplementa ima značajnu ulogu u odbrani domaćina od patogena. Sastoji se od niza proteina plazme koji se aktiviraju u kaskadnom procesu, dovodeći do oštećenja patogena. Opisana su tri puta aktivacije sistema komplementa: klasičan (aktivira se vezivanjem C1q komponente komplementa za kompleks antigen-antitelo), alternativni (aktivira se direktnim vezivanjem C3b komponente komplementa za površinske molekule mikroorganizama) i lektinski (indukovan je vezivanjem manoza vezućeg proteina za manozne rezidue na površini mikroorganizama). Najvažnija uloga sistema komplementa u urođenom i specifičnom imunitetu je stimulisanje fagocitoze, liza mikroorganizama i pospešivanje inflamacije. Komplement ima ključnu ulogu i u uklanjanju imunskih kompleksa.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta medica Medianae
T1  - The complement system: Pathways of activations and functions
T1  - Sistem komplementa - putevi aktivacije i funkcija
VL  - 56
IS  - 1
SP  - 50
EP  - 55
DO  - 10.5633/amm.2017.0108
ER  - 

@article{
author = "Antić-Stanković, Jelena and Stanković, Sanja",
year = "2017",
abstract = "The complement system plays an important role in host defense. It is composed of series of plasma proteins which, when activated in a sequential reaction, unleashes a powerful destructive activity towards invading pathogens. Three major pathways exist in the activation of complement: the classical pathway, which is initiated by antibody-antigen binding, the alternative pathway, which is activated by a susceptible foreign surface, and the lectin pathways, which can be triggered by manann-binding lectins. The complement system consists of serum and cell surface proteins that interact with one another and with other molecules of the immune system in a highly regulated manner. Complement proteins are plasma proteins that are normally inactive and they are activated only under particular conditions to generate products that mediate various effector functions of the complement., Sistem komplementa ima značajnu ulogu u odbrani domaćina od patogena. Sastoji se od niza proteina plazme koji se aktiviraju u kaskadnom procesu, dovodeći do oštećenja patogena. Opisana su tri puta aktivacije sistema komplementa: klasičan (aktivira se vezivanjem C1q komponente komplementa za kompleks antigen-antitelo), alternativni (aktivira se direktnim vezivanjem C3b komponente komplementa za površinske molekule mikroorganizama) i lektinski (indukovan je vezivanjem manoza vezućeg proteina za manozne rezidue na površini mikroorganizama). Najvažnija uloga sistema komplementa u urođenom i specifičnom imunitetu je stimulisanje fagocitoze, liza mikroorganizama i pospešivanje inflamacije. Komplement ima ključnu ulogu i u uklanjanju imunskih kompleksa.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta medica Medianae",
title = "The complement system: Pathways of activations and functions, Sistem komplementa - putevi aktivacije i funkcija",
volume = "56",
number = "1",
pages = "50-55",
doi = "10.5633/amm.2017.0108"
}

Antić-Stanković, J.,& Stanković, S.. (2017). The complement system: Pathways of activations and functions. in Acta medica Medianae
Univerzitet u Nišu - Medicinski fakultet, Niš., 56(1), 50-55.
https://doi.org/10.5633/amm.2017.0108

Antić-Stanković J, Stanković S. The complement system: Pathways of activations and functions. in Acta medica Medianae. 2017;56(1):50-55.
doi:10.5633/amm.2017.0108 .

Antić-Stanković, Jelena, Stanković, Sanja, "The complement system: Pathways of activations and functions" in Acta medica Medianae, 56, no. 1 (2017):50-55,
https://doi.org/10.5633/amm.2017.0108 . .

TY  - JOUR
AU  - Simić, Milena
AU  - Damjanović, Ana B.
AU  - Kalinić, Marko
AU  - Tasić, Gordana
AU  - Erić, Slavica
AU  - Antić-Stanković, Jelena
AU  - Savić, Vladimir
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2606
AB  - A novel and efficient synthetic route was developed for the preparation of protoberberine derivatives. A methodology, designed primarily to control the substitution patterns on the terminal rings, was used to access a small array of these compounds. An initial biological profiling suggested an anticancer potential of the synthesised derivatives, while structure-based target fishing identified their potential targets and established a rational basis for further structural modifications. Although the activities need further improvement, the study demonstrated that the described approach may be useful in the discovery of novel lead compounds.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis, cytotoxicity and computational study of novel protoberberine derivatives
VL  - 81
IS  - 2
SP  - 103
EP  - 123
DO  - 10.2298/JSC150525090S
ER  - 

@article{
author = "Simić, Milena and Damjanović, Ana B. and Kalinić, Marko and Tasić, Gordana and Erić, Slavica and Antić-Stanković, Jelena and Savić, Vladimir",
year = "2016",
abstract = "A novel and efficient synthetic route was developed for the preparation of protoberberine derivatives. A methodology, designed primarily to control the substitution patterns on the terminal rings, was used to access a small array of these compounds. An initial biological profiling suggested an anticancer potential of the synthesised derivatives, while structure-based target fishing identified their potential targets and established a rational basis for further structural modifications. Although the activities need further improvement, the study demonstrated that the described approach may be useful in the discovery of novel lead compounds.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis, cytotoxicity and computational study of novel protoberberine derivatives",
volume = "81",
number = "2",
pages = "103-123",
doi = "10.2298/JSC150525090S"
}

Simić, M., Damjanović, A. B., Kalinić, M., Tasić, G., Erić, S., Antić-Stanković, J.,& Savić, V.. (2016). Synthesis, cytotoxicity and computational study of novel protoberberine derivatives. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 81(2), 103-123.
https://doi.org/10.2298/JSC150525090S

Simić M, Damjanović AB, Kalinić M, Tasić G, Erić S, Antić-Stanković J, Savić V. Synthesis, cytotoxicity and computational study of novel protoberberine derivatives. in Journal of the Serbian Chemical Society. 2016;81(2):103-123.
doi:10.2298/JSC150525090S .

Simić, Milena, Damjanović, Ana B., Kalinić, Marko, Tasić, Gordana, Erić, Slavica, Antić-Stanković, Jelena, Savić, Vladimir, "Synthesis, cytotoxicity and computational study of novel protoberberine derivatives" in Journal of the Serbian Chemical Society, 81, no. 2 (2016):103-123,
https://doi.org/10.2298/JSC150525090S . .

TY  - JOUR
AU  - Antić-Stanković, Jelena
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2519
AB  - Zoonosis is any disease or infection that is naturally transmissible from animals to human and vice-versa. Zoonoses are an important issue of many countries in the world, and more than 200 has been recognized by now. Pathogenic agents that cause zoonoses are parasites, bacteria, fungi, viruses and prions. Trichinella spiralis is a cylindric parasite that causes trichinellosis. Trichinellosis is a disease that is transmitted by ingestion of meat contaminated by larva. Treatment is often with no success, but prevention and cooperation between veterinary and human medical sector plays an important role in saving human health.
AB  - Sve bolesti ili infekcije koje se prirodnim putem mogu preneti sa životinja na čoveka i obrnuto svrstavamo u zoonoze. Zoonoze su problem u brojnim državama, a do sada je opisano više od 200 ovakvih bolesti. Jedna od klasifikacija zoonoza je na osnovu uzročnika bolesti koji može biti parazit, bakterija, virus, gljivica ili prion. Trichinella spiralis je parazit valjkastog oblika koji izaziva trihinelozu. Ovo je bolest koja se prenosi ingestijom mesa koje sadrži larve trihinele. Bolest je moguće lečiti, ali primarno se radi na prevenciji trihineloze. Lečenje često nije uspešno, ali prevencija i saradnja veterinarskog sektora i sektora humane medicine igra značajnu ulogu u očuvanju zdravlja ljudi.
PB  - Društvo za ishranu Srbije, Beograd
T2  - Hrana i ishrana
T1  - Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention
T1  - Trihineloza - karakteristike uzročnika, način prenošenja i mogućnost prevencije
VL  - 56
IS  - 1
SP  - 12
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2519
ER  - 

@article{
author = "Antić-Stanković, Jelena",
year = "2015",
abstract = "Zoonosis is any disease or infection that is naturally transmissible from animals to human and vice-versa. Zoonoses are an important issue of many countries in the world, and more than 200 has been recognized by now. Pathogenic agents that cause zoonoses are parasites, bacteria, fungi, viruses and prions. Trichinella spiralis is a cylindric parasite that causes trichinellosis. Trichinellosis is a disease that is transmitted by ingestion of meat contaminated by larva. Treatment is often with no success, but prevention and cooperation between veterinary and human medical sector plays an important role in saving human health., Sve bolesti ili infekcije koje se prirodnim putem mogu preneti sa životinja na čoveka i obrnuto svrstavamo u zoonoze. Zoonoze su problem u brojnim državama, a do sada je opisano više od 200 ovakvih bolesti. Jedna od klasifikacija zoonoza je na osnovu uzročnika bolesti koji može biti parazit, bakterija, virus, gljivica ili prion. Trichinella spiralis je parazit valjkastog oblika koji izaziva trihinelozu. Ovo je bolest koja se prenosi ingestijom mesa koje sadrži larve trihinele. Bolest je moguće lečiti, ali primarno se radi na prevenciji trihineloze. Lečenje često nije uspešno, ali prevencija i saradnja veterinarskog sektora i sektora humane medicine igra značajnu ulogu u očuvanju zdravlja ljudi.",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention, Trihineloza - karakteristike uzročnika, način prenošenja i mogućnost prevencije",
volume = "56",
number = "1",
pages = "12-15",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2519"
}

Antić-Stanković, J.. (2015). Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention. in Hrana i ishrana
Društvo za ishranu Srbije, Beograd., 56(1), 12-15.
https://hdl.handle.net/21.15107/rcub_farfar_2519

Antić-Stanković J. Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention. in Hrana i ishrana. 2015;56(1):12-15.
https://hdl.handle.net/21.15107/rcub_farfar_2519 .

Antić-Stanković, Jelena, "Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention" in Hrana i ishrana, 56, no. 1 (2015):12-15,
https://hdl.handle.net/21.15107/rcub_farfar_2519 .