Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population
Само за регистроване кориснике
2006
Аутори
Kotur-Stevuljević, JelenaSpasić, Slavica
Stefanović, Aleksandra
Zeljković, Aleksandra
Bogavac-Stanojević, Nataša
Kalimanovska-Oštrić, Dimitra
Spasojević-Kalimanovska, Vesna
Jelić-Ivanović, Zorana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Paraoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated serum enzyme that protects lipoproteins from oxidative modifications. Polymorphisms in the gene, including PON1(Q192R), have been studied. However, inconsistencies regarding the above-mentioned polymorphism obscure its association with vascular disease. Methods: Using a two-substrate (paraoxon/diazoxon) activity method, we investigated the frequencies of PON1Q192R phenotypes in 261 middle-aged subjects: 156 patients with angiographically assessed coronary heart disease (CHD) and 105 CHD-free subjects as the control group. The PON1(192) phenotype was predicted from examination of the two-dimensional plot of hydrolysis rates of diazoxon vs. paraoxon and by using the antimode of the histogram of the ratio of diazoxonase/paraoxonase activity. Results: The PON1Q192R phenotype frequencies in 113 patients with occlusion) 50% (coronary artery disease-positive, CAD+ group) vs. control population were as follows: QQ ...(0.552 vs. 0.510), QR (0.382 vs. 0.408) and RR (0.066 vs. 0.082); chi(2) = 0.414, p = 0.813. We found lower paraoxonase (POase) and diazoxonase (DZOase) activities in the CAD+ patients when compared to the control population. According to logistic regression analysis, POase activity was a better predictor of coronary disease onset compared with DZOase activity measurements and PON1Q192R phenotyping. Conclusions: We conclude that enzyme activity (within a particular phenotypic group) is more important than phenotype alone in predicting susceptibility to coronary artery disease.
Кључне речи:
coronary artery disease / paraoxonase-1 (PON1) activity / PON1(Q192R) phenotypeИзвор:
Clinical Chemistry and Laboratory Medicine, 2006, 44, 10, 1206-1213Издавач:
- Walter de Gruyter Gmbh, Berlin
Финансирање / пројекти:
- Испитивање биохемијских и генетичких фактора ризика као узрочника и маркера атеросклерозе и других обољења: аналитички и клинички аспекти (RS-MESTD-MPN2006-2010-145036)
DOI: 10.1515/CCLM.2006.216
ISSN: 1434-6621
PubMed: 17032132
WoS: 000241193900007
Scopus: 2-s2.0-33750072918
Институција/група
PharmacyTY - JOUR AU - Kotur-Stevuljević, Jelena AU - Spasić, Slavica AU - Stefanović, Aleksandra AU - Zeljković, Aleksandra AU - Bogavac-Stanojević, Nataša AU - Kalimanovska-Oštrić, Dimitra AU - Spasojević-Kalimanovska, Vesna AU - Jelić-Ivanović, Zorana PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/697 AB - Background: Paraoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated serum enzyme that protects lipoproteins from oxidative modifications. Polymorphisms in the gene, including PON1(Q192R), have been studied. However, inconsistencies regarding the above-mentioned polymorphism obscure its association with vascular disease. Methods: Using a two-substrate (paraoxon/diazoxon) activity method, we investigated the frequencies of PON1Q192R phenotypes in 261 middle-aged subjects: 156 patients with angiographically assessed coronary heart disease (CHD) and 105 CHD-free subjects as the control group. The PON1(192) phenotype was predicted from examination of the two-dimensional plot of hydrolysis rates of diazoxon vs. paraoxon and by using the antimode of the histogram of the ratio of diazoxonase/paraoxonase activity. Results: The PON1Q192R phenotype frequencies in 113 patients with occlusion) 50% (coronary artery disease-positive, CAD+ group) vs. control population were as follows: QQ (0.552 vs. 0.510), QR (0.382 vs. 0.408) and RR (0.066 vs. 0.082); chi(2) = 0.414, p = 0.813. We found lower paraoxonase (POase) and diazoxonase (DZOase) activities in the CAD+ patients when compared to the control population. According to logistic regression analysis, POase activity was a better predictor of coronary disease onset compared with DZOase activity measurements and PON1Q192R phenotyping. Conclusions: We conclude that enzyme activity (within a particular phenotypic group) is more important than phenotype alone in predicting susceptibility to coronary artery disease. PB - Walter de Gruyter Gmbh, Berlin T2 - Clinical Chemistry and Laboratory Medicine T1 - Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population VL - 44 IS - 10 SP - 1206 EP - 1213 DO - 10.1515/CCLM.2006.216 ER -
@article{ author = "Kotur-Stevuljević, Jelena and Spasić, Slavica and Stefanović, Aleksandra and Zeljković, Aleksandra and Bogavac-Stanojević, Nataša and Kalimanovska-Oštrić, Dimitra and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana", year = "2006", abstract = "Background: Paraoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated serum enzyme that protects lipoproteins from oxidative modifications. Polymorphisms in the gene, including PON1(Q192R), have been studied. However, inconsistencies regarding the above-mentioned polymorphism obscure its association with vascular disease. Methods: Using a two-substrate (paraoxon/diazoxon) activity method, we investigated the frequencies of PON1Q192R phenotypes in 261 middle-aged subjects: 156 patients with angiographically assessed coronary heart disease (CHD) and 105 CHD-free subjects as the control group. The PON1(192) phenotype was predicted from examination of the two-dimensional plot of hydrolysis rates of diazoxon vs. paraoxon and by using the antimode of the histogram of the ratio of diazoxonase/paraoxonase activity. Results: The PON1Q192R phenotype frequencies in 113 patients with occlusion) 50% (coronary artery disease-positive, CAD+ group) vs. control population were as follows: QQ (0.552 vs. 0.510), QR (0.382 vs. 0.408) and RR (0.066 vs. 0.082); chi(2) = 0.414, p = 0.813. We found lower paraoxonase (POase) and diazoxonase (DZOase) activities in the CAD+ patients when compared to the control population. According to logistic regression analysis, POase activity was a better predictor of coronary disease onset compared with DZOase activity measurements and PON1Q192R phenotyping. Conclusions: We conclude that enzyme activity (within a particular phenotypic group) is more important than phenotype alone in predicting susceptibility to coronary artery disease.", publisher = "Walter de Gruyter Gmbh, Berlin", journal = "Clinical Chemistry and Laboratory Medicine", title = "Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population", volume = "44", number = "10", pages = "1206-1213", doi = "10.1515/CCLM.2006.216" }
Kotur-Stevuljević, J., Spasić, S., Stefanović, A., Zeljković, A., Bogavac-Stanojević, N., Kalimanovska-Oštrić, D., Spasojević-Kalimanovska, V.,& Jelić-Ivanović, Z.. (2006). Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population. in Clinical Chemistry and Laboratory Medicine Walter de Gruyter Gmbh, Berlin., 44(10), 1206-1213. https://doi.org/10.1515/CCLM.2006.216
Kotur-Stevuljević J, Spasić S, Stefanović A, Zeljković A, Bogavac-Stanojević N, Kalimanovska-Oštrić D, Spasojević-Kalimanovska V, Jelić-Ivanović Z. Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population. in Clinical Chemistry and Laboratory Medicine. 2006;44(10):1206-1213. doi:10.1515/CCLM.2006.216 .
Kotur-Stevuljević, Jelena, Spasić, Slavica, Stefanović, Aleksandra, Zeljković, Aleksandra, Bogavac-Stanojević, Nataša, Kalimanovska-Oštrić, Dimitra, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, "Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population" in Clinical Chemistry and Laboratory Medicine, 44, no. 10 (2006):1206-1213, https://doi.org/10.1515/CCLM.2006.216 . .