TY  - JOUR
AU  - Račić, Anđelka
AU  - Jurišić-Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Vučen, Sonja
AU  - Micov, Ana
AU  - Stepanović-Petrović, Radica
AU  - Tomić, Maja
AU  - Pecikoza, Uroš
AU  - Bajac, Jelena
AU  - Krajišnik, Danina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5610
AB  - The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy
VL  - 655
SP  - 124033
DO  - 10.1016/j.ijpharm.2024.124033
ER  - 

@article{
author = "Račić, Anđelka and Jurišić-Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Vučen, Sonja and Micov, Ana and Stepanović-Petrović, Radica and Tomić, Maja and Pecikoza, Uroš and Bajac, Jelena and Krajišnik, Danina",
year = "2024",
abstract = "The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy",
volume = "655",
pages = "124033",
doi = "10.1016/j.ijpharm.2024.124033"
}

Račić, A., Jurišić-Dukovski, B., Lovrić, J., Dobričić, V., Vučen, S., Micov, A., Stepanović-Petrović, R., Tomić, M., Pecikoza, U., Bajac, J.,& Krajišnik, D.. (2024). Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics
Elsevier., 655, 124033.
https://doi.org/10.1016/j.ijpharm.2024.124033

Račić A, Jurišić-Dukovski B, Lovrić J, Dobričić V, Vučen S, Micov A, Stepanović-Petrović R, Tomić M, Pecikoza U, Bajac J, Krajišnik D. Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics. 2024;655:124033.
doi:10.1016/j.ijpharm.2024.124033 .

Račić, Anđelka, Jurišić-Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Vučen, Sonja, Micov, Ana, Stepanović-Petrović, Radica, Tomić, Maja, Pecikoza, Uroš, Bajac, Jelena, Krajišnik, Danina, "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy" in International Journal of Pharmaceutics, 655 (2024):124033,
https://doi.org/10.1016/j.ijpharm.2024.124033 . .

Krajišnik, D., Savić, S., Ilić, T.,& Savić, S.. (2023). Injectable products' bioequivalence assessment. in Time-Proof Perspectives on Bioequivalence
Nova Science Publishers, Inc.., 221-251.
https://hdl.handle.net/21.15107/rcub_farfar_5534

Krajišnik D, Savić S, Ilić T, Savić S. Injectable products' bioequivalence assessment. in Time-Proof Perspectives on Bioequivalence. 2023;:221-251.
https://hdl.handle.net/21.15107/rcub_farfar_5534 .

Krajišnik, Danina, Savić, Sanela, Ilić, Tanja, Savić, Snežana, "Injectable products' bioequivalence assessment" in Time-Proof Perspectives on Bioequivalence (2023):221-251,
https://hdl.handle.net/21.15107/rcub_farfar_5534 .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Pantelić, Ivana
AU  - Nikolić, Ines
AU  - Majhen, Dragomira
AU  - Stojić-Vukanić, Zorica
AU  - Savić, Snežana
AU  - Krajišnik, Danina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4960
AB  - Ever since the development of the first vaccine, vaccination has had the great impact on global health, leading to the decrease in the burden of numerous infectious diseases. However, there is a constant need to improve existing vaccines and develop new vaccination strategies and vaccine platforms that induce a broader immune response compared to traditional vaccines. Modern vaccines tend to rely on certain nanotechnology platforms but are still expected to be readily available and easy for large-scale manufacturing and to induce a durable immune response. In this review, we present an overview of the most promising nanoadjuvants and nanoparticulate delivery systems and discuss their benefits from tehchnological and immunological standpoints as well as their objective drawbacks and possible side effects. The presented nano alums, silica and clay nanoparticles, nanoemulsions, adenoviral-vectored systems, adeno-associated viral vectors, vesicular stomatitis viral vectors, lentiviral vectors, virus-like particles (including bacteriophage-based ones) and virosomes indicate that vaccine developers can now choose different adjuvants and/or delivery systems as per the requirement, specific to combatting different infectious diseases.
PB  - MDPI
T2  - Vaccines
T1  - Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines
VL  - 11
IS  - 7
DO  - 10.3390/vaccines11071172
ER  - 

@article{
author = "Filipić, Brankica and Pantelić, Ivana and Nikolić, Ines and Majhen, Dragomira and Stojić-Vukanić, Zorica and Savić, Snežana and Krajišnik, Danina",
year = "2023",
abstract = "Ever since the development of the first vaccine, vaccination has had the great impact on global health, leading to the decrease in the burden of numerous infectious diseases. However, there is a constant need to improve existing vaccines and develop new vaccination strategies and vaccine platforms that induce a broader immune response compared to traditional vaccines. Modern vaccines tend to rely on certain nanotechnology platforms but are still expected to be readily available and easy for large-scale manufacturing and to induce a durable immune response. In this review, we present an overview of the most promising nanoadjuvants and nanoparticulate delivery systems and discuss their benefits from tehchnological and immunological standpoints as well as their objective drawbacks and possible side effects. The presented nano alums, silica and clay nanoparticles, nanoemulsions, adenoviral-vectored systems, adeno-associated viral vectors, vesicular stomatitis viral vectors, lentiviral vectors, virus-like particles (including bacteriophage-based ones) and virosomes indicate that vaccine developers can now choose different adjuvants and/or delivery systems as per the requirement, specific to combatting different infectious diseases.",
publisher = "MDPI",
journal = "Vaccines",
title = "Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines",
volume = "11",
number = "7",
doi = "10.3390/vaccines11071172"
}

Filipić, B., Pantelić, I., Nikolić, I., Majhen, D., Stojić-Vukanić, Z., Savić, S.,& Krajišnik, D.. (2023). Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines. in Vaccines
MDPI., 11(7).
https://doi.org/10.3390/vaccines11071172

Filipić B, Pantelić I, Nikolić I, Majhen D, Stojić-Vukanić Z, Savić S, Krajišnik D. Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines. in Vaccines. 2023;11(7).
doi:10.3390/vaccines11071172 .

Filipić, Brankica, Pantelić, Ivana, Nikolić, Ines, Majhen, Dragomira, Stojić-Vukanić, Zorica, Savić, Snežana, Krajišnik, Danina, "Nanoparticle-Based Adjuvants and Delivery Systems for Modern Vaccines" in Vaccines, 11, no. 7 (2023),
https://doi.org/10.3390/vaccines11071172 . .

TY  - JOUR
AU  - Račić, Anđelka
AU  - Krajišnik, Danina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4518
AB  - Dry eye syndrome and allergic conjunctivitis are the most common inflammatory disorders of the eye surface. Although eye drops are the most usual prescribed dosage form, they are characterized by low ocular availability due to numerous barrier mechanisms of the eye. The use of biopolymers in liquid ophthalmic preparations has numerous advantages, such as increasing the viscosity of the tear film, exhibiting bioadhesive properties, and resisting the drainage system, leading to prolonged retention of the preparation at the site of application, and improvement of the therapeutic effect. Some mucoadhesive polymers are multifunctional excipients, so they act by different mechanisms on increasing the permeability of the cornea. Additionally, many hydrophilic biopolymers can also represent the active substances in artificial tear preparations, due to their lubrication and moisturizing effect. With the modification of conventional ophthalmic preparations, there is a need for development of new methods for their characterization. Numerous methods for the assessment of mucoadhesiveness have been suggested by the literature. This review gives an overview related to the development of mucoadhesive liquid ophthalmic formulations for the treatment of dry eye and allergic conditions.
PB  - MDPI
T2  - Pharmaceutics
T1  - Biopolymers in Mucoadhesive Eye Drops for Treatment of Dry Eye and Allergic Conditions: Application and Perspectives
VL  - 15
IS  - 2
DO  - 10.3390/pharmaceutics15020470
ER  - 

@article{
author = "Račić, Anđelka and Krajišnik, Danina",
year = "2023",
abstract = "Dry eye syndrome and allergic conjunctivitis are the most common inflammatory disorders of the eye surface. Although eye drops are the most usual prescribed dosage form, they are characterized by low ocular availability due to numerous barrier mechanisms of the eye. The use of biopolymers in liquid ophthalmic preparations has numerous advantages, such as increasing the viscosity of the tear film, exhibiting bioadhesive properties, and resisting the drainage system, leading to prolonged retention of the preparation at the site of application, and improvement of the therapeutic effect. Some mucoadhesive polymers are multifunctional excipients, so they act by different mechanisms on increasing the permeability of the cornea. Additionally, many hydrophilic biopolymers can also represent the active substances in artificial tear preparations, due to their lubrication and moisturizing effect. With the modification of conventional ophthalmic preparations, there is a need for development of new methods for their characterization. Numerous methods for the assessment of mucoadhesiveness have been suggested by the literature. This review gives an overview related to the development of mucoadhesive liquid ophthalmic formulations for the treatment of dry eye and allergic conditions.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Biopolymers in Mucoadhesive Eye Drops for Treatment of Dry Eye and Allergic Conditions: Application and Perspectives",
volume = "15",
number = "2",
doi = "10.3390/pharmaceutics15020470"
}

Račić, A.,& Krajišnik, D.. (2023). Biopolymers in Mucoadhesive Eye Drops for Treatment of Dry Eye and Allergic Conditions: Application and Perspectives. in Pharmaceutics
MDPI., 15(2).
https://doi.org/10.3390/pharmaceutics15020470

Račić A, Krajišnik D. Biopolymers in Mucoadhesive Eye Drops for Treatment of Dry Eye and Allergic Conditions: Application and Perspectives. in Pharmaceutics. 2023;15(2).
doi:10.3390/pharmaceutics15020470 .

Račić, Anđelka, Krajišnik, Danina, "Biopolymers in Mucoadhesive Eye Drops for Treatment of Dry Eye and Allergic Conditions: Application and Perspectives" in Pharmaceutics, 15, no. 2 (2023),
https://doi.org/10.3390/pharmaceutics15020470 . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Čalija, Bojan
AU  - Dobričić, Vladimir
AU  - Jurišić Dukovsk, Bisera
AU  - Lovrić, Jasmina
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4566
AB  - To improve ocular bioavailability of eye drops, viscosity-increasing polymers with improved
functional properties have been used (1,2). The aim of this study was formulation and evaluation of
viscous eye drops using vehicles containing hydroxypropyl guar gum (HPG) (0.25% w/v), sodium
hyaluronate (SH) (0.4% w/v), and their combination (HPG-SH) as carriers for olopatadine (0.1% w/v).
Physicochemical properties (appearance, clarity, pH, osmolality, viscosity) of the prepared
formulations were tested. The drug permeability was estimated in vitro using HCE-T cell-based models
and the parallel artificial membrane permeability assay (PAMPA). MTT cytotoxicity assay was
performed at the end of the permeability study. Physicochemical stability of the formulated eye drops
was tested during 12 months. An accelerated stability study was performed using the heating/cooling
cycles test. The clarity, pH and osmolality of all formulations were in acceptable range for ophthalmic
preparations. Formulation HPG-SH showed significantly higher viscosity (73.1 mPa·s) than
formulations with single polymer (7.4 mPa·s for HPG i.e 3.7 mPa·s for SH) pointing to synergistic effect
of polymers. The addition of polymers led to a decrease in transcorneal permeability and lower
permeability coefficients with prolonged residence of drug at the ocular surface. The results of MTT
assay demonstrated that the tested formulations were biocompatible and well tolerated. Formulated
eye drops showed satisfactory physicochemical stability under all storage conditions.
Olopatadine was successfully formulated in biocompatible viscous ophthalmic solutions. The
combination of viscosity enhancer HPG with mucoadhesive polymer SH has a potential to improve
precorneal residence time and therapeutic effect of olopatadine.
AB  - Polimeri poboljšanih funkcionalnih karakteristika koji povećavaju viskozitet koriste se u
kapima za oči kako bi se povećala okularna raspoloživost (1,2). Cilj ove studije bio je formulacija i
ispitivanje viskoznih kapi za oči korišćenjem vehikuluma koji sadrže hidroksipropil guar gumu (HPG)
(0,25% v/v), natrijum hijaluronat (SH) (0,4% v/v) i njihovu kombinaciju (HPG-SH) kao nosača za
olopatadin (0,1% v/v). Ispitivane su fizičkohemijske osobine (izgled, bistrina, pH, osmolalnost,
viskozitet) pripremljenih formulacija. Permeabilnost leka je procenjena in vitro korišćenjem modela
zasnovanih na HCE-T ćelijama i testa permeabilnosti na paralelnim veštačkim membranama (PAMPA).
MTT citotoksični test sproveden je na kraju studije permeabilnosti. Fizičkohemijska stabilnost
formulisanih kapi za oči ispitivana je tokom 12 meseci. Sprovedena je ubrzana studija stabilnosti
korišćenjem cikličnog testa grejanja/hlađenja. Bistrina, pH i osmolalnost svih formulacija bili su u
prihvatljivom opsegu za oftalmološke preparate. Formulacija HPG-SH je pokazala značajno viši
viskozitet (73,1 mPa·s) od formulacija sa pojedinačnim polimerom (7,4 mPa·s za HPG, tj. 3,7 mPa·s za
SH) ukazujući na sinergistički efekat polimera. Dodatak polimera je doveo do smanjenja
transkornealne permeabilnosti i nižih koeficijenata permeabilnosti uz produženo zadržavanje leka na
površini oka. Rezultati MTT testa su pokazali da su testirane formulacije biokompatibilne i dobro
podnošljive. Formulisane kapi za oči imale su zadovoljavajuću fizičkohemijsku stabilnost pri svim
uslovima čuvanja. Uspešno su formulisani biokompatibilni viskozni rastvori za oftalmološku primenu
sa olopatadinom. Kombinacija HPG kao sredstva za povećanje viskoziteta sa mukoadhezivnim
polimerom SH ima potencijal da poboljša prekornealno vreme zadržavanja i terapijski efekat
olopatadina.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine
T1  - Procena uticaja hidroksipropil guar gume i hijaluronske kiseline na fizičkohemijske i funkcionalne karakteristike oftalmoloških vehikuluma za olopatadin
VL  - 72
IS  - 4 suplement
SP  - S388
EP  - S389
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4566
ER  - 

@conference{
author = "Račić, Anđelka and Čalija, Bojan and Dobričić, Vladimir and Jurišić Dukovsk, Bisera and Lovrić, Jasmina and Krajišnik, Danina",
year = "2022",
abstract = "To improve ocular bioavailability of eye drops, viscosity-increasing polymers with improved
functional properties have been used (1,2). The aim of this study was formulation and evaluation of
viscous eye drops using vehicles containing hydroxypropyl guar gum (HPG) (0.25% w/v), sodium
hyaluronate (SH) (0.4% w/v), and their combination (HPG-SH) as carriers for olopatadine (0.1% w/v).
Physicochemical properties (appearance, clarity, pH, osmolality, viscosity) of the prepared
formulations were tested. The drug permeability was estimated in vitro using HCE-T cell-based models
and the parallel artificial membrane permeability assay (PAMPA). MTT cytotoxicity assay was
performed at the end of the permeability study. Physicochemical stability of the formulated eye drops
was tested during 12 months. An accelerated stability study was performed using the heating/cooling
cycles test. The clarity, pH and osmolality of all formulations were in acceptable range for ophthalmic
preparations. Formulation HPG-SH showed significantly higher viscosity (73.1 mPa·s) than
formulations with single polymer (7.4 mPa·s for HPG i.e 3.7 mPa·s for SH) pointing to synergistic effect
of polymers. The addition of polymers led to a decrease in transcorneal permeability and lower
permeability coefficients with prolonged residence of drug at the ocular surface. The results of MTT
assay demonstrated that the tested formulations were biocompatible and well tolerated. Formulated
eye drops showed satisfactory physicochemical stability under all storage conditions.
Olopatadine was successfully formulated in biocompatible viscous ophthalmic solutions. The
combination of viscosity enhancer HPG with mucoadhesive polymer SH has a potential to improve
precorneal residence time and therapeutic effect of olopatadine., Polimeri poboljšanih funkcionalnih karakteristika koji povećavaju viskozitet koriste se u
kapima za oči kako bi se povećala okularna raspoloživost (1,2). Cilj ove studije bio je formulacija i
ispitivanje viskoznih kapi za oči korišćenjem vehikuluma koji sadrže hidroksipropil guar gumu (HPG)
(0,25% v/v), natrijum hijaluronat (SH) (0,4% v/v) i njihovu kombinaciju (HPG-SH) kao nosača za
olopatadin (0,1% v/v). Ispitivane su fizičkohemijske osobine (izgled, bistrina, pH, osmolalnost,
viskozitet) pripremljenih formulacija. Permeabilnost leka je procenjena in vitro korišćenjem modela
zasnovanih na HCE-T ćelijama i testa permeabilnosti na paralelnim veštačkim membranama (PAMPA).
MTT citotoksični test sproveden je na kraju studije permeabilnosti. Fizičkohemijska stabilnost
formulisanih kapi za oči ispitivana je tokom 12 meseci. Sprovedena je ubrzana studija stabilnosti
korišćenjem cikličnog testa grejanja/hlađenja. Bistrina, pH i osmolalnost svih formulacija bili su u
prihvatljivom opsegu za oftalmološke preparate. Formulacija HPG-SH je pokazala značajno viši
viskozitet (73,1 mPa·s) od formulacija sa pojedinačnim polimerom (7,4 mPa·s za HPG, tj. 3,7 mPa·s za
SH) ukazujući na sinergistički efekat polimera. Dodatak polimera je doveo do smanjenja
transkornealne permeabilnosti i nižih koeficijenata permeabilnosti uz produženo zadržavanje leka na
površini oka. Rezultati MTT testa su pokazali da su testirane formulacije biokompatibilne i dobro
podnošljive. Formulisane kapi za oči imale su zadovoljavajuću fizičkohemijsku stabilnost pri svim
uslovima čuvanja. Uspešno su formulisani biokompatibilni viskozni rastvori za oftalmološku primenu
sa olopatadinom. Kombinacija HPG kao sredstva za povećanje viskoziteta sa mukoadhezivnim
polimerom SH ima potencijal da poboljša prekornealno vreme zadržavanja i terapijski efekat
olopatadina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine, Procena uticaja hidroksipropil guar gume i hijaluronske kiseline na fizičkohemijske i funkcionalne karakteristike oftalmoloških vehikuluma za olopatadin",
volume = "72",
number = "4 suplement",
pages = "S388-S389",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4566"
}

Račić, A., Čalija, B., Dobričić, V., Jurišić Dukovsk, B., Lovrić, J.,& Krajišnik, D.. (2022). Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S388-S389.
https://hdl.handle.net/21.15107/rcub_farfar_4566

Račić A, Čalija B, Dobričić V, Jurišić Dukovsk B, Lovrić J, Krajišnik D. Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine. in Arhiv za farmaciju. 2022;72(4 suplement):S388-S389.
https://hdl.handle.net/21.15107/rcub_farfar_4566 .

Račić, Anđelka, Čalija, Bojan, Dobričić, Vladimir, Jurišić Dukovsk, Bisera, Lovrić, Jasmina, Krajišnik, Danina, "Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S388-S389,
https://hdl.handle.net/21.15107/rcub_farfar_4566 .

TY  - CONF
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Čalija, Bojan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4565
AB  - Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications.
AB  - Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites
T1  - Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance
VL  - 72
IS  - 4 suplement
SP  - S384
EP  - S385
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4565
ER  - 

@conference{
author = "Jauković, Valentina and Krajišnik, Danina and Čalija, Bojan",
year = "2022",
abstract = "Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications., Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites, Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance",
volume = "72",
number = "4 suplement",
pages = "S384-S385",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4565"
}

Jauković, V., Krajišnik, D.,& Čalija, B.. (2022). Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565

Jauković V, Krajišnik D, Čalija B. Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju. 2022;72(4 suplement):S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

Jauković, Valentina, Krajišnik, Danina, Čalija, Bojan, "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S384-S385,
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

TY  - JOUR
AU  - Martić, Radava
AU  - Kotur-Stevuljević, Jelena
AU  - Malenović, Anđelija
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4200
AB  - Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
VL  - 17
IS  - 7
DO  - 10.1177/1934578X221112811
ER  - 

@article{
author = "Martić, Radava and Kotur-Stevuljević, Jelena and Malenović, Anđelija and Ušjak, Ljuboš and Petrović, Silvana and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2022",
abstract = "Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment",
volume = "17",
number = "7",
doi = "10.1177/1934578X221112811"
}

Martić, R., Kotur-Stevuljević, J., Malenović, A., Ušjak, L., Petrović, S., Čalija, B., Milić, J.,& Krajišnik, D.. (2022). Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications
SAGE Publications Inc.., 17(7).
https://doi.org/10.1177/1934578X221112811

Martić R, Kotur-Stevuljević J, Malenović A, Ušjak L, Petrović S, Čalija B, Milić J, Krajišnik D. Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications. 2022;17(7).
doi:10.1177/1934578X221112811 .

Martić, Radava, Kotur-Stevuljević, Jelena, Malenović, Anđelija, Ušjak, Ljuboš, Petrović, Silvana, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment" in Natural Product Communications, 17, no. 7 (2022),
https://doi.org/10.1177/1934578X221112811 . .

TY  - JOUR
AU  - Popadić, Daliborka
AU  - Gavrilov, Nemanja
AU  - Ignjatović, Ljubiša
AU  - Krajišnik, Danina
AU  - Mentus, Slavko
AU  - Milojević-Rakić, Maja
AU  - Bajuk-Bogdanović, Danica
PY  - 2022
UR  - https://www.mdpi.com/2073-4344/12/5/519
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4099
AB  - Unmodified natural silicates (bentonite, kaolin, clinoptilolite and diatomites) were tested as adsorbents for the organic pollutants in water tables using Methylene Blue (MB) as the model adsorbate. Among the selected materials, bentonite adsorbed as much as 237 mg/g, confirming its excellent suitability for pollutant removal. Spectral evidence confirmed successful MB immobilization at the bentonite surface. Furthermore, the thermal treatment of MB-saturated adsorbent in an inert atmosphere at 700 °C produced a carbon/silicate composite. EDX confirmed the formation of the nitrogen-doped carbon overlay on the silica scaffold and the obtained composite material was probed as an electrode material for oxygen reduction in an alkaline solution. Reduction proceeded via a two-electron mechanism with the main product being HO2−, a known nucleophile, which was subsequently used to degrade/demethylate MB. The composite showed a considerable 70% MB removal rate after an hour of electrochemical treatment. The synergy between the processes of adsorption of MB and the surface-generated HO2− dictates the efficiency of the method and points to a possible route for spent adsorbent reuse in the form of a durable product for environmental protection.
PB  - MDPI
T2  - Catalysts
T1  - How to Obtain Maximum Environmental Applicability from Natural Silicates
VL  - 12
IS  - 5
DO  - 10.3390/catal12050519
ER  - 

@article{
author = "Popadić, Daliborka and Gavrilov, Nemanja and Ignjatović, Ljubiša and Krajišnik, Danina and Mentus, Slavko and Milojević-Rakić, Maja and Bajuk-Bogdanović, Danica",
year = "2022",
abstract = "Unmodified natural silicates (bentonite, kaolin, clinoptilolite and diatomites) were tested as adsorbents for the organic pollutants in water tables using Methylene Blue (MB) as the model adsorbate. Among the selected materials, bentonite adsorbed as much as 237 mg/g, confirming its excellent suitability for pollutant removal. Spectral evidence confirmed successful MB immobilization at the bentonite surface. Furthermore, the thermal treatment of MB-saturated adsorbent in an inert atmosphere at 700 °C produced a carbon/silicate composite. EDX confirmed the formation of the nitrogen-doped carbon overlay on the silica scaffold and the obtained composite material was probed as an electrode material for oxygen reduction in an alkaline solution. Reduction proceeded via a two-electron mechanism with the main product being HO2−, a known nucleophile, which was subsequently used to degrade/demethylate MB. The composite showed a considerable 70% MB removal rate after an hour of electrochemical treatment. The synergy between the processes of adsorption of MB and the surface-generated HO2− dictates the efficiency of the method and points to a possible route for spent adsorbent reuse in the form of a durable product for environmental protection.",
publisher = "MDPI",
journal = "Catalysts",
title = "How to Obtain Maximum Environmental Applicability from Natural Silicates",
volume = "12",
number = "5",
doi = "10.3390/catal12050519"
}

Popadić, D., Gavrilov, N., Ignjatović, L., Krajišnik, D., Mentus, S., Milojević-Rakić, M.,& Bajuk-Bogdanović, D.. (2022). How to Obtain Maximum Environmental Applicability from Natural Silicates. in Catalysts
MDPI., 12(5).
https://doi.org/10.3390/catal12050519

Popadić D, Gavrilov N, Ignjatović L, Krajišnik D, Mentus S, Milojević-Rakić M, Bajuk-Bogdanović D. How to Obtain Maximum Environmental Applicability from Natural Silicates. in Catalysts. 2022;12(5).
doi:10.3390/catal12050519 .

Popadić, Daliborka, Gavrilov, Nemanja, Ignjatović, Ljubiša, Krajišnik, Danina, Mentus, Slavko, Milojević-Rakić, Maja, Bajuk-Bogdanović, Danica, "How to Obtain Maximum Environmental Applicability from Natural Silicates" in Catalysts, 12, no. 5 (2022),
https://doi.org/10.3390/catal12050519 . .

TY  - JOUR
AU  - Cvijić, Sandra
AU  - Mirković, Dušica
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4260
AB  - The treatment of respiratory infections in children requires special attention, since the
paediatric population has rather specific characteristics and consists of heterogenous subgroups.
In this context, the choice of a suitable drug dosage form is of particular importance, depending
on the active substance properties, along with the age and general condition of a paediatric patient.
Тhe most commonly used pharmaceutical products for respiratory infections in children include
oral, parenteral and inhalation dosage forms, although a large number of drugs are not available
in a suitable dosage form and/or strength for paediatric age, leading to the frequent use of
unauthorized drugs (i.e., unlicensed use). Other important issues that should be considered when
choosing the appropriate paediatric dosage form and/or compounding procedure are related to the
careful considerations of the pharmaceutical product composition (safety of excipients) and the
choice of administration/dosing device in relation to a child’s age.
This paper provides an overview of paediatric dosage forms used in the treatment of
respiratory infections in children, their benefits and limitations. The review includes examples of
various pharmaceutical products, along with the considerations regarding administration/dosing
devices. Specific characteristics of paediatric populations affecting the decision on the choice of
age-appropriate paediatric formulation are also addressed.
AB  - Terapija respiratornih infekcija kod dece zahteva posebnu pažnju, jer ovu populaciju čini specifična i izuzetno heterogena grupa pacijenata. Veoma je važno odabrati odgovarajući farmaceutski oblik leka, u skladu sa karakteristikama aktivne supstance, kao i stanjem i uzrastom deteta. U praksi se, u terapiji respiratornih infekcija kod dece, najviše koriste preparati za oralnu primenu, za parenteralnu primenu i za inhalaciju. Međutim, veliki broj lekova nije dostupan u odgovarajućem farmaceutskom obliku i/ili jačini za pedijatrijski uzrast, usled čega je česta neodobrena upotreba lekova. Prilikom izbora/izrade preparata za decu potrebno je pažljivo razmotriti i sastav preparata (bezbednost pomoćnih supstanci), kao i izbor aplikatora, u skladu sa uzrastom deteta. U ovom radu je dat prikaz farmaceutskih oblika lekova koji se koriste u terapiji respiratornih infekcija kod dece, njihovih prednosti i izvesnih nedostataka. Navedeni su različiti primeri farmaceutskih preparata, uz poseban osvrt na izbor aplikatora za primenu/doziranje lekova. Takođe, diskutovane su specifičnosti pedijatrijske populacije koje utiču na izbor odgovarajuće formulacije leka prilagođene uzrastu deteta.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - How to choose an appropriate drug dosage form for the treatment of respiratory infections in children: Facts and tips
T1  - Stavovi i saveti vezani za izbor farmaceutskih oblika lekova za lečenje respiratornih infekcija kod dece
VL  - 72
IS  - 3
SP  - 353
EP  - 372
DO  - 10.5937/arhfarm72-37643
ER  - 

@article{
author = "Cvijić, Sandra and Mirković, Dušica and Krajišnik, Danina",
year = "2022",
abstract = "The treatment of respiratory infections in children requires special attention, since the
paediatric population has rather specific characteristics and consists of heterogenous subgroups.
In this context, the choice of a suitable drug dosage form is of particular importance, depending
on the active substance properties, along with the age and general condition of a paediatric patient.
Тhe most commonly used pharmaceutical products for respiratory infections in children include
oral, parenteral and inhalation dosage forms, although a large number of drugs are not available
in a suitable dosage form and/or strength for paediatric age, leading to the frequent use of
unauthorized drugs (i.e., unlicensed use). Other important issues that should be considered when
choosing the appropriate paediatric dosage form and/or compounding procedure are related to the
careful considerations of the pharmaceutical product composition (safety of excipients) and the
choice of administration/dosing device in relation to a child’s age.
This paper provides an overview of paediatric dosage forms used in the treatment of
respiratory infections in children, their benefits and limitations. The review includes examples of
various pharmaceutical products, along with the considerations regarding administration/dosing
devices. Specific characteristics of paediatric populations affecting the decision on the choice of
age-appropriate paediatric formulation are also addressed., Terapija respiratornih infekcija kod dece zahteva posebnu pažnju, jer ovu populaciju čini specifična i izuzetno heterogena grupa pacijenata. Veoma je važno odabrati odgovarajući farmaceutski oblik leka, u skladu sa karakteristikama aktivne supstance, kao i stanjem i uzrastom deteta. U praksi se, u terapiji respiratornih infekcija kod dece, najviše koriste preparati za oralnu primenu, za parenteralnu primenu i za inhalaciju. Međutim, veliki broj lekova nije dostupan u odgovarajućem farmaceutskom obliku i/ili jačini za pedijatrijski uzrast, usled čega je česta neodobrena upotreba lekova. Prilikom izbora/izrade preparata za decu potrebno je pažljivo razmotriti i sastav preparata (bezbednost pomoćnih supstanci), kao i izbor aplikatora, u skladu sa uzrastom deteta. U ovom radu je dat prikaz farmaceutskih oblika lekova koji se koriste u terapiji respiratornih infekcija kod dece, njihovih prednosti i izvesnih nedostataka. Navedeni su različiti primeri farmaceutskih preparata, uz poseban osvrt na izbor aplikatora za primenu/doziranje lekova. Takođe, diskutovane su specifičnosti pedijatrijske populacije koje utiču na izbor odgovarajuće formulacije leka prilagođene uzrastu deteta.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "How to choose an appropriate drug dosage form for the treatment of respiratory infections in children: Facts and tips, Stavovi i saveti vezani za izbor farmaceutskih oblika lekova za lečenje respiratornih infekcija kod dece",
volume = "72",
number = "3",
pages = "353-372",
doi = "10.5937/arhfarm72-37643"
}

Cvijić, S., Mirković, D.,& Krajišnik, D.. (2022). How to choose an appropriate drug dosage form for the treatment of respiratory infections in children: Facts and tips. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 72(3), 353-372.
https://doi.org/10.5937/arhfarm72-37643

Cvijić S, Mirković D, Krajišnik D. How to choose an appropriate drug dosage form for the treatment of respiratory infections in children: Facts and tips. in Arhiv za farmaciju. 2022;72(3):353-372.
doi:10.5937/arhfarm72-37643 .

Cvijić, Sandra, Mirković, Dušica, Krajišnik, Danina, "How to choose an appropriate drug dosage form for the treatment of respiratory infections in children: Facts and tips" in Arhiv za farmaciju, 72, no. 3 (2022):353-372,
https://doi.org/10.5937/arhfarm72-37643 . .

TY  - CONF
AU  - Ivković, Branka
AU  - Božić, Dragana
AU  - Kotur-Stevuljević, Jelena
AU  - Krajišnik, Danina
AU  - Vujić, Zorica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4598
AB  - The global market for antiseptics and disinfectants is growing rapidly, fueled by a
coronavirus pandemic and a possible outbreak of infectious diseases in the future. The
antimicrobial, anti-inflammatory and redox activity of chalcone (1,3-diaryl-2-propen-1-one)
is well documented in the literature. The aim of this study is screening of antimicrobial
activity of chalcones and their saturated derivatives as active pharmaceutical ingredients,
synthesized by Claisen-Schmidt condensation (1). The redox potential of the investigated
compounds was tested in the biological environment using spectrophotometric methods to
determine prooxidant/antioxidant parameters. Within preformulation studies solubility and
compatibility with excipients commonly used in liquid pharmaceutical dosage forms was
performed. All the tested chalcones and their saturated derivatives showed satisfactory
antimicrobial activity, but two saturated chalcones showed the best MIC (0.156 - 1.25 mM)
and were categorized as compounds with strong bacteriostatic activity (2). Solubility of the
chalcons with moderate antimicrobial activity and redox potential was higher than its
minimum bacteriostatic and bactericidal concentration in all the tested solvents (ethanol,
isopropyl alcohol and propylene glycol). Based on the chemical structure and predicted logP
values for saturated chalcones that show stronger antimicrobial activity, better water
solubility is expected. These data could be a starting point for formulations of antiseptics and
disinfectants with lower concentrations of alcohol-based solvents, as the synergism between
saturated chalcone and ethanol and isopropyl alcohol was previously shown. Considering the
lighter color of the saturated chalcone, better aesthetic acceptability of the developed
formulations is expected, since it will not stain the skin and objects during application.
AB  - Globalno tržište antiseptika i dezinfekcionih sredstava ubrzano raste podstaknuto
pandemijom koronavirusa i moguć im širenjem drugih zaraznih bolesti u buduć nosti.
Antimikrobna, antiinflamatorna i redoks aktivnost halkona (1,3-diaril-2-propen-1-ona) je
dobro dokumentovana u literaturi. Cilj ove studije bio je skrining antimikrobne aktivnosti
halkona i njihovih zasić enih derivata kao aktivnih farmaceutskih sastojaka, sintetisanih
Claisen‐Schmidt kondenzacijom (1). Redoks potencijal ispitivanih jedinjenja je ispitan u
biosredini primenom spektrofotometrijskih metoda za određivanje
prooksidantnih/antioksidativnih parametara. Sprovedena su i preformulaciona ispitivanja
rastvorljivosti i kompatibilnosti sa ekscipijensima uobičajeno korišćenim u tečnim
farmaceutskim oblicima. Svi testirani halkoni i njihovi zasić eni derivati su pokazali
zadovoljavajuć u antimikrobnu aktivnost, ali su dva zasić ena halkona pokazala najbolji MIC
(0,156 – 1,25 mM) i kategorisana su kao jedinjenja sa jakom bakteriostatskom aktivnošć u
(2). Rastvorljivost halkona sa umerenom antimikrobnom aktivnošću i redoks potencijalom
bili su viši od njegove minimalne bakteriostatske i baktericidne koncentracije u svim
ispitivanim rastvaračima (etanol, izopropil alkohol i propilenglikol). Na osnovu hemijske
strukture i predviđene logP vrednosti za zasićene halkone koji pokazuju bolju antimikrobnu
aktivnost, očekuje se i bolja rastvorljivost u vodi. Ovi podaci mogli bi da budu polazna osnova
za formulisanje antiseptika i dezinfekcionih sredstava sa nižim koncentracijama rastvarača
na bazi alkohola, uzimajući u obzir da je u prethodnom ispitivanju pokazan sinergizam
između zasićenog halkona i etanola, kao i izopropil alkohola. S obzirom na svetliju boju
zasićenog halkona, očekuje se i bolja estetska prihvatljivost razvijenih formulacija, jer neć e
bojiti kožu i predmete tokom nanošenja.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chalcone derivatives as potential antiseptics and disinfectants
T1  - Derivati halkona kao potentijalni antiseptici i dezinficijensi
VL  - 72
IS  - 4 suplement
SP  - S532
EP  - S533
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4598
ER  - 

@conference{
author = "Ivković, Branka and Božić, Dragana and Kotur-Stevuljević, Jelena and Krajišnik, Danina and Vujić, Zorica",
year = "2022",
abstract = "The global market for antiseptics and disinfectants is growing rapidly, fueled by a
coronavirus pandemic and a possible outbreak of infectious diseases in the future. The
antimicrobial, anti-inflammatory and redox activity of chalcone (1,3-diaryl-2-propen-1-one)
is well documented in the literature. The aim of this study is screening of antimicrobial
activity of chalcones and their saturated derivatives as active pharmaceutical ingredients,
synthesized by Claisen-Schmidt condensation (1). The redox potential of the investigated
compounds was tested in the biological environment using spectrophotometric methods to
determine prooxidant/antioxidant parameters. Within preformulation studies solubility and
compatibility with excipients commonly used in liquid pharmaceutical dosage forms was
performed. All the tested chalcones and their saturated derivatives showed satisfactory
antimicrobial activity, but two saturated chalcones showed the best MIC (0.156 - 1.25 mM)
and were categorized as compounds with strong bacteriostatic activity (2). Solubility of the
chalcons with moderate antimicrobial activity and redox potential was higher than its
minimum bacteriostatic and bactericidal concentration in all the tested solvents (ethanol,
isopropyl alcohol and propylene glycol). Based on the chemical structure and predicted logP
values for saturated chalcones that show stronger antimicrobial activity, better water
solubility is expected. These data could be a starting point for formulations of antiseptics and
disinfectants with lower concentrations of alcohol-based solvents, as the synergism between
saturated chalcone and ethanol and isopropyl alcohol was previously shown. Considering the
lighter color of the saturated chalcone, better aesthetic acceptability of the developed
formulations is expected, since it will not stain the skin and objects during application., Globalno tržište antiseptika i dezinfekcionih sredstava ubrzano raste podstaknuto
pandemijom koronavirusa i moguć im širenjem drugih zaraznih bolesti u buduć nosti.
Antimikrobna, antiinflamatorna i redoks aktivnost halkona (1,3-diaril-2-propen-1-ona) je
dobro dokumentovana u literaturi. Cilj ove studije bio je skrining antimikrobne aktivnosti
halkona i njihovih zasić enih derivata kao aktivnih farmaceutskih sastojaka, sintetisanih
Claisen‐Schmidt kondenzacijom (1). Redoks potencijal ispitivanih jedinjenja je ispitan u
biosredini primenom spektrofotometrijskih metoda za određivanje
prooksidantnih/antioksidativnih parametara. Sprovedena su i preformulaciona ispitivanja
rastvorljivosti i kompatibilnosti sa ekscipijensima uobičajeno korišćenim u tečnim
farmaceutskim oblicima. Svi testirani halkoni i njihovi zasić eni derivati su pokazali
zadovoljavajuć u antimikrobnu aktivnost, ali su dva zasić ena halkona pokazala najbolji MIC
(0,156 – 1,25 mM) i kategorisana su kao jedinjenja sa jakom bakteriostatskom aktivnošć u
(2). Rastvorljivost halkona sa umerenom antimikrobnom aktivnošću i redoks potencijalom
bili su viši od njegove minimalne bakteriostatske i baktericidne koncentracije u svim
ispitivanim rastvaračima (etanol, izopropil alkohol i propilenglikol). Na osnovu hemijske
strukture i predviđene logP vrednosti za zasićene halkone koji pokazuju bolju antimikrobnu
aktivnost, očekuje se i bolja rastvorljivost u vodi. Ovi podaci mogli bi da budu polazna osnova
za formulisanje antiseptika i dezinfekcionih sredstava sa nižim koncentracijama rastvarača
na bazi alkohola, uzimajući u obzir da je u prethodnom ispitivanju pokazan sinergizam
između zasićenog halkona i etanola, kao i izopropil alkohola. S obzirom na svetliju boju
zasićenog halkona, očekuje se i bolja estetska prihvatljivost razvijenih formulacija, jer neć e
bojiti kožu i predmete tokom nanošenja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chalcone derivatives as potential antiseptics and disinfectants, Derivati halkona kao potentijalni antiseptici i dezinficijensi",
volume = "72",
number = "4 suplement",
pages = "S532-S533",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4598"
}

Ivković, B., Božić, D., Kotur-Stevuljević, J., Krajišnik, D.,& Vujić, Z.. (2022). Chalcone derivatives as potential antiseptics and disinfectants. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S532-S533.
https://hdl.handle.net/21.15107/rcub_farfar_4598

Ivković B, Božić D, Kotur-Stevuljević J, Krajišnik D, Vujić Z. Chalcone derivatives as potential antiseptics and disinfectants. in Arhiv za farmaciju. 2022;72(4 suplement):S532-S533.
https://hdl.handle.net/21.15107/rcub_farfar_4598 .

Ivković, Branka, Božić, Dragana, Kotur-Stevuljević, Jelena, Krajišnik, Danina, Vujić, Zorica, "Chalcone derivatives as potential antiseptics and disinfectants" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S532-S533,
https://hdl.handle.net/21.15107/rcub_farfar_4598 .

TY  - CONF
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4462
AB  - Drug delivery to the eye is still one of the most important areas of modern ocular
therapy, since it encompasses many opportunities and challenges. Traditional use of
polymers (both natural and synthetic) as excipients in ophthalmic preparations provides
better bioavailability by drug stabilization, viscosity increasing, mucoadhesion and retained
elimination rate from the eye, which reduces the frequency of drug administration and
enhances patient compliance. Furthermore, some polymers have properties of ocular
penetration enhancers or enable the formation of in situ gels in contact with various
physiological stimuli (ions, pH or temperature) (1). The versatility of biopolymers and
synthetic polymers have been applied for formulation of various micro- and nanocarriers
(e.g., microparticles, microneedles, nanoparticles, nanocapsules, polymeric micelles,
dendrimers, and liposomes) which demonstrate significant potential for treatment of ocular
diseases in both anterior and posterior segment of the eye. Processing polymers into fibers,
films, contact lenses, or extruded forms allows design of specifically engineered devices
enabling accuracy of dosing, prolonged drug release, chemical stability, and absence of
preservatives. Although significant effort has been devoted to development of polymer-
based ocular drug delivery systems, a key challenge is still the translation of these systems to
clinical use (2). Biocompatibility is an essential property for all ocular drug delivery systems
since their components should not interact with the surrounding tissue or elicit foreign body
reactions through inflammatory or immune response (1). Overall, despite numerous
challenges, further investigative research emerging innovative applications of polymeric
materials in ocular drug delivery is expected in the near future.
AB  - Isporuka leka u oko je i dalje jedna od najvažnijih oblasti savremene oftalmološke
terapije jer obuhvata mnoge moguć nosti i izazove. Tradicionalna upotreba polimera
(prirodnog i sintetskog porekla) kao ekscipijenata u oftalmološkim preparatima obezbeđuje
bolju biološku raspoloživost stabilizacijom leka, povećanjem viskoziteta, mukoadhezijom i
usporavanjem eliminacije leka iz oka, što dovodi do smanjenja učestalost primene leka i
poboljšanja komplijanse. Dodatno, neki od predstavnika polimera imaju svojstva pojačivača
okularne penetracije ili omoguć avaju formiranje in situ gelova u kontaktu sa različitim
fiziološkim stimulusima (prisustvo određenih jona, pH sredine ili temperatura) (1).
Mnogobrojne povoljne osobine biopolimera i sintetskih polimera primenjene su tokom
formulacije različitih mikro- i nanonosača (kao što su mikročestice, mikroigle, nanočestice,
nanokapsule, polimerne micele, dendrimeri i liposomi) koji pokazuju značajan potencijal za
lečenje oftalmoloških oboljenja, kako prednjeg prednjeg, tako i zadnjeg segmenta oka.
Prevođenje polimera u vlakna, filmove, kontaktna sočiva ili ekstrudirane oblike omogućava
dobijanje različitih specifično dizajniranih terapijskih sistema, koji omoguć avaju precizno
doziranje, produženo oslobađanje lekovite supstance, hemijsku stabilnost i odsustvo
konzervansa. Iako je uložen značajan napor u okviru razvoja oftalmoloških terapijskih
sistema, ključni izazov je još njihova primena u kliničkoj praksi (2). Osnovni zahtev koji ovi
sistemi moraju ispuniti odnosi se na biokompatibilnost, jer njihove komponente ne bi trebalo
da stupaju u interakciju sa okolnim tkivom ili da izazovu reakcije stranog tela putem
inflamatornog ili imunološkog odgovora (1). Konačno, uprkos brojnim izazovima, dalja
istraživanja usmerena ka razvoju inovativne primene polimernih materijala za oftalmološku
primenu očekuju se u bliskoj budućnosti.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Polymers in ophthalmic preparations: from traditional application to product innovation
T1  - Polimeri u oftalmološkim preparatima: od tradicionalne primene do inovacije proizvoda
VL  - 72
IS  - 4 suplement
SP  - 113
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4462
ER  - 

@conference{
author = "Krajišnik, Danina",
year = "2022",
abstract = "Drug delivery to the eye is still one of the most important areas of modern ocular
therapy, since it encompasses many opportunities and challenges. Traditional use of
polymers (both natural and synthetic) as excipients in ophthalmic preparations provides
better bioavailability by drug stabilization, viscosity increasing, mucoadhesion and retained
elimination rate from the eye, which reduces the frequency of drug administration and
enhances patient compliance. Furthermore, some polymers have properties of ocular
penetration enhancers or enable the formation of in situ gels in contact with various
physiological stimuli (ions, pH or temperature) (1). The versatility of biopolymers and
synthetic polymers have been applied for formulation of various micro- and nanocarriers
(e.g., microparticles, microneedles, nanoparticles, nanocapsules, polymeric micelles,
dendrimers, and liposomes) which demonstrate significant potential for treatment of ocular
diseases in both anterior and posterior segment of the eye. Processing polymers into fibers,
films, contact lenses, or extruded forms allows design of specifically engineered devices
enabling accuracy of dosing, prolonged drug release, chemical stability, and absence of
preservatives. Although significant effort has been devoted to development of polymer-
based ocular drug delivery systems, a key challenge is still the translation of these systems to
clinical use (2). Biocompatibility is an essential property for all ocular drug delivery systems
since their components should not interact with the surrounding tissue or elicit foreign body
reactions through inflammatory or immune response (1). Overall, despite numerous
challenges, further investigative research emerging innovative applications of polymeric
materials in ocular drug delivery is expected in the near future., Isporuka leka u oko je i dalje jedna od najvažnijih oblasti savremene oftalmološke
terapije jer obuhvata mnoge moguć nosti i izazove. Tradicionalna upotreba polimera
(prirodnog i sintetskog porekla) kao ekscipijenata u oftalmološkim preparatima obezbeđuje
bolju biološku raspoloživost stabilizacijom leka, povećanjem viskoziteta, mukoadhezijom i
usporavanjem eliminacije leka iz oka, što dovodi do smanjenja učestalost primene leka i
poboljšanja komplijanse. Dodatno, neki od predstavnika polimera imaju svojstva pojačivača
okularne penetracije ili omoguć avaju formiranje in situ gelova u kontaktu sa različitim
fiziološkim stimulusima (prisustvo određenih jona, pH sredine ili temperatura) (1).
Mnogobrojne povoljne osobine biopolimera i sintetskih polimera primenjene su tokom
formulacije različitih mikro- i nanonosača (kao što su mikročestice, mikroigle, nanočestice,
nanokapsule, polimerne micele, dendrimeri i liposomi) koji pokazuju značajan potencijal za
lečenje oftalmoloških oboljenja, kako prednjeg prednjeg, tako i zadnjeg segmenta oka.
Prevođenje polimera u vlakna, filmove, kontaktna sočiva ili ekstrudirane oblike omogućava
dobijanje različitih specifično dizajniranih terapijskih sistema, koji omoguć avaju precizno
doziranje, produženo oslobađanje lekovite supstance, hemijsku stabilnost i odsustvo
konzervansa. Iako je uložen značajan napor u okviru razvoja oftalmoloških terapijskih
sistema, ključni izazov je još njihova primena u kliničkoj praksi (2). Osnovni zahtev koji ovi
sistemi moraju ispuniti odnosi se na biokompatibilnost, jer njihove komponente ne bi trebalo
da stupaju u interakciju sa okolnim tkivom ili da izazovu reakcije stranog tela putem
inflamatornog ili imunološkog odgovora (1). Konačno, uprkos brojnim izazovima, dalja
istraživanja usmerena ka razvoju inovativne primene polimernih materijala za oftalmološku
primenu očekuju se u bliskoj budućnosti.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Polymers in ophthalmic preparations: from traditional application to product innovation, Polimeri u oftalmološkim preparatima: od tradicionalne primene do inovacije proizvoda",
volume = "72",
number = "4 suplement",
pages = "113-114",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4462"
}

Krajišnik, D.. (2022). Polymers in ophthalmic preparations: from traditional application to product innovation. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), 113-114.
https://hdl.handle.net/21.15107/rcub_farfar_4462

Krajišnik D. Polymers in ophthalmic preparations: from traditional application to product innovation. in Arhiv za farmaciju. 2022;72(4 suplement):113-114.
https://hdl.handle.net/21.15107/rcub_farfar_4462 .

Krajišnik, Danina, "Polymers in ophthalmic preparations: from traditional application to product innovation" in Arhiv za farmaciju, 72, no. 4 suplement (2022):113-114,
https://hdl.handle.net/21.15107/rcub_farfar_4462 .

TY  - JOUR
AU  - Račić, Andjelka
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Jurišić Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Micov, Ana
AU  - Vuković, Milja
AU  - Stepanović-Petrović, Radica
AU  - Krajišnik, Danina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3938
AB  - The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.
PB  - Elsevier B.V.
T2  - European Journal of Pharmaceutical Sciences
T1  - Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches
VL  - 166
DO  - 10.1016/j.ejps.2021.105906
ER  - 

@article{
author = "Račić, Andjelka and Čalija, Bojan and Milić, Jela and Jurišić Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Micov, Ana and Vuković, Milja and Stepanović-Petrović, Radica and Krajišnik, Danina",
year = "2021",
abstract = "The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.",
publisher = "Elsevier B.V.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches",
volume = "166",
doi = "10.1016/j.ejps.2021.105906"
}

Račić, A., Čalija, B., Milić, J., Jurišić Dukovski, B., Lovrić, J., Dobričić, V., Micov, A., Vuković, M., Stepanović-Petrović, R.,& Krajišnik, D.. (2021). Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches. in European Journal of Pharmaceutical Sciences
Elsevier B.V.., 166.
https://doi.org/10.1016/j.ejps.2021.105906

Račić A, Čalija B, Milić J, Jurišić Dukovski B, Lovrić J, Dobričić V, Micov A, Vuković M, Stepanović-Petrović R, Krajišnik D. Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches. in European Journal of Pharmaceutical Sciences. 2021;166.
doi:10.1016/j.ejps.2021.105906 .

Račić, Andjelka, Čalija, Bojan, Milić, Jela, Jurišić Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Micov, Ana, Vuković, Milja, Stepanović-Petrović, Radica, Krajišnik, Danina, "Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches" in European Journal of Pharmaceutical Sciences, 166 (2021),
https://doi.org/10.1016/j.ejps.2021.105906 . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Jurišić Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5481
AB  - Olopatadine is a selective H1-receptore antagonist that
inhibits release of histamine and proinflammatory
mediators [1]. The poor ocular bioavailability, as the main
limitation of efficient ocular therapy, could be overcome by
increasing residence time and enhancing corneal
penetration. ...
PB  - International Association for Pharmaceutical Technology, Mainz, Germany
C3  - 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting
T1  - Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5481
ER  - 

@conference{
author = "Račić, Anđelka and Jurišić Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2021",
abstract = "Olopatadine is a selective H1-receptore antagonist that
inhibits release of histamine and proinflammatory
mediators [1]. The poor ocular bioavailability, as the main
limitation of efficient ocular therapy, could be overcome by
increasing residence time and enhancing corneal
penetration. ...",
publisher = "International Association for Pharmaceutical Technology, Mainz, Germany",
journal = "12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting",
title = "Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5481"
}

Račić, A., Jurišić Dukovski, B., Lovrić, J., Dobričić, V., Čalija, B., Milić, J.,& Krajišnik, D.. (2021). Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model. in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting
International Association for Pharmaceutical Technology, Mainz, Germany..
https://hdl.handle.net/21.15107/rcub_farfar_5481

Račić A, Jurišić Dukovski B, Lovrić J, Dobričić V, Čalija B, Milić J, Krajišnik D. Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model. in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5481 .

Račić, Anđelka, Jurišić Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model" in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5481 .

TY  - JOUR
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Daković, Aleksandra
AU  - Damjanović, Ana
AU  - Krstić, Jugoslav
AU  - Stojanović, Jovica
AU  - Čalija, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3796
AB  - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
VL  - 123
DO  - 10.1016/j.msec.2021.112029
ER  - 

@article{
author = "Jauković, Valentina and Krajišnik, Danina and Daković, Aleksandra and Damjanović, Ana and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan",
year = "2021",
abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release",
volume = "123",
doi = "10.1016/j.msec.2021.112029"
}

Jauković, V., Krajišnik, D., Daković, A., Damjanović, A., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C
Elsevier Ltd., 123.
https://doi.org/10.1016/j.msec.2021.112029

Jauković V, Krajišnik D, Daković A, Damjanović A, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123.
doi:10.1016/j.msec.2021.112029 .

Jauković, Valentina, Krajišnik, Danina, Daković, Aleksandra, Damjanović, Ana, Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021),
https://doi.org/10.1016/j.msec.2021.112029 . .

TY  - JOUR
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3947
AB  - We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.
PB  - Springer Science and Business Media B.V.
T2  - Journal of Nanoparticle Research
T1  - Safe-by-design gelatin-modified zinc oxide nanoparticles
VL  - 23
IS  - 9
DO  - 10.1007/s11051-021-05312-3
ER  - 

@article{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Đekić, Ljiljana and Krajišnik, Danina and Filipič, Metka and Stevanović, Magdalena",
year = "2021",
abstract = "We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.",
publisher = "Springer Science and Business Media B.V.",
journal = "Journal of Nanoparticle Research",
title = "Safe-by-design gelatin-modified zinc oxide nanoparticles",
volume = "23",
number = "9",
doi = "10.1007/s11051-021-05312-3"
}

Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Đekić, L., Krajišnik, D., Filipič, M.,& Stevanović, M.. (2021). Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research
Springer Science and Business Media B.V.., 23(9).
https://doi.org/10.1007/s11051-021-05312-3

Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Đekić L, Krajišnik D, Filipič M, Stevanović M. Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research. 2021;23(9).
doi:10.1007/s11051-021-05312-3 .

Janićijević, Željko, Stanković, Ana, Žegura, Bojana, Veljović, Đorđe, Đekić, Ljiljana, Krajišnik, Danina, Filipič, Metka, Stevanović, Magdalena, "Safe-by-design gelatin-modified zinc oxide nanoparticles" in Journal of Nanoparticle Research, 23, no. 9 (2021),
https://doi.org/10.1007/s11051-021-05312-3 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3875
AB  - Phytosomes  are  amphiphilic  molecular  complexes  of  substances  of  plant  origin  and phospholipids that are considered as active ingredients of dermopharmaceutical and cosmetic formulations of potentially improved efficiency. The study aim was the formulation of carbomer hydrogels with commercially available phytosomes of escin (Escin ß-Sitosterol Phytosome®) (EP) and 18-ß glycyrrhetinic acid (18-ß Glycyrrhetinic Acid Phytosome®) (GP) and evaluation of their application properties and real-time physical stability. Phytosomes incorporation did not significantly  affect  pH  of  the  hydrogels,  which  was  acceptable for  cutaneous  application. However, these hydrogels had significantly different organoleptic properties (opaque and softer consistency) compared to the hydrogel without active substance (C) and the hydrogels with pure active substances (E and G) used for comparison. The values of maximum and minimum apparent viscosity and yield stress were significantly lower in phytosome-loaded hydrogels. The results of oscillatory rheological analysis indicated that viscous character prevails in EP and GP hydrogels (elastic modulus (G')˂viscous modulus (G")), while in hydrogels C, E and G elastic properties were more pronounced (G'˃G"). Escin phytosome had greater influence on carbomer gel network strength. Phytosome-loaded hydrogels were physically stable during 24 months of storage under ambient  conditions,  although  the  rheological  analysis  also  indicated  a  potential  risk  of sedimentation.
AB  - Fitosomi su amfifilni molekulski kompleksi supstanci biljnog porekla i fosfolipida koji se razmatraju kao aktivni sastojci dermofarmaceutskih i kozmetičkih formulacija potencijalno unapređene efikasnosti. Cilj studije bio je formulacija karbomernih hidrogelova sa komercijalno dostupnim fitosomima escina (Escin ß-Sitosterol Phytosome®) (EP) i 18-ß gliciretinske kiseline (18-ß Glycyrrhetinic Acid Phytosome®) (GP) i njihova karakterizacija u cilju procene aplikativnih svojstava i fizičke stabilnosti u realnom vremenu. Inkorporiranje fitosoma nije značajno uticalo na pH hidrogelova, koji je bio prihvatljiv za primenu na koži. Međutim, ovi hidrogelovi imali su značajno različite organoleptičke osobine (neprozirni i mekše konzistencije) u poređenju sa hidrogelom bez aktivne supstance (C) i odgovarajućim hidrogelovima sa čistim aktivnim supstancama (E i G) koji su upotrebljeni za poređenje. Vrednosti maksimalnog i minimalnog prividnog viskoziteta i napon popuštanja bili su značajno niži kod hidrogelova sa fitosomima. Rezultati oscilatorne reološke analize ukazali su da kod hidrogelova EP i GP preovlađuje viskozni karakter (elastični modul (G')˂viskozni modul (G")), dok su kod hidrogelova C, E i G bila izraženija elastična svojstva (G'˃G"). Fitosom escina je imao veći uticaj na jačinu karbomerne gelske mreže. Hidrogelovi sa fitosomima bili su fizički stabilni tokom 24 meseca čuvanja pod ambijentalnim uslovima, mada su rezultati reološke analize ukazali na potencijalni rizik od sedimentacije.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Rheological behavior study and its significance in the assessment of application properties and physical stability of phytosome loaded hydrogels
T1  - Ispitivanje reološkog ponašanja i njegov značaj za procenu aplikativnih svojstava i fizičke stabilnosti hidrogelova sa fitosomima
VL  - 71
IS  - 2
SP  - 120
EP  - 140
DO  - 10.5937/arhfarm71-30708
ER  - 

@article{
author = "Đekić, Ljiljana and Krajišnik, Danina",
year = "2021",
abstract = "Phytosomes  are  amphiphilic  molecular  complexes  of  substances  of  plant  origin  and phospholipids that are considered as active ingredients of dermopharmaceutical and cosmetic formulations of potentially improved efficiency. The study aim was the formulation of carbomer hydrogels with commercially available phytosomes of escin (Escin ß-Sitosterol Phytosome®) (EP) and 18-ß glycyrrhetinic acid (18-ß Glycyrrhetinic Acid Phytosome®) (GP) and evaluation of their application properties and real-time physical stability. Phytosomes incorporation did not significantly  affect  pH  of  the  hydrogels,  which  was  acceptable for  cutaneous  application. However, these hydrogels had significantly different organoleptic properties (opaque and softer consistency) compared to the hydrogel without active substance (C) and the hydrogels with pure active substances (E and G) used for comparison. The values of maximum and minimum apparent viscosity and yield stress were significantly lower in phytosome-loaded hydrogels. The results of oscillatory rheological analysis indicated that viscous character prevails in EP and GP hydrogels (elastic modulus (G')˂viscous modulus (G")), while in hydrogels C, E and G elastic properties were more pronounced (G'˃G"). Escin phytosome had greater influence on carbomer gel network strength. Phytosome-loaded hydrogels were physically stable during 24 months of storage under ambient  conditions,  although  the  rheological  analysis  also  indicated  a  potential  risk  of sedimentation., Fitosomi su amfifilni molekulski kompleksi supstanci biljnog porekla i fosfolipida koji se razmatraju kao aktivni sastojci dermofarmaceutskih i kozmetičkih formulacija potencijalno unapređene efikasnosti. Cilj studije bio je formulacija karbomernih hidrogelova sa komercijalno dostupnim fitosomima escina (Escin ß-Sitosterol Phytosome®) (EP) i 18-ß gliciretinske kiseline (18-ß Glycyrrhetinic Acid Phytosome®) (GP) i njihova karakterizacija u cilju procene aplikativnih svojstava i fizičke stabilnosti u realnom vremenu. Inkorporiranje fitosoma nije značajno uticalo na pH hidrogelova, koji je bio prihvatljiv za primenu na koži. Međutim, ovi hidrogelovi imali su značajno različite organoleptičke osobine (neprozirni i mekše konzistencije) u poređenju sa hidrogelom bez aktivne supstance (C) i odgovarajućim hidrogelovima sa čistim aktivnim supstancama (E i G) koji su upotrebljeni za poređenje. Vrednosti maksimalnog i minimalnog prividnog viskoziteta i napon popuštanja bili su značajno niži kod hidrogelova sa fitosomima. Rezultati oscilatorne reološke analize ukazali su da kod hidrogelova EP i GP preovlađuje viskozni karakter (elastični modul (G')˂viskozni modul (G")), dok su kod hidrogelova C, E i G bila izraženija elastična svojstva (G'˃G"). Fitosom escina je imao veći uticaj na jačinu karbomerne gelske mreže. Hidrogelovi sa fitosomima bili su fizički stabilni tokom 24 meseca čuvanja pod ambijentalnim uslovima, mada su rezultati reološke analize ukazali na potencijalni rizik od sedimentacije.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Rheological behavior study and its significance in the assessment of application properties and physical stability of phytosome loaded hydrogels, Ispitivanje reološkog ponašanja i njegov značaj za procenu aplikativnih svojstava i fizičke stabilnosti hidrogelova sa fitosomima",
volume = "71",
number = "2",
pages = "120-140",
doi = "10.5937/arhfarm71-30708"
}

Đekić, L.,& Krajišnik, D.. (2021). Rheological behavior study and its significance in the assessment of application properties and physical stability of phytosome loaded hydrogels. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(2), 120-140.
https://doi.org/10.5937/arhfarm71-30708

Đekić L, Krajišnik D. Rheological behavior study and its significance in the assessment of application properties and physical stability of phytosome loaded hydrogels. in Arhiv za farmaciju. 2021;71(2):120-140.
doi:10.5937/arhfarm71-30708 .

Đekić, Ljiljana, Krajišnik, Danina, "Rheological behavior study and its significance in the assessment of application properties and physical stability of phytosome loaded hydrogels" in Arhiv za farmaciju, 71, no. 2 (2021):120-140,
https://doi.org/10.5937/arhfarm71-30708 . .

TY  - JOUR
AU  - Spasojević, Milica
AU  - Daković, Aleksandra
AU  - Rottinghaus, George E.
AU  - Obradović, Milena
AU  - Krajišnik, Danina
AU  - Marković, Marija
AU  - Krstić, Jugoslav
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3795
AB  - A natural kaolin from Serbia was modified with different amounts of octadecyldimethylbenzyl ammonium (ODMBA) - (25, 50 and 90% of kaolin cation exchange capacity). Samples were denoted as OKR-25, OKR-50 and OKR-90. Several methods (FTIR spectroscopy, thermal analysis, zeta potential measurements, and N2 physisorption) were used for characterization of the organokaolinites. Adsorption of the common mycotoxins - ochratoxin A (OCHRA) and zearalenone (ZEN) by the organokaolinites was investigated at different levels of solid phase in suspension, different initial mycotoxin concentrations and different pH values. The natural kaolin was not effective in binding OCHRA or ZEN. Adsorption of both mycotoxins by organokaolinites increased with increasing amounts of solid phase as well as with increasing levels of surfactant on the kaolin surface. OCHRA and ZEN adsorption by all organokaolinites followed non-linear adsorption isotherms, at pH 3, 7 and 9. The maximum adsorption capacity for OCHRA adsorption was at pH 3 (4.8 mg/g for OKR-25, 26.7 mg/g for OKR-50 and 39.2 mg/g for OKR-90) that was calculated from the Langmuir model. Much lower OCHRA adsorption capacities were found at pH 7 and 9 (from 0.8 mg/g to 6.9 mg/g at pH 7 and from 1.1 mg/g to 4.6 mg/g at pH 9). The following adsorption capacities for ZEN were obtained from the Langmuir isotherms, at pH 3: 4.5 mg/g for OKR-25, 12.0 mg/g for OKR-50 and 13.5 mg/g for OKR-90. At pH 7, adsorption of ZEN was 5.7 mg/g for OKR-25, 15.3 mg/g for OKR-90 and 14. 4 mg/g for OKR-90. At pH 9, ZEN adsorption capacities were 2.4, 14.1 and 8.1 mg/g for OKR-25, OKR-50 and OKR-90, respectively. Thus, at the lowest amount of ODMBA at the kaolin surface, adsorption of ZEN was similar at pH 3 and 7, while a slightly lower value was obtained for its adsorption at pH 9. With increasing amounts of organic phase at the kaolin surface, the adsorption of ZEN was practically independent of pH. Adsorption of both mycotoxins was dependent on the amount of ODMBA ions at the kaolin surface as well as on their forms in solution.
PB  - Elsevier Ltd
T2  - Applied Clay Science
T1  - Influence of surface coverage of kaolin with surfactant ions on adsorption of ochratoxin A and zearalenone
VL  - 205
DO  - 10.1016/j.clay.2021.106040
ER  - 

@article{
author = "Spasojević, Milica and Daković, Aleksandra and Rottinghaus, George E. and Obradović, Milena and Krajišnik, Danina and Marković, Marija and Krstić, Jugoslav",
year = "2021",
abstract = "A natural kaolin from Serbia was modified with different amounts of octadecyldimethylbenzyl ammonium (ODMBA) - (25, 50 and 90% of kaolin cation exchange capacity). Samples were denoted as OKR-25, OKR-50 and OKR-90. Several methods (FTIR spectroscopy, thermal analysis, zeta potential measurements, and N2 physisorption) were used for characterization of the organokaolinites. Adsorption of the common mycotoxins - ochratoxin A (OCHRA) and zearalenone (ZEN) by the organokaolinites was investigated at different levels of solid phase in suspension, different initial mycotoxin concentrations and different pH values. The natural kaolin was not effective in binding OCHRA or ZEN. Adsorption of both mycotoxins by organokaolinites increased with increasing amounts of solid phase as well as with increasing levels of surfactant on the kaolin surface. OCHRA and ZEN adsorption by all organokaolinites followed non-linear adsorption isotherms, at pH 3, 7 and 9. The maximum adsorption capacity for OCHRA adsorption was at pH 3 (4.8 mg/g for OKR-25, 26.7 mg/g for OKR-50 and 39.2 mg/g for OKR-90) that was calculated from the Langmuir model. Much lower OCHRA adsorption capacities were found at pH 7 and 9 (from 0.8 mg/g to 6.9 mg/g at pH 7 and from 1.1 mg/g to 4.6 mg/g at pH 9). The following adsorption capacities for ZEN were obtained from the Langmuir isotherms, at pH 3: 4.5 mg/g for OKR-25, 12.0 mg/g for OKR-50 and 13.5 mg/g for OKR-90. At pH 7, adsorption of ZEN was 5.7 mg/g for OKR-25, 15.3 mg/g for OKR-90 and 14. 4 mg/g for OKR-90. At pH 9, ZEN adsorption capacities were 2.4, 14.1 and 8.1 mg/g for OKR-25, OKR-50 and OKR-90, respectively. Thus, at the lowest amount of ODMBA at the kaolin surface, adsorption of ZEN was similar at pH 3 and 7, while a slightly lower value was obtained for its adsorption at pH 9. With increasing amounts of organic phase at the kaolin surface, the adsorption of ZEN was practically independent of pH. Adsorption of both mycotoxins was dependent on the amount of ODMBA ions at the kaolin surface as well as on their forms in solution.",
publisher = "Elsevier Ltd",
journal = "Applied Clay Science",
title = "Influence of surface coverage of kaolin with surfactant ions on adsorption of ochratoxin A and zearalenone",
volume = "205",
doi = "10.1016/j.clay.2021.106040"
}

Spasojević, M., Daković, A., Rottinghaus, G. E., Obradović, M., Krajišnik, D., Marković, M.,& Krstić, J.. (2021). Influence of surface coverage of kaolin with surfactant ions on adsorption of ochratoxin A and zearalenone. in Applied Clay Science
Elsevier Ltd., 205.
https://doi.org/10.1016/j.clay.2021.106040

Spasojević M, Daković A, Rottinghaus GE, Obradović M, Krajišnik D, Marković M, Krstić J. Influence of surface coverage of kaolin with surfactant ions on adsorption of ochratoxin A and zearalenone. in Applied Clay Science. 2021;205.
doi:10.1016/j.clay.2021.106040 .

Spasojević, Milica, Daković, Aleksandra, Rottinghaus, George E., Obradović, Milena, Krajišnik, Danina, Marković, Marija, Krstić, Jugoslav, "Influence of surface coverage of kaolin with surfactant ions on adsorption of ochratoxin A and zearalenone" in Applied Clay Science, 205 (2021),
https://doi.org/10.1016/j.clay.2021.106040 . .

TY  - JOUR
AU  - Lemoine, Céline
AU  - Thakur, Aneesh
AU  - Krajišnik, Danina
AU  - Guyon, Romain
AU  - Longet, S.
AU  - Razim, Agnieszka
AU  - Górska, Sabina
AU  - Pantelić, Ivana
AU  - Ilić, Tanja
AU  - Nikolić, Ines
AU  - Lavelle, Ed C.
AU  - Gamian, Ed C.
AU  - Savić, Snežana
AU  - Milicic, Anita
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3627
AB  - Vaccination has been well recognised as a critically important tool in preventing infectious disease, yet incomplete immunisation coverage remains a major obstacle to achieving disease control and eradication. As medical products for global access, vaccines need to be safe, effective and inexpensive. In line with these goals, continuous improvements of vaccine delivery strategies are necessary to achieve the full potential of immunisation. Novel technologies related to vaccine delivery and route of administration, use of advanced adjuvants and controlled antigen release (single-dose immunisation) approaches are expected to contribute to improved coverage and patient compliance. This review discusses the application of micro-and nano-technologies in the alternative routes of vaccine administration (mucosal and cutaneous vaccination), oral vaccine delivery as well as vaccine encapsulation with the aim of controlled antigen release for single-dose vaccination.
PB  - MDPI AG
T2  - Vaccines
T1  - Technological approaches for improving vaccination compliance and coverage
VL  - 8
IS  - 2
SP  - 1
EP  - 28
DO  - 10.3390/vaccines8020304
ER  - 

@article{
author = "Lemoine, Céline and Thakur, Aneesh and Krajišnik, Danina and Guyon, Romain and Longet, S. and Razim, Agnieszka and Górska, Sabina and Pantelić, Ivana and Ilić, Tanja and Nikolić, Ines and Lavelle, Ed C. and Gamian, Ed C. and Savić, Snežana and Milicic, Anita",
year = "2020",
abstract = "Vaccination has been well recognised as a critically important tool in preventing infectious disease, yet incomplete immunisation coverage remains a major obstacle to achieving disease control and eradication. As medical products for global access, vaccines need to be safe, effective and inexpensive. In line with these goals, continuous improvements of vaccine delivery strategies are necessary to achieve the full potential of immunisation. Novel technologies related to vaccine delivery and route of administration, use of advanced adjuvants and controlled antigen release (single-dose immunisation) approaches are expected to contribute to improved coverage and patient compliance. This review discusses the application of micro-and nano-technologies in the alternative routes of vaccine administration (mucosal and cutaneous vaccination), oral vaccine delivery as well as vaccine encapsulation with the aim of controlled antigen release for single-dose vaccination.",
publisher = "MDPI AG",
journal = "Vaccines",
title = "Technological approaches for improving vaccination compliance and coverage",
volume = "8",
number = "2",
pages = "1-28",
doi = "10.3390/vaccines8020304"
}

Lemoine, C., Thakur, A., Krajišnik, D., Guyon, R., Longet, S., Razim, A., Górska, S., Pantelić, I., Ilić, T., Nikolić, I., Lavelle, E. C., Gamian, E. C., Savić, S.,& Milicic, A.. (2020). Technological approaches for improving vaccination compliance and coverage. in Vaccines
MDPI AG., 8(2), 1-28.
https://doi.org/10.3390/vaccines8020304

Lemoine C, Thakur A, Krajišnik D, Guyon R, Longet S, Razim A, Górska S, Pantelić I, Ilić T, Nikolić I, Lavelle EC, Gamian EC, Savić S, Milicic A. Technological approaches for improving vaccination compliance and coverage. in Vaccines. 2020;8(2):1-28.
doi:10.3390/vaccines8020304 .

Lemoine, Céline, Thakur, Aneesh, Krajišnik, Danina, Guyon, Romain, Longet, S., Razim, Agnieszka, Górska, Sabina, Pantelić, Ivana, Ilić, Tanja, Nikolić, Ines, Lavelle, Ed C., Gamian, Ed C., Savić, Snežana, Milicic, Anita, "Technological approaches for improving vaccination compliance and coverage" in Vaccines, 8, no. 2 (2020):1-28,
https://doi.org/10.3390/vaccines8020304 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Krajišnik, Danina
AU  - Čalija, Bojan
AU  - Đekić, Ljiljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3654
AB  - The formulation of biocompatible drug carriers based on cationic biopolymer chitosan and natural or synthetic polymers represents an important research interest. Therefore, this review aims to perceive their potential in drug delivery. The most investigated chitosan-based polymer blends are polyelectrolyte complexes (PECs) obtained by establishing ionic interactions with biocompatible   polyanions   as   alginates,   pectin,   xanthan   gum,   carrageenan, carboxymethylcellulose, and collagen. Depending on the preparation conditions, PECs could be prepared  in  versatile  forms  including  membranes/films,  hydrogel  beads,  nanoparticles,  and microparticles, to achieve controlled (e.g., extended, delayed, colon-specific and pH-dependent) drug delivery. PECs can encapsulate hydrophilic and lipophilic drug substances with different molecular  weights.  Drug  encapsulation  allows  the  preservation  of  their  structure,  activity, improvement in absorption efficiency, reduction in adverse effects and long-term stability in vitroand in  vivo. The biocompatible structures as non-covalent chitosan-based complexes could be formed also by establishing hydrogen bonds, for example with poly(vinyl alcohol). The swelling of these complexes is not pH-dependent and encapsulated drug substances are often released by already known types of diffusion. Moreover, grafted chitosan derivatives (e.g., carboxymethyl chitosan, trimethyl chitosan, acrylated chitosan) are synthesized to improve water solubility at a wide pH range and enhance the encapsulation capacity of promising PEC-based drug carriers.
AB  - Formulacija biokompatibilnih nosača lekovitih supstanci na bazi katjonskog biopolimera hitozana i prirodnih ili sintetskih polimera predstavlja značajan istraživački interes. Stoga je cilj ovog rada sagledati njihovu potencijalnu primenu kao nosača lekovitih supstanci. Najistraženije blende polimera na bazi hitozana su polielektrolitni kompleksi (PEK) dobijeni uspostavljanjem jonskih interakcija sa biokompatibilnim polianjonima, npr. alginatom, pektinom, ksantan gumom, karagenanom, karboksimetilcelulozom i kolagenom. U zavisnosti od uslova pripreme, mogu se formulisati PEK u vidu membrana/filmova, hidrogelnih perli, nanočestica, mikročestica ili drugih tipova nosača, sa ciljem postizanja kontrolisanog (npr. produženog, odloženog, kolon-specifičnog i pH-zavisnog) oslobađanja lekovitih supstanci. PEK su pogodni za inkapsulaciju hidrofilnih ili lipofilnih lekovitih supstanci različitih molekulskih masa. Inkapsulacija obezbeđuje očuvanje njihove strukture, aktivnosti, poboljšanje apsorpcije, smanjenje štetnih efekata i dugoročnu stabilnost in vitro i in vivo. Biokompatibilne strukture nalik kompleksima na bazi hitozana mogu se formirati i uspostavljanjem vodoničnih veza, kao što je slučaj sa polivinil alkoholom. Njihovo bubrenje ne zavisi od pH. Inkapsulirane lekovite supstance se najčešće oslobađaju prema nekom od već poznatih tipova difuzije. Dodatno, različiti derivati hitozana (npr. karboksimetilhitozan, trimetilhitozan, akril derivati hitozana) sintetisani su radi poboljšanja rastvorljivosti polimera u vodi u širokom opsegu pH i povećanja kapaciteta za inkapsulaciju lekovitih supstanci tako dobijenih PEK, koji takođe predstavljaju obećavajuće nosače.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery
T1  - Biokompatibilni nekovalentni kompleksi hitozana sa različitim polimerima - svojstva i primena kao nosača lekovitih supstanci
VL  - 70
IS  - 4
SP  - 173
EP  - 197
DO  - 10.5937/arhfarm2004173Q
ER  - 

@article{
author = "Ćirić, Ana and Krajišnik, Danina and Čalija, Bojan and Đekić, Ljiljana",
year = "2020",
abstract = "The formulation of biocompatible drug carriers based on cationic biopolymer chitosan and natural or synthetic polymers represents an important research interest. Therefore, this review aims to perceive their potential in drug delivery. The most investigated chitosan-based polymer blends are polyelectrolyte complexes (PECs) obtained by establishing ionic interactions with biocompatible   polyanions   as   alginates,   pectin,   xanthan   gum,   carrageenan, carboxymethylcellulose, and collagen. Depending on the preparation conditions, PECs could be prepared  in  versatile  forms  including  membranes/films,  hydrogel  beads,  nanoparticles,  and microparticles, to achieve controlled (e.g., extended, delayed, colon-specific and pH-dependent) drug delivery. PECs can encapsulate hydrophilic and lipophilic drug substances with different molecular  weights.  Drug  encapsulation  allows  the  preservation  of  their  structure,  activity, improvement in absorption efficiency, reduction in adverse effects and long-term stability in vitroand in  vivo. The biocompatible structures as non-covalent chitosan-based complexes could be formed also by establishing hydrogen bonds, for example with poly(vinyl alcohol). The swelling of these complexes is not pH-dependent and encapsulated drug substances are often released by already known types of diffusion. Moreover, grafted chitosan derivatives (e.g., carboxymethyl chitosan, trimethyl chitosan, acrylated chitosan) are synthesized to improve water solubility at a wide pH range and enhance the encapsulation capacity of promising PEC-based drug carriers., Formulacija biokompatibilnih nosača lekovitih supstanci na bazi katjonskog biopolimera hitozana i prirodnih ili sintetskih polimera predstavlja značajan istraživački interes. Stoga je cilj ovog rada sagledati njihovu potencijalnu primenu kao nosača lekovitih supstanci. Najistraženije blende polimera na bazi hitozana su polielektrolitni kompleksi (PEK) dobijeni uspostavljanjem jonskih interakcija sa biokompatibilnim polianjonima, npr. alginatom, pektinom, ksantan gumom, karagenanom, karboksimetilcelulozom i kolagenom. U zavisnosti od uslova pripreme, mogu se formulisati PEK u vidu membrana/filmova, hidrogelnih perli, nanočestica, mikročestica ili drugih tipova nosača, sa ciljem postizanja kontrolisanog (npr. produženog, odloženog, kolon-specifičnog i pH-zavisnog) oslobađanja lekovitih supstanci. PEK su pogodni za inkapsulaciju hidrofilnih ili lipofilnih lekovitih supstanci različitih molekulskih masa. Inkapsulacija obezbeđuje očuvanje njihove strukture, aktivnosti, poboljšanje apsorpcije, smanjenje štetnih efekata i dugoročnu stabilnost in vitro i in vivo. Biokompatibilne strukture nalik kompleksima na bazi hitozana mogu se formirati i uspostavljanjem vodoničnih veza, kao što je slučaj sa polivinil alkoholom. Njihovo bubrenje ne zavisi od pH. Inkapsulirane lekovite supstance se najčešće oslobađaju prema nekom od već poznatih tipova difuzije. Dodatno, različiti derivati hitozana (npr. karboksimetilhitozan, trimetilhitozan, akril derivati hitozana) sintetisani su radi poboljšanja rastvorljivosti polimera u vodi u širokom opsegu pH i povećanja kapaciteta za inkapsulaciju lekovitih supstanci tako dobijenih PEK, koji takođe predstavljaju obećavajuće nosače.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery, Biokompatibilni nekovalentni kompleksi hitozana sa različitim polimerima - svojstva i primena kao nosača lekovitih supstanci",
volume = "70",
number = "4",
pages = "173-197",
doi = "10.5937/arhfarm2004173Q"
}

Ćirić, A., Krajišnik, D., Čalija, B.,& Đekić, L.. (2020). Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 70(4), 173-197.
https://doi.org/10.5937/arhfarm2004173Q

Ćirić A, Krajišnik D, Čalija B, Đekić L. Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery. in Arhiv za farmaciju. 2020;70(4):173-197.
doi:10.5937/arhfarm2004173Q .

Ćirić, Ana, Krajišnik, Danina, Čalija, Bojan, Đekić, Ljiljana, "Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery" in Arhiv za farmaciju, 70, no. 4 (2020):173-197,
https://doi.org/10.5937/arhfarm2004173Q . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milašinović, Nikola
AU  - Daković, Aleksandra
AU  - Trifković, Kata
AU  - Stojanović, Jovica
AU  - Krajišnik, Danina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3476
AB  - This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.
PB  - Wiley Periodicals, Inc.
T2  - Journal of Applied Polymer Science
T1  - Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
VL  - 137
IS  - 8
DO  - 10.1002/app.48406
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Milašinović, Nikola and Daković, Aleksandra and Trifković, Kata and Stojanović, Jovica and Krajišnik, Danina",
year = "2020",
abstract = "This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.",
publisher = "Wiley Periodicals, Inc.",
journal = "Journal of Applied Polymer Science",
title = "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass",
volume = "137",
number = "8",
doi = "10.1002/app.48406"
}

Čalija, B., Milić, J., Milašinović, N., Daković, A., Trifković, K., Stojanović, J.,& Krajišnik, D.. (2020). Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science
Wiley Periodicals, Inc.., 137(8).
https://doi.org/10.1002/app.48406

Čalija B, Milić J, Milašinović N, Daković A, Trifković K, Stojanović J, Krajišnik D. Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science. 2020;137(8).
doi:10.1002/app.48406 .

Čalija, Bojan, Milić, Jela, Milašinović, Nikola, Daković, Aleksandra, Trifković, Kata, Stojanović, Jovica, Krajišnik, Danina, "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass" in Journal of Applied Polymer Science, 137, no. 8 (2020),
https://doi.org/10.1002/app.48406 . .

TY  - JOUR
AU  - Krajišnik, Danina
AU  - Ilić, Tanja
AU  - Nikolić, Ines
AU  - Savić, Snežana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3567
AB  - In the era of modern vaccinology, limited immunogenicity of the most commonly used antigens has enforced the use of various adjuvants in vaccine formulations to achieve desired immune  response.  Aluminum-containing  adjuvants  have  been,  historically,  the  most  widely used mineral immunostimulants, generally regarded as safe to use in human vaccines. The great academic   progress   in   inorganic   (nano)materials   synthesis,   structure   control   and functionalization design has  led  to  a  growing  interest  in innovative  adjuvants  such  as  clays, mesoporous silica nanoparticles, zinc oxide, iron oxide and iron hydroxide nanoparticles, etc. On the other hand, there has been an intention to use specific nanoparticulated antigen delivery systems,  such  as  nanoemulsions,  in  order  to  protect  antigens  from  premature  proteolytic degradation  and/or  to  improve  antigen  immunogenicity  by  facilitating  antigen  uptake  and processing by antigen presenting cells. Simultaneously, numerous research efforts have been focused on the development of innovative technologies for antigen delivery into the skin (such as microneedles), with the aim to improve vaccine efficacy alongside with enhanced patient adherence,  particularly  in  children  population  (noninvasive  or minimally  invasive administration). Therefore, this review deals with each of these approaches in more detail, with the special emphasis on examples of their use in vaccine formulations as well as on the factors influencing their efficacy and safety.
AB  - U  doba  savremene  vakcinologije,  ograničena  imunogenost  većine  korišćenih  antigena podstakla  je  primenu  različitih  adjuvanasa  u  formulacijama  vakcina  radi  postizanja  željenog imunskog odgovora. Mineralni adjuvansi na bazi aluminijuma su istorijski najčešće korišćeni imunostimulansi u vakcinama i smatraju se generalno bezbednim za humanu upotrebu. Značajni napredak  na  polju  sinteze,  kontrole  strukture  i  funkcionalnog  dizajna  neorganskih (nano)materijala uslovio je povećano interesovanje za primenom inovativnih adjuvanasa kao što su  gline,  mezoporozne  silika  nanočestice,  nanočestice  cink-oksida,  gvožđe  oksida  i  gvožđe hidroksida, i dr. Sa druge strane, uočava se i sve veće interesovanje za primenom specifičnih nanonosača  antigena,  kao  što  su  nanoemulzije,  kako  bi  se  antigeni  zaštitili  od  preuranjene proteolitičke  degradacije  i/ili poboljšala  njihova  imunogenost,  olakšavanjem  preuzimanja  i obrade  od  strane  antigen-prezentujućih  ćelija.  Takođe,  brojni  istraživački  napori  tokom poslednjih nekoliko godina usmereni su ka razvoju inovativnih tehnologija za isporuku antigena u kožu (kao što su mikroigle) radi poboljšanja efikasnosti vakcinacije uz istovremeno povećanje adherence pacijenata, posebno u pedijatrijskoj populaciji (neinvazivan ili minimalno invazivan način primene). Otuda, u ovom preglednom radu dat je detaljan pregled karakteristika svakog od  navedenih  pristupa  za  poboljšanje  efikasnosti  vakcina,  sa  posebnim  osvrtom  na  primere njihove primene u formulacijama vakcina i faktore koji određuju efikasnost i bezbednost.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems
T1  - Konvencionalni i napredni adjuvansi u formulacijama vakcina: mineralni adsorbenti, nanočestični nosači i sistemi tipa mikroigala
VL  - 69
IS  - 6
SP  - 420
EP  - 451
DO  - 10.5937/arhfarm1906420K
ER  - 

@article{
author = "Krajišnik, Danina and Ilić, Tanja and Nikolić, Ines and Savić, Snežana",
year = "2019",
abstract = "In the era of modern vaccinology, limited immunogenicity of the most commonly used antigens has enforced the use of various adjuvants in vaccine formulations to achieve desired immune  response.  Aluminum-containing  adjuvants  have  been,  historically,  the  most  widely used mineral immunostimulants, generally regarded as safe to use in human vaccines. The great academic   progress   in   inorganic   (nano)materials   synthesis,   structure   control   and functionalization design has  led  to  a  growing  interest  in innovative  adjuvants  such  as  clays, mesoporous silica nanoparticles, zinc oxide, iron oxide and iron hydroxide nanoparticles, etc. On the other hand, there has been an intention to use specific nanoparticulated antigen delivery systems,  such  as  nanoemulsions,  in  order  to  protect  antigens  from  premature  proteolytic degradation  and/or  to  improve  antigen  immunogenicity  by  facilitating  antigen  uptake  and processing by antigen presenting cells. Simultaneously, numerous research efforts have been focused on the development of innovative technologies for antigen delivery into the skin (such as microneedles), with the aim to improve vaccine efficacy alongside with enhanced patient adherence,  particularly  in  children  population  (noninvasive  or minimally  invasive administration). Therefore, this review deals with each of these approaches in more detail, with the special emphasis on examples of their use in vaccine formulations as well as on the factors influencing their efficacy and safety., U  doba  savremene  vakcinologije,  ograničena  imunogenost  većine  korišćenih  antigena podstakla  je  primenu  različitih  adjuvanasa  u  formulacijama  vakcina  radi  postizanja  željenog imunskog odgovora. Mineralni adjuvansi na bazi aluminijuma su istorijski najčešće korišćeni imunostimulansi u vakcinama i smatraju se generalno bezbednim za humanu upotrebu. Značajni napredak  na  polju  sinteze,  kontrole  strukture  i  funkcionalnog  dizajna  neorganskih (nano)materijala uslovio je povećano interesovanje za primenom inovativnih adjuvanasa kao što su  gline,  mezoporozne  silika  nanočestice,  nanočestice  cink-oksida,  gvožđe  oksida  i  gvožđe hidroksida, i dr. Sa druge strane, uočava se i sve veće interesovanje za primenom specifičnih nanonosača  antigena,  kao  što  su  nanoemulzije,  kako  bi  se  antigeni  zaštitili  od  preuranjene proteolitičke  degradacije  i/ili poboljšala  njihova  imunogenost,  olakšavanjem  preuzimanja  i obrade  od  strane  antigen-prezentujućih  ćelija.  Takođe,  brojni  istraživački  napori  tokom poslednjih nekoliko godina usmereni su ka razvoju inovativnih tehnologija za isporuku antigena u kožu (kao što su mikroigle) radi poboljšanja efikasnosti vakcinacije uz istovremeno povećanje adherence pacijenata, posebno u pedijatrijskoj populaciji (neinvazivan ili minimalno invazivan način primene). Otuda, u ovom preglednom radu dat je detaljan pregled karakteristika svakog od  navedenih  pristupa  za  poboljšanje  efikasnosti  vakcina,  sa  posebnim  osvrtom  na  primere njihove primene u formulacijama vakcina i faktore koji određuju efikasnost i bezbednost.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems, Konvencionalni i napredni adjuvansi u formulacijama vakcina: mineralni adsorbenti, nanočestični nosači i sistemi tipa mikroigala",
volume = "69",
number = "6",
pages = "420-451",
doi = "10.5937/arhfarm1906420K"
}

Krajišnik, D., Ilić, T., Nikolić, I.,& Savić, S.. (2019). Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 69(6), 420-451.
https://doi.org/10.5937/arhfarm1906420K

Krajišnik D, Ilić T, Nikolić I, Savić S. Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems. in Arhiv za farmaciju. 2019;69(6):420-451.
doi:10.5937/arhfarm1906420K .

Krajišnik, Danina, Ilić, Tanja, Nikolić, Ines, Savić, Snežana, "Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems" in Arhiv za farmaciju, 69, no. 6 (2019):420-451,
https://doi.org/10.5937/arhfarm1906420K . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Dobričić, Vladimir
AU  - Krajišnik, Danina
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5480
AB  - Ketotifen hidrogenfumarat (KF) je selektivni, nekompetitivni antagonist histamina (H1-
receptori) koji se koristi u lokalnoj terapiji alergijskog rinitisa i konjuktivitisa. Hitozan (H)
i hidroksipropil guar guma (HP GG) su biokompatibilni i biodegradabilni polimeri koji u
oftalmološkim preparatima zahvaljujući povećanju viskoziteta i mukoadhezivnim osobinama,
omogućavaju duži kontakt aktivne supstance sa rožnjačom i poboljšanje bioraspoloživosti.
Cilj ove studije bila su formulaciona i funkcionalna ispitivanja rastvora KF (0,025% m/v) za
oftalmološku primjenu koji sadrže H srednje molekulske mase (0,5% m/v) (uzorak F1), HP GG
(0,25% m/v) (uzorak F2) i njihovu kombinaciju (0,25 % m/v H/0,25% m/v HP GG) (uzorak F3) kao
modifikatore viskoziteta. Oftalmološki rastvori su pripremljeni miješanjem vehikuluma koji su
sadržavali polisaharide i pomoćne supstance (dibazni natrijum-fosfat, boraks i benzalkonijum-
hlorid) sa vodenim rastvorima KF. Izrađenim uzorcima ispitana je pH vrijednost, bistrina,
osmolalnost i sadržaj ljekovite supstance. Reološka karakterizacija sprovedena je na svježim
uzorcima na 20 °C i 34 °C (nakon razblaživanja sa vještačkom suznom tečnosti (VST) u odnosu
40:7) prije i nakon sterilizacije vodenom parom pod pritiskom. Sadržaj KF analiziran je HPLC
metodom.
Za sve ispitivane formulacije pH vrijednost, bistrina i osmolalnost bili su u prihvatljivom opsegu
za oftalmološke preparate. Vrijednosti viskoziteta (pri 20 ºC/100 s-1) bile su u opsegu od 13,2
mPa·s (F1) do 21 mPa·s (F3). Nakon razblaživanja sa VST i procesa sterilizacije, promjene
viskoziteta bile su manje od ±10% u odnosu na početne vrijednosti, za sve ispitivane formulacije.
Sadržaj KF nakon tri mjeseca bio je u opsegu 88-100% kod sterilisanih i nesterilisanih uzoraka.
Uspješno su formulisani viskozni rastvori KF za oftalmološku primjenu koji sadrže modifikatore
viskoziteta tipa polisaharida. Reproduktivni HPLC rezultati ukazuju na stabilnost KF i nakon
sterilizacije. Buduća istraživanja imaće za cilj ispitivanje lijek-polimer interakcija, uticaja izbora
pomoćnih supstanci kao i postupka izrade na funkcionalne karakteristike i stabilnost ispitivanih
rastvora za oftalmološku primjenu.
AB  - Ketotifen hydrogen fumarate (KF) is a selective and non-competitive histamine antagonist (H1-receptor) used topically in the treatment of allergic rhinitis and conjunctivitis. Chitosan (CH) and hydroxypropyl guar gum (HP GG), biocompatible and biodegradable polymers as mucoadhesive and viscosity increasing agents in ophthalmic products enable longer contact of active ingredient and the corneal surface, enhancing its bioavailability. The purpose of this study was formulation and functionality assessment of KF ophthalmic solutions (0.025% w/v) containing medium molecular weight CH (0.5% w/v) (sample F1), HP GG (0.25% w/v) (sample F2) and their combination (0.25% w/v CH/0.25% w/v HP GG) (sample F3) as viscosity modifiers. The ophthalmic solutions were prepared by mixing of solutions containing these polysaccharides, auxiliary substances (dibasic sodium phosphate, borax and benzalkonium chloride) and aqueous solutions of KF. The samples were evaluated for pH, clarity, osmolality and drug content. Rheological characterization was performed on fresh samples at 20 °C and 34 °C (after addition of simulated tear fluid (STF) in a ratio 40:7), before and after steam sterilization. The content of KF was analyzed by HPLC method. The pH, clarity and osmolality of all the tested formulations were within the acceptable range for ophthalmic preparations. The viscosity values (at 20 ºC/100 s-1) were in the range from 13.2 mPa·s (F1) to 21 mPa·s (F3). After dilution with STF and steam sterilization, changes of viscosity deviated less than ±10% compared with initial values for all tested vehicles. The drug content after 3 months was within range 88-100%, for both sterilized and non-sterilized samples. KF was successfully formulated in viscous ophthalmic solutions containing polysaccharidebased viscosity modifiers. Reproducible HPLC data revealed stability of KF after steam sterilization. Future research will be focused on investigations of drug-polymer interactions and influence of auxiliary substances selection alongside with preparation procedure on functional properties and ophthalmic solutions stability.
PB  - Prisma - korporativne komunikacije
PB  - Farmaceutska komora Crne Gore
C3  - Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka
T1  - Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta
T1  - Formulation and functionality assessment of ketotifen fumarate ophthalmic solutions containing polysaccharide-based viscosity modifiers
SP  - 192
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5480
ER  - 

@conference{
author = "Račić, Anđelka and Čalija, Bojan and Milić, Jela and Dobričić, Vladimir and Krajišnik, Danina",
year = "2019",
abstract = "Ketotifen hidrogenfumarat (KF) je selektivni, nekompetitivni antagonist histamina (H1-
receptori) koji se koristi u lokalnoj terapiji alergijskog rinitisa i konjuktivitisa. Hitozan (H)
i hidroksipropil guar guma (HP GG) su biokompatibilni i biodegradabilni polimeri koji u
oftalmološkim preparatima zahvaljujući povećanju viskoziteta i mukoadhezivnim osobinama,
omogućavaju duži kontakt aktivne supstance sa rožnjačom i poboljšanje bioraspoloživosti.
Cilj ove studije bila su formulaciona i funkcionalna ispitivanja rastvora KF (0,025% m/v) za
oftalmološku primjenu koji sadrže H srednje molekulske mase (0,5% m/v) (uzorak F1), HP GG
(0,25% m/v) (uzorak F2) i njihovu kombinaciju (0,25 % m/v H/0,25% m/v HP GG) (uzorak F3) kao
modifikatore viskoziteta. Oftalmološki rastvori su pripremljeni miješanjem vehikuluma koji su
sadržavali polisaharide i pomoćne supstance (dibazni natrijum-fosfat, boraks i benzalkonijum-
hlorid) sa vodenim rastvorima KF. Izrađenim uzorcima ispitana je pH vrijednost, bistrina,
osmolalnost i sadržaj ljekovite supstance. Reološka karakterizacija sprovedena je na svježim
uzorcima na 20 °C i 34 °C (nakon razblaživanja sa vještačkom suznom tečnosti (VST) u odnosu
40:7) prije i nakon sterilizacije vodenom parom pod pritiskom. Sadržaj KF analiziran je HPLC
metodom.
Za sve ispitivane formulacije pH vrijednost, bistrina i osmolalnost bili su u prihvatljivom opsegu
za oftalmološke preparate. Vrijednosti viskoziteta (pri 20 ºC/100 s-1) bile su u opsegu od 13,2
mPa·s (F1) do 21 mPa·s (F3). Nakon razblaživanja sa VST i procesa sterilizacije, promjene
viskoziteta bile su manje od ±10% u odnosu na početne vrijednosti, za sve ispitivane formulacije.
Sadržaj KF nakon tri mjeseca bio je u opsegu 88-100% kod sterilisanih i nesterilisanih uzoraka.
Uspješno su formulisani viskozni rastvori KF za oftalmološku primjenu koji sadrže modifikatore
viskoziteta tipa polisaharida. Reproduktivni HPLC rezultati ukazuju na stabilnost KF i nakon
sterilizacije. Buduća istraživanja imaće za cilj ispitivanje lijek-polimer interakcija, uticaja izbora
pomoćnih supstanci kao i postupka izrade na funkcionalne karakteristike i stabilnost ispitivanih
rastvora za oftalmološku primjenu., Ketotifen hydrogen fumarate (KF) is a selective and non-competitive histamine antagonist (H1-receptor) used topically in the treatment of allergic rhinitis and conjunctivitis. Chitosan (CH) and hydroxypropyl guar gum (HP GG), biocompatible and biodegradable polymers as mucoadhesive and viscosity increasing agents in ophthalmic products enable longer contact of active ingredient and the corneal surface, enhancing its bioavailability. The purpose of this study was formulation and functionality assessment of KF ophthalmic solutions (0.025% w/v) containing medium molecular weight CH (0.5% w/v) (sample F1), HP GG (0.25% w/v) (sample F2) and their combination (0.25% w/v CH/0.25% w/v HP GG) (sample F3) as viscosity modifiers. The ophthalmic solutions were prepared by mixing of solutions containing these polysaccharides, auxiliary substances (dibasic sodium phosphate, borax and benzalkonium chloride) and aqueous solutions of KF. The samples were evaluated for pH, clarity, osmolality and drug content. Rheological characterization was performed on fresh samples at 20 °C and 34 °C (after addition of simulated tear fluid (STF) in a ratio 40:7), before and after steam sterilization. The content of KF was analyzed by HPLC method. The pH, clarity and osmolality of all the tested formulations were within the acceptable range for ophthalmic preparations. The viscosity values (at 20 ºC/100 s-1) were in the range from 13.2 mPa·s (F1) to 21 mPa·s (F3). After dilution with STF and steam sterilization, changes of viscosity deviated less than ±10% compared with initial values for all tested vehicles. The drug content after 3 months was within range 88-100%, for both sterilized and non-sterilized samples. KF was successfully formulated in viscous ophthalmic solutions containing polysaccharidebased viscosity modifiers. Reproducible HPLC data revealed stability of KF after steam sterilization. Future research will be focused on investigations of drug-polymer interactions and influence of auxiliary substances selection alongside with preparation procedure on functional properties and ophthalmic solutions stability.",
publisher = "Prisma - korporativne komunikacije, Farmaceutska komora Crne Gore",
journal = "Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka",
title = "Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta, Formulation and functionality assessment of ketotifen fumarate ophthalmic solutions containing polysaccharide-based viscosity modifiers",
pages = "192-193",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5480"
}

Račić, A., Čalija, B., Milić, J., Dobričić, V.,& Krajišnik, D.. (2019). Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka
Prisma - korporativne komunikacije., 192-193.
https://hdl.handle.net/21.15107/rcub_farfar_5480

Račić A, Čalija B, Milić J, Dobričić V, Krajišnik D. Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka. 2019;:192-193.
https://hdl.handle.net/21.15107/rcub_farfar_5480 .

Račić, Anđelka, Čalija, Bojan, Milić, Jela, Dobričić, Vladimir, Krajišnik, Danina, "Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta" in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka (2019):192-193,
https://hdl.handle.net/21.15107/rcub_farfar_5480 .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Krajišnik, Danina
AU  - Milić, Jela
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3651
AB  - Owing to its safety, wide distribution, and unique physicochemical properties, water is indispensable in pharmaceutical industry as excipient, solvent for extraction, production of active ingredients and analytical reagents, cleaning and heat-transfer agent. Various water types are used for production of pharmaceutical preparations and the water quality requirements depend on characteristics and use of final pharmaceutical preparation and production stage at which is water used. This paper summarizes regulatory requirements related to water for pharmaceutical use and systems for water production, storage and distribution in pharmaceutical industry, with focus on current regulation changes in this field. An overview on types of water for pharmaceutical use is provided, especially that official in The European and The United States Pharmacopoeia, including their quality requirements, usage and production methods.
AB  - Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda je nezamenjiva u farmaceutskoj industriji kao ekscipijens, sredstvo za proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan je osobinama i namenom finalnog farmaceutskog preparata i fazom u postupku njegove proizvodnje u kojoj se voda koristi. U ovom radu su predstavljeni regulatorni zahtevi za vode za farmaceutsku upotrebu i sisteme za proizvodnju, distribuciju i čuvanje vode u farmaceutskoj industriji, sa posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu, sa fokusom na vode oficijalne u Evropskoj i Američkoj farmakopeji, uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Watertypesfor pharmaceuticaland quality requirementsuse – importance
T1  - Voda za farmaceutsku upotrebu – značaj, vrste i zahtevi za kvalitet
T1  - Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda  je  nezamenjiva  u  farmaceutskoj  industriji  kao  ekscipijens,  sredstvo  za  proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan  je  osobinama  i  namenom  finalnog  farmaceutskog  preparata  i  fazom  u  postupku njegove proizvodnje u kojoj se voda koristi.  U  ovom  radu  su  predstavljeni  regulatorni  zahtevi za  vode  za  farmaceutsku  upotrebu  i sisteme  za  proizvodnju,  distribuciju  i  čuvanje  vode  u  farmaceutskoj  industriji,  sa  posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu,  sa  fokusom  na  vode  oficinalne  u  Evropskoj  i  Američkoj  farmakopeji,  uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.
VL  - 69
IS  - 2
SP  - 90
EP  - 115
DO  - 10.5937/arhfarm1902090Q
ER  - 

@article{
author = "Čalija, Bojan and Krajišnik, Danina and Milić, Jela",
year = "2019",
abstract = "Owing to its safety, wide distribution, and unique physicochemical properties, water is indispensable in pharmaceutical industry as excipient, solvent for extraction, production of active ingredients and analytical reagents, cleaning and heat-transfer agent. Various water types are used for production of pharmaceutical preparations and the water quality requirements depend on characteristics and use of final pharmaceutical preparation and production stage at which is water used. This paper summarizes regulatory requirements related to water for pharmaceutical use and systems for water production, storage and distribution in pharmaceutical industry, with focus on current regulation changes in this field. An overview on types of water for pharmaceutical use is provided, especially that official in The European and The United States Pharmacopoeia, including their quality requirements, usage and production methods., Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda je nezamenjiva u farmaceutskoj industriji kao ekscipijens, sredstvo za proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan je osobinama i namenom finalnog farmaceutskog preparata i fazom u postupku njegove proizvodnje u kojoj se voda koristi. U ovom radu su predstavljeni regulatorni zahtevi za vode za farmaceutsku upotrebu i sisteme za proizvodnju, distribuciju i čuvanje vode u farmaceutskoj industriji, sa posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu, sa fokusom na vode oficijalne u Evropskoj i Američkoj farmakopeji, uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Watertypesfor pharmaceuticaland quality requirementsuse – importance, Voda za farmaceutsku upotrebu – značaj, vrste i zahtevi za kvalitet, Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda  je  nezamenjiva  u  farmaceutskoj  industriji  kao  ekscipijens,  sredstvo  za  proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan  je  osobinama  i  namenom  finalnog  farmaceutskog  preparata  i  fazom  u  postupku njegove proizvodnje u kojoj se voda koristi.  U  ovom  radu  su  predstavljeni  regulatorni  zahtevi za  vode  za  farmaceutsku  upotrebu  i sisteme  za  proizvodnju,  distribuciju  i  čuvanje  vode  u  farmaceutskoj  industriji,  sa  posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu,  sa  fokusom  na  vode  oficinalne  u  Evropskoj  i  Američkoj  farmakopeji,  uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.",
volume = "69",
number = "2",
pages = "90-115",
doi = "10.5937/arhfarm1902090Q"
}

Čalija, B., Krajišnik, D.,& Milić, J.. (2019). Watertypesfor pharmaceuticaland quality requirementsuse – importance. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 69(2), 90-115.
https://doi.org/10.5937/arhfarm1902090Q

Čalija B, Krajišnik D, Milić J. Watertypesfor pharmaceuticaland quality requirementsuse – importance. in Arhiv za farmaciju. 2019;69(2):90-115.
doi:10.5937/arhfarm1902090Q .

Čalija, Bojan, Krajišnik, Danina, Milić, Jela, "Watertypesfor pharmaceuticaland quality requirementsuse – importance" in Arhiv za farmaciju, 69, no. 2 (2019):90-115,
https://doi.org/10.5937/arhfarm1902090Q . .

TY  - JOUR
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Savić, Snežana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3490
AB  - Parenteralnim  putem  se  primenjuju  različiti  tipovi  farmaceutskih  preparata  čiji  sastav može  biti  jednostavan  (vodeni  rastvori)  i  manje  ili  više  kompleksan  (emulzije,  suspenzije, liposomi kao nosači lekovitih supstanci, čestični sistemi, čvrsti implanti/implantati). Napredak u bio-  i  nanotehnologiji  omogućio  je  razvoj  nove  klase  kompleksnih  lekova,  bioloških  i  tzv. nebioloških  kompleksnih  lekova  (i  njihovih  „similara”-  sličnih lekova),  čiji  dalji  razvoj  se očekuje u bliskoj budućnosti, a  koji se, u velikom broju slučajeva, primenjuju parenteralnim putem.  Parenteralni preparati koji u svom sastavu sadrže lekovite supstance koje su inkapsulirane u nosače tipa liposoma predstavljaju nebiološke kompleksne lekove koji su do sada najduže u upotrebi i čije su osobine i definisana svojstva kvaliteta najviše ispitivana. U radu je dat pregled obaveznih  i  dodatnih  (specifičnih) in  vitro  ispitivanja  liposomskih  nosača  lekovitih supstanci za primenu parenteralnim putem. Činjenica da postupci izvođenja ovih ispitivanja u  osnovi  nisu  propisani  u  relevantnim  farmakopejama  (Ph.  Eur., USP  i  JP)  i  da  se  mogu značajno razlikovati između laboratorija, doprinosi velikoj varijabilnosti dobijenih rezultata i ograničenjima u njihovom međusobnom poređenju. Regulatorna tela EMA i FDA učestvovala su u pripremi određenih dokumenata i razvoju odgovarajućih standarda i smernica u pogledu ispitivanja  kvaliteta  farmaceutskih  oblika  sa  liposomskim  nosačima  lekovitih  supstanci  za parenteralnu primenu.
AB  - A greater variety of pharmaceutical preparations can be administered by the parenteral route, the composition of which can be simple (aqueous solutions) and more or less complex (emulsions,  suspensions,  liposomes  as  carriers  of  active  pharmaceutical  ingredients,  particle systems,  solid  implants/implants).  In  addition,  advances  in  bio-  and  nano-  technology  have enabled the development of new classes of complex drugs, so-called non-biological complex drugs (and their similars) whose further development is expected in the near future, and which are in many cases applied by parenteral route.  Parenteral preparations containing active substances encapsulated in the liposome-type carriers represent a class of non-biological complex drugs which have the longest use so far and whose properties and defined quality characteristics are being most examined. In this paper, an overview  of  mandatory  and  additional  (specific) in  vitro  tests  for  parenteral  liposomal  drug carriers is presented. The fact that standard testing procedures are often not available in relevant pharmacopoeias  (Ph.  Eur.,  USP  and  JP),  so  that  they  can  vary  significantly  between laboratories, contributes to the great variability of the results obtained and constraints in their mutual  comparison.  EMA  and  FDA,  as  regulatory  agencies,  have  also  participated  in  the preparation of certain documents and development of appropriate standards and guidelines for quality control of liposomal drug carriers for parenteral application.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Izazovi in vitro karakterizacije nebioloških kompleksnih lekova - primer parenteralnih preparata sa liposomskim nosačima lekovitih supstanci
T1  - Challenges of in vitro characterization of non-biological complex drugs - example of parenteral preparations with liposomal drug carriers
VL  - 69
IS  - 3
SP  - 176
EP  - 198
DO  - 10.5937/arhfarm1903176K
ER  - 

@article{
author = "Krajišnik, Danina and Milić, Jela and Savić, Snežana",
year = "2019",
abstract = "Parenteralnim  putem  se  primenjuju  različiti  tipovi  farmaceutskih  preparata  čiji  sastav može  biti  jednostavan  (vodeni  rastvori)  i  manje  ili  više  kompleksan  (emulzije,  suspenzije, liposomi kao nosači lekovitih supstanci, čestični sistemi, čvrsti implanti/implantati). Napredak u bio-  i  nanotehnologiji  omogućio  je  razvoj  nove  klase  kompleksnih  lekova,  bioloških  i  tzv. nebioloških  kompleksnih  lekova  (i  njihovih  „similara”-  sličnih lekova),  čiji  dalji  razvoj  se očekuje u bliskoj budućnosti, a  koji se, u velikom broju slučajeva, primenjuju parenteralnim putem.  Parenteralni preparati koji u svom sastavu sadrže lekovite supstance koje su inkapsulirane u nosače tipa liposoma predstavljaju nebiološke kompleksne lekove koji su do sada najduže u upotrebi i čije su osobine i definisana svojstva kvaliteta najviše ispitivana. U radu je dat pregled obaveznih  i  dodatnih  (specifičnih) in  vitro  ispitivanja  liposomskih  nosača  lekovitih supstanci za primenu parenteralnim putem. Činjenica da postupci izvođenja ovih ispitivanja u  osnovi  nisu  propisani  u  relevantnim  farmakopejama  (Ph.  Eur., USP  i  JP)  i  da  se  mogu značajno razlikovati između laboratorija, doprinosi velikoj varijabilnosti dobijenih rezultata i ograničenjima u njihovom međusobnom poređenju. Regulatorna tela EMA i FDA učestvovala su u pripremi određenih dokumenata i razvoju odgovarajućih standarda i smernica u pogledu ispitivanja  kvaliteta  farmaceutskih  oblika  sa  liposomskim  nosačima  lekovitih  supstanci  za parenteralnu primenu., A greater variety of pharmaceutical preparations can be administered by the parenteral route, the composition of which can be simple (aqueous solutions) and more or less complex (emulsions,  suspensions,  liposomes  as  carriers  of  active  pharmaceutical  ingredients,  particle systems,  solid  implants/implants).  In  addition,  advances  in  bio-  and  nano-  technology  have enabled the development of new classes of complex drugs, so-called non-biological complex drugs (and their similars) whose further development is expected in the near future, and which are in many cases applied by parenteral route.  Parenteral preparations containing active substances encapsulated in the liposome-type carriers represent a class of non-biological complex drugs which have the longest use so far and whose properties and defined quality characteristics are being most examined. In this paper, an overview  of  mandatory  and  additional  (specific) in  vitro  tests  for  parenteral  liposomal  drug carriers is presented. The fact that standard testing procedures are often not available in relevant pharmacopoeias  (Ph.  Eur.,  USP  and  JP),  so  that  they  can  vary  significantly  between laboratories, contributes to the great variability of the results obtained and constraints in their mutual  comparison.  EMA  and  FDA,  as  regulatory  agencies,  have  also  participated  in  the preparation of certain documents and development of appropriate standards and guidelines for quality control of liposomal drug carriers for parenteral application.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Izazovi in vitro karakterizacije nebioloških kompleksnih lekova - primer parenteralnih preparata sa liposomskim nosačima lekovitih supstanci, Challenges of in vitro characterization of non-biological complex drugs - example of parenteral preparations with liposomal drug carriers",
volume = "69",
number = "3",
pages = "176-198",
doi = "10.5937/arhfarm1903176K"
}

Krajišnik, D., Milić, J.,& Savić, S.. (2019). Izazovi in vitro karakterizacije nebioloških kompleksnih lekova - primer parenteralnih preparata sa liposomskim nosačima lekovitih supstanci. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 69(3), 176-198.
https://doi.org/10.5937/arhfarm1903176K

Krajišnik D, Milić J, Savić S. Izazovi in vitro karakterizacije nebioloških kompleksnih lekova - primer parenteralnih preparata sa liposomskim nosačima lekovitih supstanci. in Arhiv za farmaciju. 2019;69(3):176-198.
doi:10.5937/arhfarm1903176K .

Krajišnik, Danina, Milić, Jela, Savić, Snežana, "Izazovi in vitro karakterizacije nebioloških kompleksnih lekova - primer parenteralnih preparata sa liposomskim nosačima lekovitih supstanci" in Arhiv za farmaciju, 69, no. 3 (2019):176-198,
https://doi.org/10.5937/arhfarm1903176K . .

TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Fahr, Alfred
AU  - Maibach, Howard
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3319
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3438
AB  - Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements
VL  - 45
IS  - 1
SP  - 43
EP  - 54
DO  - 10.1080/03639045.2018.1515220
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Fahr, Alfred and Maibach, Howard",
year = "2019",
abstract = "Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements",
volume = "45",
number = "1",
pages = "43-54",
doi = "10.1080/03639045.2018.1515220"
}

Dragičević, N., Krajišnik, D., Milić, J., Fahr, A.,& Maibach, H.. (2019). Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 45(1), 43-54.
https://doi.org/10.1080/03639045.2018.1515220

Dragičević N, Krajišnik D, Milić J, Fahr A, Maibach H. Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy. 2019;45(1):43-54.
doi:10.1080/03639045.2018.1515220 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Fahr, Alfred, Maibach, Howard, "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements" in Drug Development and Industrial Pharmacy, 45, no. 1 (2019):43-54,
https://doi.org/10.1080/03639045.2018.1515220 . .