TY  - JOUR
AU  - Radović, Jelena
AU  - Leković, Aleksandra
AU  - Damjanović, Ana B.
AU  - Kopanja, Đurđica
AU  - Dodevska, Margarita
AU  - Stanojković, Tatjana
AU  - Marinković, Tijana
AU  - Jelić, Čedomir
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5057
AB  - The composition of biologically active compounds of Calocybe gambosa (Lyophyllaceae) was analysed, and
the antioxidant and cytotoxic activities were tested in vitro. C. gambosa was low in energy, fat, and car-
bohydrates, but rich in proteins and fibres. The total polyphenol content in the extracts was low (23.08 ±
0.67 in aqueous and 24.99 ± 4.25 mg GA mg 1 of extract in methanolic extract). The methanolic extract
showed anti-DPPH radical activity with an IC 50 of 626.10 ± 25.20 mg mL 1 . The sample of C. gambosa is
rich in nucleotides and amino acids responsible for its pleasant taste. The nucleoside and 50-mono-
phosphates contents were 0.97 mg g 1 and 2.32 mg g 1 of dry mushroom, respectively. The contents of
essential and non-essential amino acids were 18.41 ± 0.06 and 41.75 ± 0.30 mg g 1 d.w., respectively. The
percentages of saturated and unsaturated fatty acids were 42.6% and 57.4%, respectively. The most
abundant water-soluble and fat-soluble vitamins were B1 and E, respectively. Cytotoxic effect of the extracts was examined against different cancer cell lines, and the best cytotoxicity was showed by the
peptide extract against colon cancer cell line LS174.
T2  - Acta Alimentaria
T1  - St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds
VL  - 51
IS  - 1
SP  - 134
EP  - 144
DO  - 10.1556/066.2021.00227
ER  - 

@article{
author = "Radović, Jelena and Leković, Aleksandra and Damjanović, Ana B. and Kopanja, Đurđica and Dodevska, Margarita and Stanojković, Tatjana and Marinković, Tijana and Jelić, Čedomir and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "The composition of biologically active compounds of Calocybe gambosa (Lyophyllaceae) was analysed, and
the antioxidant and cytotoxic activities were tested in vitro. C. gambosa was low in energy, fat, and car-
bohydrates, but rich in proteins and fibres. The total polyphenol content in the extracts was low (23.08 ±
0.67 in aqueous and 24.99 ± 4.25 mg GA mg 1 of extract in methanolic extract). The methanolic extract
showed anti-DPPH radical activity with an IC 50 of 626.10 ± 25.20 mg mL 1 . The sample of C. gambosa is
rich in nucleotides and amino acids responsible for its pleasant taste. The nucleoside and 50-mono-
phosphates contents were 0.97 mg g 1 and 2.32 mg g 1 of dry mushroom, respectively. The contents of
essential and non-essential amino acids were 18.41 ± 0.06 and 41.75 ± 0.30 mg g 1 d.w., respectively. The
percentages of saturated and unsaturated fatty acids were 42.6% and 57.4%, respectively. The most
abundant water-soluble and fat-soluble vitamins were B1 and E, respectively. Cytotoxic effect of the extracts was examined against different cancer cell lines, and the best cytotoxicity was showed by the
peptide extract against colon cancer cell line LS174.",
journal = "Acta Alimentaria",
title = "St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds",
volume = "51",
number = "1",
pages = "134-144",
doi = "10.1556/066.2021.00227"
}

Radović, J., Leković, A., Damjanović, A. B., Kopanja, Đ., Dodevska, M., Stanojković, T., Marinković, T., Jelić, Č.,& Kundaković-Vasović, T.. (2022). St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds. in Acta Alimentaria, 51(1), 134-144.
https://doi.org/10.1556/066.2021.00227

Radović J, Leković A, Damjanović AB, Kopanja Đ, Dodevska M, Stanojković T, Marinković T, Jelić Č, Kundaković-Vasović T. St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds. in Acta Alimentaria. 2022;51(1):134-144.
doi:10.1556/066.2021.00227 .

Radović, Jelena, Leković, Aleksandra, Damjanović, Ana B., Kopanja, Đurđica, Dodevska, Margarita, Stanojković, Tatjana, Marinković, Tijana, Jelić, Čedomir, Kundaković-Vasović, Tatjana, "St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds" in Acta Alimentaria, 51, no. 1 (2022):134-144,
https://doi.org/10.1556/066.2021.00227 . .

TY  - CONF
AU  - Damjanović, Ana
AU  - Vuković, Miodrag
AU  - Vukadinović, Dragana
AU  - Dobričić, Vladimir
AU  - Grahovac, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5450
AB  - Melanoma is one of the most aggressive malignancies, where the prognosis for metastatic patients remains extremely poor. Our group has shown that the antihypertensive drug telmisartan has antimelanoma potential1. Given that the antihypertensive effect is not favorable in cancer patients, the aim of this study was to design and test novel telmisartan derivatives without the angiotensin receptor 1 (AT1R) binding activity. New derivatives were designed by modification of the carboxylic group, in order to alter telmisartan geometry and its AT1R binding properties. Eight derivatives, from which the lack of AT1R antagonistic activity could be expected based on molecular docking, were synthetized and selected for in vitro testing. After the cytotoxicity test on human melanoma cell lines A375 and 518a2, three derivatives that were twice more potent than telmisartan itself were selected for further analysis. The new derivatives induced mitochondrial fragmentation, generation of the mitochondrial reactive oxygen species, and decrease of mitochondrial membrane potential in melanoma cells, the mechanism we previously shown for induction of apoptosis by telmisartan in melanoma cells. As the new derivatives showed more potent effect on melanoma cells than telmisartan these results lay a ground for further preclinical testing in melanoma.
PB  - Croatian Association for Cancer Research, Zagreb, Croatia
C3  - “HDIR-6: Targeting Cancer”, The 6th Meeting of the Croatian Association for Cancer Research with International Participation, November 10-12, 2022, Book of Abstracts
T1  - Antimelanoma Potential of New Telmisartan Analogues Without AT1 Receptor Activity
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5450
ER  - 

@conference{
author = "Damjanović, Ana and Vuković, Miodrag and Vukadinović, Dragana and Dobričić, Vladimir and Grahovac, Jelena",
year = "2022",
abstract = "Melanoma is one of the most aggressive malignancies, where the prognosis for metastatic patients remains extremely poor. Our group has shown that the antihypertensive drug telmisartan has antimelanoma potential1. Given that the antihypertensive effect is not favorable in cancer patients, the aim of this study was to design and test novel telmisartan derivatives without the angiotensin receptor 1 (AT1R) binding activity. New derivatives were designed by modification of the carboxylic group, in order to alter telmisartan geometry and its AT1R binding properties. Eight derivatives, from which the lack of AT1R antagonistic activity could be expected based on molecular docking, were synthetized and selected for in vitro testing. After the cytotoxicity test on human melanoma cell lines A375 and 518a2, three derivatives that were twice more potent than telmisartan itself were selected for further analysis. The new derivatives induced mitochondrial fragmentation, generation of the mitochondrial reactive oxygen species, and decrease of mitochondrial membrane potential in melanoma cells, the mechanism we previously shown for induction of apoptosis by telmisartan in melanoma cells. As the new derivatives showed more potent effect on melanoma cells than telmisartan these results lay a ground for further preclinical testing in melanoma.",
publisher = "Croatian Association for Cancer Research, Zagreb, Croatia",
journal = "“HDIR-6: Targeting Cancer”, The 6th Meeting of the Croatian Association for Cancer Research with International Participation, November 10-12, 2022, Book of Abstracts",
title = "Antimelanoma Potential of New Telmisartan Analogues Without AT1 Receptor Activity",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5450"
}

Damjanović, A., Vuković, M., Vukadinović, D., Dobričić, V.,& Grahovac, J.. (2022). Antimelanoma Potential of New Telmisartan Analogues Without AT1 Receptor Activity. in “HDIR-6: Targeting Cancer”, The 6th Meeting of the Croatian Association for Cancer Research with International Participation, November 10-12, 2022, Book of Abstracts
Croatian Association for Cancer Research, Zagreb, Croatia., 20-20.
https://hdl.handle.net/21.15107/rcub_farfar_5450

Damjanović A, Vuković M, Vukadinović D, Dobričić V, Grahovac J. Antimelanoma Potential of New Telmisartan Analogues Without AT1 Receptor Activity. in “HDIR-6: Targeting Cancer”, The 6th Meeting of the Croatian Association for Cancer Research with International Participation, November 10-12, 2022, Book of Abstracts. 2022;:20-20.
https://hdl.handle.net/21.15107/rcub_farfar_5450 .

Damjanović, Ana, Vuković, Miodrag, Vukadinović, Dragana, Dobričić, Vladimir, Grahovac, Jelena, "Antimelanoma Potential of New Telmisartan Analogues Without AT1 Receptor Activity" in “HDIR-6: Targeting Cancer”, The 6th Meeting of the Croatian Association for Cancer Research with International Participation, November 10-12, 2022, Book of Abstracts (2022):20-20,
https://hdl.handle.net/21.15107/rcub_farfar_5450 .

TY  - JOUR
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Daković, Aleksandra
AU  - Damjanović, Ana
AU  - Krstić, Jugoslav
AU  - Stojanović, Jovica
AU  - Čalija, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3796
AB  - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
VL  - 123
DO  - 10.1016/j.msec.2021.112029
ER  - 

@article{
author = "Jauković, Valentina and Krajišnik, Danina and Daković, Aleksandra and Damjanović, Ana and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan",
year = "2021",
abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release",
volume = "123",
doi = "10.1016/j.msec.2021.112029"
}

Jauković, V., Krajišnik, D., Daković, A., Damjanović, A., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C
Elsevier Ltd., 123.
https://doi.org/10.1016/j.msec.2021.112029

Jauković V, Krajišnik D, Daković A, Damjanović A, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123.
doi:10.1016/j.msec.2021.112029 .

Jauković, Valentina, Krajišnik, Danina, Daković, Aleksandra, Damjanović, Ana, Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021),
https://doi.org/10.1016/j.msec.2021.112029 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Mitsou, Evgenia
AU  - Damjanović, Ana
AU  - Papadimitriou, Vassiliki
AU  - Antić-Stanković, Jelena
AU  - Stanojević, Boban
AU  - Xenakis, Aristotelis
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3528
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI < 0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity
VL  - 301
DO  - 10.1016/j.molliq.2020.112479
ER  - 

@article{
author = "Nikolić, Ines and Mitsou, Evgenia and Damjanović, Ana and Papadimitriou, Vassiliki and Antić-Stanković, Jelena and Stanojević, Boban and Xenakis, Aristotelis and Savić, Snežana",
year = "2020",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI < 0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity",
volume = "301",
doi = "10.1016/j.molliq.2020.112479"
}

Nikolić, I., Mitsou, E., Damjanović, A., Papadimitriou, V., Antić-Stanković, J., Stanojević, B., Xenakis, A.,& Savić, S.. (2020). Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity. in Journal of Molecular Liquids
Elsevier B.V.., 301.
https://doi.org/10.1016/j.molliq.2020.112479

Nikolić I, Mitsou E, Damjanović A, Papadimitriou V, Antić-Stanković J, Stanojević B, Xenakis A, Savić S. Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity. in Journal of Molecular Liquids. 2020;301.
doi:10.1016/j.molliq.2020.112479 .

Nikolić, Ines, Mitsou, Evgenia, Damjanović, Ana, Papadimitriou, Vassiliki, Antić-Stanković, Jelena, Stanojević, Boban, Xenakis, Aristotelis, Savić, Snežana, "Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity" in Journal of Molecular Liquids, 301 (2020),
https://doi.org/10.1016/j.molliq.2020.112479 . .

TY  - JOUR
AU  - Damjanović, Ana
AU  - Kolundžija, Branka
AU  - Matić, Ivana
AU  - Krivokuća, Ana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Janković, Radmila
AU  - Antić-Stanković, Jelena
AU  - Stanojković, Tatjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3738
AB  - Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.
PB  - MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro
VL  - 25
IS  - 22
DO  - 10.3390/molecules25225233
ER  - 

@article{
author = "Damjanović, Ana and Kolundžija, Branka and Matić, Ivana and Krivokuća, Ana and Zdunić, Gordana and Šavikin, Katarina and Janković, Radmila and Antić-Stanković, Jelena and Stanojković, Tatjana",
year = "2020",
abstract = "Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.",
publisher = "MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro",
volume = "25",
number = "22",
doi = "10.3390/molecules25225233"
}

Damjanović, A., Kolundžija, B., Matić, I., Krivokuća, A., Zdunić, G., Šavikin, K., Janković, R., Antić-Stanković, J.,& Stanojković, T.. (2020). Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland)
MDPI., 25(22).
https://doi.org/10.3390/molecules25225233

Damjanović A, Kolundžija B, Matić I, Krivokuća A, Zdunić G, Šavikin K, Janković R, Antić-Stanković J, Stanojković T. Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland). 2020;25(22).
doi:10.3390/molecules25225233 .

Damjanović, Ana, Kolundžija, Branka, Matić, Ivana, Krivokuća, Ana, Zdunić, Gordana, Šavikin, Katarina, Janković, Radmila, Antić-Stanković, Jelena, Stanojković, Tatjana, "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro" in Molecules (Basel, Switzerland), 25, no. 22 (2020),
https://doi.org/10.3390/molecules25225233 . .

TY  - JOUR
AU  - Cvetanović, Aleksandra
AU  - Zekovic, Zoran
AU  - Svarc-Gajic, Jaroslava
AU  - Ražić, Slavica
AU  - Damjanović, Ana B.
AU  - Zengin, Gokhan
AU  - Delerue-Matos, Cristina
AU  - Moreira, Manuela
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3194
AB  - BACKGROUND: To obtain bioactive fractions from plant material subcritical water is notably advantageous in comparison to any other technique. Excellent solvating properties and selectivity combined with finely tuned reactivity of subcritical water enable exploitation of the potential of plants. Herein subcritical water extraction was used for recovery of bioactive compounds from leaves, roots and fruits of Sambucus ebulus L. Extracts obtained were characterized in terms of biological and chemical fingerprints. RESULTS AND DISCUSSIONR: esults obtained by using several antioxidant assays that focused on different mechanisms showed that subcritical water extracts of Sambucus ebulus were powerful antioxidants. Enzyme inhibitory effects were tested against alpha-amylase, alpha-glucosidase and tyrosinase, and the results showed the anti-diabetic potential of the extracts as well as its possible use with skin disorders. Antiproliferative properties were detected on three cell lines (A-549; LS-174 T; HeLa) and showed prominent cytotoxicity against all tumor cell lines. Inhibitory concentrations obtained were in the range 0.58-8.10 mu g mL(-1). Generally, the SCW extracts from leaves exhibited stronger biological activities with higher levels of phenolic compounds compared with the roots and fruits of Sambucus ebulus. Gallic acid, catechin and caffeic acid were identified as major components in these extracts and these components seem to relate with observed biological activity. CONCLUSION: The results obtained suggest that Sambucus ebulus has great potential for preparing new phyto-pharmaceuticals and functional food ingredients. (C) 2017 Society of Chemical Industry.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.
VL  - 93
IS  - 4
SP  - 1097
EP  - 1104
DO  - 10.1002/jctb.5468
ER  - 

@article{
author = "Cvetanović, Aleksandra and Zekovic, Zoran and Svarc-Gajic, Jaroslava and Ražić, Slavica and Damjanović, Ana B. and Zengin, Gokhan and Delerue-Matos, Cristina and Moreira, Manuela",
year = "2018",
abstract = "BACKGROUND: To obtain bioactive fractions from plant material subcritical water is notably advantageous in comparison to any other technique. Excellent solvating properties and selectivity combined with finely tuned reactivity of subcritical water enable exploitation of the potential of plants. Herein subcritical water extraction was used for recovery of bioactive compounds from leaves, roots and fruits of Sambucus ebulus L. Extracts obtained were characterized in terms of biological and chemical fingerprints. RESULTS AND DISCUSSIONR: esults obtained by using several antioxidant assays that focused on different mechanisms showed that subcritical water extracts of Sambucus ebulus were powerful antioxidants. Enzyme inhibitory effects were tested against alpha-amylase, alpha-glucosidase and tyrosinase, and the results showed the anti-diabetic potential of the extracts as well as its possible use with skin disorders. Antiproliferative properties were detected on three cell lines (A-549; LS-174 T; HeLa) and showed prominent cytotoxicity against all tumor cell lines. Inhibitory concentrations obtained were in the range 0.58-8.10 mu g mL(-1). Generally, the SCW extracts from leaves exhibited stronger biological activities with higher levels of phenolic compounds compared with the roots and fruits of Sambucus ebulus. Gallic acid, catechin and caffeic acid were identified as major components in these extracts and these components seem to relate with observed biological activity. CONCLUSION: The results obtained suggest that Sambucus ebulus has great potential for preparing new phyto-pharmaceuticals and functional food ingredients. (C) 2017 Society of Chemical Industry.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.",
volume = "93",
number = "4",
pages = "1097-1104",
doi = "10.1002/jctb.5468"
}

Cvetanović, A., Zekovic, Z., Svarc-Gajic, J., Ražić, S., Damjanović, A. B., Zengin, G., Delerue-Matos, C.,& Moreira, M.. (2018). A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 93(4), 1097-1104.
https://doi.org/10.1002/jctb.5468

Cvetanović A, Zekovic Z, Svarc-Gajic J, Ražić S, Damjanović AB, Zengin G, Delerue-Matos C, Moreira M. A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.. in Journal of Chemical Technology and Biotechnology. 2018;93(4):1097-1104.
doi:10.1002/jctb.5468 .

Cvetanović, Aleksandra, Zekovic, Zoran, Svarc-Gajic, Jaroslava, Ražić, Slavica, Damjanović, Ana B., Zengin, Gokhan, Delerue-Matos, Cristina, Moreira, Manuela, "A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L." in Journal of Chemical Technology and Biotechnology, 93, no. 4 (2018):1097-1104,
https://doi.org/10.1002/jctb.5468 . .

TY  - JOUR
AU  - Cvetanović, Aleksandra
AU  - Zengin, Gokhan
AU  - Zekovic, Zoran
AU  - Svarc-Gajic, Jaroslava
AU  - Ražić, Slavica
AU  - Damjanović, Ana B.
AU  - Magkovic, Pavle
AU  - Mitic, Milan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3056
AB  - Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction
VL  - 121
SP  - 458
EP  - 466
DO  - 10.1016/j.fct.2018.09.045
ER  - 

@article{
author = "Cvetanović, Aleksandra and Zengin, Gokhan and Zekovic, Zoran and Svarc-Gajic, Jaroslava and Ražić, Slavica and Damjanović, Ana B. and Magkovic, Pavle and Mitic, Milan",
year = "2018",
abstract = "Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction",
volume = "121",
pages = "458-466",
doi = "10.1016/j.fct.2018.09.045"
}

Cvetanović, A., Zengin, G., Zekovic, Z., Svarc-Gajic, J., Ražić, S., Damjanović, A. B., Magkovic, P.,& Mitic, M.. (2018). Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 121, 458-466.
https://doi.org/10.1016/j.fct.2018.09.045

Cvetanović A, Zengin G, Zekovic Z, Svarc-Gajic J, Ražić S, Damjanović AB, Magkovic P, Mitic M. Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology. 2018;121:458-466.
doi:10.1016/j.fct.2018.09.045 .

Cvetanović, Aleksandra, Zengin, Gokhan, Zekovic, Zoran, Svarc-Gajic, Jaroslava, Ražić, Slavica, Damjanović, Ana B., Magkovic, Pavle, Mitic, Milan, "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction" in Food and Chemical Toxicology, 121 (2018):458-466,
https://doi.org/10.1016/j.fct.2018.09.045 . .

TY  - THES
AU  - Damjanović, Ana B.
PY  - 2018
UR  - http://nardus.mpn.gov.rs/handle/123456789/9479
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5790
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:17663/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50063119
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3674
AB  - Na osnovu podataka o upotrebi vrste Mahonia aquifolium u tradicionalnoj medicini definisan je cilj našeg istraživanja, ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata kore mahonije.Ispitivanjem citotoksičnosti vodenog i etanolnog ekstrakta na humanim malignim ćelijskim linijama je dokazano da ekstrakti imaju selektivnu citotoksičnu aktivnost, a najviši intenzitet dejstva su pokazali na HeLa ćelijama adenokarcinoma cerviksa. Fluorescentnom mikroskopijom i analizom faza ćelijskog ciklusa je pokazano da ekstrakti indukuju apoptozu u HeLa ćelijama. Primenom inhibitora kaspaza pokazano je da ekstrakti indukuju apoptozu preko spoljašnjeg puta aktivacije. Ispitivanja na endotelijalnim EA.hy926 ćelijama pokazala su antiangiogenetski i antimigratorni efekat ekstrakata.Primena tretmana doksorubicin/ekstrakt ukazala je na izraženo sinergističko dejstvo ekstrakta sa doksorubicinom na A549 ćelijama karcinoma pluća. Ekstrakti povećavaju zadržavanje leka u ćeliji i pojačavaju efekat doksorubicina na smanjenje migracije A549 ćelija i njihovo grupisanje u kolonije. Antimetastatski potencijal kombinacije doksorubicin/ekstrakt potvrđen je analizom ekspresije gena odgovornih za invazivnost.Oba ekstrakta ispoljavaju antimikrobni efekat, koji je najizraženiji na bakterijskoj vrsti Staphylococcus aureus. Ekstrakti dovode do smanjenja produkcije biofilma i glikokaliksa. Sa antibioticima i antimikoticima ekstrakti su delovali sinergistički.Hemijskom analizom potvrđeno je da su za aktivnost ekstrakata M. aquifolium odgovorni alkaloidi tipa berberina. Na intenzitet citotoksičnosti najviše utiču berberin, palmatin i berbamin.Rezultati su pokazali da M. aquifolium može naći svoje mesto u terapiji malignih ili infektivnih oboljenja, pojedinačno i u kombinaciji sa postojećim lekovima.
AB  - The goal of our research was investigation of antitumor and antimicrobial activities of Mahonia aquifolium extracts and it was based on its use in traditional medicine. Water and ethanol extract exerted selective cytotoxic activity on examined malignant cell lines, and the best activity was observed against cervical adenocarcinoma, HeLa, cells. Fluorescence microscopy and cell cycle analysis showed that both extracts induce apoptosis in HeLa cells. Experiments with caspase inhibitors showed that extracts induce a caspase-dependent apoptosis through extrinsic pathway. Both extracts inhibit the migration of EA.hy926 cells, and exerted anti-angiogenic effect.The investigation of the doxorubicine/extract treatment showed synergistic effect of extracts with doxorubicine against lung carcinoma A549, cells. Extracts were shown to prolong doxorubicin retention time in A549 cells. A549 cells treated with doxorubicin/extract were shown to have lower migratory and colony formation potentials than the cells treated with doxorubicin alone. Antimetastatic effect of the treatment was confirmed with analysis of expression of genes responsible for malignant cells invasiveness.Extracts exerted antimicrobial effect. The best effect was observed against Staphylococcus aureus. Extracts showed potential to inhibit production of biofilm and glycocalycs. The use of extracts with antibiotics and antimycotics showed synergistic effect.Chemical analysis confirmed that berberine type alkaloids are the most responsible for activities of M. aquifolium. The compounds with the highest cytotoxic activity are: berberine, palmatine, and berbamine.The results of our research suggested that M. aquifolium may be used in therapy of malignant or infective diseases, alone or in combination with other drugs.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9479
ER  - 

@phdthesis{
author = "Damjanović, Ana B.",
year = "2018",
abstract = "Na osnovu podataka o upotrebi vrste Mahonia aquifolium u tradicionalnoj medicini definisan je cilj našeg istraživanja, ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata kore mahonije.Ispitivanjem citotoksičnosti vodenog i etanolnog ekstrakta na humanim malignim ćelijskim linijama je dokazano da ekstrakti imaju selektivnu citotoksičnu aktivnost, a najviši intenzitet dejstva su pokazali na HeLa ćelijama adenokarcinoma cerviksa. Fluorescentnom mikroskopijom i analizom faza ćelijskog ciklusa je pokazano da ekstrakti indukuju apoptozu u HeLa ćelijama. Primenom inhibitora kaspaza pokazano je da ekstrakti indukuju apoptozu preko spoljašnjeg puta aktivacije. Ispitivanja na endotelijalnim EA.hy926 ćelijama pokazala su antiangiogenetski i antimigratorni efekat ekstrakata.Primena tretmana doksorubicin/ekstrakt ukazala je na izraženo sinergističko dejstvo ekstrakta sa doksorubicinom na A549 ćelijama karcinoma pluća. Ekstrakti povećavaju zadržavanje leka u ćeliji i pojačavaju efekat doksorubicina na smanjenje migracije A549 ćelija i njihovo grupisanje u kolonije. Antimetastatski potencijal kombinacije doksorubicin/ekstrakt potvrđen je analizom ekspresije gena odgovornih za invazivnost.Oba ekstrakta ispoljavaju antimikrobni efekat, koji je najizraženiji na bakterijskoj vrsti Staphylococcus aureus. Ekstrakti dovode do smanjenja produkcije biofilma i glikokaliksa. Sa antibioticima i antimikoticima ekstrakti su delovali sinergistički.Hemijskom analizom potvrđeno je da su za aktivnost ekstrakata M. aquifolium odgovorni alkaloidi tipa berberina. Na intenzitet citotoksičnosti najviše utiču berberin, palmatin i berbamin.Rezultati su pokazali da M. aquifolium može naći svoje mesto u terapiji malignih ili infektivnih oboljenja, pojedinačno i u kombinaciji sa postojećim lekovima., The goal of our research was investigation of antitumor and antimicrobial activities of Mahonia aquifolium extracts and it was based on its use in traditional medicine. Water and ethanol extract exerted selective cytotoxic activity on examined malignant cell lines, and the best activity was observed against cervical adenocarcinoma, HeLa, cells. Fluorescence microscopy and cell cycle analysis showed that both extracts induce apoptosis in HeLa cells. Experiments with caspase inhibitors showed that extracts induce a caspase-dependent apoptosis through extrinsic pathway. Both extracts inhibit the migration of EA.hy926 cells, and exerted anti-angiogenic effect.The investigation of the doxorubicine/extract treatment showed synergistic effect of extracts with doxorubicine against lung carcinoma A549, cells. Extracts were shown to prolong doxorubicin retention time in A549 cells. A549 cells treated with doxorubicin/extract were shown to have lower migratory and colony formation potentials than the cells treated with doxorubicin alone. Antimetastatic effect of the treatment was confirmed with analysis of expression of genes responsible for malignant cells invasiveness.Extracts exerted antimicrobial effect. The best effect was observed against Staphylococcus aureus. Extracts showed potential to inhibit production of biofilm and glycocalycs. The use of extracts with antibiotics and antimycotics showed synergistic effect.Chemical analysis confirmed that berberine type alkaloids are the most responsible for activities of M. aquifolium. The compounds with the highest cytotoxic activity are: berberine, palmatine, and berbamine.The results of our research suggested that M. aquifolium may be used in therapy of malignant or infective diseases, alone or in combination with other drugs.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9479"
}

Damjanović, A. B.. (2018). In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9479

Damjanović AB. In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_9479 .

Damjanović, Ana B., "In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt." in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_9479 .

TY  - JOUR
AU  - Simić, Milena
AU  - Damjanović, Ana B.
AU  - Kalinić, Marko
AU  - Tasić, Gordana
AU  - Erić, Slavica
AU  - Antić-Stanković, Jelena
AU  - Savić, Vladimir
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2606
AB  - A novel and efficient synthetic route was developed for the preparation of protoberberine derivatives. A methodology, designed primarily to control the substitution patterns on the terminal rings, was used to access a small array of these compounds. An initial biological profiling suggested an anticancer potential of the synthesised derivatives, while structure-based target fishing identified their potential targets and established a rational basis for further structural modifications. Although the activities need further improvement, the study demonstrated that the described approach may be useful in the discovery of novel lead compounds.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis, cytotoxicity and computational study of novel protoberberine derivatives
VL  - 81
IS  - 2
SP  - 103
EP  - 123
DO  - 10.2298/JSC150525090S
ER  - 

@article{
author = "Simić, Milena and Damjanović, Ana B. and Kalinić, Marko and Tasić, Gordana and Erić, Slavica and Antić-Stanković, Jelena and Savić, Vladimir",
year = "2016",
abstract = "A novel and efficient synthetic route was developed for the preparation of protoberberine derivatives. A methodology, designed primarily to control the substitution patterns on the terminal rings, was used to access a small array of these compounds. An initial biological profiling suggested an anticancer potential of the synthesised derivatives, while structure-based target fishing identified their potential targets and established a rational basis for further structural modifications. Although the activities need further improvement, the study demonstrated that the described approach may be useful in the discovery of novel lead compounds.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis, cytotoxicity and computational study of novel protoberberine derivatives",
volume = "81",
number = "2",
pages = "103-123",
doi = "10.2298/JSC150525090S"
}

Simić, M., Damjanović, A. B., Kalinić, M., Tasić, G., Erić, S., Antić-Stanković, J.,& Savić, V.. (2016). Synthesis, cytotoxicity and computational study of novel protoberberine derivatives. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 81(2), 103-123.
https://doi.org/10.2298/JSC150525090S

Simić M, Damjanović AB, Kalinić M, Tasić G, Erić S, Antić-Stanković J, Savić V. Synthesis, cytotoxicity and computational study of novel protoberberine derivatives. in Journal of the Serbian Chemical Society. 2016;81(2):103-123.
doi:10.2298/JSC150525090S .

Simić, Milena, Damjanović, Ana B., Kalinić, Marko, Tasić, Gordana, Erić, Slavica, Antić-Stanković, Jelena, Savić, Vladimir, "Synthesis, cytotoxicity and computational study of novel protoberberine derivatives" in Journal of the Serbian Chemical Society, 81, no. 2 (2016):103-123,
https://doi.org/10.2298/JSC150525090S . .

TY  - JOUR
AU  - Damjanović, Ana B.
AU  - Matić, Ivana Z.
AU  - Dordić, Marija
AU  - Nikolić-Durović, Marina
AU  - Nikolić, Srdan
AU  - Roki, Ksenija
AU  - Milovanović, Zorka
AU  - Antić-Stanković, Jelena
AU  - Dzodić, Radan
AU  - Damjanović, Svetozar S.
AU  - Kanjer, Ksenija
AU  - Abu Rabi, Zaki
AU  - Juranić, Zorica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2457
AB  - The aim of research was to determine the effects of maximally therapeutically achievable concentrations of metformin on malignant cells and healthy peripheral blood mononuclear cells (PBMC). Eight patients with T2D or hyperglycemia and nine healthy volunteers were included in the study. For determination of the influence of metformin on the phenotype of breast carcinoma, 1,410 patients with surgically removed tumors were included. From this group 37 breast cancer patients had DM type 2 or hyperglycemia and were pretreated with metformin alone or sometimes in combination with other antidiabetic drugs. Our results proved that metformin at low concentrations induced mild decrease in survival of malignant cells and PBMC stimulated for proliferation, but it didn't affect survival of resting PBMC. The effects of plasma of hyperglycemic patients who were under metformin therapy on autologous PBMC-induced decrease in survival of MDA-MB-361 cells, was noticeable in some patients. Metformin pretreatment for 24 h of HER2+ MDA-MB-361 cells, which were subsequently treated for 48 h with Herceptin, induced additional decline in cell survival. The analysis of influence of metformin on phenotype of breast cancer cells revealed significantly lower number of diabetic cancer patients treated with metformin with overexpressed HER2+ tumors (p  lt  0.013), while the number of patients with ER+PR+ tumors was not significantly changed (p  lt  0.832). In conclusion, therapeutically used concentrations of metformin exhibit mild cytotoxic action on malignant and dividing normal cells pointing to its preferred role in malignant and autoimmune diseases. The use of metformin was associated with pronounced decrease in HER2 overexpressing tumors.
PB  - Springer, Dordrecht
T2  - Pathology & Oncology Research
T1  - Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells
VL  - 21
IS  - 3
SP  - 605
EP  - 612
DO  - 10.1007/s12253-014-9864-9
ER  - 

@article{
author = "Damjanović, Ana B. and Matić, Ivana Z. and Dordić, Marija and Nikolić-Durović, Marina and Nikolić, Srdan and Roki, Ksenija and Milovanović, Zorka and Antić-Stanković, Jelena and Dzodić, Radan and Damjanović, Svetozar S. and Kanjer, Ksenija and Abu Rabi, Zaki and Juranić, Zorica",
year = "2015",
abstract = "The aim of research was to determine the effects of maximally therapeutically achievable concentrations of metformin on malignant cells and healthy peripheral blood mononuclear cells (PBMC). Eight patients with T2D or hyperglycemia and nine healthy volunteers were included in the study. For determination of the influence of metformin on the phenotype of breast carcinoma, 1,410 patients with surgically removed tumors were included. From this group 37 breast cancer patients had DM type 2 or hyperglycemia and were pretreated with metformin alone or sometimes in combination with other antidiabetic drugs. Our results proved that metformin at low concentrations induced mild decrease in survival of malignant cells and PBMC stimulated for proliferation, but it didn't affect survival of resting PBMC. The effects of plasma of hyperglycemic patients who were under metformin therapy on autologous PBMC-induced decrease in survival of MDA-MB-361 cells, was noticeable in some patients. Metformin pretreatment for 24 h of HER2+ MDA-MB-361 cells, which were subsequently treated for 48 h with Herceptin, induced additional decline in cell survival. The analysis of influence of metformin on phenotype of breast cancer cells revealed significantly lower number of diabetic cancer patients treated with metformin with overexpressed HER2+ tumors (p  lt  0.013), while the number of patients with ER+PR+ tumors was not significantly changed (p  lt  0.832). In conclusion, therapeutically used concentrations of metformin exhibit mild cytotoxic action on malignant and dividing normal cells pointing to its preferred role in malignant and autoimmune diseases. The use of metformin was associated with pronounced decrease in HER2 overexpressing tumors.",
publisher = "Springer, Dordrecht",
journal = "Pathology & Oncology Research",
title = "Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells",
volume = "21",
number = "3",
pages = "605-612",
doi = "10.1007/s12253-014-9864-9"
}

Damjanović, A. B., Matić, I. Z., Dordić, M., Nikolić-Durović, M., Nikolić, S., Roki, K., Milovanović, Z., Antić-Stanković, J., Dzodić, R., Damjanović, S. S., Kanjer, K., Abu Rabi, Z.,& Juranić, Z.. (2015). Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells. in Pathology & Oncology Research
Springer, Dordrecht., 21(3), 605-612.
https://doi.org/10.1007/s12253-014-9864-9

Damjanović AB, Matić IZ, Dordić M, Nikolić-Durović M, Nikolić S, Roki K, Milovanović Z, Antić-Stanković J, Dzodić R, Damjanović SS, Kanjer K, Abu Rabi Z, Juranić Z. Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells. in Pathology & Oncology Research. 2015;21(3):605-612.
doi:10.1007/s12253-014-9864-9 .

Damjanović, Ana B., Matić, Ivana Z., Dordić, Marija, Nikolić-Durović, Marina, Nikolić, Srdan, Roki, Ksenija, Milovanović, Zorka, Antić-Stanković, Jelena, Dzodić, Radan, Damjanović, Svetozar S., Kanjer, Ksenija, Abu Rabi, Zaki, Juranić, Zorica, "Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells" in Pathology & Oncology Research, 21, no. 3 (2015):605-612,
https://doi.org/10.1007/s12253-014-9864-9 . .