TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Ocić, Gordana
AU  - Smiljković, Predrag
AU  - Siedlak, Sandra L.
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1370
AB  - Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
PB  - Oxford Univ Press Inc, Cary
T2  - Journals of Gerontology Series A: Biological Sciences and Medical Sciences
T1  - Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age
VL  - 65
IS  - 12
SP  - 1269
EP  - 1274
DO  - 10.1093/gerona/glq148
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Plećaš-Solarović, Bosiljka and Đelić, Ninoslav and Ocić, Gordana and Smiljković, Predrag and Siedlak, Sandra L. and Smith, Mark A. and Bajić, Vladan",
year = "2010",
abstract = "Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journals of Gerontology Series A: Biological Sciences and Medical Sciences",
title = "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age",
volume = "65",
number = "12",
pages = "1269-1274",
doi = "10.1093/gerona/glq148"
}

Živković, L., Potparević, B., Plećaš-Solarović, B., Đelić, N., Ocić, G., Smiljković, P., Siedlak, S. L., Smith, M. A.,& Bajić, V.. (2010). Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Oxford Univ Press Inc, Cary., 65(12), 1269-1274.
https://doi.org/10.1093/gerona/glq148

Živković L, Potparević B, Plećaš-Solarović B, Đelić N, Ocić G, Smiljković P, Siedlak SL, Smith MA, Bajić V. Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2010;65(12):1269-1274.
doi:10.1093/gerona/glq148 .

Živković, Lada, Potparević, Biljana, Plećaš-Solarović, Bosiljka, Đelić, Ninoslav, Ocić, Gordana, Smiljković, Predrag, Siedlak, Sandra L., Smith, Mark A., Bajić, Vladan, "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age" in Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 65, no. 12 (2010):1269-1274,
https://doi.org/10.1093/gerona/glq148 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Bonda, David J.
AU  - Siedlak, Sandra L.
AU  - Casadesus, Gemma
AU  - Lee, Hyoung-Gon
AU  - Smith, Mark A.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1212
AB  - Premature centromere division, or premature centromere separation (PCS), occurs when chromatid separation is dysfunctional, occurring earlier than usual during the interphase stage of mitosis. This phenomenon, seen in Robert's syndrome and various cancers, has also been documented in peripheral as well as neuronal cells of Alzheimer's disease (AD). In the latter instances, fluorescent in situ hybridization (FISH), applied to the centromere region of the X-chromosome in interphase nuclei of lymphocytes from peripheral blood in AD patients, demonstrated premature chromosomal separation before mitotic metaphase directly after completion of DNA replication in G(2) phase of the cell cycle. Furthermore, and perhaps unexpectedly given the presumptive post-mitotic status of terminally differentiated neurons, neurons in AD patients also showed significantly increased levels of PCS of the X-chromosome. Taken together with other phenomena such as cell cycle re-activation and ectopic re-expression of cyclins and cyclin dependent proteins, we propose that AD is an oncogenic phenotype leading to accelarated aging of the affected brain.
PB  - Churchill Livingstone, Edinburgh
T2  - Medical Hypotheses
T1  - The X-chromosome instability phenotype in Alzheimer's disease: A clinical sign of accelerating aging?
VL  - 73
IS  - 6
SP  - 917
EP  - 920
DO  - 10.1016/j.mehy.2009.06.046
ER  - 

@article{
author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Bonda, David J. and Siedlak, Sandra L. and Casadesus, Gemma and Lee, Hyoung-Gon and Smith, Mark A.",
year = "2009",
abstract = "Premature centromere division, or premature centromere separation (PCS), occurs when chromatid separation is dysfunctional, occurring earlier than usual during the interphase stage of mitosis. This phenomenon, seen in Robert's syndrome and various cancers, has also been documented in peripheral as well as neuronal cells of Alzheimer's disease (AD). In the latter instances, fluorescent in situ hybridization (FISH), applied to the centromere region of the X-chromosome in interphase nuclei of lymphocytes from peripheral blood in AD patients, demonstrated premature chromosomal separation before mitotic metaphase directly after completion of DNA replication in G(2) phase of the cell cycle. Furthermore, and perhaps unexpectedly given the presumptive post-mitotic status of terminally differentiated neurons, neurons in AD patients also showed significantly increased levels of PCS of the X-chromosome. Taken together with other phenomena such as cell cycle re-activation and ectopic re-expression of cyclins and cyclin dependent proteins, we propose that AD is an oncogenic phenotype leading to accelarated aging of the affected brain.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Medical Hypotheses",
title = "The X-chromosome instability phenotype in Alzheimer's disease: A clinical sign of accelerating aging?",
volume = "73",
number = "6",
pages = "917-920",
doi = "10.1016/j.mehy.2009.06.046"
}

Bajić, V., Potparević, B., Živković, L., Bonda, D. J., Siedlak, S. L., Casadesus, G., Lee, H.,& Smith, M. A.. (2009). The X-chromosome instability phenotype in Alzheimer's disease: A clinical sign of accelerating aging?. in Medical Hypotheses
Churchill Livingstone, Edinburgh., 73(6), 917-920.
https://doi.org/10.1016/j.mehy.2009.06.046

Bajić V, Potparević B, Živković L, Bonda DJ, Siedlak SL, Casadesus G, Lee H, Smith MA. The X-chromosome instability phenotype in Alzheimer's disease: A clinical sign of accelerating aging?. in Medical Hypotheses. 2009;73(6):917-920.
doi:10.1016/j.mehy.2009.06.046 .

Bajić, Vladan, Potparević, Biljana, Živković, Lada, Bonda, David J., Siedlak, Sandra L., Casadesus, Gemma, Lee, Hyoung-Gon, Smith, Mark A., "The X-chromosome instability phenotype in Alzheimer's disease: A clinical sign of accelerating aging?" in Medical Hypotheses, 73, no. 6 (2009):917-920,
https://doi.org/10.1016/j.mehy.2009.06.046 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1233
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3670
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3670"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Potparević, B., Živković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670

Bajić V, Stanimirović Z, Stevanović J, Potparević B, Živković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Potparević, Biljana, Živković, Lada, Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1072
AB  - 8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Physical Chemistry A
T1  - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
VL  - 44
IS  - 5
SP  - 546
EP  - 552
DO  - 10.1134/S1022795408050062
ER  - 

@article{
author = "Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2008",
abstract = "8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Physical Chemistry A",
title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro",
volume = "44",
number = "5",
pages = "546-552",
doi = "10.1134/S1022795408050062"
}

Bajić, V., Đelić, N., Potparević, B., Živković, L.,& Milicević, Z.. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A
Maik Nauka/Interperiodica/Springer, New York., 44(5), 546-552.
https://doi.org/10.1134/S1022795408050062

Bajić V, Đelić N, Potparević B, Živković L, Milicević Z. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A. 2008;44(5):546-552.
doi:10.1134/S1022795408050062 .

Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, Živković, Lada, Milicević, Z., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" in Russian Journal of Physical Chemistry A, 44, no. 5 (2008):546-552,
https://doi.org/10.1134/S1022795408050062 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Dačić, S.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1144
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro analysis of clastogenic effects of adrenaline on human lymphocytes
VL  - 60
IS  - 3
SP  - 15
EP  - 16
DO  - 10.2298/ABS0803015D
ER  - 

@article{
author = "Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Marković, Biljana and Dačić, S.",
year = "2008",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes",
volume = "60",
number = "3",
pages = "15-16",
doi = "10.2298/ABS0803015D"
}

Đelić, N., Potparević, B., Živković, L., Marković, B.,& Dačić, S.. (2008). In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 15-16.
https://doi.org/10.2298/ABS0803015D

Đelić N, Potparević B, Živković L, Marković B, Dačić S. In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences. 2008;60(3):15-16.
doi:10.2298/ABS0803015D .

Đelić, Ninoslav, Potparević, Biljana, Živković, Lada, Marković, Biljana, Dačić, S., "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes" in Archives of Biological Sciences, 60, no. 3 (2008):15-16,
https://doi.org/10.2298/ABS0803015D . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana J.
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Lozance, Olivera
AU  - Blagojević, Miloš
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/901
AB  - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
VL  - 30
IS  - 4
SP  - 1144
EP  - 1149
DO  - 10.1590/S1415-47572007000600019
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana J. and Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozance, Olivera and Blagojević, Miloš",
year = "2007",
abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes",
volume = "30",
number = "4",
pages = "1144-1149",
doi = "10.1590/S1415-47572007000600019"
}

Đelić, N., Đelić, D. J., Potparević, B., Živković, L., Marković, B., Lozance, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149.
https://doi.org/10.1590/S1415-47572007000600019

Đelić N, Đelić DJ, Potparević B, Živković L, Marković B, Lozance O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149.
doi:10.1590/S1415-47572007000600019 .

Đelić, Ninoslav, Đelić, Dijana J., Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozance, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149,
https://doi.org/10.1590/S1415-47572007000600019 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Marković, Biljana
AU  - Živković, Lada
AU  - Đelić, Dijana J.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/868
AB  - Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level.
AB  - Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro
T1  - Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro
VL  - 56
IS  - 1
SP  - 37
EP  - 48
DO  - 10.2298/AVB0601037D
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Marković, Biljana and Živković, Lada and Đelić, Dijana J.",
year = "2006",
abstract = "Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level., Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro, Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro",
volume = "56",
number = "1",
pages = "37-48",
doi = "10.2298/AVB0601037D"
}

Đelić, N., Spremo-Potparević, B., Marković, B., Živković, L.,& Đelić, D. J.. (2006). Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(1), 37-48.
https://doi.org/10.2298/AVB0601037D

Đelić N, Spremo-Potparević B, Marković B, Živković L, Đelić DJ. Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria. 2006;56(1):37-48.
doi:10.2298/AVB0601037D .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Marković, Biljana, Živković, Lada, Đelić, Dijana J., "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro" in Acta veterinaria, 56, no. 1 (2006):37-48,
https://doi.org/10.2298/AVB0601037D . .

TY  - JOUR
AU  - Đelić, N
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Đelić, D
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/851
AB  - Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.
PB  - Elsevier Science BV, Amsterdam
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro
VL  - 604
IS  - 1-2
SP  - 1
EP  - 7
DO  - 10.1016/j.mrgentox.2005.11.013
ER  - 

@article{
author = "Đelić, N and Potparević, Biljana and Bajić, Vladan and Đelić, D",
year = "2006",
abstract = "Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro",
volume = "604",
number = "1-2",
pages = "1-7",
doi = "10.1016/j.mrgentox.2005.11.013"
}

Đelić, N., Potparević, B., Bajić, V.,& Đelić, D.. (2006). Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier Science BV, Amsterdam., 604(1-2), 1-7.
https://doi.org/10.1016/j.mrgentox.2005.11.013

Đelić N, Potparević B, Bajić V, Đelić D. Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2006;604(1-2):1-7.
doi:10.1016/j.mrgentox.2005.11.013 .

Đelić, N, Potparević, Biljana, Bajić, Vladan, Đelić, D, "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 604, no. 1-2 (2006):1-7,
https://doi.org/10.1016/j.mrgentox.2005.11.013 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Đejić, Ninoslav
AU  - Bajić, Vladan
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/677
AB  - Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p  lt  0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.
PB  - Elsevier Ireland Ltd, Clare
T2  - Mechanisms of Ageing and Development
T1  - Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients
VL  - 127
IS  - 12
SP  - 892
EP  - 896
DO  - 10.1016/j.mad.2006.09.004
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Đejić, Ninoslav and Bajić, Vladan",
year = "2006",
abstract = "Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p  lt  0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Mechanisms of Ageing and Development",
title = "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients",
volume = "127",
number = "12",
pages = "892-896",
doi = "10.1016/j.mad.2006.09.004"
}

Živković, L., Potparević, B., Đejić, N.,& Bajić, V.. (2006). Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients. in Mechanisms of Ageing and Development
Elsevier Ireland Ltd, Clare., 127(12), 892-896.
https://doi.org/10.1016/j.mad.2006.09.004

Živković L, Potparević B, Đejić N, Bajić V. Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients. in Mechanisms of Ageing and Development. 2006;127(12):892-896.
doi:10.1016/j.mad.2006.09.004 .

Živković, Lada, Potparević, Biljana, Đejić, Ninoslav, Bajić, Vladan, "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients" in Mechanisms of Ageing and Development, 127, no. 12 (2006):892-896,
https://doi.org/10.1016/j.mad.2006.09.004 . .