TY  - CHAP
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5184
AB  - Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.
PB  - Elsevier
T2  - Olives and Olive Oil in Health and Disease Prevention
T1  - Olive leaf, DNA damage and chelation therapy
SP  - 457
EP  - 469
DO  - 10.1016/B978-0-12-819528-4.00021-3
ER  - 

@inbook{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.",
publisher = "Elsevier",
journal = "Olives and Olive Oil in Health and Disease Prevention",
booktitle = "Olive leaf, DNA damage and chelation therapy",
pages = "457-469",
doi = "10.1016/B978-0-12-819528-4.00021-3"
}

Čabarkapa-Pirković, A., Živković, L., Dekanski, D., Topalović, D.,& Spremo-Potparević, B.. (2021). Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention
Elsevier., 457-469.
https://doi.org/10.1016/B978-0-12-819528-4.00021-3

Čabarkapa-Pirković A, Živković L, Dekanski D, Topalović D, Spremo-Potparević B. Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention. 2021;:457-469.
doi:10.1016/B978-0-12-819528-4.00021-3 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Spremo-Potparević, Biljana, "Olive leaf, DNA damage and chelation therapy" in Olives and Olive Oil in Health and Disease Prevention (2021):457-469,
https://doi.org/10.1016/B978-0-12-819528-4.00021-3 . .

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920
AB  - Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
AB  - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
T1  - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
VL  - 71
IS  - 3
SP  - 197
EP  - 206
DO  - 10.5937/arhfarm71‐31060
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada",
year = "2021",
abstract = "Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka",
volume = "71",
number = "3",
pages = "197-206",
doi = "10.5937/arhfarm71‐31060"
}

Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206.
https://doi.org/10.5937/arhfarm71‐31060

Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206.
doi:10.5937/arhfarm71‐31060 .

Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206,
https://doi.org/10.5937/arhfarm71‐31060 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes-Hernández, Tamara
AU  - Zlatković-Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
PB  - Wiley
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.",
publisher = "Wiley",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry
Wiley., 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3165
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 

@article{
author = "Dekanski, Dragana and Potparević, Biljana and Bajić, Vladan and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna and Nedeljković, Jovan",
year = "2018",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}

Dekanski, D., Potparević, B., Bajić, V., Živković, L., Topalović, D., Sredojević, D., Lazić, V.,& Nedeljković, J.. (2018). Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064

Dekanski D, Potparević B, Bajić V, Živković L, Topalović D, Sredojević D, Lazić V, Nedeljković J. Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology. 2018;115:42-48.
doi:10.1016/j.fct.2018.02.064 .

Dekanski, Dragana, Potparević, Biljana, Bajić, Vladan, Živković, Lada, Topalović, Dijana, Sredojević, Dušan, Lazić, Vesna, Nedeljković, Jovan, "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid" in Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 . .

TY  - CONF
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5150
AB  - Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...
PB  - National Health Institute Dr. Ricardo Jorge
PB  - Institute of Public Health of the University of Porto (ISPUP)
C3  - 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
T1  - Evaluation of adrenaline-induced DNA damage in vitro by comet assay
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5150
ER  - 

@conference{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...",
publisher = "National Health Institute Dr. Ricardo Jorge, Institute of Public Health of the University of Porto (ISPUP)",
journal = "4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book",
title = "Evaluation of adrenaline-induced DNA damage in vitro by comet assay",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5150"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
National Health Institute Dr. Ricardo Jorge., 135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150

Topalović D, Dekanski D, Spremo-Potparević B, Đelić N, Bajić V, Živković L. Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book. 2018;:135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Evaluation of adrenaline-induced DNA damage in vitro by comet assay" in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book (2018):135-135,
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

TY  - JOUR
AU  - Grujić, Branka
AU  - Vuković, Zdenka
AU  - Veljković-Giga, Jelena
AU  - Ibrić, Svetlana
AU  - Dekanski, Dragana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3217
AB  - Nutrition and dietary supplements have a significant role in the prevention and treatment of cardiovascular disease. Olive and vine leaves, thanks to their constituents, represent powerful natural antioxidants exhibiting cardioprotective activity. High concentrations of active ingredients can be provided by means of extraction. Dry herbal extracts are highly sensitive to moisture and elevated temperature and from the pharmaceutical and technological point of view these are raw materials with inadequate technological properties. The aim of this study was to investigate and to compare the influence of different excipients in capsule formulation of these two dry extracts i.e. the selection of excipients that will ensure appropriate critical process parameters in the manufacturing procedure. The monitored quality characteristics include flowability, bulk density and tapped density that are critical for technological feasibility of the capsule-filling operation. The effects of excipients on the above parameters have been experimentally studied and the appropriate capsule formulations have been developed based on the obtained results, which will ensure homogeneity and stability of the preparation.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - An investigation into the effects of excipients on quality characteristics of a dry herbal extract containing capsule
VL  - 72
IS  - 4
SP  - 183
EP  - 189
DO  - 10.2298/HEMIND180228012G
ER  - 

@article{
author = "Grujić, Branka and Vuković, Zdenka and Veljković-Giga, Jelena and Ibrić, Svetlana and Dekanski, Dragana",
year = "2018",
abstract = "Nutrition and dietary supplements have a significant role in the prevention and treatment of cardiovascular disease. Olive and vine leaves, thanks to their constituents, represent powerful natural antioxidants exhibiting cardioprotective activity. High concentrations of active ingredients can be provided by means of extraction. Dry herbal extracts are highly sensitive to moisture and elevated temperature and from the pharmaceutical and technological point of view these are raw materials with inadequate technological properties. The aim of this study was to investigate and to compare the influence of different excipients in capsule formulation of these two dry extracts i.e. the selection of excipients that will ensure appropriate critical process parameters in the manufacturing procedure. The monitored quality characteristics include flowability, bulk density and tapped density that are critical for technological feasibility of the capsule-filling operation. The effects of excipients on the above parameters have been experimentally studied and the appropriate capsule formulations have been developed based on the obtained results, which will ensure homogeneity and stability of the preparation.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "An investigation into the effects of excipients on quality characteristics of a dry herbal extract containing capsule",
volume = "72",
number = "4",
pages = "183-189",
doi = "10.2298/HEMIND180228012G"
}

Grujić, B., Vuković, Z., Veljković-Giga, J., Ibrić, S.,& Dekanski, D.. (2018). An investigation into the effects of excipients on quality characteristics of a dry herbal extract containing capsule. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 72(4), 183-189.
https://doi.org/10.2298/HEMIND180228012G

Grujić B, Vuković Z, Veljković-Giga J, Ibrić S, Dekanski D. An investigation into the effects of excipients on quality characteristics of a dry herbal extract containing capsule. in Hemijska industrija. 2018;72(4):183-189.
doi:10.2298/HEMIND180228012G .

Grujić, Branka, Vuković, Zdenka, Veljković-Giga, Jelena, Ibrić, Svetlana, Dekanski, Dragana, "An investigation into the effects of excipients on quality characteristics of a dry herbal extract containing capsule" in Hemijska industrija, 72, no. 4 (2018):183-189,
https://doi.org/10.2298/HEMIND180228012G . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3131
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Potparević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3092
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Dekanski, Dragana and Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V., Dekanski, D., Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić V, Dekanski D, Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan, Dekanski, Dragana, Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - CONF
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Žukovec, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5186
AB  - Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5186
ER  - 

@conference{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Žukovec, Dijana and Dekanski, Dragana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5186"
}

Čabarkapa-Pirković, A., Živković, L., Žukovec, D., Dekanski, D., Bajić, V.,& Spremo-Potparević, B.. (2017). Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186

Čabarkapa-Pirković A, Živković L, Žukovec D, Dekanski D, Bajić V, Spremo-Potparević B. Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Žukovec, Dijana, Dekanski, Dragana, Bajić, Vladan, Spremo-Potparević, Biljana, "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):110-110,
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

TY  - CONF
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Borozan, Sunčica
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5127
AB  - Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5127
ER  - 

@conference{
author = "Topalović, Dijana and Živković, Lada and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Bajić, Vladan and Borozan, Sunčica and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5127"
}

Topalović, D., Živković, L., Čabarkapa-Pirković, A., Dekanski, D., Bajić, V., Borozan, S.,& Spremo-Potparević, B.. (2017). Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127

Topalović D, Živković L, Čabarkapa-Pirković A, Dekanski D, Bajić V, Borozan S, Spremo-Potparević B. Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127 .

Topalović, Dijana, Živković, Lada, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Bajić, Vladan, Borozan, Sunčica, Spremo-Potparević, Biljana, "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):58-58,
https://hdl.handle.net/21.15107/rcub_farfar_5127 .

TY  - JOUR
AU  - Janković, Milena
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2941
AB  - Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role.
AB  - Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro
T1  - Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro
VL  - 51
IS  - 2
SP  - 39
EP  - 45
DO  - 10.5937/mckg51-15559
ER  - 

@article{
author = "Janković, Milena and Živković, Lada and Pirković, Andrea and Topalović, Dijana and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role., Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro, Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro",
volume = "51",
number = "2",
pages = "39-45",
doi = "10.5937/mckg51-15559"
}

Janković, M., Živković, L., Pirković, A., Topalović, D., Dekanski, D., Bajić, V.,& Potparević, B.. (2017). Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 51(2), 39-45.
https://doi.org/10.5937/mckg51-15559

Janković M, Živković L, Pirković A, Topalović D, Dekanski D, Bajić V, Potparević B. Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis. 2017;51(2):39-45.
doi:10.5937/mckg51-15559 .

Janković, Milena, Živković, Lada, Pirković, Andrea, Topalović, Dijana, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro" in Medicinski časopis, 51, no. 2 (2017):39-45,
https://doi.org/10.5937/mckg51-15559 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2822
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 

@article{
author = "Pirković, Andrea and Dekanski, Dragana and Živković, Lada and Milanović-Čabarkapa, Mirjana and Bajić, Vladan and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Potparević, Biljana",
year = "2017",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}

Pirković, A., Dekanski, D., Živković, L., Milanović-Čabarkapa, M., Bajić, V., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023

Pirković A, Dekanski D, Živković L, Milanović-Čabarkapa M, Bajić V, Topalović D, Giampieri F, Gasparrini M, Battino M, Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623.
doi:10.1016/j.fct.2016.12.023 .

Pirković, Andrea, Dekanski, Dragana, Živković, Lada, Milanović-Čabarkapa, Mirjana, Bajić, Vladan, Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 . .

TY  - CONF
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5231
AB  - The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
PB  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016
C3  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book
T1  - Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5231
ER  - 

@conference{
author = "Čabarkapa, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.",
publisher = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016",
journal = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book",
title = "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5231"
}

Čabarkapa, A., Živković, L., Dekanski, D., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2016). Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book
International Congress on Occupational & Environmental Toxicology - ICOETox 2016., 135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5231

Čabarkapa A, Živković L, Dekanski D, Topalović D, Bajić V, Spremo-Potparević B. Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book. 2016;:135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5231 .

Čabarkapa, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers" in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book (2016):135-135,
https://hdl.handle.net/21.15107/rcub_farfar_5231 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Zlatković-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jančić, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2584
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright
PB  - Wiley-Blackwell, Hoboken
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Pirković, Andrea and Živković, Lada and Borozan, Sunčica and Zlatković-Svenda, Mirjana and Dekanski, Dragana and Jančić, Ivan and Radak-Perović, Marija and Bajić, Vladan and Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Pirković, A., Živković, L., Borozan, S., Zlatković-Svenda, M., Dekanski, D., Jančić, I., Radak-Perović, M., Bajić, V.,& Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research
Wiley-Blackwell, Hoboken., 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Pirković A, Živković L, Borozan S, Zlatković-Svenda M, Dekanski D, Jančić I, Radak-Perović M, Bajić V, Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Pirković, Andrea, Živković, Lada, Borozan, Sunčica, Zlatković-Svenda, Mirjana, Dekanski, Dragana, Jančić, Ivan, Radak-Perović, Marija, Bajić, Vladan, Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2384
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Pirković, Andrea and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2015",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}

Topalović, D., Živković, L., Pirković, A., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2015/762192

Topalović D, Živković L, Pirković A, Đelić N, Bajić V, Dekanski D, Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity. 2015;.
doi:10.1155/2015/762192 .

Topalović, Dijana, Živković, Lada, Pirković, Andrea, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" in Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 . .

TY  - CONF
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Žukovec-Topalović, Dijana
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Borozan, Sunčica
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5131
AB  - Increased oxidative stress caused by chronic inflammation is one of the underlying mechanisms in etiology of rheumatoid arthritis (RA). Reactive oxygen species produced during inflammatory response target cells DNA leading to important alterations. Novel therapeutic approaches in RA tend to combine the use of standard medications such as methotrexate with natural biologicaly active compounds, aimed to achive better antiinflamatory effect and improvement in clinical parameters, at the same time reducing medication-related side effects. Dry olive leaf extract (DOLE) is known for its antioxidant and anti-inflammatory properties, making it prospective for supplementation in RA. The aim of this study was to evaluate the ability of DOLE to protect against oxidative DNA damage in peripheral blood lymphocytes of chronic RA patients, by use of the comet assay. ...
PB  - Center for Molecular Medicine Cologne, University of Cologne
C3  - 30th Ernst Klenk Symposium  in Molecular Medicine - DNA Damage Response and Repair Mechanisms in Aging and Disease, Sept. 21 – 23, 2014, Abstract Book
T1  - Dry olive leaf extract supplementation reduces DNA damage in chronic rheumatoid arthritis patients
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5131
ER  - 

@conference{
author = "Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Žukovec-Topalović, Dijana and Bajić, Vladan and Dekanski, Dragana and Borozan, Sunčica and Spremo-Potparević, Biljana",
year = "2014",
abstract = "Increased oxidative stress caused by chronic inflammation is one of the underlying mechanisms in etiology of rheumatoid arthritis (RA). Reactive oxygen species produced during inflammatory response target cells DNA leading to important alterations. Novel therapeutic approaches in RA tend to combine the use of standard medications such as methotrexate with natural biologicaly active compounds, aimed to achive better antiinflamatory effect and improvement in clinical parameters, at the same time reducing medication-related side effects. Dry olive leaf extract (DOLE) is known for its antioxidant and anti-inflammatory properties, making it prospective for supplementation in RA. The aim of this study was to evaluate the ability of DOLE to protect against oxidative DNA damage in peripheral blood lymphocytes of chronic RA patients, by use of the comet assay. ...",
publisher = "Center for Molecular Medicine Cologne, University of Cologne",
journal = "30th Ernst Klenk Symposium  in Molecular Medicine - DNA Damage Response and Repair Mechanisms in Aging and Disease, Sept. 21 – 23, 2014, Abstract Book",
title = "Dry olive leaf extract supplementation reduces DNA damage in chronic rheumatoid arthritis patients",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5131"
}

Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Žukovec-Topalović, D., Bajić, V., Dekanski, D., Borozan, S.,& Spremo-Potparević, B.. (2014). Dry olive leaf extract supplementation reduces DNA damage in chronic rheumatoid arthritis patients. in 30th Ernst Klenk Symposium  in Molecular Medicine - DNA Damage Response and Repair Mechanisms in Aging and Disease, Sept. 21 – 23, 2014, Abstract Book
Center for Molecular Medicine Cologne, University of Cologne., 19-19.
https://hdl.handle.net/21.15107/rcub_farfar_5131

Čabarkapa A, Živković L, Zlatković-Švenda M, Žukovec-Topalović D, Bajić V, Dekanski D, Borozan S, Spremo-Potparević B. Dry olive leaf extract supplementation reduces DNA damage in chronic rheumatoid arthritis patients. in 30th Ernst Klenk Symposium  in Molecular Medicine - DNA Damage Response and Repair Mechanisms in Aging and Disease, Sept. 21 – 23, 2014, Abstract Book. 2014;:19-19.
https://hdl.handle.net/21.15107/rcub_farfar_5131 .

Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Žukovec-Topalović, Dijana, Bajić, Vladan, Dekanski, Dragana, Borozan, Sunčica, Spremo-Potparević, Biljana, "Dry olive leaf extract supplementation reduces DNA damage in chronic rheumatoid arthritis patients" in 30th Ernst Klenk Symposium  in Molecular Medicine - DNA Damage Response and Repair Mechanisms in Aging and Disease, Sept. 21 – 23, 2014, Abstract Book (2014):19-19,
https://hdl.handle.net/21.15107/rcub_farfar_5131 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2156
AB  - Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P  lt  0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P  lt  0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology Letters
T1  - Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes
VL  - 28
IS  - 3
SP  - 451
EP  - 456
DO  - 10.1016/j.tiv.2013.12.014
ER  - 

@article{
author = "Pirković, Andrea and Živković, Lada and Topalović, Dijana and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2014",
abstract = "Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P  lt  0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P  lt  0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes",
volume = "28",
number = "3",
pages = "451-456",
doi = "10.1016/j.tiv.2013.12.014"
}

Pirković, A., Živković, L., Topalović, D., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2014). Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 28(3), 451-456.
https://doi.org/10.1016/j.tiv.2013.12.014

Pirković A, Živković L, Topalović D, Đelić N, Bajić V, Dekanski D, Potparević B. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters. 2014;28(3):451-456.
doi:10.1016/j.tiv.2013.12.014 .

Pirković, Andrea, Živković, Lada, Topalović, Dijana, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes" in Toxicology Letters, 28, no. 3 (2014):451-456,
https://doi.org/10.1016/j.tiv.2013.12.014 . .

TY  - CONF
AU  - Topalović, Dijana
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1876
PB  - Karger, Basel
C3  - Annals of Nutrition and Metabolism
T1  - Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin
VL  - 62
IS  - Supplement 2
SP  - 51
EP  - 51
DO  - 10.1159/000351281
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_985
ER  - 

@conference{
author = "Topalović, Dijana and Čabarkapa, Andrea and Živković, Lada and Đelić, Ninoslav and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2013",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin",
volume = "62",
number = "Supplement 2",
pages = "51-51",
doi = "10.1159/000351281",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_985"
}

Topalović, D., Čabarkapa, A., Živković, L., Đelić, N., Dekanski, D., Bajić, V.,& Potparević, B.. (2013). Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin. in Annals of Nutrition and Metabolism
Karger, Basel., 62(Supplement 2), 51-51.
https://doi.org/10.1159/000351281
https://hdl.handle.net/21.15107/rcub_veterinar_985

Topalović D, Čabarkapa A, Živković L, Đelić N, Dekanski D, Bajić V, Potparević B. Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin. in Annals of Nutrition and Metabolism. 2013;62(Supplement 2):51-51.
doi:10.1159/000351281
https://hdl.handle.net/21.15107/rcub_veterinar_985 .

Topalović, Dijana, Čabarkapa, Andrea, Živković, Lada, Đelić, Ninoslav, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin" in Annals of Nutrition and Metabolism, 62, no. Supplement 2 (2013):51-51,
https://doi.org/10.1159/000351281 .,
https://hdl.handle.net/21.15107/rcub_veterinar_985 .