TY  - JOUR
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4471
AB  - Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia
VL  - 14
DO  - 10.3389/fmicb.2023.1094184
ER  - 

@article{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
abstract = "Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia",
volume = "14",
doi = "10.3389/fmicb.2023.1094184"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology
Frontiers Media S.A.., 14.
https://doi.org/10.3389/fmicb.2023.1094184

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology. 2023;14.
doi:10.3389/fmicb.2023.1094184 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia" in Frontiers in Microbiology, 14 (2023),
https://doi.org/10.3389/fmicb.2023.1094184 . .

TY  - CONF
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena M.
AU  - Arsenović-Ranin, Nevena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5133
AB  - Colistin is used as a “last resort” drug in the treatment
of carbapenem-resistant Acinetobacter baumannii
infections. Though not the most convenient option due to
the serious side effects, this antibiotic can be very efficient
if administered properly (Garnacho-Montero and Timsit,
2019). However, for several years now, we are witnessing
a steady emergence of colistin-resistant (ColR) strains, with
the constant threat of a sudden burst worldwide (Nowak et
al., 2017). Having in mind that these strains are usually
non-susceptible to any of the existing treatments, i.e.
pandrug-resistant (PDR), the development of new
therapeutic strategies is desperately needed.
In the present study, we showed that colistin
susceptibility of ColR A. baumannii strains can be fully
restored in the presence of very low amounts of selenium
nanoparticles (SeNPs). To demonstrate potent synergistic
interactions between colistin and SeNPs, we performed
checkerboard and time-kill analyses.
PB  - Macedonian Pharmaceutical Association
C3  - Macedonian Pharmaceutical Bulletin
T1  - Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin
VL  - 68
IS  - Suppl. 1
SP  - 295
EP  - 296
DO  - 10.33320/maced.pharm.bull.2022.68.03.142
ER  - 

@conference{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena M. and Arsenović-Ranin, Nevena and Milenković, Marina",
year = "2022",
abstract = "Colistin is used as a “last resort” drug in the treatment
of carbapenem-resistant Acinetobacter baumannii
infections. Though not the most convenient option due to
the serious side effects, this antibiotic can be very efficient
if administered properly (Garnacho-Montero and Timsit,
2019). However, for several years now, we are witnessing
a steady emergence of colistin-resistant (ColR) strains, with
the constant threat of a sudden burst worldwide (Nowak et
al., 2017). Having in mind that these strains are usually
non-susceptible to any of the existing treatments, i.e.
pandrug-resistant (PDR), the development of new
therapeutic strategies is desperately needed.
In the present study, we showed that colistin
susceptibility of ColR A. baumannii strains can be fully
restored in the presence of very low amounts of selenium
nanoparticles (SeNPs). To demonstrate potent synergistic
interactions between colistin and SeNPs, we performed
checkerboard and time-kill analyses.",
publisher = "Macedonian Pharmaceutical Association",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin",
volume = "68",
number = "Suppl. 1",
pages = "295-296",
doi = "10.33320/maced.pharm.bull.2022.68.03.142"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M. M., Arsenović-Ranin, N.,& Milenković, M.. (2022). Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(Suppl. 1), 295-296.
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović MM, Arsenović-Ranin N, Milenković M. Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin. 2022;68(Suppl. 1):295-296.
doi:10.33320/maced.pharm.bull.2022.68.03.142 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena M., Arsenović-Ranin, Nevena, Milenković, Marina, "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin" in Macedonian Pharmaceutical Bulletin, 68, no. Suppl. 1 (2022):295-296,
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Malešević, Milka
AU  - Vasiljević, Zorica
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4370
AB  - Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.
PB  - Springer Science and Business Media B.V.
T2  - Folia Microbiologica
T1  - Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene
VL  - n/d
DO  - 10.1007/s12223-022-01026-8
ER  - 

@article{
author = "Filipić, Brankica and Malešević, Milka and Vasiljević, Zorica and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "Folia Microbiologica",
title = "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene",
volume = "n/d",
doi = "10.1007/s12223-022-01026-8"
}

Filipić, B., Malešević, M., Vasiljević, Z., Novović, K., Kojić, M.,& Jovčić, B.. (2022). Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica
Springer Science and Business Media B.V.., n/d.
https://doi.org/10.1007/s12223-022-01026-8

Filipić B, Malešević M, Vasiljević Z, Novović K, Kojić M, Jovčić B. Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica. 2022;n/d.
doi:10.1007/s12223-022-01026-8 .

Filipić, Brankica, Malešević, Milka, Vasiljević, Zorica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene" in Folia Microbiologica, n/d (2022),
https://doi.org/10.1007/s12223-022-01026-8 . .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4177
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
VL  - 133
IS  - 3
SP  - 1197
SP  - 1197
EP  - 1206
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
volume = "133",
number = "3",
pages = "1197-1197-1206",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - CONF
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Jovčić, Branko
AU  - Kojić, Milan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5118
AB  - BACKGROUND
From the start of COVID-19 pandemic, many patients suffering severe clinical presentation of COVID-19 and 
acute respiratory failure have been administered to intensive care units (ICUs). Need for noninvasive or invasive 
mechanical ventilation represents a high-risk factor for these patients to develop Acinetobacter baumannii 
superinfection.
OBJECTIVES
The main goal of this study was to examine virulence potential of A. baumannii isolates recovered from 
COVID-19 patients admitted to ICU as well as to compare differences in virulence among isolates originated 
from male and female patients or from different sample types.
METHODS
64 A. baumannii isolates were recovered from COVID-19 patients from December 2020 to February 2021. 
The isolates were originated from different sample types: blood, tip of the central venous catheter, tracheal 
aspirate, tip of the aspirator and sputum. Genetic relatedness of ApaI digested A. baumannii isolates, 
was determined by pulsed-field gel electrophoresis (PFGE). Affinity to mucin binding was tested using in vitro 
model and isolates were examined for two types of motilities: swarming and twitching.
PB  - Federation of European Microbiological Societies
PB  - Serbian Society for Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
T1  - Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients
SP  - 96
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5118
ER  - 

@conference{
author = "Filipić, Brankica and Novović, Katarina and Kuzmanović Nedeljković, Snežana and Jovčić, Branko and Kojić, Milan",
year = "2022",
abstract = "BACKGROUND
From the start of COVID-19 pandemic, many patients suffering severe clinical presentation of COVID-19 and 
acute respiratory failure have been administered to intensive care units (ICUs). Need for noninvasive or invasive 
mechanical ventilation represents a high-risk factor for these patients to develop Acinetobacter baumannii 
superinfection.
OBJECTIVES
The main goal of this study was to examine virulence potential of A. baumannii isolates recovered from 
COVID-19 patients admitted to ICU as well as to compare differences in virulence among isolates originated 
from male and female patients or from different sample types.
METHODS
64 A. baumannii isolates were recovered from COVID-19 patients from December 2020 to February 2021. 
The isolates were originated from different sample types: blood, tip of the central venous catheter, tracheal 
aspirate, tip of the aspirator and sputum. Genetic relatedness of ApaI digested A. baumannii isolates, 
was determined by pulsed-field gel electrophoresis (PFGE). Affinity to mucin binding was tested using in vitro 
model and isolates were examined for two types of motilities: swarming and twitching.",
publisher = "Federation of European Microbiological Societies, Serbian Society for Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book",
title = "Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5118"
}

Filipić, B., Novović, K., Kuzmanović Nedeljković, S., Jovčić, B.,& Kojić, M.. (2022). Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
Federation of European Microbiological Societies., 96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5118

Filipić B, Novović K, Kuzmanović Nedeljković S, Jovčić B, Kojić M. Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book. 2022;:96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5118 .

Filipić, Brankica, Novović, Katarina, Kuzmanović Nedeljković, Snežana, Jovčić, Branko, Kojić, Milan, "Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book (2022):96-96,
https://hdl.handle.net/21.15107/rcub_farfar_5118 .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Miljković, Marija
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Studholme, David
AU  - Kojić, Milan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3729
AB  - Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis bv. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid pS74 is EPS production, while plasmid pS127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid pS127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.
PB  - Elsevier B.V.
T2  - International Journal of Food Microbiology
T1  - The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties
VL  - 337
DO  - 10.1016/j.ijfoodmicro.2020.108935
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Miljković, Marija and Jovčić, Branko and Filipić, Brankica and Studholme, David and Kojić, Milan",
year = "2021",
abstract = "Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis bv. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid pS74 is EPS production, while plasmid pS127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid pS127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Food Microbiology",
title = "The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties",
volume = "337",
doi = "10.1016/j.ijfoodmicro.2020.108935"
}

Malešević, M., Stanisavljević, N., Miljković, M., Jovčić, B., Filipić, B., Studholme, D.,& Kojić, M.. (2021). The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology
Elsevier B.V.., 337.
https://doi.org/10.1016/j.ijfoodmicro.2020.108935

Malešević M, Stanisavljević N, Miljković M, Jovčić B, Filipić B, Studholme D, Kojić M. The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology. 2021;337.
doi:10.1016/j.ijfoodmicro.2020.108935 .

Malešević, Milka, Stanisavljević, Nemanja, Miljković, Marija, Jovčić, Branko, Filipić, Brankica, Studholme, David, Kojić, Milan, "The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties" in International Journal of Food Microbiology, 337 (2021),
https://doi.org/10.1016/j.ijfoodmicro.2020.108935 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Obradović, Mina
AU  - O’Connor, Paula M.
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3932
AB  - Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.
PB  - Springer Nature
T2  - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
T1  - C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae
VL  - 114
SP  - 1595
EP  - 1607
DO  - 10.1007/s10482-021-01626-3
ER  - 

@article{
author = "Mirković, Nemanja and Obradović, Mina and O’Connor, Paula M. and Filipić, Brankica and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2021",
abstract = "Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.",
publisher = "Springer Nature",
journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",
title = "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae",
volume = "114",
pages = "1595-1607",
doi = "10.1007/s10482-021-01626-3"
}

Mirković, N., Obradović, M., O’Connor, P. M., Filipić, B., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2021). C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Springer Nature., 114, 1595-1607.
https://doi.org/10.1007/s10482-021-01626-3

Mirković N, Obradović M, O’Connor PM, Filipić B, Jovčić B, Cotter PD, Kojić M. C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2021;114:1595-1607.
doi:10.1007/s10482-021-01626-3 .

Mirković, Nemanja, Obradović, Mina, O’Connor, Paula M., Filipić, Brankica, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae" in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 114 (2021):1595-1607,
https://doi.org/10.1007/s10482-021-01626-3 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Studholme, David
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3631
AB  - Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.
PB  - NLM (Medline)
T2  - Journal of water and health
T1  - Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments
VL  - 18
IS  - 3
SP  - 383
EP  - 397
DO  - 10.2166/wh.2020.227
ER  - 

@article{
author = "Filipić, Brankica and Novović, Katarina and Studholme, David and Malešević, Milka and Mirković, Nemanja and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.",
publisher = "NLM (Medline)",
journal = "Journal of water and health",
title = "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments",
volume = "18",
number = "3",
pages = "383-397",
doi = "10.2166/wh.2020.227"
}

Filipić, B., Novović, K., Studholme, D., Malešević, M., Mirković, N., Kojić, M.,& Jovčić, B.. (2020). Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health
NLM (Medline)., 18(3), 383-397.
https://doi.org/10.2166/wh.2020.227

Filipić B, Novović K, Studholme D, Malešević M, Mirković N, Kojić M, Jovčić B. Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health. 2020;18(3):383-397.
doi:10.2166/wh.2020.227 .

Filipić, Brankica, Novović, Katarina, Studholme, David, Malešević, Milka, Mirković, Nemanja, Kojić, Milan, Jovčić, Branko, "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments" in Journal of water and health, 18, no. 3 (2020):383-397,
https://doi.org/10.2166/wh.2020.227 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Velhner, Maja
AU  - Todorović, Dalibor
AU  - Matović, Kazimir
AU  - Rašić, Zoran
AU  - Nikolić, Sonja
AU  - Kiškarolj, Ferenc
AU  - Kojić, Milan
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3760
AB  - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
PB  - MDPI AG
T2  - Antibiotics
T1  - Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia
VL  - 9
IS  - 12
SP  - 1
EP  - 13
DO  - 10.3390/antibiotics9120886
ER  - 

@article{
author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rašić, Zoran and Nikolić, Sonja and Kiškarolj, Ferenc and Kojić, Milan",
year = "2020",
abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.",
publisher = "MDPI AG",
journal = "Antibiotics",
title = "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia",
volume = "9",
number = "12",
pages = "1-13",
doi = "10.3390/antibiotics9120886"
}

Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rašić, Z., Nikolić, S., Kiškarolj, F.,& Kojić, M.. (2020). Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics
MDPI AG., 9(12), 1-13.
https://doi.org/10.3390/antibiotics9120886

Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rašić Z, Nikolić S, Kiškarolj F, Kojić M. Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics. 2020;9(12):1-13.
doi:10.3390/antibiotics9120886 .

Jovčić, Branko, Novović, Katarina, Filipić, Brankica, Velhner, Maja, Todorović, Dalibor, Matović, Kazimir, Rašić, Zoran, Nikolić, Sonja, Kiškarolj, Ferenc, Kojić, Milan, "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia" in Antibiotics, 9, no. 12 (2020):1-13,
https://doi.org/10.3390/antibiotics9120886 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Jovanović, Sofija
AU  - O'Connor, Paula M.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Dinić, Miroslav
AU  - Studholme, David John
AU  - Fira, Đorđe
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3372
AB  - Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials
VL  - 14
IS  - 5
DO  - 10.1371/journal.pone.0216773
ER  - 

@article{
author = "Miljković, Marija and Jovanović, Sofija and O'Connor, Paula M. and Mirković, Nemanja and Jovčić, Branko and Filipić, Brankica and Dinić, Miroslav and Studholme, David John and Fira, Đorđe and Cotter, Paul D. and Kojić, Milan",
year = "2019",
abstract = "Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials",
volume = "14",
number = "5",
doi = "10.1371/journal.pone.0216773"
}

Miljković, M., Jovanović, S., O'Connor, P. M., Mirković, N., Jovčić, B., Filipić, B., Dinić, M., Studholme, D. J., Fira, Đ., Cotter, P. D.,& Kojić, M.. (2019). Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One
Public Library Science, San Francisco., 14(5).
https://doi.org/10.1371/journal.pone.0216773

Miljković M, Jovanović S, O'Connor PM, Mirković N, Jovčić B, Filipić B, Dinić M, Studholme DJ, Fira Đ, Cotter PD, Kojić M. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One. 2019;14(5).
doi:10.1371/journal.pone.0216773 .

Miljković, Marija, Jovanović, Sofija, O'Connor, Paula M., Mirković, Nemanja, Jovčić, Branko, Filipić, Brankica, Dinić, Miroslav, Studholme, David John, Fira, Đorđe, Cotter, Paul D., Kojić, Milan, "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials" in PLoS One, 14, no. 5 (2019),
https://doi.org/10.1371/journal.pone.0216773 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malesević, Milka
AU  - Filipić, Brankica
AU  - Mirković, Nemanja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3313
AB  - Pseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, rpoS and psrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in rpoS and psrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.
PB  - Springer, New York
T2  - Current Microbiology
T1  - PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.
VL  - 76
IS  - 3
SP  - 320
EP  - 328
DO  - 10.1007/s00284-019-01626-7
ER  - 

@article{
author = "Novović, Katarina and Malesević, Milka and Filipić, Brankica and Mirković, Nemanja and Miljković, Marija and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, rpoS and psrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in rpoS and psrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.",
publisher = "Springer, New York",
journal = "Current Microbiology",
title = "PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.",
volume = "76",
number = "3",
pages = "320-328",
doi = "10.1007/s00284-019-01626-7"
}

Novović, K., Malesević, M., Filipić, B., Mirković, N., Miljković, M., Kojić, M.,& Jovčić, B.. (2019). PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.. in Current Microbiology
Springer, New York., 76(3), 320-328.
https://doi.org/10.1007/s00284-019-01626-7

Novović K, Malesević M, Filipić B, Mirković N, Miljković M, Kojić M, Jovčić B. PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.. in Current Microbiology. 2019;76(3):320-328.
doi:10.1007/s00284-019-01626-7 .

Novović, Katarina, Malesević, Milka, Filipić, Brankica, Mirković, Nemanja, Miljković, Marija, Kojić, Milan, Jovčić, Branko, "PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp." in Current Microbiology, 76, no. 3 (2019):320-328,
https://doi.org/10.1007/s00284-019-01626-7 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Di Lorenzo, Flaviana
AU  - Filipić, Brankica
AU  - Stanisavljević, Nemanja
AU  - Novović, Katarina
AU  - Senerovic, Lidija
AU  - Polović, Natalija
AU  - Molinaro, Antonio
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3538
AB  - Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.
PB  - NatureResearch
T2  - Scientific Reports
T1  - Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones
VL  - 9
IS  - 1
DO  - 10.1038/s41598-019-52955-3
ER  - 

@article{
author = "Malešević, Milka and Di Lorenzo, Flaviana and Filipić, Brankica and Stanisavljević, Nemanja and Novović, Katarina and Senerovic, Lidija and Polović, Natalija and Molinaro, Antonio and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.",
publisher = "NatureResearch",
journal = "Scientific Reports",
title = "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones",
volume = "9",
number = "1",
doi = "10.1038/s41598-019-52955-3"
}

Malešević, M., Di Lorenzo, F., Filipić, B., Stanisavljević, N., Novović, K., Senerovic, L., Polović, N., Molinaro, A., Kojić, M.,& Jovčić, B.. (2019). Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports
NatureResearch., 9(1).
https://doi.org/10.1038/s41598-019-52955-3

Malešević M, Di Lorenzo F, Filipić B, Stanisavljević N, Novović K, Senerovic L, Polović N, Molinaro A, Kojić M, Jovčić B. Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports. 2019;9(1).
doi:10.1038/s41598-019-52955-3 .

Malešević, Milka, Di Lorenzo, Flaviana, Filipić, Brankica, Stanisavljević, Nemanja, Novović, Katarina, Senerovic, Lidija, Polović, Natalija, Molinaro, Antonio, Kojić, Milan, Jovčić, Branko, "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones" in Scientific Reports, 9, no. 1 (2019),
https://doi.org/10.1038/s41598-019-52955-3 . .

TY  - JOUR
AU  - Malesević, Milka
AU  - Mirković, Nemanja
AU  - Lozo, Jelena
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3333
AB  - 16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Geomicrobiology Journal
T1  - Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population
VL  - 36
IS  - 3
SP  - 261
EP  - 270
DO  - 10.1080/01490451.2018.1550128
ER  - 

@article{
author = "Malesević, Milka and Mirković, Nemanja and Lozo, Jelena and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Geomicrobiology Journal",
title = "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population",
volume = "36",
number = "3",
pages = "261-270",
doi = "10.1080/01490451.2018.1550128"
}

Malesević, M., Mirković, N., Lozo, J., Novović, K., Filipić, B., Kojić, M.,& Jovčić, B.. (2019). Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal
Taylor & Francis Inc, Philadelphia., 36(3), 261-270.
https://doi.org/10.1080/01490451.2018.1550128

Malesević M, Mirković N, Lozo J, Novović K, Filipić B, Kojić M, Jovčić B. Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal. 2019;36(3):261-270.
doi:10.1080/01490451.2018.1550128 .

Malesević, Milka, Mirković, Nemanja, Lozo, Jelena, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Jovčić, Branko, "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population" in Geomicrobiology Journal, 36, no. 3 (2019):261-270,
https://doi.org/10.1080/01490451.2018.1550128 . .

TY  - JOUR
AU  - Lilić, Branislav
AU  - Filipić, Brankica
AU  - Malesević, Milka
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3298
AB  - The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis. The influence of an H+ conductor cyanide m-chlorophenyl hydrazone (CCCP) and a resistance-nodulation-division-type efflux pump inhibitor phenylalanine-arginine beta-naphthylamide (PAN) on minimal inhibitory concentration (MIC) value was evaluated by broth microdilution. The presence of the plasmid-mediated qnrA, qnrB, qnrC, qnrS, and aac-(6)-Ib-cr genes was investigated by PCR and sequencing. Achromobacter spp. isolates that were resistant or intermediately resistant to fluoroquinolones in disk diffusion tests (44/98) were subjected to microdilution. As a result, 20/98 isolates were confirmed to be resistant to ciprofloxacin while 10/98 was resistant to levofloxacin. CCCP decreased twofold MIC value for ciprofloxacin in six isolates and more than 16 times in one isolate, while MIC value for levofloxacin was decreased in all isolates (twofold to more than eightfold). Fluoroquinolone-resistant isolates were identified as A. xylosoxidans with the nrdA gene sequencing. PFGE revealed that resistant isolates belonged to seven different genotypes. Ten isolates belonging to four genotypes were positive for the aac-(6)-Ib-cr gene. Although resistance to fluoroquinolones was not widespread among analyzed isolates, detected contribution of efflux pumps and the presence of the aac-(6)-Ib-cr gene present a platform for emergence of more resistant strains.
PB  - Springer, Dordrecht
T2  - Folia Microbiologica
T1  - Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates
VL  - 64
IS  - 2
SP  - 153
EP  - 159
DO  - 10.1007/s12223-018-0639-7
ER  - 

@article{
author = "Lilić, Branislav and Filipić, Brankica and Malesević, Milka and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis. The influence of an H+ conductor cyanide m-chlorophenyl hydrazone (CCCP) and a resistance-nodulation-division-type efflux pump inhibitor phenylalanine-arginine beta-naphthylamide (PAN) on minimal inhibitory concentration (MIC) value was evaluated by broth microdilution. The presence of the plasmid-mediated qnrA, qnrB, qnrC, qnrS, and aac-(6)-Ib-cr genes was investigated by PCR and sequencing. Achromobacter spp. isolates that were resistant or intermediately resistant to fluoroquinolones in disk diffusion tests (44/98) were subjected to microdilution. As a result, 20/98 isolates were confirmed to be resistant to ciprofloxacin while 10/98 was resistant to levofloxacin. CCCP decreased twofold MIC value for ciprofloxacin in six isolates and more than 16 times in one isolate, while MIC value for levofloxacin was decreased in all isolates (twofold to more than eightfold). Fluoroquinolone-resistant isolates were identified as A. xylosoxidans with the nrdA gene sequencing. PFGE revealed that resistant isolates belonged to seven different genotypes. Ten isolates belonging to four genotypes were positive for the aac-(6)-Ib-cr gene. Although resistance to fluoroquinolones was not widespread among analyzed isolates, detected contribution of efflux pumps and the presence of the aac-(6)-Ib-cr gene present a platform for emergence of more resistant strains.",
publisher = "Springer, Dordrecht",
journal = "Folia Microbiologica",
title = "Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates",
volume = "64",
number = "2",
pages = "153-159",
doi = "10.1007/s12223-018-0639-7"
}

Lilić, B., Filipić, B., Malesević, M., Novović, K., Vasiljević, Z., Kojić, M.,& Jovčić, B.. (2019). Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates. in Folia Microbiologica
Springer, Dordrecht., 64(2), 153-159.
https://doi.org/10.1007/s12223-018-0639-7

Lilić B, Filipić B, Malesević M, Novović K, Vasiljević Z, Kojić M, Jovčić B. Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates. in Folia Microbiologica. 2019;64(2):153-159.
doi:10.1007/s12223-018-0639-7 .

Lilić, Branislav, Filipić, Brankica, Malesević, Milka, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, "Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates" in Folia Microbiologica, 64, no. 2 (2019):153-159,
https://doi.org/10.1007/s12223-018-0639-7 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Malesević, Milka
AU  - Filipić, Brankica
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3192
AB  - Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
DO  - 10.1155/2018/5657085
ER  - 

@article{
author = "Miljković, Marija and Malesević, Milka and Filipić, Brankica and Vukotić, Goran and Kojić, Milan",
year = "2018",
abstract = "Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity",
doi = "10.1155/2018/5657085"
}

Miljković, M., Malesević, M., Filipić, B., Vukotić, G.,& Kojić, M.. (2018). LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International
Hindawi Ltd, London..
https://doi.org/10.1155/2018/5657085

Miljković M, Malesević M, Filipić B, Vukotić G, Kojić M. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International. 2018;.
doi:10.1155/2018/5657085 .

Miljković, Marija, Malesević, Milka, Filipić, Brankica, Vukotić, Goran, Kojić, Milan, "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity" in Biomed Research International (2018),
https://doi.org/10.1155/2018/5657085 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Malesević, Milka
AU  - Vasiljević, Zorica
AU  - Lukić, Jovanka
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2999
AB  - Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
VL  - 7
DO  - 10.3389/fcimb.2017.00224
ER  - 

@article{
author = "Filipić, Brankica and Malesević, Milka and Vasiljević, Zorica and Lukić, Jovanka and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2017",
abstract = "Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin",
volume = "7",
doi = "10.3389/fcimb.2017.00224"
}

Filipić, B., Malesević, M., Vasiljević, Z., Lukić, J., Novović, K., Kojić, M.,& Jovčić, B.. (2017). Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fcimb.2017.00224

Filipić B, Malesević M, Vasiljević Z, Lukić J, Novović K, Kojić M, Jovčić B. Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology. 2017;7.
doi:10.3389/fcimb.2017.00224 .

Filipić, Brankica, Malesević, Milka, Vasiljević, Zorica, Lukić, Jovanka, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin" in Frontiers in Cellular and Infection Microbiology, 7 (2017),
https://doi.org/10.3389/fcimb.2017.00224 . .

TY  - JOUR
AU  - Malesević, Milka
AU  - Vasiljević, Zorica
AU  - Sovtić, Aleksandar
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2974
AB  - Background: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). Methods: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed. Results: Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene. Conclusion: Variations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.
PB  - Biomed Central Ltd, London
T2  - Antimicrobial Resistance and Infection Control
T1  - Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients
VL  - 6
DO  - 10.1186/s13756-017-0215-y
ER  - 

@article{
author = "Malesević, Milka and Vasiljević, Zorica and Sovtić, Aleksandar and Filipić, Brankica and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2017",
abstract = "Background: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). Methods: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed. Results: Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene. Conclusion: Variations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.",
publisher = "Biomed Central Ltd, London",
journal = "Antimicrobial Resistance and Infection Control",
title = "Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients",
volume = "6",
doi = "10.1186/s13756-017-0215-y"
}

Malesević, M., Vasiljević, Z., Sovtić, A., Filipić, B., Novović, K., Kojić, M.,& Jovčić, B.. (2017). Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients. in Antimicrobial Resistance and Infection Control
Biomed Central Ltd, London., 6.
https://doi.org/10.1186/s13756-017-0215-y

Malesević M, Vasiljević Z, Sovtić A, Filipić B, Novović K, Kojić M, Jovčić B. Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients. in Antimicrobial Resistance and Infection Control. 2017;6.
doi:10.1186/s13756-017-0215-y .

Malesević, Milka, Vasiljević, Zorica, Sovtić, Aleksandar, Filipić, Brankica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients" in Antimicrobial Resistance and Infection Control, 6 (2017),
https://doi.org/10.1186/s13756-017-0215-y . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Božić, Dragana
AU  - Draganić, Veselin
AU  - Lozo, Jelena
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Arsić, Biljana
AU  - Garalejić, Eliana
AU  - Đukić, Slobodanka
AU  - Stanković, Slavisa
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2583
AB  - Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167995
ER  - 

@article{
author = "Cirković, Ivana and Božić, Dragana and Draganić, Veselin and Lozo, Jelena and Berić, Tanja and Kojić, Milan and Arsić, Biljana and Garalejić, Eliana and Đukić, Slobodanka and Stanković, Slavisa",
year = "2016",
abstract = "Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167995"
}

Cirković, I., Božić, D., Draganić, V., Lozo, J., Berić, T., Kojić, M., Arsić, B., Garalejić, E., Đukić, S.,& Stanković, S.. (2016). Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167995

Cirković I, Božić D, Draganić V, Lozo J, Berić T, Kojić M, Arsić B, Garalejić E, Đukić S, Stanković S. Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One. 2016;11(12).
doi:10.1371/journal.pone.0167995 .

Cirković, Ivana, Božić, Dragana, Draganić, Veselin, Lozo, Jelena, Berić, Tanja, Kojić, Milan, Arsić, Biljana, Garalejić, Eliana, Đukić, Slobodanka, Stanković, Slavisa, "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates" in PLoS One, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167995 . .

TY  - JOUR
AU  - Kolundžić, Marina
AU  - Groznanić, Nađa
AU  - Stanojković, Tatjana
AU  - Milenković, Marina
AU  - Dinić, Miroslav
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Kundaković, Tatjana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2755
AB  - Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia
VL  - 18
IS  - 6
SP  - 469
EP  - 476
DO  - 10.1615/IntJMedMushrooms.v18.i6.10
ER  - 

@article{
author = "Kolundžić, Marina and Groznanić, Nađa and Stanojković, Tatjana and Milenković, Marina and Dinić, Miroslav and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana",
year = "2016",
abstract = "Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia",
volume = "18",
number = "6",
pages = "469-476",
doi = "10.1615/IntJMedMushrooms.v18.i6.10"
}

Kolundžić, M., Groznanić, N., Stanojković, T., Milenković, M., Dinić, M., Golić, N., Kojić, M.,& Kundaković, T.. (2016). Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 18(6), 469-476.
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10

Kolundžić M, Groznanić N, Stanojković T, Milenković M, Dinić M, Golić N, Kojić M, Kundaković T. Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms. 2016;18(6):469-476.
doi:10.1615/IntJMedMushrooms.v18.i6.10 .

Kolundžić, Marina, Groznanić, Nađa, Stanojković, Tatjana, Milenković, Marina, Dinić, Miroslav, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana, "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia" in International Journal of Medicinal Mushrooms, 18, no. 6 (2016):469-476,
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10 . .

TY  - JOUR
AU  - Brđanin, Stanislava
AU  - Bogdanović, Nemanja
AU  - Kolundžić, Marina
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Kundaković, Tatjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2349
AB  - The commercial samples of aerial parts of Origanum vulgare L. and Ocimum basilicum L. (Lamiaceae) were tested for antimicrobial activity. The activity of the extracts with different polarity was tested against a panel of microorganisms, including laboratory strain Helicobacter pylori NCTC 12868. The tested extracts showed a moderate activity. The extracts of O. vulgare were more active against bacteria, especially against Gram positive bacteria with the minimal inhibitory concentration (MIC) between 62.5 and 125 μg/mL, than the extracts of O. basilicum which were active against Candida albicans (MIC 125 μg/mL). Cyclohexane extract of O. vulgare did not show any activity against tested H. pylori, while all other tested extracts were active with MICs between 250 μg/mL and 500 μg/mL. Identified and quantified rosmarinic acid and other polar compounds could be active antibacterial compounds in these spices.
AB  - Komercijalni uzorci nadzemnih delova Origanum vulgare L. i Ocimum basilicum L. (Lamiaceae) su korišćeni za određivanje antimikrobne aktivnosti. Aktivnost ekstrakata različite polarnosti je testirana protiv izabranih mikroorganizama, uključujući laboratorijski soj Helicobacter pilori NCTC 12868. Testirani ekstrakti su imali umerenu aktivnost. Ekstrakti O. vulgare su bili aktivniji protiv bakterija, posebno Gram pozitivnih bakterija sa minimalnim inhibitornim koncentracijama (MIK) između 62,5 i 125 μg/mL, od ekstrakata O. basilicum koji su pokazali aktivnost protiv Candida albicans (MIK 125 μg/mL). Cikloheksanski ekstrakt O. vulgare nije pokazao antimikrobnu aktivnost na testirani H. pylori, dok su ostali testirani ekstrakti bili aktivni sa MIK vrednostima između 250 μg/mL i 500 μg/mL. Identifikovana i kvantifikovana ruzmarinska kiselina i druga polarna jedinjenja mogu predstavljati aktivna jedinjenja sa antibakterijskom aktivnošću u ovim začinima.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Advanced Technologies
T1  - Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts
T1  - Antimikrobna aktivnost ekstrakata origana (Origanum vulgare L.) i bosiljka (Ocimum basilicum L.)
VL  - 4
IS  - 2
SP  - 5
EP  - 10
DO  - 10.5937/savteh1502005B
ER  - 

@article{
author = "Brđanin, Stanislava and Bogdanović, Nemanja and Kolundžić, Marina and Milenković, Marina and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana",
year = "2015",
abstract = "The commercial samples of aerial parts of Origanum vulgare L. and Ocimum basilicum L. (Lamiaceae) were tested for antimicrobial activity. The activity of the extracts with different polarity was tested against a panel of microorganisms, including laboratory strain Helicobacter pylori NCTC 12868. The tested extracts showed a moderate activity. The extracts of O. vulgare were more active against bacteria, especially against Gram positive bacteria with the minimal inhibitory concentration (MIC) between 62.5 and 125 μg/mL, than the extracts of O. basilicum which were active against Candida albicans (MIC 125 μg/mL). Cyclohexane extract of O. vulgare did not show any activity against tested H. pylori, while all other tested extracts were active with MICs between 250 μg/mL and 500 μg/mL. Identified and quantified rosmarinic acid and other polar compounds could be active antibacterial compounds in these spices., Komercijalni uzorci nadzemnih delova Origanum vulgare L. i Ocimum basilicum L. (Lamiaceae) su korišćeni za određivanje antimikrobne aktivnosti. Aktivnost ekstrakata različite polarnosti je testirana protiv izabranih mikroorganizama, uključujući laboratorijski soj Helicobacter pilori NCTC 12868. Testirani ekstrakti su imali umerenu aktivnost. Ekstrakti O. vulgare su bili aktivniji protiv bakterija, posebno Gram pozitivnih bakterija sa minimalnim inhibitornim koncentracijama (MIK) između 62,5 i 125 μg/mL, od ekstrakata O. basilicum koji su pokazali aktivnost protiv Candida albicans (MIK 125 μg/mL). Cikloheksanski ekstrakt O. vulgare nije pokazao antimikrobnu aktivnost na testirani H. pylori, dok su ostali testirani ekstrakti bili aktivni sa MIK vrednostima između 250 μg/mL i 500 μg/mL. Identifikovana i kvantifikovana ruzmarinska kiselina i druga polarna jedinjenja mogu predstavljati aktivna jedinjenja sa antibakterijskom aktivnošću u ovim začinima.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Advanced Technologies",
title = "Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts, Antimikrobna aktivnost ekstrakata origana (Origanum vulgare L.) i bosiljka (Ocimum basilicum L.)",
volume = "4",
number = "2",
pages = "5-10",
doi = "10.5937/savteh1502005B"
}

Brđanin, S., Bogdanović, N., Kolundžić, M., Milenković, M., Golić, N., Kojić, M.,& Kundaković, T.. (2015). Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts. in Advanced Technologies
Univerzitet u Nišu - Tehnološki fakultet, Leskovac., 4(2), 5-10.
https://doi.org/10.5937/savteh1502005B

Brđanin S, Bogdanović N, Kolundžić M, Milenković M, Golić N, Kojić M, Kundaković T. Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts. in Advanced Technologies. 2015;4(2):5-10.
doi:10.5937/savteh1502005B .

Brđanin, Stanislava, Bogdanović, Nemanja, Kolundžić, Marina, Milenković, Marina, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana, "Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts" in Advanced Technologies, 4, no. 2 (2015):5-10,
https://doi.org/10.5937/savteh1502005B . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Popović, Dušanka
AU  - Tolinacki, Maja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2320
AB  - Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?
VL  - 6
DO  - 10.3389/fmicb.2015.00954
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Begović, Jelena and Filipić, Brankica and Popović, Dušanka and Tolinacki, Maja and Miljković, Marija and Kojić, Milan and Golić, Nataša",
year = "2015",
abstract = "Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?",
volume = "6",
doi = "10.3389/fmicb.2015.00954"
}

Terzić-Vidojević, A., Veljović, K., Begović, J., Filipić, B., Popović, D., Tolinacki, M., Miljković, M., Kojić, M.,& Golić, N.. (2015). Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fmicb.2015.00954

Terzić-Vidojević A, Veljović K, Begović J, Filipić B, Popović D, Tolinacki M, Miljković M, Kojić M, Golić N. Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00954 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Begović, Jelena, Filipić, Brankica, Popović, Dušanka, Tolinacki, Maja, Miljković, Marija, Kojić, Milan, Golić, Nataša, "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00954 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Golić, Nataša
AU  - Jovčić, Branko
AU  - Ćupić-Miladinović, Dejana
AU  - Soković, Svetlana
AU  - Popović, Dušanka
AU  - Kojić, Milan
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2483
AB  - Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry.
AB  - Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus
T1  - Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus
VL  - 69
IS  - 3-4
SP  - 271
EP  - 281
DO  - 10.2298/VETGL1504271F
ER  - 

@article{
author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Ćupić-Miladinović, Dejana and Soković, Svetlana and Popović, Dušanka and Kojić, Milan",
year = "2015",
abstract = "Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry., Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus, Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus",
volume = "69",
number = "3-4",
pages = "271-281",
doi = "10.2298/VETGL1504271F"
}

Filipić, B., Golić, N., Jovčić, B., Ćupić-Miladinović, D., Soković, S., Popović, D.,& Kojić, M.. (2015). Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 271-281.
https://doi.org/10.2298/VETGL1504271F

Filipić B, Golić N, Jovčić B, Ćupić-Miladinović D, Soković S, Popović D, Kojić M. Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik. 2015;69(3-4):271-281.
doi:10.2298/VETGL1504271F .

Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Ćupić-Miladinović, Dejana, Soković, Svetlana, Popović, Dušanka, Kojić, Milan, "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus" in Veterinarski glasnik, 69, no. 3-4 (2015):271-281,
https://doi.org/10.2298/VETGL1504271F . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Nikolić, Katarina
AU  - Filipić, Slavica
AU  - Jovčić, Branko
AU  - Agbaba, Danica
AU  - Antić-Stanković, Jelena
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2232
AB  - The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study
VL  - 45
IS  - 3
SP  - 764
EP  - 771
DO  - 10.1016/j.jtice.2013.09.033
ER  - 

@article{
author = "Filipić, Brankica and Nikolić, Katarina and Filipić, Slavica and Jovčić, Branko and Agbaba, Danica and Antić-Stanković, Jelena and Kojić, Milan and Golić, Nataša",
year = "2014",
abstract = "The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study",
volume = "45",
number = "3",
pages = "764-771",
doi = "10.1016/j.jtice.2013.09.033"
}

Filipić, B., Nikolić, K., Filipić, S., Jovčić, B., Agbaba, D., Antić-Stanković, J., Kojić, M.,& Golić, N.. (2014). Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science BV, Amsterdam., 45(3), 764-771.
https://doi.org/10.1016/j.jtice.2013.09.033

Filipić B, Nikolić K, Filipić S, Jovčić B, Agbaba D, Antić-Stanković J, Kojić M, Golić N. Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers. 2014;45(3):764-771.
doi:10.1016/j.jtice.2013.09.033 .

Filipić, Brankica, Nikolić, Katarina, Filipić, Slavica, Jovčić, Branko, Agbaba, Danica, Antić-Stanković, Jelena, Kojić, Milan, Golić, Nataša, "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study" in Journal of the Taiwan Institute of Chemical Engineers, 45, no. 3 (2014):764-771,
https://doi.org/10.1016/j.jtice.2013.09.033 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Lepsanović, Zorica
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Kojić, Milan
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2173
AB  - New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.
PB  - Springer, Dordrecht
T2  - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
T1  - Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia
VL  - 105
IS  - 3
SP  - 613
EP  - 618
DO  - 10.1007/s10482-013-0094-z
ER  - 

@article{
author = "Jovčić, Branko and Lepsanović, Zorica and Begović, Jelena and Filipić, Brankica and Kojić, Milan",
year = "2014",
abstract = "New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.",
publisher = "Springer, Dordrecht",
journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",
title = "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia",
volume = "105",
number = "3",
pages = "613-618",
doi = "10.1007/s10482-013-0094-z"
}

Jovčić, B., Lepsanović, Z., Begović, J., Filipić, B.,& Kojić, M.. (2014). Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Springer, Dordrecht., 105(3), 613-618.
https://doi.org/10.1007/s10482-013-0094-z

Jovčić B, Lepsanović Z, Begović J, Filipić B, Kojić M. Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2014;105(3):613-618.
doi:10.1007/s10482-013-0094-z .

Jovčić, Branko, Lepsanović, Zorica, Begović, Jelena, Filipić, Brankica, Kojić, Milan, "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia" in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 105, no. 3 (2014):613-618,
https://doi.org/10.1007/s10482-013-0094-z . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Nikolić, Milica
AU  - Tolinacki, Maja
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2070
AB  - Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.
PB  - Springer, New York
T2  - Microbial Ecology
T1  - Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa
VL  - 68
IS  - 3
SP  - 633
EP  - 644
DO  - 10.1007/s00248-014-0426-1
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Nikolić, Milica and Tolinacki, Maja and Kojić, Milan and Begović, Jelena",
year = "2014",
abstract = "Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.",
publisher = "Springer, New York",
journal = "Microbial Ecology",
title = "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa",
volume = "68",
number = "3",
pages = "633-644",
doi = "10.1007/s00248-014-0426-1"
}

Lukić, J., Strahinić, I., Milenković, M., Nikolić, M., Tolinacki, M., Kojić, M.,& Begović, J.. (2014). Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology
Springer, New York., 68(3), 633-644.
https://doi.org/10.1007/s00248-014-0426-1

Lukić J, Strahinić I, Milenković M, Nikolić M, Tolinacki M, Kojić M, Begović J. Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology. 2014;68(3):633-644.
doi:10.1007/s00248-014-0426-1 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Nikolić, Milica, Tolinacki, Maja, Kojić, Milan, Begović, Jelena, "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa" in Microbial Ecology, 68, no. 3 (2014):633-644,
https://doi.org/10.1007/s00248-014-0426-1 . .