TY  - JOUR
AU  - Kovačević, Peđa
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Barišić, Vedrana
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
AU  - Rizwan, Zeeshan
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5631
AB  - Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Naunyn-Schmiedeberg's Archives of Pharmacology
T1  - Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study
DO  - 10.1007/s00210-024-03136-1
ER  - 

@article{
author = "Kovačević, Peđa and Milaković, Dragana and Kovačević, Tijana and Barišić, Vedrana and Dragić, Saša and Zlojutro, Biljana and Miljković, Branislava and Vučićević, Katarina and Rizwan, Zeeshan",
abstract = "Patients treated with ECMO are at great risk of nosocomial infections, and around 10% of isolates are gram-positive pathogens. Linezolid (LZD) is effective in the treatment of these infections but appropriate dosing is challenging. The aim was to evaluate the occurrence of thrombocytopenia during ECMO when treated with LZD. An LZD trough concentration of 8 mg/L was set as the cutoff value for thrombocytopenia occurrence among critically ill patients who received parenteral LZD therapy at a dose of 600 mg every 8 h during ECMO. Eleven patients were included in this prospective observational study. Median LZD trough concentrations were 7.85 (interquartile range (IQR), 1.95-11) mg/L. Thrombocytopenia was found in 81.8% of patients. Based on the median LZD trough concentrations cutoff value, patients were divided into two groups, 1.95 (IQR, 0.91–3.6) and 10.3 (IQR, 9.7–11.7) mg/L, respectively. Median platelet values differed significantly between groups on admission, ECMO day 0, ECMO day 1, and LZD sampling day [194 and 152.5, (p < 0.05)], [113 and 214, (p < 0.05)], [76 and 147.5, (p < 0.01)], and [26 and 96.5, (p < 0.01)], respectively. Duration of LZD therapy was similar between the groups. Significant platelet reduction was observed in both groups, emphasizing the need for closer monitoring to prevent LZD-associated thrombocytopenia.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Naunyn-Schmiedeberg's Archives of Pharmacology",
title = "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study",
doi = "10.1007/s00210-024-03136-1"
}

Kovačević, P., Milaković, D., Kovačević, T., Barišić, V., Dragić, S., Zlojutro, B., Miljković, B., Vučićević, K.,& Rizwan, Z..Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00210-024-03136-1

Kovačević P, Milaković D, Kovačević T, Barišić V, Dragić S, Zlojutro B, Miljković B, Vučićević K, Rizwan Z. Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study. in Naunyn-Schmiedeberg's Archives of Pharmacology..
doi:10.1007/s00210-024-03136-1 .

Kovačević, Peđa, Milaković, Dragana, Kovačević, Tijana, Barišić, Vedrana, Dragić, Saša, Zlojutro, Biljana, Miljković, Branislava, Vučićević, Katarina, Rizwan, Zeeshan, "Thrombocytopenia risks in ARDS COVID-19 patients treated with high-dose linezolid during vvECMO therapy: an observational study" in Naunyn-Schmiedeberg's Archives of Pharmacology,
https://doi.org/10.1007/s00210-024-03136-1 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Zdravković, Vera
AU  - Ješić, Maja
AU  - Kovačević, Smiljka
AU  - Đuričić, Ivana
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5625
AB  - Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.
PB  - MDPI
T2  - Biomedicines
T1  - Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity
VL  - 12
IS  - 4
DO  - 10.3390/biomedicines12040883
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša and Zdravković, Vera and Ješić, Maja and Kovačević, Smiljka and Đuričić, Ivana",
abstract = "Fatty acids play a crucial role in obesity development and in the comorbidities of obesity in both adults and children. This study aimed to assess the impact of circulating fatty acids on biomarkers of metabolic health of adolescents living with obesity. Parameters such as blood lipids, redox status, and leukocyte telomere length (rLTL) were measured alongside the proportions of individual fatty acids. The Mann–Whitney U test revealed that individuals with obesity exhibited an unfavorable lipid and redox status compared to the control normal weight group. The group with obesity also had lower plasma n-3 polyunsaturated fatty acids (PUFAs) and a higher ratio of n-6 to n-3 PUFAs than the control group. They also had a shorter rLTL, indicating accelerated biological aging. There was an inverse association of rLTL and plasma n-6-to-n-3 PUFA ratio. Future studies should explore the impact of recommended nutrition plans and increased physical activity on these parameters to determine if these interventions can enhance the health and well-being of adolescents with obesity, knowing that early obesity can track into adulthood.",
publisher = "MDPI",
journal = "Biomedicines",
title = "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity",
volume = "12",
number = "4",
doi = "10.3390/biomedicines12040883"
}

Subošić, B., Kotur-Stevuljević, J., Bogavac-Stanojević, N., Zdravković, V., Ješić, M., Kovačević, S.,& Đuričić, I..Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines
MDPI., 12(4).
https://doi.org/10.3390/biomedicines12040883

Subošić B, Kotur-Stevuljević J, Bogavac-Stanojević N, Zdravković V, Ješić M, Kovačević S, Đuričić I. Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity. in Biomedicines.12(4).
doi:10.3390/biomedicines12040883 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, Zdravković, Vera, Ješić, Maja, Kovačević, Smiljka, Đuričić, Ivana, "Circulating Fatty Acids Associate with Metabolic Changes in Adolescents Living with Obesity" in Biomedicines, 12, no. 4,
https://doi.org/10.3390/biomedicines12040883 . .

TY  - JOUR
AU  - Skalny, Anatoly V.
AU  - Aschner, Michael
AU  - Zhang, Feng
AU  - Guo, Xiong
AU  - Buha-Đorđević, Aleksandra
AU  - Sotnikova, Tatiana I.
AU  - Korobeinikova, Tatiana V.
AU  - Domingo, Jose L.
AU  - Farsky, Sandra H. P.
AU  - Tinkov, Alexey A.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5645
AB  - The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Archives of Toxicology
T1  - Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis
DO  - 10.1007/s00204-024-03772-9
ER  - 

@article{
author = "Skalny, Anatoly V. and Aschner, Michael and Zhang, Feng and Guo, Xiong and Buha-Đorđević, Aleksandra and Sotnikova, Tatiana I. and Korobeinikova, Tatiana V. and Domingo, Jose L. and Farsky, Sandra H. P. and Tinkov, Alexey A.",
year = "2024",
abstract = "The objective of the present study was to review the molecular mechanisms of the adverse effects of environmental pollutants on chondrocytes and extracellular matrix (ECM). Existing data demonstrate that both heavy metals, including cadmium (Cd), lead (Pb), and arsenic (As), as well as organic pollutants, including polychlorinated dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCB), bisphenol A, phthalates, polycyclic aromatic hydrocarbons (PAH), pesticides, and certain other organic pollutants that target cartilage ontogeny and functioning. Overall, environmental pollutants reduce chondrocyte viability through the induction apoptosis, senescence, and inflammatory response, resulting in cell death and impaired ECM production. The effects of organic pollutants on chondrocyte development and viability were shown to be mediated by binding to the aryl hydrocarbon receptor (AhR) signaling and modulation of non-coding RNA expression. Adverse effects of pollutant exposures were observed in articular and growth plate chondrocytes. These mechanisms also damage chondrocyte precursors and subsequently hinder cartilage development. In addition, pollutant exposure was shown to impair chondrogenesis by inhibiting the expression of Sox9 and other regulators. Along with altered Runx2 signaling, these effects also contribute to impaired chondrocyte hypertrophy and chondrocyte-to-osteoblast trans-differentiation, resulting in altered endochondral ossification. Several organic pollutants including PCDD/Fs, PCBs and PAHs, were shown to induce transgenerational adverse effects on cartilage development and the resulting skeletal deformities. Despite of epidemiological evidence linking human environmental pollutant exposure to osteoarthritis or other cartilage pathologies, the data on the molecular mechanisms of adverse effects of environmental pollutant exposure on cartilage tissue were obtained from studies in laboratory rodents, fish, or cell cultures and should be carefully extrapolated to humans, although they clearly demonstrate that cartilage should be considered a putative target for environmental pollutant toxicity.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Archives of Toxicology",
title = "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis",
doi = "10.1007/s00204-024-03772-9"
}

Skalny, A. V., Aschner, M., Zhang, F., Guo, X., Buha-Đorđević, A., Sotnikova, T. I., Korobeinikova, T. V., Domingo, J. L., Farsky, S. H. P.,& Tinkov, A. A.. (2024). Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00204-024-03772-9

Skalny AV, Aschner M, Zhang F, Guo X, Buha-Đorđević A, Sotnikova TI, Korobeinikova TV, Domingo JL, Farsky SHP, Tinkov AA. Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis. in Archives of Toxicology. 2024;.
doi:10.1007/s00204-024-03772-9 .

Skalny, Anatoly V., Aschner, Michael, Zhang, Feng, Guo, Xiong, Buha-Đorđević, Aleksandra, Sotnikova, Tatiana I., Korobeinikova, Tatiana V., Domingo, Jose L., Farsky, Sandra H. P., Tinkov, Alexey A., "Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis" in Archives of Toxicology (2024),
https://doi.org/10.1007/s00204-024-03772-9 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Marodi, Marko
AU  - Marković, Bojan
AU  - Tomašič, Tihomir
AU  - Durcik, Martina
AU  - Zidar, Nace
AU  - Mašič, Peterlin L.
AU  - Ilaš, Janez
AU  - Kikelj, Danijel
AU  - Čudina, Olivera
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5644
AB  - DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.
PB  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
T1  - Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis
VL  - 1240
DO  - 10.1016/j.jchromb.2024.124158
ER  - 

@article{
author = "Dobričić, Vladimir and Marodi, Marko and Marković, Bojan and Tomašič, Tihomir and Durcik, Martina and Zidar, Nace and Mašič, Peterlin L. and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2024",
abstract = "DNA gyrase and topoisomerase IV play significant role in maintaining the correct structure of DNA during replication and they have been identified as validated targets in antibacterial drug discovery. Inadequate pharmacokinetic properties are responsible for many failures during drug discovery and their estimation in the early phase of this process maximizes the chance of getting useful drug candidates. Passive gastrointestinal absorption of a selected group of thirteen dual DNA gyrase and topoisomerase IV inhibitors was estimated using two in vitro tests – parallel artificial membrane permeability assay (PAMPA) and biopartitioning micellar chromatography (BMC). Due to good correlation between obtained results, passive gastrointestinal absorption of remaining ten compounds was estimated using only BMC. With this experimental setup, it was possible to identify compounds with high values of retention factors (k) and highest expected passive gastrointestinal absorption, and compounds with low values of k for which low passive gastrointestinal absorption is predicted. Quantitative structure-retention relationship (QSRR) modelling was performed by creating multiple linear regression (MLR), partial least squares (PLS) and support vector machines (SVM) models. Descriptors with the highest influence on retention factor were identified and their interpretation can be used for the design of new compounds with improved passive gastrointestinal absorption.",
publisher = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
title = "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis",
volume = "1240",
doi = "10.1016/j.jchromb.2024.124158"
}

Dobričić, V., Marodi, M., Marković, B., Tomašič, T., Durcik, M., Zidar, N., Mašič, P. L., Ilaš, J., Kikelj, D.,& Čudina, O.. (2024). Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis., 1240.
https://doi.org/10.1016/j.jchromb.2024.124158

Dobričić V, Marodi M, Marković B, Tomašič T, Durcik M, Zidar N, Mašič PL, Ilaš J, Kikelj D, Čudina O. Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2024;1240.
doi:10.1016/j.jchromb.2024.124158 .

Dobričić, Vladimir, Marodi, Marko, Marković, Bojan, Tomašič, Tihomir, Durcik, Martina, Zidar, Nace, Mašič, Peterlin L., Ilaš, Janez, Kikelj, Danijel, Čudina, Olivera, "Estimation of passive gastrointestinal absorption of new dual DNA gyrase and topoisomerase IV inhibitors using PAMPA and biopartitioning micellar chromatography and quantitative structure-retention relationship analysis" in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1240 (2024),
https://doi.org/10.1016/j.jchromb.2024.124158 . .

TY  - JOUR
AU  - Andrys, Rudolf
AU  - Monnier, Charline
AU  - Antonijević-Miljaković, Evica
AU  - Mickova, Veronika
AU  - Musilek, Kamil
AU  - Zemanova, Lucie
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5643
AB  - Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.
PB  - Elsevier B.V.
T2  - Talanta
T1  - Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research
VL  - 276
DO  - 10.1016/j.talanta.2024.126263
ER  - 

@article{
author = "Andrys, Rudolf and Monnier, Charline and Antonijević-Miljaković, Evica and Mickova, Veronika and Musilek, Kamil and Zemanova, Lucie",
year = "2024",
abstract = "Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.",
publisher = "Elsevier B.V.",
journal = "Talanta",
title = "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research",
volume = "276",
doi = "10.1016/j.talanta.2024.126263"
}

Andrys, R., Monnier, C., Antonijević-Miljaković, E., Mickova, V., Musilek, K.,& Zemanova, L.. (2024). Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta
Elsevier B.V.., 276.
https://doi.org/10.1016/j.talanta.2024.126263

Andrys R, Monnier C, Antonijević-Miljaković E, Mickova V, Musilek K, Zemanova L. Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta. 2024;276.
doi:10.1016/j.talanta.2024.126263 .

Andrys, Rudolf, Monnier, Charline, Antonijević-Miljaković, Evica, Mickova, Veronika, Musilek, Kamil, Zemanova, Lucie, "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research" in Talanta, 276 (2024),
https://doi.org/10.1016/j.talanta.2024.126263 . .

TY  - JOUR
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Radan, Milica
AU  - Milivojević, Dušan
AU  - Stević, Tatjana
AU  - Pljevljakušić, Dejan
AU  - Nikodinović-Runić, Jasmina
AU  - Bigović, Dubravka
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5642
AB  - Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii
VL  - 216
DO  - 10.1016/j.indcrop.2024.118762
ER  - 

@article{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Radan, Milica and Milivojević, Dušan and Stević, Tatjana and Pljevljakušić, Dejan and Nikodinović-Runić, Jasmina and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Within the global rise of antimicrobial resistance enhanced by the COVID-19 pandemic, where Acinetobacter baumannii has been distinguished as an emerging multi-resistant pathogen, essential oils, become the focus of novel therapeutic approaches. Hypothesizing that encapsulated Origanum heracleoticum L. and Thymus vulgaris L. essential oils could express multi-target approach against A. baumannii, this study aimed to develop microencapsulated systems with optimal technological qualities using 2-hydroxypropyl-β-cyclodextrin as a carrier, and to evaluate their pharmacological potential against A. baumannii, and their morphological and physicochemical characteristics, safety and stability profiles. The highest yield and encapsulation efficiency were obtained with 1:10 essential oil to carrier, and 1.5:10 carrier to water w/w ratios. The formation of inclusion complexes was confirmed by Fourier Transform Infrared Spectroscopy. Both microencapsulates achieved improved homogeneity, particle surface, and thermal stability compared with the pure carrier. Dominant bioactive compounds (carvacrol and p-cymene in O. heracleoticum essential oil, and thymol and p-cymene in T. vulgaris essential oil) remained the most abundant after encapsulation. While non-encapsulated essential oils revealed similar antimicrobial activity towards clinical A. baumannii isolates obtained from COVID-19 patients, encapsulated O. heracleoticum essential oil inhibited the bacterial growth at lower concentrations than T. vulgaris essential oil microencapsulate. All samples significantly reduced the formation of A. baumannii biofilm, for at least 53.90% towards the most infective isolate according to the Caenorhabditis elegans assay. Further, in silico molecular docking study revealed strong interaction pattern of carvacrol and thymol with the outer membrane protein A, which is the main factor for the A. baumannii biofilm formation. Cytotoxicity investigation on human lung A549 cells showed high survival rate in the presence of all tested concentrations, and the stability study revealed notable preservation of the bioactives’ content and pharmacological potential. Altogether, microencapsulated essential oils exhibited a multi-target approach towards A. baumannii, with satisfactory preserving capability during storage.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii",
volume = "216",
doi = "10.1016/j.indcrop.2024.118762"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Radan, M., Milivojević, D., Stević, T., Pljevljakušić, D., Nikodinović-Runić, J., Bigović, D., Šavikin, K.,& Filipić, B.. (2024). Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products
Elsevier B.V.., 216.
https://doi.org/10.1016/j.indcrop.2024.118762

Kuzmanović Nedeljković S, Ćujić Nikolić N, Radan M, Milivojević D, Stević T, Pljevljakušić D, Nikodinović-Runić J, Bigović D, Šavikin K, Filipić B. Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii. in Industrial Crops and Products. 2024;216.
doi:10.1016/j.indcrop.2024.118762 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Radan, Milica, Milivojević, Dušan, Stević, Tatjana, Pljevljakušić, Dejan, Nikodinović-Runić, Jasmina, Bigović, Dubravka, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Origanum heracleoticum L. and Thymus vulgaris L. essential oils – Novel strategy to combat multi-resistant Acinetobacter baumannii" in Industrial Crops and Products, 216 (2024),
https://doi.org/10.1016/j.indcrop.2024.118762 . .

TY  - JOUR
AU  - Krmar, Jovana
AU  - Svrkota, Bojana
AU  - Obradović, Darija
AU  - Vlatković, Vladimir
AU  - Lazović, Saša
AU  - Otašević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5634
AB  - Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice.
AB  - Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis
T1  - Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi
VL  - 74
IS  - 2
SP  - 133
EP  - 159
DO  - 10.5937/arhfarm74-49565
ER  - 

@article{
author = "Krmar, Jovana and Svrkota, Bojana and Obradović, Darija and Vlatković, Vladimir and Lazović, Saša and Otašević, Biljana",
year = "2024",
abstract = "Initially employed primarily at a preparative scale for enantiomer separation of chiral drug
candidates, Supercritical Fluid Chromatography (SFC) is nowadays extensively used in the
analytical mode. Recent advances in SFC separation science have emphasized its potential for
modern and environmentally friendly pharmaceutical analysis.
The aim of this review is to provide a deeper insight into the main fundamental and practical
aspects of the SFC technique in order to familiarize readers with its versatile nature and efficiency
in creating sustainable chromatographic solutions. All considerations are made primarily in the
context of the most widely used mode of operation - achiral SFC. In addition, recent applications
of this promising technique are presented at the end of the article to further promote its use in
pharmaceutical analytical practice., Na početku pretežno korišćena kao preparativna tehnika u enantioseparaciji hiralnih
molekula kandidatâ za lek, superkritična fluidna hromatografija (eng. supercritical fluid
chromatography, SFC) danas se široko koristi u analitičke svrhe. Noviji naučni napori ukazali su
na značaj SFC tehnike u modernoj i ekološki prihvatljivoj farmaceutskoj analizi.
Cilj ovog preglednog rada je pružanje dubljeg uvida u najvažnije fundamentalne i praktične
aspekte SFC tehnike, kako bi se čitaocima približio njen svestrani karakter, te efikasnost u
kreiranju održivih hromatografskih rešenja. Sva razmatranja prevashodno su data u kontekstu
najzastupljenijeg režima rada - ahiralne SFC. Takođe, na kraju rada predstavljene su savremene
primene ove obećavajuće tehnike kako bi se dodatno ohrabrilo njeno usvajanje u farmaceutsku
analitičku praksu.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis, Efikasnost superkritične fluidne hromatografije u kreiranju ekološki prihvatljivih rešenja u farmaceutskoj analizi",
volume = "74",
number = "2",
pages = "133-159",
doi = "10.5937/arhfarm74-49565"
}

Krmar, J., Svrkota, B., Obradović, D., Vlatković, V., Lazović, S.,& Otašević, B.. (2024). Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 133-159.
https://doi.org/10.5937/arhfarm74-49565

Krmar J, Svrkota B, Obradović D, Vlatković V, Lazović S, Otašević B. Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis. in Arhiv za farmaciju. 2024;74(2):133-159.
doi:10.5937/arhfarm74-49565 .

Krmar, Jovana, Svrkota, Bojana, Obradović, Darija, Vlatković, Vladimir, Lazović, Saša, Otašević, Biljana, "Leveraging the Power of Supercritical Fluid Chromatography for Eco-Conscious Solutions in Pharmaceutical Analysis" in Arhiv za farmaciju, 74, no. 2 (2024):133-159,
https://doi.org/10.5937/arhfarm74-49565 . .

TY  - JOUR
AU  - Đajić, Nevena
AU  - Krmar, Jovana
AU  - Stojanović, Jevrem
AU  - Svrkota, Bojana
AU  - Otašević, Biljana
AU  - Malenović, Anđelija
AU  - Protić, Ana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5640
AB  - Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion.
AB  - U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products
T1  - 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda
VL  - 74
IS  - 2
SP  - 235
EP  - 266
DO  - 10.5937/arhfarm74-49572
ER  - 

@article{
author = "Đajić, Nevena and Krmar, Jovana and Stojanović, Jevrem and Svrkota, Bojana and Otašević, Biljana and Malenović, Anđelija and Protić, Ana",
year = "2024",
abstract = "Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), β-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and β-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion., U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u značajnom porastu. Reverzno-fazna tečna hromatografija visokih
performansi (RP-HPLC) kao dominantno korišćena tehnika u velikoj meri se oslanja na primenu
organskih rastvarača, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksičnosti i zapaljivosti. Kako bi se ovi problemi rešili i zaštitilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksičnih organskih rastvarača svesti na minimum. Cilj ovog istraživanja
bio je da preporuči „zelenije“ modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, uključujući micelarnu tečnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
β-ciklodekstrinom (CD) i tečnu hromatografiju ultra visokih performansi (UHPLC). Ekološka
prihvatljivost ovih metoda je procenjena korišćenjem analitičke eko-skale, indeksa zelene
analitičke procedure (GAPI) i pristupa analitičke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveće razlike između navedenih metoda, kao i uvid u kritične
aspekte metoda. UHPLC i β-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda će biti metoda izbora zavisi od toga da li se lakoća implementacije
ili energetska efikasnost smatraju važnijim kriterijumom.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products, 266 Koncept ekološki prihvatljivih hromatografskih metoda: Studija slučaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda",
volume = "74",
number = "2",
pages = "235-266",
doi = "10.5937/arhfarm74-49572"
}

Đajić, N., Krmar, J., Stojanović, J., Svrkota, B., Otašević, B., Malenović, A.,& Protić, A.. (2024). Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 235-266.
https://doi.org/10.5937/arhfarm74-49572

Đajić N, Krmar J, Stojanović J, Svrkota B, Otašević B, Malenović A, Protić A. Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products. in Arhiv za farmaciju. 2024;74(2):235-266.
doi:10.5937/arhfarm74-49572 .

Đajić, Nevena, Krmar, Jovana, Stojanović, Jevrem, Svrkota, Bojana, Otašević, Biljana, Malenović, Anđelija, Protić, Ana, "Concept of ecologically acceptable chromatographic methods: Case study on the separation of dronedarone hydrochloride and its degradation products" in Arhiv za farmaciju, 74, no. 2 (2024):235-266,
https://doi.org/10.5937/arhfarm74-49572 . .

TY  - JOUR
AU  - Ravnikar, Matjaž
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
AU  - Sirše, Mateja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5641
AB  - The present study screened various fungal species for inhibitors of alpha-glucosidase, alpha-amylase, and DPP-4, enzymes that are crucial in carbohydrate metabolism. Ethanolic extracts exhibited superior inhibitory activity compared to water extracts, suggesting their potential as sources of anti-diabetic agents. Further fractionation revealed fomentariol from Fomes fomentarius as a potent inhibitor of alpha-glucosidase and DPP-4, with higher activity against alpha glucosidase than acarbose. Fomentariol presents a novel avenue for diabetes management, demonstrating the simultaneous inhibition of key enzymes in glucose metabolism. However, comprehensive clinical studies are needed to evaluate its safety and efficacy in humans.
PB  - MDPI
T2  - Nutraceuticals
T1  - Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis
VL  - 4
IS  - 2
SP  - 273
EP  - 282
DO  - 10.3390/nutraceuticals4020017
ER  - 

@article{
author = "Ravnikar, Matjaž and Štrukelj, Borut and Otašević, Biljana and Sirše, Mateja",
year = "2024",
abstract = "The present study screened various fungal species for inhibitors of alpha-glucosidase, alpha-amylase, and DPP-4, enzymes that are crucial in carbohydrate metabolism. Ethanolic extracts exhibited superior inhibitory activity compared to water extracts, suggesting their potential as sources of anti-diabetic agents. Further fractionation revealed fomentariol from Fomes fomentarius as a potent inhibitor of alpha-glucosidase and DPP-4, with higher activity against alpha glucosidase than acarbose. Fomentariol presents a novel avenue for diabetes management, demonstrating the simultaneous inhibition of key enzymes in glucose metabolism. However, comprehensive clinical studies are needed to evaluate its safety and efficacy in humans.",
publisher = "MDPI",
journal = "Nutraceuticals",
title = "Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis",
volume = "4",
number = "2",
pages = "273-282",
doi = "10.3390/nutraceuticals4020017"
}

Ravnikar, M., Štrukelj, B., Otašević, B.,& Sirše, M.. (2024). Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis. in Nutraceuticals
MDPI., 4(2), 273-282.
https://doi.org/10.3390/nutraceuticals4020017

Ravnikar M, Štrukelj B, Otašević B, Sirše M. Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis. in Nutraceuticals. 2024;4(2):273-282.
doi:10.3390/nutraceuticals4020017 .

Ravnikar, Matjaž, Štrukelj, Borut, Otašević, Biljana, Sirše, Mateja, "Fomentariol, a Fomes fomentarius Compound, Exhibits Anti-Diabetic Effects in Fungal Material: An In Vitro Analysis" in Nutraceuticals, 4, no. 2 (2024):273-282,
https://doi.org/10.3390/nutraceuticals4020017 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Vladimirov, Sandra
AU  - Munjas, Jelena
AU  - Mitić, Tijana
AU  - Hall, Ignacio Fernando
AU  - Jušić, Amela
AU  - Ružić, Dušan
AU  - Devaux, Yvan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5637
AB  - Noncoding RNAs (ncRNAs) are pivotal for various pathological processes, impacting disease progression. The potential for leveraging ncRNAs to prevent or treat atherosclerosis and associated cardiovascular diseases is of great significance, especially given the increasing prevalence of atherosclerosis in an ageing and sedentary population. Together, these diseases impose a substantial socio-economic burden, demanding innovative therapeutic solutions. This review explores the potential of ncRNAs in atherosclerosis treatment. We commence by examining approaches for identifying and characterizing atherosclerosis-associated ncRNAs. We then delve into the functional aspects of ncRNAs in atherosclerosis development and progression. Additionally, we review current RNA and RNA-targeting molecules in development or under approval for clinical use, offering insights into their pharmacological potential. The importance of improved ncRNA delivery strategies is highlighted. Finally, we suggest avenues for advanced research to accelerate the use of ncRNAs in treating atherosclerosis and mitigating its societal impact.
PB  - John Wiley and Sons Inc
T2  - British Journal of Pharmacology
T1  - Targeting noncoding RNAs to treat atherosclerosis
DO  - 10.1111/bph.16412
ER  - 

@article{
author = "Sopić, Miron and Vladimirov, Sandra and Munjas, Jelena and Mitić, Tijana and Hall, Ignacio Fernando and Jušić, Amela and Ružić, Dušan and Devaux, Yvan",
year = "2024",
abstract = "Noncoding RNAs (ncRNAs) are pivotal for various pathological processes, impacting disease progression. The potential for leveraging ncRNAs to prevent or treat atherosclerosis and associated cardiovascular diseases is of great significance, especially given the increasing prevalence of atherosclerosis in an ageing and sedentary population. Together, these diseases impose a substantial socio-economic burden, demanding innovative therapeutic solutions. This review explores the potential of ncRNAs in atherosclerosis treatment. We commence by examining approaches for identifying and characterizing atherosclerosis-associated ncRNAs. We then delve into the functional aspects of ncRNAs in atherosclerosis development and progression. Additionally, we review current RNA and RNA-targeting molecules in development or under approval for clinical use, offering insights into their pharmacological potential. The importance of improved ncRNA delivery strategies is highlighted. Finally, we suggest avenues for advanced research to accelerate the use of ncRNAs in treating atherosclerosis and mitigating its societal impact.",
publisher = "John Wiley and Sons Inc",
journal = "British Journal of Pharmacology",
title = "Targeting noncoding RNAs to treat atherosclerosis",
doi = "10.1111/bph.16412"
}

Sopić, M., Vladimirov, S., Munjas, J., Mitić, T., Hall, I. F., Jušić, A., Ružić, D.,& Devaux, Y.. (2024). Targeting noncoding RNAs to treat atherosclerosis. in British Journal of Pharmacology
John Wiley and Sons Inc..
https://doi.org/10.1111/bph.16412

Sopić M, Vladimirov S, Munjas J, Mitić T, Hall IF, Jušić A, Ružić D, Devaux Y. Targeting noncoding RNAs to treat atherosclerosis. in British Journal of Pharmacology. 2024;.
doi:10.1111/bph.16412 .

Sopić, Miron, Vladimirov, Sandra, Munjas, Jelena, Mitić, Tijana, Hall, Ignacio Fernando, Jušić, Amela, Ružić, Dušan, Devaux, Yvan, "Targeting noncoding RNAs to treat atherosclerosis" in British Journal of Pharmacology (2024),
https://doi.org/10.1111/bph.16412 . .

TY  - JOUR
AU  - Samardžić, Stevan
AU  - Đorđić Crnogorac, Marija
AU  - Petković, Miloš
AU  - Arsenijević, Jelena
AU  - Stanojković, Tatjana
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5636
AB  - This study employed the MTT assay to assess the cytotoxicity of one flavan and two stilbene derivatives isolated from the false indigo-bush (Amorpha fruticosa L.) fruits: 5,7-dihydroxy-8-geranylflavanone (1), 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (2), and 2-carboxy-3-hydroxy-
4-prenyl-5-methoxybibenzyl (3). The examined compounds reduced the survival of human cervical and colon tumour cells (HeLa, HT-29, HCT-116, and LS174) with IC50 values ranging from 10.55 to 147.09 μg/mL, except for 1, which did not affect LS174 cells within the tested concentrations. The highest activity was observed for 1 against HeLa cells, and 1 also exhibited the weakest effect against normal foetal lung fibroblasts (IC50 = 166.11 μg/mL), demonstrating good potency and selectivity. Stilbenes 2 and 3 proved efficacious, but lacked selectivity compared to 1. Our findings revealed the cytotoxicity of false indigo-bush constituents, justifying further mechanistic and in vivo investigations, particularly on 5,7-dihydroxy-8-geranylflavanone, which displayed considerable in vitro anticancer capacity and a potentially favourable safety profile.
PB  - Taylor & Francis Ltd.
T2  - Natural Product Research
T1  - Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)
DO  - 10.1080/14786419.2024.2353912
ER  - 

@article{
author = "Samardžić, Stevan and Đorđić Crnogorac, Marija and Petković, Miloš and Arsenijević, Jelena and Stanojković, Tatjana and Maksimović, Zoran",
year = "2024",
abstract = "This study employed the MTT assay to assess the cytotoxicity of one flavan and two stilbene derivatives isolated from the false indigo-bush (Amorpha fruticosa L.) fruits: 5,7-dihydroxy-8-geranylflavanone (1), 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (2), and 2-carboxy-3-hydroxy-
4-prenyl-5-methoxybibenzyl (3). The examined compounds reduced the survival of human cervical and colon tumour cells (HeLa, HT-29, HCT-116, and LS174) with IC50 values ranging from 10.55 to 147.09 μg/mL, except for 1, which did not affect LS174 cells within the tested concentrations. The highest activity was observed for 1 against HeLa cells, and 1 also exhibited the weakest effect against normal foetal lung fibroblasts (IC50 = 166.11 μg/mL), demonstrating good potency and selectivity. Stilbenes 2 and 3 proved efficacious, but lacked selectivity compared to 1. Our findings revealed the cytotoxicity of false indigo-bush constituents, justifying further mechanistic and in vivo investigations, particularly on 5,7-dihydroxy-8-geranylflavanone, which displayed considerable in vitro anticancer capacity and a potentially favourable safety profile.",
publisher = "Taylor & Francis Ltd.",
journal = "Natural Product Research",
title = "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)",
doi = "10.1080/14786419.2024.2353912"
}

Samardžić, S., Đorđić Crnogorac, M., Petković, M., Arsenijević, J., Stanojković, T.,& Maksimović, Z.. (2024). Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in Natural Product Research
Taylor & Francis Ltd...
https://doi.org/10.1080/14786419.2024.2353912

Samardžić S, Đorđić Crnogorac M, Petković M, Arsenijević J, Stanojković T, Maksimović Z. Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.). in Natural Product Research. 2024;.
doi:10.1080/14786419.2024.2353912 .

Samardžić, Stevan, Đorđić Crnogorac, Marija, Petković, Miloš, Arsenijević, Jelena, Stanojković, Tatjana, Maksimović, Zoran, "Cytotoxic prenylated phenols of false indigo-bush (Amorpha fruticosa L.)" in Natural Product Research (2024),
https://doi.org/10.1080/14786419.2024.2353912 . .

TY  - JOUR
AU  - Milaković, Dragana
AU  - Kovačević, Tijana
AU  - Kovačević, Peđa
AU  - Barišić, Vedrana
AU  - Avram, Sanja
AU  - Dragić, Saša
AU  - Zlojutro, Biljana
AU  - Momčičević, Danica
AU  - Miljković, Branislava
AU  - Vučićević, Katarina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5557
AB  - During veno-venous extracorporeal membrane oxygenation (vv ECMO) therapy, antimicrobial drugs are frequently used, and appropriate dosing is challenging due to there being limited data to support the dosage. Linezolid is effective against multidrug-resistant Gram-positive pathogens frequently isolated in ECMO patients. In total, 53 steady-state linezolid levels were obtained following 600 mg intravenous (IV) injections every 8 h, and these were used to develop a population pharmacokinetic (PopPK) model in patients with COVID-19-associated acute respiratory distress syndrome (CARDS) on vv ECMO. The data were analyzed using a nonlinear mixed-effects modelling approach. Monte Carlo simulation generated 5000 patients’ individual PK parameters and corresponding concentration–time profiles using the PopPK model, following the administration of 600 mg/8 h (a higher-than-standard dosing) and 600 mg/12 h (standard). The probabilities of pharmacokinetic/pharmacodynamic (PK/PD) target attainment (PTA) and the cumulative fraction of responses (CFR) for three pathogens were calculated and compared between the two dosing scenarios. Linezolid 600 mg/8 h was predicted to achieve greater than or equal to 85%Tf>MIC in at least 90% of the patients with CARDS on vv ECMO compared to only approximately two thirds of the patients after dosing every 12 h at a minimal inhibitory concentration (MIC) of 2 mg/L. In addition, for the same MIC, fAUC24/MIC ≥ 80 was achieved in almost three times the number of patients following an 8-h versus a 12-h interval. PopPK simulation predicted that a significantly higher proportion of the patients with CARDS on vv ECMO would achieve the PK/PD targets following the 8-h dosing interval compared to standard linezolid dosing. Nevertheless, the safety concern, in particular, for thrombocytopenia, with higher-than-standard linezolid dosage is reasonable, and consequently, monitoring is essential.
PB  - MDPI
T2  - Pharmaceutics
T1  - Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing
VL  - 16
IS  - 2
DO  - 10.3390/pharmaceutics16020253
ER  - 

@article{
author = "Milaković, Dragana and Kovačević, Tijana and Kovačević, Peđa and Barišić, Vedrana and Avram, Sanja and Dragić, Saša and Zlojutro, Biljana and Momčičević, Danica and Miljković, Branislava and Vučićević, Katarina",
year = "2024",
abstract = "During veno-venous extracorporeal membrane oxygenation (vv ECMO) therapy, antimicrobial drugs are frequently used, and appropriate dosing is challenging due to there being limited data to support the dosage. Linezolid is effective against multidrug-resistant Gram-positive pathogens frequently isolated in ECMO patients. In total, 53 steady-state linezolid levels were obtained following 600 mg intravenous (IV) injections every 8 h, and these were used to develop a population pharmacokinetic (PopPK) model in patients with COVID-19-associated acute respiratory distress syndrome (CARDS) on vv ECMO. The data were analyzed using a nonlinear mixed-effects modelling approach. Monte Carlo simulation generated 5000 patients’ individual PK parameters and corresponding concentration–time profiles using the PopPK model, following the administration of 600 mg/8 h (a higher-than-standard dosing) and 600 mg/12 h (standard). The probabilities of pharmacokinetic/pharmacodynamic (PK/PD) target attainment (PTA) and the cumulative fraction of responses (CFR) for three pathogens were calculated and compared between the two dosing scenarios. Linezolid 600 mg/8 h was predicted to achieve greater than or equal to 85%Tf>MIC in at least 90% of the patients with CARDS on vv ECMO compared to only approximately two thirds of the patients after dosing every 12 h at a minimal inhibitory concentration (MIC) of 2 mg/L. In addition, for the same MIC, fAUC24/MIC ≥ 80 was achieved in almost three times the number of patients following an 8-h versus a 12-h interval. PopPK simulation predicted that a significantly higher proportion of the patients with CARDS on vv ECMO would achieve the PK/PD targets following the 8-h dosing interval compared to standard linezolid dosing. Nevertheless, the safety concern, in particular, for thrombocytopenia, with higher-than-standard linezolid dosage is reasonable, and consequently, monitoring is essential.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing",
volume = "16",
number = "2",
doi = "10.3390/pharmaceutics16020253"
}

Milaković, D., Kovačević, T., Kovačević, P., Barišić, V., Avram, S., Dragić, S., Zlojutro, B., Momčičević, D., Miljković, B.,& Vučićević, K.. (2024). Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing. in Pharmaceutics
MDPI., 16(2).
https://doi.org/10.3390/pharmaceutics16020253

Milaković D, Kovačević T, Kovačević P, Barišić V, Avram S, Dragić S, Zlojutro B, Momčičević D, Miljković B, Vučićević K. Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing. in Pharmaceutics. 2024;16(2).
doi:10.3390/pharmaceutics16020253 .

Milaković, Dragana, Kovačević, Tijana, Kovačević, Peđa, Barišić, Vedrana, Avram, Sanja, Dragić, Saša, Zlojutro, Biljana, Momčičević, Danica, Miljković, Branislava, Vučićević, Katarina, "Population Pharmacokinetic Model of Linezolid and Probability of Target Attainment in Patients with COVID-19-Associated Acute Respiratory Distress Syndrome on Veno-Venous Extracorporeal Membrane Oxygenation—A Step toward Correct Dosing" in Pharmaceutics, 16, no. 2 (2024),
https://doi.org/10.3390/pharmaceutics16020253 . .

TY  - JOUR
AU  - Vasilić, Đorđe
AU  - Rašević, Marija
AU  - Zečević, Mira
AU  - Čarapić, Marija
AU  - Malenović, Anđelija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5633
AB  - In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis.
AB  - Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
PB  - Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Arhiv za farmaciju
T1  - Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis
T1  - Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova
VL  - 74
IS  - 2
SP  - 160
EP  - 177
DO  - 10.5937/arhfarm74-49545
ER  - 

@article{
author = "Vasilić, Đorđe and Rašević, Marija and Zečević, Mira and Čarapić, Marija and Malenović, Anđelija",
year = "2024",
abstract = "In recent years, Deep Eutectic Solvents (DESs) have attracted increasing attention in
various scientific disciplines. Their unique properties, such as low toxicity, biodegradability, low
cost and versatility, have made them an attractive alternative to conventional organic solvents. In
pharmaceutical analysis, where the demand for environmentally friendly methods is growing,
questions regarding whether DESs can replace organic solvents as "green solvents" have arisen.
This paper explores the potential applications of DESs in drug analysis, and highlights the benefits
and challenges they bring. Examples of the use of DESs in chromatographic techniques and in
the extraction of drugs from various samples are presented. Additionally, research on the stability
and toxicity of DESs in analytical applications is discussed. Based on the available data, it could
be concluded that DESs have the potential to become an important component of analytical
methods in pharmaceutical analysis and provide environmentally sustainable and efficient
alternatives to conventional organic solvents. However, further research is needed to better
understand their properties and optimize their application in drug analysis., Poslednjih godina, eutektički rastvarači privlače sve veću pažnju u različitim naučnim
disciplinama. Njihova jedinstvena svojstva, kao što su niska toksičnost, biorazgradivost, niska
cena i mogućnost primene u različitim oblastima, učinila su ih atraktivnom alternativom
konvencionalnim organskim rastvaračima. U farmaceutskoj analizi, gde su sve veći zahtevi za
ekološki prihvatljivim metodama, postavlja se pitanje da li eutektički rastvarači, kao „zeleni
rastvarači“, mogu da zamene organske rastvarače. Ovaj rad istražuje potencijalnu primenu
eutektičkih rastvarača u analizi lekova i ističe prednosti i izazove koje oni donose. Prikazani su
primeri upotrebe eutektičkih rastvarača u hromatografiji i u ekstrakciji lekova iz različitih
uzoraka. Pored toga, razmatrana je stabilnost i toksičnost eutektičkih rastvarača koji se koriste u
analitičkim metodama. Na osnovu dostupnih podataka, može se zaključiti da eutektički rastvarači
imaju značajan potencijal u pogledu upotrebe u analitičkim metodama u farmaceutskoj analizi i
pružanja ekološki održive i efikasne alternative konvencionalnim organskim rastvaračima.
Međutim, potrebna su dalja istraživanja kako bi se bolje razumela njihova svojstva i optimizovala
njihova primena u ispitivanju lekova.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd, Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Arhiv za farmaciju",
title = "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis, Mogućnost zamene konvencionalnih organskih rastvarača zelenim dubokim eutektičkim rastvaračima u analitici lekova",
volume = "74",
number = "2",
pages = "160-177",
doi = "10.5937/arhfarm74-49545"
}

Vasilić, Đ., Rašević, M., Zečević, M., Čarapić, M.,& Malenović, A.. (2024). Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 74(2), 160-177.
https://doi.org/10.5937/arhfarm74-49545

Vasilić Đ, Rašević M, Zečević M, Čarapić M, Malenović A. Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis. in Arhiv za farmaciju. 2024;74(2):160-177.
doi:10.5937/arhfarm74-49545 .

Vasilić, Đorđe, Rašević, Marija, Zečević, Mira, Čarapić, Marija, Malenović, Anđelija, "Possibility of replacing conventional organic solvents with green Deep Eutectic Solvents in drug analysis" in Arhiv za farmaciju, 74, no. 2 (2024):160-177,
https://doi.org/10.5937/arhfarm74-49545 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Stopa, Victoria
AU  - Devaux, Yvan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5432
PB  - Oxford University Press on behalf of the European Society of Cardiology
T2  - European Heart Journal
T1  - Leveraging epitranscriptomics for cardiovascular disease theranostics
VL  - 45
IS  - 13
SP  - 1098
EP  - 1100
DO  - 10.1093/eurheartj/ehad852
ER  - 

@article{
author = "Sopić, Miron and Stopa, Victoria and Devaux, Yvan",
year = "2024",
publisher = "Oxford University Press on behalf of the European Society of Cardiology",
journal = "European Heart Journal",
title = "Leveraging epitranscriptomics for cardiovascular disease theranostics",
volume = "45",
number = "13",
pages = "1098-1100",
doi = "10.1093/eurheartj/ehad852"
}

Sopić, M., Stopa, V.,& Devaux, Y.. (2024). Leveraging epitranscriptomics for cardiovascular disease theranostics. in European Heart Journal
Oxford University Press on behalf of the European Society of Cardiology., 45(13), 1098-1100.
https://doi.org/10.1093/eurheartj/ehad852

Sopić M, Stopa V, Devaux Y. Leveraging epitranscriptomics for cardiovascular disease theranostics. in European Heart Journal. 2024;45(13):1098-1100.
doi:10.1093/eurheartj/ehad852 .

Sopić, Miron, Stopa, Victoria, Devaux, Yvan, "Leveraging epitranscriptomics for cardiovascular disease theranostics" in European Heart Journal, 45, no. 13 (2024):1098-1100,
https://doi.org/10.1093/eurheartj/ehad852 . .

TY  - JOUR
AU  - Skrede, Silje
AU  - Bogavac-Stanojević, Nataša
AU  - Dreesen, Erwin
AU  - Nielsen, Elisabet
AU  - Zaninotto, Martina
AU  - Mulleman, Denis
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5532
AB  - The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.
PB  - John Wiley and Sons Inc
T2  - Basic and Clinical Pharmacology and Toxicology
T1  - Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe
DO  - 10.1111/bcpt.13983
ER  - 

@article{
author = "Skrede, Silje and Bogavac-Stanojević, Nataša and Dreesen, Erwin and Nielsen, Elisabet and Zaninotto, Martina and Mulleman, Denis",
year = "2024",
abstract = "The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.",
publisher = "John Wiley and Sons Inc",
journal = "Basic and Clinical Pharmacology and Toxicology",
title = "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe",
doi = "10.1111/bcpt.13983"
}

Skrede, S., Bogavac-Stanojević, N., Dreesen, E., Nielsen, E., Zaninotto, M.,& Mulleman, D.. (2024). Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology
John Wiley and Sons Inc..
https://doi.org/10.1111/bcpt.13983

Skrede S, Bogavac-Stanojević N, Dreesen E, Nielsen E, Zaninotto M, Mulleman D. Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology. 2024;.
doi:10.1111/bcpt.13983 .

Skrede, Silje, Bogavac-Stanojević, Nataša, Dreesen, Erwin, Nielsen, Elisabet, Zaninotto, Martina, Mulleman, Denis, "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe" in Basic and Clinical Pharmacology and Toxicology (2024),
https://doi.org/10.1111/bcpt.13983 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljević, Milica
AU  - Janković, Radmila
AU  - Đuretić, Jasmina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5626
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
PB  - Basel, Switzerland : MDPI AG
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
VL  - 12
IS  - 4
SP  - 717
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Biljana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljević, Milica and Janković, Radmila and Đuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.",
publisher = "Basel, Switzerland : MDPI AG",
journal = "Biomedicines",
title = "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats",
volume = "12",
number = "4",
pages = "717",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljević, M., Janković, R.,& Đuretić, J.. (2024). NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
Basel, Switzerland : MDPI AG., 12(4), 717.
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljević M, Janković R, Đuretić J. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4):717.
doi:10.3390/biomedicines12040717 .

Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024):717,
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Cilović Kozarević, Ermina
AU  - Dautović, Esmeralda
AU  - Halilčević, Dalila
AU  - Kolarević, Lamija
AU  - Šarić-Kundalić, Broza
AU  - Karić, Enida
AU  - Smajlagić, Aida
AU  - Husejnagić, Darja
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5635
AB  - The chemical composition of Pulicaria dysenterica (L.) Bernh. aerial parts essential oil (EO), growing wild in Bosnia and Herzegovina, was presented in the study. In addition to the EO composition, antimicrobial and cytotoxic activities were also tested. The aerial parts of P. dysenterica contained 0.3% of yellow, liquid, fragrant EO. The 51 components identified accounted for 81.09% of the oil. The EO was characterized by the presence of a high concentration of oxygenated sesquiterpenes 51.83% while oxygenated monoterpenes constituted 15.57%, sesquiterpene hydrocarbons 9.32% and non-terpene compounds presented 4.37% of the EO. The dominant compounds were the sesquiterpenes caryophyllene oxide, (E)-nerolidol, β-caryophyllene and monoterpene nerol. The antimicrobial activity of EO was tested against selected ATCC strains of microorganisms, the bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and the fungus Candida albicans. The results showed that the investigated EO inhibited the growth of all selected ATCC strains of microorganisms. The best result was obtained against Escherichia coli bacteria with MIC value of 1 mgmL-1. The cytotoxicity of EO was measured against the HeLa cell line using the MTT method with IC50 of 188.52 μgmL-1. This study has provided scientific baseline data on the therapeutic properties of P. dysenterica.
PB  - Faculty of Technology, University in Tuzla
T2  - Technologica Acta
T1  - Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)
VL  - 16
IS  - 2
SP  - 81
EP  - 86
DO  - 10.51558/2232-7568.2023.16.2.81
ER  - 

@article{
author = "Cilović Kozarević, Ermina and Dautović, Esmeralda and Halilčević, Dalila and Kolarević, Lamija and Šarić-Kundalić, Broza and Karić, Enida and Smajlagić, Aida and Husejnagić, Darja and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2024",
abstract = "The chemical composition of Pulicaria dysenterica (L.) Bernh. aerial parts essential oil (EO), growing wild in Bosnia and Herzegovina, was presented in the study. In addition to the EO composition, antimicrobial and cytotoxic activities were also tested. The aerial parts of P. dysenterica contained 0.3% of yellow, liquid, fragrant EO. The 51 components identified accounted for 81.09% of the oil. The EO was characterized by the presence of a high concentration of oxygenated sesquiterpenes 51.83% while oxygenated monoterpenes constituted 15.57%, sesquiterpene hydrocarbons 9.32% and non-terpene compounds presented 4.37% of the EO. The dominant compounds were the sesquiterpenes caryophyllene oxide, (E)-nerolidol, β-caryophyllene and monoterpene nerol. The antimicrobial activity of EO was tested against selected ATCC strains of microorganisms, the bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and the fungus Candida albicans. The results showed that the investigated EO inhibited the growth of all selected ATCC strains of microorganisms. The best result was obtained against Escherichia coli bacteria with MIC value of 1 mgmL-1. The cytotoxicity of EO was measured against the HeLa cell line using the MTT method with IC50 of 188.52 μgmL-1. This study has provided scientific baseline data on the therapeutic properties of P. dysenterica.",
publisher = "Faculty of Technology, University in Tuzla",
journal = "Technologica Acta",
title = "Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)",
volume = "16",
number = "2",
pages = "81-86",
doi = "10.51558/2232-7568.2023.16.2.81"
}

Cilović Kozarević, E., Dautović, E., Halilčević, D., Kolarević, L., Šarić-Kundalić, B., Karić, E., Smajlagić, A., Husejnagić, D., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2024). Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae). in Technologica Acta
Faculty of Technology, University in Tuzla., 16(2), 81-86.
https://doi.org/10.51558/2232-7568.2023.16.2.81

Cilović Kozarević E, Dautović E, Halilčević D, Kolarević L, Šarić-Kundalić B, Karić E, Smajlagić A, Husejnagić D, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae). in Technologica Acta. 2024;16(2):81-86.
doi:10.51558/2232-7568.2023.16.2.81 .

Cilović Kozarević, Ermina, Dautović, Esmeralda, Halilčević, Dalila, Kolarević, Lamija, Šarić-Kundalić, Broza, Karić, Enida, Smajlagić, Aida, Husejnagić, Darja, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)" in Technologica Acta, 16, no. 2 (2024):81-86,
https://doi.org/10.51558/2232-7568.2023.16.2.81 . .

TY  - JOUR
AU  - Bećirović, Sabina
AU  - Vuković, Bojana
AU  - Marinković, Valentina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5632
AB  - The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.
PB  - Taylor and Francis Ltd.
T2  - Biotechnology and Biotechnological Equipment
T1  - Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina
VL  - 38
IS  - 1
DO  - 10.1080/13102818.2024.2349593
ER  - 

@article{
author = "Bećirović, Sabina and Vuković, Bojana and Marinković, Valentina",
year = "2024",
abstract = "The healthcare structure in Bosnia and Herzegovina is decentralized, thus facing patients with unequal access to healthcare services especially for medicines. We attempted to develop a tool for assessment of the stakeholders’ opinion, and with the further research we will propose the model that can bring equality in Bosnia and Herzegovina. The developed tool will examine the attitudes and opinions for introducing new methods in the decision-making process during the listing of medicines for the reimbursement list in Bosnia and Herzegovina. An update of the country’s legislation can be presented based on the research results. The tool was developed using the Delphi method. The experts who were included in the Delphi panel are qualified for rating and discussing questions. The questionnaire was validated on a 5-point Likert scale, and additional comments or clarification are optional. Introductory interviews were held face to face with each expert individually; after that the panel was anonymous. After 3 rounds of Delphi, the created tool was checked through a pilot study. The developed tool was categorized into three groups based on the KAP survey (Knowledge, Attitudes, Perceptions). All questions were verified in pilot and with the results for Cronbach’s alpha 0.96. This shows sufficient reliability of the created questionnaire, and it can be administered to a larger group of respondents, which has been planned for further research. The findings provide reliable information useful for planning the country’s legislation updates and planning for the introduction of a new approach in the decision-making process.",
publisher = "Taylor and Francis Ltd.",
journal = "Biotechnology and Biotechnological Equipment",
title = "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina",
volume = "38",
number = "1",
doi = "10.1080/13102818.2024.2349593"
}

Bećirović, S., Vuković, B.,& Marinković, V.. (2024). Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment
Taylor and Francis Ltd.., 38(1).
https://doi.org/10.1080/13102818.2024.2349593

Bećirović S, Vuković B, Marinković V. Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina. in Biotechnology and Biotechnological Equipment. 2024;38(1).
doi:10.1080/13102818.2024.2349593 .

Bećirović, Sabina, Vuković, Bojana, Marinković, Valentina, "Tool for assessment of attitudes for applying a new approach in the decision-making process for reimbursement list in Bosnia and Herzegovina" in Biotechnology and Biotechnological Equipment, 38, no. 1 (2024),
https://doi.org/10.1080/13102818.2024.2349593 . .

TY  - JOUR
AU  - Adamov, Ivana
AU  - Stanojević, Gordana
AU  - Pavlović, Stefan
AU  - Medarević, Đorđe
AU  - Ivković, Branka
AU  - Kočović, David
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5630
AB  - Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets
VL  - 657
DO  - 10.1016/j.ijpharm.2024.124161
ER  - 

@article{
author = "Adamov, Ivana and Stanojević, Gordana and Pavlović, Stefan and Medarević, Đorđe and Ivković, Branka and Kočović, David and Ibrić, Svetlana",
year = "2024",
abstract = "Laser sintering, known as powder bed fusion–laser beam (PBF-LB), offers promising potential for the fabrication of patient-specific drugs. The aim of this study was to provide an insight into the PBF-LB process with regard to the process parameters, in particular the laser hatching distance, and its influence on the properties of zolpidem tartrate (ZT) tablets. PHARMACOAT® 603 was used as the polymer, while Candurin® Gold Sheen and AEROSIL® 200 were added to facilitate 3D printing. The particle size distribution of the powder blend showed that the layer height should be set to 100 µm, while the laser hatching distance was varied in five different steps (50, 100, 150, 200 and 250 µm), keeping the temperature and laser scanning speed constant. Increasing the laser hatching distance and decreasing the laser energy input led to a decrease in the colour intensity, mass, density and hardness of the ZT tablets, while the disintegration and dissolution rate were faster due to the more fragile bonds between the particles. The laser hatching distance also influenced the ZT dosage, indicating the importance of this process parameter in the production of presonalized drugs. The absence of drug-polymer interactions and the amorphization of the ZT were confirmed.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets",
volume = "657",
doi = "10.1016/j.ijpharm.2024.124161"
}

Adamov, I., Stanojević, G., Pavlović, S., Medarević, Đ., Ivković, B., Kočović, D.,& Ibrić, S.. (2024). Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics
Elsevier B.V.., 657.
https://doi.org/10.1016/j.ijpharm.2024.124161

Adamov I, Stanojević G, Pavlović S, Medarević Đ, Ivković B, Kočović D, Ibrić S. Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets. in International Journal of Pharmaceutics. 2024;657.
doi:10.1016/j.ijpharm.2024.124161 .

Adamov, Ivana, Stanojević, Gordana, Pavlović, Stefan, Medarević, Đorđe, Ivković, Branka, Kočović, David, Ibrić, Svetlana, "Powder bed fusion–laser beam (PBF-LB) three-dimensional (3D) printing: Influence of laser hatching distance on the properties of zolpidem tartrate tablets" in International Journal of Pharmaceutics, 657 (2024),
https://doi.org/10.1016/j.ijpharm.2024.124161 . .

TY  - JOUR
AU  - Obradović, Darija
AU  - Savić, Jelena
AU  - Joksimović, Jovana
AU  - Marković, Bojan
AU  - Vujić, Zorica
AU  - Lazović, Saša
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5549
AB  - Abstract: The retention profile of six serotonin receptor ligands such as arylpiperazines (aripiprazole, ziprasidone, risperidone), and benzothiazepine derivatives (olanzapine, mianserin, quetiapine), was investigated on C8 alkyl and pentafluorophenylpropyl stationary phases. The experimental design methodology was used to define their retention behavior and achieve acceptable separation results. Both phases showed satisfactory selectivity for all compounds. The optimal separation among the structurally related arylpiperazines and benzothiazepines was found with the C8 phase at 25°C, using acetonitrile (40%, v/v) and 20 mM ammonium acetate as a mobile phase modifier. The selectivity and sensitivity performances of the selected C8 system were discussed considering the separation of aripiprazole and its related compounds. In addition, the specified conditions were validated for determining aripiprazole in pharmaceutical dosage forms. The developed reversed-phase high-performance liquid chromatography method can be successfully used for the rapid chromatographic profiling of serotonin receptor ligands and related analogs, providing increased selectivity during pharmaceutical analysis.
PB  - Springer
T2  - Journal of Analytical Chemistry
T1  - Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds
VL  - 79
IS  - 1
SP  - 95
EP  - 104
DO  - 10.1134/S1061934824010076
ER  - 

@article{
author = "Obradović, Darija and Savić, Jelena and Joksimović, Jovana and Marković, Bojan and Vujić, Zorica and Lazović, Saša",
year = "2024",
abstract = "Abstract: The retention profile of six serotonin receptor ligands such as arylpiperazines (aripiprazole, ziprasidone, risperidone), and benzothiazepine derivatives (olanzapine, mianserin, quetiapine), was investigated on C8 alkyl and pentafluorophenylpropyl stationary phases. The experimental design methodology was used to define their retention behavior and achieve acceptable separation results. Both phases showed satisfactory selectivity for all compounds. The optimal separation among the structurally related arylpiperazines and benzothiazepines was found with the C8 phase at 25°C, using acetonitrile (40%, v/v) and 20 mM ammonium acetate as a mobile phase modifier. The selectivity and sensitivity performances of the selected C8 system were discussed considering the separation of aripiprazole and its related compounds. In addition, the specified conditions were validated for determining aripiprazole in pharmaceutical dosage forms. The developed reversed-phase high-performance liquid chromatography method can be successfully used for the rapid chromatographic profiling of serotonin receptor ligands and related analogs, providing increased selectivity during pharmaceutical analysis.",
publisher = "Springer",
journal = "Journal of Analytical Chemistry",
title = "Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds",
volume = "79",
number = "1",
pages = "95-104",
doi = "10.1134/S1061934824010076"
}

Obradović, D., Savić, J., Joksimović, J., Marković, B., Vujić, Z.,& Lazović, S.. (2024). Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds. in Journal of Analytical Chemistry
Springer., 79(1), 95-104.
https://doi.org/10.1134/S1061934824010076

Obradović D, Savić J, Joksimović J, Marković B, Vujić Z, Lazović S. Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds. in Journal of Analytical Chemistry. 2024;79(1):95-104.
doi:10.1134/S1061934824010076 .

Obradović, Darija, Savić, Jelena, Joksimović, Jovana, Marković, Bojan, Vujić, Zorica, Lazović, Saša, "Rapid Reversed-Phase High-Performance Liquid Chromatography Profiling of Serotonin Receptor Ligands and their Related Compounds" in Journal of Analytical Chemistry, 79, no. 1 (2024):95-104,
https://doi.org/10.1134/S1061934824010076 . .

TY  - CHAP
AU  - Medarević, Đorđe
AU  - Krstić, Mirjana
AU  - Ibrić, Svetlana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5607
AB  - With the progress in medicine during the last several decades, it became clear
that the course of the disease and response to applied therapy differ to a great
extent between patients due to differences in genetic, anatomical, and physiological
characteristics of the patients. This brought into question the current
concept of therapy, so-called one size fits all, where similar drug doses have
been prescribed to a huge number of patients, with neglect of interindividual
differences between patients.
PB  - Elsevier Inc.
T2  - From Current to Future Trends in Pharmaceutical Technology
T1  - Fundamentals of 3D printing of pharmaceuticals
SP  - 1
EP  - 65
DO  - 10.1016/B978-0-323-91111-5.00001-9
ER  - 

@inbook{
author = "Medarević, Đorđe and Krstić, Mirjana and Ibrić, Svetlana",
year = "2024",
abstract = "With the progress in medicine during the last several decades, it became clear
that the course of the disease and response to applied therapy differ to a great
extent between patients due to differences in genetic, anatomical, and physiological
characteristics of the patients. This brought into question the current
concept of therapy, so-called one size fits all, where similar drug doses have
been prescribed to a huge number of patients, with neglect of interindividual
differences between patients.",
publisher = "Elsevier Inc.",
journal = "From Current to Future Trends in Pharmaceutical Technology",
booktitle = "Fundamentals of 3D printing of pharmaceuticals",
pages = "1-65",
doi = "10.1016/B978-0-323-91111-5.00001-9"
}

Medarević, Đ., Krstić, M.,& Ibrić, S.. (2024). Fundamentals of 3D printing of pharmaceuticals. in From Current to Future Trends in Pharmaceutical Technology
Elsevier Inc.., 1-65.
https://doi.org/10.1016/B978-0-323-91111-5.00001-9

Medarević Đ, Krstić M, Ibrić S. Fundamentals of 3D printing of pharmaceuticals. in From Current to Future Trends in Pharmaceutical Technology. 2024;:1-65.
doi:10.1016/B978-0-323-91111-5.00001-9 .

Medarević, Đorđe, Krstić, Mirjana, Ibrić, Svetlana, "Fundamentals of 3D printing of pharmaceuticals" in From Current to Future Trends in Pharmaceutical Technology (2024):1-65,
https://doi.org/10.1016/B978-0-323-91111-5.00001-9 . .

TY  - CONF
AU  - Medarević, Đorđe
AU  - Turković, Erna
AU  - Čežek, Maša
AU  - Ibrić, Svetlana
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5606
AB  - INTRODUCTION
Crop residues (CRs) represent a resource that is available in
virtually unlimited quantities, but still the largest part
remains unused. Unfortunately, CRs are usually burned in
the field, which causes air pollution, contributes to global
warming by emitting greenhouse gasses, hinders nutrient
recycling, and negatively affects soil microbes through
overheating and carbon loss [1]. One of the most important
value-added components that can be isolated from CRs is
microcrystalline cellulose (MCC), a common excipient in
tablet formulation. In this study, a comparative evaluation
of tableting properties of MCC obtained from wheat and
corn CRs by different chemical treatment was performed. ...
PB  - International Association for Pharmaceutical Technology (APV), Mainz, Germany
PB  - International Society of Drug Delivery Sciences and Technology (APGI), Lille, France
PB  - Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy
C3  - 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
T1  - Comparative evaluation of tableting properties of microcrystalline cellulose obtained from wheat and corn crop residues
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5606
ER  - 

@conference{
author = "Medarević, Đorđe and Turković, Erna and Čežek, Maša and Ibrić, Svetlana and Maksimović, Zoran",
year = "2024",
abstract = "INTRODUCTION
Crop residues (CRs) represent a resource that is available in
virtually unlimited quantities, but still the largest part
remains unused. Unfortunately, CRs are usually burned in
the field, which causes air pollution, contributes to global
warming by emitting greenhouse gasses, hinders nutrient
recycling, and negatively affects soil microbes through
overheating and carbon loss [1]. One of the most important
value-added components that can be isolated from CRs is
microcrystalline cellulose (MCC), a common excipient in
tablet formulation. In this study, a comparative evaluation
of tableting properties of MCC obtained from wheat and
corn CRs by different chemical treatment was performed. ...",
publisher = "International Association for Pharmaceutical Technology (APV), Mainz, Germany, International Society of Drug Delivery Sciences and Technology (APGI), Lille, France, Italian Society of Technology and Legislation (S.I.T.E.L.F), Milan, Italy",
journal = "14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria",
title = "Comparative evaluation of tableting properties of microcrystalline cellulose obtained from wheat and corn crop residues",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5606"
}

Medarević, Đ., Turković, E., Čežek, M., Ibrić, S.,& Maksimović, Z.. (2024). Comparative evaluation of tableting properties of microcrystalline cellulose obtained from wheat and corn crop residues. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria
International Association for Pharmaceutical Technology (APV), Mainz, Germany..
https://hdl.handle.net/21.15107/rcub_farfar_5606

Medarević Đ, Turković E, Čežek M, Ibrić S, Maksimović Z. Comparative evaluation of tableting properties of microcrystalline cellulose obtained from wheat and corn crop residues. in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria. 2024;.
https://hdl.handle.net/21.15107/rcub_farfar_5606 .

Medarević, Đorđe, Turković, Erna, Čežek, Maša, Ibrić, Svetlana, Maksimović, Zoran, "Comparative evaluation of tableting properties of microcrystalline cellulose obtained from wheat and corn crop residues" in 14th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 18 - 21 March 2024, Vienna, Austria (2024),
https://hdl.handle.net/21.15107/rcub_farfar_5606 .

TY  - JOUR
AU  - Tasić, Gordana
AU  - Mitrović, Nikola
AU  - Simić, Milena
AU  - Koravović, Mladen
AU  - Jovanović, Predrag
AU  - Petković, Miloš
AU  - Jovanović, Miloš
AU  - Ivković, Branka
AU  - Savić, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5556
AB  - Hydantoin derivatives are versatile structural motifs found in natural products
and various compounds with different biological or other properties. Due to
their importance in both organic and medicinal chemistry, a number of synthetic procedures have been developed. In this article, a novel methodology
utilizing N-Boc protected amino acid amides for their preparation has been
described. The cyclisation process was accomplished using solid supported
PPh3 and CBr 4 as reagents affording substituted hydantoins in moderate to
good yields (40%–77%).
PB  - Wiley Periodicals LLC.
T2  - Journal Heterocyclic Chemistry
T1  - Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent
DO  - 10.1002/jhet.4802
ER  - 

@article{
author = "Tasić, Gordana and Mitrović, Nikola and Simić, Milena and Koravović, Mladen and Jovanović, Predrag and Petković, Miloš and Jovanović, Miloš and Ivković, Branka and Savić, Vladimir",
year = "2024",
abstract = "Hydantoin derivatives are versatile structural motifs found in natural products
and various compounds with different biological or other properties. Due to
their importance in both organic and medicinal chemistry, a number of synthetic procedures have been developed. In this article, a novel methodology
utilizing N-Boc protected amino acid amides for their preparation has been
described. The cyclisation process was accomplished using solid supported
PPh3 and CBr 4 as reagents affording substituted hydantoins in moderate to
good yields (40%–77%).",
publisher = "Wiley Periodicals LLC.",
journal = "Journal Heterocyclic Chemistry",
title = "Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent",
doi = "10.1002/jhet.4802"
}

Tasić, G., Mitrović, N., Simić, M., Koravović, M., Jovanović, P., Petković, M., Jovanović, M., Ivković, B.,& Savić, V.. (2024). Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent. in Journal Heterocyclic Chemistry
Wiley Periodicals LLC...
https://doi.org/10.1002/jhet.4802

Tasić G, Mitrović N, Simić M, Koravović M, Jovanović P, Petković M, Jovanović M, Ivković B, Savić V. Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent. in Journal Heterocyclic Chemistry. 2024;.
doi:10.1002/jhet.4802 .

Tasić, Gordana, Mitrović, Nikola, Simić, Milena, Koravović, Mladen, Jovanović, Predrag, Petković, Miloš, Jovanović, Miloš, Ivković, Branka, Savić, Vladimir, "Synthesis of hydantoins from N-Boc protected aminoacid derived amides using polymer-supported PPh3/CBr4as a reagent" in Journal Heterocyclic Chemistry (2024),
https://doi.org/10.1002/jhet.4802 . .

TY  - JOUR
AU  - Oljačić, Slavica
AU  - Križman, Mitja
AU  - Popović-Nikolić, Marija
AU  - Vovk, Irena
AU  - Nikolić, Katarina
AU  - Agbaba, Danica
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5629
AB  - Background and Purpose: The ligands of the imidazoline and α-adrenergic receptors are mainly imidazoline and guanidine derivatives, known as centrally-acting antihypertensives and compounds with potential use in various neurological disorders. The extent of their ionisation has a major influence on their behaviour in the different analytical systems. The main objective of this work was to compare the mechanism of chromatographic retention and electrophoretic mobility under acidic, neutral and basic conditions. Experimental Approach: Multiple Linear Regression and Partial Least Squares Regression were applied for the QSRR (quantitative structure-retention relationship) and QSMR (quantitative structure-mobility relationship) modelling and to select the most important molecular parameters describing the chromatographic and electrophoretic behaviour of the investigated compounds. Key Results: The most important molecular descriptors, such as the chemical composition of the compounds, lipophilicity, polarizability and molecular branching, in the selected QSRR models showed that an important insight into the retention behaviour can be derived from the 0D-, 1D- and 2D-descriptors. The electrophoretic mobility could be explained by 2D- and 3D-descriptors, which provide information on the molecular mass, size and complexity, as well as on the influence of charge transfer and electronic properties on the migration behaviour. Conclusion: All created QSRR/QSMR models met the stringent validation criteria and showed high potential in describing the chromatographic and electrophoretic behaviour of investigated compounds.
PB  - International Association of Physical Chemists
T2  - ADMET and DMPK
T1  - Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions
DO  - 10.5599/admet.2278
ER  - 

@article{
author = "Oljačić, Slavica and Križman, Mitja and Popović-Nikolić, Marija and Vovk, Irena and Nikolić, Katarina and Agbaba, Danica",
year = "2024",
abstract = "Background and Purpose: The ligands of the imidazoline and α-adrenergic receptors are mainly imidazoline and guanidine derivatives, known as centrally-acting antihypertensives and compounds with potential use in various neurological disorders. The extent of their ionisation has a major influence on their behaviour in the different analytical systems. The main objective of this work was to compare the mechanism of chromatographic retention and electrophoretic mobility under acidic, neutral and basic conditions. Experimental Approach: Multiple Linear Regression and Partial Least Squares Regression were applied for the QSRR (quantitative structure-retention relationship) and QSMR (quantitative structure-mobility relationship) modelling and to select the most important molecular parameters describing the chromatographic and electrophoretic behaviour of the investigated compounds. Key Results: The most important molecular descriptors, such as the chemical composition of the compounds, lipophilicity, polarizability and molecular branching, in the selected QSRR models showed that an important insight into the retention behaviour can be derived from the 0D-, 1D- and 2D-descriptors. The electrophoretic mobility could be explained by 2D- and 3D-descriptors, which provide information on the molecular mass, size and complexity, as well as on the influence of charge transfer and electronic properties on the migration behaviour. Conclusion: All created QSRR/QSMR models met the stringent validation criteria and showed high potential in describing the chromatographic and electrophoretic behaviour of investigated compounds.",
publisher = "International Association of Physical Chemists",
journal = "ADMET and DMPK",
title = "Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions",
doi = "10.5599/admet.2278"
}

Oljačić, S., Križman, M., Popović-Nikolić, M., Vovk, I., Nikolić, K.,& Agbaba, D.. (2024). Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions. in ADMET and DMPK
International Association of Physical Chemists..
https://doi.org/10.5599/admet.2278

Oljačić S, Križman M, Popović-Nikolić M, Vovk I, Nikolić K, Agbaba D. Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions. in ADMET and DMPK. 2024;.
doi:10.5599/admet.2278 .

Oljačić, Slavica, Križman, Mitja, Popović-Nikolić, Marija, Vovk, Irena, Nikolić, Katarina, Agbaba, Danica, "Modelling of chromatographic and electrophoretic behaviour of imidazoline and alpha adrenergic receptors ligands under different acid-base conditions" in ADMET and DMPK (2024),
https://doi.org/10.5599/admet.2278 . .

TY  - JOUR
AU  - Popović-Nikolić, Marija
AU  - Čakar, Mira
AU  - Todorović, Nina
AU  - Nikolić, Katarina
AU  - Popović, Gordana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5589
AB  - The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.
PB  - Springer Nature
T2  - Chemical Papers
T1  - Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride
DO  - 10.1007/s11696-024-03399-1
ER  - 

@article{
author = "Popović-Nikolić, Marija and Čakar, Mira and Todorović, Nina and Nikolić, Katarina and Popović, Gordana",
year = "2024",
abstract = "The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.",
publisher = "Springer Nature",
journal = "Chemical Papers",
title = "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride",
doi = "10.1007/s11696-024-03399-1"
}

Popović-Nikolić, M., Čakar, M., Todorović, N., Nikolić, K.,& Popović, G.. (2024). Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers
Springer Nature..
https://doi.org/10.1007/s11696-024-03399-1

Popović-Nikolić M, Čakar M, Todorović N, Nikolić K, Popović G. Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers. 2024;.
doi:10.1007/s11696-024-03399-1 .

Popović-Nikolić, Marija, Čakar, Mira, Todorović, Nina, Nikolić, Katarina, Popović, Gordana, "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride" in Chemical Papers (2024),
https://doi.org/10.1007/s11696-024-03399-1 . .