TY  - JOUR
AU  - Kovačević, Pedja
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Barišić, Vedrana
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
AU  - Rizwan, Zeeshan
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5631
AB  - Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Naunyn-Schmiedeberg's Archives of Pharmacology
T1  - Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study
DO  - 10.1007/s00210-024-03136-1
ER  - 

@article{
author = "Kovačević, Pedja and Milaković, Dragana and Kovačević, Tijana and Barišić, Vedrana and Dragić, Saša and Zlojutro, Biljana and Miljković, Branislava and Vučićević, Katarina and Rizwan, Zeeshan",
abstract = "Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Naunyn-Schmiedeberg's Archives of Pharmacology",
title = "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study",
doi = "10.1007/s00210-024-03136-1"
}

Kovačević, P., Milaković, D., Kovačević, T., Barišić, V., Dragić, S., Zlojutro, B., Miljković, B., Vučićević, K.,& Rizwan, Z..Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00210-024-03136-1

Kovačević P, Milaković D, Kovačević T, Barišić V, Dragić S, Zlojutro B, Miljković B, Vučićević K, Rizwan Z. Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology..
doi:10.1007/s00210-024-03136-1 .

Kovačević, Pedja, Milaković, Dragana, Kovačević, Tijana, Barišić, Vedrana, Dragić, Saša, Zlojutro, Biljana, Miljković, Branislava, Vučićević, Katarina, Rizwan, Zeeshan, "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study" in Naunyn-Schmiedeberg's Archives of Pharmacology,
https://doi.org/10.1007/s00210-024-03136-1 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I..Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines.12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4,
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Vasilić, Đorđe
AU  - Rašević, Marija
AU  - Zečević, Mira
AU  - Čarapić, Marija
AU  - Malenović, Anđelija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5633
AB  - In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis.
AB  - Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis
T1  - Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova
VL  - 74
IS  - 2
SP  - 160
EP  - 177
ER  - 

@article{
author = "Vasilić, Đorđe and Rašević, Marija and Zečević, Mira and Čarapić, Marija and Malenović, Anđelija",
year = "2024",
abstract = "In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis., Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis, Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova",
volume = "74",
number = "2",
pages = "160-177"
}

Vasilić, Đ., Rašević, M., Zečević, M., Čarapić, M.,& Malenović, A.. (2024). Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 160-177.

Vasilić Đ, Rašević M, Zečević M, Čarapić M, Malenović A. Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju. 2024;74(2):160-177..

Vasilić, Đorđe, Rašević, Marija, Zečević, Mira, Čarapić, Marija, Malenović, Anđelija, "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis" in Arhiv za farmaciju, 74, no. 2 (2024):160-177.

TY  - JOUR
AU  - Bećirović, Sabina
AU  - Vuković, Bojana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5632
AB  - The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.
PB  - Taylor and Francis Ltd.
T2  - Biotechnology and Biotechnological Equipment
T1  - Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina
VL  - 38
IS  - 1
DO  - 10.1080/13102818.2024.2349593
ER  - 

@article{
author = "Bećirović, Sabina and Vuković, Bojana and Marinković, Valentina",
year = "2024",
abstract = "The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.",
publisher = "Taylor and Francis Ltd.",
journal = "Biotechnology and Biotechnological Equipment",
title = "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina",
volume = "38",
number = "1",
doi = "10.1080/13102818.2024.2349593"
}

Bećirović, S., Vuković, B.,& Marinković, V.. (2024). Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment
Taylor and Francis Ltd.., 38(1).
https://doi.org/10.1080/13102818.2024.2349593

Bećirović S, Vuković B, Marinković V. Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment. 2024;38(1).
doi:10.1080/13102818.2024.2349593 .

Bećirović, Sabina, Vuković, Bojana, Marinković, Valentina, "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina" in Biotechnology and Biotechnological Equipment, 38, no. 1 (2024),
https://doi.org/10.1080/13102818.2024.2349593 . .

TY  - JOUR
AU  - Adamov, Ivana
AU  - Stanojević, Gordana
AU  - Pavlović, Stefan M.
AU  - Medarević, Đorđe
AU  - Ivković, Branka
AU  - Kočović, David
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5630
AB  - Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets
VL  - 657
DO  - 10.1016/j.ijpharm.2024.124161
ER  - 

@article{
author = "Adamov, Ivana and Stanojević, Gordana and Pavlović, Stefan M. and Medarević, Đorđe and Ivković, Branka and Kočović, David and Ibrić, Svetlana",
year = "2024",
abstract = "Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets",
volume = "657",
doi = "10.1016/j.ijpharm.2024.124161"
}

Adamov, I., Stanojević, G., Pavlović, S. M., Medarević, Đ., Ivković, B., Kočović, D.,& Ibrić, S.. (2024). Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics
Elsevier B.V.., 657.
https://doi.org/10.1016/j.ijpharm.2024.124161

Adamov I, Stanojević G, Pavlović SM, Medarević Đ, Ivković B, Kočović D, Ibrić S. Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics. 2024;657.
doi:10.1016/j.ijpharm.2024.124161 .

Adamov, Ivana, Stanojević, Gordana, Pavlović, Stefan M., Medarević, Đorđe, Ivković, Branka, Kočović, David, Ibrić, Svetlana, "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets" in International Journal of Pharmaceutics, 657 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124161 . .

TY  - CHAP
AU  - Medarević, Đorđe
AU  - Krstić, Mirjana
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5607
AB  - With the progress in medicine during the last several decades, it became clear
that the course of the disease and response to applied therapy differ to a great
extent between patients due to differences in genetic, anatomical, and physiological
characteristics of the patients. This brought into question the current
concept of therapy, so-called one size fits all, where similar drug doses have
been prescribed to a huge number of patients, with neglect of interindividual
differences between patients.
PB  - Elsevier Inc.
T2  - From Current to Future Trends in Pharmaceutical Technology
T1  - Fundamentals of 3D printing of pharmaceuticals
SP  - 1
EP  - 65
DO  - 10.1016/B978-0-323-91111-5.00001-9
ER  - 

@inbook{
author = "Medarević, Đorđe and Krstić, Mirjana and Ibrić, Svetlana",
year = "2024",
abstract = "With the progress in medicine during the last several decades, it became clear
that the course of the disease and response to applied therapy differ to a great
extent between patients due to differences in genetic, anatomical, and physiological
characteristics of the patients. This brought into question the current
concept of therapy, so-called one size fits all, where similar drug doses have
been prescribed to a huge number of patients, with neglect of interindividual
differences between patients.",
publisher = "Elsevier Inc.",
journal = "From Current to Future Trends in Pharmaceutical Technology",
booktitle = "Fundamentals of 3D printing of pharmaceuticals",
pages = "1-65",
doi = "10.1016/B978-0-323-91111-5.00001-9"
}

Medarević, Đ., Krstić, M.,& Ibrić, S.. (2024). Fundamentals of 3D printing of pharmaceuticals. in From Current to Future Trends in Pharmaceutical Technology
Elsevier Inc.., 1-65.
https://doi.org/10.1016/B978-0-323-91111-5.00001-9

Medarević Đ, Krstić M, Ibrić S. Fundamentals of 3D printing of pharmaceuticals. in From Current to Future Trends in Pharmaceutical Technology. 2024;:1-65.
doi:10.1016/B978-0-323-91111-5.00001-9 .

Medarević, Đorđe, Krstić, Mirjana, Ibrić, Svetlana, "Fundamentals of 3D printing of pharmaceuticals" in From Current to Future Trends in Pharmaceutical Technology (2024):1-65,
https://doi.org/10.1016/B978-0-323-91111-5.00001-9 . .

TY  - JOUR
AU  - Tasić, Gordana
AU  - Mitrović, Nikola
AU  - Simić, Milena
AU  - Koravović, Mladen
AU  - Jovanović, Predrag
AU  - Petković, Miloš
AU  - Jovanović, Miloš
AU  - Ivković, Branka
AU  - Savić, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5556
AB  - Hydantoin derivatives are versatile structural motifs found in natural products
and various compounds with different biological or other properties. Due to
their importance in both organic and medicinal chemistry, a number of synthetic procedures have been developed. In this article, a novel methodology
utilizing N-Boc protected amino acid amides for their preparation has been
described. The cyclisation process was accomplished using solid supported
PPh3 and CBr 4 as reagents affording substituted hydantoins in moderate to
good yields (40%–77%).
PB  - Wiley Periodicals LLC.
T2  - Journal Heterocyclic Chemistry
T1  - Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent
DO  - 10.1002/jhet.4802
ER  - 

@article{
author = "Tasić, Gordana and Mitrović, Nikola and Simić, Milena and Koravović, Mladen and Jovanović, Predrag and Petković, Miloš and Jovanović, Miloš and Ivković, Branka and Savić, Vladimir",
year = "2024",
abstract = "Hydantoin derivatives are versatile structural motifs found in natural products
and various compounds with different biological or other properties. Due to
their importance in both organic and medicinal chemistry, a number of synthetic procedures have been developed. In this article, a novel methodology
utilizing N-Boc protected amino acid amides for their preparation has been
described. The cyclisation process was accomplished using solid supported
PPh3 and CBr 4 as reagents affording substituted hydantoins in moderate to
good yields (40%–77%).",
publisher = "Wiley Periodicals LLC.",
journal = "Journal Heterocyclic Chemistry",
title = "Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent",
doi = "10.1002/jhet.4802"
}

Tasić, G., Mitrović, N., Simić, M., Koravović, M., Jovanović, P., Petković, M., Jovanović, M., Ivković, B.,& Savić, V.. (2024). Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent. in Journal Heterocyclic Chemistry
Wiley Periodicals LLC...
https://doi.org/10.1002/jhet.4802

Tasić G, Mitrović N, Simić M, Koravović M, Jovanović P, Petković M, Jovanović M, Ivković B, Savić V. Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent. in Journal Heterocyclic Chemistry. 2024;.
doi:10.1002/jhet.4802 .

Tasić, Gordana, Mitrović, Nikola, Simić, Milena, Koravović, Mladen, Jovanović, Predrag, Petković, Miloš, Jovanović, Miloš, Ivković, Branka, Savić, Vladimir, "Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent" in Journal Heterocyclic Chemistry (2024),
https://doi.org/10.1002/jhet.4802 . .

TY  - JOUR
AU  - Oljačić, Slavica
AU  - Križman, Mitja
AU  - Popović-Nikolić, Marija
AU  - Vovk, Irena
AU  - Nikolić, Katarina
AU  - Agbaba, Danica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5629
AB  - Background and Purpose: The ligands of the imidazoline and α-adrenergic receptors are mainly imidazoline and guanidine derivatives, known as centrally-acting antihypertensives and compounds with potential use in various neurological disorders. The extent of their ionisation has a major influence on their behaviour in the different analytical systems. The main objective of this work was to compare the mechanism of chromatographic retention and electrophoretic mobility under acidic, neutral and basic conditions. Experimental Approach: Multiple Linear Regression and Partial Least Squares Regression were applied for the QSRR (quantitative structure-retention relationship) and QSMR (quantitative structure-mobility relationship) modelling and to select the most important molecular parameters describing the chromatographic and electrophoretic behaviour of the investigated compounds. Key Results: The most important molecular descriptors, such as the chemical composition of the compounds, lipophilicity, polarizability and molecular branching, in the selected QSRR models showed that an important insight into the retention behaviour can be derived from the 0D-, 1D- and 2D-descriptors. The electrophoretic mobility could be explained by 2D- and 3D-descriptors, which provide information on the molecular mass, size and complexity, as well as on the influence of charge transfer and electronic properties on the migration behaviour. Conclusion: All created QSRR/QSMR models met the stringent validation criteria and showed high potential in describing the chromatographic and electrophoretic behaviour of investigated compounds.
PB  - International Association of Physical Chemists
T2  - ADMET and DMPK
T1  - Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions
DO  - 10.5599/admet.2278
ER  - 

@article{
author = "Oljačić, Slavica and Križman, Mitja and Popović-Nikolić, Marija and Vovk, Irena and Nikolić, Katarina and Agbaba, Danica",
year = "2024",
abstract = "Background and Purpose: The ligands of the imidazoline and α-adrenergic receptors are mainly imidazoline and guanidine derivatives, known as centrally-acting antihypertensives and compounds with potential use in various neurological disorders. The extent of their ionisation has a major influence on their behaviour in the different analytical systems. The main objective of this work was to compare the mechanism of chromatographic retention and electrophoretic mobility under acidic, neutral and basic conditions. Experimental Approach: Multiple Linear Regression and Partial Least Squares Regression were applied for the QSRR (quantitative structure-retention relationship) and QSMR (quantitative structure-mobility relationship) modelling and to select the most important molecular parameters describing the chromatographic and electrophoretic behaviour of the investigated compounds. Key Results: The most important molecular descriptors, such as the chemical composition of the compounds, lipophilicity, polarizability and molecular branching, in the selected QSRR models showed that an important insight into the retention behaviour can be derived from the 0D-, 1D- and 2D-descriptors. The electrophoretic mobility could be explained by 2D- and 3D-descriptors, which provide information on the molecular mass, size and complexity, as well as on the influence of charge transfer and electronic properties on the migration behaviour. Conclusion: All created QSRR/QSMR models met the stringent validation criteria and showed high potential in describing the chromatographic and electrophoretic behaviour of investigated compounds.",
publisher = "International Association of Physical Chemists",
journal = "ADMET and DMPK",
title = "Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions",
doi = "10.5599/admet.2278"
}

Oljačić, S., Križman, M., Popović-Nikolić, M., Vovk, I., Nikolić, K.,& Agbaba, D.. (2024). Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions. in ADMET and DMPK
International Association of Physical Chemists..
https://doi.org/10.5599/admet.2278

Oljačić S, Križman M, Popović-Nikolić M, Vovk I, Nikolić K, Agbaba D. Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions. in ADMET and DMPK. 2024;.
doi:10.5599/admet.2278 .

Oljačić, Slavica, Križman, Mitja, Popović-Nikolić, Marija, Vovk, Irena, Nikolić, Katarina, Agbaba, Danica, "Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions" in ADMET and DMPK (2024),
https://doi.org/10.5599/admet.2278 . .

TY  - JOUR
AU  - Popović-Nikolić, Marija
AU  - Čakar, Mira
AU  - Todorović, Nina
AU  - Nikolić, Katarina
AU  - Popović, Gordana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5589
AB  - The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.
PB  - Springer Nature
T2  - Chemical Papers
T1  - Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride
DO  - 10.1007/s11696-024-03399-1
ER  - 

@article{
author = "Popović-Nikolić, Marija and Čakar, Mira and Todorović, Nina and Nikolić, Katarina and Popović, Gordana",
year = "2024",
abstract = "The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.",
publisher = "Springer Nature",
journal = "Chemical Papers",
title = "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride",
doi = "10.1007/s11696-024-03399-1"
}

Popović-Nikolić, M., Čakar, M., Todorović, N., Nikolić, K.,& Popović, G.. (2024). Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers
Springer Nature..
https://doi.org/10.1007/s11696-024-03399-1

Popović-Nikolić M, Čakar M, Todorović N, Nikolić K, Popović G. Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers. 2024;.
doi:10.1007/s11696-024-03399-1 .

Popović-Nikolić, Marija, Čakar, Mira, Todorović, Nina, Nikolić, Katarina, Popović, Gordana, "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride" in Chemical Papers (2024),
https://doi.org/10.1007/s11696-024-03399-1 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Ušjak, Dušan
AU  - Hrast Rambaher, Martina
AU  - Oljačić, Slavica
AU  - Milenković, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5574
AB  - Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.
PB  - Frontiers Media SA
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Evaluation of novel compounds as anti-bacterial or anti-virulence agents
VL  - 14
DO  - 10.3389/fcimb.2024.1370062
ER  - 

@article{
author = "Filipić, Brankica and Ušjak, Dušan and Hrast Rambaher, Martina and Oljačić, Slavica and Milenković, Marina",
year = "2024",
abstract = "Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Evaluation of novel compounds as anti-bacterial or anti-virulence agents",
volume = "14",
doi = "10.3389/fcimb.2024.1370062"
}

Filipić, B., Ušjak, D., Hrast Rambaher, M., Oljačić, S.,& Milenković, M.. (2024). Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology
Frontiers Media SA., 14.
https://doi.org/10.3389/fcimb.2024.1370062

Filipić B, Ušjak D, Hrast Rambaher M, Oljačić S, Milenković M. Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology. 2024;14.
doi:10.3389/fcimb.2024.1370062 .

Filipić, Brankica, Ušjak, Dušan, Hrast Rambaher, Martina, Oljačić, Slavica, Milenković, Marina, "Evaluation of novel compounds as anti-bacterial or anti-virulence agents" in Frontiers in Cellular and Infection Microbiology, 14 (2024),
https://doi.org/10.3389/fcimb.2024.1370062 . .

TY  - JOUR
AU  - Živančević, Katarina
AU  - Živanović, Jovana
AU  - Baralić, Katarina
AU  - Božić, Dragica
AU  - Marić, Đurđica
AU  - Vukelić, Dragana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5624
AB  - The effect of the lead (Pb), cadmium (Cd), mercury (Hg) and arsenic (As) mixture (MIX) on hematotoxicity development was investigated trough combined approach. In vivo subacute study (28 days) was performed on rats (5 per group): a control group and five groups orally exposed to increasing metal(loid) mixture doses, MIX 1- MIX 5 (mg/kg bw./day) (Pb: 0.003, 0.01, 0.1, 0.3, 1; Cd: 0.01, 0.03, 0.3, 0.9, 3; Hg: 0.0002, 0.0006, 0.006, 0.018, 0.06; As: 0.002, 0.006, 0.06, 0.18, 0.6). Blood was taken for analysis of hematological parameters and serum iron (Fe) analysis. MIX treatment increased thrombocyte/platelet count and MCHC and decreased Hb, HCT, MCV and MCH values compared to control, indicating the development of anemia and thrombocytosis. BMDIs with the narrowest width were identified for MCH [pg] (6.030E-03 - 1.287E-01 mg Pb/kg bw./day; 2.010E-02 - 4.290E-01 mg Cd/kg bw./day; 4.020E-04 - 8.580E-03 mg Hg/kg bw./day; 4.020E-03 - 8.580E-02 mg As/kg bw./day). In silico analysis showed target genes connected with MIX and the development of: anemia - ACHE, GSR, PARP1, TNF; thrombocytosis – JAK2, CALR, MPL, THPO; hematological diseases - FAS and ALAD. The main extracted pathways for anemia were related to apoptosis and oxidative stress; for thrombocytosis were signaling pathways of Jak-STAT and TPO. Changes in miRNAs and transcription factors enabled the mode of action (MoA) development based on the obtained results, contributing to mechanistic understanding and hematological risk related to MIX exposure.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Integrative investigation of hematotoxic effects induced by low doses of
lead, cadmium, mercury and arsenic mixture: In vivo and in silico approach
VL  - 930
DO  - 10.1016/j.scitotenv.2024.172608
DO  - 2-s2.0-85191489918
ER  - 

@article{
author = "Živančević, Katarina and Živanović, Jovana and Baralić, Katarina and Božić, Dragica and Marić, Đurđica and Vukelić, Dragana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "The effect of the lead (Pb), cadmium (Cd), mercury (Hg) and arsenic (As) mixture (MIX) on hematotoxicity development was investigated trough combined approach. In vivo subacute study (28 days) was performed on rats (5 per group): a control group and five groups orally exposed to increasing metal(loid) mixture doses, MIX 1- MIX 5 (mg/kg bw./day) (Pb: 0.003, 0.01, 0.1, 0.3, 1; Cd: 0.01, 0.03, 0.3, 0.9, 3; Hg: 0.0002, 0.0006, 0.006, 0.018, 0.06; As: 0.002, 0.006, 0.06, 0.18, 0.6). Blood was taken for analysis of hematological parameters and serum iron (Fe) analysis. MIX treatment increased thrombocyte/platelet count and MCHC and decreased Hb, HCT, MCV and MCH values compared to control, indicating the development of anemia and thrombocytosis. BMDIs with the narrowest width were identified for MCH [pg] (6.030E-03 - 1.287E-01 mg Pb/kg bw./day; 2.010E-02 - 4.290E-01 mg Cd/kg bw./day; 4.020E-04 - 8.580E-03 mg Hg/kg bw./day; 4.020E-03 - 8.580E-02 mg As/kg bw./day). In silico analysis showed target genes connected with MIX and the development of: anemia - ACHE, GSR, PARP1, TNF; thrombocytosis – JAK2, CALR, MPL, THPO; hematological diseases - FAS and ALAD. The main extracted pathways for anemia were related to apoptosis and oxidative stress; for thrombocytosis were signaling pathways of Jak-STAT and TPO. Changes in miRNAs and transcription factors enabled the mode of action (MoA) development based on the obtained results, contributing to mechanistic understanding and hematological risk related to MIX exposure.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Integrative investigation of hematotoxic effects induced by low doses of
lead, cadmium, mercury and arsenic mixture: In vivo and in silico approach",
volume = "930",
doi = "10.1016/j.scitotenv.2024.172608, 2-s2.0-85191489918"
}

Živančević, K., Živanović, J., Baralić, K., Božić, D., Marić, Đ., Vukelić, D., Antonijević-Miljaković, E., Buha-Đorđević, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). Integrative investigation of hematotoxic effects induced by low doses of
lead, cadmium, mercury and arsenic mixture: In vivo and in silico approach. in Science of the Total Environment
Elsevier B.V.., 930.
https://doi.org/10.1016/j.scitotenv.2024.172608

Živančević K, Živanović J, Baralić K, Božić D, Marić Đ, Vukelić D, Antonijević-Miljaković E, Buha-Đorđević A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Integrative investigation of hematotoxic effects induced by low doses of
lead, cadmium, mercury and arsenic mixture: In vivo and in silico approach. in Science of the Total Environment. 2024;930.
doi:10.1016/j.scitotenv.2024.172608 .

Živančević, Katarina, Živanović, Jovana, Baralić, Katarina, Božić, Dragica, Marić, Đurđica, Vukelić, Dragana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Integrative investigation of hematotoxic effects induced by low doses of
lead, cadmium, mercury and arsenic mixture: In vivo and in silico approach" in Science of the Total Environment, 930 (2024),
https://doi.org/10.1016/j.scitotenv.2024.172608 . .

TY  - JOUR
AU  - Kovačević, Milena
AU  - Ivanović, Nevena
AU  - Protić, Ana
AU  - Milenković, Danijela
AU  - Mandinić, Zoran
AU  - Puzović, Dragana
AU  - Bajčetić, Miloš
AU  - Popadić, Dušan
AU  - Parojčić, Jelena
AU  - Malenović, Anđelija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5596
AB  - Digital transformation in education and relevant calls for action are recognised global priorities aimed to support education and training in the digital age. The experiences from the emergency remote teaching during the COVID-19 pandemic and recent research findings reveal relevant advantages, challenges, as well as different students and teaching staff perspectives which should be carefully considered and integrated into institutional strategies and policies to improve and enrich students’ learning experience. The present study aimed to explore health sciences students' perspectives on online teaching and learning (T&L) in four domains—Satisfaction, Motivation, Interaction and Challenges, and to identify the preferred T&L models or tools. A total of 1,041 responses were collected, including 476 dental medicine (45.7%), 399 pharmacy (38.3%), and 166 medical students (15.9%). Overall Satisfaction was quite high (4.0 ± 1.2; out of 5), followed by satisfactory Interaction (3.4 ± 1.4), whereas Motivation was quite low on average (3.0 ± 1.5). Challenges in online T&L were not markedly expressed (3.0 ± 1.6). The majority of students (46.6%) opted for the blended model as the preferred T&L model. Moodle lessons were perceived as the most useful online T&L tool (45.5% of students), followed by video materials (32.2%). Other T&L tools which require more active student's role, such as short test/quiz, and group or team assignment were declared as less favourable. The findings from the current study may contribute to raising the awareness of academic staff and higher institutions management about the student attitudes and needs related to online T&L and inform institutional planning, decision-making and policy development.
PB  - John Wiley & Sons Ltd
T2  - European Journal of Education
T1  - Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic
DO  - 10.1111/ejed.12660
ER  - 

@article{
author = "Kovačević, Milena and Ivanović, Nevena and Protić, Ana and Milenković, Danijela and Mandinić, Zoran and Puzović, Dragana and Bajčetić, Miloš and Popadić, Dušan and Parojčić, Jelena and Malenović, Anđelija",
year = "2024",
abstract = "Digital transformation in education and relevant calls for action are recognised global priorities aimed to support education and training in the digital age. The experiences from the emergency remote teaching during the COVID-19 pandemic and recent research findings reveal relevant advantages, challenges, as well as different students and teaching staff perspectives which should be carefully considered and integrated into institutional strategies and policies to improve and enrich students’ learning experience. The present study aimed to explore health sciences students' perspectives on online teaching and learning (T&L) in four domains—Satisfaction, Motivation, Interaction and Challenges, and to identify the preferred T&L models or tools. A total of 1,041 responses were collected, including 476 dental medicine (45.7%), 399 pharmacy (38.3%), and 166 medical students (15.9%). Overall Satisfaction was quite high (4.0 ± 1.2; out of 5), followed by satisfactory Interaction (3.4 ± 1.4), whereas Motivation was quite low on average (3.0 ± 1.5). Challenges in online T&L were not markedly expressed (3.0 ± 1.6). The majority of students (46.6%) opted for the blended model as the preferred T&L model. Moodle lessons were perceived as the most useful online T&L tool (45.5% of students), followed by video materials (32.2%). Other T&L tools which require more active student's role, such as short test/quiz, and group or team assignment were declared as less favourable. The findings from the current study may contribute to raising the awareness of academic staff and higher institutions management about the student attitudes and needs related to online T&L and inform institutional planning, decision-making and policy development.",
publisher = "John Wiley & Sons Ltd",
journal = "European Journal of Education",
title = "Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic",
doi = "10.1111/ejed.12660"
}

Kovačević, M., Ivanović, N., Protić, A., Milenković, D., Mandinić, Z., Puzović, D., Bajčetić, M., Popadić, D., Parojčić, J.,& Malenović, A.. (2024). Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic. in European Journal of Education
John Wiley & Sons Ltd..
https://doi.org/10.1111/ejed.12660

Kovačević M, Ivanović N, Protić A, Milenković D, Mandinić Z, Puzović D, Bajčetić M, Popadić D, Parojčić J, Malenović A. Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic. in European Journal of Education. 2024;.
doi:10.1111/ejed.12660 .

Kovačević, Milena, Ivanović, Nevena, Protić, Ana, Milenković, Danijela, Mandinić, Zoran, Puzović, Dragana, Bajčetić, Miloš, Popadić, Dušan, Parojčić, Jelena, Malenović, Anđelija, "Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic" in European Journal of Education (2024),
https://doi.org/10.1111/ejed.12660 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljević, Milica
AU  - Janković, Radmila
AU  - Đuretić, Jasmina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5626
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
PB  - MDPI
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Biljana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljević, Milica and Janković, Radmila and Đuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.",
publisher = "MDPI",
journal = "Biomedicines",
title = "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljević, M., Janković, R.,& Đuretić, J.. (2024). NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljević M, Janković R, Đuretić J. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4).
doi:10.3390/biomedicines12040717 .

Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024),
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Živančević, Katarina
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Marić, Đurđica
AU  - Kotur-Stevuljević, Jelena
AU  - Ivanišević, Jasmina
AU  - Savić, Miroslav
AU  - Batinić, Bojan
AU  - Janković, Radmila
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5614
AB  - Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.
PB  - Elsevier Inc.
T2  - Environmental Research
T1  - Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach
VL  - 252
IS  - 1
SP  - 118680
DO  - 10.1016/j.envres.2024.118680
ER  - 

@article{
author = "Živančević, Katarina and Baralić, Katarina and Vukelić, Dragana and Marić, Đurđica and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Savić, Miroslav and Batinić, Bojan and Janković, Radmila and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.",
publisher = "Elsevier Inc.",
journal = "Environmental Research",
title = "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach",
volume = "252",
number = "1",
pages = "118680",
doi = "10.1016/j.envres.2024.118680"
}

Živančević, K., Baralić, K., Vukelić, D., Marić, Đ., Kotur-Stevuljević, J., Ivanišević, J., Savić, M., Batinić, B., Janković, R., Buha-Đorđević, A., Antonijević-Miljaković, E., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research
Elsevier Inc.., 252(1), 118680.
https://doi.org/10.1016/j.envres.2024.118680

Živančević K, Baralić K, Vukelić D, Marić Đ, Kotur-Stevuljević J, Ivanišević J, Savić M, Batinić B, Janković R, Buha-Đorđević A, Antonijević-Miljaković E, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research. 2024;252(1):118680.
doi:10.1016/j.envres.2024.118680 .

Živančević, Katarina, Baralić, Katarina, Vukelić, Dragana, Marić, Đurđica, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Savić, Miroslav, Batinić, Bojan, Janković, Radmila, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach" in Environmental Research, 252, no. 1 (2024):118680,
https://doi.org/10.1016/j.envres.2024.118680 . .

TY  - JOUR
AU  - Stević, Ivana
AU  - Janković, Slobodan M.
AU  - Milošević-Georgiev, Andrijana
AU  - Marinković, Valentina
AU  - Lakić, Dragana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5618
AB  - Serious hematological adverse drug reactions (HADRs) may lead to or prolong hospitalization
and even cause death. The aim of this study was to determine the regulatory factors associated
with HADRs caused by drugs that were authorized up to July 2023 by the European Medicines
Agency (EMA) and to evaluate the frequency of HADRs. Using a cross-sectional approach, the type
and frequency of HADRs were collected from the Summaries of Product Characteristics of Drugs
Authorized by the EMA and analyzed within proprietary, nonproprietary, and biosimilar/biological
frameworks. Multivariate statistical analysis was used to investigate the associations of generic
status, biosimilar status, conditional approval, exceptional circumstances, accelerated assessment,
orphan drug status, years on the market, administration route, and inclusion on the Essential
Medicines List (EML) with HADRs. In total, 54.78% of proprietary drugs were associated with HADRs
at any frequency, while anemia, leucopenia, and thrombocytopenia were observed in approximately
36% of the patients. The predictors of any HADR, anemia, and thrombocytopenia of any frequency
are generic status, biosimilar status, and inclusion on the EML, while the only protective factor is the
administration route. Biosimilars and their originator biologicals have similar frequencies of HADRs;
the only exception is somatropin. Knowledge of the regulatory factors associated with HADRs could
help clinicians address monitoring issues when new drugs are introduced for the treatment of patients.
PB  - Nature Portfolio
T2  - Scientific Reports
T1  - Factors associated with hematological adverse reactions of drugs authorized via the centralized procedure
VL  - 14
IS  - 1
SP  - 9074
DO  - 10.1038/s41598-024-59710-3
ER  - 

@article{
author = "Stević, Ivana and Janković, Slobodan M. and Milošević-Georgiev, Andrijana and Marinković, Valentina and Lakić, Dragana",
year = "2024",
abstract = "Serious hematological adverse drug reactions (HADRs) may lead to or prolong hospitalization
and even cause death. The aim of this study was to determine the regulatory factors associated
with HADRs caused by drugs that were authorized up to July 2023 by the European Medicines
Agency (EMA) and to evaluate the frequency of HADRs. Using a cross-sectional approach, the type
and frequency of HADRs were collected from the Summaries of Product Characteristics of Drugs
Authorized by the EMA and analyzed within proprietary, nonproprietary, and biosimilar/biological
frameworks. Multivariate statistical analysis was used to investigate the associations of generic
status, biosimilar status, conditional approval, exceptional circumstances, accelerated assessment,
orphan drug status, years on the market, administration route, and inclusion on the Essential
Medicines List (EML) with HADRs. In total, 54.78% of proprietary drugs were associated with HADRs
at any frequency, while anemia, leucopenia, and thrombocytopenia were observed in approximately
36% of the patients. The predictors of any HADR, anemia, and thrombocytopenia of any frequency
are generic status, biosimilar status, and inclusion on the EML, while the only protective factor is the
administration route. Biosimilars and their originator biologicals have similar frequencies of HADRs;
the only exception is somatropin. Knowledge of the regulatory factors associated with HADRs could
help clinicians address monitoring issues when new drugs are introduced for the treatment of patients.",
publisher = "Nature Portfolio",
journal = "Scientific Reports",
title = "Factors associated with hematological adverse reactions of drugs authorized via the centralized procedure",
volume = "14",
number = "1",
pages = "9074",
doi = "10.1038/s41598-024-59710-3"
}

Stević, I., Janković, S. M., Milošević-Georgiev, A., Marinković, V.,& Lakić, D.. (2024). Factors associated with hematological adverse reactions of drugs authorized via the centralized procedure. in Scientific Reports
Nature Portfolio., 14(1), 9074.
https://doi.org/10.1038/s41598-024-59710-3

Stević I, Janković SM, Milošević-Georgiev A, Marinković V, Lakić D. Factors associated with hematological adverse reactions of drugs authorized via the centralized procedure. in Scientific Reports. 2024;14(1):9074.
doi:10.1038/s41598-024-59710-3 .

Stević, Ivana, Janković, Slobodan M., Milošević-Georgiev, Andrijana, Marinković, Valentina, Lakić, Dragana, "Factors associated with hematological adverse reactions of drugs authorized via the centralized procedure" in Scientific Reports, 14, no. 1 (2024):9074,
https://doi.org/10.1038/s41598-024-59710-3 . .

TY  - JOUR
AU  - Draganov, Ivana
AU  - Drndarević, Aneta
AU  - Kovačević, Milena
AU  - Miljković, Branislava
AU  - Vuksanović, Miljanka
AU  - Janković, Aleksandar
AU  - Kalaba, Ana
AU  - Vezmar-Kovačević, Sandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5604
AB  - Drug-related hospitalizations pose a significant burden to the health-care system. The aim
was to investigate the prevalence of drug-related problems (DRPs) and their association with hospital
admissions in five internal medicine wards. The study included patients admitted to the nephrology,
cardiology, gastroenterology, endocrinology, and geriatric wards. The Pharmaceutical Care Network
Europe classification V9.1 was used for identifying DRPs. In total, 535 patients participated in the study.
We identified 954 DRPs (range 1-7) in 80.7% of patients. Most DRPs were identified on the endocrinology,
cardiology, and geriatric wards, and they were associated with the efficacy of treatment (71.4%), adverse
drug events (10.2%), and unnecessary drug treatment (18.4%). DRPs were associated with the cause of
hospitalization in 74.4% of patients on the nephrology ward, 60.1% and 60.6% of patients on the cardiology
and endocrinology wards, respectively, whereas this number was lower on the geriatric and gastroenterology
wards (26.9% and 8.9%, respectively). Suboptimal drug treatment due to medication omissions was often
associated with the potential cause of hospital admission. Focusing on patients with specific diseases and
DRPs, rather than reducing the number of medications in primary care, may be potentially rational in an
attempt to reduce drug-related hospitalizations.
PB  - Polish Pharmaceutical Society
T2  - Acta Poloniae Pharmaceutica - Drug Research
T1  - Drug-Related Problems Prior to Hospitalization on Internal Medicine Wards
VL  - 81
IS  - 1
SP  - 145
EP  - 154
DO  - 10.32383/appdr/182840
ER  - 

@article{
author = "Draganov, Ivana and Drndarević, Aneta and Kovačević, Milena and Miljković, Branislava and Vuksanović, Miljanka and Janković, Aleksandar and Kalaba, Ana and Vezmar-Kovačević, Sandra",
year = "2024",
abstract = "Drug-related hospitalizations pose a significant burden to the health-care system. The aim
was to investigate the prevalence of drug-related problems (DRPs) and their association with hospital
admissions in five internal medicine wards. The study included patients admitted to the nephrology,
cardiology, gastroenterology, endocrinology, and geriatric wards. The Pharmaceutical Care Network
Europe classification V9.1 was used for identifying DRPs. In total, 535 patients participated in the study.
We identified 954 DRPs (range 1-7) in 80.7% of patients. Most DRPs were identified on the endocrinology,
cardiology, and geriatric wards, and they were associated with the efficacy of treatment (71.4%), adverse
drug events (10.2%), and unnecessary drug treatment (18.4%). DRPs were associated with the cause of
hospitalization in 74.4% of patients on the nephrology ward, 60.1% and 60.6% of patients on the cardiology
and endocrinology wards, respectively, whereas this number was lower on the geriatric and gastroenterology
wards (26.9% and 8.9%, respectively). Suboptimal drug treatment due to medication omissions was often
associated with the potential cause of hospital admission. Focusing on patients with specific diseases and
DRPs, rather than reducing the number of medications in primary care, may be potentially rational in an
attempt to reduce drug-related hospitalizations.",
publisher = "Polish Pharmaceutical Society",
journal = "Acta Poloniae Pharmaceutica - Drug Research",
title = "Drug-Related Problems Prior to Hospitalization on Internal Medicine Wards",
volume = "81",
number = "1",
pages = "145-154",
doi = "10.32383/appdr/182840"
}

Draganov, I., Drndarević, A., Kovačević, M., Miljković, B., Vuksanović, M., Janković, A., Kalaba, A.,& Vezmar-Kovačević, S.. (2024). Drug-Related Problems Prior to Hospitalization on Internal Medicine Wards. in Acta Poloniae Pharmaceutica - Drug Research
Polish Pharmaceutical Society., 81(1), 145-154.
https://doi.org/10.32383/appdr/182840

Draganov I, Drndarević A, Kovačević M, Miljković B, Vuksanović M, Janković A, Kalaba A, Vezmar-Kovačević S. Drug-Related Problems Prior to Hospitalization on Internal Medicine Wards. in Acta Poloniae Pharmaceutica - Drug Research. 2024;81(1):145-154.
doi:10.32383/appdr/182840 .

Draganov, Ivana, Drndarević, Aneta, Kovačević, Milena, Miljković, Branislava, Vuksanović, Miljanka, Janković, Aleksandar, Kalaba, Ana, Vezmar-Kovačević, Sandra, "Drug-Related Problems Prior to Hospitalization on Internal Medicine Wards" in Acta Poloniae Pharmaceutica - Drug Research, 81, no. 1 (2024):145-154,
https://doi.org/10.32383/appdr/182840 . .

TY  - JOUR
AU  - Obradović, Darija
AU  - Savić, Jelena
AU  - Joksimović, Jovana
AU  - Marković, Bojan
AU  - Vujić, Zorica
AU  - Lazović, Saša
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5549
AB  - Abstract: The retention profile of six serotonin receptor ligands such as arylpiperazines (aripiprazole, ziprasidone, risperidone), and benzothiazepine derivatives (olanzapine, mianserin, quetiapine), was investigated on C8 alkyl and pentafluorophenylpropyl stationary phases. The experimental design methodology was used to define their retention behavior and achieve acceptable separation results. Both phases showed satisfactory selectivity for all compounds. The optimal separation among the structurally related arylpiperazines and benzothiazepines was found with the C8 phase at 25°C, using acetonitrile (40%, v/v) and 20 mM ammonium acetate as a mobile phase modifier. The selectivity and sensitivity performances of the selected C8 system were discussed considering the separation of aripiprazole and its related compounds. In addition, the specified conditions were validated for determining aripiprazole in pharmaceutical dosage forms. The developed reversed-phase high-performance liquid chromatography method can be successfully used for the rapid chromatographic profiling of serotonin receptor ligands and related analogs, providing increased selectivity during pharmaceutical analysis.
PB  - Springer
T2  - Journal of Analytical Chemistry
T1  - Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds
VL  - 79
IS  - 1
SP  - 95
EP  - 104
DO  - 10.1134/S1061934824010076
ER  - 

@article{
author = "Obradović, Darija and Savić, Jelena and Joksimović, Jovana and Marković, Bojan and Vujić, Zorica and Lazović, Saša",
year = "2024",
abstract = "Abstract: The retention profile of six serotonin receptor ligands such as arylpiperazines (aripiprazole, ziprasidone, risperidone), and benzothiazepine derivatives (olanzapine, mianserin, quetiapine), was investigated on C8 alkyl and pentafluorophenylpropyl stationary phases. The experimental design methodology was used to define their retention behavior and achieve acceptable separation results. Both phases showed satisfactory selectivity for all compounds. The optimal separation among the structurally related arylpiperazines and benzothiazepines was found with the C8 phase at 25°C, using acetonitrile (40%, v/v) and 20 mM ammonium acetate as a mobile phase modifier. The selectivity and sensitivity performances of the selected C8 system were discussed considering the separation of aripiprazole and its related compounds. In addition, the specified conditions were validated for determining aripiprazole in pharmaceutical dosage forms. The developed reversed-phase high-performance liquid chromatography method can be successfully used for the rapid chromatographic profiling of serotonin receptor ligands and related analogs, providing increased selectivity during pharmaceutical analysis.",
publisher = "Springer",
journal = "Journal of Analytical Chemistry",
title = "Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds",
volume = "79",
number = "1",
pages = "95-104",
doi = "10.1134/S1061934824010076"
}

Obradović, D., Savić, J., Joksimović, J., Marković, B., Vujić, Z.,& Lazović, S.. (2024). Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds. in Journal of Analytical Chemistry
Springer., 79(1), 95-104.
https://doi.org/10.1134/S1061934824010076

Obradović D, Savić J, Joksimović J, Marković B, Vujić Z, Lazović S. Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds. in Journal of Analytical Chemistry. 2024;79(1):95-104.
doi:10.1134/S1061934824010076 .

Obradović, Darija, Savić, Jelena, Joksimović, Jovana, Marković, Bojan, Vujić, Zorica, Lazović, Saša, "Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds" in Journal of Analytical Chemistry, 79, no. 1 (2024):95-104,
https://doi.org/10.1134/S1061934824010076 . .

TY  - JOUR
AU  - Gajić Bojić, Milica
AU  - Treven, Marco
AU  - Pandey, Kamal P
AU  - Tiruveedhula, Phani Babu V V N
AU  - Santrač, Anja
AU  - Đukanović, Đorđe
AU  - Vojinović, Nataša
AU  - Amidžić, Ljiljana
AU  - Škrbić, Ranko
AU  - Scholze, Petra
AU  - Ernst, Margot
AU  - Cook, James M
AU  - Savić, Miroslav
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5619
AB  - Hypotensive influences of benzodiazepines and other GABAA receptor ligands, recognized in clinical practice, seem to stem from the existence of “vascular” GABAA receptors in peripheral blood vessels, besides any mechanisms in the central and peripheral nervous systems. We aimed to further elucidate the vasodilatatory effects of ligands acting through GABAA receptors. Using immunohistochemistry, the rat aortic smooth muscle layer was found to express GABAA γ 2 and α1-5 subunit proteins. To confirm the role of “vascular” GABAA receptors, we investigated the vascular effects of standard benzodiazepines, mida-zolam, and flumazenil, as well as the novel compound MP-III-058. Using two-electrode voltage clamp electrophysiology and radioligand binding assays, MP-III-058 was found to have modest binding but substantial functional selectivity for α5β3γ 2 over other αxβ3γ 2 GABAA receptors. Tissue bath assays revealed comparable vasodilatory effects of MP-III-058 and midazo-lam, both of which at 100 μmol/L concentrations had efficacy similar to prazosin. Flumazenil exhibited weak vasoactivity per se, but significantly prevented the relaxant effects of midazolam and MP-III-058. These studies indicate the existence of functional GABAA receptors in the rat aorta, where ligands exert vasodilatory effects by positive modulation of the benzodiazepine binding site, suggesting the potential for further quest for leads with optimized pharmacokinetic properties as prospective adjuvant vasodilators.
PB  - Canadian Science Publishing
T2  - Canadian  Journal of Physiology and  Pharmacology
T1  - Vascular effects of midazolam, flumazenil, and a novel imidazobenzodiazepine MP-III-058 on isolated rat aorta
VL  - 102
IS  - 3
SP  - 206
EP  - 217
DO  - 10.1139/cjpp-2023-0285
ER  - 

@article{
author = "Gajić Bojić, Milica and Treven, Marco and Pandey, Kamal P and Tiruveedhula, Phani Babu V V N and Santrač, Anja and Đukanović, Đorđe and Vojinović, Nataša and Amidžić, Ljiljana and Škrbić, Ranko and Scholze, Petra and Ernst, Margot and Cook, James M and Savić, Miroslav",
year = "2024",
abstract = "Hypotensive influences of benzodiazepines and other GABAA receptor ligands, recognized in clinical practice, seem to stem from the existence of “vascular” GABAA receptors in peripheral blood vessels, besides any mechanisms in the central and peripheral nervous systems. We aimed to further elucidate the vasodilatatory effects of ligands acting through GABAA receptors. Using immunohistochemistry, the rat aortic smooth muscle layer was found to express GABAA γ 2 and α1-5 subunit proteins. To confirm the role of “vascular” GABAA receptors, we investigated the vascular effects of standard benzodiazepines, mida-zolam, and flumazenil, as well as the novel compound MP-III-058. Using two-electrode voltage clamp electrophysiology and radioligand binding assays, MP-III-058 was found to have modest binding but substantial functional selectivity for α5β3γ 2 over other αxβ3γ 2 GABAA receptors. Tissue bath assays revealed comparable vasodilatory effects of MP-III-058 and midazo-lam, both of which at 100 μmol/L concentrations had efficacy similar to prazosin. Flumazenil exhibited weak vasoactivity per se, but significantly prevented the relaxant effects of midazolam and MP-III-058. These studies indicate the existence of functional GABAA receptors in the rat aorta, where ligands exert vasodilatory effects by positive modulation of the benzodiazepine binding site, suggesting the potential for further quest for leads with optimized pharmacokinetic properties as prospective adjuvant vasodilators.",
publisher = "Canadian Science Publishing",
journal = "Canadian  Journal of Physiology and  Pharmacology",
title = "Vascular effects of midazolam, flumazenil, and a novel imidazobenzodiazepine MP-III-058 on isolated rat aorta",
volume = "102",
number = "3",
pages = "206-217",
doi = "10.1139/cjpp-2023-0285"
}

Gajić Bojić, M., Treven, M., Pandey, K. P., Tiruveedhula, P. B. V. V. N., Santrač, A., Đukanović, Đ., Vojinović, N., Amidžić, L., Škrbić, R., Scholze, P., Ernst, M., Cook, J. M.,& Savić, M.. (2024). Vascular effects of midazolam, flumazenil, and a novel imidazobenzodiazepine MP-III-058 on isolated rat aorta. in Canadian  Journal of Physiology and  Pharmacology
Canadian Science Publishing., 102(3), 206-217.
https://doi.org/10.1139/cjpp-2023-0285

Gajić Bojić M, Treven M, Pandey KP, Tiruveedhula PBVVN, Santrač A, Đukanović Đ, Vojinović N, Amidžić L, Škrbić R, Scholze P, Ernst M, Cook JM, Savić M. Vascular effects of midazolam, flumazenil, and a novel imidazobenzodiazepine MP-III-058 on isolated rat aorta. in Canadian  Journal of Physiology and  Pharmacology. 2024;102(3):206-217.
doi:10.1139/cjpp-2023-0285 .

Gajić Bojić, Milica, Treven, Marco, Pandey, Kamal P, Tiruveedhula, Phani Babu V V N, Santrač, Anja, Đukanović, Đorđe, Vojinović, Nataša, Amidžić, Ljiljana, Škrbić, Ranko, Scholze, Petra, Ernst, Margot, Cook, James M, Savić, Miroslav, "Vascular effects of midazolam, flumazenil, and a novel imidazobenzodiazepine MP-III-058 on isolated rat aorta" in Canadian  Journal of Physiology and  Pharmacology, 102, no. 3 (2024):206-217,
https://doi.org/10.1139/cjpp-2023-0285 . .

TY  - JOUR
AU  - Ćurčić, Vladimir
AU  - Olszewski, Mateusz
AU  - Maciejewska, Natalia
AU  - Višnjevac, Aleksandar
AU  - Srdić-Rajić, Tatjana
AU  - Dobričić, Vladimir
AU  - García-Sosa, Alfonso T.
AU  - Kokanov, Sanja B.
AU  - Araškov, Jovana B.
AU  - Silvestri, Romano
AU  - Schüle, Roland
AU  - Jung, Manfred
AU  - Nikolić, Milan
AU  - Filipović, Nenad R.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5298
AB  - Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.
PB  - John Wiley and Sons Inc
T2  - Archiv der Pharmazie
T1  - Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition
VL  - 357
IS  - 2
SP  - 2300426
DO  - 10.1002/ardp.202300426
ER  - 

@article{
author = "Ćurčić, Vladimir and Olszewski, Mateusz and Maciejewska, Natalia and Višnjevac, Aleksandar and Srdić-Rajić, Tatjana and Dobričić, Vladimir and García-Sosa, Alfonso T. and Kokanov, Sanja B. and Araškov, Jovana B. and Silvestri, Romano and Schüle, Roland and Jung, Manfred and Nikolić, Milan and Filipović, Nenad R.",
year = "2024",
abstract = "Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.",
publisher = "John Wiley and Sons Inc",
journal = "Archiv der Pharmazie",
title = "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition",
volume = "357",
number = "2",
pages = "2300426",
doi = "10.1002/ardp.202300426"
}

Ćurčić, V., Olszewski, M., Maciejewska, N., Višnjevac, A., Srdić-Rajić, T., Dobričić, V., García-Sosa, A. T., Kokanov, S. B., Araškov, J. B., Silvestri, R., Schüle, R., Jung, M., Nikolić, M.,& Filipović, N. R.. (2024). Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie
John Wiley and Sons Inc., 357(2), 2300426.
https://doi.org/10.1002/ardp.202300426

Ćurčić V, Olszewski M, Maciejewska N, Višnjevac A, Srdić-Rajić T, Dobričić V, García-Sosa AT, Kokanov SB, Araškov JB, Silvestri R, Schüle R, Jung M, Nikolić M, Filipović NR. Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie. 2024;357(2):2300426.
doi:10.1002/ardp.202300426 .

Ćurčić, Vladimir, Olszewski, Mateusz, Maciejewska, Natalia, Višnjevac, Aleksandar, Srdić-Rajić, Tatjana, Dobričić, Vladimir, García-Sosa, Alfonso T., Kokanov, Sanja B., Araškov, Jovana B., Silvestri, Romano, Schüle, Roland, Jung, Manfred, Nikolić, Milan, Filipović, Nenad R., "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition" in Archiv der Pharmazie, 357, no. 2 (2024):2300426,
https://doi.org/10.1002/ardp.202300426 . .

TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Jovanović-Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5613
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Jovanović-Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka-Aleksić, M., Jovanović-Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka-Aleksić M, Jovanović-Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Jovanović-Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - JOUR
AU  - Pešić, Deniel
AU  - Đukić, Mirjana
AU  - Stanojević, Ivan
AU  - Živković, Vladimir
AU  - Bolevich, Sergey
AU  - Bolevich, Stefani
AU  - Jakovljević, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5559
AB  - Background: The influence of homeostatically regulated physiological processes, including cardiorespiratory fitness (VO2max), on the response to physical stressors such as acclimatisation and marching, remains understudied. We aimed to investigate the effects of summer and winter acclimatisation and marching on cortisol levels and blood lactate, to gain insight into the role of these physiological processes in the stress response. Methods: Two groups of young Europeans, classified as poor (PCF; n=9) and good physical condition (GCF; n=21), based on a VO2max threshold of 40 mL O2/ kg/min, underwent 2-h march (6–7 km/h) in winter (5 ∘C) and summer (32 ∘C). Commercial tests, UniCel DxI Access Cortisol assay and EKF Biosen Clinic/GP assay were used for cortisol and lactate blood measurements (morning samples and those taken immediately after marches), respectively. Results: Basal cortisol levels were significantly higher at 5 °C than at 32 °C (PCF group: P=0.0079; cortisol dropped after the march at 5 °C in both groups, but increased at 32 °C only in PCF. Basal lactate levels were higher at 32 °C only in the GCF group (compared to PCF, P=0.0014) and post-marching (compared to basal values, P=0.0002). Conclusion: Cold exposure elicits a more significant stress response, based on higher basal cortisol levels, in individuals with worse physical fitness. Anaerobe metabolism prevails in participants with better physical fitness exposed to high ambient temperature based on increased basal and post-marching lactate levels. The study is significant for individual training/performance optimisation as it indicates the association between physiological stress responses and individual physical fitness levels.
AB  - Uvod: Uticaj homeostatski regulisanih fiziolo{kih procesa, uklju~uju}i kardiorespiratornu kondiciju (VO2max), na odgovor na fizi~ke stresore poput aklimatizacije i mar{i- ranja, ostaje nedovoljno prou~en. Cilj istra`ivanja je bio i da se ispita uticaj aklimatizacije i mar{iranja tokom leta i zime na nivoe kortizola i laktoze u krvi, pru`aju}i uvid u ulogu ovih fiziolo{kih procesa u odgovoru na stres. Metode: Dve grupe mladih Evropljana, klasifikovane kao osobe sa slabom (PCF; n=9) i dobrom fizi~kom kondicijom (GCF; n=21), na osnovu praga VO2max od 40 mL O 2/kg/min, su bile izlo`ene dvosatnom mar{u (6–7 km/h) u dva navrata, zimi na 5 °C i leti, na 32 °C. U uzorcima krvi (jutarnji i neposredno nakon mar{a) su odre|ivani nivoi kortizola i laktata komercijalnim testovima, UniCel DxI Access Cortisol i EKF Biosen Clinic/GP. Rezultati: Bazalni nivoi kortizola su bili zna~ajno vi{i na 5 °C nego na 32 °C (u PCF grupi: P=0,0079); nakon mar{i- ranja na 5 °C kortizol opada u obe grupe a pove}an je na 32 °C samo u PCF grupi. Bazalni nivoi laktoze bili su vi{i na 32 °C samo u GCF grupi (porede}i sa PCF grupom, P=0,0014) i nakon mar{iranja (porede}i sa bazalnim vred- nostima, P=0,0002).
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches
T1  - Kardiorespiratorni fitnes i njegov značaj u reakcijama kortizola i laktata prilikom zimskih i letnjih marševa
VL  - 43
IS  - 1
SP  - 72
EP  - 85
DO  - 10.5937/jomb0-44369
ER  - 

@article{
author = "Pešić, Deniel and Đukić, Mirjana and Stanojević, Ivan and Živković, Vladimir and Bolevich, Sergey and Bolevich, Stefani and Jakovljević, Vladimir",
year = "2024",
abstract = "Background: The influence of homeostatically regulated physiological processes, including cardiorespiratory fitness (VO2max), on the response to physical stressors such as acclimatisation and marching, remains understudied. We aimed to investigate the effects of summer and winter acclimatisation and marching on cortisol levels and blood lactate, to gain insight into the role of these physiological processes in the stress response. Methods: Two groups of young Europeans, classified as poor (PCF; n=9) and good physical condition (GCF; n=21), based on a VO2max threshold of 40 mL O2/ kg/min, underwent 2-h march (6–7 km/h) in winter (5 ∘C) and summer (32 ∘C). Commercial tests, UniCel DxI Access Cortisol assay and EKF Biosen Clinic/GP assay were used for cortisol and lactate blood measurements (morning samples and those taken immediately after marches), respectively. Results: Basal cortisol levels were significantly higher at 5 °C than at 32 °C (PCF group: P=0.0079; cortisol dropped after the march at 5 °C in both groups, but increased at 32 °C only in PCF. Basal lactate levels were higher at 32 °C only in the GCF group (compared to PCF, P=0.0014) and post-marching (compared to basal values, P=0.0002). Conclusion: Cold exposure elicits a more significant stress response, based on higher basal cortisol levels, in individuals with worse physical fitness. Anaerobe metabolism prevails in participants with better physical fitness exposed to high ambient temperature based on increased basal and post-marching lactate levels. The study is significant for individual training/performance optimisation as it indicates the association between physiological stress responses and individual physical fitness levels., Uvod: Uticaj homeostatski regulisanih fiziolo{kih procesa, uklju~uju}i kardiorespiratornu kondiciju (VO2max), na odgovor na fizi~ke stresore poput aklimatizacije i mar{i- ranja, ostaje nedovoljno prou~en. Cilj istra`ivanja je bio i da se ispita uticaj aklimatizacije i mar{iranja tokom leta i zime na nivoe kortizola i laktoze u krvi, pru`aju}i uvid u ulogu ovih fiziolo{kih procesa u odgovoru na stres. Metode: Dve grupe mladih Evropljana, klasifikovane kao osobe sa slabom (PCF; n=9) i dobrom fizi~kom kondicijom (GCF; n=21), na osnovu praga VO2max od 40 mL O 2/kg/min, su bile izlo`ene dvosatnom mar{u (6–7 km/h) u dva navrata, zimi na 5 °C i leti, na 32 °C. U uzorcima krvi (jutarnji i neposredno nakon mar{a) su odre|ivani nivoi kortizola i laktata komercijalnim testovima, UniCel DxI Access Cortisol i EKF Biosen Clinic/GP. Rezultati: Bazalni nivoi kortizola su bili zna~ajno vi{i na 5 °C nego na 32 °C (u PCF grupi: P=0,0079); nakon mar{i- ranja na 5 °C kortizol opada u obe grupe a pove}an je na 32 °C samo u PCF grupi. Bazalni nivoi laktoze bili su vi{i na 32 °C samo u GCF grupi (porede}i sa PCF grupom, P=0,0014) i nakon mar{iranja (porede}i sa bazalnim vred- nostima, P=0,0002).",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches, Kardiorespiratorni fitnes i njegov značaj u reakcijama kortizola i laktata prilikom zimskih i letnjih marševa",
volume = "43",
number = "1",
pages = "72-85",
doi = "10.5937/jomb0-44369"
}

Pešić, D., Đukić, M., Stanojević, I., Živković, V., Bolevich, S., Bolevich, S.,& Jakovljević, V.. (2024). Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 43(1), 72-85.
https://doi.org/10.5937/jomb0-44369

Pešić D, Đukić M, Stanojević I, Živković V, Bolevich S, Bolevich S, Jakovljević V. Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches. in Journal of Medical Biochemistry. 2024;43(1):72-85.
doi:10.5937/jomb0-44369 .

Pešić, Deniel, Đukić, Mirjana, Stanojević, Ivan, Živković, Vladimir, Bolevich, Sergey, Bolevich, Stefani, Jakovljević, Vladimir, "Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches" in Journal of Medical Biochemistry, 43, no. 1 (2024):72-85,
https://doi.org/10.5937/jomb0-44369 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5418
AB  - The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.
PB  - MDPI
T2  - Separations
T1  - Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach
VL  - 11
IS  - 1
SP  - 14
DO  - 10.3390/separations11010014
ER  - 

@article{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.",
publisher = "MDPI",
journal = "Separations",
title = "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach",
volume = "11",
number = "1",
pages = "14",
doi = "10.3390/separations11010014"
}

Ušjak, L., Niketić, M.,& Petrović, S.. (2024). Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations
MDPI., 11(1), 14.
https://doi.org/10.3390/separations11010014

Ušjak L, Niketić M, Petrović S. Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations. 2024;11(1):14.
doi:10.3390/separations11010014 .

Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach" in Separations, 11, no. 1 (2024):14,
https://doi.org/10.3390/separations11010014 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Savić, Jelena
AU  - Tomašić, Tihomir
AU  - Durcik, Martina
AU  - Zidar, Nace
AU  - Peterlin-Mašič, Lucija
AU  - Ilaš, Janez
AU  - Kikelj, Danijel
AU  - Čudina, Olivera
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5431
AB  - Bacterial DNA gyrase and topoisomerase IV control the topological state of DNA during replication
and represent important antibacterial drug targets. To be successful as drug candidates, newly synthesized compounds must possess optimal lipophilicity, which enables efficient delivery to the site of action. In this study, retention behavior of twenty-three previously synthesized dual DNA gyrase and topoisomerase IV inhibitors was tested in RP-HPLC system, consisting of C8 column and acetonitrile/phosphate buffer (pH 5.5 and pH 7.4) mobile phase. logD was calculated at both pH values and the best correlation with logD was obtained for retention parameter φ0, indicating that this RP-HPLC system could be used as an alternative to the shake-flask determination of lipophilicity. Subsequent QSRR analysis revealed that intrinsic lipophilicity (logP) and molecular weight (bcutm13) have a positive, while solubility (bcutp3) has a negative influence on this  retention parameter.
PB  - Akadémiai Kiadó
T2  - Acta Chromatographica
T1  - High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors
VL  - 36
IS  - 1
SP  - 45
EP  - 51
DO  - 10.1556/1326.2022.01096
ER  - 

@article{
author = "Dobričić, Vladimir and Savić, Jelena and Tomašić, Tihomir and Durcik, Martina and Zidar, Nace and Peterlin-Mašič, Lucija and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2024",
abstract = "Bacterial DNA gyrase and topoisomerase IV control the topological state of DNA during replication
and represent important antibacterial drug targets. To be successful as drug candidates, newly synthesized compounds must possess optimal lipophilicity, which enables efficient delivery to the site of action. In this study, retention behavior of twenty-three previously synthesized dual DNA gyrase and topoisomerase IV inhibitors was tested in RP-HPLC system, consisting of C8 column and acetonitrile/phosphate buffer (pH 5.5 and pH 7.4) mobile phase. logD was calculated at both pH values and the best correlation with logD was obtained for retention parameter φ0, indicating that this RP-HPLC system could be used as an alternative to the shake-flask determination of lipophilicity. Subsequent QSRR analysis revealed that intrinsic lipophilicity (logP) and molecular weight (bcutm13) have a positive, while solubility (bcutp3) has a negative influence on this  retention parameter.",
publisher = "Akadémiai Kiadó",
journal = "Acta Chromatographica",
title = "High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors",
volume = "36",
number = "1",
pages = "45-51",
doi = "10.1556/1326.2022.01096"
}

Dobričić, V., Savić, J., Tomašić, T., Durcik, M., Zidar, N., Peterlin-Mašič, L., Ilaš, J., Kikelj, D.,& Čudina, O.. (2024). High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors. in Acta Chromatographica
Akadémiai Kiadó., 36(1), 45-51.
https://doi.org/10.1556/1326.2022.01096

Dobričić V, Savić J, Tomašić T, Durcik M, Zidar N, Peterlin-Mašič L, Ilaš J, Kikelj D, Čudina O. High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors. in Acta Chromatographica. 2024;36(1):45-51.
doi:10.1556/1326.2022.01096 .

Dobričić, Vladimir, Savić, Jelena, Tomašić, Tihomir, Durcik, Martina, Zidar, Nace, Peterlin-Mašič, Lucija, Ilaš, Janez, Kikelj, Danijel, Čudina, Olivera, "High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors" in Acta Chromatographica, 36, no. 1 (2024):45-51,
https://doi.org/10.1556/1326.2022.01096 . .

TY  - JOUR
AU  - Milošević, Tamara
AU  - Sopić, Miron
AU  - Vekić, Jelena
AU  - Guzonjić, Azra
AU  - Vujčić, Sanja
AU  - Pešić, Snežana
AU  - Miljković-Trailović, Milica
AU  - Naumović, Radomir
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5414
AB  - Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.
PB  - Springer Science and Business Media B.V.
T2  - International Urology and Nephrology
T1  - The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients
VL  - 56
IS  - 2
SP  - 615
EP  - 623
DO  - 10.1007/s11255-023-03696-w
ER  - 

@article{
author = "Milošević, Tamara and Sopić, Miron and Vekić, Jelena and Guzonjić, Azra and Vujčić, Sanja and Pešić, Snežana and Miljković-Trailović, Milica and Naumović, Radomir and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "International Urology and Nephrology",
title = "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients",
volume = "56",
number = "2",
pages = "615-623",
doi = "10.1007/s11255-023-03696-w"
}

Milošević, T., Sopić, M., Vekić, J., Guzonjić, A., Vujčić, S., Pešić, S., Miljković-Trailović, M., Naumović, R.,& Kotur-Stevuljević, J.. (2024). The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology
Springer Science and Business Media B.V.., 56(2), 615-623.
https://doi.org/10.1007/s11255-023-03696-w

Milošević T, Sopić M, Vekić J, Guzonjić A, Vujčić S, Pešić S, Miljković-Trailović M, Naumović R, Kotur-Stevuljević J. The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology. 2024;56(2):615-623.
doi:10.1007/s11255-023-03696-w .

Milošević, Tamara, Sopić, Miron, Vekić, Jelena, Guzonjić, Azra, Vujčić, Sanja, Pešić, Snežana, Miljković-Trailović, Milica, Naumović, Radomir, Kotur-Stevuljević, Jelena, "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients" in International Urology and Nephrology, 56, no. 2 (2024):615-623,
https://doi.org/10.1007/s11255-023-03696-w . .