TY  - JOUR
AU  - Filipić, Brankica
AU  - Rapajić-Moran, Ivana
AU  - Nikolić, Ines
AU  - Oljačić, Slavica
AU  - Mandić, Aljoša
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5580
AB  - Human papillomaviruses (HPV) are the most common sexually transmitted pathogens
worldwide, leading to infections with a wide range of clinical manifestations: from benign
conditions to different types of cancer in women and men as well. Cervical cancer is highly
correlated with persistent high-risk-HPV (HR-HPV) infection, which is the key factor in
emergence of 99.99% of cervical cancer cases. The most effective way to prevent HPV-related
cancers is vaccination. There are three available prophylactic HPV vaccines: bivalent,
quadrivalent and nonavalent. The nonavalent vaccine is gradually replacing other HPV vaccines
in most countries and can be given from year 9, but it is commonly routinely implemented at the
age of 11 to 12. The World Health Organization has recognised cervical cancer as a global threat
and has announced the so-called 90-70-90 strategy to reduce and even eliminate cervical cancer.
This strategy implies that 90% of girls should be vaccinated by the age of 15, 70% of women
should be screened for cervical cancer, and 90% of women diagnosed with cervical disease should
receive adequate treatment. Although different treatment options are available: surgery, radiation
therapy, chemotherapy, and advanced target therapy using monoclonal antibodies, great efforts
are needed to achieve the goals set by the World Health Organization to eliminate cervical cancer.
AB  - Humani papilomavirusi (HPV) su među najčešćim uzročnicima seksualno prenosivih patogena i mogu dovesti do različitih kliničkih manifestacija: od benignih stanja do različitih vrsta karcinoma kod žena, ali i muškaraca. Najčešći HPV-posredovan karcinom je karcinom grlića materice koji je u preko 99,99% slučajeva posledica infekcije. Najefikasniji način da se spreči razvoj perzistentne HR-HPV infekcije je vakcinacija. Dostupne su tri profilaktičke vakcine: dvovalentna, kvadrivalentna i devetovalentna. Devetovalentna vakcina pruža najširu zaštitu jer sadrži devet onkogenih HPV genotipova i postepeno zamenjuje ostale vakcine u svim zemljama. Sa vakcinacijom se može krenuti od 9. godine, ali se najčešće rutinski sprovodi kod dečaka i devojčica u uzrastu od 11 do 12 godina. Svetska zdravstvena organizacija je prepoznala karcinom grlića materice kao globalni problem i uvela takozvanu 90-70-90 strategiju u cilju smanjenja stope, pa čak i eliminacije karcinoma grlića materice. Ova strategija podrazumeva da 90% devojčica bude potpuno vakcinisano do 15. godine, 70% žena pristupi redovnom ginekološkom pregledu do 35. godine i ponovo do 45. godine i 90% žena sa promenama na grliću materice primi adekvatnu terapiju. Iako su dostupne različite terapije poput hirurškog tretmana, radioterapije, hemioterapije i ciljane terapije monoklonskim antitelima, i dalje su potrebni veliki napori da bi se dostigli ciljevi Svetske zdravstvene organizacije.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Human papillomaviruses and cervical cancer from the perspective of the World Health Organisation initiative for cervical cancer elimination
T1  - Humani papilomavirusi i karcinom grlića materice iz perspektive inicijative Svetske zdravstvene organizacije za eliminaciju karcinoma grlića materice
VL  - 74
IS  - 1
SP  - 56
EP  - 75
DO  - 10.5937/arhfarm74-48226
ER  - 

@article{
author = "Filipić, Brankica and Rapajić-Moran, Ivana and Nikolić, Ines and Oljačić, Slavica and Mandić, Aljoša",
year = "2024",
abstract = "Human papillomaviruses (HPV) are the most common sexually transmitted pathogens
worldwide, leading to infections with a wide range of clinical manifestations: from benign
conditions to different types of cancer in women and men as well. Cervical cancer is highly
correlated with persistent high-risk-HPV (HR-HPV) infection, which is the key factor in
emergence of 99.99% of cervical cancer cases. The most effective way to prevent HPV-related
cancers is vaccination. There are three available prophylactic HPV vaccines: bivalent,
quadrivalent and nonavalent. The nonavalent vaccine is gradually replacing other HPV vaccines
in most countries and can be given from year 9, but it is commonly routinely implemented at the
age of 11 to 12. The World Health Organization has recognised cervical cancer as a global threat
and has announced the so-called 90-70-90 strategy to reduce and even eliminate cervical cancer.
This strategy implies that 90% of girls should be vaccinated by the age of 15, 70% of women
should be screened for cervical cancer, and 90% of women diagnosed with cervical disease should
receive adequate treatment. Although different treatment options are available: surgery, radiation
therapy, chemotherapy, and advanced target therapy using monoclonal antibodies, great efforts
are needed to achieve the goals set by the World Health Organization to eliminate cervical cancer., Humani papilomavirusi (HPV) su među najčešćim uzročnicima seksualno prenosivih patogena i mogu dovesti do različitih kliničkih manifestacija: od benignih stanja do različitih vrsta karcinoma kod žena, ali i muškaraca. Najčešći HPV-posredovan karcinom je karcinom grlića materice koji je u preko 99,99% slučajeva posledica infekcije. Najefikasniji način da se spreči razvoj perzistentne HR-HPV infekcije je vakcinacija. Dostupne su tri profilaktičke vakcine: dvovalentna, kvadrivalentna i devetovalentna. Devetovalentna vakcina pruža najširu zaštitu jer sadrži devet onkogenih HPV genotipova i postepeno zamenjuje ostale vakcine u svim zemljama. Sa vakcinacijom se može krenuti od 9. godine, ali se najčešće rutinski sprovodi kod dečaka i devojčica u uzrastu od 11 do 12 godina. Svetska zdravstvena organizacija je prepoznala karcinom grlića materice kao globalni problem i uvela takozvanu 90-70-90 strategiju u cilju smanjenja stope, pa čak i eliminacije karcinoma grlića materice. Ova strategija podrazumeva da 90% devojčica bude potpuno vakcinisano do 15. godine, 70% žena pristupi redovnom ginekološkom pregledu do 35. godine i ponovo do 45. godine i 90% žena sa promenama na grliću materice primi adekvatnu terapiju. Iako su dostupne različite terapije poput hirurškog tretmana, radioterapije, hemioterapije i ciljane terapije monoklonskim antitelima, i dalje su potrebni veliki napori da bi se dostigli ciljevi Svetske zdravstvene organizacije.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Human papillomaviruses and cervical cancer from the perspective of the World Health Organisation initiative for cervical cancer elimination, Humani papilomavirusi i karcinom grlića materice iz perspektive inicijative Svetske zdravstvene organizacije za eliminaciju karcinoma grlića materice",
volume = "74",
number = "1",
pages = "56-75",
doi = "10.5937/arhfarm74-48226"
}

Filipić, B., Rapajić-Moran, I., Nikolić, I., Oljačić, S.,& Mandić, A.. (2024). Human papillomaviruses and cervical cancer from the perspective of the World Health Organisation initiative for cervical cancer elimination. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 74(1), 56-75.
https://doi.org/10.5937/arhfarm74-48226

Filipić B, Rapajić-Moran I, Nikolić I, Oljačić S, Mandić A. Human papillomaviruses and cervical cancer from the perspective of the World Health Organisation initiative for cervical cancer elimination. in Arhiv za farmaciju. 2024;74(1):56-75.
doi:10.5937/arhfarm74-48226 .

Filipić, Brankica, Rapajić-Moran, Ivana, Nikolić, Ines, Oljačić, Slavica, Mandić, Aljoša, "Human papillomaviruses and cervical cancer from the perspective of the World Health Organisation initiative for cervical cancer elimination" in Arhiv za farmaciju, 74, no. 1 (2024):56-75,
https://doi.org/10.5937/arhfarm74-48226 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Ušjak, Dušan
AU  - Hrast Rambaher, Martina
AU  - Oljačić, Slavica
AU  - Milenković, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5574
AB  - Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.
PB  - Frontiers Media SA
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Evaluation of novel compounds as anti-bacterial or anti-virulence agents
VL  - 14
DO  - 10.3389/fcimb.2024.1370062
ER  - 

@article{
author = "Filipić, Brankica and Ušjak, Dušan and Hrast Rambaher, Martina and Oljačić, Slavica and Milenković, Marina",
year = "2024",
abstract = "Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Evaluation of novel compounds as anti-bacterial or anti-virulence agents",
volume = "14",
doi = "10.3389/fcimb.2024.1370062"
}

Filipić, B., Ušjak, D., Hrast Rambaher, M., Oljačić, S.,& Milenković, M.. (2024). Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology
Frontiers Media SA., 14.
https://doi.org/10.3389/fcimb.2024.1370062

Filipić B, Ušjak D, Hrast Rambaher M, Oljačić S, Milenković M. Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology. 2024;14.
doi:10.3389/fcimb.2024.1370062 .

Filipić, Brankica, Ušjak, Dušan, Hrast Rambaher, Martina, Oljačić, Slavica, Milenković, Marina, "Evaluation of novel compounds as anti-bacterial or anti-virulence agents" in Frontiers in Cellular and Infection Microbiology, 14 (2024),
https://doi.org/10.3389/fcimb.2024.1370062 . .

TY  - CONF
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5697
AB  - Vakcinacija je dokazano najefikasnija mera za prevenciju i kontrolu infektivnih bolesti, i zahvaljujući vakcinaciji, godišnje se spreči 3,5-5 miliona smrtnih ishoda širom sveta od bolesti poput difterije, teta- nusa, pertusisa, influence i morbila. Dostupne su vakcine za prevenciju oko 20 životno-ugrožavajućih bolesti, koje pomažu ljudima svih doba da žive duži i zdraviji život (1). Zapravo, vakcinacija predstavlja jednu od najuspešnijih i ekonosmki najisplativijih mera prevencije svih vremena.
Povećanje obuhvata vakcinacije je globalni imperativ, gde primarna zdravstvena zaštita ima ključnu ulogu u prioritizaciji preventivnih mera sa ciljem da se smanji stopa obolevanja i zdravstveni sistem učini efikasnijim i dostupnijim. Pored toga, veliku pretnju globalnom zdravlju predstavlja antimikrob- na rezistencija i pojava multi-razistentnih patogena čime se dovodi u pitanje efikasnost trenutno dos- tupnih antibiotika. Vakcinacija doprinosi da se smanji potreba za antibioticima, prevencijom infek- tivnih bolesti, usled čega vakcinacija predstavlja i esencijalnu kariku globalne strategije u borbi protiv antimikrobne rezistencije (2).
Uprkos dokazanom i potvrđenom značaju vakcinacije, neki roditelji odbijaju da vakcinišu svoju decu, usled porasta fenomena “anti-vakserskih” pokreta koji plasiraju lažne informacije povezane sa vak- cinacijom. Zabrinutost i/ili pogrešna informisanost povezana sa imunizacijom, mogu dovesti do specifičnih ponašanja pojedinaca koja za posledicu imaju odlaganje, ili čak odbijanje vakcinacije, što može dovesti do smanjenog obuhvata vakcinacije, gubitka kolektivnog imuniteta i ponovne pojave određenih bolesti (3).
Kampanje za promociju vakcinacije, najčešće su vođene od strane lekara, pedijatara i medicinskih se- stara. Međutim, sa ciljem da se poveća obuhvat vakcinacijom, u promociji vakcinacije sve veći značaj dobijaju farmaceuti u apotekama i farmaceutski tehničari kao i studenti farmacije (4). Nivo u kojem su farmaceuti uključeni u promociju vakcinacije zavisi od regulatornih tela, političkih i socijalnih razlika koje variraju između zemalja. Tako na primer, u Sjedinjenim Američkim Državama (SAD) farmaceuti u apotekama su uključeni u imunizaciju (aplikovanje vakcina) od 1984. godine, a kasnije je njihova uloga dalje ojačana 2009. godine u svih 50 zemalja u SAD (5). Slični programi su implementirani i u Francuskoj i Portugalu. Farmaceuti mogu imati tri osnovne uloge u promociji vakcinacije: 1) farma- ceuti kao facilitatori (pružaju prostor drugima, najčešće lekarima da sprovedu imunizaciju u apoteci), 2) farmaceuti kao edukatori koji imaju važnu ulogu u motivaciji pacijenata da pristupe vakcinaciji, 3) farmaceuti koji aplikuju vakcine (vakcinišu pacijente) (6). Sa COVID-19 pandemijom, Centar za kontrolu i prevenciju bolesti ukazao je na esencijalnu ulogu farmaceuta u odgovoru na pandemiju. Kao posledica toga, standardne operativne procedure su proširene tako da su omogućavale farma- ceutima u pojedinim zemljama da aplikuju COVID-19 vakcine. Od aprila 2022. godine, farmaceuti su aplikovali više od 240 miliona doza COVID-19 vakcina (6).
S obzirom da na našim prostorima, vakcinacija u apotekama još uvek nije zaživela, farmaceuti ima- ju važnu ulogu u prevazilaženju barijera povezanih sa vakcinacijom kroz edukaciju i pružanjem in- formacija zasnovanim na dokazima kako bi se prevazišla pogrešno stečena uverenja kod pacijenata povezana sa vakcinacijom (2). Pristupačnost i rasprostranjenost apoteka, najčešće čini farmaceute na- jdostupnijim zdravstvenim profesionalcima, što daje farmaceutima priliku da promovišu vakcinaciju i edukuju svoje pacijente (2). U proseku, 58% građana Evropske Unije može pristupiti najbližoj apo- teci petominutnom šetnjom, a 98% građana tridesetominutnom šetnjom. Farmaceuti imaju značajnu ulogu u pružanju informacija i saveta o načinu imunizacije (broj doza i razmak između doza) pacijen- tima i opštoj javnosti. Tu se ubraja raspored imunizacije u dečjoj populaciji, što je od posebnog znača- ja, jer su roditelji i trudnice redovni posetioci lokalnih apoteka. Farmaceuti takođe mogu dati savet o obaveznim vakcinama prilikom putovanja u različite delove sveta. Zahvaljujući rigoroznoj edukaciji farmaceuta, farmaceuti predstavljaju jedan od stubova primarne zdravstvene zaštite, često imaju os- tvarenu dugoročnu vezu sa svojim pacijentima, i sve to čini farmaceute neprocenjivim resursom za pružanje informacija zasnovanih na dokazima vezano za vakcinaciju, kao i da informišu pacijente o benefitima i rizicima vakcinacije, pri čemu je važno da informacije koje pružaju budu komplementa- rne sa informacijama koje daju lekari i drugi zdravstveni profesionalci (7). Farmaceuti kao prva tačka kontakta pacijenta sa zdravstvenim sistemom, a najčešće i kao poslednji zdravstveni profesionalci sa kojima se pacijent susretne prilikom preuzimanja leka, su u idealnoj poziciji da daju objašnjenje kako vakcine deluju, na koji način pružaju zaštitu, kakva je efikasnost i bezbednost vakcina i na taj način razbiju mitove i dezinformacije u zajednici unapređujući obuhvat vakcinacije (7).
Može se zaključiti da se poslednjih godina sve više prepoznaje značaj i uloga farmaceuta u promociji vakcinacije.
PB  - Farmaceutsko društvo Republike Srpske u saradnji sa Farmaceutskom komorom Republike Srpske
C3  - Zbornik radova, XXVI Simpozijum farmaceuta Republike Srpske s međunarodnim učešćem, 06.04.2024. Banja Luka
T1  - Uloga farmaceuta u promociji vakcine
SP  - 5
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5697
ER  - 

@conference{
author = "Filipić, Brankica",
year = "2024",
abstract = "Vakcinacija je dokazano najefikasnija mera za prevenciju i kontrolu infektivnih bolesti, i zahvaljujući vakcinaciji, godišnje se spreči 3,5-5 miliona smrtnih ishoda širom sveta od bolesti poput difterije, teta- nusa, pertusisa, influence i morbila. Dostupne su vakcine za prevenciju oko 20 životno-ugrožavajućih bolesti, koje pomažu ljudima svih doba da žive duži i zdraviji život (1). Zapravo, vakcinacija predstavlja jednu od najuspešnijih i ekonosmki najisplativijih mera prevencije svih vremena.
Povećanje obuhvata vakcinacije je globalni imperativ, gde primarna zdravstvena zaštita ima ključnu ulogu u prioritizaciji preventivnih mera sa ciljem da se smanji stopa obolevanja i zdravstveni sistem učini efikasnijim i dostupnijim. Pored toga, veliku pretnju globalnom zdravlju predstavlja antimikrob- na rezistencija i pojava multi-razistentnih patogena čime se dovodi u pitanje efikasnost trenutno dos- tupnih antibiotika. Vakcinacija doprinosi da se smanji potreba za antibioticima, prevencijom infek- tivnih bolesti, usled čega vakcinacija predstavlja i esencijalnu kariku globalne strategije u borbi protiv antimikrobne rezistencije (2).
Uprkos dokazanom i potvrđenom značaju vakcinacije, neki roditelji odbijaju da vakcinišu svoju decu, usled porasta fenomena “anti-vakserskih” pokreta koji plasiraju lažne informacije povezane sa vak- cinacijom. Zabrinutost i/ili pogrešna informisanost povezana sa imunizacijom, mogu dovesti do specifičnih ponašanja pojedinaca koja za posledicu imaju odlaganje, ili čak odbijanje vakcinacije, što može dovesti do smanjenog obuhvata vakcinacije, gubitka kolektivnog imuniteta i ponovne pojave određenih bolesti (3).
Kampanje za promociju vakcinacije, najčešće su vođene od strane lekara, pedijatara i medicinskih se- stara. Međutim, sa ciljem da se poveća obuhvat vakcinacijom, u promociji vakcinacije sve veći značaj dobijaju farmaceuti u apotekama i farmaceutski tehničari kao i studenti farmacije (4). Nivo u kojem su farmaceuti uključeni u promociju vakcinacije zavisi od regulatornih tela, političkih i socijalnih razlika koje variraju između zemalja. Tako na primer, u Sjedinjenim Američkim Državama (SAD) farmaceuti u apotekama su uključeni u imunizaciju (aplikovanje vakcina) od 1984. godine, a kasnije je njihova uloga dalje ojačana 2009. godine u svih 50 zemalja u SAD (5). Slični programi su implementirani i u Francuskoj i Portugalu. Farmaceuti mogu imati tri osnovne uloge u promociji vakcinacije: 1) farma- ceuti kao facilitatori (pružaju prostor drugima, najčešće lekarima da sprovedu imunizaciju u apoteci), 2) farmaceuti kao edukatori koji imaju važnu ulogu u motivaciji pacijenata da pristupe vakcinaciji, 3) farmaceuti koji aplikuju vakcine (vakcinišu pacijente) (6). Sa COVID-19 pandemijom, Centar za kontrolu i prevenciju bolesti ukazao je na esencijalnu ulogu farmaceuta u odgovoru na pandemiju. Kao posledica toga, standardne operativne procedure su proširene tako da su omogućavale farma- ceutima u pojedinim zemljama da aplikuju COVID-19 vakcine. Od aprila 2022. godine, farmaceuti su aplikovali više od 240 miliona doza COVID-19 vakcina (6).
S obzirom da na našim prostorima, vakcinacija u apotekama još uvek nije zaživela, farmaceuti ima- ju važnu ulogu u prevazilaženju barijera povezanih sa vakcinacijom kroz edukaciju i pružanjem in- formacija zasnovanim na dokazima kako bi se prevazišla pogrešno stečena uverenja kod pacijenata povezana sa vakcinacijom (2). Pristupačnost i rasprostranjenost apoteka, najčešće čini farmaceute na- jdostupnijim zdravstvenim profesionalcima, što daje farmaceutima priliku da promovišu vakcinaciju i edukuju svoje pacijente (2). U proseku, 58% građana Evropske Unije može pristupiti najbližoj apo- teci petominutnom šetnjom, a 98% građana tridesetominutnom šetnjom. Farmaceuti imaju značajnu ulogu u pružanju informacija i saveta o načinu imunizacije (broj doza i razmak između doza) pacijen- tima i opštoj javnosti. Tu se ubraja raspored imunizacije u dečjoj populaciji, što je od posebnog znača- ja, jer su roditelji i trudnice redovni posetioci lokalnih apoteka. Farmaceuti takođe mogu dati savet o obaveznim vakcinama prilikom putovanja u različite delove sveta. Zahvaljujući rigoroznoj edukaciji farmaceuta, farmaceuti predstavljaju jedan od stubova primarne zdravstvene zaštite, često imaju os- tvarenu dugoročnu vezu sa svojim pacijentima, i sve to čini farmaceute neprocenjivim resursom za pružanje informacija zasnovanih na dokazima vezano za vakcinaciju, kao i da informišu pacijente o benefitima i rizicima vakcinacije, pri čemu je važno da informacije koje pružaju budu komplementa- rne sa informacijama koje daju lekari i drugi zdravstveni profesionalci (7). Farmaceuti kao prva tačka kontakta pacijenta sa zdravstvenim sistemom, a najčešće i kao poslednji zdravstveni profesionalci sa kojima se pacijent susretne prilikom preuzimanja leka, su u idealnoj poziciji da daju objašnjenje kako vakcine deluju, na koji način pružaju zaštitu, kakva je efikasnost i bezbednost vakcina i na taj način razbiju mitove i dezinformacije u zajednici unapređujući obuhvat vakcinacije (7).
Može se zaključiti da se poslednjih godina sve više prepoznaje značaj i uloga farmaceuta u promociji vakcinacije.",
publisher = "Farmaceutsko društvo Republike Srpske u saradnji sa Farmaceutskom komorom Republike Srpske",
journal = "Zbornik radova, XXVI Simpozijum farmaceuta Republike Srpske s međunarodnim učešćem, 06.04.2024. Banja Luka",
title = "Uloga farmaceuta u promociji vakcine",
pages = "5-6",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5697"
}

Filipić, B.. (2024). Uloga farmaceuta u promociji vakcine. in Zbornik radova, XXVI Simpozijum farmaceuta Republike Srpske s međunarodnim učešćem, 06.04.2024. Banja Luka
Farmaceutsko društvo Republike Srpske u saradnji sa Farmaceutskom komorom Republike Srpske., 5-6.
https://hdl.handle.net/21.15107/rcub_farfar_5697

Filipić B. Uloga farmaceuta u promociji vakcine. in Zbornik radova, XXVI Simpozijum farmaceuta Republike Srpske s međunarodnim učešćem, 06.04.2024. Banja Luka. 2024;:5-6.
https://hdl.handle.net/21.15107/rcub_farfar_5697 .

Filipić, Brankica, "Uloga farmaceuta u promociji vakcine" in Zbornik radova, XXVI Simpozijum farmaceuta Republike Srpske s međunarodnim učešćem, 06.04.2024. Banja Luka (2024):5-6,
https://hdl.handle.net/21.15107/rcub_farfar_5697 .

TY  - CONF
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Stević, Tatjana
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5689
AB  - Antimicrobial resistance (AMR) threatens to in- crease its mortality rate to 10 million deaths per year by 2050, and Acinetobacter bauman- nii stands out with its insensitivity to almost all available therapeutic options. The risk factors for the acquisition of A. baumannii infections have become more frequent during the coronavirus disease 2019 (COVID-19) pandemic, consequent- ly leading to increased AMR. Potential solutions may be explored among naturally derived prod- ucts, where essential oils (EOs) stand out with a broad spectrum of antimicrobial activity, com- plex chemical composition, and non-specific mechanisms of action postponing AMR devel- opment. However, their volatile nature may af- fect stability during shelf-life. Thus, we aimed to develop stable and effective microencapsulat- ed systems of essential oils (EOs) derived from two species from Lamiaceae family, Origanum heracleoticum L. (oregano) and Thymus vulgar- is L. (thyme). Microencapsulated complexes of oregano and thyme EOs (OEOC and TEOC) were prepared with hydroxypropyl-β-cyclodextrin as a carrier, and freeze-dried. Fourier-transform infrared spectroscopy verified the formation of inclusion complexes, while thermal stability was confirmed by differential scanning calorimetry. The microdilution broth assay revealed higher antimicrobial activity of the OEOC sample com- pared to TEOC against 64 A. baumannii isolates recovered from COVID-19 patients admitted to intensive care units (MIC values 0.4–1.6 mg/mL for OEOC, and ≥ 1.76 mg/mL for TEOC). Sub-in- hibitory concentrations of microencapsulated EOs significantly decreased the biofilm forma- tion of four A. baumannii isolates, representatives of a group of isolates based on the genetic pat- tern (Isolates 1, 2, 39, and 54). Concerning Isolate 2 (representing 60 of the total 64 isolates), the re- duction was achieved for more than 50% by both samples. Contrary to antimicrobial potential, TEOC displayed slightly better antioxidant activ- ity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. These results highlight the potential of mi- croencapsulated oregano and thyme EOs in the treatment of infections caused by A. baumannii.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts
T1  - Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii
SP  - 179
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5689
ER  - 

@conference{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Stević, Tatjana and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Antimicrobial resistance (AMR) threatens to in- crease its mortality rate to 10 million deaths per year by 2050, and Acinetobacter bauman- nii stands out with its insensitivity to almost all available therapeutic options. The risk factors for the acquisition of A. baumannii infections have become more frequent during the coronavirus disease 2019 (COVID-19) pandemic, consequent- ly leading to increased AMR. Potential solutions may be explored among naturally derived prod- ucts, where essential oils (EOs) stand out with a broad spectrum of antimicrobial activity, com- plex chemical composition, and non-specific mechanisms of action postponing AMR devel- opment. However, their volatile nature may af- fect stability during shelf-life. Thus, we aimed to develop stable and effective microencapsulat- ed systems of essential oils (EOs) derived from two species from Lamiaceae family, Origanum heracleoticum L. (oregano) and Thymus vulgar- is L. (thyme). Microencapsulated complexes of oregano and thyme EOs (OEOC and TEOC) were prepared with hydroxypropyl-β-cyclodextrin as a carrier, and freeze-dried. Fourier-transform infrared spectroscopy verified the formation of inclusion complexes, while thermal stability was confirmed by differential scanning calorimetry. The microdilution broth assay revealed higher antimicrobial activity of the OEOC sample com- pared to TEOC against 64 A. baumannii isolates recovered from COVID-19 patients admitted to intensive care units (MIC values 0.4–1.6 mg/mL for OEOC, and ≥ 1.76 mg/mL for TEOC). Sub-in- hibitory concentrations of microencapsulated EOs significantly decreased the biofilm forma- tion of four A. baumannii isolates, representatives of a group of isolates based on the genetic pat- tern (Isolates 1, 2, 39, and 54). Concerning Isolate 2 (representing 60 of the total 64 isolates), the re- duction was achieved for more than 50% by both samples. Contrary to antimicrobial potential, TEOC displayed slightly better antioxidant activ- ity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. These results highlight the potential of mi- croencapsulated oregano and thyme EOs in the treatment of infections caused by A. baumannii.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts",
title = "Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii",
pages = "179-179",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5689"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Stević, T., Bigović, D., Šavikin, K.,& Filipić, B.. (2024). Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii. in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts
Serbian Society for Microbiology., 179-179.
https://hdl.handle.net/21.15107/rcub_farfar_5689

Kuzmanović Nedeljković S, Ćujić Nikolić N, Stević T, Bigović D, Šavikin K, Filipić B. Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii. in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts. 2024;:179-179.
https://hdl.handle.net/21.15107/rcub_farfar_5689 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Stević, Tatjana, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii" in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts (2024):179-179,
https://hdl.handle.net/21.15107/rcub_farfar_5689 .

TY  - JOUR
AU  - Dyląg, Mariusz
AU  - Filipić, Brankica
AU  - Augustyniak, Daria
AU  - Milenković, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5686
PB  - Frontiers Media SA
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Editorial: Research implications on microbial virulence factors, resistance, and new therapeutic strategies in the context of future infectious disease therapies
VL  - 14
DO  - 10.3389/fcimb.2024.1406119
ER  - 

@article{
author = "Dyląg, Mariusz and Filipić, Brankica and Augustyniak, Daria and Milenković, Marina",
year = "2024",
publisher = "Frontiers Media SA",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Editorial: Research implications on microbial virulence factors, resistance, and new therapeutic strategies in the context of future infectious disease therapies",
volume = "14",
doi = "10.3389/fcimb.2024.1406119"
}

Dyląg, M., Filipić, B., Augustyniak, D.,& Milenković, M.. (2024). Editorial: Research implications on microbial virulence factors, resistance, and new therapeutic strategies in the context of future infectious disease therapies. in Frontiers in Cellular and Infection Microbiology
Frontiers Media SA., 14.
https://doi.org/10.3389/fcimb.2024.1406119

Dyląg M, Filipić B, Augustyniak D, Milenković M. Editorial: Research implications on microbial virulence factors, resistance, and new therapeutic strategies in the context of future infectious disease therapies. in Frontiers in Cellular and Infection Microbiology. 2024;14.
doi:10.3389/fcimb.2024.1406119 .

Dyląg, Mariusz, Filipić, Brankica, Augustyniak, Daria, Milenković, Marina, "Editorial: Research implications on microbial virulence factors, resistance, and new therapeutic strategies in the context of future infectious disease therapies" in Frontiers in Cellular and Infection Microbiology, 14 (2024),
https://doi.org/10.3389/fcimb.2024.1406119 . .

TY  - JOUR
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Radan, Milica
AU  - Milivojević, Dušan
AU  - Stević, Tatjana
AU  - Pljevljakušić, Dejan
AU  - Nikodinović-Runić, Jasmina
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5642
AB  - Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii
VL  - 216
DO  - 10.1016/j.indcrop.2024.118762
ER  - 

@article{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Radan, Milica and Milivojević, Dušan and Stević, Tatjana and Pljevljakušić, Dejan and Nikodinović-Runić, Jasmina and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii",
volume = "216",
doi = "10.1016/j.indcrop.2024.118762"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Radan, M., Milivojević, D., Stević, T., Pljevljakušić, D., Nikodinović-Runić, J., Bigović, D., Šavikin, K.,& Filipić, B.. (2024). Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products
Elsevier B.V.., 216.
https://doi.org/10.1016/j.indcrop.2024.118762

Kuzmanović Nedeljković S, Ćujić Nikolić N, Radan M, Milivojević D, Stević T, Pljevljakušić D, Nikodinović-Runić J, Bigović D, Šavikin K, Filipić B. Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products. 2024;216.
doi:10.1016/j.indcrop.2024.118762 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Radan, Milica, Milivojević, Dušan, Stević, Tatjana, Pljevljakušić, Dejan, Nikodinović-Runić, Jasmina, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii" in Industrial Crops and Products, 216 (2024),
https://doi.org/10.1016/j.indcrop.2024.118762 . .

TY  - JOUR
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Antonić, Tamara
AU  - Bogavac-Stanojević, Nataša
AU  - Novović, Katarina
AU  - Spasojević-Kalimanovska, Vesna
AU  - Filipić, Brankica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5421
AB  - Background: The pre-analytical (PA) phase is the most vulnerable phase of laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage. The aim of this study was to examine if different anticoagulants, storage conditions, and freeze-thaw cycles (FTCs) influence the concentrations of basic biochemical parameters. In parallel, the presence and the effect of sample microbiological contamination during routine laboratory work were examined.

Methods: Two plasma pools (EDTA, and sodium-fluoride/potassium oxalate plasma (NaF)) were stored at +4C˚/-20˚C. Total cholesterol (TC), glucose, triglycerides (TG), urea, total protein (TP), and albumin concentrations were measured using Ilab 300+. Sample microbiological contamination was determined by 16S rRNA sequence analysis. The experiment encompassed a 5 day-period: Day 1–fresh sample, Day 2–1st FTC, Day 3–2nd FTC, Day 4–3rd FTC, Day 5–4th FTC. The appearance of bacteria in two consecutive samples was the experiment's endpoint.

Results: During 4 FTCs there were no changes in plasma urea concentrations. Glucose was stable in EDTA+4˚C and NaF- 20˚C until the 3rd FTC (P=0.008, P=0.042, respectively). Changes in protein concentrations followed the zig-zag pattern. TG concentrations changed significantly in the EDTA-20˚C sample after 1st and 4th FTCs (P=0.022, P=0.010, respectively). In NaF samples no contamination was observed during 4 FTCs.

 

Conclusions: Urea and glucose concentrations were robust. Changes in lipid and protein concentrations after FTCs follow complex patterns. Bacterial growth was not observed in NaF plasma samples. This can promote NaF use in analytical procedures in which microbiological contamination affects the quality of analysis.
AB  - Uvod: Preanaliti~ka (PA) faza je slo`en proces koji ~ine:
prikupljanje, rukovanje, transport i skladi{tenje uzoraka, i
predstavlja najzna~ajniji izvor laboratorijskih gre{aka. Cilj
ovog istra`ivanja je bio da se ispita stabilnost osnovnih
biohemijskih parametara u zavisnosti od uslova skladi{tenja
uzoraka i broja ciklusa zamrzavanja-odmrzavanja (FTC).
Pored toga, ispitivano je prisustvo bakterijske kontaminacije
uzoraka tokom rutinskog laboratorijskog rada.
Metode: Dva »pool«-a plazme (etilendiaminotetrasir}etna
kiselina (EDTA) i natrijum-fluorid/kalijum oksalat (NaF)) su
skladi{tena na +4 ˚C/-20 ˚C. Koncentracije ukupnog
holesterola (TC), glukoze, triglicerida (TG), uree i albumina
su odre|ene kori{}enjem BioSystems reagenasa (holesterol
oksidaza/peroksidaza, glukoza oksidaza/peroksidaza,
glice rol fosfat oksidaza/peroksidaza, ureaza/salicilat, od-
nosno bromkrezol zeleno metodama, sukcesivno) na Ilab
300+ analizatoru. Bakterijska kontaminacija uzoraka je
potvr|ena 16S rRNA sekvencioniranjem. Eksperiment je
sproveden tokom 5 uzastopnih dana: 1. dan – sve` uzorak,
2. dan – 1. FTC, 3. dan –2. FTC, 4. dan – 3. FTC, 5. dan
– 4. FTC. Zavr{nu ta~ku eksperimenta predstavljala je
pojava bakterija u dva uzastopna uzorka.
Rezultati: Tokom 4 FTC koncentracije uree u plazmi se nisu
zna~ajno razlikovale. Koncentracija glukoze je bila stabilna
u EDTA +4 ˚C i NaF -20 ˚C do 3.FTC (P=0,008,
P=0,042, redom). Koncentracije TG su se zna~ajno pro-
menile u uzorku EDTA -20 ˚C nakon 1. i 4. FTC-a (P=0,022, P=0,010, redom). U uzorcima NaF plazme
nije do{lo do bakterijske kontaminacije tokom 4. FTC.
Zaklju~ak: Koncentracije uree i glukoze su bile stabilne
tokom trajanja eksperimenta. Promene u koncentracijama
lipida nakon FTC prate slo`ene obrasce. Rast bakterija nije
prime}en u uzorcima NaF plazme, te upotreba ovog anti-
koagulansa mo`e biti opravdana u analiti~kim proce -
durama podlo`nim uticaju mikrobiolo{ke kontaminacije.
PB  - Journal of Medical Biochemistry
T2  - Journal of Medical Biochemistry
T1  - The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters
T1  - Uticaj različitih uslova čuvanja uzoraka i kontaminacije uzoraka bakterijama na koncentracije rutinskih biohemijskih parametara
VL  - 43
IS  - 3
SP  - 413
EP  - 423
DO  - 10.5937/jomb0-40360
ER  - 

@article{
author = "Gojković, Tamara and Vladimirov, Sandra and Antonić, Tamara and Bogavac-Stanojević, Nataša and Novović, Katarina and Spasojević-Kalimanovska, Vesna and Filipić, Brankica",
year = "2023",
abstract = "Background: The pre-analytical (PA) phase is the most vulnerable phase of laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage. The aim of this study was to examine if different anticoagulants, storage conditions, and freeze-thaw cycles (FTCs) influence the concentrations of basic biochemical parameters. In parallel, the presence and the effect of sample microbiological contamination during routine laboratory work were examined.

Methods: Two plasma pools (EDTA, and sodium-fluoride/potassium oxalate plasma (NaF)) were stored at +4C˚/-20˚C. Total cholesterol (TC), glucose, triglycerides (TG), urea, total protein (TP), and albumin concentrations were measured using Ilab 300+. Sample microbiological contamination was determined by 16S rRNA sequence analysis. The experiment encompassed a 5 day-period: Day 1–fresh sample, Day 2–1st FTC, Day 3–2nd FTC, Day 4–3rd FTC, Day 5–4th FTC. The appearance of bacteria in two consecutive samples was the experiment's endpoint.

Results: During 4 FTCs there were no changes in plasma urea concentrations. Glucose was stable in EDTA+4˚C and NaF- 20˚C until the 3rd FTC (P=0.008, P=0.042, respectively). Changes in protein concentrations followed the zig-zag pattern. TG concentrations changed significantly in the EDTA-20˚C sample after 1st and 4th FTCs (P=0.022, P=0.010, respectively). In NaF samples no contamination was observed during 4 FTCs.

 

Conclusions: Urea and glucose concentrations were robust. Changes in lipid and protein concentrations after FTCs follow complex patterns. Bacterial growth was not observed in NaF plasma samples. This can promote NaF use in analytical procedures in which microbiological contamination affects the quality of analysis., Uvod: Preanaliti~ka (PA) faza je slo`en proces koji ~ine:
prikupljanje, rukovanje, transport i skladi{tenje uzoraka, i
predstavlja najzna~ajniji izvor laboratorijskih gre{aka. Cilj
ovog istra`ivanja je bio da se ispita stabilnost osnovnih
biohemijskih parametara u zavisnosti od uslova skladi{tenja
uzoraka i broja ciklusa zamrzavanja-odmrzavanja (FTC).
Pored toga, ispitivano je prisustvo bakterijske kontaminacije
uzoraka tokom rutinskog laboratorijskog rada.
Metode: Dva »pool«-a plazme (etilendiaminotetrasir}etna
kiselina (EDTA) i natrijum-fluorid/kalijum oksalat (NaF)) su
skladi{tena na +4 ˚C/-20 ˚C. Koncentracije ukupnog
holesterola (TC), glukoze, triglicerida (TG), uree i albumina
su odre|ene kori{}enjem BioSystems reagenasa (holesterol
oksidaza/peroksidaza, glukoza oksidaza/peroksidaza,
glice rol fosfat oksidaza/peroksidaza, ureaza/salicilat, od-
nosno bromkrezol zeleno metodama, sukcesivno) na Ilab
300+ analizatoru. Bakterijska kontaminacija uzoraka je
potvr|ena 16S rRNA sekvencioniranjem. Eksperiment je
sproveden tokom 5 uzastopnih dana: 1. dan – sve` uzorak,
2. dan – 1. FTC, 3. dan –2. FTC, 4. dan – 3. FTC, 5. dan
– 4. FTC. Zavr{nu ta~ku eksperimenta predstavljala je
pojava bakterija u dva uzastopna uzorka.
Rezultati: Tokom 4 FTC koncentracije uree u plazmi se nisu
zna~ajno razlikovale. Koncentracija glukoze je bila stabilna
u EDTA +4 ˚C i NaF -20 ˚C do 3.FTC (P=0,008,
P=0,042, redom). Koncentracije TG su se zna~ajno pro-
menile u uzorku EDTA -20 ˚C nakon 1. i 4. FTC-a (P=0,022, P=0,010, redom). U uzorcima NaF plazme
nije do{lo do bakterijske kontaminacije tokom 4. FTC.
Zaklju~ak: Koncentracije uree i glukoze su bile stabilne
tokom trajanja eksperimenta. Promene u koncentracijama
lipida nakon FTC prate slo`ene obrasce. Rast bakterija nije
prime}en u uzorcima NaF plazme, te upotreba ovog anti-
koagulansa mo`e biti opravdana u analiti~kim proce -
durama podlo`nim uticaju mikrobiolo{ke kontaminacije.",
publisher = "Journal of Medical Biochemistry",
journal = "Journal of Medical Biochemistry",
title = "The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters, Uticaj različitih uslova čuvanja uzoraka i kontaminacije uzoraka bakterijama na koncentracije rutinskih biohemijskih parametara",
volume = "43",
number = "3",
pages = "413-423",
doi = "10.5937/jomb0-40360"
}

Gojković, T., Vladimirov, S., Antonić, T., Bogavac-Stanojević, N., Novović, K., Spasojević-Kalimanovska, V.,& Filipić, B.. (2023). The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters. in Journal of Medical Biochemistry
Journal of Medical Biochemistry., 43(3), 413-423.
https://doi.org/10.5937/jomb0-40360

Gojković T, Vladimirov S, Antonić T, Bogavac-Stanojević N, Novović K, Spasojević-Kalimanovska V, Filipić B. The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters. in Journal of Medical Biochemistry. 2023;43(3):413-423.
doi:10.5937/jomb0-40360 .

Gojković, Tamara, Vladimirov, Sandra, Antonić, Tamara, Bogavac-Stanojević, Nataša, Novović, Katarina, Spasojević-Kalimanovska, Vesna, Filipić, Brankica, "The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters" in Journal of Medical Biochemistry, 43, no. 3 (2023):413-423,
https://doi.org/10.5937/jomb0-40360 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Filipić, Brankica
AU  - Marija Petrović
AU  - Jordan, Olivier
AU  - Savić, Snežana
AU  - Borchard, Gerrit
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5358
AB  - The field of nanotechnology is at the forefront of a scientific revolution, where the term
“nano” transcends mere size and opens the door to enormous possibilities.
In the context of drug development, the selection of a suitable drug delivery system
(corresponding to a certain active pharmaceutical ingredient) is a pivotal decision. Accordingly,
nanosystems have emerged as a promising avenue, offering innovative solutions, and gaining
recognition for addressing healthcare issues.
While these products hold immense promise, they have faced certain complexities in their
translation from the preclinical to the clinical setting, reflected in the lack of proper assessment
protocols for quality and safety aspects and, consequently, an insufficiently defined regulatory
environment. Since the groundbreaking US Food and Drug Administration (FDA) approval of
liposomal doxorubicin in 1995, approximately 80 nanomedicine products have received regulatory
approval so far. Recent attention has gravitated toward lipid-based nanomedicines, particularly in
the development of mRNA vaccines during the COVID-19 pandemic, further highlighting their
significance. However, the relatively modest number of approved nanomedicines compared to the
extensive research efforts raises important questions and underscores areas of uncertainty.
This article provides an overview of the challenges in defining nanomedicines, their
properties, the complexities of regulatory frameworks, and the imperative for standardized
characterization protocols.
AB  - Polje nanotehnologije se nalazi na čelu naučne revolucije, gde se termin "nano" izdiže iznad pukog označavanja veličine, otvarajući vrata novim mogućnostima. U kontekstu razvoja lekova, izbor odgovarajućeg sistema za isporuku / nosača (koji odgovara određenoj aktivnoj supstanci) predstavlja ključnu odluku. U tom kontekstu, nanosistemi već određeno vreme predstavljaju inovativna rešenja. Iako farmaceutski nanosistemi nose ogroman potencijal, suočavaju se sa određenim izazovima u pogledu translacije sa prekliničkog na klinički nivo, što se ogleda u nedostatku odgovarajućih protokola za ispitivanje kvaliteta i bezbednosti i, shodno tome, nedefinisanom regulatornom okruženju. Od revolucionarnog odobrenja liposomalnog doksorubicina od strane Američke agencije za hranu i lekove 1995. godine, pa sve do danas, oko 80 nano formulacija (nanolekova) odobreno je za kliničku primenu. Odnedavno je intenzivnija pažnja usmerena ka nanoformulacijama baziranim na lipidima, što je delom posledica razvoja mRNK vakcina tokom pandemije COVID-19. Međutim, relativno skroman nastup nanolekova na tržištu (u poređenju sa obimnim istraživačkim naporima i finansijskim ulaganjima u ovu oblast) otvara važna pitanja. Ovaj rad pruža pregled izazova u definisanju nanolekova, njihovih svojstava, kompleksnosti regulatornih okvira i imperativa za stvaranje standardizovanih protokola karakterizacije.
PB  - Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - The Landscape of Nanomedicines: An Expert Perspective
T1  - "Pejzažni" prikaz nanolekova - ekspertska perspektiva
VL  - 73
IS  - 5
SP  - 390
EP  - 403
DO  - 10.5937/arhfarm73-46686
ER  - 

@article{
author = "Nikolić, Ines and Filipić, Brankica and Marija Petrović and Jordan, Olivier and Savić, Snežana and Borchard, Gerrit",
year = "2023",
abstract = "The field of nanotechnology is at the forefront of a scientific revolution, where the term
“nano” transcends mere size and opens the door to enormous possibilities.
In the context of drug development, the selection of a suitable drug delivery system
(corresponding to a certain active pharmaceutical ingredient) is a pivotal decision. Accordingly,
nanosystems have emerged as a promising avenue, offering innovative solutions, and gaining
recognition for addressing healthcare issues.
While these products hold immense promise, they have faced certain complexities in their
translation from the preclinical to the clinical setting, reflected in the lack of proper assessment
protocols for quality and safety aspects and, consequently, an insufficiently defined regulatory
environment. Since the groundbreaking US Food and Drug Administration (FDA) approval of
liposomal doxorubicin in 1995, approximately 80 nanomedicine products have received regulatory
approval so far. Recent attention has gravitated toward lipid-based nanomedicines, particularly in
the development of mRNA vaccines during the COVID-19 pandemic, further highlighting their
significance. However, the relatively modest number of approved nanomedicines compared to the
extensive research efforts raises important questions and underscores areas of uncertainty.
This article provides an overview of the challenges in defining nanomedicines, their
properties, the complexities of regulatory frameworks, and the imperative for standardized
characterization protocols., Polje nanotehnologije se nalazi na čelu naučne revolucije, gde se termin "nano" izdiže iznad pukog označavanja veličine, otvarajući vrata novim mogućnostima. U kontekstu razvoja lekova, izbor odgovarajućeg sistema za isporuku / nosača (koji odgovara određenoj aktivnoj supstanci) predstavlja ključnu odluku. U tom kontekstu, nanosistemi već određeno vreme predstavljaju inovativna rešenja. Iako farmaceutski nanosistemi nose ogroman potencijal, suočavaju se sa određenim izazovima u pogledu translacije sa prekliničkog na klinički nivo, što se ogleda u nedostatku odgovarajućih protokola za ispitivanje kvaliteta i bezbednosti i, shodno tome, nedefinisanom regulatornom okruženju. Od revolucionarnog odobrenja liposomalnog doksorubicina od strane Američke agencije za hranu i lekove 1995. godine, pa sve do danas, oko 80 nano formulacija (nanolekova) odobreno je za kliničku primenu. Odnedavno je intenzivnija pažnja usmerena ka nanoformulacijama baziranim na lipidima, što je delom posledica razvoja mRNK vakcina tokom pandemije COVID-19. Međutim, relativno skroman nastup nanolekova na tržištu (u poređenju sa obimnim istraživačkim naporima i finansijskim ulaganjima u ovu oblast) otvara važna pitanja. Ovaj rad pruža pregled izazova u definisanju nanolekova, njihovih svojstava, kompleksnosti regulatornih okvira i imperativa za stvaranje standardizovanih protokola karakterizacije.",
publisher = "Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "The Landscape of Nanomedicines: An Expert Perspective, "Pejzažni" prikaz nanolekova - ekspertska perspektiva",
volume = "73",
number = "5",
pages = "390-403",
doi = "10.5937/arhfarm73-46686"
}

Nikolić, I., Filipić, B., Marija Petrović, Jordan, O., Savić, S.,& Borchard, G.. (2023). The Landscape of Nanomedicines: An Expert Perspective. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije., 73(5), 390-403.
https://doi.org/10.5937/arhfarm73-46686

Nikolić I, Filipić B, Marija Petrović, Jordan O, Savić S, Borchard G. The Landscape of Nanomedicines: An Expert Perspective. in Arhiv za farmaciju. 2023;73(5):390-403.
doi:10.5937/arhfarm73-46686 .

Nikolić, Ines, Filipić, Brankica, Marija Petrović, Jordan, Olivier, Savić, Snežana, Borchard, Gerrit, "The Landscape of Nanomedicines: An Expert Perspective" in Arhiv za farmaciju, 73, no. 5 (2023):390-403,
https://doi.org/10.5937/arhfarm73-46686 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Pantelić, Ivana
AU  - Nikolić, Ines
AU  - Majhen, Dragomira
AU  - Stojić-Vukanić, Zorica
AU  - Savić, Snežana
AU  - Krajišnik, Danina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4960
AB  - Ever since the development of the first vaccine, vaccination has had the great impact on global health, leading to the decrease in the burden of numerous infectious diseases. However, there is a constant need to improve existing vaccines and develop new vaccination strategies and vaccine platforms that induce a broader immune response compared to traditional vaccines. Modern vaccines tend to rely on certain nanotechnology platforms but are still expected to be readily available and easy for large-scale manufacturing and to induce a durable immune response. In this review, we present an overview of the most promising nanoadjuvants and nanoparticulate delivery systems and discuss their benefits from tehchnological and immunological standpoints as well as their objective drawbacks and possible side effects. The presented nano alums, silica and clay nanoparticles, nanoemulsions, adenoviral-vectored systems, adeno-associated viral vectors, vesicular stomatitis viral vectors, lentiviral vectors, virus-like particles (including bacteriophage-based ones) and virosomes indicate that vaccine developers can now choose different adjuvants and/or delivery systems as per the requirement, specific to combatting different infectious diseases.
PB  - MDPI
T2  - Vaccines
T1  - Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines
VL  - 11
IS  - 7
DO  - 10.3390/vaccines11071172
ER  - 

@article{
author = "Filipić, Brankica and Pantelić, Ivana and Nikolić, Ines and Majhen, Dragomira and Stojić-Vukanić, Zorica and Savić, Snežana and Krajišnik, Danina",
year = "2023",
abstract = "Ever since the development of the first vaccine, vaccination has had the great impact on global health, leading to the decrease in the burden of numerous infectious diseases. However, there is a constant need to improve existing vaccines and develop new vaccination strategies and vaccine platforms that induce a broader immune response compared to traditional vaccines. Modern vaccines tend to rely on certain nanotechnology platforms but are still expected to be readily available and easy for large-scale manufacturing and to induce a durable immune response. In this review, we present an overview of the most promising nanoadjuvants and nanoparticulate delivery systems and discuss their benefits from tehchnological and immunological standpoints as well as their objective drawbacks and possible side effects. The presented nano alums, silica and clay nanoparticles, nanoemulsions, adenoviral-vectored systems, adeno-associated viral vectors, vesicular stomatitis viral vectors, lentiviral vectors, virus-like particles (including bacteriophage-based ones) and virosomes indicate that vaccine developers can now choose different adjuvants and/or delivery systems as per the requirement, specific to combatting different infectious diseases.",
publisher = "MDPI",
journal = "Vaccines",
title = "Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines",
volume = "11",
number = "7",
doi = "10.3390/vaccines11071172"
}

Filipić, B., Pantelić, I., Nikolić, I., Majhen, D., Stojić-Vukanić, Z., Savić, S.,& Krajišnik, D.. (2023). Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines. in Vaccines
MDPI., 11(7).
https://doi.org/10.3390/vaccines11071172

Filipić B, Pantelić I, Nikolić I, Majhen D, Stojić-Vukanić Z, Savić S, Krajišnik D. Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines. in Vaccines. 2023;11(7).
doi:10.3390/vaccines11071172 .

Filipić, Brankica, Pantelić, Ivana, Nikolić, Ines, Majhen, Dragomira, Stojić-Vukanić, Zorica, Savić, Snežana, Krajišnik, Danina, "Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines" in Vaccines, 11, no. 7 (2023),
https://doi.org/10.3390/vaccines11071172 . .

TY  - CONF
AU  - Filipić, Brankica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5226
AB  - Human papilloma viruses (HPV) are small non-enveloped DNA viruses that posses
great affinity for epithelial tissue. So far, more than 200 genotypes of HPV have been
identified, of which around 40 types are associated with genital infections in man and
woman. HPV is the most sexually transmited virus in the world leading to the fact that
HPV infection is the major cause of cervical cancer. Besides, HPV is thought to be
responsible for more than 90% of anal cancer, 70% of vaginal and vulvar cancer, 70%
of oropharingeal cancer and 60% of penile cancer [1, 2]. HPV vaccinationn is the most
effective procedure in prevention of HPV-related cancers. Prophylactic 9-valent HPV
vaccine which targets high-risk HPV types is the most preferable one and is available
in many countries worlwide including Balkan region. The main goal of this presentation
is to improve awareness of great importance of HPV vaccination in target popullations.
PB  - Komora magistara farmacije Federacije Bosne i hercegovine
PB  - Farmaceutska komora Republike Srpske
PB  - Farmaceutsko društvo Republike Srpske
C3  - 5th Congress of Pharmacists of Bosnia and Herzegovina with international participation, Abstract book, „Competencies of pharmacists – from drug design to successful disease treatment“
T1  - About the HPV vaccine: how it works and who can get it
SP  - 35
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5226
ER  - 

@conference{
author = "Filipić, Brankica",
year = "2023",
abstract = "Human papilloma viruses (HPV) are small non-enveloped DNA viruses that posses
great affinity for epithelial tissue. So far, more than 200 genotypes of HPV have been
identified, of which around 40 types are associated with genital infections in man and
woman. HPV is the most sexually transmited virus in the world leading to the fact that
HPV infection is the major cause of cervical cancer. Besides, HPV is thought to be
responsible for more than 90% of anal cancer, 70% of vaginal and vulvar cancer, 70%
of oropharingeal cancer and 60% of penile cancer [1, 2]. HPV vaccinationn is the most
effective procedure in prevention of HPV-related cancers. Prophylactic 9-valent HPV
vaccine which targets high-risk HPV types is the most preferable one and is available
in many countries worlwide including Balkan region. The main goal of this presentation
is to improve awareness of great importance of HPV vaccination in target popullations.",
publisher = "Komora magistara farmacije Federacije Bosne i hercegovine, Farmaceutska komora Republike Srpske, Farmaceutsko društvo Republike Srpske",
journal = "5th Congress of Pharmacists of Bosnia and Herzegovina with international participation, Abstract book, „Competencies of pharmacists – from drug design to successful disease treatment“",
title = "About the HPV vaccine: how it works and who can get it",
pages = "35-36",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5226"
}

Filipić, B.. (2023). About the HPV vaccine: how it works and who can get it. in 5th Congress of Pharmacists of Bosnia and Herzegovina with international participation, Abstract book, „Competencies of pharmacists – from drug design to successful disease treatment“
Komora magistara farmacije Federacije Bosne i hercegovine., 35-36.
https://hdl.handle.net/21.15107/rcub_farfar_5226

Filipić B. About the HPV vaccine: how it works and who can get it. in 5th Congress of Pharmacists of Bosnia and Herzegovina with international participation, Abstract book, „Competencies of pharmacists – from drug design to successful disease treatment“. 2023;:35-36.
https://hdl.handle.net/21.15107/rcub_farfar_5226 .

Filipić, Brankica, "About the HPV vaccine: how it works and who can get it" in 5th Congress of Pharmacists of Bosnia and Herzegovina with international participation, Abstract book, „Competencies of pharmacists – from drug design to successful disease treatment“ (2023):35-36,
https://hdl.handle.net/21.15107/rcub_farfar_5226 .

TY  - GEN
AU  - Rajković, Dragana
AU  - Filipić, Brankica
AU  - Tadić, Ivana
AU  - Rakić, Milan
AU  - Trunić, Marina
AU  - Letić, Bojana
AU  - Stojiljković, Dragana
AU  - Urošević, Jasna
AU  - Šipetić, Tatjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5117
AB  - Uvod: Na osnovu podataka iz 2020, u Republici Srbiji je kod 1205 žena postavljena dijagnoza karcinoma grlića materice, dok su 643 žene preminule od ove bolesti. Karcinom grlića materice je peti po učestalosti karcinom kod žena u Srbiji i drugi po učestalosti karcinom kod žena između 15 i 44 godina starosti. ...
PB  - Farmaceutska komora Crne Gore
T2  - 4. kongres farmaceuta Crne Gore sa međunarodnim učešćem, 11-14. maj 2023 Budva, Bečići, Crna Gora
T1  - Jačanje uloge farmaceuta u promociji značaja HPV vakcinacije u Srbiji na nacionalnom i regionalnom nivou
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5117
ER  - 

@misc{
author = "Rajković, Dragana and Filipić, Brankica and Tadić, Ivana and Rakić, Milan and Trunić, Marina and Letić, Bojana and Stojiljković, Dragana and Urošević, Jasna and Šipetić, Tatjana",
year = "2023",
abstract = "Uvod: Na osnovu podataka iz 2020, u Republici Srbiji je kod 1205 žena postavljena dijagnoza karcinoma grlića materice, dok su 643 žene preminule od ove bolesti. Karcinom grlića materice je peti po učestalosti karcinom kod žena u Srbiji i drugi po učestalosti karcinom kod žena između 15 i 44 godina starosti. ...",
publisher = "Farmaceutska komora Crne Gore",
journal = "4. kongres farmaceuta Crne Gore sa međunarodnim učešćem, 11-14. maj 2023 Budva, Bečići, Crna Gora",
title = "Jačanje uloge farmaceuta u promociji značaja HPV vakcinacije u Srbiji na nacionalnom i regionalnom nivou",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5117"
}

Rajković, D., Filipić, B., Tadić, I., Rakić, M., Trunić, M., Letić, B., Stojiljković, D., Urošević, J.,& Šipetić, T.. (2023). Jačanje uloge farmaceuta u promociji značaja HPV vakcinacije u Srbiji na nacionalnom i regionalnom nivou. in 4. kongres farmaceuta Crne Gore sa međunarodnim učešćem, 11-14. maj 2023 Budva, Bečići, Crna Gora
Farmaceutska komora Crne Gore., 44-44.
https://hdl.handle.net/21.15107/rcub_farfar_5117

Rajković D, Filipić B, Tadić I, Rakić M, Trunić M, Letić B, Stojiljković D, Urošević J, Šipetić T. Jačanje uloge farmaceuta u promociji značaja HPV vakcinacije u Srbiji na nacionalnom i regionalnom nivou. in 4. kongres farmaceuta Crne Gore sa međunarodnim učešćem, 11-14. maj 2023 Budva, Bečići, Crna Gora. 2023;:44-44.
https://hdl.handle.net/21.15107/rcub_farfar_5117 .

Rajković, Dragana, Filipić, Brankica, Tadić, Ivana, Rakić, Milan, Trunić, Marina, Letić, Bojana, Stojiljković, Dragana, Urošević, Jasna, Šipetić, Tatjana, "Jačanje uloge farmaceuta u promociji značaja HPV vakcinacije u Srbiji na nacionalnom i regionalnom nivou" in 4. kongres farmaceuta Crne Gore sa međunarodnim učešćem, 11-14. maj 2023 Budva, Bečići, Crna Gora (2023):44-44,
https://hdl.handle.net/21.15107/rcub_farfar_5117 .

TY  - JOUR
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4471
AB  - Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia
VL  - 14
DO  - 10.3389/fmicb.2023.1094184
ER  - 

@article{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
abstract = "Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia",
volume = "14",
doi = "10.3389/fmicb.2023.1094184"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology
Frontiers Media S.A.., 14.
https://doi.org/10.3389/fmicb.2023.1094184

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology. 2023;14.
doi:10.3389/fmicb.2023.1094184 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia" in Frontiers in Microbiology, 14 (2023),
https://doi.org/10.3389/fmicb.2023.1094184 . .

TY  - CONF
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Bigović, Dubravka
AU  - Petrović, Predrag
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5120
AB  - Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
T1  - Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5120
ER  - 

@conference{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Bigović, Dubravka and Petrović, Predrag and Pljevljakušić, Dejan and Šavikin, Katarina and Filipić, Brankica",
year = "2022",
abstract = "Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts",
title = "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5120"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Bigović, D., Petrović, P., Pljevljakušić, D., Šavikin, K.,& Filipić, B.. (2022). Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
Belgrade : Institute of Technical Sciences of SASA., 14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120

Kuzmanović Nedeljković S, Ćujić Nikolić N, Bigović D, Petrović P, Pljevljakušić D, Šavikin K, Filipić B. Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts. 2022;:14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Bigović, Dubravka, Petrović, Predrag, Pljevljakušić, Dejan, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin" in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts (2022):14-14,
https://hdl.handle.net/21.15107/rcub_farfar_5120 .

TY  - CONF
AU  - Kuzmanović Nedeljković, Snežana
AU  - Stević, Tatjana
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5119
AB  - BACKGROUND
COVID-19 pandemic brought a burden to the health care system including a higher rate of patients admitted to the intensive care unit (ICU). Mechanical ventilation, needed in COVID-19 patients admitted to ICU, increases the risk of developing multidrug-resistant (MDR) Acinetobacter baumannii superinfection, which may cause major problems in antibiotic treatment.
OBJECTIVES
The aim of this research was to examine the susceptibility of A. baumannii isolates recovered from COVID-19 patients 
to antibiotics usually used as treatment options, and to Oregano essential oil (EO) as naturally derived compound.
METHODS 
The 54 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU, during period from December 
2020 to February 2021. Biofilm formation ability, as the factor that contributes to bacterial resistance, was examined. Minimum inhibitory concentrations (MICs) were determined for meropenem, imipenem, colistin, gentamicin, tobramycin and levofloxacin. In addition, all 54 isolates of A. baumanii were tested for susceptibility to Oregano EO.
RESULTS 
Results obtained in this study revealed a significant MDR profile of tested A. baumannii isolates. Even 74% (40/54) were strong biofilm producers. A. baumannii isolates were resistant to all tested antibiotics, except to colistin, leaving colistin as the only treatment option. On the other hand, MIC results obtained for Oregano EO were extremely promising: 0.279 mg/mL for all tested isolates. The obtained results emphasize a potentially new treatment approach to infections caused by MDR A. baumannii, using natural products such as EOs.
PB  - Federation of European Microbiological Societies
PB  - Serbian Society for Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
T1  - Oregano essential oil sensitivity of multiresistant Acinetobacter baumannii strains isolated from COVID-19 patients
SP  - 272
EP  - 272
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5119
ER  - 

@conference{
author = "Kuzmanović Nedeljković, Snežana and Stević, Tatjana and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2022",
abstract = "BACKGROUND
COVID-19 pandemic brought a burden to the health care system including a higher rate of patients admitted to the intensive care unit (ICU). Mechanical ventilation, needed in COVID-19 patients admitted to ICU, increases the risk of developing multidrug-resistant (MDR) Acinetobacter baumannii superinfection, which may cause major problems in antibiotic treatment.
OBJECTIVES
The aim of this research was to examine the susceptibility of A. baumannii isolates recovered from COVID-19 patients 
to antibiotics usually used as treatment options, and to Oregano essential oil (EO) as naturally derived compound.
METHODS 
The 54 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU, during period from December 
2020 to February 2021. Biofilm formation ability, as the factor that contributes to bacterial resistance, was examined. Minimum inhibitory concentrations (MICs) were determined for meropenem, imipenem, colistin, gentamicin, tobramycin and levofloxacin. In addition, all 54 isolates of A. baumanii were tested for susceptibility to Oregano EO.
RESULTS 
Results obtained in this study revealed a significant MDR profile of tested A. baumannii isolates. Even 74% (40/54) were strong biofilm producers. A. baumannii isolates were resistant to all tested antibiotics, except to colistin, leaving colistin as the only treatment option. On the other hand, MIC results obtained for Oregano EO were extremely promising: 0.279 mg/mL for all tested isolates. The obtained results emphasize a potentially new treatment approach to infections caused by MDR A. baumannii, using natural products such as EOs.",
publisher = "Federation of European Microbiological Societies, Serbian Society for Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book",
title = "Oregano essential oil sensitivity of multiresistant Acinetobacter baumannii strains isolated from COVID-19 patients",
pages = "272-272",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5119"
}

Kuzmanović Nedeljković, S., Stević, T., Bigović, D., Šavikin, K.,& Filipić, B.. (2022). Oregano essential oil sensitivity of multiresistant Acinetobacter baumannii strains isolated from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
Federation of European Microbiological Societies., 272-272.
https://hdl.handle.net/21.15107/rcub_farfar_5119

Kuzmanović Nedeljković S, Stević T, Bigović D, Šavikin K, Filipić B. Oregano essential oil sensitivity of multiresistant Acinetobacter baumannii strains isolated from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book. 2022;:272-272.
https://hdl.handle.net/21.15107/rcub_farfar_5119 .

Kuzmanović Nedeljković, Snežana, Stević, Tatjana, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Oregano essential oil sensitivity of multiresistant Acinetobacter baumannii strains isolated from COVID-19 patients" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book (2022):272-272,
https://hdl.handle.net/21.15107/rcub_farfar_5119 .

TY  - CONF
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5585
AB  - Koronavirusi pripadaju RNK virusima, a naziv potiče od latinske reči “corona” usled slič-
nosti sa vencem (koronom) sunčevih zraka. Sve do početka 21. veka, koronavirusi su povezi-
vani sa sezonskim prehladama i nisu bili prepoznati kao značajni humani patogeni.
Međutim, 2002. godine u Kini je identifikovan uzročnik teškog akutnog respiratornog sin-
droma – SARS (engl. Severe acute respiratory syndrome), pri čemu su zabeleženi smrtni isho-
di, a virus je označen kao SARS-CoV. Deset godina kasnije, 2012. godine, u Saudijskoj Arabiji
identifikovan je MERS-CoV (engl. Middle East respiratory syndrome Coronavirus), a krajem
2019. godine i novi koronavirus koji je kasnije označen kao SARS-CoV-2 usled sličnosti sa
SARS-CoV. Broj umrlih usled infekcije SARS-CoV-2 i razvoja bolesti COVID-19 (engl. coronavi-
rus disease 2019) procenjuje se do sada na oko 6 miliona ljudi globalno.
Usled brzog širenja SARS-CoV-2, Svetska Zdravstvena Organizacija objavila je 11. marta
2020. pandemiju, koja je još uvek na snazi. Pandemija je dovela do brzog odgovora na COVID-19
što je za posledicu imalo velika finansijska ulaganja u razvoj bolničkih kapaciteta, novih tera-
pijskih opcija, a posebno na razvoj vakcina protiv COVID-19. Međutim, paralelno sa razvojem i
primenom vakcina došlo je do mutacija u genomu SARS-CoV-2 i pojave novih varijanti virusa što
je posledično dovelo do manje efikasnosti vakcina i dostupnih terapijskih opcija.
U okviru izlaganja dat je uporedni pregled karakteristika virusa SARS-CoV, MERS-CoV i
SARS-CoV-2. Pažnja koju su privukli koronavirusi 21. veka i iskustva i znanja stečena njiho-
vom pojavom, omogući će bolji odgovor na potencijalno nove koronaviruse, kao i bolji odgo-
vor na epidemije ili pandemije uzrokovane drugim patogenima.
PB  - Udruženje farmaceuta Beograd
C3  - Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
T1  - Koronavirusi i 21. vek
SP  - 9
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5585
ER  - 

@conference{
author = "Filipić, Brankica",
year = "2022",
abstract = "Koronavirusi pripadaju RNK virusima, a naziv potiče od latinske reči “corona” usled slič-
nosti sa vencem (koronom) sunčevih zraka. Sve do početka 21. veka, koronavirusi su povezi-
vani sa sezonskim prehladama i nisu bili prepoznati kao značajni humani patogeni.
Međutim, 2002. godine u Kini je identifikovan uzročnik teškog akutnog respiratornog sin-
droma – SARS (engl. Severe acute respiratory syndrome), pri čemu su zabeleženi smrtni isho-
di, a virus je označen kao SARS-CoV. Deset godina kasnije, 2012. godine, u Saudijskoj Arabiji
identifikovan je MERS-CoV (engl. Middle East respiratory syndrome Coronavirus), a krajem
2019. godine i novi koronavirus koji je kasnije označen kao SARS-CoV-2 usled sličnosti sa
SARS-CoV. Broj umrlih usled infekcije SARS-CoV-2 i razvoja bolesti COVID-19 (engl. coronavi-
rus disease 2019) procenjuje se do sada na oko 6 miliona ljudi globalno.
Usled brzog širenja SARS-CoV-2, Svetska Zdravstvena Organizacija objavila je 11. marta
2020. pandemiju, koja je još uvek na snazi. Pandemija je dovela do brzog odgovora na COVID-19
što je za posledicu imalo velika finansijska ulaganja u razvoj bolničkih kapaciteta, novih tera-
pijskih opcija, a posebno na razvoj vakcina protiv COVID-19. Međutim, paralelno sa razvojem i
primenom vakcina došlo je do mutacija u genomu SARS-CoV-2 i pojave novih varijanti virusa što
je posledično dovelo do manje efikasnosti vakcina i dostupnih terapijskih opcija.
U okviru izlaganja dat je uporedni pregled karakteristika virusa SARS-CoV, MERS-CoV i
SARS-CoV-2. Pažnja koju su privukli koronavirusi 21. veka i iskustva i znanja stečena njiho-
vom pojavom, omogući će bolji odgovor na potencijalno nove koronaviruse, kao i bolji odgo-
vor na epidemije ili pandemije uzrokovane drugim patogenima.",
publisher = "Udruženje farmaceuta Beograd",
journal = "Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd",
title = "Koronavirusi i 21. vek",
pages = "9-9",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5585"
}

Filipić, B.. (2022). Koronavirusi i 21. vek. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
Udruženje farmaceuta Beograd., 9-9.
https://hdl.handle.net/21.15107/rcub_farfar_5585

Filipić B. Koronavirusi i 21. vek. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd. 2022;:9-9.
https://hdl.handle.net/21.15107/rcub_farfar_5585 .

Filipić, Brankica, "Koronavirusi i 21. vek" in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd (2022):9-9,
https://hdl.handle.net/21.15107/rcub_farfar_5585 .

TY  - CONF
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5227
AB  - Pandemija uzrokovana SARS-CoV-2 dovela je do sada do oko 6 miliona smrtnih
ishoda globalno. Usled hitnosti da se pandemija zaustavi, uloženi su veliki napori u razvoj
COVID-19 vakcina, s obzirom da vakcinacija predstavlja jedno od najefikasnijih oruđa za spre-
čavanje širenja infektivnih bolesti. Cilj ovog rada je da se napravi pregled COVID-19 vakcina
dostupnih u našoj zemlji.
PB  - Udruženje farmaceuta Beograd
C3  - Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
T1  - COVID-19 vakcine dostupne u Republici Srbiji
SP  - 23
EP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5227
ER  - 

@conference{
author = "Filipić, Brankica",
year = "2022",
abstract = "Pandemija uzrokovana SARS-CoV-2 dovela je do sada do oko 6 miliona smrtnih
ishoda globalno. Usled hitnosti da se pandemija zaustavi, uloženi su veliki napori u razvoj
COVID-19 vakcina, s obzirom da vakcinacija predstavlja jedno od najefikasnijih oruđa za spre-
čavanje širenja infektivnih bolesti. Cilj ovog rada je da se napravi pregled COVID-19 vakcina
dostupnih u našoj zemlji.",
publisher = "Udruženje farmaceuta Beograd",
journal = "Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd",
title = "COVID-19 vakcine dostupne u Republici Srbiji",
pages = "23-23",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5227"
}

Filipić, B.. (2022). COVID-19 vakcine dostupne u Republici Srbiji. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
Udruženje farmaceuta Beograd., 23-23.
https://hdl.handle.net/21.15107/rcub_farfar_5227

Filipić B. COVID-19 vakcine dostupne u Republici Srbiji. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd. 2022;:23-23.
https://hdl.handle.net/21.15107/rcub_farfar_5227 .

Filipić, Brankica, "COVID-19 vakcine dostupne u Republici Srbiji" in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd (2022):23-23,
https://hdl.handle.net/21.15107/rcub_farfar_5227 .

TY  - CONF
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena M.
AU  - Arsenović-Ranin, Nevena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5133
AB  - Colistin is used as a “last resort” drug in the treatment
of carbapenem-resistant Acinetobacter baumannii
infections. Though not the most convenient option due to
the serious side effects, this antibiotic can be very efficient
if administered properly (Garnacho-Montero and Timsit,
2019). However, for several years now, we are witnessing
a steady emergence of colistin-resistant (ColR) strains, with
the constant threat of a sudden burst worldwide (Nowak et
al., 2017). Having in mind that these strains are usually
non-susceptible to any of the existing treatments, i.e.
pandrug-resistant (PDR), the development of new
therapeutic strategies is desperately needed.
In the present study, we showed that colistin
susceptibility of ColR A. baumannii strains can be fully
restored in the presence of very low amounts of selenium
nanoparticles (SeNPs). To demonstrate potent synergistic
interactions between colistin and SeNPs, we performed
checkerboard and time-kill analyses.
PB  - Macedonian Pharmaceutical Association
C3  - Macedonian Pharmaceutical Bulletin
T1  - Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin
VL  - 68
IS  - Suppl. 1
SP  - 295
EP  - 296
DO  - 10.33320/maced.pharm.bull.2022.68.03.142
ER  - 

@conference{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena M. and Arsenović-Ranin, Nevena and Milenković, Marina",
year = "2022",
abstract = "Colistin is used as a “last resort” drug in the treatment
of carbapenem-resistant Acinetobacter baumannii
infections. Though not the most convenient option due to
the serious side effects, this antibiotic can be very efficient
if administered properly (Garnacho-Montero and Timsit,
2019). However, for several years now, we are witnessing
a steady emergence of colistin-resistant (ColR) strains, with
the constant threat of a sudden burst worldwide (Nowak et
al., 2017). Having in mind that these strains are usually
non-susceptible to any of the existing treatments, i.e.
pandrug-resistant (PDR), the development of new
therapeutic strategies is desperately needed.
In the present study, we showed that colistin
susceptibility of ColR A. baumannii strains can be fully
restored in the presence of very low amounts of selenium
nanoparticles (SeNPs). To demonstrate potent synergistic
interactions between colistin and SeNPs, we performed
checkerboard and time-kill analyses.",
publisher = "Macedonian Pharmaceutical Association",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin",
volume = "68",
number = "Suppl. 1",
pages = "295-296",
doi = "10.33320/maced.pharm.bull.2022.68.03.142"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M. M., Arsenović-Ranin, N.,& Milenković, M.. (2022). Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(Suppl. 1), 295-296.
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović MM, Arsenović-Ranin N, Milenković M. Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin. 2022;68(Suppl. 1):295-296.
doi:10.33320/maced.pharm.bull.2022.68.03.142 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena M., Arsenović-Ranin, Nevena, Milenković, Marina, "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin" in Macedonian Pharmaceutical Bulletin, 68, no. Suppl. 1 (2022):295-296,
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Malešević, Milka
AU  - Vasiljević, Zorica
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4370
AB  - Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.
PB  - Springer Science and Business Media B.V.
T2  - Folia Microbiologica
T1  - Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene
VL  - n/d
DO  - 10.1007/s12223-022-01026-8
ER  - 

@article{
author = "Filipić, Brankica and Malešević, Milka and Vasiljević, Zorica and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "Folia Microbiologica",
title = "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene",
volume = "n/d",
doi = "10.1007/s12223-022-01026-8"
}

Filipić, B., Malešević, M., Vasiljević, Z., Novović, K., Kojić, M.,& Jovčić, B.. (2022). Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica
Springer Science and Business Media B.V.., n/d.
https://doi.org/10.1007/s12223-022-01026-8

Filipić B, Malešević M, Vasiljević Z, Novović K, Kojić M, Jovčić B. Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica. 2022;n/d.
doi:10.1007/s12223-022-01026-8 .

Filipić, Brankica, Malešević, Milka, Vasiljević, Zorica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene" in Folia Microbiologica, n/d (2022),
https://doi.org/10.1007/s12223-022-01026-8 . .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4177
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
VL  - 133
IS  - 3
SP  - 1197
SP  - 1197
EP  - 1206
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
volume = "133",
number = "3",
pages = "1197-1197-1206",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - CONF
AU  - Kabić, Jovana
AU  - Luković, Bojana
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Kekić, Dušan
AU  - Jovičević, Miloš
AU  - Ranin, Lazar
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Gajić, Ina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5139
PB  - ESCMID
C3  - 32nd European Congress of Clinical Microbiology and Infectious Diseases, 23-26 April 2022 Lisabon - Final Programme
T1  - A core genome multilocus sequence typing of Acinetobacter baumannii isolated from pre-COVID-19 and COVID-19 period
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5139
ER  - 

@conference{
author = "Kabić, Jovana and Luković, Bojana and Jovčić, Branko and Filipić, Brankica and Kekić, Dušan and Jovičević, Miloš and Ranin, Lazar and Opavski, Nataša and Dimkić, Ivica and Gajić, Ina",
year = "2022",
publisher = "ESCMID",
journal = "32nd European Congress of Clinical Microbiology and Infectious Diseases, 23-26 April 2022 Lisabon - Final Programme",
title = "A core genome multilocus sequence typing of Acinetobacter baumannii isolated from pre-COVID-19 and COVID-19 period",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5139"
}

Kabić, J., Luković, B., Jovčić, B., Filipić, B., Kekić, D., Jovičević, M., Ranin, L., Opavski, N., Dimkić, I.,& Gajić, I.. (2022). A core genome multilocus sequence typing of Acinetobacter baumannii isolated from pre-COVID-19 and COVID-19 period. in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 23-26 April 2022 Lisabon - Final Programme
ESCMID..
https://hdl.handle.net/21.15107/rcub_farfar_5139

Kabić J, Luković B, Jovčić B, Filipić B, Kekić D, Jovičević M, Ranin L, Opavski N, Dimkić I, Gajić I. A core genome multilocus sequence typing of Acinetobacter baumannii isolated from pre-COVID-19 and COVID-19 period. in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 23-26 April 2022 Lisabon - Final Programme. 2022;.
https://hdl.handle.net/21.15107/rcub_farfar_5139 .

Kabić, Jovana, Luković, Bojana, Jovčić, Branko, Filipić, Brankica, Kekić, Dušan, Jovičević, Miloš, Ranin, Lazar, Opavski, Nataša, Dimkić, Ivica, Gajić, Ina, "A core genome multilocus sequence typing of Acinetobacter baumannii isolated from pre-COVID-19 and COVID-19 period" in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 23-26 April 2022 Lisabon - Final Programme (2022),
https://hdl.handle.net/21.15107/rcub_farfar_5139 .

TY  - CONF
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Jovčić, Branko
AU  - Kojić, Milan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5118
AB  - BACKGROUND
From the start of COVID-19 pandemic, many patients suffering severe clinical presentation of COVID-19 and 
acute respiratory failure have been administered to intensive care units (ICUs). Need for noninvasive or invasive 
mechanical ventilation represents a high-risk factor for these patients to develop Acinetobacter baumannii 
superinfection.
OBJECTIVES
The main goal of this study was to examine virulence potential of A. baumannii isolates recovered from 
COVID-19 patients admitted to ICU as well as to compare differences in virulence among isolates originated 
from male and female patients or from different sample types.
METHODS
64 A. baumannii isolates were recovered from COVID-19 patients from December 2020 to February 2021. 
The isolates were originated from different sample types: blood, tip of the central venous catheter, tracheal 
aspirate, tip of the aspirator and sputum. Genetic relatedness of ApaI digested A. baumannii isolates, 
was determined by pulsed-field gel electrophoresis (PFGE). Affinity to mucin binding was tested using in vitro 
model and isolates were examined for two types of motilities: swarming and twitching.
PB  - Federation of European Microbiological Societies
PB  - Serbian Society for Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
T1  - Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients
SP  - 96
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5118
ER  - 

@conference{
author = "Filipić, Brankica and Novović, Katarina and Kuzmanović Nedeljković, Snežana and Jovčić, Branko and Kojić, Milan",
year = "2022",
abstract = "BACKGROUND
From the start of COVID-19 pandemic, many patients suffering severe clinical presentation of COVID-19 and 
acute respiratory failure have been administered to intensive care units (ICUs). Need for noninvasive or invasive 
mechanical ventilation represents a high-risk factor for these patients to develop Acinetobacter baumannii 
superinfection.
OBJECTIVES
The main goal of this study was to examine virulence potential of A. baumannii isolates recovered from 
COVID-19 patients admitted to ICU as well as to compare differences in virulence among isolates originated 
from male and female patients or from different sample types.
METHODS
64 A. baumannii isolates were recovered from COVID-19 patients from December 2020 to February 2021. 
The isolates were originated from different sample types: blood, tip of the central venous catheter, tracheal 
aspirate, tip of the aspirator and sputum. Genetic relatedness of ApaI digested A. baumannii isolates, 
was determined by pulsed-field gel electrophoresis (PFGE). Affinity to mucin binding was tested using in vitro 
model and isolates were examined for two types of motilities: swarming and twitching.",
publisher = "Federation of European Microbiological Societies, Serbian Society for Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book",
title = "Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5118"
}

Filipić, B., Novović, K., Kuzmanović Nedeljković, S., Jovčić, B.,& Kojić, M.. (2022). Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book
Federation of European Microbiological Societies., 96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5118

Filipić B, Novović K, Kuzmanović Nedeljković S, Jovčić B, Kojić M. Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book. 2022;:96-96.
https://hdl.handle.net/21.15107/rcub_farfar_5118 .

Filipić, Brankica, Novović, Katarina, Kuzmanović Nedeljković, Snežana, Jovčić, Branko, Kojić, Milan, "Assesing virulence potential of Acinetobacter baumannii isolates recovered from COVID-19 patients" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 30 June - 2 July 2022, Serbia - electronic abstract book (2022):96-96,
https://hdl.handle.net/21.15107/rcub_farfar_5118 .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Miljković, Marija
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Studholme, David
AU  - Kojić, Milan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3729
AB  - Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis bv. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid pS74 is EPS production, while plasmid pS127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid pS127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.
PB  - Elsevier B.V.
T2  - International Journal of Food Microbiology
T1  - The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties
VL  - 337
DO  - 10.1016/j.ijfoodmicro.2020.108935
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Miljković, Marija and Jovčić, Branko and Filipić, Brankica and Studholme, David and Kojić, Milan",
year = "2021",
abstract = "Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis bv. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid pS74 is EPS production, while plasmid pS127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid pS127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Food Microbiology",
title = "The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties",
volume = "337",
doi = "10.1016/j.ijfoodmicro.2020.108935"
}

Malešević, M., Stanisavljević, N., Miljković, M., Jovčić, B., Filipić, B., Studholme, D.,& Kojić, M.. (2021). The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology
Elsevier B.V.., 337.
https://doi.org/10.1016/j.ijfoodmicro.2020.108935

Malešević M, Stanisavljević N, Miljković M, Jovčić B, Filipić B, Studholme D, Kojić M. The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology. 2021;337.
doi:10.1016/j.ijfoodmicro.2020.108935 .

Malešević, Milka, Stanisavljević, Nemanja, Miljković, Marija, Jovčić, Branko, Filipić, Brankica, Studholme, David, Kojić, Milan, "The large plasmidome of Lactococcus lactis subsp. lactis bv. diacetylactis S50 confers its biotechnological properties" in International Journal of Food Microbiology, 337 (2021),
https://doi.org/10.1016/j.ijfoodmicro.2020.108935 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Obradović, Mina
AU  - O’Connor, Paula M.
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3932
AB  - Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.
PB  - Springer Nature
T2  - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
T1  - C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae
VL  - 114
SP  - 1595
EP  - 1607
DO  - 10.1007/s10482-021-01626-3
ER  - 

@article{
author = "Mirković, Nemanja and Obradović, Mina and O’Connor, Paula M. and Filipić, Brankica and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2021",
abstract = "Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.",
publisher = "Springer Nature",
journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",
title = "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae",
volume = "114",
pages = "1595-1607",
doi = "10.1007/s10482-021-01626-3"
}

Mirković, N., Obradović, M., O’Connor, P. M., Filipić, B., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2021). C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Springer Nature., 114, 1595-1607.
https://doi.org/10.1007/s10482-021-01626-3

Mirković N, Obradović M, O’Connor PM, Filipić B, Jovčić B, Cotter PD, Kojić M. C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2021;114:1595-1607.
doi:10.1007/s10482-021-01626-3 .

Mirković, Nemanja, Obradović, Mina, O’Connor, Paula M., Filipić, Brankica, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae" in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 114 (2021):1595-1607,
https://doi.org/10.1007/s10482-021-01626-3 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Studholme, David
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3631
AB  - Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.
PB  - NLM (Medline)
T2  - Journal of water and health
T1  - Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments
VL  - 18
IS  - 3
SP  - 383
EP  - 397
DO  - 10.2166/wh.2020.227
ER  - 

@article{
author = "Filipić, Brankica and Novović, Katarina and Studholme, David and Malešević, Milka and Mirković, Nemanja and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.",
publisher = "NLM (Medline)",
journal = "Journal of water and health",
title = "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments",
volume = "18",
number = "3",
pages = "383-397",
doi = "10.2166/wh.2020.227"
}

Filipić, B., Novović, K., Studholme, D., Malešević, M., Mirković, N., Kojić, M.,& Jovčić, B.. (2020). Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health
NLM (Medline)., 18(3), 383-397.
https://doi.org/10.2166/wh.2020.227

Filipić B, Novović K, Studholme D, Malešević M, Mirković N, Kojić M, Jovčić B. Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health. 2020;18(3):383-397.
doi:10.2166/wh.2020.227 .

Filipić, Brankica, Novović, Katarina, Studholme, David, Malešević, Milka, Mirković, Nemanja, Kojić, Milan, Jovčić, Branko, "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments" in Journal of water and health, 18, no. 3 (2020):383-397,
https://doi.org/10.2166/wh.2020.227 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Velhner, Maja
AU  - Todorović, Dalibor
AU  - Matović, Kazimir
AU  - Rašić, Zoran
AU  - Nikolić, Sonja
AU  - Kiškarolj, Ferenc
AU  - Kojić, Milan
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3760
AB  - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
PB  - MDPI AG
T2  - Antibiotics
T1  - Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia
VL  - 9
IS  - 12
SP  - 1
EP  - 13
DO  - 10.3390/antibiotics9120886
ER  - 

@article{
author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rašić, Zoran and Nikolić, Sonja and Kiškarolj, Ferenc and Kojić, Milan",
year = "2020",
abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.",
publisher = "MDPI AG",
journal = "Antibiotics",
title = "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia",
volume = "9",
number = "12",
pages = "1-13",
doi = "10.3390/antibiotics9120886"
}

Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rašić, Z., Nikolić, S., Kiškarolj, F.,& Kojić, M.. (2020). Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics
MDPI AG., 9(12), 1-13.
https://doi.org/10.3390/antibiotics9120886

Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rašić Z, Nikolić S, Kiškarolj F, Kojić M. Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics. 2020;9(12):1-13.
doi:10.3390/antibiotics9120886 .

Jovčić, Branko, Novović, Katarina, Filipić, Brankica, Velhner, Maja, Todorović, Dalibor, Matović, Kazimir, Rašić, Zoran, Nikolić, Sonja, Kiškarolj, Ferenc, Kojić, Milan, "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia" in Antibiotics, 9, no. 12 (2020):1-13,
https://doi.org/10.3390/antibiotics9120886 . .