TY  - CONF
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brankov, Jovan
AU  - Zhou, Wei
AU  - Brey, Eric
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5608
AB  - INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.
PB  - Savez hemijskih inženjera, Beograd
C3  - Hemijska industrija
T1  - Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay
VL  - 78
IS  - 1S
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5608
ER  - 

@conference{
author = "Živković, Lada and Spremo-Potparević, Biljana and Bajić, Vladan and Brankov, Jovan and Zhou, Wei and Brey, Eric",
year = "2024",
abstract = "INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay",
volume = "78",
number = "1S",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5608"
}

Živković, L., Spremo-Potparević, B., Bajić, V., Brankov, J., Zhou, W.,& Brey, E.. (2024). Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 78(1S), 14-14.
https://doi.org/10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608

Živković L, Spremo-Potparević B, Bajić V, Brankov J, Zhou W, Brey E. Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija. 2024;78(1S):14-14.
doi:10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

Živković, Lada, Spremo-Potparević, Biljana, Bajić, Vladan, Brankov, Jovan, Zhou, Wei, Brey, Eric, "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay" in Hemijska industrija, 78, no. 1S (2024):14-14,
https://doi.org/10.1016/j.reprotox.2024.108585 .,
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Želježić, Davor
AU  - Bajić, Vladan
AU  - Popović, Petar
AU  - Živković, Lada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5471
AB  - Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.
PB  - Macedonian Academy of Sciences and Arts
C3  - 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
T1  - New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease
VL  - 26
IS  - Supplement
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5471
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Želježić, Davor and Bajić, Vladan and Popović, Petar and Živković, Lada",
year = "2023",
abstract = "Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK",
title = "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease",
volume = "26",
number = "Supplement",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5471"
}

Spremo-Potparević, B., Želježić, D., Bajić, V., Popović, P.,& Živković, L.. (2023). New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
Macedonian Academy of Sciences and Arts., 26(Supplement), 88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471

Spremo-Potparević B, Želježić D, Bajić V, Popović P, Živković L. New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK. 2023;26(Supplement):88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471 .

Spremo-Potparević, Biljana, Želježić, Davor, Bajić, Vladan, Popović, Petar, Živković, Lada, "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease" in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK, 26, no. Supplement (2023):88-88,
https://hdl.handle.net/21.15107/rcub_farfar_5471 .

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5185
AB  - Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.
PB  - Macedonian Academy of Sciences and Arts
C3  - Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
T1  - Evaluation of the antioxidant potential of Biochaga in vitro
VL  - 26
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5185
ER  - 

@conference{
author = "Živković, Lada and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)",
title = "Evaluation of the antioxidant potential of Biochaga in vitro",
volume = "26",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5185"
}

Živković, L., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
Macedonian Academy of Sciences and Arts., 26, 146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185

Živković L, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book). 2023;26:146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185 .

Živković, Lada, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of the antioxidant potential of Biochaga in vitro" in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book), 26 (2023):146-146,
https://hdl.handle.net/21.15107/rcub_farfar_5185 .

TY  - CONF
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4748
AB  - Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist
SP  - 46
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4748
ER  - 

@conference{
author = "Živković, Lada and Borozan, Sunčica and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4748"
}

Živković, L., Borozan, S., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748

Živković L, Borozan S, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748 .

Živković, Lada, Borozan, Sunčica, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):46-46,
https://hdl.handle.net/21.15107/rcub_farfar_4748 .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Salhi, Adil
AU  - Lakota, Katja
AU  - Radovanović, Aleksandar
AU  - Razali, Rozaimi
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Uludag, Mahmut
AU  - Tifratene, Faroug
AU  - Motwalli, Olaa
AU  - Marchand, Benoit
AU  - Bajić, Vladimir
AU  - Gojobori, Takashi
AU  - Isenović, Esma
AU  - Essack, Magbubah
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4249
AB  - More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases
VL  - 17
IS  - 7
DO  - 10.1371/journal.pone.0271737
ER  - 

@article{
author = "Bajić, Vladan and Salhi, Adil and Lakota, Katja and Radovanović, Aleksandar and Razali, Rozaimi and Živković, Lada and Spremo-Potparević, Biljana and Uludag, Mahmut and Tifratene, Faroug and Motwalli, Olaa and Marchand, Benoit and Bajić, Vladimir and Gojobori, Takashi and Isenović, Esma and Essack, Magbubah",
year = "2022",
abstract = "More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases",
volume = "17",
number = "7",
doi = "10.1371/journal.pone.0271737"
}

Bajić, V., Salhi, A., Lakota, K., Radovanović, A., Razali, R., Živković, L., Spremo-Potparević, B., Uludag, M., Tifratene, F., Motwalli, O., Marchand, B., Bajić, V., Gojobori, T., Isenović, E.,& Essack, M.. (2022). DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases. in PLoS ONE
Public Library of Science., 17(7).
https://doi.org/10.1371/journal.pone.0271737

Bajić V, Salhi A, Lakota K, Radovanović A, Razali R, Živković L, Spremo-Potparević B, Uludag M, Tifratene F, Motwalli O, Marchand B, Bajić V, Gojobori T, Isenović E, Essack M. DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases. in PLoS ONE. 2022;17(7).
doi:10.1371/journal.pone.0271737 .

Bajić, Vladan, Salhi, Adil, Lakota, Katja, Radovanović, Aleksandar, Razali, Rozaimi, Živković, Lada, Spremo-Potparević, Biljana, Uludag, Mahmut, Tifratene, Faroug, Motwalli, Olaa, Marchand, Benoit, Bajić, Vladimir, Gojobori, Takashi, Isenović, Esma, Essack, Magbubah, "DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases" in PLoS ONE, 17, no. 7 (2022),
https://doi.org/10.1371/journal.pone.0271737 . .

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Dimitrijević Srećković, Vesna
AU  - Pirković, Andrea
AU  - Petrović, Hristina
AU  - Spremo-Potparević, Biljana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4630
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro
SP  - 94
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4630
ER  - 

@conference{
author = "Živković, Lada and Topalović, Dijana and Borozan, Sunčica and Bajić, Vladan and Dimitrijević Srećković, Vesna and Pirković, Andrea and Petrović, Hristina and Spremo-Potparević, Biljana",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro",
pages = "94-94",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4630"
}

Živković, L., Topalović, D., Borozan, S., Bajić, V., Dimitrijević Srećković, V., Pirković, A., Petrović, H.,& Spremo-Potparević, B.. (2022). Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630

Živković L, Topalović D, Borozan S, Bajić V, Dimitrijević Srećković V, Pirković A, Petrović H, Spremo-Potparević B. Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

Živković, Lada, Topalović, Dijana, Borozan, Sunčica, Bajić, Vladan, Dimitrijević Srećković, Vesna, Pirković, Andrea, Petrović, Hristina, Spremo-Potparević, Biljana, "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):94-94,
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920
AB  - Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
AB  - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
T1  - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
VL  - 71
IS  - 3
SP  - 197
EP  - 206
DO  - 10.5937/arhfarm71‐31060
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada",
year = "2021",
abstract = "Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka",
volume = "71",
number = "3",
pages = "197-206",
doi = "10.5937/arhfarm71‐31060"
}

Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206.
https://doi.org/10.5937/arhfarm71‐31060

Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206.
doi:10.5937/arhfarm71‐31060 .

Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206,
https://doi.org/10.5937/arhfarm71‐31060 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes-Hernández, Tamara
AU  - Zlatković-Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
PB  - Wiley
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.",
publisher = "Wiley",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry
Wiley., 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3900
AB  - Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay
T1  - Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay, Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis
Serbian Medical Society., 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798

Topalović D, Živković L, Đelić N, Bajić V, Spremo-Potparević B. Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay" in Medicinski Casopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

TY  - JOUR
AU  - Bajić, Vladan. P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3554
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.
PB  - Frontiers Media S.A.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 

@article{
author = "Bajić, Vladan. P. and Essack, Magbubah and Živković, Lada and Stewart, Alan and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}

Bajić, Vladan. P., Essack, M., Živković, L., Stewart, A., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E.,& Spremo-Potparević, B.. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fgene.2019.01368

Bajić VP, Essack M, Živković L, Stewart A, Zafirović S, Bajić VB, Gojobori T, Isenović E, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics. 2020;10.
doi:10.3389/fgene.2019.01368 .

Bajić, Vladan. P., Essack, Magbubah, Živković, Lada, Stewart, Alan, Zafirović, Sonja, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma, Spremo-Potparević, Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" in Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - CONF
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5187
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Turkish Society of Toxicology
C3  - 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
T1  - Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5187
ER  - 

@conference{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Turkish Society of Toxicology",
journal = "10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book",
title = "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5187"
}

Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
Turkish Society of Toxicology., 169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187

Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187 .

Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC" in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book (2019):169-169,
https://hdl.handle.net/21.15107/rcub_farfar_5187 .

TY  - CONF
AU  - Bruić, Marija
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5151
AB  - Inonotus obliquus, edible medicinal mushroom found in northern hemisphere, is widely used for the prevention and treatment of cancer, diabetes, colitis and asthma. Its bioactives, triterpens, polysaccharides and polyphenols exhibit antitumor, anti-inflamatory, hypoglycemic, immunomodulatory effects, as well as antioxidative properties. Oxidative stress and increased ROS levels lead to tissue damage, with DNA molecule being particularly susceptible to oxidative damage. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow
T1  - Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro
SP  - 54
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5151
ER  - 

@conference{
author = "Bruić, Marija and Živković, Lada and Bajić, Vladan and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Inonotus obliquus, edible medicinal mushroom found in northern hemisphere, is widely used for the prevention and treatment of cancer, diabetes, colitis and asthma. Its bioactives, triterpens, polysaccharides and polyphenols exhibit antitumor, anti-inflamatory, hypoglycemic, immunomodulatory effects, as well as antioxidative properties. Oxidative stress and increased ROS levels lead to tissue damage, with DNA molecule being particularly susceptible to oxidative damage. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow",
title = "Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro",
pages = "54-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5151"
}

Bruić, M., Živković, L., Bajić, V., Topalović, D.,& Spremo-Potparević, B.. (2019). Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro. in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow
International Comet Assay Working Group (ICAWG)., 54-54.
https://hdl.handle.net/21.15107/rcub_farfar_5151

Bruić M, Živković L, Bajić V, Topalović D, Spremo-Potparević B. Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro. in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow. 2019;:54-54.
https://hdl.handle.net/21.15107/rcub_farfar_5151 .

Bruić, Marija, Živković, Lada, Bajić, Vladan, Topalović, Dijana, Spremo-Potparević, Biljana, "Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro" in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow (2019):54-54,
https://hdl.handle.net/21.15107/rcub_farfar_5151 .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3595
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1- picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078–2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078–10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet „skevindžer“ aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) – skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078–2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078–10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Evaluation of antioxidant potential of cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Bajić, Vladan and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1- picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078–2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078–10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet „skevindžer“ aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) – skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078–2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078–10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Evaluation of antioxidant potential of cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}

Živković, L., Borozan, S., Bajić, V., Đorđević, S., Hristov, A.,& Spremo-Potparević, B.. (2019). Evaluation of antioxidant potential of cordyceps sinensis in vitro. in Medicinski Casopis
Serbian Medical Society., 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450

Živković L, Borozan S, Bajić V, Đorđević S, Hristov A, Spremo-Potparević B. Evaluation of antioxidant potential of cordyceps sinensis in vitro. in Medicinski Casopis. 2019;53(4):129-134.
doi:10.5937/mckg53-24450 .

Živković, Lada, Borozan, Sunčica, Bajić, Vladan, Đorđević, Stefana, Hristov, Aleksandar, Spremo-Potparević, Biljana, "Evaluation of antioxidant potential of cordyceps sinensis in vitro" in Medicinski Casopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3484
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Hindawi
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
DO  - 10.1155/2019/5039372
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Hindawi",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
doi = "10.1155/2019/5039372"
}

Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi., 2019.
https://doi.org/10.1155/2019/5039372

Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/5039372 .

Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/5039372 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3459
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3456
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Lazić, Vesna
AU  - Vukoje, Ivana
AU  - Milicević, Bojana
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan M.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3257
AB  - The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis
VL  - 84
IS  - 6
SP  - 539
EP  - 553
DO  - 10.2298/JSC181217017L
ER  - 

@article{
author = "Lazić, Vesna and Vukoje, Ivana and Milicević, Bojana and Potparević, Biljana and Živković, Lada and Topalović, Dijana and Bajić, Vladan and Sredojević, Dušan and Nedeljković, Jovan M.",
year = "2019",
abstract = "The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis",
volume = "84",
number = "6",
pages = "539-553",
doi = "10.2298/JSC181217017L"
}

Lazić, V., Vukoje, I., Milicević, B., Potparević, B., Živković, L., Topalović, D., Bajić, V., Sredojević, D.,& Nedeljković, J. M.. (2019). Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(6), 539-553.
https://doi.org/10.2298/JSC181217017L

Lazić V, Vukoje I, Milicević B, Potparević B, Živković L, Topalović D, Bajić V, Sredojević D, Nedeljković JM. Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society. 2019;84(6):539-553.
doi:10.2298/JSC181217017L .

Lazić, Vesna, Vukoje, Ivana, Milicević, Bojana, Potparević, Biljana, Živković, Lada, Topalović, Dijana, Bajić, Vladan, Sredojević, Dušan, Nedeljković, Jovan M., "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis" in Journal of the Serbian Chemical Society, 84, no. 6 (2019):539-553,
https://doi.org/10.2298/JSC181217017L . .

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3165
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 

@article{
author = "Dekanski, Dragana and Potparević, Biljana and Bajić, Vladan and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna and Nedeljković, Jovan",
year = "2018",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}

Dekanski, D., Potparević, B., Bajić, V., Živković, L., Topalović, D., Sredojević, D., Lazić, V.,& Nedeljković, J.. (2018). Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064

Dekanski D, Potparević B, Bajić V, Živković L, Topalović D, Sredojević D, Lazić V, Nedeljković J. Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology. 2018;115:42-48.
doi:10.1016/j.fct.2018.02.064 .

Dekanski, Dragana, Potparević, Biljana, Bajić, Vladan, Živković, Lada, Topalović, Dijana, Sredojević, Dušan, Lazić, Vesna, Nedeljković, Jovan, "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid" in Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 . .

TY  - CONF
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5150
AB  - Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...
PB  - National Health Institute Dr. Ricardo Jorge
PB  - Institute of Public Health of the University of Porto (ISPUP)
C3  - 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
T1  - Evaluation of adrenaline-induced DNA damage in vitro by comet assay
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5150
ER  - 

@conference{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...",
publisher = "National Health Institute Dr. Ricardo Jorge, Institute of Public Health of the University of Porto (ISPUP)",
journal = "4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book",
title = "Evaluation of adrenaline-induced DNA damage in vitro by comet assay",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5150"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
National Health Institute Dr. Ricardo Jorge., 135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150

Topalović D, Dekanski D, Spremo-Potparević B, Đelić N, Bajić V, Živković L. Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book. 2018;:135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Evaluation of adrenaline-induced DNA damage in vitro by comet assay" in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book (2018):135-135,
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3131
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Potparević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3092
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Dekanski, Dragana and Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V., Dekanski, D., Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić V, Dekanski D, Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan, Dekanski, Dragana, Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - CONF
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Žukovec, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5186
AB  - Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5186
ER  - 

@conference{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Žukovec, Dijana and Dekanski, Dragana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5186"
}

Čabarkapa-Pirković, A., Živković, L., Žukovec, D., Dekanski, D., Bajić, V.,& Spremo-Potparević, B.. (2017). Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186

Čabarkapa-Pirković A, Živković L, Žukovec D, Dekanski D, Bajić V, Spremo-Potparević B. Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Žukovec, Dijana, Dekanski, Dragana, Bajić, Vladan, Spremo-Potparević, Biljana, "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):110-110,
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

TY  - CONF
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Borozan, Sunčica
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5127
AB  - Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5127
ER  - 

@conference{
author = "Topalović, Dijana and Živković, Lada and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Bajić, Vladan and Borozan, Sunčica and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5127"
}

Topalović, D., Živković, L., Čabarkapa-Pirković, A., Dekanski, D., Bajić, V., Borozan, S.,& Spremo-Potparević, B.. (2017). Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127

Topalović D, Živković L, Čabarkapa-Pirković A, Dekanski D, Bajić V, Borozan S, Spremo-Potparević B. Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127 .

Topalović, Dijana, Živković, Lada, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Bajić, Vladan, Borozan, Sunčica, Spremo-Potparević, Biljana, "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):58-58,
https://hdl.handle.net/21.15107/rcub_farfar_5127 .