TY  - JOUR
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Starčević, Marija
AU  - Samardžić, Stevan
AU  - Veličković, Ivona
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5551
AB  - The present study aimed to evaluate the antioxidant capacity of ethanolic extracts from post-harvest sunflower and maize stalk residues, and their impact on the chemical composition, physicochemical parameters, lipid oxidative stability, microbiological properties, and sensory characteristics of pork liver pâtés over a 90-day storage period. Four formulations were prepared: a control group (CON), a batch with butylated hydroxytoluene as a synthetic antioxidant (BHT), 1% ethanolic extract from sunflower residues (SSRE), and 1% ethanolic extract from maize residues (MSRE). The MSRE had a higher total phenol content and showed better antioxidant activity relative to the SSRE (p < 0.01). The addition of SSRE decreased the lightness and increased the redness in the pork liver pâtés, with these pâtés showing the highest total color difference compared to the control (p < 0.01). The crop extracts increased the n-6 and total PUFA contents in pâtés and improved the PUFA/SFA ratio (p < 0.01). Formulations containing crop residue extracts showed higher TBARs and POV values than the control and BHT group (p < 0.01), indicating a pro-oxidant effect and accelerated lipid oxidation in pâtés during storage. As far as microbiological quality, the presence of crop residue extracts decreased the total viable count, lactic acid bacteria, and psychotropic aerobic bacteria (p < 0.01). The incorporation of crop extracts in the pork pâtés impaired their sensory quality, particularly color, odor, aroma, and flavor, and decreased their overall acceptability. These results indicated that, while the crop residue extracts were not as effective as synthetic antioxidants in preserving the lipid stability of pâtés, they demonstrated potential for enhancing the microbial quality of this type of meat product.
PB  - MDPI
T2  - Foods
T1  - The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés
VL  - 13
IS  - 5
SP  - 788
DO  - 10.3390/foods13050788
ER  - 

@article{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Starčević, Marija and Samardžić, Stevan and Veličković, Ivona and Maksimović, Zoran",
year = "2024",
abstract = "The present study aimed to evaluate the antioxidant capacity of ethanolic extracts from post-harvest sunflower and maize stalk residues, and their impact on the chemical composition, physicochemical parameters, lipid oxidative stability, microbiological properties, and sensory characteristics of pork liver pâtés over a 90-day storage period. Four formulations were prepared: a control group (CON), a batch with butylated hydroxytoluene as a synthetic antioxidant (BHT), 1% ethanolic extract from sunflower residues (SSRE), and 1% ethanolic extract from maize residues (MSRE). The MSRE had a higher total phenol content and showed better antioxidant activity relative to the SSRE (p < 0.01). The addition of SSRE decreased the lightness and increased the redness in the pork liver pâtés, with these pâtés showing the highest total color difference compared to the control (p < 0.01). The crop extracts increased the n-6 and total PUFA contents in pâtés and improved the PUFA/SFA ratio (p < 0.01). Formulations containing crop residue extracts showed higher TBARs and POV values than the control and BHT group (p < 0.01), indicating a pro-oxidant effect and accelerated lipid oxidation in pâtés during storage. As far as microbiological quality, the presence of crop residue extracts decreased the total viable count, lactic acid bacteria, and psychotropic aerobic bacteria (p < 0.01). The incorporation of crop extracts in the pork pâtés impaired their sensory quality, particularly color, odor, aroma, and flavor, and decreased their overall acceptability. These results indicated that, while the crop residue extracts were not as effective as synthetic antioxidants in preserving the lipid stability of pâtés, they demonstrated potential for enhancing the microbial quality of this type of meat product.",
publisher = "MDPI",
journal = "Foods",
title = "The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés",
volume = "13",
number = "5",
pages = "788",
doi = "10.3390/foods13050788"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Starčević, M., Samardžić, S., Veličković, I.,& Maksimović, Z.. (2024). The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés. in Foods
MDPI., 13(5), 788.
https://doi.org/10.3390/foods13050788

Glišić M, Bošković Cabrol M, Čobanović N, Starčević M, Samardžić S, Veličković I, Maksimović Z. The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés. in Foods. 2024;13(5):788.
doi:10.3390/foods13050788 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Starčević, Marija, Samardžić, Stevan, Veličković, Ivona, Maksimović, Zoran, "The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés" in Foods, 13, no. 5 (2024):788,
https://doi.org/10.3390/foods13050788 . .

TY  - CONF
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Samardžić, Stevan
AU  - Baltić, Milan Ž.
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5198
AB  - Modern agriculture produces a large amount of residue every year causing environmental pollution
due to limited and inadequate agricultural waste management. Previous research has shown that agro-industrial residues are important sources of various bioactive, mainly phenolic, compounds that could be applied in the meat industry as natural antimicrobials and antioxidants. This study aimed to assess the effect of the addition of sunflower and maize crop residue extracts on physicochemical (pH and colour) characteristics and microbiological quality of pork liver pâtés over three months of storage and compared to a synthetic antioxidant (butylated hydroxytoluene - BHT) and a control batch. The sunflower and maize crop ethanolic extracts improved the microbial quality of the pork liver pâtés indicating the potential of these extracts as natural preservatives in meat products. However, the addition of 1% of crop extracts resulted in a darker product with a higher b* value, especially after 3 months of storage.
C3  - 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy
T1  - Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté
SP  - 579
EP  - 580
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5198
ER  - 

@conference{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Samardžić, Stevan and Baltić, Milan Ž. and Maksimović, Zoran",
year = "2023",
abstract = "Modern agriculture produces a large amount of residue every year causing environmental pollution
due to limited and inadequate agricultural waste management. Previous research has shown that agro-industrial residues are important sources of various bioactive, mainly phenolic, compounds that could be applied in the meat industry as natural antimicrobials and antioxidants. This study aimed to assess the effect of the addition of sunflower and maize crop residue extracts on physicochemical (pH and colour) characteristics and microbiological quality of pork liver pâtés over three months of storage and compared to a synthetic antioxidant (butylated hydroxytoluene - BHT) and a control batch. The sunflower and maize crop ethanolic extracts improved the microbial quality of the pork liver pâtés indicating the potential of these extracts as natural preservatives in meat products. However, the addition of 1% of crop extracts resulted in a darker product with a higher b* value, especially after 3 months of storage.",
journal = "69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy",
title = "Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté",
pages = "579-580",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5198"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Samardžić, S., Baltić, M. Ž.,& Maksimović, Z.. (2023). Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté. in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy, 579-580.
https://hdl.handle.net/21.15107/rcub_farfar_5198

Glišić M, Bošković Cabrol M, Čobanović N, Samardžić S, Baltić MŽ, Maksimović Z. Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté. in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy. 2023;:579-580.
https://hdl.handle.net/21.15107/rcub_farfar_5198 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Samardžić, Stevan, Baltić, Milan Ž., Maksimović, Zoran, "Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté" in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy (2023):579-580,
https://hdl.handle.net/21.15107/rcub_farfar_5198 .

TY  - JOUR
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Baltić, Milan Ž.
AU  - Vranešević, Jelena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4954
AB  - Large quantities of agricultural residues are generated every year. Most of the crop-based residues are underutilized, mainly left to decay on the land or to be burnt, which can lead to an increase in a load of environmental pollution. Considering this, different strategies have been developed to use these renewable resources as raw materials for the production of bioactive compounds, their isolation and characterization, and potential application in a wide range of fields, particularly in the food industry as natural preservatives. In this study, the antibacterial efficacy of wheat, sunflower, and maize crop residue ethanolic extracts against six bacterial strains (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Yersinia enterocolitica) was evaluated by the broth microdilution method. Used extracts inhibited the growth of selected microorganisms with a minimal inhibitory concentration (MIC) of 320 μg/mL for most of the tested bacteria. L. monocytogenes showed a MIC value of 640 μg/mL for wheat ethanolic extract, and the MIC value of sunflower ethanolic extract for S. Typhimurium was 160 μg/mL. There were no minimum bactericidal concentration (MBC) values for any of the bacteria within the extract’s concentration ranges tested (≤ 2560 μg/mL). The results of the present study indicate that crop residue ethanolic extracts could exhibit bacteriostatic effect and therefore have the potential as natural additives in food preservation.
AB  - Velika količina poljoprivrednih ostataka se proizvodi svake godine. Većina žetvenih ostataka je nedovoljno iskorišćena, uglavnom se ostavlja da propadne na njivama ili se spaljuje, što dovodi do povećanog zagađenja životne sredine. Imajući ovo u vidu, razvijene su različite strategije za iskorišćavanje navedenih obnovljivih resursa kao sirovina u proizvodnji bioaktivnih jedinjenja, njihovu izolaciju i karakterizaciju i potencijalnu primenu u različitim oblastima, naročito u industriji hrane kao prirodni konzervansi. U ovoj studiji ispitivana je antibakterijska efikasnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza na šest bakterijskih sojeva (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes i Yersinia enterocolitica) primenom mikrodilucione metode u bujonu. Ekstrakti žetvenih ostataka su inhibirali rast odabranih mikroorganizama u minimalnoj inhibitornoj koncentraciji (MIC) od 320 μg/mL za većinu ispitivanih bakterija. Za L. monocytogenes MIC vrednost etanolnog ekstrakta pšenice bila je 640 μg/ mL, a 160 μg/mL je bila MIC vrednost etanolnog ekstrakta suncokreta za S. Typhimurium. Minimalna baktericidna koncentracija (MBC) prema bakterijama nije postignuta u ispitivanim koncentracijama etanolnih ekstrakata (≤ 2560 μg/mL). Rezultati ukazuju da etanolni ekstrakti žetvenih rezidua bi mogli da ostvare antibakterijski efekat i stoga imaju potencijal kao prirodni aditivi u konzervisanju hrane.
PB  - Scientific Veterinary Institute "Novi Sad"
T2  - Archives of Veterinary Medicine
T1  - Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues
T1  - Antibakterijska aktivnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza
VL  - 16
IS  - 1
SP  - 53
EP  - 67
DO  - 10.46784/e-avm.v16i1.315
ER  - 

@article{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Baltić, Milan Ž. and Vranešević, Jelena and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Large quantities of agricultural residues are generated every year. Most of the crop-based residues are underutilized, mainly left to decay on the land or to be burnt, which can lead to an increase in a load of environmental pollution. Considering this, different strategies have been developed to use these renewable resources as raw materials for the production of bioactive compounds, their isolation and characterization, and potential application in a wide range of fields, particularly in the food industry as natural preservatives. In this study, the antibacterial efficacy of wheat, sunflower, and maize crop residue ethanolic extracts against six bacterial strains (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Yersinia enterocolitica) was evaluated by the broth microdilution method. Used extracts inhibited the growth of selected microorganisms with a minimal inhibitory concentration (MIC) of 320 μg/mL for most of the tested bacteria. L. monocytogenes showed a MIC value of 640 μg/mL for wheat ethanolic extract, and the MIC value of sunflower ethanolic extract for S. Typhimurium was 160 μg/mL. There were no minimum bactericidal concentration (MBC) values for any of the bacteria within the extract’s concentration ranges tested (≤ 2560 μg/mL). The results of the present study indicate that crop residue ethanolic extracts could exhibit bacteriostatic effect and therefore have the potential as natural additives in food preservation., Velika količina poljoprivrednih ostataka se proizvodi svake godine. Većina žetvenih ostataka je nedovoljno iskorišćena, uglavnom se ostavlja da propadne na njivama ili se spaljuje, što dovodi do povećanog zagađenja životne sredine. Imajući ovo u vidu, razvijene su različite strategije za iskorišćavanje navedenih obnovljivih resursa kao sirovina u proizvodnji bioaktivnih jedinjenja, njihovu izolaciju i karakterizaciju i potencijalnu primenu u različitim oblastima, naročito u industriji hrane kao prirodni konzervansi. U ovoj studiji ispitivana je antibakterijska efikasnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza na šest bakterijskih sojeva (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes i Yersinia enterocolitica) primenom mikrodilucione metode u bujonu. Ekstrakti žetvenih ostataka su inhibirali rast odabranih mikroorganizama u minimalnoj inhibitornoj koncentraciji (MIC) od 320 μg/mL za većinu ispitivanih bakterija. Za L. monocytogenes MIC vrednost etanolnog ekstrakta pšenice bila je 640 μg/ mL, a 160 μg/mL je bila MIC vrednost etanolnog ekstrakta suncokreta za S. Typhimurium. Minimalna baktericidna koncentracija (MBC) prema bakterijama nije postignuta u ispitivanim koncentracijama etanolnih ekstrakata (≤ 2560 μg/mL). Rezultati ukazuju da etanolni ekstrakti žetvenih rezidua bi mogli da ostvare antibakterijski efekat i stoga imaju potencijal kao prirodni aditivi u konzervisanju hrane.",
publisher = "Scientific Veterinary Institute "Novi Sad"",
journal = "Archives of Veterinary Medicine",
title = "Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues, Antibakterijska aktivnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza",
volume = "16",
number = "1",
pages = "53-67",
doi = "10.46784/e-avm.v16i1.315"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Baltić, M. Ž., Vranešević, J., Samardžić, S.,& Maksimović, Z.. (2023). Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues. in Archives of Veterinary Medicine
Scientific Veterinary Institute "Novi Sad"., 16(1), 53-67.
https://doi.org/10.46784/e-avm.v16i1.315

Glišić M, Bošković Cabrol M, Čobanović N, Baltić MŽ, Vranešević J, Samardžić S, Maksimović Z. Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues. in Archives of Veterinary Medicine. 2023;16(1):53-67.
doi:10.46784/e-avm.v16i1.315 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Baltić, Milan Ž., Vranešević, Jelena, Samardžić, Stevan, Maksimović, Zoran, "Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues" in Archives of Veterinary Medicine, 16, no. 1 (2023):53-67,
https://doi.org/10.46784/e-avm.v16i1.315 . .

TY  - CONF
AU  - Samardžić, Stevan
AU  - Veličković, Ivona
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5251
AB  - The sunflower (Helianthus annuus L.), which belongs to the Asteraceae family, is an annual plant native to North America. It is grown on approximately 28 million hectares worldwide, making it the third most important oilseed crop after soybean and rapeseed. ...
PB  - Phytochemical Society of Europe (PSE)
C3  - PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
T1  - Valorization of Sunflower Waste Ethanol Extract and Its Ethyl-Acetate Fraction
SP  - 99
EP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5251
ER  - 

@conference{
author = "Samardžić, Stevan and Veličković, Ivona and Maksimović, Zoran",
year = "2023",
abstract = "The sunflower (Helianthus annuus L.), which belongs to the Asteraceae family, is an annual plant native to North America. It is grown on approximately 28 million hectares worldwide, making it the third most important oilseed crop after soybean and rapeseed. ...",
publisher = "Phytochemical Society of Europe (PSE)",
journal = "PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book",
title = "Valorization of Sunflower Waste Ethanol Extract and Its Ethyl-Acetate Fraction",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5251"
}

Samardžić, S., Veličković, I.,& Maksimović, Z.. (2023). Valorization of Sunflower Waste Ethanol Extract and Its Ethyl-Acetate Fraction. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
Phytochemical Society of Europe (PSE)., 99-99.
https://hdl.handle.net/21.15107/rcub_farfar_5251

Samardžić S, Veličković I, Maksimović Z. Valorization of Sunflower Waste Ethanol Extract and Its Ethyl-Acetate Fraction. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book. 2023;:99-99.
https://hdl.handle.net/21.15107/rcub_farfar_5251 .

Samardžić, Stevan, Veličković, Ivona, Maksimović, Zoran, "Valorization of Sunflower Waste Ethanol Extract and Its Ethyl-Acetate Fraction" in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book (2023):99-99,
https://hdl.handle.net/21.15107/rcub_farfar_5251 .

TY  - CONF
AU  - Samardžić, Stevan
AU  - Veličković, Ivona
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5250
AB  - Wheat (Triticum aestivum L., Poaceae), the most common cereal in the human diet in Europe and the United States, generates an enormous amount of underutilized agricultural byproducts. ...
PB  - Phytochemical Society of Europe (PSE)
C3  - PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
T1  - Chemical Composition, Antioxidant, and Enzyme-Inhibitory Potential of Wheat Harvest Residue
SP  - 98
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5250
ER  - 

@conference{
author = "Samardžić, Stevan and Veličković, Ivona and Maksimović, Zoran",
year = "2023",
abstract = "Wheat (Triticum aestivum L., Poaceae), the most common cereal in the human diet in Europe and the United States, generates an enormous amount of underutilized agricultural byproducts. ...",
publisher = "Phytochemical Society of Europe (PSE)",
journal = "PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book",
title = "Chemical Composition, Antioxidant, and Enzyme-Inhibitory Potential of Wheat Harvest Residue",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5250"
}

Samardžić, S., Veličković, I.,& Maksimović, Z.. (2023). Chemical Composition, Antioxidant, and Enzyme-Inhibitory Potential of Wheat Harvest Residue. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
Phytochemical Society of Europe (PSE)., 98-98.
https://hdl.handle.net/21.15107/rcub_farfar_5250

Samardžić S, Veličković I, Maksimović Z. Chemical Composition, Antioxidant, and Enzyme-Inhibitory Potential of Wheat Harvest Residue. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_farfar_5250 .

Samardžić, Stevan, Veličković, Ivona, Maksimović, Zoran, "Chemical Composition, Antioxidant, and Enzyme-Inhibitory Potential of Wheat Harvest Residue" in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book (2023):98-98,
https://hdl.handle.net/21.15107/rcub_farfar_5250 .

TY  - CONF
AU  - Veličković, Ivona
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5249
AB  - One of the most current problems is ensuring food supply for the growing population, which has led to a tripling of agricultural production in recent decades.  ...
PB  - Phytochemical Society of Europe (PSE)
C3  - PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
T1  - Could Agricultural Waste Material of Wheat, Maize and Sunfower Waste Be A New Source of Bioactive Compounds?
SP  - 97
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5249
ER  - 

@conference{
author = "Veličković, Ivona and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "One of the most current problems is ensuring food supply for the growing population, which has led to a tripling of agricultural production in recent decades.  ...",
publisher = "Phytochemical Society of Europe (PSE)",
journal = "PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book",
title = "Could Agricultural Waste Material of Wheat, Maize and Sunfower Waste Be A New Source of Bioactive Compounds?",
pages = "97-97",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5249"
}

Veličković, I., Samardžić, S.,& Maksimović, Z.. (2023). Could Agricultural Waste Material of Wheat, Maize and Sunfower Waste Be A New Source of Bioactive Compounds?. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
Phytochemical Society of Europe (PSE)., 97-97.
https://hdl.handle.net/21.15107/rcub_farfar_5249

Veličković I, Samardžić S, Maksimović Z. Could Agricultural Waste Material of Wheat, Maize and Sunfower Waste Be A New Source of Bioactive Compounds?. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book. 2023;:97-97.
https://hdl.handle.net/21.15107/rcub_farfar_5249 .

Veličković, Ivona, Samardžić, Stevan, Maksimović, Zoran, "Could Agricultural Waste Material of Wheat, Maize and Sunfower Waste Be A New Source of Bioactive Compounds?" in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book (2023):97-97,
https://hdl.handle.net/21.15107/rcub_farfar_5249 .

TY  - CONF
AU  - Veličković, Ivona
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5248
AB  - Maize (Zea mays L., Poaceae) is one of the most commonly grown cereals after wheat and rice. ...
PB  - Phytochemical Society of Europe (PSE)
C3  - PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
T1  - Phytochemical Composition and In Vitro Bioactivity of Maize Harvest Residue
SP  - 96
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5248
ER  - 

@conference{
author = "Veličković, Ivona and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Maize (Zea mays L., Poaceae) is one of the most commonly grown cereals after wheat and rice. ...",
publisher = "Phytochemical Society of Europe (PSE)",
journal = "PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book",
title = "Phytochemical Composition and In Vitro Bioactivity of Maize Harvest Residue",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5248"
}

Veličković, I., Samardžić, S.,& Maksimović, Z.. (2023). Phytochemical Composition and In Vitro Bioactivity of Maize Harvest Residue. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book
Phytochemical Society of Europe (PSE)., 96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5248

Veličković I, Samardžić S, Maksimović Z. Phytochemical Composition and In Vitro Bioactivity of Maize Harvest Residue. in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5248 .

Veličković, Ivona, Samardžić, Stevan, Maksimović, Zoran, "Phytochemical Composition and In Vitro Bioactivity of Maize Harvest Residue" in PSE Natural Drug Discovery Current Approach and Future Perspectives. Abstract Book (2023):96-96,
https://hdl.handle.net/21.15107/rcub_farfar_5248 .

TY  - JOUR
AU  - Mićović, Tijana
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5463
AB  - In this study, antimicrobial activity of essential oils extracted from the aerial flowering parts (herbs) of Hyssopus officinalis subsp. aristatus (Godr.) Nyman (Lamiaceae) collected from five different locations in Montenegro, or purchased in Serbia, were investigated. In addition, their antibacterial activity in combination with antibiotics was studied. The antimicrobial activity against selected standard bacterial and yeast strains was investigated using the broth microdilution method. Two standard antibiotics were used for comparison: the aminoglycoside antibiotic amikacin and the cephalosporin antibiotic ceftriaxone. The antimicrobial activity of the essential oil-amikacin combination was investigated using the checkerboard assay. The main components of the essential oils were 1,8-cineole, cis-pinocamphone, β-pinene and limonene in varying quantities. Most of the tested essential oils showed no significant antimicrobial activity. However, an essential oil rich in cis-pinocamphone showed moderate activity against both Staphylococcus aureus and Escherichia coli (MIC = 400 µg/mL). The overall effect of the essential oils and antibiotic combinations against E. coli or S. aureus ranged from additive (FICI = 0.625) to indifferent (FICI = 1.5), depending on the source of the essential oil.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Antimicrobial activity of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae) essential oils from Montenegro and Serbia
VL  - 43
DO  - 10.61652/leksir2343e173M
ER  - 

@article{
author = "Mićović, Tijana and Ušjak, Dušan and Milenković, Marina and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "In this study, antimicrobial activity of essential oils extracted from the aerial flowering parts (herbs) of Hyssopus officinalis subsp. aristatus (Godr.) Nyman (Lamiaceae) collected from five different locations in Montenegro, or purchased in Serbia, were investigated. In addition, their antibacterial activity in combination with antibiotics was studied. The antimicrobial activity against selected standard bacterial and yeast strains was investigated using the broth microdilution method. Two standard antibiotics were used for comparison: the aminoglycoside antibiotic amikacin and the cephalosporin antibiotic ceftriaxone. The antimicrobial activity of the essential oil-amikacin combination was investigated using the checkerboard assay. The main components of the essential oils were 1,8-cineole, cis-pinocamphone, β-pinene and limonene in varying quantities. Most of the tested essential oils showed no significant antimicrobial activity. However, an essential oil rich in cis-pinocamphone showed moderate activity against both Staphylococcus aureus and Escherichia coli (MIC = 400 µg/mL). The overall effect of the essential oils and antibiotic combinations against E. coli or S. aureus ranged from additive (FICI = 0.625) to indifferent (FICI = 1.5), depending on the source of the essential oil.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Antimicrobial activity of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae) essential oils from Montenegro and Serbia",
volume = "43",
doi = "10.61652/leksir2343e173M"
}

Mićović, T., Ušjak, D., Milenković, M., Samardžić, S.,& Maksimović, Z.. (2023). Antimicrobial activity of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae) essential oils from Montenegro and Serbia. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd., 43.
https://doi.org/10.61652/leksir2343e173M

Mićović T, Ušjak D, Milenković M, Samardžić S, Maksimović Z. Antimicrobial activity of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae) essential oils from Montenegro and Serbia. in Lekovite sirovine. 2023;43.
doi:10.61652/leksir2343e173M .

Mićović, Tijana, Ušjak, Dušan, Milenković, Marina, Samardžić, Stevan, Maksimović, Zoran, "Antimicrobial activity of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae) essential oils from Montenegro and Serbia" in Lekovite sirovine, 43 (2023),
https://doi.org/10.61652/leksir2343e173M . .

TY  - CONF
AU  - Romanić, Ranko
AU  - Lužaić, Tanja
AU  - Kravić, Snežana
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5400
AB  - Poljoprivredni otpad koji nastaje nakon biljne proizvodnje je još uvek u velikoj meri nedovoljno iskorišćen. Često se spaljuje na poljima, posebno u zemljama u razvoju koje nemaju jake regulatorne instrumente za kontrolu takve prakse, uključujući Srbiju. Kao uobičajena praksa, otvoreno sagorevanje poljoprivrednih ostataka dovodi do zagađenja vazduha, što predstavlja rizik po zdravlje ljudi i po životnu sredinu. Pored toga, dovodi do potpunog gubitka organske materije i azota, ugrožava divljač i predstavlja veliku opasnost zbog mogućnosti nekontrolisanog širenja požara. Primena žetvenih ostataka pšenice, kukuruza i suncokreta još uvek nije dovoljno istražena, nema visokovrednu primenu, pa je cilj ovog rada da se ispita sastav heksanskih ekstrakata žetvenih ostataka pšenice, kukuruza i suncokreta sa posebnim akcentom na ispitivanje sastava masnih kiselina. Utvrđeno je da ne postoji značajna različitost među ispitanim uzorcima u sadržaju linolne masne kiseline. Sadržaj oleinske masne kiseline je značajno niži u lipidnom ekstraktu žetvenih ostataka pšenice, u poređenju sa kukuruzom i suncokretom. Sa druge strane, sadržaj zasićenih masnih kiselina, palmitinske i lignocerinske masne kiseline, dobijen u ekstraktu žetvenih ostataka pšenice je značajno veći. U sadržaju ostalih masnih kiselina nije utvrđena značajna razlika među ispitanim uzorcima.
PB  - Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad
PB  - Institut za ratarstvo i povrtarstvo Novi Sad
PB  - "Industrijsko bilje" Doo Novi Sad
C3  - Zbornik radova - 64. savetovanje "Proizvodnja i prerada uljarica" sa međunarodnim učešćem
T1  - Žetveni ostaci pšenice, kukuruza i suncokreta – Sastav lipidnih ekstrakata
SP  - 19
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5400
ER  - 

@conference{
author = "Romanić, Ranko and Lužaić, Tanja and Kravić, Snežana and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Poljoprivredni otpad koji nastaje nakon biljne proizvodnje je još uvek u velikoj meri nedovoljno iskorišćen. Često se spaljuje na poljima, posebno u zemljama u razvoju koje nemaju jake regulatorne instrumente za kontrolu takve prakse, uključujući Srbiju. Kao uobičajena praksa, otvoreno sagorevanje poljoprivrednih ostataka dovodi do zagađenja vazduha, što predstavlja rizik po zdravlje ljudi i po životnu sredinu. Pored toga, dovodi do potpunog gubitka organske materije i azota, ugrožava divljač i predstavlja veliku opasnost zbog mogućnosti nekontrolisanog širenja požara. Primena žetvenih ostataka pšenice, kukuruza i suncokreta još uvek nije dovoljno istražena, nema visokovrednu primenu, pa je cilj ovog rada da se ispita sastav heksanskih ekstrakata žetvenih ostataka pšenice, kukuruza i suncokreta sa posebnim akcentom na ispitivanje sastava masnih kiselina. Utvrđeno je da ne postoji značajna različitost među ispitanim uzorcima u sadržaju linolne masne kiseline. Sadržaj oleinske masne kiseline je značajno niži u lipidnom ekstraktu žetvenih ostataka pšenice, u poređenju sa kukuruzom i suncokretom. Sa druge strane, sadržaj zasićenih masnih kiselina, palmitinske i lignocerinske masne kiseline, dobijen u ekstraktu žetvenih ostataka pšenice je značajno veći. U sadržaju ostalih masnih kiselina nije utvrđena značajna razlika među ispitanim uzorcima.",
publisher = "Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, Institut za ratarstvo i povrtarstvo Novi Sad, "Industrijsko bilje" Doo Novi Sad",
journal = "Zbornik radova - 64. savetovanje "Proizvodnja i prerada uljarica" sa međunarodnim učešćem",
title = "Žetveni ostaci pšenice, kukuruza i suncokreta – Sastav lipidnih ekstrakata",
pages = "19-28",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5400"
}

Romanić, R., Lužaić, T., Kravić, S., Samardžić, S.,& Maksimović, Z.. (2023). Žetveni ostaci pšenice, kukuruza i suncokreta – Sastav lipidnih ekstrakata. in Zbornik radova - 64. savetovanje "Proizvodnja i prerada uljarica" sa međunarodnim učešćem
Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad., 19-28.
https://hdl.handle.net/21.15107/rcub_farfar_5400

Romanić R, Lužaić T, Kravić S, Samardžić S, Maksimović Z. Žetveni ostaci pšenice, kukuruza i suncokreta – Sastav lipidnih ekstrakata. in Zbornik radova - 64. savetovanje "Proizvodnja i prerada uljarica" sa međunarodnim učešćem. 2023;:19-28.
https://hdl.handle.net/21.15107/rcub_farfar_5400 .

Romanić, Ranko, Lužaić, Tanja, Kravić, Snežana, Samardžić, Stevan, Maksimović, Zoran, "Žetveni ostaci pšenice, kukuruza i suncokreta – Sastav lipidnih ekstrakata" in Zbornik radova - 64. savetovanje "Proizvodnja i prerada uljarica" sa međunarodnim učešćem (2023):19-28,
https://hdl.handle.net/21.15107/rcub_farfar_5400 .

TY  - CONF
AU  - Romanić, Ranko
AU  - Lužaić, Tanja
AU  - Kravić, Snežana
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5200
AB  - In Serbia, agricultural waste is insufficiently used waste, because it is most often burned. Burning crop residues represents a great environmental risk, because it is a frequent cause of fires. On the other hand, harvest residues contain various components that could be used in the food and pharmaceutical industry. In this paper gas chromatography-mass spectrometry (GC-MS) was utilized to provide comprehensive characterization of the chemical composition of lipid extracts of wheat, corn and sunflower harvest residues.
PB  - Polish Food Technologists Society
C3  - 19th EuroFed Lipid Congress and Expo: Fats, Oils and Lipids - From Raw Materials to Consumer Expectations. Book of Abstracts
T1  - Lipid Extracts of Harvest Residues of Wheat, Corn and Sunflower from Serbia: Investigation of Fatty Acid Composition
SP  - 241
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5200
ER  - 

@conference{
author = "Romanić, Ranko and Lužaić, Tanja and Kravić, Snežana and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "In Serbia, agricultural waste is insufficiently used waste, because it is most often burned. Burning crop residues represents a great environmental risk, because it is a frequent cause of fires. On the other hand, harvest residues contain various components that could be used in the food and pharmaceutical industry. In this paper gas chromatography-mass spectrometry (GC-MS) was utilized to provide comprehensive characterization of the chemical composition of lipid extracts of wheat, corn and sunflower harvest residues.",
publisher = "Polish Food Technologists Society",
journal = "19th EuroFed Lipid Congress and Expo: Fats, Oils and Lipids - From Raw Materials to Consumer Expectations. Book of Abstracts",
title = "Lipid Extracts of Harvest Residues of Wheat, Corn and Sunflower from Serbia: Investigation of Fatty Acid Composition",
pages = "241-241",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5200"
}

Romanić, R., Lužaić, T., Kravić, S., Samardžić, S.,& Maksimović, Z.. (2023). Lipid Extracts of Harvest Residues of Wheat, Corn and Sunflower from Serbia: Investigation of Fatty Acid Composition. in 19th EuroFed Lipid Congress and Expo: Fats, Oils and Lipids - From Raw Materials to Consumer Expectations. Book of Abstracts
Polish Food Technologists Society., 241-241.
https://hdl.handle.net/21.15107/rcub_farfar_5200

Romanić R, Lužaić T, Kravić S, Samardžić S, Maksimović Z. Lipid Extracts of Harvest Residues of Wheat, Corn and Sunflower from Serbia: Investigation of Fatty Acid Composition. in 19th EuroFed Lipid Congress and Expo: Fats, Oils and Lipids - From Raw Materials to Consumer Expectations. Book of Abstracts. 2023;:241-241.
https://hdl.handle.net/21.15107/rcub_farfar_5200 .

Romanić, Ranko, Lužaić, Tanja, Kravić, Snežana, Samardžić, Stevan, Maksimović, Zoran, "Lipid Extracts of Harvest Residues of Wheat, Corn and Sunflower from Serbia: Investigation of Fatty Acid Composition" in 19th EuroFed Lipid Congress and Expo: Fats, Oils and Lipids - From Raw Materials to Consumer Expectations. Book of Abstracts (2023):241-241,
https://hdl.handle.net/21.15107/rcub_farfar_5200 .

TY  - CONF
AU  - Glišić, Milica
AU  - Bošković-Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Baltić, Milan Ž.
AU  - Drašković, Vladimir
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5199
AB  - Globalization and population growth have led to the development of a modern agricultural system that currently produces millions of tons of waste. This waste is disposed of by burning, dumping or accumulating in landfills, resulting in environmental, health, and economic issues.The agro‑industrial residues are abundant with phenolic bioactive compounds, such as phenolic acids, flavonoids, tannins, and carotenoids, which, among others, exhibit antioxidant and antimicrobial capacities and have good potential as food flavorings and colorants. The most common method for isolating these compounds is solvent extraction. However, there is a trend towards eco‑innovative extraction methods that offer better possibilities for implementation on an industrial scale. The oxidation of lipids and proteins is one of the main causes of quality deterioration in meat and meat products during processing and storage. Therefore, the application of natural antioxidants extracted from these new, unconventional raw materials could be a sustainable alternative to synthetic antioxidants. This review summarizes the data on natural antioxidants derived from agro‑industrial by‑products and their incorporation in various meat product formulations. It also addresses limiting factors related to safety and changes in sensory properties.
PB  - Institute of Meat Hygiene and Technology
C3  - Meat Technology - Special Issue
T1  - Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products
VL  - 64
IS  - 2
SP  - 116
EP  - 121
DO  - 10.18485/meattech.2023.64.2.20
ER  - 

@conference{
author = "Glišić, Milica and Bošković-Cabrol, Marija and Čobanović, Nikola and Baltić, Milan Ž. and Drašković, Vladimir and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Globalization and population growth have led to the development of a modern agricultural system that currently produces millions of tons of waste. This waste is disposed of by burning, dumping or accumulating in landfills, resulting in environmental, health, and economic issues.The agro‑industrial residues are abundant with phenolic bioactive compounds, such as phenolic acids, flavonoids, tannins, and carotenoids, which, among others, exhibit antioxidant and antimicrobial capacities and have good potential as food flavorings and colorants. The most common method for isolating these compounds is solvent extraction. However, there is a trend towards eco‑innovative extraction methods that offer better possibilities for implementation on an industrial scale. The oxidation of lipids and proteins is one of the main causes of quality deterioration in meat and meat products during processing and storage. Therefore, the application of natural antioxidants extracted from these new, unconventional raw materials could be a sustainable alternative to synthetic antioxidants. This review summarizes the data on natural antioxidants derived from agro‑industrial by‑products and their incorporation in various meat product formulations. It also addresses limiting factors related to safety and changes in sensory properties.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology - Special Issue",
title = "Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products",
volume = "64",
number = "2",
pages = "116-121",
doi = "10.18485/meattech.2023.64.2.20"
}

Glišić, M., Bošković-Cabrol, M., Čobanović, N., Baltić, M. Ž., Drašković, V., Samardžić, S.,& Maksimović, Z.. (2023). Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products. in Meat Technology - Special Issue
Institute of Meat Hygiene and Technology., 64(2), 116-121.
https://doi.org/10.18485/meattech.2023.64.2.20

Glišić M, Bošković-Cabrol M, Čobanović N, Baltić MŽ, Drašković V, Samardžić S, Maksimović Z. Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products. in Meat Technology - Special Issue. 2023;64(2):116-121.
doi:10.18485/meattech.2023.64.2.20 .

Glišić, Milica, Bošković-Cabrol, Marija, Čobanović, Nikola, Baltić, Milan Ž., Drašković, Vladimir, Samardžić, Stevan, Maksimović, Zoran, "Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products" in Meat Technology - Special Issue, 64, no. 2 (2023):116-121,
https://doi.org/10.18485/meattech.2023.64.2.20 . .

TY  - JOUR
AU  - Kostić, Kristina
AU  - Brborić, Jasmina
AU  - Delogu, Giovanna
AU  - Simić, Milena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
AU  - Dettori, Maria Antonietta
AU  - Fabbri, Davide
AU  - Kotur-Stevuljević, Jelena
AU  - Saso, Luciano
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4648
AB  - A comparative in vitro study of the antioxidant potential of natural phenols (zingerone, curcumin, raspberry ketone, magnolol) and their synthesized derivatives was performed. The antioxidant efficiency was evaluated in blood serum obtained from healthy individuals, by means of spectrophotometry, before and after the addition of pro-oxidant tert-butyl hydroperoxide (TBH). Moreover, the antioxidant effect of an equimolar mixture of curcumin and zingerone was investigated. Interpretation of our results reveals that in the blood serum of healthy individuals curcumin (C1), raspberry ketone (RK1), magnolol (M1) and synthesized derivative of zingerone (Z2) demonstrate remarkable antioxidant effects (p < 0.05). However, in the state of TBH-induced excessive oxidative stress natural magnolol and synthesized derivatives C1, Z1 and RK1 show powerful antioxidant activity and thus can be further investigated to obtain information about their metabolic transformations and their potential influence at the cellular level. Results obtained from measurements in an equimolar mixture of zingerone and curcumin indicate synergism (p < 0.05) between the two compounds. This combination is especially successful due to the fast and efficient neutralization of added pro-oxidant TBH. The commercial availability of turmeric and ginger and their frequent combined use in diet suggest ideas for further broader utilization of the beneficial synergistic effect of their phenolic components.
PB  - MDPI
T2  - Molecules
T1  - Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls
VL  - 28
IS  - 6
DO  - 10.3390/molecules28062646
ER  - 

@article{
author = "Kostić, Kristina and Brborić, Jasmina and Delogu, Giovanna and Simić, Milena and Samardžić, Stevan and Maksimović, Zoran and Dettori, Maria Antonietta and Fabbri, Davide and Kotur-Stevuljević, Jelena and Saso, Luciano",
year = "2023",
abstract = "A comparative in vitro study of the antioxidant potential of natural phenols (zingerone, curcumin, raspberry ketone, magnolol) and their synthesized derivatives was performed. The antioxidant efficiency was evaluated in blood serum obtained from healthy individuals, by means of spectrophotometry, before and after the addition of pro-oxidant tert-butyl hydroperoxide (TBH). Moreover, the antioxidant effect of an equimolar mixture of curcumin and zingerone was investigated. Interpretation of our results reveals that in the blood serum of healthy individuals curcumin (C1), raspberry ketone (RK1), magnolol (M1) and synthesized derivative of zingerone (Z2) demonstrate remarkable antioxidant effects (p < 0.05). However, in the state of TBH-induced excessive oxidative stress natural magnolol and synthesized derivatives C1, Z1 and RK1 show powerful antioxidant activity and thus can be further investigated to obtain information about their metabolic transformations and their potential influence at the cellular level. Results obtained from measurements in an equimolar mixture of zingerone and curcumin indicate synergism (p < 0.05) between the two compounds. This combination is especially successful due to the fast and efficient neutralization of added pro-oxidant TBH. The commercial availability of turmeric and ginger and their frequent combined use in diet suggest ideas for further broader utilization of the beneficial synergistic effect of their phenolic components.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls",
volume = "28",
number = "6",
doi = "10.3390/molecules28062646"
}

Kostić, K., Brborić, J., Delogu, G., Simić, M., Samardžić, S., Maksimović, Z., Dettori, M. A., Fabbri, D., Kotur-Stevuljević, J.,& Saso, L.. (2023). Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls. in Molecules
MDPI., 28(6).
https://doi.org/10.3390/molecules28062646

Kostić K, Brborić J, Delogu G, Simić M, Samardžić S, Maksimović Z, Dettori MA, Fabbri D, Kotur-Stevuljević J, Saso L. Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls. in Molecules. 2023;28(6).
doi:10.3390/molecules28062646 .

Kostić, Kristina, Brborić, Jasmina, Delogu, Giovanna, Simić, Milena, Samardžić, Stevan, Maksimović, Zoran, Dettori, Maria Antonietta, Fabbri, Davide, Kotur-Stevuljević, Jelena, Saso, Luciano, "Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls" in Molecules, 28, no. 6 (2023),
https://doi.org/10.3390/molecules28062646 . .

TY  - CONF
AU  - Leković, Aleksandra
AU  - Samardžić, Stevan
AU  - Radović-Selgrad, Jelena
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4584
AB  - Sorbus torminalis (L.) Crantz (Rosaceae), also known as wild service tree, is a
deciduous tree widespread in central and southern Europe, north-western Africa, western
Asia, the Balkan Peninsula and the Caucasus (1, 2). Species of the genus Sorbus represent a
rich source of polyphenols that are responsible for their numerous proven effects, including
anti-diarrhoeal, anti-inflammatory, antidiabetic, antioxidant and anti-atherogenic effect.
Accordingly, the bark of S. torminalis could have interesting composition and effects,
however, data about this herbal drug is insufficient (1). Bark dry methanol extract (DER
9.74:1), obtained after successive extraction with cyclohexane and dichloromethane, was
analyzed for antioxidant potential using DPPH and FRAP assay. Contents of total
polyphenols, flavonoids and tannins were determined using spectrophotometric methods
based on reaction with Folin-Ciocalteu reagent (FC), reaction with aluminum-chloride and
adsorption by hide powder in combination with reaction with FC, respectively. LC-DAD-ESI-
MS analysis was used for identification of main extract constituents. In DPPH test IC50 value
8.66±0.07 μg/mL was shown, while result of FRAP test was 3.01±0.11 μmol Fe 2+ /mg of dry
extract. Contents of total polyphenols, flavonoids and tannins were 0.39±0.06 mg GA/mg of
dry extract, 0.035±0.019% and 1.3±0.08%, respectively. By LC-DAD-ESI-MS analysis 25
compounds were detected among which procyanidins (dimers, trimers and tetramers) are
dominant. The results of this study showed that dry methanol extract of S. torminalis bark is
rich in procyanidins which potentially contribute to its antioxidant activity. Additional
significant effects of this herbal drug and its isolated compounds should be investigated in
future studies.
AB  - Sorbus torminalis (L.) Crantz (Rosaceae) je listopadno drvo, u narodu poznato pod
nazivom brekinja. Rasprostranjeno je širom centralne i južne Evrope, severozapadne Afrike,
zapadne Azije, Balkanskog poluostrva i Kavkaza (1, 2). Vrste roda Sorbus predstavljaju bogat
izvor polifenola koji su odgovorni za njihove brojne dokazane efekte, uključuju antidijaroični,
antiinflamatorni, antidijabetski, antioksidantni i antiaterogeni efekat. U skladu sa tim, kora S.
torminalis mogla bi imati interesantan sastav i efekte, međutim, o ovoj biljnoj drogi nema
dovoljno podataka (1). Antioksidantna aktivnost suvog metanolnog ekstrakta kore stabla
(DER 9,74:1), dobijenog nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
analizirana je DPPH i FRAP testom. Sadržaji ukupnih polifenola, flavonoida i tanina određeni
su spektrofotometrijskim metodama koje se zasnivaju na reakciji sa Folin‐Ciocalteu
reagensom (FC), reakciji sa aluminijum-hloridom i na adsorpciji na kožni prah u kombinaciji
sa reakcijom sa FC reagensom, redom. LC‐DAD‐ESI‐MS analiza korišćena je za identifikaciju
glavnih sastojaka ekstrakta. U DPPH testu dobijena je IC50 vrednost 8,66±0,07 μg/mL, a
rezultat FRAP testa bio je 3,01±0,11 μmol Fe 2+ /mg suvog ekstrakta. Sadžaj ukupnih
polifenola bio je 0,39±0,06 mg GA/mg suvog ekstrakta, flavonoida 0,035±0,019%, a tanina
1,3±0,08%. LC‐DAD‐ESI‐MS analizom detektovano je 25 jedinjenja od kojih su dominantni
procijanidini (dimeri, trimeri i tetrameri). Rezultati ovog istraživanja pokazali su da je suvi
metanolni ekstrakt kore stabla S. torminalis bogat procijanidinima koji potencijalno
doprinose njegovoj antioksidantnoj aktivnosti. Dodatne značajne efekte ove biljne droge i
njenih izolovanih jedinjenja trebalo bi ispitati u budućim istraživanjima.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract
T1  - Hemijski sastav i antioksidantna aktivnost metanolnog ekstrakta kore stabla Sorbus torminalis
VL  - 72
IS  - 4 suplement
SP  - S467
EP  - S468
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4584
ER  - 

@conference{
author = "Leković, Aleksandra and Samardžić, Stevan and Radović-Selgrad, Jelena and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Sorbus torminalis (L.) Crantz (Rosaceae), also known as wild service tree, is a
deciduous tree widespread in central and southern Europe, north-western Africa, western
Asia, the Balkan Peninsula and the Caucasus (1, 2). Species of the genus Sorbus represent a
rich source of polyphenols that are responsible for their numerous proven effects, including
anti-diarrhoeal, anti-inflammatory, antidiabetic, antioxidant and anti-atherogenic effect.
Accordingly, the bark of S. torminalis could have interesting composition and effects,
however, data about this herbal drug is insufficient (1). Bark dry methanol extract (DER
9.74:1), obtained after successive extraction with cyclohexane and dichloromethane, was
analyzed for antioxidant potential using DPPH and FRAP assay. Contents of total
polyphenols, flavonoids and tannins were determined using spectrophotometric methods
based on reaction with Folin-Ciocalteu reagent (FC), reaction with aluminum-chloride and
adsorption by hide powder in combination with reaction with FC, respectively. LC-DAD-ESI-
MS analysis was used for identification of main extract constituents. In DPPH test IC50 value
8.66±0.07 μg/mL was shown, while result of FRAP test was 3.01±0.11 μmol Fe 2+ /mg of dry
extract. Contents of total polyphenols, flavonoids and tannins were 0.39±0.06 mg GA/mg of
dry extract, 0.035±0.019% and 1.3±0.08%, respectively. By LC-DAD-ESI-MS analysis 25
compounds were detected among which procyanidins (dimers, trimers and tetramers) are
dominant. The results of this study showed that dry methanol extract of S. torminalis bark is
rich in procyanidins which potentially contribute to its antioxidant activity. Additional
significant effects of this herbal drug and its isolated compounds should be investigated in
future studies., Sorbus torminalis (L.) Crantz (Rosaceae) je listopadno drvo, u narodu poznato pod
nazivom brekinja. Rasprostranjeno je širom centralne i južne Evrope, severozapadne Afrike,
zapadne Azije, Balkanskog poluostrva i Kavkaza (1, 2). Vrste roda Sorbus predstavljaju bogat
izvor polifenola koji su odgovorni za njihove brojne dokazane efekte, uključuju antidijaroični,
antiinflamatorni, antidijabetski, antioksidantni i antiaterogeni efekat. U skladu sa tim, kora S.
torminalis mogla bi imati interesantan sastav i efekte, međutim, o ovoj biljnoj drogi nema
dovoljno podataka (1). Antioksidantna aktivnost suvog metanolnog ekstrakta kore stabla
(DER 9,74:1), dobijenog nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
analizirana je DPPH i FRAP testom. Sadržaji ukupnih polifenola, flavonoida i tanina određeni
su spektrofotometrijskim metodama koje se zasnivaju na reakciji sa Folin‐Ciocalteu
reagensom (FC), reakciji sa aluminijum-hloridom i na adsorpciji na kožni prah u kombinaciji
sa reakcijom sa FC reagensom, redom. LC‐DAD‐ESI‐MS analiza korišćena je za identifikaciju
glavnih sastojaka ekstrakta. U DPPH testu dobijena je IC50 vrednost 8,66±0,07 μg/mL, a
rezultat FRAP testa bio je 3,01±0,11 μmol Fe 2+ /mg suvog ekstrakta. Sadžaj ukupnih
polifenola bio je 0,39±0,06 mg GA/mg suvog ekstrakta, flavonoida 0,035±0,019%, a tanina
1,3±0,08%. LC‐DAD‐ESI‐MS analizom detektovano je 25 jedinjenja od kojih su dominantni
procijanidini (dimeri, trimeri i tetrameri). Rezultati ovog istraživanja pokazali su da je suvi
metanolni ekstrakt kore stabla S. torminalis bogat procijanidinima koji potencijalno
doprinose njegovoj antioksidantnoj aktivnosti. Dodatne značajne efekte ove biljne droge i
njenih izolovanih jedinjenja trebalo bi ispitati u budućim istraživanjima.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract, Hemijski sastav i antioksidantna aktivnost metanolnog ekstrakta kore stabla Sorbus torminalis",
volume = "72",
number = "4 suplement",
pages = "S467-S468",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4584"
}

Leković, A., Samardžić, S., Radović-Selgrad, J.,& Kundaković-Vasović, T.. (2022). Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S467-S468.
https://hdl.handle.net/21.15107/rcub_farfar_4584

Leković A, Samardžić S, Radović-Selgrad J, Kundaković-Vasović T. Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract. in Arhiv za farmaciju. 2022;72(4 suplement):S467-S468.
https://hdl.handle.net/21.15107/rcub_farfar_4584 .

Leković, Aleksandra, Samardžić, Stevan, Radović-Selgrad, Jelena, Kundaković-Vasović, Tatjana, "Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S467-S468,
https://hdl.handle.net/21.15107/rcub_farfar_4584 .

TY  - CONF
AU  - Božić, Dragana
AU  - Ilić, Tijana
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4616
AB  - In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - In vitro assessment of the prebiotic potential of selected plant extracts
SP  - 54
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4616
ER  - 

@conference{
author = "Božić, Dragana and Ilić, Tijana and Marčetić, Mirjana and Samardžić, Stevan and Zdunić, Gordana and Vidović, Bojana",
year = "2022",
abstract = "In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "In vitro assessment of the prebiotic potential of selected plant extracts",
pages = "54-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4616"
}

Božić, D., Ilić, T., Marčetić, M., Samardžić, S., Zdunić, G.,& Vidović, B.. (2022). In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616

Božić D, Ilić T, Marčetić M, Samardžić S, Zdunić G, Vidović B. In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

Božić, Dragana, Ilić, Tijana, Marčetić, Mirjana, Samardžić, Stevan, Zdunić, Gordana, Vidović, Bojana, "In vitro assessment of the prebiotic potential of selected plant extracts" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):54-54,
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

TY  - CONF
AU  - Ilić, Tijana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Božić, Dragana
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4617
AB  - Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4617
ER  - 

@conference{
author = "Ilić, Tijana and Samardžić, Stevan and Zdunić, Gordana and Božić, Dragana and Marčetić, Mirjana and Vidović, Bojana",
year = "2022",
abstract = "Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4617"
}

Ilić, T., Samardžić, S., Zdunić, G., Božić, D., Marčetić, M.,& Vidović, B.. (2022). Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617

Ilić T, Samardžić S, Zdunić G, Božić D, Marčetić M, Vidović B. Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

Ilić, Tijana, Samardžić, Stevan, Zdunić, Gordana, Božić, Dragana, Marčetić, Mirjana, Vidović, Bojana, "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):44-44,
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4610
AB  - The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae
SP  - 167
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4610
ER  - 

@conference{
author = "Marčetić, Mirjana and Vidović, Bojana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana",
year = "2022",
abstract = "The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4610"
}

Marčetić, M., Vidović, B., Samardžić, S., Ilić, T.,& Božić, D.. (2022). Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610

Marčetić M, Vidović B, Samardžić S, Ilić T, Božić D. Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

Marčetić, Mirjana, Vidović, Bojana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):167-167,
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4311
AB  - Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.
PB  - MDPI
T2  - Foods
T1  - Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits
VL  - 11
IS  - 20
DO  - 10.3390/foods11203289
ER  - 

@article{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2022",
abstract = "Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.",
publisher = "MDPI",
journal = "Foods",
title = "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits",
volume = "11",
number = "20",
doi = "10.3390/foods11203289"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2022). Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods
MDPI., 11(20).
https://doi.org/10.3390/foods11203289

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods. 2022;11(20).
doi:10.3390/foods11203289 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits" in Foods, 11, no. 20 (2022),
https://doi.org/10.3390/foods11203289 . .

TY  - JOUR
AU  - Romanić, Ranko
AU  - Lužaić, Tanja
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5252
AB  - Usled stalnog porasta broja stanovnika raste i potreba za hranom u svetu, što dovodi do povećavanja obradivih površine pod žitaricama i uljaricama. Raste i količina žetvenih ostataka koji se najčešće spaljuju. Spaljivanje žetvenih ostataka predstavlja veliki ekološki rizik, sa jedne strane, jer je čest uzročnik požara, dok sa druge strane predstavlja vrednu biomasu koja ostaje neiskorišćena. U poslednjih nekoliko godina je primećen trend spaljivanja ostataka na polju što dovodi do zagađenja vazduha i predstavlja opasnost po zdravlje stanovništva. Žetveni ostaci sadrže različite komponente koje bi mogle naći svoju primenu u prehramebnoj i farmaceutskoj industriji. Analizom sastava lipidnog ekstrakta žetvenih ostataka utvrđeno je prisustvo biološki vrednih komponenata koje dalje mogu naći svoju primenu u proizvodnji mesnih prerađevina sa poboljšanom oksidativnom stabilnošću, boljom održivošću, poboljšanim sastavom masnih kiselina, kao i novim formulama prirodne kozmetike.
T2  - Uljarstvo
T1  - Sastav lipidnog ekstrakta žetvenih ostataka pšenice, kukuruza i suncokreta
T1  - Composition of lipid extract of wheat, corn and sunflower harvest residues
VL  - 53
IS  - 1
SP  - 67
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5252
ER  - 

@article{
author = "Romanić, Ranko and Lužaić, Tanja and Samardžić, Stevan and Maksimović, Zoran",
year = "2022",
abstract = "Usled stalnog porasta broja stanovnika raste i potreba za hranom u svetu, što dovodi do povećavanja obradivih površine pod žitaricama i uljaricama. Raste i količina žetvenih ostataka koji se najčešće spaljuju. Spaljivanje žetvenih ostataka predstavlja veliki ekološki rizik, sa jedne strane, jer je čest uzročnik požara, dok sa druge strane predstavlja vrednu biomasu koja ostaje neiskorišćena. U poslednjih nekoliko godina je primećen trend spaljivanja ostataka na polju što dovodi do zagađenja vazduha i predstavlja opasnost po zdravlje stanovništva. Žetveni ostaci sadrže različite komponente koje bi mogle naći svoju primenu u prehramebnoj i farmaceutskoj industriji. Analizom sastava lipidnog ekstrakta žetvenih ostataka utvrđeno je prisustvo biološki vrednih komponenata koje dalje mogu naći svoju primenu u proizvodnji mesnih prerađevina sa poboljšanom oksidativnom stabilnošću, boljom održivošću, poboljšanim sastavom masnih kiselina, kao i novim formulama prirodne kozmetike.",
journal = "Uljarstvo",
title = "Sastav lipidnog ekstrakta žetvenih ostataka pšenice, kukuruza i suncokreta, Composition of lipid extract of wheat, corn and sunflower harvest residues",
volume = "53",
number = "1",
pages = "67-74",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5252"
}

Romanić, R., Lužaić, T., Samardžić, S.,& Maksimović, Z.. (2022). Sastav lipidnog ekstrakta žetvenih ostataka pšenice, kukuruza i suncokreta. in Uljarstvo, 53(1), 67-74.
https://hdl.handle.net/21.15107/rcub_farfar_5252

Romanić R, Lužaić T, Samardžić S, Maksimović Z. Sastav lipidnog ekstrakta žetvenih ostataka pšenice, kukuruza i suncokreta. in Uljarstvo. 2022;53(1):67-74.
https://hdl.handle.net/21.15107/rcub_farfar_5252 .

Romanić, Ranko, Lužaić, Tanja, Samardžić, Stevan, Maksimović, Zoran, "Sastav lipidnog ekstrakta žetvenih ostataka pšenice, kukuruza i suncokreta" in Uljarstvo, 53, no. 1 (2022):67-74,
https://hdl.handle.net/21.15107/rcub_farfar_5252 .

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kotur-Stevuljević, Jelena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4618
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4618
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kotur-Stevuljević, Jelena and Samardžić, Stevan and Maksimović, Zoran",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4618"
}

Spremo-Potparević, B., Živković, L., Topalović, D., Kotur-Stevuljević, J., Samardžić, S.,& Maksimović, Z.. (2022). Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618

Spremo-Potparević B, Živković L, Topalović D, Kotur-Stevuljević J, Samardžić S, Maksimović Z. Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618 .

Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kotur-Stevuljević, Jelena, Samardžić, Stevan, Maksimović, Zoran, "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):90-90,
https://hdl.handle.net/21.15107/rcub_farfar_4618 .

TY  - CONF
AU  - Suručić, Relja
AU  - Samardžić, Stevan
AU  - Radulović, Dragana
AU  - Lakić, Željko
AU  - Travar, Nikola
AU  - Antić, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4986
AB  - Geranium robertianum L. (Geraniaceae) has been traditionally used to treat a
range of ailments, including high blood pressure Recent in vitro studies have
shown that some traditional usages, such as antimicrobials, were scientifically
confirmed. Although the phytoconstituents of this plant species have been
extensively studied, some plant parts used in traditional medicine, such as
flowers, have not yet been chemically characterized. Given that the inhibition
of the angiotensin converting enzyme (ACE) is one of the most significant
mechanisms for decreasing blood pressure, we undertook this investigation to
find scientific support for G. robertianum anti-hypertensive traditional use.
Acetone was used to extract plant material since prior research revealed that it
had the highest total phenol and total flavonoid levels, which are considered to
be the primary sources of bioactive chemicals. The chemical composition of
G. robertianum collected in R. Srpska was determined using the LC MS
method, and the ACE inhibitory activity of acetone extract was measured
through the enzymatically cleaved 3-hydroxybutyric acid from 3-
hydryoxybutyryl-gly-gly-gly. Geraniin, a hydrolysable tannin with a Mr of
952.6, was the most abundant single chemical found in the extract. Derivatives
of galic and ellagic acids, as well as flavonoids like kaempferol and quercetine,
a were also present in significant amounts. G. robertianum acetonic floral
extract has been shown to be an effective natural ACE inhibitor with an IC50
of 22.14 μg/mL. To the best of our knowledge, this is the first report on the
chemical composition of G. robertianum floral acetonic extract and its ACE
inhibitory activity.
PB  - Faculty of Agriculture, University of Banja Luka
C3  - XI International Symposium of Agricultural Sciences "AgroReS 2022" - Book of Abstracts
T1  - Chemical characterization and ACE-inhibitory activity of acetone extract of Geranium robertianum L. flowers
SP  - 153
EP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4986
ER  - 

@conference{
author = "Suručić, Relja and Samardžić, Stevan and Radulović, Dragana and Lakić, Željko and Travar, Nikola and Antić, Marina",
year = "2022",
abstract = "Geranium robertianum L. (Geraniaceae) has been traditionally used to treat a
range of ailments, including high blood pressure Recent in vitro studies have
shown that some traditional usages, such as antimicrobials, were scientifically
confirmed. Although the phytoconstituents of this plant species have been
extensively studied, some plant parts used in traditional medicine, such as
flowers, have not yet been chemically characterized. Given that the inhibition
of the angiotensin converting enzyme (ACE) is one of the most significant
mechanisms for decreasing blood pressure, we undertook this investigation to
find scientific support for G. robertianum anti-hypertensive traditional use.
Acetone was used to extract plant material since prior research revealed that it
had the highest total phenol and total flavonoid levels, which are considered to
be the primary sources of bioactive chemicals. The chemical composition of
G. robertianum collected in R. Srpska was determined using the LC MS
method, and the ACE inhibitory activity of acetone extract was measured
through the enzymatically cleaved 3-hydroxybutyric acid from 3-
hydryoxybutyryl-gly-gly-gly. Geraniin, a hydrolysable tannin with a Mr of
952.6, was the most abundant single chemical found in the extract. Derivatives
of galic and ellagic acids, as well as flavonoids like kaempferol and quercetine,
a were also present in significant amounts. G. robertianum acetonic floral
extract has been shown to be an effective natural ACE inhibitor with an IC50
of 22.14 μg/mL. To the best of our knowledge, this is the first report on the
chemical composition of G. robertianum floral acetonic extract and its ACE
inhibitory activity.",
publisher = "Faculty of Agriculture, University of Banja Luka",
journal = "XI International Symposium of Agricultural Sciences "AgroReS 2022" - Book of Abstracts",
title = "Chemical characterization and ACE-inhibitory activity of acetone extract of Geranium robertianum L. flowers",
pages = "153-154",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4986"
}

Suručić, R., Samardžić, S., Radulović, D., Lakić, Ž., Travar, N.,& Antić, M.. (2022). Chemical characterization and ACE-inhibitory activity of acetone extract of Geranium robertianum L. flowers. in XI International Symposium of Agricultural Sciences "AgroReS 2022" - Book of Abstracts
Faculty of Agriculture, University of Banja Luka., 153-154.
https://hdl.handle.net/21.15107/rcub_farfar_4986

Suručić R, Samardžić S, Radulović D, Lakić Ž, Travar N, Antić M. Chemical characterization and ACE-inhibitory activity of acetone extract of Geranium robertianum L. flowers. in XI International Symposium of Agricultural Sciences "AgroReS 2022" - Book of Abstracts. 2022;:153-154.
https://hdl.handle.net/21.15107/rcub_farfar_4986 .

Suručić, Relja, Samardžić, Stevan, Radulović, Dragana, Lakić, Željko, Travar, Nikola, Antić, Marina, "Chemical characterization and ACE-inhibitory activity of acetone extract of Geranium robertianum L. flowers" in XI International Symposium of Agricultural Sciences "AgroReS 2022" - Book of Abstracts (2022):153-154,
https://hdl.handle.net/21.15107/rcub_farfar_4986 .

TY  - CONF
AU  - Jakovljević, Dunja
AU  - Samardžić, Stevan
AU  - Majkić, Tatjana
AU  - Beara, Ivana
AU  - Maksimović, Zoran
AU  - Ćebović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4586
AB  - Curly dock (Rumex crispus L.) is a perennial herbaceous plant that is traditionally used
in the treatment of gastrointestinal ailments, infections and skin diseases. The aim of this
study was to examine the chemical composition, ex vivo anti-inflammatory effect and in vivo
antiproliferative activity of water extract of curly dock fruit. The polyphenolic profile was
analyzed by LC-DAD-ESI-MS technique. The effect on the production of arachidonic acid
metabolites was examined in human platelets. Antitumor activity of orally administered
extract (concentration: 5% w/v; doses: 1, 2 and 5 mL/kg of body weight/day; treatment
length: 7 days) to Ehrlich ascites tumor and hepatocellular carcinoma was assessed in an
animal experiment. Ascites volume, viable cell percentage and antioxidant enzyme levels
were determined. The presence of phenolic acids (gallic and protocatechuic acids),
proanthocyanidins (monomers, dimers and trimers), flavonoid glycosides and
anthraquinone emodin was revealed in the water extract of R. crispus fruit. The content of
the quantitatively dominant compound miquelianin was 2.4%. The investigated preparation
concentration-dependently inhibited the synthesis of 12(S)-hydroxy-(5Z,8E,10E)-
heptadecatrienoic acid (IC50 = 4.96 mg/mL) and 12(S)-hydroxy-(5Z,8Z,10E,14Z)-
eicosatetraenoic acid (IC 50 = 2.06 mg/mL). The antitumor effect in animals was dose-
dependent and manifested as suppression of tumor growth and induction of cell death of
malignantly transformed cells. Values of biochemical parameters suggested that oxidative
stress was induced in cancer cells, and it can be assumed that this mechanism was at least
partly responsible for the activity. Water extract of R. crispus fruit has significant
pharmacological potential that justifies further research.
AB  - Štavelj (Rumex crispus L.) je višegodišnja zeljasta biljka koja se tradicionalno koristi u
tretmanu gastrointestinalnih tegoba, infekcija i kožnih oboljenja. Cilj ove studije je bio da se
ispita hemijski sastav, ex vivo antiinflamatorni efekat i in vivo antiproliferativna aktivnost
vodenog ekstrakta ploda štavelja. Polifenolni profil je analiziran LC‐DAD‐ESI‐MS tehnikom.
Uticaj na produkciju metabolita arahidonske kiseline je ispitan u humanim trombocitima.
Antitumorska aktivnost oralno primenjenog ekstrakta (koncentracija: 5% m/V; doze: 1, 2 i 5
mL/kg telesne težine/dan; dužina tretmana: 7 dana) prema Ehrlich-ovom ascitnom tumoru i
hepatocelularnom karcinomu procenjena je u eksperimentu na životinjama. Određeni su
zapremina ascitesa, procenat vijabilnih ćelija i nivoi antioksidantnih enzima. U vodenom
ekstraktu ploda R. crispus je utvrđeno prisustvo fenolnih kiselina (galna i protokatehinska
kiselina), proantocijanidina (monomeri, dimeri i trimeri), flavonoidnih heterozida i
antrahinona emodina. Sadržaj kvantitativno dominantnog jedinjenja mikvelijanina je iznosio
2,4%. Ispitivani preparat je koncentraciono-zavisno inhibirao sintezu 12(S)-hidroksi-
(5Z,8E,10E)-heptadekatrienske kiseline (IC 50 = 4,96 mg/mL) i 12(S)-hidroksi-
(5Z,8Z,10E,14Z)-eikozatetraenske kiseline (IC50 = 2,06 mg/mL). Antitumorski efekat u
životinja je bio dozno-zavisan i ispoljio se kao suprimiranje rasta tumora i indukcija ćelijske
smrti maligno transformisanih ćelija. Vrednosti biohemijskih parametara su sugerisale da je
u ćelijama karcinoma došlo do indukovanja oksidativnog stresa, te se može pretpostaviti da
je ovaj mehanizam bio barem delom zaslužan za aktivnost. Vodeni ekstrakt ploda R. crispus
poseduje značajan farmakološki potencijal koji opravdava dalje istraživanje.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae)
T1  - Hemijski sastav, antiinflamatorni efekat i antitumorska aktivnost ploda štavelja (Rumex crispus L., Polygonaceae)
VL  - 72
IS  - 4 suplement
SP  - S479
EP  - S480
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4586
ER  - 

@conference{
author = "Jakovljević, Dunja and Samardžić, Stevan and Majkić, Tatjana and Beara, Ivana and Maksimović, Zoran and Ćebović, Tatjana",
year = "2022",
abstract = "Curly dock (Rumex crispus L.) is a perennial herbaceous plant that is traditionally used
in the treatment of gastrointestinal ailments, infections and skin diseases. The aim of this
study was to examine the chemical composition, ex vivo anti-inflammatory effect and in vivo
antiproliferative activity of water extract of curly dock fruit. The polyphenolic profile was
analyzed by LC-DAD-ESI-MS technique. The effect on the production of arachidonic acid
metabolites was examined in human platelets. Antitumor activity of orally administered
extract (concentration: 5% w/v; doses: 1, 2 and 5 mL/kg of body weight/day; treatment
length: 7 days) to Ehrlich ascites tumor and hepatocellular carcinoma was assessed in an
animal experiment. Ascites volume, viable cell percentage and antioxidant enzyme levels
were determined. The presence of phenolic acids (gallic and protocatechuic acids),
proanthocyanidins (monomers, dimers and trimers), flavonoid glycosides and
anthraquinone emodin was revealed in the water extract of R. crispus fruit. The content of
the quantitatively dominant compound miquelianin was 2.4%. The investigated preparation
concentration-dependently inhibited the synthesis of 12(S)-hydroxy-(5Z,8E,10E)-
heptadecatrienoic acid (IC50 = 4.96 mg/mL) and 12(S)-hydroxy-(5Z,8Z,10E,14Z)-
eicosatetraenoic acid (IC 50 = 2.06 mg/mL). The antitumor effect in animals was dose-
dependent and manifested as suppression of tumor growth and induction of cell death of
malignantly transformed cells. Values of biochemical parameters suggested that oxidative
stress was induced in cancer cells, and it can be assumed that this mechanism was at least
partly responsible for the activity. Water extract of R. crispus fruit has significant
pharmacological potential that justifies further research., Štavelj (Rumex crispus L.) je višegodišnja zeljasta biljka koja se tradicionalno koristi u
tretmanu gastrointestinalnih tegoba, infekcija i kožnih oboljenja. Cilj ove studije je bio da se
ispita hemijski sastav, ex vivo antiinflamatorni efekat i in vivo antiproliferativna aktivnost
vodenog ekstrakta ploda štavelja. Polifenolni profil je analiziran LC‐DAD‐ESI‐MS tehnikom.
Uticaj na produkciju metabolita arahidonske kiseline je ispitan u humanim trombocitima.
Antitumorska aktivnost oralno primenjenog ekstrakta (koncentracija: 5% m/V; doze: 1, 2 i 5
mL/kg telesne težine/dan; dužina tretmana: 7 dana) prema Ehrlich-ovom ascitnom tumoru i
hepatocelularnom karcinomu procenjena je u eksperimentu na životinjama. Određeni su
zapremina ascitesa, procenat vijabilnih ćelija i nivoi antioksidantnih enzima. U vodenom
ekstraktu ploda R. crispus je utvrđeno prisustvo fenolnih kiselina (galna i protokatehinska
kiselina), proantocijanidina (monomeri, dimeri i trimeri), flavonoidnih heterozida i
antrahinona emodina. Sadržaj kvantitativno dominantnog jedinjenja mikvelijanina je iznosio
2,4%. Ispitivani preparat je koncentraciono-zavisno inhibirao sintezu 12(S)-hidroksi-
(5Z,8E,10E)-heptadekatrienske kiseline (IC 50 = 4,96 mg/mL) i 12(S)-hidroksi-
(5Z,8Z,10E,14Z)-eikozatetraenske kiseline (IC50 = 2,06 mg/mL). Antitumorski efekat u
životinja je bio dozno-zavisan i ispoljio se kao suprimiranje rasta tumora i indukcija ćelijske
smrti maligno transformisanih ćelija. Vrednosti biohemijskih parametara su sugerisale da je
u ćelijama karcinoma došlo do indukovanja oksidativnog stresa, te se može pretpostaviti da
je ovaj mehanizam bio barem delom zaslužan za aktivnost. Vodeni ekstrakt ploda R. crispus
poseduje značajan farmakološki potencijal koji opravdava dalje istraživanje.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae), Hemijski sastav, antiinflamatorni efekat i antitumorska aktivnost ploda štavelja (Rumex crispus L., Polygonaceae)",
volume = "72",
number = "4 suplement",
pages = "S479-S480",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4586"
}

Jakovljević, D., Samardžić, S., Majkić, T., Beara, I., Maksimović, Z.,& Ćebović, T.. (2022). Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S479-S480.
https://hdl.handle.net/21.15107/rcub_farfar_4586

Jakovljević D, Samardžić S, Majkić T, Beara I, Maksimović Z, Ćebović T. Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae). in Arhiv za farmaciju. 2022;72(4 suplement):S479-S480.
https://hdl.handle.net/21.15107/rcub_farfar_4586 .

Jakovljević, Dunja, Samardžić, Stevan, Majkić, Tatjana, Beara, Ivana, Maksimović, Zoran, Ćebović, Tatjana, "Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae)" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S479-S480,
https://hdl.handle.net/21.15107/rcub_farfar_4586 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5097
AB  - The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.
PB  - University of Belgrade
C3  - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
T1  - Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5097
ER  - 

@conference{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2021",
abstract = "The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.",
publisher = "University of Belgrade",
journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts",
title = "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5097"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2021). Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
University of Belgrade., 85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):85-85,
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

TY  - JOUR
AU  - Mićović, Tijana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Jakovljević, Vladimir
AU  - Matić, Sanja
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Stešević, Danijela
AU  - Samardžić, Stevan
AU  - Stojanović, Danilo
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3817
AB  - Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.
PB  - MDPI AG
T2  - Plants
T1  - Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)
VL  - 10
IS  - 4
DO  - 10.3390/plants10040711
ER  - 

@article{
author = "Mićović, Tijana and Topalović, Dijana and Živković, Lada and Spremo-Potparević, Biljana and Jakovljević, Vladimir and Matić, Sanja and Popović, Suzana and Baskić, Dejan and Stešević, Danijela and Samardžić, Stevan and Stojanović, Danilo and Maksimović, Zoran",
year = "2021",
abstract = "Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)",
volume = "10",
number = "4",
doi = "10.3390/plants10040711"
}

Mićović, T., Topalović, D., Živković, L., Spremo-Potparević, B., Jakovljević, V., Matić, S., Popović, S., Baskić, D., Stešević, D., Samardžić, S., Stojanović, D.,& Maksimović, Z.. (2021). Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants
MDPI AG., 10(4).
https://doi.org/10.3390/plants10040711

Mićović T, Topalović D, Živković L, Spremo-Potparević B, Jakovljević V, Matić S, Popović S, Baskić D, Stešević D, Samardžić S, Stojanović D, Maksimović Z. Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants. 2021;10(4).
doi:10.3390/plants10040711 .

Mićović, Tijana, Topalović, Dijana, Živković, Lada, Spremo-Potparević, Biljana, Jakovljević, Vladimir, Matić, Sanja, Popović, Suzana, Baskić, Dejan, Stešević, Danijela, Samardžić, Stevan, Stojanović, Danilo, Maksimović, Zoran, "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)" in Plants, 10, no. 4 (2021),
https://doi.org/10.3390/plants10040711 . .

TY  - CONF
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
AU  - Đorđić, Marija
AU  - Stanojković, Tatjana
AU  - Petković, Miloš
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5104
AB  - INTRODUCTION:
False indigo-bush (Amorpha fruticosa L., Fabaceae) is an invasive shrub native to central and eastern North America. In Serbia,
it can be found along roads, railways, and riverbeds, where it spreads easily. Previous studies have shown that A. fruticosa
contains several classes of secondary metabolites (e.g. rotenoids, isoflavonoids, prenylflavanones, chalcones, and stilbe-
noids) and exhibits various biological activities (e.g. antioxidant, anti-inflammatory, antidiabetic, insecticidal).
OBJECTIVES:
This study aimed to examine the in vitro cytotoxic activity of prenylated phenolic constituents of false indigo-bush.
METHOD / DESIGN:
An aliquot (0.82 g) of ethanol-soluble part of n-hexane fruit extract (1:10, m/V) was loaded onto silica gel column (3 × 43 cm)
and eluted with mixtures of n-hexane and ethyl acetate (100:0 _ 0:100, V/V). 96 Fractions were collected, analyzed by silica
gel TLC and combined based on chemical composition similarity. Compound 1 (6.9 mg) precipitated as the white powder
during solvent removal from the united fractions 59–69. The pooled fractions 89–92 (80.6 mg) and 95–96 (59.6 mg) gave
compounds 2 (29.8 mg, Rf = 0.5) and 3 (5.8 mg, Rf = 0.53), respectively, after separation by preparative silica gel TLC in a
solvent system consisting of n-hexane, ethyl acetate and formic acid (80:20:1, V/V/V). The structure determination of the iso-
lated prenylated phenols were based on their UV, MS, and 1
H and 13
C NMR spectra. MTT assay was employed to assess their
cytotoxicity against human tumor cell lines (HeLa, HT-29
, HCT-116
, LS174) derived from the cervix adenocarcinoma and colorec-
tal carcinomas, as well as human fetal lung fibroblasts (MRC-5). cis-Diamminedichloroplatinum (CDDP) was used as a control
substance. The activity is expressed as the IC 50 value, i.e. the concentration of the tested substance that led to 50% inhibition
of survival, compared to the vehicle-treated control group. The statistical analysis was performed using the Student’s t-test.
RESULTS:
Based on a comparison of recorded spectral data and literature data, compound 1 was identified as 5,7-dihydroxy-8-gera-
nylflavanone, compound 2 as 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl, and compound 3 as 2-carboxy-3-hydroxy-4-pre-
nyl-5-metoxybibenzyl (amorfrutin A). All tested constituents showed cytotoxicity with the IC50 values in the range 10.55–
166.11 μg/mL. The non-selectivity of compounds 2 and 3 could be observed. On the other hand, compound 1 was selective
and exhibited pronounced activity against the HeLa cell line (IC 50 = 10.55 μg/mL). HT-29 (IC 50 = 122.28 μg/mL) and HCT-116
(IC 50 = 147.09 μg/mL) cancer cell lines, as well as normal cell line MRC-5 (IC50 = 166.11 μg/mL), were less susceptible to the action of compound 1, whereas the survival of LS174 cell line was not affected at all by compound 1 in the tested concen-
tration range. The effectiveness of the control substance CDDP in reducing the survival of the HeLa cancer cell line was the
highest (IC50 = 5.20 μg/mL). It should be emphasized that CDDP, among tested substances, was also the most toxic against
the MRC-5 cell line (IC 50 = 8.28 μg/mL). IC 50 values ranges of compounds 2 and 3 were 31.97–56.29 μg/mL and 35.67–53.21
μg/mL, respectively.
CONCLUSIONS:
5,7-Dihydroxy-8-geranylflavanone, the constituent of false indigo-bush fruit, exhibited strong and selective activity against
the human cervix adenocarcinoma cell line in the MTT assay, therefore, its cytotoxic potential can be considered significant.
Further studies are needed to fully assess the demonstrated effect.
PB  - University Prince of Songkla, Thailand
PB  - University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
T1  - Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)
SP  - 126
EP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5104
ER  - 

@conference{
author = "Samardžić, Stevan and Maksimović, Zoran and Đorđić, Marija and Stanojković, Tatjana and Petković, Miloš",
year = "2021",
abstract = "INTRODUCTION:
False indigo-bush (Amorpha fruticosa L., Fabaceae) is an invasive shrub native to central and eastern North America. In Serbia,
it can be found along roads, railways, and riverbeds, where it spreads easily. Previous studies have shown that A. fruticosa
contains several classes of secondary metabolites (e.g. rotenoids, isoflavonoids, prenylflavanones, chalcones, and stilbe-
noids) and exhibits various biological activities (e.g. antioxidant, anti-inflammatory, antidiabetic, insecticidal).
OBJECTIVES:
This study aimed to examine the in vitro cytotoxic activity of prenylated phenolic constituents of false indigo-bush.
METHOD / DESIGN:
An aliquot (0.82 g) of ethanol-soluble part of n-hexane fruit extract (1:10, m/V) was loaded onto silica gel column (3 × 43 cm)
and eluted with mixtures of n-hexane and ethyl acetate (100:0 _ 0:100, V/V). 96 Fractions were collected, analyzed by silica
gel TLC and combined based on chemical composition similarity. Compound 1 (6.9 mg) precipitated as the white powder
during solvent removal from the united fractions 59–69. The pooled fractions 89–92 (80.6 mg) and 95–96 (59.6 mg) gave
compounds 2 (29.8 mg, Rf = 0.5) and 3 (5.8 mg, Rf = 0.53), respectively, after separation by preparative silica gel TLC in a
solvent system consisting of n-hexane, ethyl acetate and formic acid (80:20:1, V/V/V). The structure determination of the iso-
lated prenylated phenols were based on their UV, MS, and 1
H and 13
C NMR spectra. MTT assay was employed to assess their
cytotoxicity against human tumor cell lines (HeLa, HT-29
, HCT-116
, LS174) derived from the cervix adenocarcinoma and colorec-
tal carcinomas, as well as human fetal lung fibroblasts (MRC-5). cis-Diamminedichloroplatinum (CDDP) was used as a control
substance. The activity is expressed as the IC 50 value, i.e. the concentration of the tested substance that led to 50% inhibition
of survival, compared to the vehicle-treated control group. The statistical analysis was performed using the Student’s t-test.
RESULTS:
Based on a comparison of recorded spectral data and literature data, compound 1 was identified as 5,7-dihydroxy-8-gera-
nylflavanone, compound 2 as 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl, and compound 3 as 2-carboxy-3-hydroxy-4-pre-
nyl-5-metoxybibenzyl (amorfrutin A). All tested constituents showed cytotoxicity with the IC50 values in the range 10.55–
166.11 μg/mL. The non-selectivity of compounds 2 and 3 could be observed. On the other hand, compound 1 was selective
and exhibited pronounced activity against the HeLa cell line (IC 50 = 10.55 μg/mL). HT-29 (IC 50 = 122.28 μg/mL) and HCT-116
(IC 50 = 147.09 μg/mL) cancer cell lines, as well as normal cell line MRC-5 (IC50 = 166.11 μg/mL), were less susceptible to the action of compound 1, whereas the survival of LS174 cell line was not affected at all by compound 1 in the tested concen-
tration range. The effectiveness of the control substance CDDP in reducing the survival of the HeLa cancer cell line was the
highest (IC50 = 5.20 μg/mL). It should be emphasized that CDDP, among tested substances, was also the most toxic against
the MRC-5 cell line (IC 50 = 8.28 μg/mL). IC 50 values ranges of compounds 2 and 3 were 31.97–56.29 μg/mL and 35.67–53.21
μg/mL, respectively.
CONCLUSIONS:
5,7-Dihydroxy-8-geranylflavanone, the constituent of false indigo-bush fruit, exhibited strong and selective activity against
the human cervix adenocarcinoma cell line in the MTT assay, therefore, its cytotoxic potential can be considered significant.
Further studies are needed to fully assess the demonstrated effect.",
publisher = "University Prince of Songkla, Thailand, University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts",
title = "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)",
pages = "126-127",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5104"
}

Samardžić, S., Maksimović, Z., Đorđić, M., Stanojković, T.,& Petković, M.. (2021). Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
University Prince of Songkla, Thailand., 126-127.
https://hdl.handle.net/21.15107/rcub_farfar_5104

Samardžić S, Maksimović Z, Đorđić M, Stanojković T, Petković M. Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts. 2021;:126-127.
https://hdl.handle.net/21.15107/rcub_farfar_5104 .

Samardžić, Stevan, Maksimović, Zoran, Đorđić, Marija, Stanojković, Tatjana, Petković, Miloš, "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts (2021):126-127,
https://hdl.handle.net/21.15107/rcub_farfar_5104 .

TY  - JOUR
AU  - Ilić, Milan
AU  - Samardžić, Stevan
AU  - Kotur-Stevuljević, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3995
AB  - Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents
VL  - 25
IS  - 20
SP  - 6283
EP  - 6294
DO  - 10.26355/eurrev_202110_26998
ER  - 

@article{
author = "Ilić, Milan and Samardžić, Stevan and Kotur-Stevuljević, Jelena and Ušjak, Dušan and Milenković, Marina and Kovačević, Nada and Drobac, Milica",
year = "2021",
abstract = "Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents",
volume = "25",
number = "20",
pages = "6283-6294",
doi = "10.26355/eurrev_202110_26998"
}

Ilić, M., Samardžić, S., Kotur-Stevuljević, J., Ušjak, D., Milenković, M., Kovačević, N.,& Drobac, M.. (2021). Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 25(20), 6283-6294.
https://doi.org/10.26355/eurrev_202110_26998

Ilić M, Samardžić S, Kotur-Stevuljević J, Ušjak D, Milenković M, Kovačević N, Drobac M. Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences. 2021;25(20):6283-6294.
doi:10.26355/eurrev_202110_26998 .

Ilić, Milan, Samardžić, Stevan, Kotur-Stevuljević, Jelena, Ušjak, Dušan, Milenković, Marina, Kovačević, Nada, Drobac, Milica, "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents" in European Review for Medical and Pharmacological Sciences, 25, no. 20 (2021):6283-6294,
https://doi.org/10.26355/eurrev_202110_26998 . .