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Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma
Razvoj i validacija spe-HPLC metode za određivanje karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u plazmi
dc.creator | Džodić, Predrag | |
dc.creator | Živanović, Ljiljana | |
dc.creator | Protić, Ana | |
dc.creator | Ivanović, Ivana | |
dc.creator | Veličković-Radovanović, Radmila | |
dc.creator | Spasić, Mirjana | |
dc.creator | Lukić, Stevo | |
dc.creator | Živanović, Slavoljub | |
dc.date.accessioned | 2019-09-02T11:31:57Z | |
dc.date.available | 2019-09-02T11:31:57Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1823 | |
dc.description.abstract | A solid phase extraction-HPLC method has been developed and validated for the rapid analysis of carbamazepine and its two metabolites, carbamazepine epoxide and carbamazepine trans-diol, in human plasma. The analysis was performed using a C18 Bakerbond-BDC analytical column (250 mm×4.6 mm i.d., particle size 5 μm). The optimal conditions for the separation were established with the mobile phase acetonitrile - 10 mM phosphate buffer, pH 7.0 (30:70, v/v) at a flow rate of 1.5 mL min-1 and temperature of 35°C, with UV detection at 210 nm. The total run time was about 8 minutes. The SPE procedure for the extraction of the analytes from a plasma sample was developed using Oasis HLB cartridges and subsequently, the eluate was injected into the HPLC system for analysis. Afterwards, the SPE-HPLC method was subjected to validation. Linearity was obtained over the concentration range of 0.2-25 μg mL-1 for carbamazepine, carbamazepine epoxide and carbamazepine trans-diol, with correlation coefficients higher than 0.995. The method showed good intra-day and inter-day precision with a relative standard deviation below 7.96 %, while the accuracy ranged from 92.09 to 108.5 % for all analytes. Finally, the method was successfully applied to the analysis of the plasma samples of epileptic patients in mono- and polytherapy. | en |
dc.description.abstract | SPE-HPLC metoda je razvijena i validirana u cilju brzog analiziranja karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u humanoj plazmi. C18 Bakerbond-BDC analitička kolona (250 mm x 4,6 mm; 5 μm) je korišćena radi izvođenja analize. Optimalni uslovi za hromatografsko razdvajanje su mobilna faza acetonitril - 10 mM fosfatni pufer, pH 7,0 (30:70, v/v), protok od 1,5 ml min-1, temperatura 35°C i detekcija na 210 nm. Ukupno trajanje hromatografskog rana iznosi oko 8 min. SPE procedura za ekstrakciju analita iz uzoraka plazme je razvijena uz korišćenje Oasis HLB ketridža nakon čega se eluat injektuje u HPLC sistem radi analiziranja. Zatim je izvršena validacija SPE-HPLC metode. Linearnost je potvrđena u koncentracionom opsegu 0,2-25 μg/ml za karbamazepin, karbamazepin epoksid i karbamazepin trans-diol sa vrednošću korelacionih koeficijenata višom od 0,995. Preciznost metode u toku jednog i u toku više dana je dobra sa relativnom standardnom devijacijom nižom od 7,96 %, dok tačnost metode obuhvata vrednosti u opsegu od 92,09 do 108,5 % za sve analite. Na kraju je metoda uspešno primenjena u cilju analiziranja uzoraka plazme pacijenata obolelih od epilepsije na monoterapiji i politerapiji. | sr |
dc.publisher | Srpsko hemijsko društvo, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | human plasma | en |
dc.subject | carbamazepine | en |
dc.subject | carbamazepine epoxide | en |
dc.subject | carbamazepine trans-diol | en |
dc.subject | solid phase extraction | en |
dc.title | Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma | en |
dc.title | Razvoj i validacija spe-HPLC metode za određivanje karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u plazmi | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Живановић, Љиљана; Джодић, Предраг; Живановић, Славољуб; Ивановић, Ивана; Величковић-Радовановић, Радмила; Протић, Aна; Спасић, Мирјана; Лукић, Стево; Развој и валидација спе-ХПЛЦ методе за одређивање карбамазепина и метаболита карбамазепин епоксида и карбамазепин транс-диола у плазми; Развој и валидација спе-ХПЛЦ методе за одређивање карбамазепина и метаболита карбамазепин епоксида и карбамазепин транс-диола у плазми; | |
dc.citation.volume | 77 | |
dc.citation.issue | 10 | |
dc.citation.spage | 1423 | |
dc.citation.epage | 1436 | |
dc.citation.other | 77(10): 1423-1436 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000311594100010 | |
dc.identifier.doi | 10.2298/JSC120106084D | |
dc.identifier.scopus | 2-s2.0-84870184029 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs//bitstream/id/623/1821.pdf | |
dc.type.version | publishedVersion |