TY  - JOUR
AU  - Baralić, Katarina
AU  - Božović, Predrag
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5680
AB  - Even at low levels, exposure to ionising radiation can lead to eye damage. However, the underlying molecular mechanisms are not yet fully
understood. We aimed to address this gap with a comprehensive in silico approach to the issue. For this purpose we relied on the Comparative
Toxicogenomics Database (CTD), ToppGene Suite, Cytoscape, GeneMANIA, and Metascape to identify six key regulator genes associated
with radiation-induced eye damage (ATM, CRYAB, SIRT1, TGFB1, TREX1, and YAP1), all of which have physical interactions. Some
of the identified molecular functions revolve around DNA repair mechanisms, while others are involved in protein binding, enzymatic
activities, metabolic processes, and post-translational protein modifications. The biological processes are mostly centred on response to
DNA damage, the p53 signalling pathway in particular. We identified a significant role of several miRNAs, such as hsa-miR-183 and hsamiR-589, in the mechanisms behind ionising radiation-induced eye injuries. Our study offers a valuable method for gaining deeper insights
into the adverse effects of radiation exposure.
PB  - Institute for Medical Research and Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining
VL  - 75
IS  - 2
SP  - 91
EP  - 101
DO  - 10.2478/aiht-2024-75-3817
ER  - 

@article{
author = "Baralić, Katarina and Božović, Predrag and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Even at low levels, exposure to ionising radiation can lead to eye damage. However, the underlying molecular mechanisms are not yet fully
understood. We aimed to address this gap with a comprehensive in silico approach to the issue. For this purpose we relied on the Comparative
Toxicogenomics Database (CTD), ToppGene Suite, Cytoscape, GeneMANIA, and Metascape to identify six key regulator genes associated
with radiation-induced eye damage (ATM, CRYAB, SIRT1, TGFB1, TREX1, and YAP1), all of which have physical interactions. Some
of the identified molecular functions revolve around DNA repair mechanisms, while others are involved in protein binding, enzymatic
activities, metabolic processes, and post-translational protein modifications. The biological processes are mostly centred on response to
DNA damage, the p53 signalling pathway in particular. We identified a significant role of several miRNAs, such as hsa-miR-183 and hsamiR-589, in the mechanisms behind ionising radiation-induced eye injuries. Our study offers a valuable method for gaining deeper insights
into the adverse effects of radiation exposure.",
publisher = "Institute for Medical Research and Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining",
volume = "75",
number = "2",
pages = "91-101",
doi = "10.2478/aiht-2024-75-3817"
}

Baralić, K., Božović, P.,& Đukić-Ćosić, D.. (2024). Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Institute for Medical Research and Occupational Health, Zagreb., 75(2), 91-101.
https://doi.org/10.2478/aiht-2024-75-3817

Baralić K, Božović P, Đukić-Ćosić D. Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(2):91-101.
doi:10.2478/aiht-2024-75-3817 .

Baralić, Katarina, Božović, Predrag, Đukić-Ćosić, Danijela, "Deciphering the molecular landscape of ionising radiation-induced eye damage with the help of genomic data mining" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 2 (2024):91-101,
https://doi.org/10.2478/aiht-2024-75-3817 . .

TY  - JOUR
AU  - Živanović, Jovana
AU  - Baralić, Katarina
AU  - Živančević, Katarina
AU  - Božić, Dragica
AU  - Marić, Đurđica
AU  - Antonijević Miljaković, Evica
AU  - Buha Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5681
AB  - COVID-19 can cause a range of complications, including cardiovascular, renal, and/or respiratory insufficiencies, yet little is known of
its potential effects in persons exposed to toxic metals. The aim of this study was to answer this question with in silico toxicogenomic
methods that can provide molecular insights into COVID-19 complications owed to exposure to arsenic, cadmium, lead, mercury, nickel,
and chromium. For this purpose we relied on the Comparative Toxicogenomic Database (CTD), GeneMANIA, and ToppGene Suite
portal and identified a set of five common genes (IL1B, CXCL8, IL6, IL10, TNF) for the six metals and COVID-19, all of which code
for pro-inflammatory and anti-inflammatory cytokines. The list was expanded with additional 20 related genes. Physical interactions are
the most common between the genes affected by the six metals (77.64 %), while the dominant interaction between the genes affected by
each metal separately is co-expression (As 56.35 %, Cd 64.07 %, Pb 71.5 %, Hg 81.91 %, Ni 64.28 %, Cr 88.51 %). Biological processes,
molecular functions, and pathways in which these 25 genes participate are closely related to cytokines and cytokine storm implicated in
the development of COVID-19 complications. In other words, our findings confirm that exposure to toxic metals, alone or in combinations,
might escalate COVID-19 severity.
AB  - COVID-19 može izazvati niz komplikacija, uključujući kardiovaskularnu, bubrežnu i/ili respiratornu insuficijenciju, ali se malo zna o
njegovim potencijalnim učincima u osoba koje su izložene toksičnim metalima. Cilj ovog istraživanja bio je odgovoriti na to pitanje pomoću
in silico toksikogenomske metode, koja može pružiti molekularni uvid u komplikacije bolesti COVID-19 uslijed izloženosti arsenu, kadmiju,
olovu, živi, niklu i kromu. U tu su svrhu korišteni Komparativna toksikogenomska baza podataka (CTD), GeneMANIA i ToppGene Suite
portal te je identificirana skupina od pet zajedničkih gena (IL1B, CXCL8, IL6, IL10, TNF) za šest metala i COVID-19, koji svi kodiraju
proinflamatorne i antiinflamatorne citokine. Lista je proširena s dodatnih 20 srodnih gena. Fizičke interakcije dominirale su između gena
na koje utječe kombinacija ispitivanih metala (77,64 %), a koekspresija je dominantna interakcija između gena na koje djeluju pojedinačni
metali (As 56,35 %, Cd 64,07 %, Pb 71,5 %, Hg 81,91 %, Ni 64,28 %, Cr 88,51 %). Biološki procesi, molekulske funkcije i putovi u kojima
sudjeluje tih 25 gena blisko su povezani s citokinima i citokinskom olujom, koja je uključena u razvoj komplikacija bolesti COVID-19.
Drugim riječima, ovi rezultati potvrđuju da izloženost toksičnim metalima, bilo pojedinačno ili u kombinaciji, može dovesti do razvoja
težih oblika bolesti COVID-19.
PB  - Institute for Medical Research and Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights
T1  - In silico analiza utjecaja toksičnih metala na komplikacije bolesti COVID-19: molekularni uvidi
VL  - 75
IS  - 2
SP  - 102
EP  - 109
DO  - 10.2478/aiht-2024-75-3819
ER  - 

@article{
author = "Živanović, Jovana and Baralić, Katarina and Živančević, Katarina and Božić, Dragica and Marić, Đurđica and Antonijević Miljaković, Evica and Buha Đorđević, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "COVID-19 can cause a range of complications, including cardiovascular, renal, and/or respiratory insufficiencies, yet little is known of
its potential effects in persons exposed to toxic metals. The aim of this study was to answer this question with in silico toxicogenomic
methods that can provide molecular insights into COVID-19 complications owed to exposure to arsenic, cadmium, lead, mercury, nickel,
and chromium. For this purpose we relied on the Comparative Toxicogenomic Database (CTD), GeneMANIA, and ToppGene Suite
portal and identified a set of five common genes (IL1B, CXCL8, IL6, IL10, TNF) for the six metals and COVID-19, all of which code
for pro-inflammatory and anti-inflammatory cytokines. The list was expanded with additional 20 related genes. Physical interactions are
the most common between the genes affected by the six metals (77.64 %), while the dominant interaction between the genes affected by
each metal separately is co-expression (As 56.35 %, Cd 64.07 %, Pb 71.5 %, Hg 81.91 %, Ni 64.28 %, Cr 88.51 %). Biological processes,
molecular functions, and pathways in which these 25 genes participate are closely related to cytokines and cytokine storm implicated in
the development of COVID-19 complications. In other words, our findings confirm that exposure to toxic metals, alone or in combinations,
might escalate COVID-19 severity., COVID-19 može izazvati niz komplikacija, uključujući kardiovaskularnu, bubrežnu i/ili respiratornu insuficijenciju, ali se malo zna o
njegovim potencijalnim učincima u osoba koje su izložene toksičnim metalima. Cilj ovog istraživanja bio je odgovoriti na to pitanje pomoću
in silico toksikogenomske metode, koja može pružiti molekularni uvid u komplikacije bolesti COVID-19 uslijed izloženosti arsenu, kadmiju,
olovu, živi, niklu i kromu. U tu su svrhu korišteni Komparativna toksikogenomska baza podataka (CTD), GeneMANIA i ToppGene Suite
portal te je identificirana skupina od pet zajedničkih gena (IL1B, CXCL8, IL6, IL10, TNF) za šest metala i COVID-19, koji svi kodiraju
proinflamatorne i antiinflamatorne citokine. Lista je proširena s dodatnih 20 srodnih gena. Fizičke interakcije dominirale su između gena
na koje utječe kombinacija ispitivanih metala (77,64 %), a koekspresija je dominantna interakcija između gena na koje djeluju pojedinačni
metali (As 56,35 %, Cd 64,07 %, Pb 71,5 %, Hg 81,91 %, Ni 64,28 %, Cr 88,51 %). Biološki procesi, molekulske funkcije i putovi u kojima
sudjeluje tih 25 gena blisko su povezani s citokinima i citokinskom olujom, koja je uključena u razvoj komplikacija bolesti COVID-19.
Drugim riječima, ovi rezultati potvrđuju da izloženost toksičnim metalima, bilo pojedinačno ili u kombinaciji, može dovesti do razvoja
težih oblika bolesti COVID-19.",
publisher = "Institute for Medical Research and Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights, In silico analiza utjecaja toksičnih metala na komplikacije bolesti COVID-19: molekularni uvidi",
volume = "75",
number = "2",
pages = "102-109",
doi = "10.2478/aiht-2024-75-3819"
}

Živanović, J., Baralić, K., Živančević, K., Božić, D., Marić, Đ., Antonijević Miljaković, E., Buha Đorđević, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Institute for Medical Research and Occupational Health, Zagreb., 75(2), 102-109.
https://doi.org/10.2478/aiht-2024-75-3819

Živanović J, Baralić K, Živančević K, Božić D, Marić Đ, Antonijević Miljaković E, Buha Đorđević A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2024;75(2):102-109.
doi:10.2478/aiht-2024-75-3819 .

Živanović, Jovana, Baralić, Katarina, Živančević, Katarina, Božić, Dragica, Marić, Đurđica, Antonijević Miljaković, Evica, Buha Đorđević, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "In silico analysis of the impact of toxic metals on COVID-19 complications: molecular insights" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 75, no. 2 (2024):102-109,
https://doi.org/10.2478/aiht-2024-75-3819 . .

TY  - JOUR
AU  - Mulat Simachew, Yonas
AU  - Mihajlović, Marija
AU  - Antonić, Tamara
AU  - Miloševski Lomić, Gordana
AU  - Peco Antić, Amira
AU  - Jovanović, Dragana
AU  - Paripović, Dušan
AU  - Stefanović, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5679
AB  - Background: The pathophysiological mechanisms crucial in
the development of nephrotic syndrome (NS) in the pediatric population are still not fully understood. This study
aimed to investigate the relationship between hypertension, oxidative stress, and inflammation in pediatric
patients during the acute phase of the disease.
Methods: The study included 33 children, aged 2 to 9
years, with nephrotic syndrome. Blood samples were collected during the acute phase and remission. Parameters of
oxidative status were determined, including total oxidative
status (TOS), advanced oxidation protein products (AOPP),
prooxidant-antioxidant balance (PAB), sulfhydryl groups (-
SH), paraoxonase 1 (PON1), and total antioxidant status
(TAS) in serum, measured spectrophotometrically. Inflam-
matory parameters such as pentraxin 3 (PTX3), leptin,
programmed cell death ligand 1 (PD-L1), and E-cadherin
were determined using enzyme-linked immunosorbent
assay (ELISA).
Results: Patients with nephrotic syndrome and hypertension had significantly higher levels of advanced oxidation
protein products and total antioxidant status (p=0.029 and
p=0.003, respectively). During the acute phase of the dis-
ease, lower activity of sulfhydryl groups and paraoxonase 1
was observed compared to remission (p<0.001, for both).
AB  - Uvod: Patofiziološki mehanizmi ključni u razvoju nefrotskog sindroma (NS) u pedijatrijskoj populaciji još uvek nisu u potpunosti razjašnjeni. Ova studija ima za cilj proučavanje sinergističkog delovanja oksidativnog stresa i inflamacije u patogenezi NS. Takođe, jedan od ciljeva ove studije je i ispitivanje veze hipertenzije sa stepenom oksidativnog stresa i inflama - cije kod pacijenata u akutnoj fazi bolesti. Metode: U studiju je uključeno 33 dece sa NS uzrasta od 2 do 9 godina. Uzorci krvi su prikupljeni tokom akutne faze i remisije. Od parametara oksidativnog statusa određivani su: totalni oksidativni status (TOS), uznapredovali proizvodi oksidacije proteina (AOPP), balans prooksidans-antioksidans (PAB), sulfhidrilne grupe (-SH), paraoksonaza 1 (PON1) i ukupan antioksidativni status (TAS) u serumu su mereni spektrofometrijski, a od parametara inflamacije su pentraksin 3 (PTX3), leptin, ligand programirane smrti ćelije 1 (PD-L1) i E-kadherin određivani metodom enzimskog imunosorbentnog testa (ELISA). Rezultati: Pacijenti sa NS i hipertenzijom imali su značajno više nivoe AOPP i TOS (p=0.029 i p=0.003, respektivno). U akutnoj fazi bolesti su uočene nižu aktivnost -SH i PON1 u poređenju sa remisijom (p<0.001, za oba). Nivoi PTX 3 su bili viši, dok su nivoi leptina bili niži tokom akutne faze (p<0.001, za oba). PTX 3 je korelirao sa AOPP i TAS u akutnoj fazi, ali ne i u remisiji (rs=0.42, p=0.027 i rs=0.43, p=0.025,respektivno). U akutnooj fazi utvrđena je negativna korelacija između AOPP i leptina, koja je nestala u remisiji (rs=-0.42, p=0.028). Zaključak: Rezultati ove studije ukazuju da hipertenzija utiče na markere oksidativnog stresa, a smanjeni antioksidativni kapacitet može doprineti razvoju NS. PTX3 se pojavljuje kao potencijalni marker aktivnosti bolesti, što ukazuje na dinamičku vezu između inflamacije i oksidativnog stresa. Leptin može igrati ulogu u oksidativnom stresu u NS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd
T2  - Journal of Medical Biochemistry
T1  - Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation
T1  - Pedijatrijski nefrotski sindrom: međusobna interakcija oksidativnog stresa i inflamacije
VL  - 43
IS  - 3
SP  - 424
EP  - 435
DO  - 10.5937/jomb0-46526
ER  - 

@article{
author = "Mulat Simachew, Yonas and Mihajlović, Marija and Antonić, Tamara and Miloševski Lomić, Gordana and Peco Antić, Amira and Jovanović, Dragana and Paripović, Dušan and Stefanović, Aleksandra",
year = "2024",
abstract = "Background: The pathophysiological mechanisms crucial in
the development of nephrotic syndrome (NS) in the pediatric population are still not fully understood. This study
aimed to investigate the relationship between hypertension, oxidative stress, and inflammation in pediatric
patients during the acute phase of the disease.
Methods: The study included 33 children, aged 2 to 9
years, with nephrotic syndrome. Blood samples were collected during the acute phase and remission. Parameters of
oxidative status were determined, including total oxidative
status (TOS), advanced oxidation protein products (AOPP),
prooxidant-antioxidant balance (PAB), sulfhydryl groups (-
SH), paraoxonase 1 (PON1), and total antioxidant status
(TAS) in serum, measured spectrophotometrically. Inflam-
matory parameters such as pentraxin 3 (PTX3), leptin,
programmed cell death ligand 1 (PD-L1), and E-cadherin
were determined using enzyme-linked immunosorbent
assay (ELISA).
Results: Patients with nephrotic syndrome and hypertension had significantly higher levels of advanced oxidation
protein products and total antioxidant status (p=0.029 and
p=0.003, respectively). During the acute phase of the dis-
ease, lower activity of sulfhydryl groups and paraoxonase 1
was observed compared to remission (p<0.001, for both)., Uvod: Patofiziološki mehanizmi ključni u razvoju nefrotskog sindroma (NS) u pedijatrijskoj populaciji još uvek nisu u potpunosti razjašnjeni. Ova studija ima za cilj proučavanje sinergističkog delovanja oksidativnog stresa i inflamacije u patogenezi NS. Takođe, jedan od ciljeva ove studije je i ispitivanje veze hipertenzije sa stepenom oksidativnog stresa i inflama - cije kod pacijenata u akutnoj fazi bolesti. Metode: U studiju je uključeno 33 dece sa NS uzrasta od 2 do 9 godina. Uzorci krvi su prikupljeni tokom akutne faze i remisije. Od parametara oksidativnog statusa određivani su: totalni oksidativni status (TOS), uznapredovali proizvodi oksidacije proteina (AOPP), balans prooksidans-antioksidans (PAB), sulfhidrilne grupe (-SH), paraoksonaza 1 (PON1) i ukupan antioksidativni status (TAS) u serumu su mereni spektrofometrijski, a od parametara inflamacije su pentraksin 3 (PTX3), leptin, ligand programirane smrti ćelije 1 (PD-L1) i E-kadherin određivani metodom enzimskog imunosorbentnog testa (ELISA). Rezultati: Pacijenti sa NS i hipertenzijom imali su značajno više nivoe AOPP i TOS (p=0.029 i p=0.003, respektivno). U akutnoj fazi bolesti su uočene nižu aktivnost -SH i PON1 u poređenju sa remisijom (p<0.001, za oba). Nivoi PTX 3 su bili viši, dok su nivoi leptina bili niži tokom akutne faze (p<0.001, za oba). PTX 3 je korelirao sa AOPP i TAS u akutnoj fazi, ali ne i u remisiji (rs=0.42, p=0.027 i rs=0.43, p=0.025,respektivno). U akutnooj fazi utvrđena je negativna korelacija između AOPP i leptina, koja je nestala u remisiji (rs=-0.42, p=0.028). Zaključak: Rezultati ove studije ukazuju da hipertenzija utiče na markere oksidativnog stresa, a smanjeni antioksidativni kapacitet može doprineti razvoju NS. PTX3 se pojavljuje kao potencijalni marker aktivnosti bolesti, što ukazuje na dinamičku vezu između inflamacije i oksidativnog stresa. Leptin može igrati ulogu u oksidativnom stresu u NS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd",
journal = "Journal of Medical Biochemistry",
title = "Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation, Pedijatrijski nefrotski sindrom: međusobna interakcija oksidativnog stresa i inflamacije",
volume = "43",
number = "3",
pages = "424-435",
doi = "10.5937/jomb0-46526"
}

Mulat Simachew, Y., Mihajlović, M., Antonić, T., Miloševski Lomić, G., Peco Antić, A., Jovanović, D., Paripović, D.,& Stefanović, A.. (2024). Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd., 43(3), 424-435.
https://doi.org/10.5937/jomb0-46526

Mulat Simachew Y, Mihajlović M, Antonić T, Miloševski Lomić G, Peco Antić A, Jovanović D, Paripović D, Stefanović A. Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation. in Journal of Medical Biochemistry. 2024;43(3):424-435.
doi:10.5937/jomb0-46526 .

Mulat Simachew, Yonas, Mihajlović, Marija, Antonić, Tamara, Miloševski Lomić, Gordana, Peco Antić, Amira, Jovanović, Dragana, Paripović, Dušan, Stefanović, Aleksandra, "Pediatric nephrotic syndrome: the interplay of oxidative stress and inflammation" in Journal of Medical Biochemistry, 43, no. 3 (2024):424-435,
https://doi.org/10.5937/jomb0-46526 . .

TY  - JOUR
AU  - Milinčić, Danijel
AU  - Vidović, Bojana
AU  - Gašić, Uroš
AU  - Milenković, Milan
AU  - Kostić, Aleksandar
AU  - Stanojević, Slađana
AU  - Ilić, Tijana
AU  - Pešić, Mirjana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5677
AB  - The aim of this study was to identify and characterise different classes of bioactive compounds from freeze-dried red goji berries (RGB) grown in Serbia, using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC Q-ToF MS). In addition, this study aims to demonstrate the importance of applying the advanced UHPLC Q-ToF MS technique in the identification of various biocompounds. The analysis showed the presence of 28 phenolic compounds, 3 organic acids, and 26 phenylamides. The 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG) was identified by UHPLC Q-ToF MS and quantified by standardised UHPLC-DAD method. Most of the compounds detected were derivatives of caffeic acid and ferulic acid, followed by quercetin derivatives. Among the phenylamides, several glucosylated caffeoyl and/or dihydrocaffeoyl derivatives of spermidine and spermine were characterized, confirming their recent characterization. Some glycosylated/non-glycosylated putrescine derivatives and caffeoyl-dihydrocaffeoyl-feruloyl spermidines were identified in goji berriesfor the first time. Their tentative structures and fragmentations were proposed.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides
VL  - 456
DO  - 10.1016/j.foodchem.2024.140044
ER  - 

@article{
author = "Milinčić, Danijel and Vidović, Bojana and Gašić, Uroš and Milenković, Milan and Kostić, Aleksandar and Stanojević, Slađana and Ilić, Tijana and Pešić, Mirjana",
year = "2024",
abstract = "The aim of this study was to identify and characterise different classes of bioactive compounds from freeze-dried red goji berries (RGB) grown in Serbia, using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC Q-ToF MS). In addition, this study aims to demonstrate the importance of applying the advanced UHPLC Q-ToF MS technique in the identification of various biocompounds. The analysis showed the presence of 28 phenolic compounds, 3 organic acids, and 26 phenylamides. The 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG) was identified by UHPLC Q-ToF MS and quantified by standardised UHPLC-DAD method. Most of the compounds detected were derivatives of caffeic acid and ferulic acid, followed by quercetin derivatives. Among the phenylamides, several glucosylated caffeoyl and/or dihydrocaffeoyl derivatives of spermidine and spermine were characterized, confirming their recent characterization. Some glycosylated/non-glycosylated putrescine derivatives and caffeoyl-dihydrocaffeoyl-feruloyl spermidines were identified in goji berriesfor the first time. Their tentative structures and fragmentations were proposed.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides",
volume = "456",
doi = "10.1016/j.foodchem.2024.140044"
}

Milinčić, D., Vidović, B., Gašić, U., Milenković, M., Kostić, A., Stanojević, S., Ilić, T.,& Pešić, M.. (2024). A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides. in Food Chemistry
Elsevier Ltd., 456.
https://doi.org/10.1016/j.foodchem.2024.140044

Milinčić D, Vidović B, Gašić U, Milenković M, Kostić A, Stanojević S, Ilić T, Pešić M. A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides. in Food Chemistry. 2024;456.
doi:10.1016/j.foodchem.2024.140044 .

Milinčić, Danijel, Vidović, Bojana, Gašić, Uroš, Milenković, Milan, Kostić, Aleksandar, Stanojević, Slađana, Ilić, Tijana, Pešić, Mirjana, "A systematic UHPLC Q-ToF MS approach for the characterization of bioactive compounds from freeze-dried red goji berries (L. barbarum L.) grown in Serbia: Phenolic compounds and phenylamides" in Food Chemistry, 456 (2024),
https://doi.org/10.1016/j.foodchem.2024.140044 . .

TY  - JOUR
AU  - Senćanski, Dejan
AU  - Tadić, Ivana
AU  - Jocić, Dragana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5678
AB  - Background: Emotional intelligence (EI) is a critical set of skills that impacts clinical pharmacists’ well-being and positively influences high-level patient-centred care. Describing pharmacists’ perceptions may support the integration of EI development approaches into their professional development continuum. Aim: The aim of this study was to analyse pharmacists’ perceptions of the characteristics of emotionally intelligent clinical pharmacists, the importance of EI in clinical practice, and educational models and approaches to enhancing EI. Method: A qualitative study with a focus group methodology was conducted with pharmacy practitioners using a semi-structured guide grounded in the EI competency framework and existing qualitative research methodology practices. Purposive sampling was conducted until information and meaning saturation occurred. The focus group recordings were transcribed and independently coded by two researchers. The conventional content analysis of qualitative data was applied with the inductive thematic approach at its core. Results: According to the 17 focus group participants, emotionally intelligent clinical pharmacists are perceived as self-confident communicators who control and manage emotions, work well under pressure, and handle every situation effectively. Emotional self-control, self-awareness, awareness of others, tolerance, understanding, and empathy have emerged as key EI competencies required for challenges in clinical practice. EI lectures with reflections from clinical applications, behaviour modelling, and behaviour-changing methods were perceived to be of particular importance for pharmacist education and development programmes. Conclusion: Postgraduate pharmacy practitioners perceived EI competencies as necessary for their professional success and high-quality patient-centred care. They suggested that EI competencies be a focal point in pharmacy professional development programmes.
PB  - Springer Nature
T2  - International Journal of Clinical Pharmacy
T1  - Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists
DO  - 10.1007/s11096-024-01748-4
ER  - 

@article{
author = "Senćanski, Dejan and Tadić, Ivana and Jocić, Dragana and Marinković, Valentina",
year = "2024",
abstract = "Background: Emotional intelligence (EI) is a critical set of skills that impacts clinical pharmacists’ well-being and positively influences high-level patient-centred care. Describing pharmacists’ perceptions may support the integration of EI development approaches into their professional development continuum. Aim: The aim of this study was to analyse pharmacists’ perceptions of the characteristics of emotionally intelligent clinical pharmacists, the importance of EI in clinical practice, and educational models and approaches to enhancing EI. Method: A qualitative study with a focus group methodology was conducted with pharmacy practitioners using a semi-structured guide grounded in the EI competency framework and existing qualitative research methodology practices. Purposive sampling was conducted until information and meaning saturation occurred. The focus group recordings were transcribed and independently coded by two researchers. The conventional content analysis of qualitative data was applied with the inductive thematic approach at its core. Results: According to the 17 focus group participants, emotionally intelligent clinical pharmacists are perceived as self-confident communicators who control and manage emotions, work well under pressure, and handle every situation effectively. Emotional self-control, self-awareness, awareness of others, tolerance, understanding, and empathy have emerged as key EI competencies required for challenges in clinical practice. EI lectures with reflections from clinical applications, behaviour modelling, and behaviour-changing methods were perceived to be of particular importance for pharmacist education and development programmes. Conclusion: Postgraduate pharmacy practitioners perceived EI competencies as necessary for their professional success and high-quality patient-centred care. They suggested that EI competencies be a focal point in pharmacy professional development programmes.",
publisher = "Springer Nature",
journal = "International Journal of Clinical Pharmacy",
title = "Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists",
doi = "10.1007/s11096-024-01748-4"
}

Senćanski, D., Tadić, I., Jocić, D.,& Marinković, V.. (2024). Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists. in International Journal of Clinical Pharmacy
Springer Nature..
https://doi.org/10.1007/s11096-024-01748-4

Senćanski D, Tadić I, Jocić D, Marinković V. Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists. in International Journal of Clinical Pharmacy. 2024;.
doi:10.1007/s11096-024-01748-4 .

Senćanski, Dejan, Tadić, Ivana, Jocić, Dragana, Marinković, Valentina, "Perceived importance of emotional intelligence for clinical pharmacy practice and suggested improvements: a focus group study of postgraduate pharmacists" in International Journal of Clinical Pharmacy (2024),
https://doi.org/10.1007/s11096-024-01748-4 . .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Demisli, Sortiria
AU  - Savić, Sanela M.
AU  - Savić, Saša R.
AU  - Randjelović, Danijela V.
AU  - Marković, Bojan
AU  - Pantelić, Ivana
AU  - Mitrović, Jelena
AU  - Stanković, Tijana
AU  - Papadimitrou, Vassiliki
AU  - Xenakis, Aristotelis
AU  - Savić, Snežana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5651
AB  - Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties
VL  - 404
DO  - 10.1016/j.molliq.2024.124888
ER  - 

@article{
author = "Đoković, Jelena and Demisli, Sortiria and Savić, Sanela M. and Savić, Saša R. and Randjelović, Danijela V. and Marković, Bojan and Pantelić, Ivana and Mitrović, Jelena and Stanković, Tijana and Papadimitrou, Vassiliki and Xenakis, Aristotelis and Savić, Snežana",
year = "2024",
abstract = "Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties",
volume = "404",
doi = "10.1016/j.molliq.2024.124888"
}

Đoković, J., Demisli, S., Savić, S. M., Savić, S. R., Randjelović, D. V., Marković, B., Pantelić, I., Mitrović, J., Stanković, T., Papadimitrou, V., Xenakis, A.,& Savić, S.. (2024). Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids
Elsevier B.V.., 404.
https://doi.org/10.1016/j.molliq.2024.124888

Đoković J, Demisli S, Savić SM, Savić SR, Randjelović DV, Marković B, Pantelić I, Mitrović J, Stanković T, Papadimitrou V, Xenakis A, Savić S. Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids. 2024;404.
doi:10.1016/j.molliq.2024.124888 .

Đoković, Jelena, Demisli, Sortiria, Savić, Sanela M., Savić, Saša R., Randjelović, Danijela V., Marković, Bojan, Pantelić, Ivana, Mitrović, Jelena, Stanković, Tijana, Papadimitrou, Vassiliki, Xenakis, Aristotelis, Savić, Snežana, "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties" in Journal of Molecular Liquids, 404 (2024),
https://doi.org/10.1016/j.molliq.2024.124888 . .

TY  - CONF
AU  - Vasiljević, Ivana
AU  - Turković, Erna
AU  - Parojčić, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5675
AB  - Selective laser sintering (SLS) represents a 3D printing technology recently introduced as the drug preparation method (1). It was implemented in the fabrication of different dosage forms, including multiparticulate unit (MPU) systems (2–3).
Up to date, maximal drug content incorporated in the SLS printed dosage forms was 92%, in the case of metronidazole-loaded SLS printed tablets (4). However, in the case of MPUs, the drug load was up to 10% (2–3). Due to the difficulties of incorporating high drug loads into 3D printed dosage forms, the aim of this research was to investigate the possibility of printing multiparticulate unit systems containing higher drug loading and evaluate their characteristics (model drug content, particle size and size distribution, flowability, and drug dissolution).
PB  - International Association for Pharmaceutical Technology (APV), Mainz, Germany
PB  - International Society of Drug Delivery Sciences and Technology (APGI), Lille, France
PB  - Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy
C3  - 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
T1  - How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5675
ER  - 

@conference{
author = "Vasiljević, Ivana and Turković, Erna and Parojčić, Jelena",
year = "2024",
abstract = "Selective laser sintering (SLS) represents a 3D printing technology recently introduced as the drug preparation method (1). It was implemented in the fabrication of different dosage forms, including multiparticulate unit (MPU) systems (2–3).
Up to date, maximal drug content incorporated in the SLS printed dosage forms was 92%, in the case of metronidazole-loaded SLS printed tablets (4). However, in the case of MPUs, the drug load was up to 10% (2–3). Due to the difficulties of incorporating high drug loads into 3D printed dosage forms, the aim of this research was to investigate the possibility of printing multiparticulate unit systems containing higher drug loading and evaluate their characteristics (model drug content, particle size and size distribution, flowability, and drug dissolution).",
publisher = "International Association for Pharmaceutical Technology (APV), Mainz, Germany, International Society of Drug Delivery Sciences and Technology (APGI), Lille, France, Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy",
journal = "14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria",
title = "How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5675"
}

Vasiljević, I., Turković, E.,& Parojčić, J.. (2024). How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
International Association for Pharmaceutical Technology (APV), Mainz, Germany..
https://hdl.handle.net/21.15107/rcub_farfar_5675

Vasiljević I, Turković E, Parojčić J. How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria. 2024;.
https://hdl.handle.net/21.15107/rcub_farfar_5675 .

Vasiljević, Ivana, Turković, Erna, Parojčić, Jelena, "How does the ibuprofen content affect the characteristics of multiparticulate unit systems prepared by selective laser sintering?" in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria (2024),
https://hdl.handle.net/21.15107/rcub_farfar_5675 .

TY  - CONF
AU  - Stanković, Milan
AU  - Đurđević, Mladen
AU  - Ražić, Slavica
AU  - Đogo-Mračević, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5673
AB  - Za zemljišta urbanih gradskih područja se očekuje da budu zagađena potencijalno toksičnim elementima (PTE), zbog saobraćaja, urbanizacije i industralizacij. Budući da deca provode dosta vremena na igralištima, igrajući se zemljom, njihova izloženost ovim elementima i potencijalni štetni efekti na zdravlje su poslednjih godina postala vrlo značajna tema. [1-2] Cilj ovog rada je bio određivanje sadržaja arsena (As), kadmijuma (Cd), olova (Pb), nikla (Ni), hroma (Cr), bakra (Cu) i cinka (Zn) u 17 uzoraka zemljišta sa različitih dečjih igrališta u urbanoj zoni Beograda. Uzorci su pripremani kiselom mikrotalasnom digestijom, a sadržaj elemenata je određivan metodom induktivno spregnute plazme optičke emisione spektroskopije (ISP-OES). Tačnost metode je procenjena upotrebom sertifikovanog referentnog materijala NIST 2711a, i dobijene su vrednosti  u opsegu  80-120%. Prosečna koncentracija ispitivanih PTE sledi navedeni niz: Zn (68,24 mg/kg) > Ni (60,99 mg/kg) > Cr (51,685 mg/kg)  > Pb (21,615 mg/kg) > Cu (19,785 mg/kg) > As (8,1125 mg/kg) > Cd (0,1566 mg/kg). Koncentracije svih ispitivanih PTE su u saglasnosti sa  legislativma Republike Srbije, Evropske unije (EU) i Svetske zdravstvene organizacije (SZO). S obzirom na potencijal PTE za bioakumulaciju i uključivanje u lanac ishran, vrlo je važno njihovo konstantno praćenje, a u tom smislu ovaj rad može imati doprinos u očuvanju urbane sredine.
AB  - Soils in the urban area are suspected to be contaminated with potentially toxic elements (PTEs), due to traffic, urbanisation, and industrialization. The possibility of adverse health effects and the exposure of children who spend a lot of time in playgrounds and playing with soil, has attracted  a lot of attention in recent years, especially with regard to the potential risk to children's health. [1-2] The aim of this study was to determine the levels of arsenic (As), cadmium (Cd), lead (Pb), nickel (Ni), chromium (Cr), copper (Cu), and zinc (Zn) in 17 soil samples from different children's playgrounds in the city of Belgrade. The samples were prepared by acid assisted microwave digestion and the concentrations of  elements were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Accuracy was assessed using the certified reference material NIST 2711a, and recoveries obtained were in the range of 80-120%. The average concentration of PTEs followed the following order: Zn (68.24 mg/kg) > Ni (60.99 mg/kg) > Cr (51.685 mg/kg)  > Pb (21.615 mg/kg) > Cu (19.785 mg/kg) > As (8.1125 mg/kg) > Cd (0.1566 mg/kg). The average concentration of all metals in soil did not exceed the permissible limits according to the guidelines of Serbian legislation, the European Union (EU), and the World Health Organization (WHO).  Since these PTEs can bioaccumulate and biomagnify in the food chain, their concentration in soil should be continuously monitored so this study should contribute to rising awareness for the protection of the urban environment.

[1] M. Guney, G. J. Zagury, N. Dogan, T. T. Onay. Exposure assessment and risk characterization from trace elements following soil ingestion by children exposed to playgrounds, parks and picnic areas, J Hazard Mater 2010;182(1-3):656-64. 
[2] E. Schachter, A. Gailey, S. Perl Egendorf, H. W. Mielke, Mechanisms of children's soil exposure, Current Problems in Pediatric and Adolescent Health Care 2020, 50(1), 100742.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija
T1  - Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda
T1  - Toxic Elements in Soil of Children's Playgrounds in the Belgrade Urban Area
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5673
ER  - 

@conference{
author = "Stanković, Milan and Đurđević, Mladen and Ražić, Slavica and Đogo-Mračević, Svetlana",
year = "2024",
abstract = "Za zemljišta urbanih gradskih područja se očekuje da budu zagađena potencijalno toksičnim elementima (PTE), zbog saobraćaja, urbanizacije i industralizacij. Budući da deca provode dosta vremena na igralištima, igrajući se zemljom, njihova izloženost ovim elementima i potencijalni štetni efekti na zdravlje su poslednjih godina postala vrlo značajna tema. [1-2] Cilj ovog rada je bio određivanje sadržaja arsena (As), kadmijuma (Cd), olova (Pb), nikla (Ni), hroma (Cr), bakra (Cu) i cinka (Zn) u 17 uzoraka zemljišta sa različitih dečjih igrališta u urbanoj zoni Beograda. Uzorci su pripremani kiselom mikrotalasnom digestijom, a sadržaj elemenata je određivan metodom induktivno spregnute plazme optičke emisione spektroskopije (ISP-OES). Tačnost metode je procenjena upotrebom sertifikovanog referentnog materijala NIST 2711a, i dobijene su vrednosti  u opsegu  80-120%. Prosečna koncentracija ispitivanih PTE sledi navedeni niz: Zn (68,24 mg/kg) > Ni (60,99 mg/kg) > Cr (51,685 mg/kg)  > Pb (21,615 mg/kg) > Cu (19,785 mg/kg) > As (8,1125 mg/kg) > Cd (0,1566 mg/kg). Koncentracije svih ispitivanih PTE su u saglasnosti sa  legislativma Republike Srbije, Evropske unije (EU) i Svetske zdravstvene organizacije (SZO). S obzirom na potencijal PTE za bioakumulaciju i uključivanje u lanac ishran, vrlo je važno njihovo konstantno praćenje, a u tom smislu ovaj rad može imati doprinos u očuvanju urbane sredine., Soils in the urban area are suspected to be contaminated with potentially toxic elements (PTEs), due to traffic, urbanisation, and industrialization. The possibility of adverse health effects and the exposure of children who spend a lot of time in playgrounds and playing with soil, has attracted  a lot of attention in recent years, especially with regard to the potential risk to children's health. [1-2] The aim of this study was to determine the levels of arsenic (As), cadmium (Cd), lead (Pb), nickel (Ni), chromium (Cr), copper (Cu), and zinc (Zn) in 17 soil samples from different children's playgrounds in the city of Belgrade. The samples were prepared by acid assisted microwave digestion and the concentrations of  elements were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Accuracy was assessed using the certified reference material NIST 2711a, and recoveries obtained were in the range of 80-120%. The average concentration of PTEs followed the following order: Zn (68.24 mg/kg) > Ni (60.99 mg/kg) > Cr (51.685 mg/kg)  > Pb (21.615 mg/kg) > Cu (19.785 mg/kg) > As (8.1125 mg/kg) > Cd (0.1566 mg/kg). The average concentration of all metals in soil did not exceed the permissible limits according to the guidelines of Serbian legislation, the European Union (EU), and the World Health Organization (WHO).  Since these PTEs can bioaccumulate and biomagnify in the food chain, their concentration in soil should be continuously monitored so this study should contribute to rising awareness for the protection of the urban environment.

[1] M. Guney, G. J. Zagury, N. Dogan, T. T. Onay. Exposure assessment and risk characterization from trace elements following soil ingestion by children exposed to playgrounds, parks and picnic areas, J Hazard Mater 2010;182(1-3):656-64. 
[2] E. Schachter, A. Gailey, S. Perl Egendorf, H. W. Mielke, Mechanisms of children's soil exposure, Current Problems in Pediatric and Adolescent Health Care 2020, 50(1), 100742.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija",
title = "Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda, Toxic Elements in Soil of Children's Playgrounds in the Belgrade Urban Area",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5673"
}

Stanković, M., Đurđević, M., Ražić, S.,& Đogo-Mračević, S.. (2024). Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda. in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija
Beograd : Srpsko hemijsko društvo., 81-81.
https://hdl.handle.net/21.15107/rcub_farfar_5673

Stanković M, Đurđević M, Ražić S, Đogo-Mračević S. Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda. in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija. 2024;:81-81.
https://hdl.handle.net/21.15107/rcub_farfar_5673 .

Stanković, Milan, Đurđević, Mladen, Ražić, Slavica, Đogo-Mračević, Svetlana, "Sadržaj toksičnih elemenata u zemljištu dečjih igrališta urbanog područja Beograda" in 60. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 8 i 9. jun 2024, Niš, Srbija (2024):81-81,
https://hdl.handle.net/21.15107/rcub_farfar_5673 .

TY  - JOUR
AU  - Kovačević, Peđa
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Barišić, Vedrana
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
AU  - Rizwan, Zeeshan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5631
AB  - Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Naunyn-Schmiedeberg's Archives of Pharmacology
T1  - Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study
DO  - 10.1007/s00210-024-03136-1
ER  - 

@article{
author = "Kovačević, Peđa and Milaković, Dragana and Kovačević, Tijana and Barišić, Vedrana and Dragić, Saša and Zlojutro, Biljana and Miljković, Branislava and Vučićević, Katarina and Rizwan, Zeeshan",
year = "2024",
abstract = "Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Naunyn-Schmiedeberg's Archives of Pharmacology",
title = "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study",
doi = "10.1007/s00210-024-03136-1"
}

Kovačević, P., Milaković, D., Kovačević, T., Barišić, V., Dragić, S., Zlojutro, B., Miljković, B., Vučićević, K.,& Rizwan, Z.. (2024). Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00210-024-03136-1

Kovačević P, Milaković D, Kovačević T, Barišić V, Dragić S, Zlojutro B, Miljković B, Vučićević K, Rizwan Z. Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology. 2024;.
doi:10.1007/s00210-024-03136-1 .

Kovačević, Peđa, Milaković, Dragana, Kovačević, Tijana, Barišić, Vedrana, Dragić, Saša, Zlojutro, Biljana, Miljković, Branislava, Vučićević, Katarina, Rizwan, Zeeshan, "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study" in Naunyn-Schmiedeberg's Archives of Pharmacology (2024),
https://doi.org/10.1007/s00210-024-03136-1 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
year = "2024",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I.. (2024). Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines. 2024;12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4 (2024),
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Adamov, Ivana
AU  - Stanojević, Gordana
AU  - Pavlović, Stefan
AU  - Medarević, Đorđe
AU  - Ivković, Branka
AU  - Kočović, David
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5630
AB  - Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets
VL  - 657
DO  - 10.1016/j.ijpharm.2024.124161
ER  - 

@article{
author = "Adamov, Ivana and Stanojević, Gordana and Pavlović, Stefan and Medarević, Đorđe and Ivković, Branka and Kočović, David and Ibrić, Svetlana",
year = "2024",
abstract = "Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets",
volume = "657",
doi = "10.1016/j.ijpharm.2024.124161"
}

Adamov, I., Stanojević, G., Pavlović, S., Medarević, Đ., Ivković, B., Kočović, D.,& Ibrić, S.. (2024). Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics
Elsevier B.V.., 657.
https://doi.org/10.1016/j.ijpharm.2024.124161

Adamov I, Stanojević G, Pavlović S, Medarević Đ, Ivković B, Kočović D, Ibrić S. Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics. 2024;657.
doi:10.1016/j.ijpharm.2024.124161 .

Adamov, Ivana, Stanojević, Gordana, Pavlović, Stefan, Medarević, Đorđe, Ivković, Branka, Kočović, David, Ibrić, Svetlana, "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets" in International Journal of Pharmaceutics, 657 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124161 . .

TY  - JOUR
AU  - Jovanović, Aleksandar
AU  - Drobac, Milica
AU  - Vidović, Bojana
AU  - Pavlović, Dragana
AU  - Krajnović, Dušanka
AU  - Tadić, Ivana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5658
AB  - ntroduction: Urinary tract infections (UTIs) are one of the most common bacterial infections accompanied by inflammation in any part of the urinary system. Antibiotics are the gold standard for UTI treatment; however, herbal products are also administered. The study aimed to assess patients' attitudes and opinions regarding the use and effectiveness of antibiotics and herbal products for the treatment of UTIs. Methods: A quantitative, non-experimental study was conducted using an online survey in the Republic of Serbia in 2021 and 2022. Results: The study comprised 393 respondents, with average age of 34.66 years and most respondents being women (91.9%). According to the personal assessment, slightly less than half of the respondents (46.1%) classified UTI symptoms as severe. Respondents predominantly treated UTI symptoms with herbal products combined with antibiotics (52.2%) or herbal products alone (26.6%). The most frequently used antibiotics were ciprofloxacin (20.9%), cephalexin (16.9%), and sulfamethoxazole/trimethoprim (15.4%). As many as 74.3% of respondents used herbal teas. The most used herbal products contained bearberry leaf (Uvae ursi folium) (52.1%), parsley leaf/root (Petroselini foium/radix) (15.1%), and cranberry fruit (Vaccinii macrocarpi fructus) (12.6%). Self-assessment of the effectiveness of UTI treatment with antibiotics and herbal products was high. Conclusion: The antibiotics used to treat UTIs did not align with the guidelines of the European Association of Urologists. Further, the use of herbal products was frequently observed. Therefore, community pharmacists, who serve as the most accessible healthcare professionals with competent knowledge about herbal products, should adequately inform patients about potential therapeutic options for UTI.
PB  - Elsevier B.V.
T2  - Journal of Herbal Medicine
T1  - Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes
VL  - 46
DO  - 10.1016/j.hermed.2024.100892
ER  - 

@article{
author = "Jovanović, Aleksandar and Drobac, Milica and Vidović, Bojana and Pavlović, Dragana and Krajnović, Dušanka and Tadić, Ivana",
year = "2024",
abstract = "ntroduction: Urinary tract infections (UTIs) are one of the most common bacterial infections accompanied by inflammation in any part of the urinary system. Antibiotics are the gold standard for UTI treatment; however, herbal products are also administered. The study aimed to assess patients' attitudes and opinions regarding the use and effectiveness of antibiotics and herbal products for the treatment of UTIs. Methods: A quantitative, non-experimental study was conducted using an online survey in the Republic of Serbia in 2021 and 2022. Results: The study comprised 393 respondents, with average age of 34.66 years and most respondents being women (91.9%). According to the personal assessment, slightly less than half of the respondents (46.1%) classified UTI symptoms as severe. Respondents predominantly treated UTI symptoms with herbal products combined with antibiotics (52.2%) or herbal products alone (26.6%). The most frequently used antibiotics were ciprofloxacin (20.9%), cephalexin (16.9%), and sulfamethoxazole/trimethoprim (15.4%). As many as 74.3% of respondents used herbal teas. The most used herbal products contained bearberry leaf (Uvae ursi folium) (52.1%), parsley leaf/root (Petroselini foium/radix) (15.1%), and cranberry fruit (Vaccinii macrocarpi fructus) (12.6%). Self-assessment of the effectiveness of UTI treatment with antibiotics and herbal products was high. Conclusion: The antibiotics used to treat UTIs did not align with the guidelines of the European Association of Urologists. Further, the use of herbal products was frequently observed. Therefore, community pharmacists, who serve as the most accessible healthcare professionals with competent knowledge about herbal products, should adequately inform patients about potential therapeutic options for UTI.",
publisher = "Elsevier B.V.",
journal = "Journal of Herbal Medicine",
title = "Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes",
volume = "46",
doi = "10.1016/j.hermed.2024.100892"
}

Jovanović, A., Drobac, M., Vidović, B., Pavlović, D., Krajnović, D.,& Tadić, I.. (2024). Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes. in Journal of Herbal Medicine
Elsevier B.V.., 46.
https://doi.org/10.1016/j.hermed.2024.100892

Jovanović A, Drobac M, Vidović B, Pavlović D, Krajnović D, Tadić I. Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes. in Journal of Herbal Medicine. 2024;46.
doi:10.1016/j.hermed.2024.100892 .

Jovanović, Aleksandar, Drobac, Milica, Vidović, Bojana, Pavlović, Dragana, Krajnović, Dušanka, Tadić, Ivana, "Herbal products versus antibiotics for urinary tract infections-analysis of patient attitudes" in Journal of Herbal Medicine, 46 (2024),
https://doi.org/10.1016/j.hermed.2024.100892 . .

TY  - JOUR
AU  - Ćurčić, Vladimir
AU  - Olszewski, Mateusz
AU  - Maciejewska, Natalia
AU  - Višnjevac, Aleksandar
AU  - Srdić-Rajić, Tatjana
AU  - Dobričić, Vladimir
AU  - García-Sosa, Alfonso T.
AU  - Kokanov, Sanja B.
AU  - Araškov, Jovana B.
AU  - Silvestri, Romano
AU  - Schüle, Roland
AU  - Jung, Manfred
AU  - Nikolić, Milan
AU  - Filipović, Nenad R.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5298
AB  - Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.
PB  - John Wiley and Sons Inc
T2  - Archiv der Pharmazie
T1  - Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition
VL  - 357
IS  - 2
SP  - 2300426
DO  - 10.1002/ardp.202300426
ER  - 

@article{
author = "Ćurčić, Vladimir and Olszewski, Mateusz and Maciejewska, Natalia and Višnjevac, Aleksandar and Srdić-Rajić, Tatjana and Dobričić, Vladimir and García-Sosa, Alfonso T. and Kokanov, Sanja B. and Araškov, Jovana B. and Silvestri, Romano and Schüle, Roland and Jung, Manfred and Nikolić, Milan and Filipović, Nenad R.",
year = "2024",
abstract = "Heterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based on the 8-quinoline (1a–c), 2-quinoline (2a–c), and 8-hydroxy-2-quinolyl moiety (3a–c). The panel of several human cancer cell lines and the nontumorigenic human embryonic kidney cell line HEK-293 were used to evaluate the compound-mediated in vitro anticancer activities, leading to [2-(2-(quinolyl-8-ol-2-ylmethylene)hydrazinyl)]-4-(4-methoxyphenyl)-1,3-thiazole (3c) as the most promising compound. The study revealed that 3c blocks the cell-cycle progression of a human colon cancer cell line (HCT-116) in the S phase and induces DNA double-strand breaks. Also, our findings demonstrate that 3c accumulates in lysosomes, ultimately leading to the cell death of the hepatocellular carcinoma cell line (Hep-G2) and HCT-116 cells, by the mechanism of autophagy inhibition.",
publisher = "John Wiley and Sons Inc",
journal = "Archiv der Pharmazie",
title = "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition",
volume = "357",
number = "2",
pages = "2300426",
doi = "10.1002/ardp.202300426"
}

Ćurčić, V., Olszewski, M., Maciejewska, N., Višnjevac, A., Srdić-Rajić, T., Dobričić, V., García-Sosa, A. T., Kokanov, S. B., Araškov, J. B., Silvestri, R., Schüle, R., Jung, M., Nikolić, M.,& Filipović, N. R.. (2024). Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie
John Wiley and Sons Inc., 357(2), 2300426.
https://doi.org/10.1002/ardp.202300426

Ćurčić V, Olszewski M, Maciejewska N, Višnjevac A, Srdić-Rajić T, Dobričić V, García-Sosa AT, Kokanov SB, Araškov JB, Silvestri R, Schüle R, Jung M, Nikolić M, Filipović NR. Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition. in Archiv der Pharmazie. 2024;357(2):2300426.
doi:10.1002/ardp.202300426 .

Ćurčić, Vladimir, Olszewski, Mateusz, Maciejewska, Natalia, Višnjevac, Aleksandar, Srdić-Rajić, Tatjana, Dobričić, Vladimir, García-Sosa, Alfonso T., Kokanov, Sanja B., Araškov, Jovana B., Silvestri, Romano, Schüle, Roland, Jung, Manfred, Nikolić, Milan, Filipović, Nenad R., "Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition" in Archiv der Pharmazie, 357, no. 2 (2024):2300426,
https://doi.org/10.1002/ardp.202300426 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Milinković Budinčić, Jelena
AU  - Stanić, Dušanka
AU  - Fraj, Jadranka
AU  - Petrović, Lidija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5647
AB  - The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.
PB  - MDPI
T2  - Pharmaceutics
T1  - Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050628
ER  - 

@article{
author = "Đekić, Ljiljana and Milinković Budinčić, Jelena and Stanić, Dušanka and Fraj, Jadranka and Petrović, Lidija",
year = "2024",
abstract = "The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050628"
}

Đekić, L., Milinković Budinčić, J., Stanić, D., Fraj, J.,& Petrović, L.. (2024). Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050628

Đekić L, Milinković Budinčić J, Stanić D, Fraj J, Petrović L. Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050628 .

Đekić, Ljiljana, Milinković Budinčić, Jelena, Stanić, Dušanka, Fraj, Jadranka, Petrović, Lidija, "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050628 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Stefanović, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5622
AB  - Childhood obesity with its growing prevalence worldwide presents one of the most important health challenges nowadays. Multiple mechanisms are involved in the development of this condition, as well as in its associations with various cardiometabolic complications, such as insulin resistance, diabetes, metabolic dysfunction-associated steatotic liver disease and cardiovascular diseases. Recent findings suggest that childhood obesity and associated dyslipidemia at least partly originate from epigenetic modifications that take place in the earliest periods of life, namely prenatal and perinatal periods. Hence, alterations of maternal metabolism could be fundamentally responsible for fetal and neonatal metabolic programming and consequently, for metabolic health of offspring in later life. In this paper, we will review recent findings on the associations among intrauterine and early postnatal exposure to undesirable modulators of metabolism, development of childhood obesity and later cardiometabolic complications. Special attention will be given to maternal dyslipidemia as a driven force for undesirable epigenetic modulations in offspring. In addition, newly proposed lipid biomarkers of increased cardiometabolic risk in obese children and adolescents will be analyzed, with respect to their predictive potential and clinical applicability.
PB  - W.B. Saunders
T2  - Metabolism: Clinical and Experimental
T1  - Obesity and dyslipidemia in early life: Impact on cardiometabolic risk
VL  - 156
DO  - 10.1016/j.metabol.2024.155919
ER  - 

@article{
author = "Zeljković, Aleksandra and Vekić, Jelena and Stefanović, Aleksandra",
year = "2024",
abstract = "Childhood obesity with its growing prevalence worldwide presents one of the most important health challenges nowadays. Multiple mechanisms are involved in the development of this condition, as well as in its associations with various cardiometabolic complications, such as insulin resistance, diabetes, metabolic dysfunction-associated steatotic liver disease and cardiovascular diseases. Recent findings suggest that childhood obesity and associated dyslipidemia at least partly originate from epigenetic modifications that take place in the earliest periods of life, namely prenatal and perinatal periods. Hence, alterations of maternal metabolism could be fundamentally responsible for fetal and neonatal metabolic programming and consequently, for metabolic health of offspring in later life. In this paper, we will review recent findings on the associations among intrauterine and early postnatal exposure to undesirable modulators of metabolism, development of childhood obesity and later cardiometabolic complications. Special attention will be given to maternal dyslipidemia as a driven force for undesirable epigenetic modulations in offspring. In addition, newly proposed lipid biomarkers of increased cardiometabolic risk in obese children and adolescents will be analyzed, with respect to their predictive potential and clinical applicability.",
publisher = "W.B. Saunders",
journal = "Metabolism: Clinical and Experimental",
title = "Obesity and dyslipidemia in early life: Impact on cardiometabolic risk",
volume = "156",
doi = "10.1016/j.metabol.2024.155919"
}

Zeljković, A., Vekić, J.,& Stefanović, A.. (2024). Obesity and dyslipidemia in early life: Impact on cardiometabolic risk. in Metabolism: Clinical and Experimental
W.B. Saunders., 156.
https://doi.org/10.1016/j.metabol.2024.155919

Zeljković A, Vekić J, Stefanović A. Obesity and dyslipidemia in early life: Impact on cardiometabolic risk. in Metabolism: Clinical and Experimental. 2024;156.
doi:10.1016/j.metabol.2024.155919 .

Zeljković, Aleksandra, Vekić, Jelena, Stefanović, Aleksandra, "Obesity and dyslipidemia in early life: Impact on cardiometabolic risk" in Metabolism: Clinical and Experimental, 156 (2024),
https://doi.org/10.1016/j.metabol.2024.155919 . .

TY  - JOUR
AU  - Pejčić, Zorica
AU  - Topić Vučenović, Valentina
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5649
AB  - Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.
PB  - MDPI
T2  - Pharmaceutics
T1  - Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050685
ER  - 

@article{
author = "Pejčić, Zorica and Topić Vučenović, Valentina and Miljković, Branislava and Vučićević, Katarina",
year = "2024",
abstract = "Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050685"
}

Pejčić, Z., Topić Vučenović, V., Miljković, B.,& Vučićević, K.. (2024). Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050685

Pejčić Z, Topić Vučenović V, Miljković B, Vučićević K. Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050685 .

Pejčić, Zorica, Topić Vučenović, Valentina, Miljković, Branislava, Vučićević, Katarina, "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050685 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Munjas, Jelena
AU  - Kovačević, Smiljka
AU  - Guzonjić, Azra
AU  - Mitrović, Jadranka
AU  - Saso, Luciano
AU  - Đuričić, Ivana
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5654
AB  - Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Childhood obesity accelerates biological ageing: is oxidative stress a link?
DO  - 10.1017/S0007114524000898
ER  - 

@article{
author = "Subošić, Branko and Zdravković, Vera and Ješić, Maja and Munjas, Jelena and Kovačević, Smiljka and Guzonjić, Azra and Mitrović, Jadranka and Saso, Luciano and Đuričić, Ivana and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Childhood obesity accelerates biological ageing: is oxidative stress a link?",
doi = "10.1017/S0007114524000898"
}

Subošić, B., Zdravković, V., Ješić, M., Munjas, J., Kovačević, S., Guzonjić, A., Mitrović, J., Saso, L., Đuričić, I.,& Kotur-Stevuljević, J.. (2024). Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition
Cambridge University Press..
https://doi.org/10.1017/S0007114524000898

Subošić B, Zdravković V, Ješić M, Munjas J, Kovačević S, Guzonjić A, Mitrović J, Saso L, Đuričić I, Kotur-Stevuljević J. Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition. 2024;.
doi:10.1017/S0007114524000898 .

Subošić, Branko, Zdravković, Vera, Ješić, Maja, Munjas, Jelena, Kovačević, Smiljka, Guzonjić, Azra, Mitrović, Jadranka, Saso, Luciano, Đuričić, Ivana, Kotur-Stevuljević, Jelena, "Childhood obesity accelerates biological ageing: is oxidative stress a link?" in British Journal of Nutrition (2024),
https://doi.org/10.1017/S0007114524000898 . .

TY  - JOUR
AU  - Turković, Erna
AU  - Vasiljević, Ivana
AU  - Parojčić, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5650
AB  - Orodispersible films (ODFs) have emerged as innovative pharmaceutical dosage forms, offering patient-specific treatment through adjustable dosing and the combination of diverse active ingredients. This expanding field generates vast datasets, requiring advanced analytical techniques for deeper understanding of data itself. Machine learning is becoming an important tool in the rapidly changing field of pharmaceutical research, particularly in drug preformulation studies. This work aims to explore into the application of machine learning methods for the analysis of experimental data obtained by ODF characterization in order to obtain an insight into the factors governing ODF performance and use it as guidance in pharmaceutical development. Using a dataset derived from extensive experimental studies, various machine learning algorithms were employed to cluster and predict critical properties of ODFs. Our results demonstrate that machine learning models, including Support vector machine, Random forest and Deep learning, exhibit high accuracy in predicting the mechanical properties of ODFs, such as flexibility and rigidity. The predictive models offered insights into the complex interaction of formulation variables. This research is a pilot study that highlights the potential of machine learning as a transformative approach in the pharmaceutical field, paving the way for more efficient and informed drug development processes.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development
VL  - 658
DO  - 10.1016/j.ijpharm.2024.124188
ER  - 

@article{
author = "Turković, Erna and Vasiljević, Ivana and Parojčić, Jelena",
year = "2024",
abstract = "Orodispersible films (ODFs) have emerged as innovative pharmaceutical dosage forms, offering patient-specific treatment through adjustable dosing and the combination of diverse active ingredients. This expanding field generates vast datasets, requiring advanced analytical techniques for deeper understanding of data itself. Machine learning is becoming an important tool in the rapidly changing field of pharmaceutical research, particularly in drug preformulation studies. This work aims to explore into the application of machine learning methods for the analysis of experimental data obtained by ODF characterization in order to obtain an insight into the factors governing ODF performance and use it as guidance in pharmaceutical development. Using a dataset derived from extensive experimental studies, various machine learning algorithms were employed to cluster and predict critical properties of ODFs. Our results demonstrate that machine learning models, including Support vector machine, Random forest and Deep learning, exhibit high accuracy in predicting the mechanical properties of ODFs, such as flexibility and rigidity. The predictive models offered insights into the complex interaction of formulation variables. This research is a pilot study that highlights the potential of machine learning as a transformative approach in the pharmaceutical field, paving the way for more efficient and informed drug development processes.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development",
volume = "658",
doi = "10.1016/j.ijpharm.2024.124188"
}

Turković, E., Vasiljević, I.,& Parojčić, J.. (2024). A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development. in International Journal of Pharmaceutics
Elsevier B.V.., 658.
https://doi.org/10.1016/j.ijpharm.2024.124188

Turković E, Vasiljević I, Parojčić J. A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development. in International Journal of Pharmaceutics. 2024;658.
doi:10.1016/j.ijpharm.2024.124188 .

Turković, Erna, Vasiljević, Ivana, Parojčić, Jelena, "A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development" in International Journal of Pharmaceutics, 658 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124188 . .

TY  - JOUR
AU  - Bećirović, Sabina
AU  - Vuković, Bojana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5632
AB  - The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.
PB  - Taylor and Francis Ltd.
T2  - Biotechnology and Biotechnological Equipment
T1  - Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina
VL  - 38
IS  - 1
DO  - 10.1080/13102818.2024.2349593
ER  - 

@article{
author = "Bećirović, Sabina and Vuković, Bojana and Marinković, Valentina",
year = "2024",
abstract = "The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.",
publisher = "Taylor and Francis Ltd.",
journal = "Biotechnology and Biotechnological Equipment",
title = "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina",
volume = "38",
number = "1",
doi = "10.1080/13102818.2024.2349593"
}

Bećirović, S., Vuković, B.,& Marinković, V.. (2024). Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment
Taylor and Francis Ltd.., 38(1).
https://doi.org/10.1080/13102818.2024.2349593

Bećirović S, Vuković B, Marinković V. Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment. 2024;38(1).
doi:10.1080/13102818.2024.2349593 .

Bećirović, Sabina, Vuković, Bojana, Marinković, Valentina, "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina" in Biotechnology and Biotechnological Equipment, 38, no. 1 (2024),
https://doi.org/10.1080/13102818.2024.2349593 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljević, Milica
AU  - Janković, Radmila
AU  - Đuretić, Jasmina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5626
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
PB  - MDPI
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
VL  - 12
IS  - 4
SP  - 717
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Biljana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljević, Milica and Janković, Radmila and Đuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.",
publisher = "MDPI",
journal = "Biomedicines",
title = "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats",
volume = "12",
number = "4",
pages = "717",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljević, M., Janković, R.,& Đuretić, J.. (2024). NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
MDPI., 12(4), 717.
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljević M, Janković R, Đuretić J. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4):717.
doi:10.3390/biomedicines12040717 .

Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024):717,
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Radan, Milica
AU  - Milivojević, Dušan
AU  - Stević, Tatjana
AU  - Pljevljakušić, Dejan
AU  - Nikodinović-Runić, Jasmina
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5642
AB  - Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii
VL  - 216
DO  - 10.1016/j.indcrop.2024.118762
ER  - 

@article{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Radan, Milica and Milivojević, Dušan and Stević, Tatjana and Pljevljakušić, Dejan and Nikodinović-Runić, Jasmina and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii",
volume = "216",
doi = "10.1016/j.indcrop.2024.118762"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Radan, M., Milivojević, D., Stević, T., Pljevljakušić, D., Nikodinović-Runić, J., Bigović, D., Šavikin, K.,& Filipić, B.. (2024). Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products
Elsevier B.V.., 216.
https://doi.org/10.1016/j.indcrop.2024.118762

Kuzmanović Nedeljković S, Ćujić Nikolić N, Radan M, Milivojević D, Stević T, Pljevljakušić D, Nikodinović-Runić J, Bigović D, Šavikin K, Filipić B. Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products. 2024;216.
doi:10.1016/j.indcrop.2024.118762 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Radan, Milica, Milivojević, Dušan, Stević, Tatjana, Pljevljakušić, Dejan, Nikodinović-Runić, Jasmina, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii" in Industrial Crops and Products, 216 (2024),
https://doi.org/10.1016/j.indcrop.2024.118762 . .

TY  - JOUR
AU  - Andrys, Rudolf
AU  - Monnier, Charline
AU  - Antonijević-Miljaković, Evica
AU  - Mickova, Veronika
AU  - Musilek, Kamil
AU  - Zemanova, Lucie
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5643
AB  - Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.
PB  - Elsevier B.V.
T2  - Talanta
T1  - Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research
VL  - 276
DO  - 10.1016/j.talanta.2024.126263
ER  - 

@article{
author = "Andrys, Rudolf and Monnier, Charline and Antonijević-Miljaković, Evica and Mickova, Veronika and Musilek, Kamil and Zemanova, Lucie",
year = "2024",
abstract = "Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.",
publisher = "Elsevier B.V.",
journal = "Talanta",
title = "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research",
volume = "276",
doi = "10.1016/j.talanta.2024.126263"
}

Andrys, R., Monnier, C., Antonijević-Miljaković, E., Mickova, V., Musilek, K.,& Zemanova, L.. (2024). Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta
Elsevier B.V.., 276.
https://doi.org/10.1016/j.talanta.2024.126263

Andrys R, Monnier C, Antonijević-Miljaković E, Mickova V, Musilek K, Zemanova L. Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta. 2024;276.
doi:10.1016/j.talanta.2024.126263 .

Andrys, Rudolf, Monnier, Charline, Antonijević-Miljaković, Evica, Mickova, Veronika, Musilek, Kamil, Zemanova, Lucie, "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research" in Talanta, 276 (2024),
https://doi.org/10.1016/j.talanta.2024.126263 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Marodi, Marko
AU  - Marković, Bojan
AU  - Tomašič, Tihomir
AU  - Durcik, Martina
AU  - Zidar, Nace
AU  - Mašič, Peterlin L.
AU  - Ilaš, Janez
AU  - Kikelj, Danijel
AU  - Čudina, Olivera
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5644
AB  - DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.
PB  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
T1  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
VL  - 1240
DO  - 10.1016/j.jchromb.2024.124158
ER  - 

@article{
author = "Dobričić, Vladimir and Marodi, Marko and Marković, Bojan and Tomašič, Tihomir and Durcik, Martina and Zidar, Nace and Mašič, Peterlin L. and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2024",
abstract = "DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.",
publisher = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
title = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
volume = "1240",
doi = "10.1016/j.jchromb.2024.124158"
}

Dobričić, V., Marodi, M., Marković, B., Tomašič, T., Durcik, M., Zidar, N., Mašič, P. L., Ilaš, J., Kikelj, D.,& Čudina, O.. (2024). Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis., 1240.
https://doi.org/10.1016/j.jchromb.2024.124158

Dobričić V, Marodi M, Marković B, Tomašič T, Durcik M, Zidar N, Mašič PL, Ilaš J, Kikelj D, Čudina O. Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2024;1240.
doi:10.1016/j.jchromb.2024.124158 .

Dobričić, Vladimir, Marodi, Marko, Marković, Bojan, Tomašič, Tihomir, Durcik, Martina, Zidar, Nace, Mašič, Peterlin L., Ilaš, Janez, Kikelj, Danijel, Čudina, Olivera, "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis" in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1240 (2024),
https://doi.org/10.1016/j.jchromb.2024.124158 . .

TY  - JOUR
AU  - Krmar, Jovana
AU  - Svrkota, Bojana
AU  - Obradović, Darija
AU  - Vlatković, Vladimir
AU  - Lazović, Saša
AU  - Otašević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5634
AB  - Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice.
AB  - Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis
T1  - Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi
VL  - 74
IS  - 2
SP  - 133
EP  - 159
DO  - 10.5937/arhfarm74-49565
ER  - 

@article{
author = "Krmar, Jovana and Svrkota, Bojana and Obradović, Darija and Vlatković, Vladimir and Lazović, Saša and Otašević, Biljana",
year = "2024",
abstract = "Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice., Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis, Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi",
volume = "74",
number = "2",
pages = "133-159",
doi = "10.5937/arhfarm74-49565"
}

Krmar, J., Svrkota, B., Obradović, D., Vlatković, V., Lazović, S.,& Otašević, B.. (2024). Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 133-159.
https://doi.org/10.5937/arhfarm74-49565

Krmar J, Svrkota B, Obradović D, Vlatković V, Lazović S, Otašević B. Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju. 2024;74(2):133-159.
doi:10.5937/arhfarm74-49565 .

Krmar, Jovana, Svrkota, Bojana, Obradović, Darija, Vlatković, Vladimir, Lazović, Saša, Otašević, Biljana, "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis" in Arhiv za farmaciju, 74, no. 2 (2024):133-159,
https://doi.org/10.5937/arhfarm74-49565 . .

TY  - JOUR
AU  - Đajić, Nevena
AU  - Krmar, Jovana
AU  - Stojanović, Jevrem
AU  - Svrkota, Bojana
AU  - Otašević, Biljana
AU  - Malenović, Anđelija
AU  - Protić, Ana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5640
AB  - Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion.
AB  - U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products
T1  - 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda
VL  - 74
IS  - 2
SP  - 235
EP  - 266
DO  - 10.5937/arhfarm74-49572
ER  - 

@article{
author = "Đajić, Nevena and Krmar, Jovana and Stojanović, Jevrem and Svrkota, Bojana and Otašević, Biljana and Malenović, Anđelija and Protić, Ana",
year = "2024",
abstract = "Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion., U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products, 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda",
volume = "74",
number = "2",
pages = "235-266",
doi = "10.5937/arhfarm74-49572"
}

Đajić, N., Krmar, J., Stojanović, J., Svrkota, B., Otašević, B., Malenović, A.,& Protić, A.. (2024). Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 235-266.
https://doi.org/10.5937/arhfarm74-49572

Đajić N, Krmar J, Stojanović J, Svrkota B, Otašević B, Malenović A, Protić A. Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju. 2024;74(2):235-266.
doi:10.5937/arhfarm74-49572 .

Đajić, Nevena, Krmar, Jovana, Stojanović, Jevrem, Svrkota, Bojana, Otašević, Biljana, Malenović, Anđelija, Protić, Ana, "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products" in Arhiv za farmaciju, 74, no. 2 (2024):235-266,
https://doi.org/10.5937/arhfarm74-49572 . .