TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Marković, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4747
AB  - Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Prospective study of cytotoxic and genotoxic effects of Combretastatin
SP  - 133
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4747
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Topalović, Dijana and Živković, Lada and Marković, Milica and Pirković, Andrea",
year = "2023",
abstract = "Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Prospective study of cytotoxic and genotoxic effects of Combretastatin",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4747"
}

Spremo-Potparević, B., Topalović, D., Živković, L., Marković, M.,& Pirković, A.. (2023). Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747

Spremo-Potparević B, Topalović D, Živković L, Marković M, Pirković A. Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747 .

Spremo-Potparević, Biljana, Topalović, Dijana, Živković, Lada, Marković, Milica, Pirković, Andrea, "Prospective study of cytotoxic and genotoxic effects of Combretastatin" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):133-133,
https://hdl.handle.net/21.15107/rcub_farfar_4747 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4919
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 

@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}

Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J. M.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis
Oxford University Press., 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013

Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković JM. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .

Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan M., "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5185
AB  - Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.
PB  - Macedonian Academy of Sciences and Arts
C3  - Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
T1  - Evaluation of the antioxidant potential of Biochaga in vitro
VL  - 26
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5185
ER  - 

@conference{
author = "Živković, Lada and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)",
title = "Evaluation of the antioxidant potential of Biochaga in vitro",
volume = "26",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5185"
}

Živković, L., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
Macedonian Academy of Sciences and Arts., 26, 146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185

Živković L, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book). 2023;26:146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185 .

Živković, Lada, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of the antioxidant potential of Biochaga in vitro" in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book), 26 (2023):146-146,
https://hdl.handle.net/21.15107/rcub_farfar_5185 .

TY  - CONF
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4748
AB  - Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist
SP  - 46
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4748
ER  - 

@conference{
author = "Živković, Lada and Borozan, Sunčica and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4748"
}

Živković, L., Borozan, S., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748

Živković L, Borozan S, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748 .

Živković, Lada, Borozan, Sunčica, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):46-46,
https://hdl.handle.net/21.15107/rcub_farfar_4748 .

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kotur-Stevuljević, Jelena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4618
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4618
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kotur-Stevuljević, Jelena and Samardžić, Stevan and Maksimović, Zoran",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4618"
}

Spremo-Potparević, B., Živković, L., Topalović, D., Kotur-Stevuljević, J., Samardžić, S.,& Maksimović, Z.. (2022). Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618

Spremo-Potparević B, Živković L, Topalović D, Kotur-Stevuljević J, Samardžić S, Maksimović Z. Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618 .

Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kotur-Stevuljević, Jelena, Samardžić, Stevan, Maksimović, Zoran, "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):90-90,
https://hdl.handle.net/21.15107/rcub_farfar_4618 .

TY  - JOUR
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Pirković, Andrea
AU  - Periša, Jovana
AU  - Murafa, Natalija
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Zarubica, Aleksandra
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4281
AB  - The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control
PB  - MDPI
T2  - Nanomaterials
T1  - Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin
VL  - 12
IS  - 18
DO  - 10.3390/nano12183195
ER  - 

@article{
author = "Sredojević, Dušan and Lazić, Vesna and Pirković, Andrea and Periša, Jovana and Murafa, Natalija and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Zarubica, Aleksandra and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2022",
abstract = "The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control",
publisher = "MDPI",
journal = "Nanomaterials",
title = "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin",
volume = "12",
number = "18",
doi = "10.3390/nano12183195"
}

Sredojević, D., Lazić, V., Pirković, A., Periša, J., Murafa, N., Spremo-Potparević, B., Živković, L., Topalović, D., Zarubica, A., Jovanović Krivokuća, M.,& Nedeljković, J.. (2022). Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials
MDPI., 12(18).
https://doi.org/10.3390/nano12183195

Sredojević D, Lazić V, Pirković A, Periša J, Murafa N, Spremo-Potparević B, Živković L, Topalović D, Zarubica A, Jovanović Krivokuća M, Nedeljković J. Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials. 2022;12(18).
doi:10.3390/nano12183195 .

Sredojević, Dušan, Lazić, Vesna, Pirković, Andrea, Periša, Jovana, Murafa, Natalija, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Zarubica, Aleksandra, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin" in Nanomaterials, 12, no. 18 (2022),
https://doi.org/10.3390/nano12183195 . .

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Dimitrijević Srećković, Vesna
AU  - Pirković, Andrea
AU  - Petrović, Hristina
AU  - Spremo-Potparević, Biljana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4630
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro
SP  - 94
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4630
ER  - 

@conference{
author = "Živković, Lada and Topalović, Dijana and Borozan, Sunčica and Bajić, Vladan and Dimitrijević Srećković, Vesna and Pirković, Andrea and Petrović, Hristina and Spremo-Potparević, Biljana",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro",
pages = "94-94",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4630"
}

Živković, L., Topalović, D., Borozan, S., Bajić, V., Dimitrijević Srećković, V., Pirković, A., Petrović, H.,& Spremo-Potparević, B.. (2022). Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630

Živković L, Topalović D, Borozan S, Bajić V, Dimitrijević Srećković V, Pirković A, Petrović H, Spremo-Potparević B. Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

Živković, Lada, Topalović, Dijana, Borozan, Sunčica, Bajić, Vladan, Dimitrijević Srećković, Vesna, Pirković, Andrea, Petrović, Hristina, Spremo-Potparević, Biljana, "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):94-94,
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

TY  - JOUR
AU  - Mićović, Tijana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Jakovljević, Vladimir
AU  - Matić, Sanja
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Stešević, Danijela
AU  - Samardžić, Stevan
AU  - Stojanović, Danilo
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3817
AB  - Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.
PB  - MDPI AG
T2  - Plants
T1  - Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)
VL  - 10
IS  - 4
DO  - 10.3390/plants10040711
ER  - 

@article{
author = "Mićović, Tijana and Topalović, Dijana and Živković, Lada and Spremo-Potparević, Biljana and Jakovljević, Vladimir and Matić, Sanja and Popović, Suzana and Baskić, Dejan and Stešević, Danijela and Samardžić, Stevan and Stojanović, Danilo and Maksimović, Zoran",
year = "2021",
abstract = "Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)",
volume = "10",
number = "4",
doi = "10.3390/plants10040711"
}

Mićović, T., Topalović, D., Živković, L., Spremo-Potparević, B., Jakovljević, V., Matić, S., Popović, S., Baskić, D., Stešević, D., Samardžić, S., Stojanović, D.,& Maksimović, Z.. (2021). Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants
MDPI AG., 10(4).
https://doi.org/10.3390/plants10040711

Mićović T, Topalović D, Živković L, Spremo-Potparević B, Jakovljević V, Matić S, Popović S, Baskić D, Stešević D, Samardžić S, Stojanović D, Maksimović Z. Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants. 2021;10(4).
doi:10.3390/plants10040711 .

Mićović, Tijana, Topalović, Dijana, Živković, Lada, Spremo-Potparević, Biljana, Jakovljević, Vladimir, Matić, Sanja, Popović, Suzana, Baskić, Dejan, Stešević, Danijela, Samardžić, Stevan, Stojanović, Danilo, Maksimović, Zoran, "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)" in Plants, 10, no. 4 (2021),
https://doi.org/10.3390/plants10040711 . .

TY  - CHAP
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5184
AB  - Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.
PB  - Elsevier
T2  - Olives and Olive Oil in Health and Disease Prevention
T1  - Olive leaf, DNA damage and chelation therapy
SP  - 457
EP  - 469
DO  - 10.1016/B978-0-12-819528-4.00021-3
ER  - 

@inbook{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.",
publisher = "Elsevier",
journal = "Olives and Olive Oil in Health and Disease Prevention",
booktitle = "Olive leaf, DNA damage and chelation therapy",
pages = "457-469",
doi = "10.1016/B978-0-12-819528-4.00021-3"
}

Čabarkapa-Pirković, A., Živković, L., Dekanski, D., Topalović, D.,& Spremo-Potparević, B.. (2021). Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention
Elsevier., 457-469.
https://doi.org/10.1016/B978-0-12-819528-4.00021-3

Čabarkapa-Pirković A, Živković L, Dekanski D, Topalović D, Spremo-Potparević B. Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention. 2021;:457-469.
doi:10.1016/B978-0-12-819528-4.00021-3 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Spremo-Potparević, Biljana, "Olive leaf, DNA damage and chelation therapy" in Olives and Olive Oil in Health and Disease Prevention (2021):457-469,
https://doi.org/10.1016/B978-0-12-819528-4.00021-3 . .

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920
AB  - Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
AB  - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
T1  - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
VL  - 71
IS  - 3
SP  - 197
EP  - 206
DO  - 10.5937/arhfarm71‐31060
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada",
year = "2021",
abstract = "Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka",
volume = "71",
number = "3",
pages = "197-206",
doi = "10.5937/arhfarm71‐31060"
}

Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206.
https://doi.org/10.5937/arhfarm71‐31060

Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206.
doi:10.5937/arhfarm71‐31060 .

Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206,
https://doi.org/10.5937/arhfarm71‐31060 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3900
AB  - Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay
T1  - Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay, Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis
Serbian Medical Society., 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798

Topalović D, Živković L, Đelić N, Bajić V, Spremo-Potparević B. Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay" in Medicinski Casopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - CONF
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5187
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Turkish Society of Toxicology
C3  - 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
T1  - Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5187
ER  - 

@conference{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Turkish Society of Toxicology",
journal = "10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book",
title = "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5187"
}

Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
Turkish Society of Toxicology., 169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187

Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187 .

Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC" in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book (2019):169-169,
https://hdl.handle.net/21.15107/rcub_farfar_5187 .

TY  - CONF
AU  - Burja, Blaž
AU  - Topalović, Dijana
AU  - Kuret, Tadeja
AU  - Živković, Lada
AU  - Mrak-Poljšak, Katjuša
AU  - Janko, Tea
AU  - Spremo-Potparević, Biljana
AU  - Žigon, Polona
AU  - Čučnik, Saša
AU  - Sodin-Šemrl, Snežna
AU  - Lakota, Katja
AU  - Frank-Bertoncelj, Mojca
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5195
AB  - Background and Aims: Olive leaf extract (OLE) has been traditionally used due to its anti-inflammatory and anti-atherogenic effects.  ...
PB  - Elsevier
C3  - Atherosclerosis
T1  - Protective Effects Of Olive Leaf Extract On Inflammatory Activation Of Endothelial Cells
VL  - 287
SP  - e95
EP  - e95
DO  - 10.1016/j.atherosclerosis.2019.06.276
ER  - 

@conference{
author = "Burja, Blaž and Topalović, Dijana and Kuret, Tadeja and Živković, Lada and Mrak-Poljšak, Katjuša and Janko, Tea and Spremo-Potparević, Biljana and Žigon, Polona and Čučnik, Saša and Sodin-Šemrl, Snežna and Lakota, Katja and Frank-Bertoncelj, Mojca",
year = "2019",
abstract = "Background and Aims: Olive leaf extract (OLE) has been traditionally used due to its anti-inflammatory and anti-atherogenic effects.  ...",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Protective Effects Of Olive Leaf Extract On Inflammatory Activation Of Endothelial Cells",
volume = "287",
pages = "e95-e95",
doi = "10.1016/j.atherosclerosis.2019.06.276"
}

Burja, B., Topalović, D., Kuret, T., Živković, L., Mrak-Poljšak, K., Janko, T., Spremo-Potparević, B., Žigon, P., Čučnik, S., Sodin-Šemrl, S., Lakota, K.,& Frank-Bertoncelj, M.. (2019). Protective Effects Of Olive Leaf Extract On Inflammatory Activation Of Endothelial Cells. in Atherosclerosis
Elsevier., 287, e95-e95.
https://doi.org/10.1016/j.atherosclerosis.2019.06.276

Burja B, Topalović D, Kuret T, Živković L, Mrak-Poljšak K, Janko T, Spremo-Potparević B, Žigon P, Čučnik S, Sodin-Šemrl S, Lakota K, Frank-Bertoncelj M. Protective Effects Of Olive Leaf Extract On Inflammatory Activation Of Endothelial Cells. in Atherosclerosis. 2019;287:e95-e95.
doi:10.1016/j.atherosclerosis.2019.06.276 .

Burja, Blaž, Topalović, Dijana, Kuret, Tadeja, Živković, Lada, Mrak-Poljšak, Katjuša, Janko, Tea, Spremo-Potparević, Biljana, Žigon, Polona, Čučnik, Saša, Sodin-Šemrl, Snežna, Lakota, Katja, Frank-Bertoncelj, Mojca, "Protective Effects Of Olive Leaf Extract On Inflammatory Activation Of Endothelial Cells" in Atherosclerosis, 287 (2019):e95-e95,
https://doi.org/10.1016/j.atherosclerosis.2019.06.276 . .

TY  - CONF
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5192
AB  - The binding of thyroid hormones to specific nuclear receptors in target cells induces synthesis of enzymes associated with redox processes, leading to the formation of reactive oxygen species (ROS), which can cause damage of DNA molecule. Quercetin has already been shown to have protective effect against DNA damage, with its most pronounced feature being scavenging of free radicals. The aim of this study was to evaluate antigenotoxic potential of quercetin against thyroxine-induced DNA damage in human whole blood cells by using the comet assay. For that purpose, cells were exposed to 50 μM thyroxine and separately pre-treated or post treated with 500 μM of quercetin. Results showed that DNA damage was significantly reduced in cells pre-treated with this scavenger of free radicals. Obtained results indicate the ability of thyroxine to be a mediator of DNA damage and that quercetin displayed protective effect against thyroxine-induced genotoxicity.
PB  - Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Genetics & Applications
T1  - Antigenotoxic effect of quercetin on thyroxine-induced DNA damage in human whole blood cells in vitro
VL  - 3
IS  - 2
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5192
ER  - 

@conference{
author = "Topalović, Dijana and Živković, Lada and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The binding of thyroid hormones to specific nuclear receptors in target cells induces synthesis of enzymes associated with redox processes, leading to the formation of reactive oxygen species (ROS), which can cause damage of DNA molecule. Quercetin has already been shown to have protective effect against DNA damage, with its most pronounced feature being scavenging of free radicals. The aim of this study was to evaluate antigenotoxic potential of quercetin against thyroxine-induced DNA damage in human whole blood cells by using the comet assay. For that purpose, cells were exposed to 50 μM thyroxine and separately pre-treated or post treated with 500 μM of quercetin. Results showed that DNA damage was significantly reduced in cells pre-treated with this scavenger of free radicals. Obtained results indicate the ability of thyroxine to be a mediator of DNA damage and that quercetin displayed protective effect against thyroxine-induced genotoxicity.",
publisher = "Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Genetics & Applications",
title = "Antigenotoxic effect of quercetin on thyroxine-induced DNA damage in human whole blood cells in vitro",
volume = "3",
number = "2",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5192"
}

Topalović, D., Živković, L., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic effect of quercetin on thyroxine-induced DNA damage in human whole blood cells in vitro. in Genetics & Applications
Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 3(2), 88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5192

Topalović D, Živković L, Bruić M, Spremo-Potparević B. Antigenotoxic effect of quercetin on thyroxine-induced DNA damage in human whole blood cells in vitro. in Genetics & Applications. 2019;3(2):88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5192 .

Topalović, Dijana, Živković, Lada, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic effect of quercetin on thyroxine-induced DNA damage in human whole blood cells in vitro" in Genetics & Applications, 3, no. 2 (2019):88-88,
https://hdl.handle.net/21.15107/rcub_farfar_5192 .

TY  - CONF
AU  - Bruić, Marija
AU  - Grujić-Milanović, Jelica
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5194
AB  - Hypertension is one of the primary risk factors for heart disease and stroke, the leading causes of death worldwide. Numerous factors have been implicated in the pathophysiology of hypertension: endothelial dysfunction, arterial remodeling and vascular inflammation. Common to all these processes is increased bioavailability of reactive oxygen species in the vessels, heart, brain and kidneys. Oxidative stress and increased reactive oxygen species levels damage all macromolecules, with DNA being particularly susceptible to oxidative damage. The aim of this study was to determine whether there is a difference in the level of DNA damage between normotensive and hypertensive rats using the alkaline comet assay. Blood samples and cells suspension from liver and kidney from three male spontaneously hypertensive rats were obtained. Three normotensive male Wistar rats were used as a control. Increased level of DNA damage was detected in blood and both of the studied tissues of hypertensive rats compared to the control, where significant difference was present in the liver and kidney cell suspensions. These results indicate that untreated hypertension in rats leads to an increased DNA damage in all of the studied samples, detected by comet assay.
PB  - Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Genetics & Applications
T1  - Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay
VL  - 3
IS  - 2
SP  - 89
EP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5194
ER  - 

@conference{
author = "Bruić, Marija and Grujić-Milanović, Jelica and Živković, Lada and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Hypertension is one of the primary risk factors for heart disease and stroke, the leading causes of death worldwide. Numerous factors have been implicated in the pathophysiology of hypertension: endothelial dysfunction, arterial remodeling and vascular inflammation. Common to all these processes is increased bioavailability of reactive oxygen species in the vessels, heart, brain and kidneys. Oxidative stress and increased reactive oxygen species levels damage all macromolecules, with DNA being particularly susceptible to oxidative damage. The aim of this study was to determine whether there is a difference in the level of DNA damage between normotensive and hypertensive rats using the alkaline comet assay. Blood samples and cells suspension from liver and kidney from three male spontaneously hypertensive rats were obtained. Three normotensive male Wistar rats were used as a control. Increased level of DNA damage was detected in blood and both of the studied tissues of hypertensive rats compared to the control, where significant difference was present in the liver and kidney cell suspensions. These results indicate that untreated hypertension in rats leads to an increased DNA damage in all of the studied samples, detected by comet assay.",
publisher = "Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Genetics & Applications",
title = "Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay",
volume = "3",
number = "2",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5194"
}

Bruić, M., Grujić-Milanović, J., Živković, L., Topalović, D.,& Spremo-Potparević, B.. (2019). Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay. in Genetics & Applications
Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 3(2), 89-89.
https://hdl.handle.net/21.15107/rcub_farfar_5194

Bruić M, Grujić-Milanović J, Živković L, Topalović D, Spremo-Potparević B. Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay. in Genetics & Applications. 2019;3(2):89-89.
https://hdl.handle.net/21.15107/rcub_farfar_5194 .

Bruić, Marija, Grujić-Milanović, Jelica, Živković, Lada, Topalović, Dijana, Spremo-Potparević, Biljana, "Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay" in Genetics & Applications, 3, no. 2 (2019):89-89,
https://hdl.handle.net/21.15107/rcub_farfar_5194 .

TY  - CONF
AU  - Bruić, Marija
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5151
AB  - Inonotus obliquus, edible medicinal mushroom found in northern hemisphere, is widely used for the prevention and treatment of cancer, diabetes, colitis and asthma. Its bioactives, triterpens, polysaccharides and polyphenols exhibit antitumor, anti-inflamatory, hypoglycemic, immunomodulatory effects, as well as antioxidative properties. Oxidative stress and increased ROS levels lead to tissue damage, with DNA molecule being particularly susceptible to oxidative damage. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow
T1  - Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro
SP  - 54
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5151
ER  - 

@conference{
author = "Bruić, Marija and Živković, Lada and Bajić, Vladan and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Inonotus obliquus, edible medicinal mushroom found in northern hemisphere, is widely used for the prevention and treatment of cancer, diabetes, colitis and asthma. Its bioactives, triterpens, polysaccharides and polyphenols exhibit antitumor, anti-inflamatory, hypoglycemic, immunomodulatory effects, as well as antioxidative properties. Oxidative stress and increased ROS levels lead to tissue damage, with DNA molecule being particularly susceptible to oxidative damage. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow",
title = "Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro",
pages = "54-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5151"
}

Bruić, M., Živković, L., Bajić, V., Topalović, D.,& Spremo-Potparević, B.. (2019). Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro. in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow
International Comet Assay Working Group (ICAWG)., 54-54.
https://hdl.handle.net/21.15107/rcub_farfar_5151

Bruić M, Živković L, Bajić V, Topalović D, Spremo-Potparević B. Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro. in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow. 2019;:54-54.
https://hdl.handle.net/21.15107/rcub_farfar_5151 .

Bruić, Marija, Živković, Lada, Bajić, Vladan, Topalović, Dijana, Spremo-Potparević, Biljana, "Evaluation of repair kinetics of oxidative damaged DNA in peripheral blood leukocytes treated with chaga mushroom (Inonotus obliquus) extract in vitro" in 13th International Comet Assay Workshop, 24-27 June, 2019, Pushchino, Moscow (2019):54-54,
https://hdl.handle.net/21.15107/rcub_farfar_5151 .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3484
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Hindawi
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
DO  - 10.1155/2019/5039372
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Hindawi",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
doi = "10.1155/2019/5039372"
}

Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi., 2019.
https://doi.org/10.1155/2019/5039372

Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/5039372 .

Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/5039372 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3459
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3456
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Lazić, Vesna
AU  - Vukoje, Ivana
AU  - Milicević, Bojana
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan M.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3257
AB  - The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis
VL  - 84
IS  - 6
SP  - 539
EP  - 553
DO  - 10.2298/JSC181217017L
ER  - 

@article{
author = "Lazić, Vesna and Vukoje, Ivana and Milicević, Bojana and Potparević, Biljana and Živković, Lada and Topalović, Dijana and Bajić, Vladan and Sredojević, Dušan and Nedeljković, Jovan M.",
year = "2019",
abstract = "The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis",
volume = "84",
number = "6",
pages = "539-553",
doi = "10.2298/JSC181217017L"
}

Lazić, V., Vukoje, I., Milicević, B., Potparević, B., Živković, L., Topalović, D., Bajić, V., Sredojević, D.,& Nedeljković, J. M.. (2019). Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(6), 539-553.
https://doi.org/10.2298/JSC181217017L

Lazić V, Vukoje I, Milicević B, Potparević B, Živković L, Topalović D, Bajić V, Sredojević D, Nedeljković JM. Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society. 2019;84(6):539-553.
doi:10.2298/JSC181217017L .

Lazić, Vesna, Vukoje, Ivana, Milicević, Bojana, Potparević, Biljana, Živković, Lada, Topalović, Dijana, Bajić, Vladan, Sredojević, Dušan, Nedeljković, Jovan M., "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis" in Journal of the Serbian Chemical Society, 84, no. 6 (2019):539-553,
https://doi.org/10.2298/JSC181217017L . .

TY  - JOUR
AU  - Burja, Blaz
AU  - Kuret, Tadeja
AU  - Janko, Tea
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Mrak-Poljsak, Katjusa
AU  - Potparević, Biljana
AU  - Zigon, Polona
AU  - Distler, Oliver
AU  - Cucnik, Sasa
AU  - Sodin-Semrl, Snezna
AU  - Lakota, Katja
AU  - Frank-Bertoncelj, Mojca
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3344
AB  - Olive leaf extract (OLE) is used in traditional medicine as a food supplement and as an over-the-counter drug for a variety of its effects, including anti-inflammatory and anti-atherosclerotic ones. Mechanisms through which OLE could modulate these pathways in human vasculature remain largely unknown. Serum amyloid A (SAA) plays a causal role in atherosclerosis and cardiovascular diseases and induces pro-inflammatory and pro-adhesive responses in human coronary artery endothelial cells (HCAEC). Within this study we explored whether OLE can attenuate SAA-driven responses in HCAEC. HCAEC were treated with SAA (1,000 nM) and/or OLE (0.5 and 1 mg/ml). The expression of adhesion molecules VCAM-1 and E-selectin, matrix metalloproteinases (MMP2 and MMP9) and microRNA 146a, let-7e, and let-7g (involved in the regulation of inflammation) was determined by qPCR. The amount of secreted IL-6, IL-8, MIF, and GRO-alpha in cell culture supernatants was quantified by ELISA. Phosphorylation of NF-kappa B was assessed by Western blot and DNA damage was measured using the COMET assay. OLE decreased significantly released protein levels of IL-6 and IL-8, as well as mRNA expression of E-selectin in SAA-stimulated HCAEC and reduced MMP2 levels in unstimulated cells. Phosphorylation of NF-kappa B (p65) was upregulated in the presence of SAA, with OLE significantly attenuating this SAA-induced effect. OLE stabilized SAA-induced upregulation of microRNA-146a and let-7e in HCAEC, suggesting that OLE could fine-tune the SAA-driven activity of NF-kappa B by changing the microRNA networks in HCAEC. SAA induced DNA damage and worsened the oxidative DNA damage in HCAEC, whereas OLE protected HCAEC from SAA- and H2O2-driven DNA damage. OLE significantly attenuated certain pro-inflammatory and pro-adhesive responses and decreased DNA damage in HCAEC upon stimulation with SAA. The reversal of SAA-driven endothelial activation by OLE might contribute to its anti-inflammatory and anti-atherogenic effects in HCAEC.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cardiovascular Medicine
T1  - Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells
VL  - 6
DO  - 10.3389/fcvm.2019.00056
ER  - 

@article{
author = "Burja, Blaz and Kuret, Tadeja and Janko, Tea and Topalović, Dijana and Živković, Lada and Mrak-Poljsak, Katjusa and Potparević, Biljana and Zigon, Polona and Distler, Oliver and Cucnik, Sasa and Sodin-Semrl, Snezna and Lakota, Katja and Frank-Bertoncelj, Mojca",
year = "2019",
abstract = "Olive leaf extract (OLE) is used in traditional medicine as a food supplement and as an over-the-counter drug for a variety of its effects, including anti-inflammatory and anti-atherosclerotic ones. Mechanisms through which OLE could modulate these pathways in human vasculature remain largely unknown. Serum amyloid A (SAA) plays a causal role in atherosclerosis and cardiovascular diseases and induces pro-inflammatory and pro-adhesive responses in human coronary artery endothelial cells (HCAEC). Within this study we explored whether OLE can attenuate SAA-driven responses in HCAEC. HCAEC were treated with SAA (1,000 nM) and/or OLE (0.5 and 1 mg/ml). The expression of adhesion molecules VCAM-1 and E-selectin, matrix metalloproteinases (MMP2 and MMP9) and microRNA 146a, let-7e, and let-7g (involved in the regulation of inflammation) was determined by qPCR. The amount of secreted IL-6, IL-8, MIF, and GRO-alpha in cell culture supernatants was quantified by ELISA. Phosphorylation of NF-kappa B was assessed by Western blot and DNA damage was measured using the COMET assay. OLE decreased significantly released protein levels of IL-6 and IL-8, as well as mRNA expression of E-selectin in SAA-stimulated HCAEC and reduced MMP2 levels in unstimulated cells. Phosphorylation of NF-kappa B (p65) was upregulated in the presence of SAA, with OLE significantly attenuating this SAA-induced effect. OLE stabilized SAA-induced upregulation of microRNA-146a and let-7e in HCAEC, suggesting that OLE could fine-tune the SAA-driven activity of NF-kappa B by changing the microRNA networks in HCAEC. SAA induced DNA damage and worsened the oxidative DNA damage in HCAEC, whereas OLE protected HCAEC from SAA- and H2O2-driven DNA damage. OLE significantly attenuated certain pro-inflammatory and pro-adhesive responses and decreased DNA damage in HCAEC upon stimulation with SAA. The reversal of SAA-driven endothelial activation by OLE might contribute to its anti-inflammatory and anti-atherogenic effects in HCAEC.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cardiovascular Medicine",
title = "Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells",
volume = "6",
doi = "10.3389/fcvm.2019.00056"
}

Burja, B., Kuret, T., Janko, T., Topalović, D., Živković, L., Mrak-Poljsak, K., Potparević, B., Zigon, P., Distler, O., Cucnik, S., Sodin-Semrl, S., Lakota, K.,& Frank-Bertoncelj, M.. (2019). Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells. in Frontiers in Cardiovascular Medicine
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fcvm.2019.00056

Burja B, Kuret T, Janko T, Topalović D, Živković L, Mrak-Poljsak K, Potparević B, Zigon P, Distler O, Cucnik S, Sodin-Semrl S, Lakota K, Frank-Bertoncelj M. Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells. in Frontiers in Cardiovascular Medicine. 2019;6.
doi:10.3389/fcvm.2019.00056 .

Burja, Blaz, Kuret, Tadeja, Janko, Tea, Topalović, Dijana, Živković, Lada, Mrak-Poljsak, Katjusa, Potparević, Biljana, Zigon, Polona, Distler, Oliver, Cucnik, Sasa, Sodin-Semrl, Snezna, Lakota, Katja, Frank-Bertoncelj, Mojca, "Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells" in Frontiers in Cardiovascular Medicine, 6 (2019),
https://doi.org/10.3389/fcvm.2019.00056 . .

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3165
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 

@article{
author = "Dekanski, Dragana and Potparević, Biljana and Bajić, Vladan and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna and Nedeljković, Jovan",
year = "2018",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}

Dekanski, D., Potparević, B., Bajić, V., Živković, L., Topalović, D., Sredojević, D., Lazić, V.,& Nedeljković, J.. (2018). Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064

Dekanski D, Potparević B, Bajić V, Živković L, Topalović D, Sredojević D, Lazić V, Nedeljković J. Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology. 2018;115:42-48.
doi:10.1016/j.fct.2018.02.064 .

Dekanski, Dragana, Potparević, Biljana, Bajić, Vladan, Živković, Lada, Topalović, Dijana, Sredojević, Dušan, Lazić, Vesna, Nedeljković, Jovan, "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid" in Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Lunter, Dominique
AU  - Ranđelović, Danijela
AU  - Žugić, Ana R.
AU  - Tadić, Vanja M.
AU  - Marković, Bojan
AU  - Cekić, Nebojša
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Potparević, Biljana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3084
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application
VL  - 550
IS  - 1-2
SP  - 333
EP  - 346
DO  - 10.1016/j.ijpharm.2018.08.060
ER  - 

@article{
author = "Nikolić, Ines and Lunter, Dominique and Ranđelović, Danijela and Žugić, Ana R. and Tadić, Vanja M. and Marković, Bojan and Cekić, Nebojša and Živković, Lada and Topalović, Dijana and Potparević, Biljana and Daniels, Rolf and Savić, Snežana",
year = "2018",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application",
volume = "550",
number = "1-2",
pages = "333-346",
doi = "10.1016/j.ijpharm.2018.08.060"
}

Nikolić, I., Lunter, D., Ranđelović, D., Žugić, A. R., Tadić, V. M., Marković, B., Cekić, N., Živković, L., Topalović, D., Potparević, B., Daniels, R.,& Savić, S.. (2018). Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 550(1-2), 333-346.
https://doi.org/10.1016/j.ijpharm.2018.08.060

Nikolić I, Lunter D, Ranđelović D, Žugić AR, Tadić VM, Marković B, Cekić N, Živković L, Topalović D, Potparević B, Daniels R, Savić S. Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics. 2018;550(1-2):333-346.
doi:10.1016/j.ijpharm.2018.08.060 .

Nikolić, Ines, Lunter, Dominique, Ranđelović, Danijela, Žugić, Ana R., Tadić, Vanja M., Marković, Bojan, Cekić, Nebojša, Živković, Lada, Topalović, Dijana, Potparević, Biljana, Daniels, Rolf, Savić, Snežana, "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application" in International Journal of Pharmaceutics, 550, no. 1-2 (2018):333-346,
https://doi.org/10.1016/j.ijpharm.2018.08.060 . .