TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I..Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines.12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4,
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Kovačević, Peđa
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Barišić, Vedrana
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
AU  - Rizwan, Zeeshan
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5631
AB  - Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Naunyn-Schmiedeberg's Archives of Pharmacology
T1  - Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study
DO  - 10.1007/s00210-024-03136-1
ER  - 

@article{
author = "Kovačević, Peđa and Milaković, Dragana and Kovačević, Tijana and Barišić, Vedrana and Dragić, Saša and Zlojutro, Biljana and Miljković, Branislava and Vučićević, Katarina and Rizwan, Zeeshan",
abstract = "Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Naunyn-Schmiedeberg's Archives of Pharmacology",
title = "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study",
doi = "10.1007/s00210-024-03136-1"
}

Kovačević, P., Milaković, D., Kovačević, T., Barišić, V., Dragić, S., Zlojutro, B., Miljković, B., Vučićević, K.,& Rizwan, Z..Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00210-024-03136-1

Kovačević P, Milaković D, Kovačević T, Barišić V, Dragić S, Zlojutro B, Miljković B, Vučićević K, Rizwan Z. Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology..
doi:10.1007/s00210-024-03136-1 .

Kovačević, Peđa, Milaković, Dragana, Kovačević, Tijana, Barišić, Vedrana, Dragić, Saša, Zlojutro, Biljana, Miljković, Branislava, Vučićević, Katarina, Rizwan, Zeeshan, "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study" in Naunyn-Schmiedeberg's Archives of Pharmacology,
https://doi.org/10.1007/s00210-024-03136-1 . .

TY  - JOUR
AU  - Skalny, Anatoly V.
AU  - Aschner, Michael
AU  - Zhang, Feng
AU  - Guo, Xiong
AU  - Buha-Đorđević, Aleksandra
AU  - Sotnikova, Tatiana I.
AU  - Korobeinikova, Tatiana V.
AU  - Domingo, Jose L.
AU  - Farsky, Sandra H. P.
AU  - Tinkov, Alexey A.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5645
AB  - The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Archives of Toxicology
T1  - Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis
DO  - 10.1007/s00204-024-03772-9
ER  - 

@article{
author = "Skalny, Anatoly V. and Aschner, Michael and Zhang, Feng and Guo, Xiong and Buha-Đorđević, Aleksandra and Sotnikova, Tatiana I. and Korobeinikova, Tatiana V. and Domingo, Jose L. and Farsky, Sandra H. P. and Tinkov, Alexey A.",
year = "2024",
abstract = "The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Archives of Toxicology",
title = "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis",
doi = "10.1007/s00204-024-03772-9"
}

Skalny, A. V., Aschner, M., Zhang, F., Guo, X., Buha-Đorđević, A., Sotnikova, T. I., Korobeinikova, T. V., Domingo, J. L., Farsky, S. H. P.,& Tinkov, A. A.. (2024). Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00204-024-03772-9

Skalny AV, Aschner M, Zhang F, Guo X, Buha-Đorđević A, Sotnikova TI, Korobeinikova TV, Domingo JL, Farsky SHP, Tinkov AA. Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology. 2024;.
doi:10.1007/s00204-024-03772-9 .

Skalny, Anatoly V., Aschner, Michael, Zhang, Feng, Guo, Xiong, Buha-Đorđević, Aleksandra, Sotnikova, Tatiana I., Korobeinikova, Tatiana V., Domingo, Jose L., Farsky, Sandra H. P., Tinkov, Alexey A., "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis" in Archives of Toxicology (2024),
https://doi.org/10.1007/s00204-024-03772-9 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Munjas, Jelena
AU  - Kovačević, Smiljka
AU  - Guzonjić, Azra
AU  - Mitrović, Jadranka
AU  - Saso, Luciano
AU  - Đuričić, Ivana
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5654
AB  - Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Childhood obesity accelerates biological ageing: is oxidative stress a link?
DO  - 10.1017/S0007114524000898
ER  - 

@article{
author = "Subošić, Branko and Zdravković, Vera and Ješić, Maja and Munjas, Jelena and Kovačević, Smiljka and Guzonjić, Azra and Mitrović, Jadranka and Saso, Luciano and Đuričić, Ivana and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Obesity is multifactorial pathophysiological condition with an imbalance in biochemical, immunochemical, redox status and genetic parameters values. We aimed estimate connection between relative leukocyte telomere lengths (rLRL) - biomarker of cellular aging with metabolic and redox status biomarkers values in a group of obese and lean children. The study includes 110 obese and 42 lean children and adolescents, both genders. The results suggested that rLTL are significantly shorter in obese, compared to lean group (p<0,01). Negative correlation of rLTL with total oxidant status, TOS (Spearman's ρ = -0.365, p<0.001) as well as with C reactive protein (Spearman's ρ= -0,363, p<0.001) were observed. Principal component analysis (PCA) extracted three distinct factors (i.e. principal components) entitled as: prooxidant factor with 35% of total variability; antioxidant factor with 30% of total variability and lipid antioxidant - biological ageing factor with 12% of the total variability. The most important predictor of body mass index (BMI) >30kg/m2 according to logistic regression analysis was PCA derived antioxidant factor's score (OR: 1.66, 95th Cl: 1.05-2.6, p=0.029). PCA analysis confirmed oxidative stress importance in biological ageing caused by obesity and its multiple consequences related to prooxidants augmentation and antioxidants exhaustion and gave us clear signs of disturbed cellular homeostasis deepness, even before any overt disease occurrence.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Childhood obesity accelerates biological ageing: is oxidative stress a link?",
doi = "10.1017/S0007114524000898"
}

Subošić, B., Zdravković, V., Ješić, M., Munjas, J., Kovačević, S., Guzonjić, A., Mitrović, J., Saso, L., Đuričić, I.,& Kotur-Stevuljević, J.. (2024). Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition
Cambridge University Press..
https://doi.org/10.1017/S0007114524000898

Subošić B, Zdravković V, Ješić M, Munjas J, Kovačević S, Guzonjić A, Mitrović J, Saso L, Đuričić I, Kotur-Stevuljević J. Childhood obesity accelerates biological ageing: is oxidative stress a link?. in British Journal of Nutrition. 2024;.
doi:10.1017/S0007114524000898 .

Subošić, Branko, Zdravković, Vera, Ješić, Maja, Munjas, Jelena, Kovačević, Smiljka, Guzonjić, Azra, Mitrović, Jadranka, Saso, Luciano, Đuričić, Ivana, Kotur-Stevuljević, Jelena, "Childhood obesity accelerates biological ageing: is oxidative stress a link?" in British Journal of Nutrition (2024),
https://doi.org/10.1017/S0007114524000898 . .

TY  - JOUR
AU  - Tomić, Tanja
AU  - Henman, Martin
AU  - Tadić, Ivana
AU  - Antić-Stanković, Jelena
AU  - Santric Milicevic, Milena
AU  - Bukumirić, Zoran
AU  - Lakić, Dragana
AU  - Odalović, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5653
AB  - Introduction: The coronavirus disease 2019 (COVID-19) pandemic affected antibiotic usage worldwide. However, there is limited data from Serbia. Dispensing of oral antibiotics in Serbian pharmacies was analyzed to calculate monthly and yearly changes between 2018-2021, and to explore immediate and long-term effects of COVID-19 on antibiotic dispensing during this period. Methodology: The number of antibiotic packages dispensed from pharmacies during the study period was analyzed with a Chi-square test to assess the average change in annual dispensing, and an interrupted time-series analysis was used to evaluate the impact of the pandemic on antibiotic dispensing. The data from 2018-2021 were retrieved from the database of a large community pharmacy chain in Serbia. Results: The average number of antibiotic packages dispensed per day and per pharmacy was higher in 2021 compared to 2018 by one package. However, the dispensing of macrolides increased significantly; 17.7% (2018) vs. 22.5% (2021) (p < 0.05). In general, an increase in antibiotic dispensing was detected during COVID-19 for total antibiotics (16.4%), Watch antibiotics (44.8%), third-generation cephalosporins (80.4%), macrolides (45.5%) and azithromycin (83.7%). However, the immediate effect of COVID-19 was a decrease in the dispensing of Watch antibiotics, penicillin, and third-generation cephalosporins (p < 0.05); and a notable long-term COVID-19 effect was an increase in the dispensing of azithromycin (p < 0.05). Conclusions: In spite of a relatively stable trend of total antibiotic dispensing before and during COVID-19 pandemic, the use of Watch antibiotics, third-generation cephalosporins, and macrolides (particularly azithromycin) showed an increasing trend in dispensing that should be optimized.
PB  - Journal of Infection in Developing Countries
T2  - Journal of Infection in Developing Countries
T1  - Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics
VL  - 18
IS  - 4
SP  - 504
EP  - 512
DO  - 10.3855/jidc.19255
ER  - 

@article{
author = "Tomić, Tanja and Henman, Martin and Tadić, Ivana and Antić-Stanković, Jelena and Santric Milicevic, Milena and Bukumirić, Zoran and Lakić, Dragana and Odalović, Marina",
year = "2024",
abstract = "Introduction: The coronavirus disease 2019 (COVID-19) pandemic affected antibiotic usage worldwide. However, there is limited data from Serbia. Dispensing of oral antibiotics in Serbian pharmacies was analyzed to calculate monthly and yearly changes between 2018-2021, and to explore immediate and long-term effects of COVID-19 on antibiotic dispensing during this period. Methodology: The number of antibiotic packages dispensed from pharmacies during the study period was analyzed with a Chi-square test to assess the average change in annual dispensing, and an interrupted time-series analysis was used to evaluate the impact of the pandemic on antibiotic dispensing. The data from 2018-2021 were retrieved from the database of a large community pharmacy chain in Serbia. Results: The average number of antibiotic packages dispensed per day and per pharmacy was higher in 2021 compared to 2018 by one package. However, the dispensing of macrolides increased significantly; 17.7% (2018) vs. 22.5% (2021) (p < 0.05). In general, an increase in antibiotic dispensing was detected during COVID-19 for total antibiotics (16.4%), Watch antibiotics (44.8%), third-generation cephalosporins (80.4%), macrolides (45.5%) and azithromycin (83.7%). However, the immediate effect of COVID-19 was a decrease in the dispensing of Watch antibiotics, penicillin, and third-generation cephalosporins (p < 0.05); and a notable long-term COVID-19 effect was an increase in the dispensing of azithromycin (p < 0.05). Conclusions: In spite of a relatively stable trend of total antibiotic dispensing before and during COVID-19 pandemic, the use of Watch antibiotics, third-generation cephalosporins, and macrolides (particularly azithromycin) showed an increasing trend in dispensing that should be optimized.",
publisher = "Journal of Infection in Developing Countries",
journal = "Journal of Infection in Developing Countries",
title = "Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics",
volume = "18",
number = "4",
pages = "504-512",
doi = "10.3855/jidc.19255"
}

Tomić, T., Henman, M., Tadić, I., Antić-Stanković, J., Santric Milicevic, M., Bukumirić, Z., Lakić, D.,& Odalović, M.. (2024). Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics. in Journal of Infection in Developing Countries
Journal of Infection in Developing Countries., 18(4), 504-512.
https://doi.org/10.3855/jidc.19255

Tomić T, Henman M, Tadić I, Antić-Stanković J, Santric Milicevic M, Bukumirić Z, Lakić D, Odalović M. Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics. in Journal of Infection in Developing Countries. 2024;18(4):504-512.
doi:10.3855/jidc.19255 .

Tomić, Tanja, Henman, Martin, Tadić, Ivana, Antić-Stanković, Jelena, Santric Milicevic, Milena, Bukumirić, Zoran, Lakić, Dragana, Odalović, Marina, "Immediate and long-term effects of COVID-19 on antibiotic dispensing: increasing use of Watch antibiotics" in Journal of Infection in Developing Countries, 18, no. 4 (2024):504-512,
https://doi.org/10.3855/jidc.19255 . .

TY  - JOUR
AU  - Vujković, Branko
AU  - Brković, Voin
AU  - Pajičić, Ana
AU  - Pavlović, Vedrana
AU  - Stanisavljević, Dejana
AU  - Krajnović, Dušanka
AU  - Jović Vraneš, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5652
AB  - Background: As digital health services are increasingly developing and becoming more interactive in Serbia, a comprehensive instrument for measuring eHealth literacy (EHL) is needed. Objective: This study aimed to translate, culturally adapt, and investigate the psychometric properties of the Serbian version of the eHealth Literacy Questionnaire (eHLQ); to evaluate EHL in the population of primary health care (PHC) users in Serbia; and to explore factors associated with their EHL. Methods: The validation study was conducted in 8 PHC centers in the territory of the Mačva district in Western Serbia. A stratified sampling method was used to obtain a representative sample. The Translation Integrity Procedure was followed to adapt the questionnaire to the Serbian language. The psychometric properties of the Serbian version of the eHLQ were analyzed through the examination of factorial structure, internal consistency, and test-retest reliability. Descriptive statistics were calculated to determine participant characteristics. Differences between groups were tested by the 2-tailed Students t test and ANOVA. Univariable and multivariable linear regression analyses were used to determine factors related to EHL. Results: A total of 475 PHC users were enrolled. The mean age was 51.0 (SD 17.3; range 19-94) years, and most participants were female (328/475, 69.1%). Confirmatory factor analysis validated the 7-factor structure of the questionnaire. Values for incremental fit index (0.96) and comparative fit index (0.95) were above the cutoff of ≥0.95. The root mean square error of approximation value of 0.05 was below the suggested value of ≤0.06. Cronbach α of the entire scale was 0.95, indicating excellent scale reliability, with Cronbach α ranging from 0.81 to 0.90 for domains. The intraclass correlation coefficient ranged from 0.63 to 0.82, indicating moderate to good test-retest reliability. The highest EHL mean scores were obtained for the understanding of health concepts and language (mean 2.86, SD 0.32) and feel safe and in control (mean 2.89, SD 0.33) domains. Statistically significant differences (all P<.05) for all 7 eHLQ scores were observed for age, education, perceived material status, perceived health status, searching for health information on the internet, and occupation (except domain 4). In multivariable regression models, searching for health information on the internet and being aged younger than 65 years were associated with higher values of all domain scores except the domain feel safe and in control for variable age. Conclusions: This study demonstrates that the Serbian version of the eHLQ can be a useful tool in the measurement of EHL and in the planning of digital health interventions at the population and individual level due to its strong psychometric properties in the Serbian context.
PB  - JMIR Publications Inc.
T2  - Journal of Medical Internet Research
T1  - Serbian Version of the eHealth Literacy Questionnaire (eHLQ): Translation, Cultural Adaptation, and Validation Study Among Primary Health Care Users
VL  - 26
SP  - e57963
DO  - 10.2196/57963
ER  - 

@article{
author = "Vujković, Branko and Brković, Voin and Pajičić, Ana and Pavlović, Vedrana and Stanisavljević, Dejana and Krajnović, Dušanka and Jović Vraneš, Aleksandra",
year = "2024",
abstract = "Background: As digital health services are increasingly developing and becoming more interactive in Serbia, a comprehensive instrument for measuring eHealth literacy (EHL) is needed. Objective: This study aimed to translate, culturally adapt, and investigate the psychometric properties of the Serbian version of the eHealth Literacy Questionnaire (eHLQ); to evaluate EHL in the population of primary health care (PHC) users in Serbia; and to explore factors associated with their EHL. Methods: The validation study was conducted in 8 PHC centers in the territory of the Mačva district in Western Serbia. A stratified sampling method was used to obtain a representative sample. The Translation Integrity Procedure was followed to adapt the questionnaire to the Serbian language. The psychometric properties of the Serbian version of the eHLQ were analyzed through the examination of factorial structure, internal consistency, and test-retest reliability. Descriptive statistics were calculated to determine participant characteristics. Differences between groups were tested by the 2-tailed Students t test and ANOVA. Univariable and multivariable linear regression analyses were used to determine factors related to EHL. Results: A total of 475 PHC users were enrolled. The mean age was 51.0 (SD 17.3; range 19-94) years, and most participants were female (328/475, 69.1%). Confirmatory factor analysis validated the 7-factor structure of the questionnaire. Values for incremental fit index (0.96) and comparative fit index (0.95) were above the cutoff of ≥0.95. The root mean square error of approximation value of 0.05 was below the suggested value of ≤0.06. Cronbach α of the entire scale was 0.95, indicating excellent scale reliability, with Cronbach α ranging from 0.81 to 0.90 for domains. The intraclass correlation coefficient ranged from 0.63 to 0.82, indicating moderate to good test-retest reliability. The highest EHL mean scores were obtained for the understanding of health concepts and language (mean 2.86, SD 0.32) and feel safe and in control (mean 2.89, SD 0.33) domains. Statistically significant differences (all P<.05) for all 7 eHLQ scores were observed for age, education, perceived material status, perceived health status, searching for health information on the internet, and occupation (except domain 4). In multivariable regression models, searching for health information on the internet and being aged younger than 65 years were associated with higher values of all domain scores except the domain feel safe and in control for variable age. Conclusions: This study demonstrates that the Serbian version of the eHLQ can be a useful tool in the measurement of EHL and in the planning of digital health interventions at the population and individual level due to its strong psychometric properties in the Serbian context.",
publisher = "JMIR Publications Inc.",
journal = "Journal of Medical Internet Research",
title = "Serbian Version of the eHealth Literacy Questionnaire (eHLQ): Translation, Cultural Adaptation, and Validation Study Among Primary Health Care Users",
volume = "26",
pages = "e57963",
doi = "10.2196/57963"
}

Vujković, B., Brković, V., Pajičić, A., Pavlović, V., Stanisavljević, D., Krajnović, D.,& Jović Vraneš, A.. (2024). Serbian Version of the eHealth Literacy Questionnaire (eHLQ): Translation, Cultural Adaptation, and Validation Study Among Primary Health Care Users. in Journal of Medical Internet Research
JMIR Publications Inc.., 26, e57963.
https://doi.org/10.2196/57963

Vujković B, Brković V, Pajičić A, Pavlović V, Stanisavljević D, Krajnović D, Jović Vraneš A. Serbian Version of the eHealth Literacy Questionnaire (eHLQ): Translation, Cultural Adaptation, and Validation Study Among Primary Health Care Users. in Journal of Medical Internet Research. 2024;26:e57963.
doi:10.2196/57963 .

Vujković, Branko, Brković, Voin, Pajičić, Ana, Pavlović, Vedrana, Stanisavljević, Dejana, Krajnović, Dušanka, Jović Vraneš, Aleksandra, "Serbian Version of the eHealth Literacy Questionnaire (eHLQ): Translation, Cultural Adaptation, and Validation Study Among Primary Health Care Users" in Journal of Medical Internet Research, 26 (2024):e57963,
https://doi.org/10.2196/57963 . .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Demisli, Sortiria
AU  - Savić, Sanela M.
AU  - Savić, Saša R.
AU  - Randjelović, Danijela V.
AU  - Marković, Bojan
AU  - Pantelić, Ivana
AU  - Mitrović, Jelena
AU  - Stanković, Tijana
AU  - Papadimitrou, Vassiliki
AU  - Xenakis, Aristotelis
AU  - Savić, Snežana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5651
AB  - Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties
VL  - 404
DO  - 10.1016/j.molliq.2024.124888
ER  - 

@article{
author = "Đoković, Jelena and Demisli, Sortiria and Savić, Sanela M. and Savić, Saša R. and Randjelović, Danijela V. and Marković, Bojan and Pantelić, Ivana and Mitrović, Jelena and Stanković, Tijana and Papadimitrou, Vassiliki and Xenakis, Aristotelis and Savić, Snežana",
year = "2024",
abstract = "Nanoemulsions (NEs) are frequently used in the food, cosmetics and pharmaceutical industries to deliver nutraceuticals, pharmaceutical or cosmetic active ingredients. When administering NEs parenterally, various stabilisers are added to prevent rapid plasma clearance and to successfully deliver the active ingredient to the target site. For this purpose, PEGylation is often used to prolong the circulation time of the droplets. However, the problem is to determine the optimal concentration of the PEGylating agent – the PEGylation efficacy – that ensures adequate surface protection. This is a particular challenge when the active ingredient is incorporated into the stabilising layer, where any changes could disrupt the stability of the droplet. For this reason, we aimed to determine the optimal concentration of PEG2000-DSPE for surface protection of curcumin-loaded NEs for parenteral administration using electron paramagnetic resonance (EPR) spectroscopy. NEs were prepared using the high pressure homogenisation technique with 0.1 %, 0.3 % or 0.6 % of the PEGylated phospholipid. A droplet size of approximately 100 nm and polydispersity index below 0.25 indicated suitability for parenteral application. EPR analysis showed that PEG2000-DSPE had a stabilising effect on selected NEs, which was most pronounced in the part of the stabilising layer closest to the aqueous phase. To confirm these results, protein interaction studies were carried out using dynamic light scattering, UV–Vis spectroscopy, atomic force microscopy and release studies from protein-enriched media – bovine serum albumin (BSA) or foetal bovine serum (FBS) in phosphate-buffered saline. These analyses confirmed that the addition of PEG2000-DSPE reduced protein binding to the droplets as a function of concentration, with 0.3 % providing the best protection for the droplets. Our conclusions from the EPR spectroscopy study demonstrate the usefulness of EPR in determining the optimal concentrations of PEGylating agents for surface coverage and its usefulness in the formulation development phase.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties",
volume = "404",
doi = "10.1016/j.molliq.2024.124888"
}

Đoković, J., Demisli, S., Savić, S. M., Savić, S. R., Randjelović, D. V., Marković, B., Pantelić, I., Mitrović, J., Stanković, T., Papadimitrou, V., Xenakis, A.,& Savić, S.. (2024). Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids
Elsevier B.V.., 404.
https://doi.org/10.1016/j.molliq.2024.124888

Đoković J, Demisli S, Savić SM, Savić SR, Randjelović DV, Marković B, Pantelić I, Mitrović J, Stanković T, Papadimitrou V, Xenakis A, Savić S. Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties. in Journal of Molecular Liquids. 2024;404.
doi:10.1016/j.molliq.2024.124888 .

Đoković, Jelena, Demisli, Sortiria, Savić, Sanela M., Savić, Saša R., Randjelović, Danijela V., Marković, Bojan, Pantelić, Ivana, Mitrović, Jelena, Stanković, Tijana, Papadimitrou, Vassiliki, Xenakis, Aristotelis, Savić, Snežana, "Evaluation of PEGylation efficacy of curcumin-loaded nanoemulsions using complementary methods to assess protein interactions and physicochemical properties" in Journal of Molecular Liquids, 404 (2024),
https://doi.org/10.1016/j.molliq.2024.124888 . .

TY  - JOUR
AU  - Bužančić, Iva
AU  - Držaić, Margita
AU  - Kummer, Ingrid
AU  - Ortner Hadžiabdić, Maja
AU  - Brkić, Jovana
AU  - Fialová, Daniela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5621
AB  - Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-024-56780-1, published online 14 March 2024 The original version of this Article contained an error in the Funding section. “All the research work was funded by the EuroAgeism H2020 project (ESR7 project), supported by the European Union research and innovation program under the Grant Agreement of the Marie Skłodowska-Curie Foundation Number MSCF-ITN-764632. Research works of Assoc. Prof. Daniela Fialová, PharmD, Ph.D. and members of her research team were supported by the Grants: InoMed, Reg. No CZ.02.1.01/0.0/0.0/18_069/0010046, the European Horizon 2020 I-CARE4OLD Grant No 965341, START/MED/093 EN.02.2.69/0.0/0.0/19_073/0016935, SVV 260 551 Grant and Cooperatio research program of the Faculty of Pharmacy, Charles University (Research Unit: “Ageing, Polypharmacotherapy and Changes in Therapeutic Value of Drugs in the Aged’’, KSKF-I.), and NETPHARM project CZ.02.01.01/00/22_008/0004607. The funding organizations had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.” now reads: “Publication of this work was supported by the I-CARE4OLD project that has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No. 96534. Views and opinions expressed are however those of the authors only and do not necessarily reflect those of the European Union. Neither the European Union nor the granting authority can be held responsible for them. More information on the I-CARE4OLD project can be found at http://www.icare4old.eu and https://cordis.europa.eu/project/id/965341. Except secondary analyses and works on the publication, data collection and teamwork were funded also by the EuroAgeism H2020 project (ESR7 project), supported by the European Union research and innovation program under the Grant Agreement of the Marie Skłodowska-Curie Foundation Number MSCF-ITN-764632. Research works of Assoc. Prof. Daniela Fialová, PharmD, Ph.D. and members of her research team were supported by grants: START/MED/093 EN.02.2.69/0.0/0.0/19_073/0016935, SVV 260 551, Cooperatio research program of the Faculty of Pharmacy, Charles University (Research Unit: “Ageing, Polypharmacotherapy and Changes in Terapeutic Value of Drugs in the Aged’’, KSKF-I.), and NETPHARM project CZ.02.01.01/00/22_008/0004607.” The original Article has been corrected.
PB  - Nature Research
T2  - Scientific Reports
T1  - Correction to: Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment (Scientific Reports, (2024), 14, 1, (6235), 10.1038/s41598-024-56780-1)
VL  - 14
IS  - 1
DO  - 10.1038/s41598-024-60073-y
ER  - 

@article{
author = "Bužančić, Iva and Držaić, Margita and Kummer, Ingrid and Ortner Hadžiabdić, Maja and Brkić, Jovana and Fialová, Daniela",
year = "2024",
abstract = "Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-024-56780-1, published online 14 March 2024 The original version of this Article contained an error in the Funding section. “All the research work was funded by the EuroAgeism H2020 project (ESR7 project), supported by the European Union research and innovation program under the Grant Agreement of the Marie Skłodowska-Curie Foundation Number MSCF-ITN-764632. Research works of Assoc. Prof. Daniela Fialová, PharmD, Ph.D. and members of her research team were supported by the Grants: InoMed, Reg. No CZ.02.1.01/0.0/0.0/18_069/0010046, the European Horizon 2020 I-CARE4OLD Grant No 965341, START/MED/093 EN.02.2.69/0.0/0.0/19_073/0016935, SVV 260 551 Grant and Cooperatio research program of the Faculty of Pharmacy, Charles University (Research Unit: “Ageing, Polypharmacotherapy and Changes in Therapeutic Value of Drugs in the Aged’’, KSKF-I.), and NETPHARM project CZ.02.01.01/00/22_008/0004607. The funding organizations had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.” now reads: “Publication of this work was supported by the I-CARE4OLD project that has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No. 96534. Views and opinions expressed are however those of the authors only and do not necessarily reflect those of the European Union. Neither the European Union nor the granting authority can be held responsible for them. More information on the I-CARE4OLD project can be found at http://www.icare4old.eu and https://cordis.europa.eu/project/id/965341. Except secondary analyses and works on the publication, data collection and teamwork were funded also by the EuroAgeism H2020 project (ESR7 project), supported by the European Union research and innovation program under the Grant Agreement of the Marie Skłodowska-Curie Foundation Number MSCF-ITN-764632. Research works of Assoc. Prof. Daniela Fialová, PharmD, Ph.D. and members of her research team were supported by grants: START/MED/093 EN.02.2.69/0.0/0.0/19_073/0016935, SVV 260 551, Cooperatio research program of the Faculty of Pharmacy, Charles University (Research Unit: “Ageing, Polypharmacotherapy and Changes in Terapeutic Value of Drugs in the Aged’’, KSKF-I.), and NETPHARM project CZ.02.01.01/00/22_008/0004607.” The original Article has been corrected.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Correction to: Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment (Scientific Reports, (2024), 14, 1, (6235), 10.1038/s41598-024-56780-1)",
volume = "14",
number = "1",
doi = "10.1038/s41598-024-60073-y"
}

Bužančić, I., Držaić, M., Kummer, I., Ortner Hadžiabdić, M., Brkić, J.,& Fialová, D.. (2024). Correction to: Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment (Scientific Reports, (2024), 14, 1, (6235), 10.1038/s41598-024-56780-1). in Scientific Reports
Nature Research., 14(1).
https://doi.org/10.1038/s41598-024-60073-y

Bužančić I, Držaić M, Kummer I, Ortner Hadžiabdić M, Brkić J, Fialová D. Correction to: Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment (Scientific Reports, (2024), 14, 1, (6235), 10.1038/s41598-024-56780-1). in Scientific Reports. 2024;14(1).
doi:10.1038/s41598-024-60073-y .

Bužančić, Iva, Držaić, Margita, Kummer, Ingrid, Ortner Hadžiabdić, Maja, Brkić, Jovana, Fialová, Daniela, "Correction to: Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment (Scientific Reports, (2024), 14, 1, (6235), 10.1038/s41598-024-56780-1)" in Scientific Reports, 14, no. 1 (2024),
https://doi.org/10.1038/s41598-024-60073-y . .

TY  - JOUR
AU  - Bećirović, Sabina
AU  - Vuković, Bojana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5632
AB  - The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.
PB  - Taylor and Francis Ltd.
T2  - Biotechnology and Biotechnological Equipment
T1  - Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina
VL  - 38
IS  - 1
DO  - 10.1080/13102818.2024.2349593
ER  - 

@article{
author = "Bećirović, Sabina and Vuković, Bojana and Marinković, Valentina",
year = "2024",
abstract = "The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.",
publisher = "Taylor and Francis Ltd.",
journal = "Biotechnology and Biotechnological Equipment",
title = "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina",
volume = "38",
number = "1",
doi = "10.1080/13102818.2024.2349593"
}

Bećirović, S., Vuković, B.,& Marinković, V.. (2024). Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment
Taylor and Francis Ltd.., 38(1).
https://doi.org/10.1080/13102818.2024.2349593

Bećirović S, Vuković B, Marinković V. Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment. 2024;38(1).
doi:10.1080/13102818.2024.2349593 .

Bećirović, Sabina, Vuković, Bojana, Marinković, Valentina, "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina" in Biotechnology and Biotechnological Equipment, 38, no. 1 (2024),
https://doi.org/10.1080/13102818.2024.2349593 . .

TY  - JOUR
AU  - Turković, Erna
AU  - Vasiljević, Ivana
AU  - Parojčić, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5650
AB  - Orodispersible films (ODFs) have emerged as innovative pharmaceutical dosage forms, offering patient-specific treatment through adjustable dosing and the combination of diverse active ingredients. This expanding field generates vast datasets, requiring advanced analytical techniques for deeper understanding of data itself. Machine learning is becoming an important tool in the rapidly changing field of pharmaceutical research, particularly in drug preformulation studies. This work aims to explore into the application of machine learning methods for the analysis of experimental data obtained by ODF characterization in order to obtain an insight into the factors governing ODF performance and use it as guidance in pharmaceutical development. Using a dataset derived from extensive experimental studies, various machine learning algorithms were employed to cluster and predict critical properties of ODFs. Our results demonstrate that machine learning models, including Support vector machine, Random forest and Deep learning, exhibit high accuracy in predicting the mechanical properties of ODFs, such as flexibility and rigidity. The predictive models offered insights into the complex interaction of formulation variables. This research is a pilot study that highlights the potential of machine learning as a transformative approach in the pharmaceutical field, paving the way for more efficient and informed drug development processes.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development
VL  - 658
DO  - 10.1016/j.ijpharm.2024.124188
ER  - 

@article{
author = "Turković, Erna and Vasiljević, Ivana and Parojčić, Jelena",
year = "2024",
abstract = "Orodispersible films (ODFs) have emerged as innovative pharmaceutical dosage forms, offering patient-specific treatment through adjustable dosing and the combination of diverse active ingredients. This expanding field generates vast datasets, requiring advanced analytical techniques for deeper understanding of data itself. Machine learning is becoming an important tool in the rapidly changing field of pharmaceutical research, particularly in drug preformulation studies. This work aims to explore into the application of machine learning methods for the analysis of experimental data obtained by ODF characterization in order to obtain an insight into the factors governing ODF performance and use it as guidance in pharmaceutical development. Using a dataset derived from extensive experimental studies, various machine learning algorithms were employed to cluster and predict critical properties of ODFs. Our results demonstrate that machine learning models, including Support vector machine, Random forest and Deep learning, exhibit high accuracy in predicting the mechanical properties of ODFs, such as flexibility and rigidity. The predictive models offered insights into the complex interaction of formulation variables. This research is a pilot study that highlights the potential of machine learning as a transformative approach in the pharmaceutical field, paving the way for more efficient and informed drug development processes.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development",
volume = "658",
doi = "10.1016/j.ijpharm.2024.124188"
}

Turković, E., Vasiljević, I.,& Parojčić, J.. (2024). A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development. in International Journal of Pharmaceutics
Elsevier B.V.., 658.
https://doi.org/10.1016/j.ijpharm.2024.124188

Turković E, Vasiljević I, Parojčić J. A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development. in International Journal of Pharmaceutics. 2024;658.
doi:10.1016/j.ijpharm.2024.124188 .

Turković, Erna, Vasiljević, Ivana, Parojčić, Jelena, "A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development" in International Journal of Pharmaceutics, 658 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124188 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljević, Milica
AU  - Janković, Radmila
AU  - Đuretić, Jasmina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5626
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
PB  - MDPI
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
VL  - 12
IS  - 4
SP  - 717
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Biljana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljević, Milica and Janković, Radmila and Đuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.",
publisher = "MDPI",
journal = "Biomedicines",
title = "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats",
volume = "12",
number = "4",
pages = "717",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljević, M., Janković, R.,& Đuretić, J.. (2024). NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
MDPI., 12(4), 717.
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljević M, Janković R, Đuretić J. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4):717.
doi:10.3390/biomedicines12040717 .

Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024):717,
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Varga, Filip
AU  - Liber, Zlatko
AU  - Turudić, Ante
AU  - Jakše, Jernej
AU  - Juzbašić, Lea
AU  - Jeran, Nina
AU  - Grdiša, Martina
AU  - Zbiljić, Miloš
AU  - Šatović, Zlatko
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5609
AB  - Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution
in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid
species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo
assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum
assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying
different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and
after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles
were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis
of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum
than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and
genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted
exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae
individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis
of genetic diversity and structure, as well as molecular identification of their putative hybrids in
the future.
PB  - MDPI
T2  - Diversity
T1  - Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid
VL  - 16
IS  - 4
SP  - 204
DO  - 10.3390/d16040206
ER  - 

@article{
author = "Varga, Filip and Liber, Zlatko and Turudić, Ante and Jakše, Jernej and Juzbašić, Lea and Jeran, Nina and Grdiša, Martina and Zbiljić, Miloš and Šatović, Zlatko",
year = "2024",
abstract = "Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution
in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid
species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo
assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum
assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying
different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and
after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles
were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis
of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum
than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and
genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted
exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae
individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis
of genetic diversity and structure, as well as molecular identification of their putative hybrids in
the future.",
publisher = "MDPI",
journal = "Diversity",
title = "Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid",
volume = "16",
number = "4",
pages = "204",
doi = "10.3390/d16040206"
}

Varga, F., Liber, Z., Turudić, A., Jakše, J., Juzbašić, L., Jeran, N., Grdiša, M., Zbiljić, M.,& Šatović, Z.. (2024). Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid. in Diversity
MDPI., 16(4), 204.
https://doi.org/10.3390/d16040206

Varga F, Liber Z, Turudić A, Jakše J, Juzbašić L, Jeran N, Grdiša M, Zbiljić M, Šatović Z. Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid. in Diversity. 2024;16(4):204.
doi:10.3390/d16040206 .

Varga, Filip, Liber, Zlatko, Turudić, Ante, Jakše, Jernej, Juzbašić, Lea, Jeran, Nina, Grdiša, Martina, Zbiljić, Miloš, Šatović, Zlatko, "Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid" in Diversity, 16, no. 4 (2024):204,
https://doi.org/10.3390/d16040206 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Milinković Budinčić, Jelena
AU  - Stanić, Dušanka
AU  - Fraj, Jadranka
AU  - Petrović, Lidija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5647
AB  - The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.
PB  - MDPI
T2  - Pharmaceutics
T1  - Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050628
ER  - 

@article{
author = "Đekić, Ljiljana and Milinković Budinčić, Jelena and Stanić, Dušanka and Fraj, Jadranka and Petrović, Lidija",
year = "2024",
abstract = "The microencapsulation of α-tocopherol based on the complex coacervation of low-molecular-weight chitosan (LMWC) and sodium lauryl ether sulphate (SLES) without harmful crosslinkers can provide biocompatible carriers that protect it from photodegradation and air oxidation. In this study, the influence of the microcapsule wall composition on carrier performance, compatibility with a high-water-content vehicle for topical application, and release of α-tocopherol were investigated. Although the absence of aldehyde crosslinkers decreased the encapsulation efficiency of α-tocopherol (~70%), the variation in the LMWC/SLES mass ratio (2:1 or 1:1) had no significant effect on the moisture content and microcapsule size. The prepared microcapsule-loaded carbomer hydrogels were soft semisolids with pseudoplastic flow behavior. The integrity of microcapsules embedded in the hydrogel was confirmed by light microscopy. The microcapsules reduced the pH, apparent viscosity, and hysteresis area of the hydrogels, while increasing their spreading ability on a flat inert surface and dispersion rate in artificial sweat. The in vitro release of α-tocopherol from crosslinker-free microcapsule-loaded hydrogels was diffusion-controlled. The release profile was influenced by the LMWC/SLES mass ratio, apparent viscosity, type of synthetic membrane, and acceptor medium composition. Better data quality for the model-independent analysis was achieved when a cellulose nitrate membrane and ethyl alcohol 60% w/w as acceptor medium were used.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050628"
}

Đekić, L., Milinković Budinčić, J., Stanić, D., Fraj, J.,& Petrović, L.. (2024). Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050628

Đekić L, Milinković Budinčić J, Stanić D, Fraj J, Petrović L. Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050628 .

Đekić, Ljiljana, Milinković Budinčić, Jelena, Stanić, Dušanka, Fraj, Jadranka, Petrović, Lidija, "Carbomer Hydrogels with Microencapsulated α-Tocopherol: Focus on the Biocompatibility of the Microcapsules, Topical Application Attributes, and In Vitro Release Study" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050628 . .

TY  - JOUR
AU  - Pejčić, Zorica
AU  - Topić Vučenović, Valentina
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5649
AB  - Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.
PB  - MDPI
T2  - Pharmaceutics
T1  - Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite
VL  - 16
IS  - 5
DO  - 10.3390/pharmaceutics16050685
ER  - 

@article{
author = "Pejčić, Zorica and Topić Vučenović, Valentina and Miljković, Branislava and Vučićević, Katarina",
year = "2024",
abstract = "Clopidogrel (CLO), a pro-drug for preventing thrombotic events, undergoes rapid absorption and extensive metabolism, with approximately 85–90% converted to an inactive carboxylic acid metabolite (CLO-CA) and the remaining to an active thiol (CLO-TH). Few pharmacokinetic models for the drug and its metabolites exist, with most focusing on CLO-TH. Although CLO-CA is inactive, its predominant (compared to its parent drug and metabolites) presence in plasma underscores the importance of characterizing its formation and pharmacokinetic profile. This study aimed to characterize the process of the absorption of CLO and its conversion to CLO-CA via developing a population pharmacokinetic model. Individual participants’ data from two bioequivalence studies were utilized. Extensive blood samples were collected at predetermined intervals, including 841 concentrations of CLO and 1149 of CLO-CA. A nonlinear, mixed-effects modelling approach using NONMEM® software (v 7.5) was applied. A one-compartment model was chosen for CLO, while a two-compartment proved optimal for CLO-CA. Absorption from the depot compartment was modeled via two transit compartments, incorporating transit rate constants (Ktr). A semi-physiological model explained the first-pass effect of CLO, integrating a liver compartment. The estimated mean transit times (MTTs) for the studies were 0.470 and 0.410 h, respectively. The relative bioavailability for each study’s generic medicine compared to the reference were 1.08 and 0.960, respectively. Based on the estimated parameters, the fractions metabolized to inactive metabolites (FiaM_st1 and FiaM_st2) were determined to be 87.27% and 86.87% for the two studies, respectively. The appropriateness of the final model was confirmed. Our model offers a robust framework for elucidating the pharmacokinetic profiles of CLO and CLO-CA.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite",
volume = "16",
number = "5",
doi = "10.3390/pharmaceutics16050685"
}

Pejčić, Z., Topić Vučenović, V., Miljković, B.,& Vučićević, K.. (2024). Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics
MDPI., 16(5).
https://doi.org/10.3390/pharmaceutics16050685

Pejčić Z, Topić Vučenović V, Miljković B, Vučićević K. Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite. in Pharmaceutics. 2024;16(5).
doi:10.3390/pharmaceutics16050685 .

Pejčić, Zorica, Topić Vučenović, Valentina, Miljković, Branislava, Vučićević, Katarina, "Integrating Clopidogrel’s First-Pass Effect in a Joint Semi-Physiological Population Pharmacokinetic Model of the Drug and Its Inactive Carboxylic Acid Metabolite" in Pharmaceutics, 16, no. 5 (2024),
https://doi.org/10.3390/pharmaceutics16050685 . .

Simić, I., Guzonjić, A., Kotur-Stevuljević, J., Ćeriman Krstić, V., Samardžić, N., Savić Vujović, K.,& Jovanović, D.. (2024). Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines
MDPI., 12(5).
https://doi.org/10.3390/biomedicines12050976

Simić I, Guzonjić A, Kotur-Stevuljević J, Ćeriman Krstić V, Samardžić N, Savić Vujović K, Jovanović D. Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study. in Biomedicines. 2024;12(5).
doi:10.3390/biomedicines12050976 .

Simić, Ivana, Guzonjić, Azra, Kotur-Stevuljević, Jelena, Ćeriman Krstić, Vesna, Samardžić, Natalija, Savić Vujović, Katarina, Jovanović, Dragana, "Correlation of Systemic Inflammation Parameters and Serum
SLFN11 in Small Cell Lung Cancer—A Prospective Pilot Study" in Biomedicines, 12, no. 5 (2024),
https://doi.org/10.3390/biomedicines12050976 . .

TY  - JOUR
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Radan, Milica
AU  - Milivojević, Dušan
AU  - Stević, Tatjana
AU  - Pljevljakušić, Dejan
AU  - Nikodinović-Runić, Jasmina
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5642
AB  - Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii
VL  - 216
DO  - 10.1016/j.indcrop.2024.118762
ER  - 

@article{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Radan, Milica and Milivojević, Dušan and Stević, Tatjana and Pljevljakušić, Dejan and Nikodinović-Runić, Jasmina and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii",
volume = "216",
doi = "10.1016/j.indcrop.2024.118762"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Radan, M., Milivojević, D., Stević, T., Pljevljakušić, D., Nikodinović-Runić, J., Bigović, D., Šavikin, K.,& Filipić, B.. (2024). Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products
Elsevier B.V.., 216.
https://doi.org/10.1016/j.indcrop.2024.118762

Kuzmanović Nedeljković S, Ćujić Nikolić N, Radan M, Milivojević D, Stević T, Pljevljakušić D, Nikodinović-Runić J, Bigović D, Šavikin K, Filipić B. Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products. 2024;216.
doi:10.1016/j.indcrop.2024.118762 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Radan, Milica, Milivojević, Dušan, Stević, Tatjana, Pljevljakušić, Dejan, Nikodinović-Runić, Jasmina, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii" in Industrial Crops and Products, 216 (2024),
https://doi.org/10.1016/j.indcrop.2024.118762 . .

TY  - JOUR
AU  - Andrys, Rudolf
AU  - Monnier, Charline
AU  - Antonijević-Miljaković, Evica
AU  - Mickova, Veronika
AU  - Musilek, Kamil
AU  - Zemanova, Lucie
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5643
AB  - Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.
PB  - Elsevier B.V.
T2  - Talanta
T1  - Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research
VL  - 276
DO  - 10.1016/j.talanta.2024.126263
ER  - 

@article{
author = "Andrys, Rudolf and Monnier, Charline and Antonijević-Miljaković, Evica and Mickova, Veronika and Musilek, Kamil and Zemanova, Lucie",
year = "2024",
abstract = "Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.",
publisher = "Elsevier B.V.",
journal = "Talanta",
title = "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research",
volume = "276",
doi = "10.1016/j.talanta.2024.126263"
}

Andrys, R., Monnier, C., Antonijević-Miljaković, E., Mickova, V., Musilek, K.,& Zemanova, L.. (2024). Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta
Elsevier B.V.., 276.
https://doi.org/10.1016/j.talanta.2024.126263

Andrys R, Monnier C, Antonijević-Miljaković E, Mickova V, Musilek K, Zemanova L. Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta. 2024;276.
doi:10.1016/j.talanta.2024.126263 .

Andrys, Rudolf, Monnier, Charline, Antonijević-Miljaković, Evica, Mickova, Veronika, Musilek, Kamil, Zemanova, Lucie, "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research" in Talanta, 276 (2024),
https://doi.org/10.1016/j.talanta.2024.126263 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Marodi, Marko
AU  - Marković, Bojan
AU  - Tomašič, Tihomir
AU  - Durcik, Martina
AU  - Zidar, Nace
AU  - Mašič, Peterlin L.
AU  - Ilaš, Janez
AU  - Kikelj, Danijel
AU  - Čudina, Olivera
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5644
AB  - DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.
PB  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
T1  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
VL  - 1240
DO  - 10.1016/j.jchromb.2024.124158
ER  - 

@article{
author = "Dobričić, Vladimir and Marodi, Marko and Marković, Bojan and Tomašič, Tihomir and Durcik, Martina and Zidar, Nace and Mašič, Peterlin L. and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2024",
abstract = "DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.",
publisher = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
title = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
volume = "1240",
doi = "10.1016/j.jchromb.2024.124158"
}

Dobričić, V., Marodi, M., Marković, B., Tomašič, T., Durcik, M., Zidar, N., Mašič, P. L., Ilaš, J., Kikelj, D.,& Čudina, O.. (2024). Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis., 1240.
https://doi.org/10.1016/j.jchromb.2024.124158

Dobričić V, Marodi M, Marković B, Tomašič T, Durcik M, Zidar N, Mašič PL, Ilaš J, Kikelj D, Čudina O. Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2024;1240.
doi:10.1016/j.jchromb.2024.124158 .

Dobričić, Vladimir, Marodi, Marko, Marković, Bojan, Tomašič, Tihomir, Durcik, Martina, Zidar, Nace, Mašič, Peterlin L., Ilaš, Janez, Kikelj, Danijel, Čudina, Olivera, "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis" in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1240 (2024),
https://doi.org/10.1016/j.jchromb.2024.124158 . .

TY  - JOUR
AU  - Krmar, Jovana
AU  - Svrkota, Bojana
AU  - Obradović, Darija
AU  - Vlatković, Vladimir
AU  - Lazović, Saša
AU  - Otašević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5634
AB  - Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice.
AB  - Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis
T1  - Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi
VL  - 74
IS  - 2
SP  - 133
EP  - 159
DO  - 10.5937/arhfarm74-49565
ER  - 

@article{
author = "Krmar, Jovana and Svrkota, Bojana and Obradović, Darija and Vlatković, Vladimir and Lazović, Saša and Otašević, Biljana",
year = "2024",
abstract = "Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice., Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis, Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi",
volume = "74",
number = "2",
pages = "133-159",
doi = "10.5937/arhfarm74-49565"
}

Krmar, J., Svrkota, B., Obradović, D., Vlatković, V., Lazović, S.,& Otašević, B.. (2024). Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 133-159.
https://doi.org/10.5937/arhfarm74-49565

Krmar J, Svrkota B, Obradović D, Vlatković V, Lazović S, Otašević B. Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju. 2024;74(2):133-159.
doi:10.5937/arhfarm74-49565 .

Krmar, Jovana, Svrkota, Bojana, Obradović, Darija, Vlatković, Vladimir, Lazović, Saša, Otašević, Biljana, "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis" in Arhiv za farmaciju, 74, no. 2 (2024):133-159,
https://doi.org/10.5937/arhfarm74-49565 . .

TY  - JOUR
AU  - Đajić, Nevena
AU  - Krmar, Jovana
AU  - Stojanović, Jevrem
AU  - Svrkota, Bojana
AU  - Otašević, Biljana
AU  - Malenović, Anđelija
AU  - Protić, Ana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5640
AB  - Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion.
AB  - U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products
T1  - 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda
VL  - 74
IS  - 2
SP  - 235
EP  - 266
DO  - 10.5937/arhfarm74-49572
ER  - 

@article{
author = "Đajić, Nevena and Krmar, Jovana and Stojanović, Jevrem and Svrkota, Bojana and Otašević, Biljana and Malenović, Anđelija and Protić, Ana",
year = "2024",
abstract = "Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion., U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products, 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda",
volume = "74",
number = "2",
pages = "235-266",
doi = "10.5937/arhfarm74-49572"
}

Đajić, N., Krmar, J., Stojanović, J., Svrkota, B., Otašević, B., Malenović, A.,& Protić, A.. (2024). Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 235-266.
https://doi.org/10.5937/arhfarm74-49572

Đajić N, Krmar J, Stojanović J, Svrkota B, Otašević B, Malenović A, Protić A. Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju. 2024;74(2):235-266.
doi:10.5937/arhfarm74-49572 .

Đajić, Nevena, Krmar, Jovana, Stojanović, Jevrem, Svrkota, Bojana, Otašević, Biljana, Malenović, Anđelija, Protić, Ana, "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products" in Arhiv za farmaciju, 74, no. 2 (2024):235-266,
https://doi.org/10.5937/arhfarm74-49572 . .

TY  - JOUR
AU  - Ravnikar, Matjaž
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
AU  - Sirše, Mateja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5641
AB  - The present study screened various fungal species for inhibitors of alpha-glucosidase, alpha-amylase, and DPP-4, enzymes that are crucial in carbohydrate metabolism. Ethanolic extracts exhibited superior inhibitory activity compared to water extracts, suggesting their potential as sources of anti-diabetic agents. Further fractionation revealed fomentariol from Fomes fomentarius as a potent inhibitor of alpha-glucosidase and DPP-4, with higher activity against alpha glucosidase than acarbose. Fomentariol presents a novel avenue for diabetes management, demonstrating the simultaneous inhibition of key enzymes in glucose metabolism. However, comprehensive clinical studies are needed to evaluate its safety and efficacy in humans.
PB  - MDPI
T2  - Nutraceuticals
T1  - Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis
VL  - 4
IS  - 2
SP  - 273
EP  - 282
DO  - 10.3390/nutraceuticals4020017
ER  - 

@article{
author = "Ravnikar, Matjaž and Štrukelj, Borut and Otašević, Biljana and Sirše, Mateja",
year = "2024",
abstract = "The present study screened various fungal species for inhibitors of alpha-glucosidase, alpha-amylase, and DPP-4, enzymes that are crucial in carbohydrate metabolism. Ethanolic extracts exhibited superior inhibitory activity compared to water extracts, suggesting their potential as sources of anti-diabetic agents. Further fractionation revealed fomentariol from Fomes fomentarius as a potent inhibitor of alpha-glucosidase and DPP-4, with higher activity against alpha glucosidase than acarbose. Fomentariol presents a novel avenue for diabetes management, demonstrating the simultaneous inhibition of key enzymes in glucose metabolism. However, comprehensive clinical studies are needed to evaluate its safety and efficacy in humans.",
publisher = "MDPI",
journal = "Nutraceuticals",
title = "Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis",
volume = "4",
number = "2",
pages = "273-282",
doi = "10.3390/nutraceuticals4020017"
}

Ravnikar, M., Štrukelj, B., Otašević, B.,& Sirše, M.. (2024). Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis. in Nutraceuticals
MDPI., 4(2), 273-282.
https://doi.org/10.3390/nutraceuticals4020017

Ravnikar M, Štrukelj B, Otašević B, Sirše M. Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis. in Nutraceuticals. 2024;4(2):273-282.
doi:10.3390/nutraceuticals4020017 .

Ravnikar, Matjaž, Štrukelj, Borut, Otašević, Biljana, Sirše, Mateja, "Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis" in Nutraceuticals, 4, no. 2 (2024):273-282,
https://doi.org/10.3390/nutraceuticals4020017 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Vladimirov, Sandra
AU  - Munjas, Jelena
AU  - Mitić, Tijana
AU  - Hall, Ignacio Fernando
AU  - Jušić, Amela
AU  - Ružić, Dušan
AU  - Devaux, Yvan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5637
AB  - Noncoding RNAs (ncRNAs) are pivotal for various pathological processes, impacting disease progression. The potential for leveraging ncRNAs to prevent or treat atherosclerosis and associated cardiovascular diseases is of great significance, especially given the increasing prevalence of atherosclerosis in an ageing and sedentary population. Together, these diseases impose a substantial socio-economic burden, demanding innovative therapeutic solutions. This review explores the potential of ncRNAs in atherosclerosis treatment. We commence by examining approaches for identifying and characterizing atherosclerosis-associated ncRNAs. We then delve into the functional aspects of ncRNAs in atherosclerosis development and progression. Additionally, we review current RNA and RNA-targeting molecules in development or under approval for clinical use, offering insights into their pharmacological potential. The importance of improved ncRNA delivery strategies is highlighted. Finally, we suggest avenues for advanced research to accelerate the use of ncRNAs in treating atherosclerosis and mitigating its societal impact.
PB  - John Wiley and Sons Inc
T2  - British Journal of Pharmacology
T1  - Targeting noncoding RNAs to treat atherosclerosis
DO  - 10.1111/bph.16412
ER  - 

@article{
author = "Sopić, Miron and Vladimirov, Sandra and Munjas, Jelena and Mitić, Tijana and Hall, Ignacio Fernando and Jušić, Amela and Ružić, Dušan and Devaux, Yvan",
year = "2024",
abstract = "Noncoding RNAs (ncRNAs) are pivotal for various pathological processes, impacting disease progression. The potential for leveraging ncRNAs to prevent or treat atherosclerosis and associated cardiovascular diseases is of great significance, especially given the increasing prevalence of atherosclerosis in an ageing and sedentary population. Together, these diseases impose a substantial socio-economic burden, demanding innovative therapeutic solutions. This review explores the potential of ncRNAs in atherosclerosis treatment. We commence by examining approaches for identifying and characterizing atherosclerosis-associated ncRNAs. We then delve into the functional aspects of ncRNAs in atherosclerosis development and progression. Additionally, we review current RNA and RNA-targeting molecules in development or under approval for clinical use, offering insights into their pharmacological potential. The importance of improved ncRNA delivery strategies is highlighted. Finally, we suggest avenues for advanced research to accelerate the use of ncRNAs in treating atherosclerosis and mitigating its societal impact.",
publisher = "John Wiley and Sons Inc",
journal = "British Journal of Pharmacology",
title = "Targeting noncoding RNAs to treat atherosclerosis",
doi = "10.1111/bph.16412"
}

Sopić, M., Vladimirov, S., Munjas, J., Mitić, T., Hall, I. F., Jušić, A., Ružić, D.,& Devaux, Y.. (2024). Targeting noncoding RNAs to treat atherosclerosis. in British Journal of Pharmacology
John Wiley and Sons Inc..
https://doi.org/10.1111/bph.16412

Sopić M, Vladimirov S, Munjas J, Mitić T, Hall IF, Jušić A, Ružić D, Devaux Y. Targeting noncoding RNAs to treat atherosclerosis. in British Journal of Pharmacology. 2024;.
doi:10.1111/bph.16412 .

Sopić, Miron, Vladimirov, Sandra, Munjas, Jelena, Mitić, Tijana, Hall, Ignacio Fernando, Jušić, Amela, Ružić, Dušan, Devaux, Yvan, "Targeting noncoding RNAs to treat atherosclerosis" in British Journal of Pharmacology (2024),
https://doi.org/10.1111/bph.16412 . .

TY  - JOUR
AU  - Samardžić, Stevan
AU  - Đorđić Crnogorac, Marija
AU  - Petković, Miloš
AU  - Arsenijević, Jelena
AU  - Stanojković, Tatjana
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5636
AB  - This study employed the MTT assay to assess the cytotoxicity of one flavan and two stilbene derivatives isolated from the false indigo-bush (Amorpha fruticosa L.) fruits: 5,7-dihydroxy-8-geranylflavanone (1), 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (2), and 2-carboxy-3-hydroxy-
4-prenyl-5-methoxybibenzyl (3). The examined compounds reduced the survival of human cervical and colon tumour cells (HeLa, HT-29, HCT-116, and LS174) with IC50 values ranging from 10.55 to 147.09 μg/mL, except for 1, which did not affect LS174 cells within the tested concentrations. The highest activity was observed for 1 against HeLa cells, and 1 also exhibited the weakest effect against normal foetal lung fibroblasts (IC50 = 166.11 μg/mL), demonstrating good potency and selectivity. Stilbenes 2 and 3 proved efficacious, but lacked selectivity compared to 1. Our findings revealed the cytotoxicity of false indigo-bush constituents, justifying further mechanistic and in vivo investigations, particularly on 5,7-dihydroxy-8-geranylflavanone, which displayed considerable in vitro anticancer capacity and a potentially favourable safety profile.
PB  - Taylor & Francis Ltd.
T2  - Natural Product Research
T1  - Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)
DO  - 10.1080/14786419.2024.2353912
ER  - 

@article{
author = "Samardžić, Stevan and Đorđić Crnogorac, Marija and Petković, Miloš and Arsenijević, Jelena and Stanojković, Tatjana and Maksimović, Zoran",
year = "2024",
abstract = "This study employed the MTT assay to assess the cytotoxicity of one flavan and two stilbene derivatives isolated from the false indigo-bush (Amorpha fruticosa L.) fruits: 5,7-dihydroxy-8-geranylflavanone (1), 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (2), and 2-carboxy-3-hydroxy-
4-prenyl-5-methoxybibenzyl (3). The examined compounds reduced the survival of human cervical and colon tumour cells (HeLa, HT-29, HCT-116, and LS174) with IC50 values ranging from 10.55 to 147.09 μg/mL, except for 1, which did not affect LS174 cells within the tested concentrations. The highest activity was observed for 1 against HeLa cells, and 1 also exhibited the weakest effect against normal foetal lung fibroblasts (IC50 = 166.11 μg/mL), demonstrating good potency and selectivity. Stilbenes 2 and 3 proved efficacious, but lacked selectivity compared to 1. Our findings revealed the cytotoxicity of false indigo-bush constituents, justifying further mechanistic and in vivo investigations, particularly on 5,7-dihydroxy-8-geranylflavanone, which displayed considerable in vitro anticancer capacity and a potentially favourable safety profile.",
publisher = "Taylor & Francis Ltd.",
journal = "Natural Product Research",
title = "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)",
doi = "10.1080/14786419.2024.2353912"
}

Samardžić, S., Đorđić Crnogorac, M., Petković, M., Arsenijević, J., Stanojković, T.,& Maksimović, Z.. (2024). Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in Natural Product Research
Taylor & Francis Ltd...
https://doi.org/10.1080/14786419.2024.2353912

Samardžić S, Đorđić Crnogorac M, Petković M, Arsenijević J, Stanojković T, Maksimović Z. Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in Natural Product Research. 2024;.
doi:10.1080/14786419.2024.2353912 .

Samardžić, Stevan, Đorđić Crnogorac, Marija, Petković, Miloš, Arsenijević, Jelena, Stanojković, Tatjana, Maksimović, Zoran, "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)" in Natural Product Research (2024),
https://doi.org/10.1080/14786419.2024.2353912 . .

TY  - JOUR
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Kovačević, Peđa
AU  - Barišić, Vedrana
AU  - Avram, Sanja
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Momčičević, Danica
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5557
AB  - During veno-venous extracorporeal membrane oxygenation (vv ECMO) therapy, antimicrobial drugs are frequently used, and appropriate dosing is challenging due to there being limited data to support the dosage. Linezolid is effective against multidrug-resistant Gram-positive pathogens frequently isolated in ECMO patients. In total, 53 steady-state linezolid levels were obtained following 600 mg intravenous (IV) injections every 8 h, and these were used to develop a population pharmacokinetic (PopPK) model in patients with COVID-19-associated acute respiratory distress syndrome (CARDS) on vv ECMO. The data were analyzed using a nonlinear mixed-effects modelling approach. Monte Carlo simulation generated 5000 patients’ individual PK parameters and corresponding concentration–time profiles using the PopPK model, following the administration of 600 mg/8 h (a higher-than-standard dosing) and 600 mg/12 h (standard). The probabilities of pharmacokinetic/pharmacodynamic (PK/PD) target attainment (PTA) and the cumulative fraction of responses (CFR) for three pathogens were calculated and compared between the two dosing scenarios. Linezolid 600 mg/8 h was predicted to achieve greater than or equal to 85%Tf>MIC in at least 90% of the patients with CARDS on vv ECMO compared to only approximately two thirds of the patients after dosing every 12 h at a minimal inhibitory concentration (MIC) of 2 mg/L. In addition, for the same MIC, fAUC24/MIC ≥ 80 was achieved in almost three times the number of patients following an 8-h versus a 12-h interval. PopPK simulation predicted that a significantly higher proportion of the patients with CARDS on vv ECMO would achieve the PK/PD targets following the 8-h dosing interval compared to standard linezolid dosing. Nevertheless, the safety concern, in particular, for thrombocytopenia, with higher-than-standard linezolid dosage is reasonable, and consequently, monitoring is essential.
PB  - MDPI
T2  - Pharmaceutics
T1  - Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing
VL  - 16
IS  - 2
DO  - 10.3390/pharmaceutics16020253
ER  - 

@article{
author = "Milaković, Dragana and Kovačević, Tijana and Kovačević, Peđa and Barišić, Vedrana and Avram, Sanja and Dragić, Saša and Zlojutro, Biljana and Momčičević, Danica and Miljković, Branislava and Vučićević, Katarina",
year = "2024",
abstract = "During veno-venous extracorporeal membrane oxygenation (vv ECMO) therapy, antimicrobial drugs are frequently used, and appropriate dosing is challenging due to there being limited data to support the dosage. Linezolid is effective against multidrug-resistant Gram-positive pathogens frequently isolated in ECMO patients. In total, 53 steady-state linezolid levels were obtained following 600 mg intravenous (IV) injections every 8 h, and these were used to develop a population pharmacokinetic (PopPK) model in patients with COVID-19-associated acute respiratory distress syndrome (CARDS) on vv ECMO. The data were analyzed using a nonlinear mixed-effects modelling approach. Monte Carlo simulation generated 5000 patients’ individual PK parameters and corresponding concentration–time profiles using the PopPK model, following the administration of 600 mg/8 h (a higher-than-standard dosing) and 600 mg/12 h (standard). The probabilities of pharmacokinetic/pharmacodynamic (PK/PD) target attainment (PTA) and the cumulative fraction of responses (CFR) for three pathogens were calculated and compared between the two dosing scenarios. Linezolid 600 mg/8 h was predicted to achieve greater than or equal to 85%Tf>MIC in at least 90% of the patients with CARDS on vv ECMO compared to only approximately two thirds of the patients after dosing every 12 h at a minimal inhibitory concentration (MIC) of 2 mg/L. In addition, for the same MIC, fAUC24/MIC ≥ 80 was achieved in almost three times the number of patients following an 8-h versus a 12-h interval. PopPK simulation predicted that a significantly higher proportion of the patients with CARDS on vv ECMO would achieve the PK/PD targets following the 8-h dosing interval compared to standard linezolid dosing. Nevertheless, the safety concern, in particular, for thrombocytopenia, with higher-than-standard linezolid dosage is reasonable, and consequently, monitoring is essential.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing",
volume = "16",
number = "2",
doi = "10.3390/pharmaceutics16020253"
}

Milaković, D., Kovačević, T., Kovačević, P., Barišić, V., Avram, S., Dragić, S., Zlojutro, B., Momčičević, D., Miljković, B.,& Vučićević, K.. (2024). Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing. in Pharmaceutics
MDPI., 16(2).
https://doi.org/10.3390/pharmaceutics16020253

Milaković D, Kovačević T, Kovačević P, Barišić V, Avram S, Dragić S, Zlojutro B, Momčičević D, Miljković B, Vučićević K. Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing. in Pharmaceutics. 2024;16(2).
doi:10.3390/pharmaceutics16020253 .

Milaković, Dragana, Kovačević, Tijana, Kovačević, Peđa, Barišić, Vedrana, Avram, Sanja, Dragić, Saša, Zlojutro, Biljana, Momčičević, Danica, Miljković, Branislava, Vučićević, Katarina, "Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing" in Pharmaceutics, 16, no. 2 (2024),
https://doi.org/10.3390/pharmaceutics16020253 . .

TY  - JOUR
AU  - Vasilić, Đorđe
AU  - Rašević, Marija
AU  - Zečević, Mira
AU  - Čarapić, Marija
AU  - Malenović, Anđelija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5633
AB  - In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis.
AB  - Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis
T1  - Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova
VL  - 74
IS  - 2
SP  - 160
EP  - 177
DO  - 10.5937/arhfarm74-49545
ER  - 

@article{
author = "Vasilić, Đorđe and Rašević, Marija and Zečević, Mira and Čarapić, Marija and Malenović, Anđelija",
year = "2024",
abstract = "In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis., Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis, Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova",
volume = "74",
number = "2",
pages = "160-177",
doi = "10.5937/arhfarm74-49545"
}

Vasilić, Đ., Rašević, M., Zečević, M., Čarapić, M.,& Malenović, A.. (2024). Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 160-177.
https://doi.org/10.5937/arhfarm74-49545

Vasilić Đ, Rašević M, Zečević M, Čarapić M, Malenović A. Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju. 2024;74(2):160-177.
doi:10.5937/arhfarm74-49545 .

Vasilić, Đorđe, Rašević, Marija, Zečević, Mira, Čarapić, Marija, Malenović, Anđelija, "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis" in Arhiv za farmaciju, 74, no. 2 (2024):160-177,
https://doi.org/10.5937/arhfarm74-49545 . .